pycom01g14580

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003355
Physical Location & Seq
Forward (+)
14708427 .. 14709713
1287 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g14580.1

Sequence Viewer

Length: 1287 bp
ATGGCAACGTGTTTGCTGGCTCTTTTCTATGGTTTTTACCGCCTCTATAAGTTACTTATCGGATGGAGAGACCAGTCATGCTATCTGCTGGCCTACGAGTGCCACTTGCCTCCAGACGACATGAAGCTCAGCACTGACTCATGCGTGAAAATCGTCTTGCGGAATAGGAATCTGGGATTGGATGAGTTCAGGTTTCTCTTGAAAACTATTACCAATTCGGGCATAGGCGAGGAAACTTACTGCGCAAGAAACATCATCGAAGGCCGAGAAGAAAATGCAACCCTAGAAGATGAACACGCGGAGATGGATGGCATCATCTTCGACATGCTGGACAAGCTTTTCGCTAGGGCTACATCAATTTCTCCGTCACAAATTGACATTCTTGTCGTCAATGTGTCGATGTTCTCCCCTGCACCCTCCCTATCGTCGCGTATAGTGAATCGTTACAAGATGAGGGACAACATCAAGAGCTTCAACCTCTCGGGAATGGGCTGCAGTGCGACCCTGATTGCCGTTGACGTTGTACAAAACTTGTTCAAGTCTCACAAGAACGCGAATGCCATCGTTGTAAGCACGGAATCCTTAGCTCCTCATTGGTATTGCGGCATAGAAAAATCCATGATGCTCACAAACTGTCTCTTCCGCTCGGGAGGGTGCGCGATGCTGTTCACGAACAACCAAGACCTAAAGCACCAAGCCAAGCTGAAATTAAACCATTTGGTACGAATCCACACTGGCTCGAACGACGGCGCATATAACTGTTGCGTACAGGTAGACGATGAGAGTGGACATCGAGGTTTCCGCCTTACCAAATACCTATTAAAAGCAGCAACTCAGGGTTTTACAATAAACCTCCAAGCTCTAGCACCAAAAGTGCTTTCACTAAAAGAAATACTTAGGTACCTTGTAGCCTCTAGGTTTAAAAATATAATAACTAGTATTAAAAGCCAACAGCCAAAAGTAGCTGAGGGAGTAGGGTTGAATCTCAAGACAGGAATAGAGCACTTCTGTATTCACCCTGGTGGAAGAGCAATCATAGATGGGATTGGGAAGAGCTTGGAGCTAAGTGACTATGATGTCGAGCCGTCAAGAATGGCGCTTCACCGGTTCGGAAACACATCCGCGGCCGGATTTTGGTACGCTTTGGGATACATGGAGGCAAAAAAGAGGCTGAAGAAGGGGAATAAGATTCTGATGAGTGGATTTGGAGCTGGTTTCAAGTGCAACAATATTGTGTGGGAAGTGATAAGGACTTGGATGATGTCAATGTGTGGAAAGACTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

429

Amino Acids

47.85

Weight (kDa)

9.01

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 23 - 304 1.1e-112 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 332 - 414 2e-12 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 335 - 406 3.2e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 383, 1076
Acc16I TGCGCA 1 cut(s) 244
Acc65I GGTACC 1 cut(s) 900
AccB1I GGYRCC 1 cut(s) 900
AccBSI CCGCTC 1 cut(s) 645
AccI GTMKAC 1 cut(s) 774
AccII CGCG 5 cut(s) 299, 430, 554, 659, 1124
AciI CCGC 8 cut(s) 40, 160, 299, 603, 643, 802, 1122, 1124
AcoI YGGCCR 1 cut(s) 1125
AcuI CTGAAG 1 cut(s) 1193
AfaI GTAC 5 cut(s) 525, 723, 768, 902, 1139
AfiI CCNNNNNNNGG 1 cut(s) 1134
AflIII ACRYGT 1 cut(s) 8
AgeI ACCGGT 1 cut(s) 1104
AgsI TTSAA 5 cut(s) 202, 475, 538, 982, 1219
AhlI ACTAGT 1 cut(s) 935
AjnI CCWGG 1 cut(s) 1018
AjuI GAANNNNNNNTTGG 4 cut(s) 161, 193, 862, 894
AleI CACNNNNGTG 1 cut(s) 1020
Alw21I GWGCWC 1 cut(s) 1005
Alw26I GTCTC 3 cut(s) 63, 546, 641
Ama87I CYCGRG 2 cut(s) 481, 646
AoxI GGCC 3 cut(s) 90, 262, 1125
ApeKI GCWGC 2 cut(s) 492, 827
ArsI GACNNNNNNTTYG 2 cut(s) 323, 355
AsiGI ACCGGT 1 cut(s) 1104
Asp718I GGTACC 1 cut(s) 900
AspLEI GCGC 4 cut(s) 245, 659, 752, 1099
AsuHPI GGTGA 2 cut(s) 1007, 1094
AvaI CYCGRG 2 cut(s) 481, 646
BaeI ACNNNNGTAYC 2 cut(s) 884, 917
BanI GGYRCC 1 cut(s) 900
Bbv12I GWGCWC 1 cut(s) 1005
BbvCI CCTCAGC 1 cut(s) 966
BbvI GCAGC 2 cut(s) 479, 839
BccI CCATC 5 cut(s) 57, 298, 302, 569, 1034
BceAI ACGGC 3 cut(s) 497, 763, 1069
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciT130I CCWGG 1 cut(s) 1020
BciVI GTATCC 1 cut(s) 1142
BcoDI GTCTC 3 cut(s) 63, 546, 641
BcuI ACTAGT 1 cut(s) 935
BfaI CTAG 5 cut(s) 284, 345, 863, 915, 936
BfmI CTRYAG 1 cut(s) 493
BfoI RGCGCY 1 cut(s) 1100
BfuI GTATCC 1 cut(s) 1142
BisI GCNGC 4 cut(s) 493, 604, 828, 1125
BlpI GCTNAGC 1 cut(s) 128
BlsI GCNGC 4 cut(s) 494, 605, 829, 1126
Bme1390I CCNGG 1 cut(s) 1020
BmeT110I CYCGRG 2 cut(s) 481, 646
BmiI GGNNCC 1 cut(s) 902
BmrFI CCNGG 1 cut(s) 1020
BmsI GCATC 3 cut(s) 321, 612, 651
BpmI CTGGAG 1 cut(s) 96
Bpu10I CCTNAGC 2 cut(s) 583, 966
Bpu1102I GCTNAGC 1 cut(s) 128
BpuEI CTTGAG 1 cut(s) 971
BsaI GGTCTC 1 cut(s) 63
BsaJI CCNNGG 2 cut(s) 1018, 1122
BsaWI WCCGGW 1 cut(s) 1104
Bsc4I CCNNNNNNNGG 1 cut(s) 1134
Bse118I RCCGGY 1 cut(s) 1104
Bse1I ACTGG 2 cut(s) 73, 739
BseBI CCWGG 1 cut(s) 1020
BseDI CCNNGG 2 cut(s) 1018, 1122
BseGI GGATG 5 cut(s) 68, 187, 313, 1118, 1263
BseLI CCNNNNNNNGG 1 cut(s) 1134
BseMII CTCAG 3 cut(s) 142, 848, 957
BseNI ACTGG 2 cut(s) 73, 739
BseRI GAGGAG 1 cut(s) 579
BseX3I CGGCCG 1 cut(s) 1125
BseXI GCAGC 2 cut(s) 479, 839
BsgI GTGCAG 1 cut(s) 396
Bsh1236I CGCG 5 cut(s) 299, 430, 554, 659, 1124
Bsh1285I CGRYCG 1 cut(s) 1128
BshFI GGCC 3 cut(s) 92, 264, 1127
BshNI GGYRCC 1 cut(s) 900
BshTI ACCGGT 1 cut(s) 1104
BsiEI CGRYCG 1 cut(s) 1128
BsiHKAI GWGCWC 1 cut(s) 1005
BsiHKCI CYCGRG 2 cut(s) 481, 646
BsiSI CCGG 2 cut(s) 1105, 1128
BslFI GGGAC 1 cut(s) 470
BslI CCNNNNNNNGG 1 cut(s) 1134
BsmAI GTCTC 3 cut(s) 63, 546, 641
BsmFI GGGAC 1 cut(s) 470
BsmI GAATGC 1 cut(s) 562
BsnI GGCC 3 cut(s) 92, 264, 1127
Bso31I GGTCTC 1 cut(s) 63
BsoBI CYCGRG 2 cut(s) 481, 646
Bsp1286I GDGCHC 1 cut(s) 1005
Bsp1407I TGTACA 1 cut(s) 523
Bsp1720I GCTNAGC 1 cut(s) 128
BspACI CCGC 8 cut(s) 40, 160, 299, 603, 643, 802, 1122, 1124
BspANI GGCC 3 cut(s) 92, 264, 1127
BspCNI CTCAG 3 cut(s) 141, 847, 958
BspFNI CGCG 5 cut(s) 299, 430, 554, 659, 1124
BspLI GGNNCC 1 cut(s) 902
BspMAI CTGCAG 1 cut(s) 497
BspQI GCTCTTC 2 cut(s) 1021, 1046
BspT107I GGYRCC 1 cut(s) 900
BspTNI GGTCTC 1 cut(s) 63
BsrBI CCGCTC 1 cut(s) 645
BsrFI RCCGGY 1 cut(s) 1104
BsrGI TGTACA 1 cut(s) 523
BsrI ACTGG 2 cut(s) 73, 739
BssAI RCCGGY 1 cut(s) 1104
BssECI CCNNGG 2 cut(s) 1018, 1122
Bst2UI CCWGG 1 cut(s) 1020
Bst4CI ACNGT 2 cut(s) 635, 761
Bst6I CTCTTC 3 cut(s) 644, 1021, 1046
BstAUI TGTACA 1 cut(s) 523
BstC8I GCNNGC 2 cut(s) 18, 90
BstDEI CTNAG 6 cut(s) 128, 583, 834, 896, 966, 1064
BstDSI CCRYGG 1 cut(s) 1122
BstF5I GGATG 5 cut(s) 68, 187, 313, 1118, 1263
BstFNI CGCG 5 cut(s) 299, 430, 554, 659, 1124
BstH2I RGCGCY 1 cut(s) 1100
BstHHI GCGC 4 cut(s) 245, 659, 752, 1099
BstMAI GTCTC 3 cut(s) 63, 546, 641
BstMCI CGRYCG 1 cut(s) 1128
BstMWI GCNNNNNNNGC 1 cut(s) 334
BstNI CCWGG 1 cut(s) 1020
BstNSI RCATGY 1 cut(s) 328
BstSCI CCNGG 1 cut(s) 1018
BstSFI CTRYAG 1 cut(s) 493
BstUI CGCG 5 cut(s) 299, 430, 554, 659, 1124
BstV1I GCAGC 2 cut(s) 479, 839
BstZI CGGCCG 1 cut(s) 1125
BsuI GTATCC 1 cut(s) 1142
BsuRI GGCC 3 cut(s) 92, 264, 1127
BtgI CCRYGG 1 cut(s) 1122
BtgZI GCGATG 1 cut(s) 674
BtsCI GGATG 5 cut(s) 68, 187, 313, 1118, 1263
BtsI GCAGTG 1 cut(s) 502
BtsIMutI CAGTG 3 cut(s) 132, 502, 732
Cac8I GCNNGC 2 cut(s) 18, 90
CfoI GCGC 4 cut(s) 245, 659, 752, 1099
Cfr10I RCCGGY 1 cut(s) 1104
Cfr42I CCGCGG 1 cut(s) 1125
Csp6I GTAC 5 cut(s) 524, 722, 767, 901, 1138
CspAI ACCGGT 1 cut(s) 1104
CviAII CATG 6 cut(s) 78, 121, 141, 325, 619, 1153
CviQI GTAC 5 cut(s) 524, 722, 767, 901, 1138
DdeI CTNAG 6 cut(s) 128, 583, 834, 896, 966, 1064
DraI TTTAAA 1 cut(s) 922
DrdI GACNNNNNNGTC 2 cut(s) 383, 1076
DseDI GACNNNNNNGTC 2 cut(s) 383, 1076
EaeI YGGCCR 1 cut(s) 1125
EagI CGGCCG 1 cut(s) 1125
Eam1104I CTCTTC 3 cut(s) 644, 1021, 1046
EarI CTCTTC 3 cut(s) 644, 1021, 1046
EciI GGCGGA 1 cut(s) 791
EclXI CGGCCG 1 cut(s) 1125
Eco31I GGTCTC 1 cut(s) 63
Eco52I CGGCCG 1 cut(s) 1125
Eco57I CTGAAG 1 cut(s) 1193
Eco88I CYCGRG 2 cut(s) 481, 646
EcoRII CCWGG 1 cut(s) 1018
FaeI CATG 6 cut(s) 81, 124, 144, 328, 622, 1156
FalI AAGNNNNNCTT 2 cut(s) 879, 911
FaqI GGGAC 1 cut(s) 470
FatI CATG 6 cut(s) 77, 120, 140, 324, 618, 1152
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 4 cut(s) 493, 604, 828, 1125
FokI GGATG 5 cut(s) 75, 194, 320, 1105, 1270
Fsp4HI GCNGC 4 cut(s) 493, 604, 828, 1125
FspBI CTAG 5 cut(s) 284, 345, 863, 915, 936
FspI TGCGCA 1 cut(s) 244
GlaI GCGC 4 cut(s) 244, 658, 751, 1098
GluI GCNGC 4 cut(s) 493, 604, 828, 1125
GsuI CTGGAG 1 cut(s) 96
HaeII RGCGCY 1 cut(s) 1100
HaeIII GGCC 3 cut(s) 92, 264, 1127
HapII CCGG 2 cut(s) 1105, 1128
HhaI GCGC 4 cut(s) 245, 659, 752, 1099
Hin1II CATG 6 cut(s) 81, 124, 144, 328, 622, 1156
Hin6I GCGC 4 cut(s) 243, 657, 750, 1097
HinP1I GCGC 4 cut(s) 243, 657, 750, 1097
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 7 cut(s) 137, 169, 439, 578, 726, 982, 1189
HpaII CCGG 2 cut(s) 1105, 1128
HphI GGTGA 2 cut(s) 1007, 1094
Hpy166II GTNNAC 4 cut(s) 517, 669, 775, 788
Hpy188I TCNGA 3 cut(s) 62, 1112, 1194
Hpy188III TCNNGA 8 cut(s) 113, 199, 466, 483, 648, 670, 988, 1089
Hpy8I GTNNAC 4 cut(s) 517, 669, 775, 788
Hpy99I CGWCG 2 cut(s) 430, 749
HpyAV CCTTC 2 cut(s) 254, 1171
HpyCH4III ACNGT 2 cut(s) 635, 761
HpyCH4IV ACGT 2 cut(s) 8, 519
HpyCH4V TGCA 5 cut(s) 278, 413, 495, 1224, 1283
HpyF10VI GCNNNNNNNGC 1 cut(s) 334
HpyF3I CTNAG 6 cut(s) 128, 583, 834, 896, 966, 1064
HpySE526I ACGT 2 cut(s) 8, 519
Hsp92II CATG 6 cut(s) 81, 124, 144, 328, 622, 1156
HspAI GCGC 4 cut(s) 243, 657, 750, 1097
KpnI GGTACC 1 cut(s) 904
KspI CCGCGG 1 cut(s) 1125
LguI GCTCTTC 2 cut(s) 1021, 1046
LmnI GCTCC 3 cut(s) 592, 1060, 1208
Lsp1109I GCAGC 2 cut(s) 479, 839
LweI GCATC 3 cut(s) 321, 612, 651
MaeI CTAG 5 cut(s) 284, 345, 863, 915, 936
MaeII ACGT 2 cut(s) 8, 519
MaeIII GTNAC 4 cut(s) 51, 366, 443, 1067
MbiI CCGCTC 1 cut(s) 645
MboII GAAGA 7 cut(s) 281, 299, 310, 631, 1038, 1063, 1186
MhlI GDGCHC 1 cut(s) 1005
MluCI AATT 4 cut(s) 214, 357, 372, 707
MlyI GAGTC 1 cut(s) 131
MseI TTAA 4 cut(s) 710, 821, 921, 942
MslI CAYNNNNRTG 1 cut(s) 1020
MspA1I CMGCKG 1 cut(s) 1124
MspI CCGG 2 cut(s) 1105, 1128
MspR9I CCNGG 1 cut(s) 1020
Mva1269I GAATGC 1 cut(s) 562
MvaI CCWGG 1 cut(s) 1020
MvnI CGCG 5 cut(s) 299, 430, 554, 659, 1124
MwoI GCNNNNNNNGC 1 cut(s) 334
NlaIII CATG 6 cut(s) 81, 124, 144, 328, 622, 1156
NlaIV GGNNCC 1 cut(s) 902
NmeAIII GCCGAG 1 cut(s) 290
NmuCI GTSAC 2 cut(s) 366, 1067
NsbI TGCGCA 1 cut(s) 244
NspI RCATGY 1 cut(s) 328
OliI CACNNNNGTG 1 cut(s) 1020
PciSI GCTCTTC 2 cut(s) 1021, 1046
PctI GAATGC 1 cut(s) 562
PfeI GAWTC 6 cut(s) 169, 439, 578, 726, 982, 1189
PinAI ACCGGT 1 cut(s) 1104
PkrI GCNGC 4 cut(s) 494, 605, 829, 1126
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 1018
PspGI CCWGG 1 cut(s) 1018
PspN4I GGNNCC 1 cut(s) 902
PstI CTGCAG 1 cut(s) 497
RsaI GTAC 5 cut(s) 525, 723, 768, 902, 1139
RsaNI GTAC 5 cut(s) 524, 722, 767, 901, 1138
RseI CAYNNNNRTG 1 cut(s) 1020
SacII CCGCGG 1 cut(s) 1125
SapI GCTCTTC 2 cut(s) 1021, 1046
SaqAI TTAA 4 cut(s) 710, 821, 921, 942
SatI GCNGC 4 cut(s) 493, 604, 828, 1125
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 1020
SduI GDGCHC 1 cut(s) 1005
SfaNI GCATC 3 cut(s) 321, 612, 651
SfcI CTRYAG 1 cut(s) 493
Sfr303I CCGCGG 1 cut(s) 1125
SgrBI CCGCGG 1 cut(s) 1125
SmiMI CAYNNNNRTG 1 cut(s) 1020
SmlI CTYRAG 1 cut(s) 986
SmoI CTYRAG 1 cut(s) 986
SpeI ACTAGT 1 cut(s) 935
Sse9I AATT 4 cut(s) 214, 357, 372, 707
SsiI CCGC 8 cut(s) 40, 160, 299, 603, 643, 802, 1122, 1124
SspI AATATT 1 cut(s) 1231
SspMI CTAG 5 cut(s) 284, 345, 863, 915, 936
StyD4I CCNGG 1 cut(s) 1018
TaaI ACNGT 2 cut(s) 635, 761
TaiI ACGT 2 cut(s) 11, 522
TaqI TCGA 6 cut(s) 258, 321, 398, 740, 793, 1080
TasI AATT 4 cut(s) 214, 357, 372, 707
TatI WGTACW 1 cut(s) 523
TauI GCSGC 2 cut(s) 606, 1127
TfiI GAWTC 6 cut(s) 169, 439, 578, 726, 982, 1189
Tru1I TTAA 4 cut(s) 710, 821, 921, 942
Tru9I TTAA 4 cut(s) 710, 821, 921, 942
TscAI CASTG 3 cut(s) 139, 502, 739
TseFI GTSAC 2 cut(s) 366, 1067
TseI GCWGC 2 cut(s) 492, 827
Tsp45I GTSAC 2 cut(s) 366, 1067
TspDTI ATGAA 2 cut(s) 137, 306
TspGWI ACGGA 2 cut(s) 354, 590
TspRI CASTG 3 cut(s) 139, 502, 739
XceI RCATGY 1 cut(s) 328
XmiI GTMKAC 1 cut(s) 774
XspI CTAG 5 cut(s) 284, 345, 863, 915, 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.