AT5G14450

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002827
Physical Location & Seq
Forward (+)
4658441 .. 4660175
1735 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G14450.1

Sequence Viewer

Length: 1170 bp
ATGAAGGATAATCTTGAAAGAGCAAAACTTATGGTTTCTTCTACTGTGTTTTCATGGCTTCTTCTGTGTCTCTTTGCAGTGACAACATCTGTTTCAGTTCAGCCTACGTGTACTTTTCCGGCGATTTACAACTTCGGAGATTCTAATTCCGACACTGGAGGTATCTCCGCCGCTTTTGAGCCTATTAGGGATCCTTACGGTCAAGGTTTCTTCCACAGACCCACCGGAAGAGACTCCGATGGCCGTCTCACCATTGATTTCATTGCTGAGCGTCTGGGGTTACCGTATTTGAGCGCATACTTAAACTCGTTAGGATCAAACTTCAGACATGGAGCAAACTTTGCAACAGGTGGTTCAACCATAAGAAGACAGAACGAAACTATCTTTCAATATGGCATTAGTCCATTCTCTCTCGATATGCAGATTGCTCAATTCGATCAGTTCAAAGCCAGATCTGCACTACTCTTCACACAAATTAAGAGTAGATATGATAGAGAGAAGCTTCCAAGACAGGAAGAGTTTGCAAAGGCTTTGTATACTTTTGATATTGGACAGAATGATCTCTCTGTTGGGTTTCGGACAATGAGCGTCGATCAACTTAAGGCGACGATACCCGACATTGTCAACCATTTAGCTTCTGCTGTCCGGAACATATATCAGCAAGGAGGAAGAACATTTTGGGTACACAACACGGGTCCTTTCGGTTGTTTACCCGTGAACATGTTTTACATGGGCACTCCTGCACCGGGCTACTTGGACAAATCTGGCTGCGTGAAGGCTCAAAATGAAATGGCGATGGAGTTCAACAGAAAGCTCAAGGAAACAGTAATCAATCTCAGAAAAGAACTCACTCAAGCAGCCATTACATATGTTGATGTCTACACTGCGAAATATGAAATGATGAGCAATCCCAAGAAACTAGGATTTGCAAACCCCTTGAAGGTATGTTGCGGCTACCATGAGAAGTATGATCACATCTGGTGTGGAAACAAAGGGAAAGTGAACAATACCGAAATCTACGGTGGCTCTTGTCCCAACCCGGTAATGGCTGTGAGCTGGGATGGAGTTCACTATACGGAAGCTGCAAACAAGCATGTGGCTGACCGTACTCTAAATGGTTTGCTCACAGACCCTCCAGTTCCAATAACCCGAGCTTGTTATAGACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

389

Amino Acids

43.57

Weight (kDa)

8.72

Isoelectric Point (pI)

34.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 42 - 369 4.1e-48 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 536, 879
AccIII TCCGGA 1 cut(s) 645
AciI CCGC 3 cut(s) 168, 171, 951
AclWI GGATC 3 cut(s) 185, 198, 322
AcoI YGGCCR 1 cut(s) 241
AcuI CTGAAG 1 cut(s) 307
AfaI GTAC 3 cut(s) 112, 684, 1108
AfiI CCNNNNNNNGG 3 cut(s) 746, 940, 1045
AflII CTTAAG 1 cut(s) 599
AflIII ACRYGT 2 cut(s) 107, 720
AgsI TTSAA 6 cut(s) 17, 357, 389, 445, 805, 940
AjuI GAANNNNNNNTTGG 2 cut(s) 661, 693
AluBI AGCT 6 cut(s) 502, 635, 814, 1056, 1082, 1154
AluI AGCT 6 cut(s) 502, 635, 814, 1056, 1082, 1154
Alw26I GTCTC 3 cut(s) 74, 225, 251
AlwI GGATC 3 cut(s) 185, 198, 322
Ama87I CYCGRG 1 cut(s) 1149
Aor13HI TCCGGA 1 cut(s) 645
AoxI GGCC 1 cut(s) 241
ApeKI GCWGC 3 cut(s) 768, 857, 1082
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 695
AsuC2I CCSGG 2 cut(s) 747, 1040
AsuHPI GGTGA 1 cut(s) 241
AvaI CYCGRG 1 cut(s) 1149
AvaII GGWCC 1 cut(s) 695
BaeGI GKGCMC 1 cut(s) 737
BamHI GGATCC 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 373
BbvI GCAGC 3 cut(s) 755, 869, 1069
BccI CCATC 3 cut(s) 233, 790, 1055
BceAI ACGGC 1 cut(s) 228
BclI TGATCA 1 cut(s) 970
BcnI CCSGG 2 cut(s) 747, 1040
BcoDI GTCTC 3 cut(s) 74, 225, 251
BfaI CTAG 1 cut(s) 920
BfrI CTTAAG 1 cut(s) 599
BglII AGATCT 1 cut(s) 452
BisI GCNGC 5 cut(s) 171, 769, 858, 952, 1083
BlpI GCTNAGC 1 cut(s) 267
BlsI GCNGC 5 cut(s) 172, 770, 859, 953, 1084
Bme1390I CCNGG 2 cut(s) 747, 1040
Bme18I GGWCC 1 cut(s) 695
BmeT110I CYCGRG 1 cut(s) 1149
BmgT120I GGNCC 1 cut(s) 695
BmiI GGNNCC 2 cut(s) 192, 696
BmrFI CCNGG 2 cut(s) 747, 1040
BpiI GAAGAC 1 cut(s) 373
BpmI CTGGAG 2 cut(s) 177, 1119
Bpu1102I GCTNAGC 1 cut(s) 267
BpuEI CTTGAG 2 cut(s) 800, 837
BpuMI CCSGG 2 cut(s) 747, 1040
BsaAI YACGTR 1 cut(s) 108
BsaWI WCCGGW 2 cut(s) 224, 645
BsaXI ACNNNNNCTCC 2 cut(s) 1117, 1147
Bsc4I CCNNNNNNNGG 3 cut(s) 746, 940, 1045
Bse1I ACTGG 2 cut(s) 160, 1136
Bse3DI GCAATG 1 cut(s) 261
BseAI TCCGGA 1 cut(s) 645
BseGI GGATG 1 cut(s) 1066
BseLI CCNNNNNNNGG 3 cut(s) 746, 940, 1045
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 258, 850
BseNI ACTGG 2 cut(s) 160, 1136
BseSI GKGCMC 1 cut(s) 737
BseXI GCAGC 3 cut(s) 755, 869, 1069
BseYI CCCAGC 1 cut(s) 1056
BsgI GTGCAG 2 cut(s) 441, 726
BshFI GGCC 1 cut(s) 243
BsiHKCI CYCGRG 1 cut(s) 1149
BsiSI CCGG 5 cut(s) 119, 225, 646, 746, 1040
BslFI GGGAC 1 cut(s) 1017
BslI CCNNNNNNNGG 3 cut(s) 746, 940, 1045
BsmAI GTCTC 3 cut(s) 74, 225, 251
BsmBI CGTCTC 1 cut(s) 251
BsmFI GGGAC 1 cut(s) 1017
BsnI GGCC 1 cut(s) 243
BsoBI CYCGRG 1 cut(s) 1149
Bsp1286I GDGCHC 1 cut(s) 737
Bsp13I TCCGGA 1 cut(s) 645
Bsp143I GATC 7 cut(s) 190, 314, 436, 452, 559, 592, 970
Bsp1720I GCTNAGC 1 cut(s) 267
BspACI CCGC 3 cut(s) 168, 171, 951
BspANI GGCC 1 cut(s) 243
BspCNI CTCAG 2 cut(s) 259, 849
BspEI TCCGGA 1 cut(s) 645
BspLI GGNNCC 2 cut(s) 192, 696
BspPI GGATC 3 cut(s) 185, 198, 322
BspTI CTTAAG 1 cut(s) 599
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 2 cut(s) 160, 1136
BssMI GATC 7 cut(s) 190, 314, 436, 452, 559, 592, 970
BssNAI GTATAC 1 cut(s) 537
Bst1107I GTATAC 1 cut(s) 537
Bst4CI ACNGT 7 cut(s) 46, 200, 285, 826, 1022, 1106, 1167
Bst6I CTCTTC 3 cut(s) 223, 470, 510
BstAFI CTTAAG 1 cut(s) 599
BstAPI GCANNNNNTGC 1 cut(s) 341
BstBAI YACGTR 1 cut(s) 108
BstDEI CTNAG 2 cut(s) 267, 836
BstEII GGTNACC 1 cut(s) 279
BstF5I GGATG 1 cut(s) 1066
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 7 cut(s) 193, 317, 439, 455, 562, 595, 973
BstMAI GTCTC 3 cut(s) 74, 225, 251
BstMBI GATC 7 cut(s) 190, 314, 436, 452, 559, 592, 970
BstMWI GCNNNNNNNGC 2 cut(s) 341, 455
BstNSI RCATGY 2 cut(s) 724, 1097
BstPI GGTNACC 1 cut(s) 279
BstSCI CCNGG 2 cut(s) 745, 1038
BstSLI GKGCMC 1 cut(s) 737
BstV1I GCAGC 3 cut(s) 755, 869, 1069
BstV2I GAAGAC 1 cut(s) 373
BstX2I RGATCY 2 cut(s) 190, 452
BstYI RGATCY 2 cut(s) 190, 452
BstZ17I GTATAC 1 cut(s) 537
BsuRI GGCC 1 cut(s) 243
BtgZI GCGATG 1 cut(s) 809
BtsCI GGATG 1 cut(s) 1066
BtsI GCAGTG 2 cut(s) 84, 882
BtsIMutI CAGTG 3 cut(s) 84, 153, 882
CfoI GCGC 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 695
CseI GACGC 2 cut(s) 260, 577
Csp6I GTAC 3 cut(s) 111, 683, 1107
CviAII CATG 6 cut(s) 54, 329, 721, 730, 959, 1094
CviQI GTAC 3 cut(s) 111, 683, 1107
DdeI CTNAG 2 cut(s) 267, 836
DpnI GATC 7 cut(s) 192, 316, 438, 454, 561, 594, 972
DpnII GATC 7 cut(s) 190, 314, 436, 452, 559, 592, 970
EaeI YGGCCR 1 cut(s) 241
Eam1104I CTCTTC 3 cut(s) 223, 470, 510
EarI CTCTTC 3 cut(s) 223, 470, 510
EciI GGCGGA 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 695
Eco57I CTGAAG 1 cut(s) 307
Eco88I CYCGRG 1 cut(s) 1149
Eco91I GGTNACC 1 cut(s) 279
EcoO109I RGGNCCY 1 cut(s) 695
EcoO65I GGTNACC 1 cut(s) 279
Esp3I CGTCTC 1 cut(s) 251
FaeI CATG 6 cut(s) 57, 332, 724, 733, 962, 1097
FaqI GGGAC 1 cut(s) 1017
FatI CATG 6 cut(s) 53, 328, 720, 729, 958, 1093
FauNDI CATATG 1 cut(s) 868
FbaI TGATCA 1 cut(s) 970
FblI GTMKAC 2 cut(s) 536, 879
Fnu4HI GCNGC 5 cut(s) 171, 769, 858, 952, 1083
FokI GGATG 1 cut(s) 1073
Fsp4HI GCNGC 5 cut(s) 171, 769, 858, 952, 1083
FspBI CTAG 1 cut(s) 920
GlaI GCGC 1 cut(s) 295
GluI GCNGC 5 cut(s) 171, 769, 858, 952, 1083
GsaI CCCAGC 1 cut(s) 1060
GsuI CTGGAG 2 cut(s) 177, 1119
HaeIII GGCC 1 cut(s) 243
HapII CCGG 5 cut(s) 119, 225, 646, 746, 1040
HgaI GACGC 2 cut(s) 260, 577
HhaI GCGC 1 cut(s) 296
Hin1II CATG 6 cut(s) 57, 332, 724, 733, 962, 1097
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HindIII AAGCTT 1 cut(s) 500
HinfI GANTC 2 cut(s) 140, 233
HpaII CCGG 5 cut(s) 119, 225, 646, 746, 1040
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 9 cut(s) 111, 537, 625, 685, 710, 718, 880, 1003, 1069
Hpy188I TCNGA 6 cut(s) 137, 151, 238, 326, 579, 839
Hpy188III TCNNGA 3 cut(s) 14, 413, 646
Hpy8I GTNNAC 9 cut(s) 111, 537, 625, 685, 710, 718, 880, 1003, 1069
Hpy99I CGWCG 2 cut(s) 593, 610
HpyAV CCTTC 2 cut(s) 769, 934
HpyCH4III ACNGT 7 cut(s) 46, 200, 285, 826, 1022, 1106, 1167
HpyCH4IV ACGT 1 cut(s) 107
HpyCH4V TGCA 8 cut(s) 77, 344, 421, 458, 524, 743, 929, 1085
HpyF10VI GCNNNNNNNGC 2 cut(s) 341, 455
HpyF3I CTNAG 2 cut(s) 267, 836
HpySE526I ACGT 1 cut(s) 107
Hsp92II CATG 6 cut(s) 57, 332, 724, 733, 962, 1097
HspAI GCGC 1 cut(s) 294
Kpn2I TCCGGA 1 cut(s) 645
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 7 cut(s) 190, 314, 436, 452, 559, 592, 970
LmnI GCTCC 1 cut(s) 332
Lsp1109I GCAGC 3 cut(s) 755, 869, 1069
MaeI CTAG 1 cut(s) 920
MaeII ACGT 1 cut(s) 107
MaeIII GTNAC 2 cut(s) 79, 279
MalI GATC 7 cut(s) 192, 316, 438, 454, 561, 594, 972
MboI GATC 7 cut(s) 190, 314, 436, 452, 559, 592, 970
MboII GAAGA 8 cut(s) 30, 53, 202, 240, 378, 457, 527, 681
MflI RGATCY 2 cut(s) 190, 452
MhlI GDGCHC 1 cut(s) 737
MluCI AATT 3 cut(s) 145, 431, 474
MlyI GAGTC 1 cut(s) 227
MmeI TCCRAC 1 cut(s) 174
MnlI CCTC 3 cut(s) 152, 659, 1143
MroI TCCGGA 1 cut(s) 645
MseI TTAA 3 cut(s) 302, 477, 600
MspCI CTTAAG 1 cut(s) 599
MspI CCGG 5 cut(s) 119, 225, 646, 746, 1040
MspR9I CCNGG 2 cut(s) 747, 1040
MwoI GCNNNNNNNGC 2 cut(s) 341, 455
NciI CCSGG 2 cut(s) 747, 1040
NdeI CATATG 1 cut(s) 868
NdeII GATC 7 cut(s) 190, 314, 436, 452, 559, 592, 970
NlaIII CATG 6 cut(s) 57, 332, 724, 733, 962, 1097
NlaIV GGNNCC 2 cut(s) 192, 696
NmuCI GTSAC 1 cut(s) 79
NspI RCATGY 2 cut(s) 724, 1097
PciI ACATGT 1 cut(s) 720
PfeI GAWTC 1 cut(s) 140
PflFI GACNNNGTC 1 cut(s) 620
PkrI GCNGC 5 cut(s) 172, 770, 859, 953, 1084
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
Ppu21I YACGTR 1 cut(s) 108
PpuMI RGGWCCY 1 cut(s) 695
PscI ACATGT 1 cut(s) 720
Psp5II RGGWCCY 1 cut(s) 695
PspEI GGTNACC 1 cut(s) 279
PspFI CCCAGC 1 cut(s) 1056
PspN4I GGNNCC 2 cut(s) 192, 696
PspPI GGNCC 1 cut(s) 695
PspPPI RGGWCCY 1 cut(s) 695
PsrI GAACNNNNNNTAC 2 cut(s) 710, 742
PsuI RGATCY 2 cut(s) 190, 452
PsyI GACNNNGTC 1 cut(s) 620
RsaI GTAC 3 cut(s) 112, 684, 1108
RsaNI GTAC 3 cut(s) 111, 683, 1107
SaqAI TTAA 3 cut(s) 302, 477, 600
SatI GCNGC 5 cut(s) 171, 769, 858, 952, 1083
Sau3AI GATC 7 cut(s) 190, 314, 436, 452, 559, 592, 970
Sau96I GGNCC 1 cut(s) 695
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 2 cut(s) 747, 1040
SduI GDGCHC 1 cut(s) 737
SinI GGWCC 1 cut(s) 695
SmlI CTYRAG 3 cut(s) 599, 815, 852
SmoI CTYRAG 3 cut(s) 599, 815, 852
Sse9I AATT 3 cut(s) 145, 431, 474
SsiI CCGC 3 cut(s) 168, 171, 951
SspMI CTAG 1 cut(s) 920
StyD4I CCNGG 2 cut(s) 745, 1038
TaaI ACNGT 7 cut(s) 46, 200, 285, 826, 1022, 1106, 1167
TaiI ACGT 1 cut(s) 110
TaqI TCGA 3 cut(s) 414, 435, 591
TasI AATT 3 cut(s) 145, 431, 474
TatI WGTACW 1 cut(s) 110
TauI GCSGC 2 cut(s) 173, 954
TfiI GAWTC 1 cut(s) 140
Tru1I TTAA 3 cut(s) 302, 477, 600
Tru9I TTAA 3 cut(s) 302, 477, 600
TscAI CASTG 3 cut(s) 84, 160, 889
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 3 cut(s) 768, 857, 1082
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 5 cut(s) 17, 42, 250, 801, 909
TspGWI ACGGA 1 cut(s) 1091
TspRI CASTG 3 cut(s) 84, 160, 889
Tth111I GACNNNGTC 1 cut(s) 620
Vha464I CTTAAG 1 cut(s) 599
VpaK11BI GGWCC 1 cut(s) 695
XceI RCATGY 2 cut(s) 724, 1097
XcmI CCANNNNNNNNNTGG 1 cut(s) 1042
XmiI GTMKAC 2 cut(s) 536, 879
XspI CTAG 1 cut(s) 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.