Rh1BG291000

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0003355
Physical Location & Seq
Reverse (-)
42706603 .. 42709122
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG291000.1

Sequence Viewer

Length: 639 bp
ATGGGCTGCAGTGCAAGTCTAGTTGCCATTGATGTTATGCAGAATTTGTTCAAGTCATACAAGAATTCATATGCTGTTGTTGTCAGCACTGAATCCATGGCTCCTAATTGGTATTGTGGGAAAGAAAAATCCATGATGCTCACGAACTGTTTGTTTCGGGCAGGAGGGTGTTCGATGCTGTTCACAAATAGAAGATCCCTTCAGCACCTAGCAATGTTGAAATTGAAGTGGTTGGTCAGAACCCATATTGGCTCAAGTGACAAGGCTTATGAATGTTGCATACAACTTGAAGATGAAAGTGGACATCTAGGCTTTCGACTTACCAAACACCTCACAGAAGCAACTGCTCTTGCTTTCACAGTGAACCTCCAAGTTTTAGTGCCAAAAGTGCTTCCACTAAGAGAAATAATCCGATACCTGGTGGCTTCTAGGCTTCAGAAAAACAGCAGTACCAAATGCCAAAATCTAGAAGCGGTTGGAGTTGGTTTGAATATAAAGGCTGGAATTCAACACTTTTGTCTCCACCCTGGTGGAAGAGCAATTATAAATGGGATAGTTAACTCCAATTGCATGAAGCCTGTGAATGGGATACAAGACAAGGATGTGAATGCCATAGAGGAGTATGAGCTCGCGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

212

Amino Acids

23.5

Weight (kDa)

9.06

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 1 - 142 3e-56 FAE1/Type III polyketide synthase-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 545
AccII CGCG 1 cut(s) 632
AciI CCGC 1 cut(s) 473
AclWI GGATC 1 cut(s) 189
AcsI RAATTY 3 cut(s) 43, 64, 504
AcuI CTGAAG 2 cut(s) 185, 419
AfaI GTAC 1 cut(s) 451
AfiI CCNNNNNNNGG 2 cut(s) 418, 584
AgsI TTSAA 6 cut(s) 52, 220, 226, 290, 490, 509
AjnI CCWGG 2 cut(s) 417, 526
AleI CACNNNNGTG 1 cut(s) 528
AluBI AGCT 1 cut(s) 628
AluI AGCT 1 cut(s) 628
Alw21I GWGCWC 1 cut(s) 630
Alw26I GTCTC 1 cut(s) 524
AlwI GGATC 1 cut(s) 189
ApeKI GCWGC 1 cut(s) 6
ApoI RAATTY 3 cut(s) 43, 64, 504
Asp700I GAANNNNTTC 1 cut(s) 47
BaeI ACNNNNGTAYC 2 cut(s) 433, 466
BanII GRGCYC 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 630
BciT130I CCWGG 2 cut(s) 419, 528
BciVI GTATCC 1 cut(s) 582
BcoDI GTCTC 1 cut(s) 524
BfaI CTAG 5 cut(s) 20, 209, 308, 429, 467
BfmI CTRYAG 1 cut(s) 7
BfuI GTATCC 1 cut(s) 582
BisI GCNGC 1 cut(s) 7
BlsI GCNGC 1 cut(s) 8
Bme1390I CCNGG 2 cut(s) 419, 528
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 2 cut(s) 419, 528
BmsI GCATC 2 cut(s) 126, 165
BpuEI CTTGAG 1 cut(s) 238
BsaJI CCNNGG 2 cut(s) 96, 526
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 584
Bse3DI GCAATG 1 cut(s) 219
BseBI CCWGG 2 cut(s) 419, 528
BseDI CCNNGG 2 cut(s) 96, 526
BseGI GGATG 1 cut(s) 607
BseLI CCNNNNNNNGG 2 cut(s) 418, 584
BseMI GCAATG 1 cut(s) 219
BseRI GAGGAG 1 cut(s) 632
Bsh1236I CGCG 1 cut(s) 632
BsiHKAI GWGCWC 1 cut(s) 630
BslI CCNNNNNNNGG 2 cut(s) 418, 584
BsmAI GTCTC 1 cut(s) 524
BsmI GAATGC 1 cut(s) 613
Bsp1286I GDGCHC 1 cut(s) 630
Bsp143I GATC 1 cut(s) 194
Bsp19I CCATGG 1 cut(s) 96
Bsp68I TCGCGA 1 cut(s) 632
BspACI CCGC 1 cut(s) 473
BspFNI CGCG 1 cut(s) 632
BspLI GGNNCC 1 cut(s) 102
BspMAI CTGCAG 1 cut(s) 11
BspPI GGATC 1 cut(s) 189
BspQI GCTCTTC 1 cut(s) 529
BsrDI GCAATG 1 cut(s) 219
BssECI CCNNGG 2 cut(s) 96, 526
BssMI GATC 1 cut(s) 194
BssT1I CCWWGG 1 cut(s) 96
Bst2UI CCWGG 2 cut(s) 419, 528
Bst4CI ACNGT 2 cut(s) 149, 361
Bst6I CTCTTC 1 cut(s) 529
BstC8I GCNNGC 1 cut(s) 630
BstDEI CTNAG 1 cut(s) 398
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 607
BstFNI CGCG 1 cut(s) 632
BstKTI GATC 1 cut(s) 197
BstMAI GTCTC 1 cut(s) 524
BstMBI GATC 1 cut(s) 194
BstMWI GCNNNNNNNGC 1 cut(s) 388
BstNI CCWGG 2 cut(s) 419, 528
BstSCI CCNGG 2 cut(s) 417, 526
BstSFI CTRYAG 1 cut(s) 7
BstUI CGCG 1 cut(s) 632
BstX2I RGATCY 1 cut(s) 194
BstXI CCANNNNNNTGG 1 cut(s) 530
BstYI RGATCY 1 cut(s) 194
BsuI GTATCC 1 cut(s) 582
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 607
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 3 cut(s) 16, 87, 366
BtuMI TCGCGA 1 cut(s) 632
Cac8I GCNNGC 1 cut(s) 630
CsiI ACCWGGT 1 cut(s) 417
Csp6I GTAC 1 cut(s) 450
CviAII CATG 3 cut(s) 97, 133, 571
CviQI GTAC 1 cut(s) 450
DdeI CTNAG 1 cut(s) 398
DpnI GATC 1 cut(s) 196
DpnII GATC 1 cut(s) 194
Eam1104I CTCTTC 1 cut(s) 529
EarI CTCTTC 1 cut(s) 529
Ecl136II GAGCTC 1 cut(s) 628
Eco130I CCWWGG 1 cut(s) 96
Eco24I GRGCYC 1 cut(s) 630
Eco53kI GAGCTC 1 cut(s) 628
Eco57I CTGAAG 2 cut(s) 185, 419
EcoICRI GAGCTC 1 cut(s) 628
EcoRI GAATTC 2 cut(s) 64, 504
EcoRII CCWGG 2 cut(s) 417, 526
EcoT14I CCWWGG 1 cut(s) 96
EcoT38I GRGCYC 1 cut(s) 630
ErhI CCWWGG 1 cut(s) 96
FaeI CATG 3 cut(s) 100, 136, 574
FatI CATG 3 cut(s) 96, 132, 570
FauNDI CATATG 1 cut(s) 70
Fnu4HI GCNGC 1 cut(s) 7
FokI GGATG 1 cut(s) 614
FriOI GRGCYC 1 cut(s) 630
Fsp4HI GCNGC 1 cut(s) 7
FspBI CTAG 5 cut(s) 20, 209, 308, 429, 467
GluI GCNGC 1 cut(s) 7
Hin1II CATG 3 cut(s) 100, 136, 574
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 1 cut(s) 92
HpaI GTTAAC 1 cut(s) 559
Hpy166II GTNNAC 4 cut(s) 183, 302, 364, 559
Hpy188I TCNGA 3 cut(s) 239, 413, 438
Hpy188III TCNNGA 3 cut(s) 142, 467, 631
Hpy8I GTNNAC 4 cut(s) 183, 302, 364, 559
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 2 cut(s) 149, 361
HpyCH4V TGCA 5 cut(s) 9, 14, 40, 279, 570
HpyF10VI GCNNNNNNNGC 1 cut(s) 388
HpyF3I CTNAG 1 cut(s) 398
Hsp92II CATG 3 cut(s) 100, 136, 574
KspAI GTTAAC 1 cut(s) 559
Kzo9I GATC 1 cut(s) 194
LguI GCTCTTC 1 cut(s) 529
LmnI GCTCC 1 cut(s) 106
LpnPI CCDG 7 cut(s) 147, 404, 431, 486, 513, 540, 591
LweI GCATC 2 cut(s) 126, 165
MabI ACCWGGT 1 cut(s) 417
MaeI CTAG 5 cut(s) 20, 209, 308, 429, 467
MaeIII GTNAC 1 cut(s) 257
MalI GATC 1 cut(s) 196
MboI GATC 1 cut(s) 194
MboII GAAGA 3 cut(s) 204, 302, 546
MfeI CAATTG 1 cut(s) 565
MflI RGATCY 1 cut(s) 194
MhlI GDGCHC 1 cut(s) 630
MluCI AATT 7 cut(s) 43, 64, 106, 221, 504, 540, 565
MmeI TCCRAC 1 cut(s) 457
MnlI CCTC 4 cut(s) 158, 341, 377, 610
MroXI GAANNNNTTC 1 cut(s) 47
MseI TTAA 1 cut(s) 558
MslI CAYNNNNRTG 1 cut(s) 528
MspR9I CCNGG 2 cut(s) 419, 528
MunI CAATTG 1 cut(s) 565
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 2 cut(s) 419, 528
MvnI CGCG 1 cut(s) 632
MwoI GCNNNNNNNGC 1 cut(s) 388
NcoI CCATGG 1 cut(s) 96
NdeI CATATG 1 cut(s) 70
NdeII GATC 1 cut(s) 194
NlaIII CATG 3 cut(s) 100, 136, 574
NlaIV GGNNCC 1 cut(s) 102
NmuCI GTSAC 1 cut(s) 257
NruI TCGCGA 1 cut(s) 632
OliI CACNNNNGTG 1 cut(s) 528
PciSI GCTCTTC 1 cut(s) 529
PctI GAATGC 1 cut(s) 613
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 1 cut(s) 92
PkrI GCNGC 1 cut(s) 8
PsiI TTATAA 1 cut(s) 545
Psp124BI GAGCTC 1 cut(s) 630
Psp6I CCWGG 2 cut(s) 417, 526
PspGI CCWGG 2 cut(s) 417, 526
PspN4I GGNNCC 1 cut(s) 102
PstI CTGCAG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 194
RruI TCGCGA 1 cut(s) 632
RsaI GTAC 1 cut(s) 451
RsaNI GTAC 1 cut(s) 450
RseI CAYNNNNRTG 1 cut(s) 528
SacI GAGCTC 1 cut(s) 630
SapI GCTCTTC 1 cut(s) 529
SaqAI TTAA 1 cut(s) 558
SatI GCNGC 1 cut(s) 7
Sau3AI GATC 1 cut(s) 194
ScrFI CCNGG 2 cut(s) 419, 528
SduI GDGCHC 1 cut(s) 630
SetI ASST 5 cut(s) 210, 333, 369, 420, 630
SexAI ACCWGGT 1 cut(s) 417
SfaNI GCATC 2 cut(s) 126, 165
SfcI CTRYAG 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 528
SmlI CTYRAG 1 cut(s) 253
SmoI CTYRAG 1 cut(s) 253
Sse9I AATT 7 cut(s) 43, 64, 106, 221, 504, 540, 565
SsiI CCGC 1 cut(s) 473
SspMI CTAG 5 cut(s) 20, 209, 308, 429, 467
SstI GAGCTC 1 cut(s) 630
StyD4I CCNGG 2 cut(s) 417, 526
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 2 cut(s) 149, 361
TaqI TCGA 2 cut(s) 173, 316
TasI AATT 7 cut(s) 43, 64, 106, 221, 504, 540, 565
TfiI GAWTC 1 cut(s) 92
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TscAI CASTG 3 cut(s) 16, 94, 366
TseFI GTSAC 1 cut(s) 257
TseI GCWGC 1 cut(s) 6
Tsp45I GTSAC 1 cut(s) 257
TspDTI ATGAA 4 cut(s) 57, 285, 309, 587
TspRI CASTG 3 cut(s) 16, 94, 366
XapI RAATTY 3 cut(s) 43, 64, 504
XbaI TCTAGA 1 cut(s) 466
XmnI GAANNNNTTC 1 cut(s) 47
XspI CTAG 5 cut(s) 20, 209, 308, 429, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.