MD07G1187800.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003355
Physical Location & Seq
Reverse (-)
26837140 .. 26838543
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1187800.v1.1.491

Sequence Viewer

Length: 1404 bp
ATGGAGATCCTCATGACAATGTCTCTACTCGTTCTTTTCGGCTTCTTCTCCTTCTTGAAGTTGGTTTTCCGAAGGGGAGATCAGTGCTGTTACCTGCTGGCTTACGAGTGCTACAAGGCGAAAGAAGACATGAAGATCAACAGCGATTCCTGCGCGAAGATCGTCTTGCGGAACAAGAATCTGGGGTTGGAGGAGTTCAGGTTTCTCTTGAAAACCATTGTCAATTCCGGTATTGGAGAAGAAACTTCCTGCCCAAGAAACATAATTGAAGGCCGAGAAGAAAATCCAACCAGGGCAGACGAGCTTAATGAGATGGATGGCATCATTTTCGACACGCTTGACAAGCTTTTCGCAAGGAATGCTGCAATTCCTCCTTCACAAATCGACATCCTTGTTGTCAATGTGTCGATGTTCTCCCCCGCACCCTCACTAACGTCGCGCATAATAAACCGGTACAAGATGAGGGAGGACATCAAGAGCTTCAACCTCTCGGGAATGGGCTGCAGCGCAAGCCTCGTTGCCATTGATGTTGTGCAGAACTTGTTCAAGTCGCATAAGAACGCAAATGCGGTTGTTGTCAGCACAGAATCCATAGCTCCAAACTGGTATTGCGGCAAAGAAAAATCCATGATGCTCACGAACTGTCTGTTCCGCTCGGGAGGTTGTTCGATGCTGTTCACAAATAACAGAACCCTACAGCACCAAGCCAAGTTGAAGTTACAGCATTTGGTACGTACCCATATTGGCTCGAACGCTGAAGCGTATGAATGCTGCATACAGCTAGAAGATGAGAGTGGATACCAAGGGTTTCGCCTCACCAAACACCTCACGAAAGCAGCTGCTATAGCTTTAACAATGAACCTCCAAGACCTAGTCCCAAAAGTGCTTCCACTGAGAGAATTATTTCACTACCTGATGGCCTCTAGGGTTCGGAATAGAACTGGTACTAAAAGCCAAAAGCTAGAAGCAGTTGGTATAGGGCTAAATCTCAAGGCAGGAATAGAGCATTTCTGCATTCACCCTGGTGGAAGAGCAATCATAGATGGAATTGGGAAGAGCTTAGGTCTAAGGGATTATGATCTTGAGCCGTCTAGAATGGCACTTCACCGGTTTGGCAACACGTCAGCAGCCGGATTTTGGTACGCTTTGGGATACATGGAGGCAAAGAAGAGGCTTAAGAAGGGCAACAAAATTCTGATGACTGGATTTGGAGCAGGTTTTAAGTGCAACAACATTGTGTGGGAAGTATTGAAGGACTTGGATGATGCCAATGTTTGGAAAGATTGCATAGAAAGTTACCCTCCTAATAATACTCTGGGCAATCCTTACATGGAGAAGTATGGTTGGCTCAACGACGAATATCTAAGCTTTGTTAGGCTCGATCTCTCCCAAATTTTCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

468

Amino Acids

52.61

Weight (kDa)

8.71

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 25 - 307 1.2e-113 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 333 - 415 1.6e-12 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 336 - 408 1.9e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 102, 1205
AccB7I CCANNNNNTGG 1 cut(s) 1275
AccBSI CCGCTC 1 cut(s) 654
AccII CGCG 2 cut(s) 155, 439
AciI CCGC 5 cut(s) 169, 420, 569, 612, 652
AcsI RAATTY 2 cut(s) 1191, 1392
AcuI CTGAAG 1 cut(s) 777
AfaI GTAC 5 cut(s) 455, 732, 736, 946, 1142
AfiI CCNNNNNNNGG 2 cut(s) 1137, 1275
AflII CTTAAG 1 cut(s) 1175
AflIII ACRYGT 1 cut(s) 1119
AgeI ACCGGT 2 cut(s) 450, 1107
AgsI TTSAA 7 cut(s) 58, 211, 269, 484, 547, 715, 1252
AjiI CACGTC 1 cut(s) 1122
AjnI CCWGG 2 cut(s) 290, 1021
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AleI CACNNNNGTG 1 cut(s) 1023
Alw26I GTCTC 1 cut(s) 27
Ama87I CYCGRG 2 cut(s) 490, 655
AoxI GGCC 2 cut(s) 271, 918
ApeKI GCWGC 7 cut(s) 362, 501, 504, 771, 836, 839, 1127
ApoI RAATTY 2 cut(s) 1191, 1392
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
AsiGI ACCGGT 2 cut(s) 450, 1107
Asp700I GAANNNNTTC 2 cut(s) 542, 903
AspLEI GCGC 3 cut(s) 155, 441, 509
AsuHPI GGTGA 3 cut(s) 808, 1010, 1097
AvaI CYCGRG 2 cut(s) 490, 655
BbsI GAAGAC 1 cut(s) 132
BbvI GCAGC 7 cut(s) 349, 488, 516, 758, 826, 848, 1139
BccI CCATC 4 cut(s) 307, 311, 910, 1037
BceAI ACGGC 1 cut(s) 1072
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 2 cut(s) 292, 1023
BciVI GTATCC 2 cut(s) 791, 1145
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 5 cut(s) 782, 872, 924, 962, 1092
BfmI CTRYAG 3 cut(s) 502, 695, 843
BfrI CTTAAG 1 cut(s) 1175
BfuAI ACCTGC 2 cut(s) 102, 1205
BfuI GTATCC 2 cut(s) 791, 1145
BisI GCNGC 8 cut(s) 363, 502, 505, 613, 772, 837, 840, 1128
BlsI GCNGC 8 cut(s) 364, 503, 506, 614, 773, 838, 841, 1129
Bme1390I CCNGG 2 cut(s) 292, 1023
BmeT110I CYCGRG 2 cut(s) 490, 655
BmgBI CACGTC 1 cut(s) 1122
BmrFI CCNGG 2 cut(s) 292, 1023
BmsI GCATC 4 cut(s) 330, 621, 660, 1255
BpiI GAAGAC 1 cut(s) 132
Bpu10I CCTNAGC 1 cut(s) 1060
BpuEI CTTGAG 2 cut(s) 974, 1103
BsaAI YACGTR 1 cut(s) 734
BsaBI GATNNNNATC 1 cut(s) 1077
BsaJI CCNNGG 3 cut(s) 291, 802, 1021
BsaWI WCCGGW 3 cut(s) 227, 450, 1107
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 2 cut(s) 1137, 1275
Bse118I RCCGGY 2 cut(s) 450, 1107
Bse1I ACTGG 3 cut(s) 608, 946, 1207
Bse8I GATNNNNATC 1 cut(s) 1077
BseBI CCWGG 2 cut(s) 292, 1023
BseDI CCNNGG 3 cut(s) 291, 802, 1021
BseGI GGATG 3 cut(s) 322, 387, 1267
BseJI GATNNNNATC 1 cut(s) 1077
BseLI CCNNNNNNNGG 2 cut(s) 1137, 1275
BseMII CTCAG 1 cut(s) 884
BseNI ACTGG 3 cut(s) 608, 946, 1207
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 7 cut(s) 349, 488, 516, 758, 826, 848, 1139
BsgI GTGCAG 1 cut(s) 554
Bsh1236I CGCG 2 cut(s) 155, 439
BshFI GGCC 2 cut(s) 273, 920
BshTI ACCGGT 2 cut(s) 450, 1107
BsiHKCI CYCGRG 2 cut(s) 490, 655
BsiSI CCGG 4 cut(s) 228, 451, 1108, 1131
BslFI GGGAC 1 cut(s) 860
BslI CCNNNNNNNGG 2 cut(s) 1137, 1275
BsmAI GTCTC 1 cut(s) 27
BsmFI GGGAC 1 cut(s) 860
BsmI GAATGC 3 cut(s) 364, 773, 1014
BsnI GGCC 2 cut(s) 273, 920
BsoBI CYCGRG 2 cut(s) 490, 655
Bsp143I GATC 6 cut(s) 6, 79, 135, 159, 1078, 1381
BspACI CCGC 5 cut(s) 169, 420, 569, 612, 652
BspANI GGCC 2 cut(s) 273, 920
BspCNI CTCAG 1 cut(s) 885
BspFNI CGCG 2 cut(s) 155, 439
BspHI TCATGA 1 cut(s) 12
BspMAI CTGCAG 1 cut(s) 506
BspMI ACCTGC 2 cut(s) 102, 1205
BspQI GCTCTTC 2 cut(s) 1024, 1049
BspTI CTTAAG 1 cut(s) 1175
BsrBI CCGCTC 1 cut(s) 654
BsrFI RCCGGY 2 cut(s) 450, 1107
BsrI ACTGG 3 cut(s) 608, 946, 1207
BssAI RCCGGY 2 cut(s) 450, 1107
BssECI CCNNGG 3 cut(s) 291, 802, 1021
BssMI GATC 6 cut(s) 6, 79, 135, 159, 1078, 1381
BssT1I CCWWGG 1 cut(s) 802
Bst2UI CCWGG 2 cut(s) 292, 1023
Bst4CI ACNGT 1 cut(s) 644
Bst6I CTCTTC 3 cut(s) 1024, 1049, 1163
BstAFI CTTAAG 1 cut(s) 1175
BstAPI GCANNNNNTGC 1 cut(s) 359
BstBAI YACGTR 1 cut(s) 734
BstC8I GCNNGC 2 cut(s) 99, 511
BstDEI CTNAG 4 cut(s) 893, 1060, 1067, 1364
BstF5I GGATG 3 cut(s) 322, 387, 1267
BstFNI CGCG 2 cut(s) 155, 439
BstHHI GCGC 3 cut(s) 155, 441, 509
BstKTI GATC 6 cut(s) 9, 82, 138, 162, 1081, 1384
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 6 cut(s) 6, 79, 135, 159, 1078, 1381
BstMWI GCNNNNNNNGC 5 cut(s) 150, 343, 359, 510, 845
BstNI CCWGG 2 cut(s) 292, 1023
BstSCI CCNGG 2 cut(s) 290, 1021
BstSFI CTRYAG 3 cut(s) 502, 695, 843
BstSNI TACGTA 1 cut(s) 734
BstUI CGCG 2 cut(s) 155, 439
BstV1I GCAGC 7 cut(s) 349, 488, 516, 758, 826, 848, 1139
BstV2I GAAGAC 1 cut(s) 132
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BsuI GTATCC 2 cut(s) 791, 1145
BsuRI GGCC 2 cut(s) 273, 920
BtrI CACGTC 1 cut(s) 1122
BtsCI GGATG 3 cut(s) 322, 387, 1267
BtsIMutI CAGTG 2 cut(s) 89, 890
BveI ACCTGC 2 cut(s) 102, 1205
Cac8I GCNNGC 2 cut(s) 99, 511
CciI TCATGA 1 cut(s) 12
CfoI GCGC 3 cut(s) 155, 441, 509
Cfr10I RCCGGY 2 cut(s) 450, 1107
Csp6I GTAC 5 cut(s) 454, 731, 735, 945, 1141
CspAI ACCGGT 2 cut(s) 450, 1107
CviAII CATG 5 cut(s) 13, 130, 628, 1156, 1330
CviQI GTAC 5 cut(s) 454, 731, 735, 945, 1141
DdeI CTNAG 4 cut(s) 893, 1060, 1067, 1364
DpnI GATC 6 cut(s) 8, 81, 137, 161, 1080, 1383
DpnII GATC 6 cut(s) 6, 79, 135, 159, 1078, 1381
Eam1104I CTCTTC 3 cut(s) 1024, 1049, 1163
EarI CTCTTC 3 cut(s) 1024, 1049, 1163
Eco105I TACGTA 1 cut(s) 734
Eco130I CCWWGG 1 cut(s) 802
Eco57I CTGAAG 1 cut(s) 777
Eco88I CYCGRG 2 cut(s) 490, 655
EcoRII CCWGG 2 cut(s) 290, 1021
EcoT14I CCWWGG 1 cut(s) 802
ErhI CCWWGG 1 cut(s) 802
FaeI CATG 5 cut(s) 16, 133, 631, 1159, 1333
FalI AAGNNNNNCTT 2 cut(s) 149, 181
FaqI GGGAC 1 cut(s) 860
FatI CATG 5 cut(s) 12, 129, 627, 1155, 1329
FauI CCCGC 1 cut(s) 427
Fnu4HI GCNGC 8 cut(s) 363, 502, 505, 613, 772, 837, 840, 1128
FokI GGATG 3 cut(s) 329, 374, 1274
Fsp4HI GCNGC 8 cut(s) 363, 502, 505, 613, 772, 837, 840, 1128
FspBI CTAG 5 cut(s) 782, 872, 924, 962, 1092
GlaI GCGC 3 cut(s) 154, 440, 508
GluI GCNGC 8 cut(s) 363, 502, 505, 613, 772, 837, 840, 1128
HaeIII GGCC 2 cut(s) 273, 920
HapII CCGG 4 cut(s) 228, 451, 1108, 1131
HhaI GCGC 3 cut(s) 155, 441, 509
Hin1II CATG 5 cut(s) 16, 133, 631, 1159, 1333
Hin6I GCGC 3 cut(s) 153, 439, 507
HinP1I GCGC 3 cut(s) 153, 439, 507
HindIII AAGCTT 2 cut(s) 344, 1366
HinfI GANTC 3 cut(s) 146, 178, 587
HpaII CCGG 4 cut(s) 228, 451, 1108, 1131
HphI GGTGA 3 cut(s) 808, 1010, 1097
Hpy166II GTNNAC 1 cut(s) 678
Hpy188I TCNGA 3 cut(s) 71, 933, 1197
Hpy8I GTNNAC 1 cut(s) 678
Hpy99I CGWCG 2 cut(s) 439, 1358
HpyAV CCTTC 6 cut(s) 61, 66, 263, 384, 1174, 1246
HpyCH4III ACNGT 1 cut(s) 644
HpyCH4IV ACGT 3 cut(s) 434, 733, 1121
HpyCH4V TGCA 7 cut(s) 365, 504, 535, 774, 1014, 1227, 1287
HpyF10VI GCNNNNNNNGC 5 cut(s) 150, 343, 359, 510, 845
HpyF3I CTNAG 4 cut(s) 893, 1060, 1067, 1364
HpySE526I ACGT 3 cut(s) 434, 733, 1121
Hsp92II CATG 5 cut(s) 16, 133, 631, 1159, 1333
HspAI GCGC 3 cut(s) 153, 439, 507
Kzo9I GATC 6 cut(s) 6, 79, 135, 159, 1078, 1381
LguI GCTCTTC 2 cut(s) 1024, 1049
LmnI GCTCC 2 cut(s) 601, 1211
Lsp1109I GCAGC 7 cut(s) 349, 488, 516, 758, 826, 848, 1139
LweI GCATC 4 cut(s) 330, 621, 660, 1255
MaeI CTAG 5 cut(s) 782, 872, 924, 962, 1092
MaeII ACGT 3 cut(s) 434, 733, 1121
MaeIII GTNAC 3 cut(s) 89, 717, 1295
MalI GATC 6 cut(s) 8, 81, 137, 161, 1080, 1383
MbiI CCGCTC 1 cut(s) 654
MboI GATC 6 cut(s) 6, 79, 135, 159, 1078, 1381
MflI RGATCY 1 cut(s) 6
MluCI AATT 7 cut(s) 223, 264, 366, 899, 1047, 1191, 1392
MmeI TCCRAC 2 cut(s) 168, 311
MroXI GAANNNNTTC 2 cut(s) 542, 903
MseI TTAA 5 cut(s) 306, 851, 1176, 1221, 1402
MslI CAYNNNNRTG 2 cut(s) 17, 1023
MspA1I CMGCKG 1 cut(s) 839
MspCI CTTAAG 1 cut(s) 1175
MspI CCGG 4 cut(s) 228, 451, 1108, 1131
MspR9I CCNGG 2 cut(s) 292, 1023
Mva1269I GAATGC 3 cut(s) 364, 773, 1014
MvaI CCWGG 2 cut(s) 292, 1023
MvnI CGCG 2 cut(s) 155, 439
MwoI GCNNNNNNNGC 5 cut(s) 150, 343, 359, 510, 845
NdeII GATC 6 cut(s) 6, 79, 135, 159, 1078, 1381
NlaIII CATG 5 cut(s) 16, 133, 631, 1159, 1333
NmeAIII GCCGAG 1 cut(s) 299
OliI CACNNNNGTG 1 cut(s) 1023
PagI TCATGA 1 cut(s) 12
PciSI GCTCTTC 2 cut(s) 1024, 1049
PctI GAATGC 3 cut(s) 364, 773, 1014
PdmI GAANNNNTTC 2 cut(s) 542, 903
PfeI GAWTC 3 cut(s) 146, 178, 587
PflFI GACNNNGTC 2 cut(s) 19, 872
PflMI CCANNNNNTGG 1 cut(s) 1275
PinAI ACCGGT 2 cut(s) 450, 1107
PkrI GCNGC 8 cut(s) 364, 503, 506, 614, 773, 838, 841, 1129
Ppu21I YACGTR 1 cut(s) 734
Psp6I CCWGG 2 cut(s) 290, 1021
PspGI CCWGG 2 cut(s) 290, 1021
PstI CTGCAG 1 cut(s) 506
PsuI RGATCY 1 cut(s) 6
PsyI GACNNNGTC 2 cut(s) 19, 872
PvuII CAGCTG 1 cut(s) 839
RsaI GTAC 5 cut(s) 455, 732, 736, 946, 1142
RsaNI GTAC 5 cut(s) 454, 731, 735, 945, 1141
RseI CAYNNNNRTG 2 cut(s) 17, 1023
SapI GCTCTTC 2 cut(s) 1024, 1049
SaqAI TTAA 5 cut(s) 306, 851, 1176, 1221, 1402
SatI GCNGC 8 cut(s) 363, 502, 505, 613, 772, 837, 840, 1128
Sau3AI GATC 6 cut(s) 6, 79, 135, 159, 1078, 1381
ScrFI CCNGG 2 cut(s) 292, 1023
SfaNI GCATC 4 cut(s) 330, 621, 660, 1255
SfcI CTRYAG 3 cut(s) 502, 695, 843
SmiMI CAYNNNNRTG 2 cut(s) 17, 1023
SmlI CTYRAG 3 cut(s) 989, 1082, 1175
SmoI CTYRAG 3 cut(s) 989, 1082, 1175
SnaBI TACGTA 1 cut(s) 734
Sse9I AATT 7 cut(s) 223, 264, 366, 899, 1047, 1191, 1392
SsiI CCGC 5 cut(s) 169, 420, 569, 612, 652
SspMI CTAG 5 cut(s) 782, 872, 924, 962, 1092
StyD4I CCNGG 2 cut(s) 290, 1021
StyI CCWWGG 1 cut(s) 802
TaaI ACNGT 1 cut(s) 644
TaiI ACGT 3 cut(s) 437, 736, 1124
TaqI TCGA 6 cut(s) 330, 384, 407, 668, 749, 1380
TasI AATT 7 cut(s) 223, 264, 366, 899, 1047, 1191, 1392
TauI GCSGC 1 cut(s) 615
TfiI GAWTC 3 cut(s) 146, 178, 587
Tru1I TTAA 5 cut(s) 306, 851, 1176, 1221, 1402
Tru9I TTAA 5 cut(s) 306, 851, 1176, 1221, 1402
TscAI CASTG 2 cut(s) 89, 897
TseI GCWGC 7 cut(s) 362, 501, 504, 771, 836, 839, 1127
TspDTI ATGAA 3 cut(s) 146, 780, 872
TspRI CASTG 2 cut(s) 89, 897
Tth111I GACNNNGTC 2 cut(s) 19, 872
Van91I CCANNNNNTGG 1 cut(s) 1275
Vha464I CTTAAG 1 cut(s) 1175
XapI RAATTY 2 cut(s) 1191, 1392
XbaI TCTAGA 1 cut(s) 1091
XmnI GAANNNNTTC 2 cut(s) 542, 903
XspI CTAG 5 cut(s) 782, 872, 924, 962, 1092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.