MD07G1187400.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003355
Physical Location & Seq
Forward (+)
26827200 .. 26828615
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1187400.v1.1.491

Sequence Viewer

Length: 1416 bp
ATGGGGATCCTGATGGCAACGTGTTTGCTGGCTCTTTTCTATGGTTTCTCCTGCCTCTGTAAGTTACTTCTCAGAAGGAGAGACCAGGCATGCTATCTGCTGGCCTACGAGTGCCACATGCCACCAGACGACCTGAAGCTCAGCACTGATTCATGCGTGAAAATCGTCTTGCGGAACAGGAATCTAGGACTGGAAGAATTCAGGTTTCTCTTGAAAACTATTACCCATTCGGGCATTGGCGAGGAAACTTACTGCGCAAGAAACATCATCCAAGGCCGAGAAGAAACTGCAACCCTAGAAGATGAACTTGCGGAGATGGATGGCATCATCTTTGACGTGCTGGACAAGCTTTTCGCTCGGGCTACCTCAATTTCTCCGTCGCAAATCGACATTCTTGTCGTCAATGTGTCGATGTTCTCCCCTGCACCCTCCCTATCTTCACGTATAGTGAATCGTTACAAGATGAGGGACGACATCAAGAGCTTCAACCTCTCGGGAATGGGCTGCAGTGCGACCCTCATTGCCATTGACGTTGTACAAAACTTGTTCAAGTCTCACAAGAATGCGAATGCCATCGTTGTAAGCACGGAATCGTTGGCTCCTCATTGGTATTGCGGCGTAGAAAAATCCATGATGCTCACAAACTGTCTCTTCCGCTCGGGAGGGTGCGCGATGCTGTTCACGAACAACCGAAGCCTAAAGCACCAAGCCAAGCTGAAATTAAATCATTTGGTACGAATGCACACCGGCTCGAACGAAGAAGCATATAACTGTTGCATACAGGTAGAAGATGAGAGTGGAAATCGAGGTTTCCGCCTTACCAAATACCTAGTAAAAGCAGCAACTCTGGGTTTTACAATGAACCTCCAAGTTCTAGCACCAAAAATGCTTCCACTAAGAGAAATACTTAGGTATCTGGTGGCCTCTAGGCTTAACAATATTAGAACTAGTATTACAAGCCAAAAGCCAAAAGCAGCTGGGGAACTAGGGTTGAATCTCAAGACAGGAATAGAGCACTTCTGTATTCACCCTGGTGGAAGAGCAATCATAGATGGGATGGAGAAGAGCTTAGGGCTAAGTGATTATGACGTTGAGCCGTCAAGAATGGCGCTTCACCGGTTCGGAAACACATCGGCGGCCGGATTTTGGTACGCTTTGGGATACATGGAGGCAAAGAAGAGGCTTAAGAAGGGGAACAGGATTCTGATGACTGGATTTGGAGCTGGTTTTAAGTGCAACAATATTGTGTGGGAAGTGATGAAGGACTTGGATGATGTCAATGCGTGGAAAGACTGCATAGATAGTTACCCTAGGGATCAAAACCAAGTCAATCCGTTCATGGAGAAGTATGGCTGGATCAATGATGATTATCTAGGCCTTGTTAGGTTTGACTTTTCCCTATTTGCTATTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

472

Amino Acids

53.05

Weight (kDa)

8.49

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 25 - 308 1.2e-113 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 336 - 418 9.4e-13 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 339 - 412 7.3e-10 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
Acc16I TGCGCA 1 cut(s) 256
AccBSI CCGCTC 1 cut(s) 657
AccII CGCG 1 cut(s) 671
AciI CCGC 6 cut(s) 172, 311, 615, 655, 814, 1136
AclWI GGATC 3 cut(s) 14, 1323, 1364
AcoI YGGCCR 1 cut(s) 1137
AcsI RAATTY 1 cut(s) 197
AcuI CTGAAG 1 cut(s) 155
AfaI GTAC 3 cut(s) 537, 735, 1151
AfiI CCNNNNNNNGG 1 cut(s) 1146
AflII CTTAAG 1 cut(s) 1184
AflIII ACRYGT 1 cut(s) 20
AgeI ACCGGT 1 cut(s) 1116
AgsI TTSAA 4 cut(s) 214, 487, 550, 994
AhlI ACTAGT 1 cut(s) 947
AjiI CACGTC 1 cut(s) 337
AjnI CCWGG 2 cut(s) 84, 1030
AleI CACNNNNGTG 1 cut(s) 1032
AluBI AGCT 7 cut(s) 139, 349, 483, 715, 977, 1068, 1223
AluI AGCT 7 cut(s) 139, 349, 483, 715, 977, 1068, 1223
Alw21I GWGCWC 1 cut(s) 1017
Alw26I GTCTC 3 cut(s) 75, 558, 653
AlwI GGATC 3 cut(s) 14, 1323, 1364
Ama87I CYCGRG 3 cut(s) 357, 493, 658
AoxI GGCC 5 cut(s) 102, 274, 921, 1137, 1375
ApeKI GCWGC 3 cut(s) 504, 839, 974
ApoI RAATTY 1 cut(s) 197
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AsiGI ACCGGT 1 cut(s) 1116
AspA2I CCTAGG 1 cut(s) 1310
AspLEI GCGC 3 cut(s) 257, 671, 1111
AsuHPI GGTGA 2 cut(s) 1019, 1106
AvaI CYCGRG 3 cut(s) 357, 493, 658
AvrII CCTAGG 1 cut(s) 1310
BaeI ACNNNNGTAYC 2 cut(s) 896, 929
BamHI GGATCC 1 cut(s) 6
Bbv12I GWGCWC 1 cut(s) 1017
BbvI GCAGC 3 cut(s) 491, 851, 986
BccI CCATC 6 cut(s) 7, 310, 314, 581, 1046, 1051
BceAI ACGGC 1 cut(s) 1081
BciT130I CCWGG 2 cut(s) 86, 1032
BciVI GTATCC 1 cut(s) 1154
BcoDI GTCTC 3 cut(s) 75, 558, 653
BcuI ACTAGT 1 cut(s) 947
BfaI CTAG 9 cut(s) 185, 296, 830, 875, 927, 948, 986, 1311, 1373
BfmI CTRYAG 1 cut(s) 505
BfoI RGCGCY 1 cut(s) 1112
BfrI CTTAAG 1 cut(s) 1184
BfuI GTATCC 1 cut(s) 1154
BisI GCNGC 5 cut(s) 505, 616, 840, 975, 1137
BlnI CCTAGG 1 cut(s) 1310
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 5 cut(s) 506, 617, 841, 976, 1138
Bme1390I CCNGG 2 cut(s) 86, 1032
BmeT110I CYCGRG 3 cut(s) 357, 493, 658
BmgBI CACGTC 1 cut(s) 337
BmiI GGNNCC 2 cut(s) 8, 600
BmrFI CCNGG 2 cut(s) 86, 1032
BmsI GCATC 3 cut(s) 333, 624, 663
Bpu10I CCTNAGC 1 cut(s) 1069
Bpu1102I GCTNAGC 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 983
BsaAI YACGTR 1 cut(s) 443
BsaBI GATNNNNATC 1 cut(s) 1368
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 271, 1030, 1310
BsaWI WCCGGW 1 cut(s) 1116
Bsc4I CCNNNNNNNGG 1 cut(s) 1146
Bse118I RCCGGY 2 cut(s) 746, 1116
Bse1I ACTGG 2 cut(s) 195, 1216
Bse3DI GCAATG 1 cut(s) 519
Bse8I GATNNNNATC 1 cut(s) 1368
BseBI CCWGG 2 cut(s) 86, 1032
BseDI CCNNGG 3 cut(s) 271, 1030, 1310
BseGI GGATG 4 cut(s) 267, 325, 1062, 1276
BseJI GATNNNNATC 1 cut(s) 1368
BseLI CCNNNNNNNGG 1 cut(s) 1146
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 2 cut(s) 85, 154
BseNI ACTGG 2 cut(s) 195, 1216
BseRI GAGGAG 1 cut(s) 591
BseX3I CGGCCG 1 cut(s) 1137
BseXI GCAGC 3 cut(s) 491, 851, 986
BseYI CCCAGC 1 cut(s) 977
BsgI GTGCAG 1 cut(s) 408
Bsh1236I CGCG 1 cut(s) 671
Bsh1285I CGRYCG 1 cut(s) 1140
BshFI GGCC 5 cut(s) 104, 276, 923, 1139, 1377
BshTI ACCGGT 1 cut(s) 1116
BsiEI CGRYCG 1 cut(s) 1140
BsiHKAI GWGCWC 1 cut(s) 1017
BsiHKCI CYCGRG 3 cut(s) 357, 493, 658
BsiSI CCGG 3 cut(s) 747, 1117, 1140
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 1 cut(s) 1146
BsmAI GTCTC 3 cut(s) 75, 558, 653
BsmFI GGGAC 1 cut(s) 482
BsmI GAATGC 3 cut(s) 568, 574, 744
BsnI GGCC 5 cut(s) 104, 276, 923, 1139, 1377
Bso31I GGTCTC 1 cut(s) 75
BsoBI CYCGRG 3 cut(s) 357, 493, 658
Bsp1286I GDGCHC 1 cut(s) 1017
Bsp1407I TGTACA 1 cut(s) 535
Bsp143I GATC 3 cut(s) 6, 1315, 1356
Bsp1720I GCTNAGC 1 cut(s) 140
BspACI CCGC 6 cut(s) 172, 311, 615, 655, 814, 1136
BspANI GGCC 5 cut(s) 104, 276, 923, 1139, 1377
BspCNI CTCAG 2 cut(s) 84, 153
BspFNI CGCG 1 cut(s) 671
BspLI GGNNCC 2 cut(s) 8, 600
BspMAI CTGCAG 1 cut(s) 509
BspPI GGATC 3 cut(s) 14, 1323, 1364
BspQI GCTCTTC 2 cut(s) 1033, 1058
BspTI CTTAAG 1 cut(s) 1184
BspTNI GGTCTC 1 cut(s) 75
BsrBI CCGCTC 1 cut(s) 657
BsrDI GCAATG 1 cut(s) 519
BsrFI RCCGGY 2 cut(s) 746, 1116
BsrGI TGTACA 1 cut(s) 535
BsrI ACTGG 2 cut(s) 195, 1216
BssAI RCCGGY 2 cut(s) 746, 1116
BssECI CCNNGG 3 cut(s) 271, 1030, 1310
BssMI GATC 3 cut(s) 6, 1315, 1356
BssT1I CCWWGG 2 cut(s) 271, 1310
Bst2UI CCWGG 2 cut(s) 86, 1032
Bst4CI ACNGT 2 cut(s) 647, 773
Bst6I CTCTTC 4 cut(s) 656, 1033, 1058, 1172
BstAFI CTTAAG 1 cut(s) 1184
BstAUI TGTACA 1 cut(s) 535
BstBAI YACGTR 1 cut(s) 443
BstC8I GCNNGC 3 cut(s) 30, 91, 102
BstDEI CTNAG 6 cut(s) 71, 140, 896, 908, 1069, 1076
BstF5I GGATG 4 cut(s) 267, 325, 1062, 1276
BstFNI CGCG 1 cut(s) 671
BstH2I RGCGCY 1 cut(s) 1112
BstHHI GCGC 3 cut(s) 257, 671, 1111
BstKTI GATC 3 cut(s) 9, 1318, 1359
BstMAI GTCTC 3 cut(s) 75, 558, 653
BstMBI GATC 3 cut(s) 6, 1315, 1356
BstMCI CGRYCG 1 cut(s) 1140
BstMWI GCNNNNNNNGC 1 cut(s) 346
BstNI CCWGG 2 cut(s) 86, 1032
BstNSI RCATGY 2 cut(s) 93, 121
BstSCI CCNGG 2 cut(s) 84, 1030
BstSFI CTRYAG 1 cut(s) 505
BstUI CGCG 1 cut(s) 671
BstV1I GCAGC 3 cut(s) 491, 851, 986
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BstZI CGGCCG 1 cut(s) 1137
BsuI GTATCC 1 cut(s) 1154
BsuRI GGCC 5 cut(s) 104, 276, 923, 1139, 1377
BtgZI GCGATG 1 cut(s) 686
BtrI CACGTC 1 cut(s) 337
BtsCI GGATG 4 cut(s) 267, 325, 1062, 1276
BtsI GCAGTG 1 cut(s) 514
BtsIMutI CAGTG 2 cut(s) 144, 514
Cac8I GCNNGC 3 cut(s) 30, 91, 102
CfoI GCGC 3 cut(s) 257, 671, 1111
Cfr10I RCCGGY 2 cut(s) 746, 1116
Csp6I GTAC 3 cut(s) 536, 734, 1150
CspAI ACCGGT 1 cut(s) 1116
CviAII CATG 6 cut(s) 90, 118, 153, 631, 1165, 1339
CviQI GTAC 3 cut(s) 536, 734, 1150
DdeI CTNAG 6 cut(s) 71, 140, 896, 908, 1069, 1076
DpnI GATC 3 cut(s) 8, 1317, 1358
DpnII GATC 3 cut(s) 6, 1315, 1356
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
EaeI YGGCCR 1 cut(s) 1137
EagI CGGCCG 1 cut(s) 1137
Eam1104I CTCTTC 4 cut(s) 656, 1033, 1058, 1172
EarI CTCTTC 4 cut(s) 656, 1033, 1058, 1172
EciI GGCGGA 1 cut(s) 803
EclXI CGGCCG 1 cut(s) 1137
Eco130I CCWWGG 2 cut(s) 271, 1310
Eco147I AGGCCT 1 cut(s) 1377
Eco31I GGTCTC 1 cut(s) 75
Eco52I CGGCCG 1 cut(s) 1137
Eco57I CTGAAG 1 cut(s) 155
Eco88I CYCGRG 3 cut(s) 357, 493, 658
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 2 cut(s) 84, 1030
EcoT14I CCWWGG 2 cut(s) 271, 1310
ErhI CCWWGG 2 cut(s) 271, 1310
FaeI CATG 6 cut(s) 93, 121, 156, 634, 1168, 1342
FalI AAGNNNNNCTT 2 cut(s) 291, 323
FaqI GGGAC 1 cut(s) 482
FatI CATG 6 cut(s) 89, 117, 152, 630, 1164, 1338
Fnu4HI GCNGC 5 cut(s) 505, 616, 840, 975, 1137
FokI GGATG 4 cut(s) 254, 332, 1069, 1283
Fsp4HI GCNGC 5 cut(s) 505, 616, 840, 975, 1137
FspBI CTAG 9 cut(s) 185, 296, 830, 875, 927, 948, 986, 1311, 1373
FspI TGCGCA 1 cut(s) 256
GlaI GCGC 3 cut(s) 256, 670, 1110
GluI GCNGC 5 cut(s) 505, 616, 840, 975, 1137
GsaI CCCAGC 1 cut(s) 981
HaeII RGCGCY 1 cut(s) 1112
HaeIII GGCC 5 cut(s) 104, 276, 923, 1139, 1377
HapII CCGG 3 cut(s) 747, 1117, 1140
HhaI GCGC 3 cut(s) 257, 671, 1111
Hin1II CATG 6 cut(s) 93, 121, 156, 634, 1168, 1342
Hin6I GCGC 3 cut(s) 255, 669, 1109
HinP1I GCGC 3 cut(s) 255, 669, 1109
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 6 cut(s) 149, 181, 451, 590, 994, 1201
HpaII CCGG 3 cut(s) 747, 1117, 1140
HphI GGTGA 2 cut(s) 1019, 1106
Hpy166II GTNNAC 1 cut(s) 681
Hpy188I TCNGA 3 cut(s) 74, 1124, 1206
Hpy188III TCNNGA 8 cut(s) 10, 211, 478, 495, 660, 682, 1000, 1101
Hpy8I GTNNAC 1 cut(s) 681
Hpy99I CGWCG 1 cut(s) 382
HpyAV CCTTC 3 cut(s) 69, 1183, 1255
HpyCH4III ACNGT 2 cut(s) 647, 773
HpyCH4IV ACGT 5 cut(s) 20, 336, 442, 531, 1089
HpyCH4V TGCA 7 cut(s) 290, 425, 507, 742, 777, 1236, 1296
HpyF10VI GCNNNNNNNGC 1 cut(s) 346
HpyF3I CTNAG 6 cut(s) 71, 140, 896, 908, 1069, 1076
HpySE526I ACGT 5 cut(s) 20, 336, 442, 531, 1089
Hsp92II CATG 6 cut(s) 93, 121, 156, 634, 1168, 1342
HspAI GCGC 3 cut(s) 255, 669, 1109
Kzo9I GATC 3 cut(s) 6, 1315, 1356
LguI GCTCTTC 2 cut(s) 1033, 1058
LmnI GCTCC 2 cut(s) 604, 1220
Lsp1109I GCAGC 3 cut(s) 491, 851, 986
LweI GCATC 3 cut(s) 333, 624, 663
MaeI CTAG 9 cut(s) 185, 296, 830, 875, 927, 948, 986, 1311, 1373
MaeII ACGT 5 cut(s) 20, 336, 442, 531, 1089
MaeIII GTNAC 3 cut(s) 63, 455, 1304
MalI GATC 3 cut(s) 8, 1317, 1358
MbiI CCGCTC 1 cut(s) 657
MboI GATC 3 cut(s) 6, 1315, 1356
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 1 cut(s) 1017
MluCI AATT 3 cut(s) 197, 369, 719
MseI TTAA 4 cut(s) 722, 933, 1185, 1230
MslI CAYNNNNRTG 2 cut(s) 561, 1032
MspA1I CMGCKG 1 cut(s) 977
MspCI CTTAAG 1 cut(s) 1184
MspI CCGG 3 cut(s) 747, 1117, 1140
MspR9I CCNGG 2 cut(s) 86, 1032
Mva1269I GAATGC 3 cut(s) 568, 574, 744
MvaI CCWGG 2 cut(s) 86, 1032
MvnI CGCG 1 cut(s) 671
MwoI GCNNNNNNNGC 1 cut(s) 346
NdeII GATC 3 cut(s) 6, 1315, 1356
NlaIII CATG 6 cut(s) 93, 121, 156, 634, 1168, 1342
NlaIV GGNNCC 2 cut(s) 8, 600
NmeAIII GCCGAG 1 cut(s) 302
NsbI TGCGCA 1 cut(s) 256
NspI RCATGY 2 cut(s) 93, 121
OliI CACNNNNGTG 1 cut(s) 1032
PaeI GCATGC 1 cut(s) 93
PceI AGGCCT 1 cut(s) 1377
PciSI GCTCTTC 2 cut(s) 1033, 1058
PctI GAATGC 3 cut(s) 568, 574, 744
PfeI GAWTC 6 cut(s) 149, 181, 451, 590, 994, 1201
PinAI ACCGGT 1 cut(s) 1116
PkrI GCNGC 5 cut(s) 506, 617, 841, 976, 1138
Ppu21I YACGTR 1 cut(s) 443
Psp6I CCWGG 2 cut(s) 84, 1030
PspFI CCCAGC 1 cut(s) 977
PspGI CCWGG 2 cut(s) 84, 1030
PspN4I GGNNCC 2 cut(s) 8, 600
PsrI GAACNNNNNNTAC 2 cut(s) 937, 969
PstI CTGCAG 1 cut(s) 509
PsuI RGATCY 1 cut(s) 6
PvuII CAGCTG 1 cut(s) 977
RsaI GTAC 3 cut(s) 537, 735, 1151
RsaNI GTAC 3 cut(s) 536, 734, 1150
RseI CAYNNNNRTG 2 cut(s) 561, 1032
SapI GCTCTTC 2 cut(s) 1033, 1058
SaqAI TTAA 4 cut(s) 722, 933, 1185, 1230
SatI GCNGC 5 cut(s) 505, 616, 840, 975, 1137
Sau3AI GATC 3 cut(s) 6, 1315, 1356
ScrFI CCNGG 2 cut(s) 86, 1032
SduI GDGCHC 1 cut(s) 1017
SfaNI GCATC 3 cut(s) 333, 624, 663
SfcI CTRYAG 1 cut(s) 505
SmiMI CAYNNNNRTG 2 cut(s) 561, 1032
SmlI CTYRAG 2 cut(s) 998, 1184
SmoI CTYRAG 2 cut(s) 998, 1184
SpeI ACTAGT 1 cut(s) 947
SphI GCATGC 1 cut(s) 93
Sse9I AATT 3 cut(s) 197, 369, 719
SseBI AGGCCT 1 cut(s) 1377
SsiI CCGC 6 cut(s) 172, 311, 615, 655, 814, 1136
SspI AATATT 2 cut(s) 940, 1243
SspMI CTAG 9 cut(s) 185, 296, 830, 875, 927, 948, 986, 1311, 1373
StuI AGGCCT 1 cut(s) 1377
StyD4I CCNGG 2 cut(s) 84, 1030
StyI CCWWGG 2 cut(s) 271, 1310
TaaI ACNGT 2 cut(s) 647, 773
TaiI ACGT 5 cut(s) 23, 339, 445, 534, 1092
TaqI TCGA 4 cut(s) 387, 410, 752, 805
TasI AATT 3 cut(s) 197, 369, 719
TatI WGTACW 1 cut(s) 535
TauI GCSGC 2 cut(s) 618, 1139
TfiI GAWTC 6 cut(s) 149, 181, 451, 590, 994, 1201
Tru1I TTAA 4 cut(s) 722, 933, 1185, 1230
Tru9I TTAA 4 cut(s) 722, 933, 1185, 1230
TscAI CASTG 2 cut(s) 151, 514
TseI GCWGC 3 cut(s) 504, 839, 974
TspDTI ATGAA 5 cut(s) 141, 318, 875, 1274, 1327
TspGWI ACGGA 3 cut(s) 366, 602, 1323
TspRI CASTG 2 cut(s) 151, 514
Vha464I CTTAAG 1 cut(s) 1184
XapI RAATTY 1 cut(s) 197
XceI RCATGY 2 cut(s) 93, 121
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XmaJI CCTAGG 1 cut(s) 1310
XspI CTAG 9 cut(s) 185, 296, 830, 875, 927, 948, 986, 1311, 1373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.