Rroxscaffold_4G00291900

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003355
Physical Location & Seq
Forward (+)
12248073 .. 12249338
1266 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00291900.1

Sequence Viewer

Length: 1266 bp
ATGAAGCTCCAAACCGACACATGTGCGAAAATCGTCATGCAAAACAAGAATCTGGGGCTGGAAGATTTCAGGTTCCTCTTGAAAACCATTGTCAATTCCGGCATTGGTGAGGAAACATCCTGCCCAAGAAACATCCTCCAAAGCCGAGAAGCAACCCTAGCAGATTCACTCTCTGAGATGGATGAAACCATTTTCGATACACTTGACACGCTTTTCGCCAGGTACCCTTCAGTCTCCCCATCACAAATCGACATTCTCGTGGTCAATGTCTCCATGTTCTCCCCTTCACCCTCCCTAACATCTCGAATAGTGAACCGTTACAAGATGAGGGAGGATATCAAGACATTCAACCTCTCCGGAATGGGCTGCAGCGCAAGCCTAGTGGCCATTGATGTTGTCCAGGGCTTGTTCAAGTCATACAAAGACTCATACGCCCTTGTTGTGAGCACAGAGTCCATGGCTCCTTACTGGTATAGCGGCAAGGAAAAATCCATGATGCTTACCAACTGTTTGTTTCGGTTAGGAGGCTGTTCGATGCTGTTCACCAATAGAAGATACCTTAAGCACCAAGCTATCTTGAAATTGAAGACTTTGGTAAGAACACATATCGGCTCGAATGATGATGCTTACAATTCTTGCATACAGCTAGAAGATGATAGTGGTTACCTAGGTTTTCGGCTTACCAAATACCTCACACAAGCAGCAGCTCTAGCTCTCACAATAAACTTAAAAGTTCTAGTGCCAAAAGTACTTCCACTAAGGGAAATACTCCGGTACCTGGTGGAGTCTAGGCTGCTGAAGAAAAGTGGTACCAAGAGCCAAAACCTAGAAGCAGTTGGAGTTGGTTTGAATATGAAGGCCGGAATAGAGCACTTTTGTATCCACCCGGGTGGAAGAGCAATCATAGATGGGATTGGCAAGAGCTTAGCATTAAGCGAGTATGATCTCGAGCCGTCTAGAATGGCGCTTTTCCGATTTGGTAACACATCAGCTGCTGGGTTTTGGTATGCTTTAGGATACATGGAAGCCAAAAAGAGGCTCAAGAAGGGTGATAGGATTCTTATGAGTGGATTTGGAGCAGGTTTTAAGTGCAACAATATTGTTTGGGAAGTACTGAGGGACTTGGATGATGCCAATGTTTGGAAAAGCTGCATAGATAGTTACCCTCCACATATTATAGCCAACCCTTTCATGGAAAAGTTTAGTTGGCTTAATGATGAATGCCTCAACTTTGTTAGGATTGATTTCAGCAAATTGCTCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

421

Amino Acids

47.23

Weight (kDa)

8.85

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 2 - 265 3.2e-101 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 288 - 370 3.3e-12 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 291 - 365 3.6e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1070
Acc65I GGTACC 3 cut(s) 222, 774, 809
AccB1I GGYRCC 3 cut(s) 222, 774, 809
AccB7I CCANNNNNTGG 1 cut(s) 1140
AccIII TCCGGA 1 cut(s) 356
AciI CCGC 1 cut(s) 477
AcoI YGGCCR 1 cut(s) 384
AcuI CTGAAG 2 cut(s) 213, 818
AfaI GTAC 5 cut(s) 224, 750, 776, 811, 1113
AfiI CCNNNNNNNGG 4 cut(s) 778, 1035, 1140, 1192
AflII CTTAAG 1 cut(s) 560
AflIII ACRYGT 1 cut(s) 20
AgsI TTSAA 6 cut(s) 82, 349, 412, 580, 586, 850
AjnI CCWGG 3 cut(s) 218, 399, 777
AleI CACNNNNGTG 1 cut(s) 888
AluBI AGCT 8 cut(s) 7, 572, 646, 707, 713, 924, 992, 1149
AluI AGCT 8 cut(s) 7, 572, 646, 707, 713, 924, 992, 1149
Alw21I GWGCWC 2 cut(s) 449, 873
Alw26I GTCTC 2 cut(s) 238, 274
AlwNI CAGNNNCTG 1 cut(s) 995
Ama87I CYCGRG 2 cut(s) 886, 947
Aor13HI TCCGGA 1 cut(s) 356
AoxI GGCC 2 cut(s) 384, 858
ApeKI GCWGC 7 cut(s) 366, 369, 701, 704, 793, 992, 1149
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
Asp718I GGTACC 3 cut(s) 222, 774, 809
AspA2I CCTAGG 1 cut(s) 667
AspLEI GCGC 2 cut(s) 374, 967
AsuC2I CCSGG 2 cut(s) 887, 888
AsuHPI GGTGA 4 cut(s) 119, 279, 535, 1061
AvaI CYCGRG 2 cut(s) 886, 947
AvrII CCTAGG 1 cut(s) 667
BaeI ACNNNNGTAYC 4 cut(s) 758, 791, 862, 895
BalI TGGCCA 1 cut(s) 386
BanI GGYRCC 3 cut(s) 222, 774, 809
BauI CACGAG 1 cut(s) 257
BbsI GAAGAC 1 cut(s) 593
Bbv12I GWGCWC 2 cut(s) 449, 873
BbvI GCAGC 7 cut(s) 353, 381, 713, 716, 780, 979, 1136
BccI CCATC 3 cut(s) 172, 247, 902
BceAI ACGGC 1 cut(s) 937
BcgI CGANNNNNNTGC 3 cut(s) 5, 39, 1240
BciT130I CCWGG 3 cut(s) 220, 401, 779
BciVI GTATCC 2 cut(s) 890, 1010
BcnI CCSGG 2 cut(s) 887, 888
BcoDI GTCTC 2 cut(s) 238, 274
BfaI CTAG 9 cut(s) 158, 380, 647, 668, 710, 737, 789, 827, 957
BfmI CTRYAG 1 cut(s) 367
BfoI RGCGCY 1 cut(s) 968
BfrI CTTAAG 1 cut(s) 560
BfuAI ACCTGC 1 cut(s) 1070
BfuI GTATCC 2 cut(s) 890, 1010
BisI GCNGC 8 cut(s) 367, 370, 478, 702, 705, 794, 993, 1150
BlnI CCTAGG 1 cut(s) 667
BlpI GCTNAGC 1 cut(s) 925
BlsI GCNGC 8 cut(s) 368, 371, 479, 703, 706, 795, 994, 1151
BmcAI AGTACT 2 cut(s) 750, 1113
Bme1390I CCNGG 5 cut(s) 220, 401, 779, 887, 888
BmeT110I CYCGRG 2 cut(s) 886, 947
BmiI GGNNCC 5 cut(s) 74, 224, 462, 776, 811
BmrFI CCNGG 5 cut(s) 220, 401, 779, 887, 888
BmsI GCATC 4 cut(s) 486, 525, 613, 1120
BpiI GAAGAC 1 cut(s) 593
Bpu1102I GCTNAGC 1 cut(s) 925
BpuEI CTTGAG 1 cut(s) 1025
BpuMI CCSGG 2 cut(s) 887, 888
BsaJI CCNNGG 4 cut(s) 400, 456, 667, 886
BsaWI WCCGGW 2 cut(s) 356, 771
Bsc4I CCNNNNNNNGG 4 cut(s) 778, 1035, 1140, 1192
Bse1I ACTGG 1 cut(s) 473
BseAI TCCGGA 1 cut(s) 356
BseBI CCWGG 3 cut(s) 220, 401, 779
BseDI CCNNGG 4 cut(s) 400, 456, 667, 886
BseGI GGATG 4 cut(s) 116, 132, 187, 1132
BseLI CCNNNNNNNGG 4 cut(s) 778, 1035, 1140, 1192
BseMII CTCAG 2 cut(s) 165, 1106
BseNI ACTGG 1 cut(s) 473
BseXI GCAGC 7 cut(s) 353, 381, 713, 716, 780, 979, 1136
BseYI CCCAGC 1 cut(s) 995
BshFI GGCC 2 cut(s) 386, 860
BshNI GGYRCC 3 cut(s) 222, 774, 809
BsiHKAI GWGCWC 2 cut(s) 449, 873
BsiHKCI CYCGRG 2 cut(s) 886, 947
BsiSI CCGG 5 cut(s) 99, 357, 772, 861, 887
BslFI GGGAC 1 cut(s) 1133
BslI CCNNNNNNNGG 4 cut(s) 778, 1035, 1140, 1192
BsmAI GTCTC 2 cut(s) 238, 274
BsmFI GGGAC 1 cut(s) 1133
BsmI GAATGC 1 cut(s) 1226
BsnI GGCC 2 cut(s) 386, 860
BsoBI CYCGRG 2 cut(s) 886, 947
Bsp1286I GDGCHC 2 cut(s) 449, 873
Bsp13I TCCGGA 1 cut(s) 356
Bsp143I GATC 1 cut(s) 943
Bsp1720I GCTNAGC 1 cut(s) 925
Bsp19I CCATGG 1 cut(s) 456
BspACI CCGC 1 cut(s) 477
BspANI GGCC 2 cut(s) 386, 860
BspCNI CTCAG 2 cut(s) 166, 1107
BspEI TCCGGA 1 cut(s) 356
BspLI GGNNCC 5 cut(s) 74, 224, 462, 776, 811
BspMAI CTGCAG 1 cut(s) 371
BspMI ACCTGC 1 cut(s) 1070
BspQI GCTCTTC 1 cut(s) 889
BspT107I GGYRCC 3 cut(s) 222, 774, 809
BspTI CTTAAG 1 cut(s) 560
BsrI ACTGG 1 cut(s) 473
BssECI CCNNGG 4 cut(s) 400, 456, 667, 886
BssMI GATC 1 cut(s) 943
BssSI CACGAG 1 cut(s) 257
BssT1I CCWWGG 2 cut(s) 456, 667
Bst2BI CACGAG 1 cut(s) 257
Bst2UI CCWGG 3 cut(s) 220, 401, 779
Bst4CI ACNGT 2 cut(s) 317, 509
Bst6I CTCTTC 1 cut(s) 889
BstAFI CTTAAG 1 cut(s) 560
BstC8I GCNNGC 2 cut(s) 376, 1260
BstDEI CTNAG 4 cut(s) 174, 758, 925, 1115
BstDSI CCRYGG 1 cut(s) 456
BstEII GGTNACC 1 cut(s) 662
BstF5I GGATG 4 cut(s) 116, 132, 187, 1132
BstH2I RGCGCY 1 cut(s) 968
BstHHI GCGC 2 cut(s) 374, 967
BstKTI GATC 1 cut(s) 946
BstMAI GTCTC 2 cut(s) 238, 274
BstMBI GATC 1 cut(s) 943
BstMWI GCNNNNNNNGC 3 cut(s) 158, 375, 710
BstNI CCWGG 3 cut(s) 220, 401, 779
BstNSI RCATGY 1 cut(s) 24
BstPI GGTNACC 1 cut(s) 662
BstSCI CCNGG 5 cut(s) 218, 399, 777, 885, 886
BstSFI CTRYAG 1 cut(s) 367
BstV1I GCAGC 7 cut(s) 353, 381, 713, 716, 780, 979, 1136
BstV2I GAAGAC 1 cut(s) 593
BstXI CCANNNNNNTGG 1 cut(s) 890
BsuI GTATCC 2 cut(s) 890, 1010
BsuRI GGCC 2 cut(s) 386, 860
BtgI CCRYGG 1 cut(s) 456
BtsCI GGATG 4 cut(s) 116, 132, 187, 1132
BveI ACCTGC 1 cut(s) 1070
Cac8I GCNNGC 2 cut(s) 376, 1260
CaiI CAGNNNCTG 1 cut(s) 995
CfoI GCGC 2 cut(s) 374, 967
Cfr9I CCCGGG 1 cut(s) 886
CsiI ACCWGGT 1 cut(s) 777
Csp6I GTAC 5 cut(s) 223, 749, 775, 810, 1112
CspCI CAANNNNNGTGG 2 cut(s) 363, 398
CviAII CATG 7 cut(s) 21, 37, 274, 457, 493, 1021, 1192
CviQI GTAC 5 cut(s) 223, 749, 775, 810, 1112
DdeI CTNAG 4 cut(s) 174, 758, 925, 1115
DpnI GATC 1 cut(s) 945
DpnII GATC 1 cut(s) 943
EaeI YGGCCR 1 cut(s) 384
Eam1104I CTCTTC 1 cut(s) 889
EarI CTCTTC 1 cut(s) 889
Eco130I CCWWGG 2 cut(s) 456, 667
Eco32I GATATC 1 cut(s) 337
Eco57I CTGAAG 2 cut(s) 213, 818
Eco88I CYCGRG 2 cut(s) 886, 947
Eco91I GGTNACC 1 cut(s) 662
EcoO65I GGTNACC 1 cut(s) 662
EcoRII CCWGG 3 cut(s) 218, 399, 777
EcoRV GATATC 1 cut(s) 337
EcoT14I CCWWGG 2 cut(s) 456, 667
ErhI CCWWGG 2 cut(s) 456, 667
FaeI CATG 7 cut(s) 24, 40, 277, 460, 496, 1024, 1195
FaqI GGGAC 1 cut(s) 1133
FatI CATG 7 cut(s) 20, 36, 273, 456, 492, 1020, 1191
Fnu4HI GCNGC 8 cut(s) 367, 370, 478, 702, 705, 794, 993, 1150
FokI GGATG 4 cut(s) 103, 119, 194, 1139
Fsp4HI GCNGC 8 cut(s) 367, 370, 478, 702, 705, 794, 993, 1150
FspBI CTAG 9 cut(s) 158, 380, 647, 668, 710, 737, 789, 827, 957
GlaI GCGC 2 cut(s) 373, 966
GluI GCNGC 8 cut(s) 367, 370, 478, 702, 705, 794, 993, 1150
GsaI CCCAGC 1 cut(s) 999
HaeII RGCGCY 1 cut(s) 968
HaeIII GGCC 2 cut(s) 386, 860
HapII CCGG 5 cut(s) 99, 357, 772, 861, 887
HhaI GCGC 2 cut(s) 374, 967
Hin1II CATG 7 cut(s) 24, 40, 277, 460, 496, 1024, 1195
Hin6I GCGC 2 cut(s) 372, 965
HinP1I GCGC 2 cut(s) 372, 965
HinfI GANTC 6 cut(s) 49, 164, 425, 452, 785, 1057
HpaII CCGG 5 cut(s) 99, 357, 772, 861, 887
HphI GGTGA 4 cut(s) 119, 279, 535, 1061
Hpy166II GTNNAC 2 cut(s) 313, 543
Hpy188I TCNGA 2 cut(s) 175, 974
Hpy188III TCNNGA 8 cut(s) 79, 303, 340, 357, 577, 947, 957, 1042
Hpy8I GTNNAC 2 cut(s) 313, 543
HpyAV CCTTC 4 cut(s) 237, 294, 850, 1039
HpyCH4III ACNGT 2 cut(s) 317, 509
HpyCH4V TGCA 5 cut(s) 40, 369, 639, 1092, 1152
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 375, 710
HpyF3I CTNAG 4 cut(s) 174, 758, 925, 1115
Hsp92II CATG 7 cut(s) 24, 40, 277, 460, 496, 1024, 1195
HspAI GCGC 2 cut(s) 372, 965
Kpn2I TCCGGA 1 cut(s) 356
KpnI GGTACC 3 cut(s) 226, 778, 813
Kzo9I GATC 1 cut(s) 943
LguI GCTCTTC 1 cut(s) 889
LmnI GCTCC 3 cut(s) 12, 466, 1076
Lsp1109I GCAGC 7 cut(s) 353, 381, 713, 716, 780, 979, 1136
LweI GCATC 4 cut(s) 486, 525, 613, 1120
MabI ACCWGGT 1 cut(s) 777
MaeI CTAG 9 cut(s) 158, 380, 647, 668, 710, 737, 789, 827, 957
MaeIII GTNAC 4 cut(s) 317, 662, 980, 1160
MalI GATC 1 cut(s) 945
MboI GATC 1 cut(s) 943
MboII GAAGA 6 cut(s) 74, 564, 598, 662, 811, 906
MhlI GDGCHC 2 cut(s) 449, 873
MlsI TGGCCA 1 cut(s) 386
MluCI AATT 4 cut(s) 94, 581, 631, 1253
MluNI TGGCCA 1 cut(s) 386
MlyI GAGTC 3 cut(s) 419, 461, 794
MmeI TCCRAC 1 cut(s) 817
Mox20I TGGCCA 1 cut(s) 386
MroI TCCGGA 1 cut(s) 356
MscI TGGCCA 1 cut(s) 386
MseI TTAA 6 cut(s) 561, 728, 932, 1086, 1212, 1264
MslI CAYNNNNRTG 2 cut(s) 257, 888
Msp20I TGGCCA 1 cut(s) 386
MspA1I CMGCKG 1 cut(s) 992
MspCI CTTAAG 1 cut(s) 560
MspI CCGG 5 cut(s) 99, 357, 772, 861, 887
MspR9I CCNGG 5 cut(s) 220, 401, 779, 887, 888
Mva1269I GAATGC 1 cut(s) 1226
MvaI CCWGG 3 cut(s) 220, 401, 779
MwoI GCNNNNNNNGC 3 cut(s) 158, 375, 710
NciI CCSGG 2 cut(s) 887, 888
NcoI CCATGG 1 cut(s) 456
NdeII GATC 1 cut(s) 943
NlaIII CATG 7 cut(s) 24, 40, 277, 460, 496, 1024, 1195
NlaIV GGNNCC 5 cut(s) 74, 224, 462, 776, 811
NmeAIII GCCGAG 1 cut(s) 170
NspI RCATGY 1 cut(s) 24
OliI CACNNNNGTG 1 cut(s) 888
PaeR7I CTCGAG 1 cut(s) 947
PciI ACATGT 1 cut(s) 20
PciSI GCTCTTC 1 cut(s) 889
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PctI GAATGC 1 cut(s) 1226
PfeI GAWTC 3 cut(s) 49, 164, 1057
PflMI CCANNNNNTGG 1 cut(s) 1140
PkrI GCNGC 8 cut(s) 368, 371, 479, 703, 706, 795, 994, 1151
PleI GAGTC 3 cut(s) 419, 460, 793
PpsI GAGTC 3 cut(s) 419, 460, 793
PscI ACATGT 1 cut(s) 20
Psp6I CCWGG 3 cut(s) 218, 399, 777
PspEI GGTNACC 1 cut(s) 662
PspFI CCCAGC 1 cut(s) 995
PspGI CCWGG 3 cut(s) 218, 399, 777
PspN4I GGNNCC 5 cut(s) 74, 224, 462, 776, 811
PstI CTGCAG 1 cut(s) 371
PstNI CAGNNNCTG 1 cut(s) 995
PvuII CAGCTG 1 cut(s) 992
RsaI GTAC 5 cut(s) 224, 750, 776, 811, 1113
RsaNI GTAC 5 cut(s) 223, 749, 775, 810, 1112
RseI CAYNNNNRTG 2 cut(s) 257, 888
SapI GCTCTTC 1 cut(s) 889
SaqAI TTAA 6 cut(s) 561, 728, 932, 1086, 1212, 1264
SatI GCNGC 8 cut(s) 367, 370, 478, 702, 705, 794, 993, 1150
Sau3AI GATC 1 cut(s) 943
ScaI AGTACT 2 cut(s) 750, 1113
SchI GAGTC 3 cut(s) 419, 461, 794
ScrFI CCNGG 5 cut(s) 220, 401, 779, 887, 888
SduI GDGCHC 2 cut(s) 449, 873
SexAI ACCWGGT 1 cut(s) 777
SfaNI GCATC 4 cut(s) 486, 525, 613, 1120
SfcI CTRYAG 1 cut(s) 367
Sfr274I CTCGAG 1 cut(s) 947
SlaI CTCGAG 1 cut(s) 947
SmaI CCCGGG 1 cut(s) 888
SmiMI CAYNNNNRTG 2 cut(s) 257, 888
SmlI CTYRAG 3 cut(s) 560, 947, 1040
SmoI CTYRAG 3 cut(s) 560, 947, 1040
Sse9I AATT 4 cut(s) 94, 581, 631, 1253
SsiI CCGC 1 cut(s) 477
SspI AATATT 1 cut(s) 1099
SspMI CTAG 9 cut(s) 158, 380, 647, 668, 710, 737, 789, 827, 957
StyD4I CCNGG 5 cut(s) 218, 399, 777, 885, 886
StyI CCWWGG 2 cut(s) 456, 667
TaaI ACNGT 2 cut(s) 317, 509
TaqI TCGA 6 cut(s) 195, 249, 304, 533, 614, 948
TasI AATT 4 cut(s) 94, 581, 631, 1253
TatI WGTACW 2 cut(s) 748, 1111
TauI GCSGC 1 cut(s) 480
TfiI GAWTC 3 cut(s) 49, 164, 1057
Tru1I TTAA 6 cut(s) 561, 728, 932, 1086, 1212, 1264
Tru9I TTAA 6 cut(s) 561, 728, 932, 1086, 1212, 1264
TseI GCWGC 7 cut(s) 366, 369, 701, 704, 793, 992, 1149
TspDTI ATGAA 5 cut(s) 17, 198, 869, 1180, 1233
TspMI CCCGGG 1 cut(s) 886
Van91I CCANNNNNTGG 1 cut(s) 1140
Vha464I CTTAAG 1 cut(s) 560
XbaI TCTAGA 1 cut(s) 956
XceI RCATGY 1 cut(s) 24
XcmI CCANNNNNNNNNTGG 1 cut(s) 1189
XhoI CTCGAG 1 cut(s) 947
XmaI CCCGGG 1 cut(s) 886
XmaJI CCTAGG 1 cut(s) 667
XspI CTAG 9 cut(s) 158, 380, 647, 668, 710, 737, 789, 827, 957
ZrmI AGTACT 2 cut(s) 750, 1113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.