MD09G1021900.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002827
Physical Location & Seq
Forward (+)
1332414 .. 1333631
1218 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1021900.v1.1.491

Sequence Viewer

Length: 807 bp
ATGGAGATGGCTAGATTTCTCGCTGTCGGTTTTTTCGCAATGTGTTTACTGAGCGTGGGAGTGGAAGGGGTGACATACACATCGACTAGTTGTGATTTTCCGGCAATTTACAACTTTGGTGACTCAAATTCAGACACTGGTGCTAACTCGGCAGCATTTTACCCCATGGCATCACCTTGTGGTGAGACTTTCTTCCACAGACCCGCCGGAAGGGGTTGTGACGGCCGTCTCATCATAGATTTTATTGCAAAGCATTTGGGGTTGCCGTACATGAGTCCATATTTGGACTCACTAGAGAGCAATTTCAGGCGCGGTGCCAATTTTGCTAGCGGCGGAGCAACCATCCGGCGTTACAATGAGTCCATGTCTGAAAATGGTGTGAGCCCTTTTTATCTTGACATCCAGATTGCTCAGTATACCCAGTTCAAGTCACGTACCACTCGTCTTTCCAGCCAAGCCGAGGAACAATCCGACAGATTCCCAATAGTCGATGACTTCACAAAGGCGCTCTACACTTTCGATATAGGACAAAATGATCTAGCTGCAGGTTTTCGGAAAAAATTTAGCAGTGAACAGTTTGAAGCACAAATCACAGACATGATTAACCAGCAAGCTAACGCCGTACGAAATTTGTATGAACAAGGGGCAAGGACATTCTGGATACACAACACGGGTCCGATTGGTTGCTTGGCAGTGACGTTACACAACATCCATAATCCGAGCCCCGGCTTGGTTGACGGCCAGGGTTGCGACAAGTTTCAAAATGACATGCCAGACAGTTCAATAGGCAGCTGGAGCAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

269

Amino Acids

29.67

Weight (kDa)

5.36

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 256 2.5e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 536
AccB1I GGYRCC 1 cut(s) 314
AccI GTMKAC 1 cut(s) 416
AccII CGCG 1 cut(s) 312
AciI CCGC 4 cut(s) 204, 312, 330, 333
AcoI YGGCCR 2 cut(s) 223, 739
AcsI RAATTY 3 cut(s) 127, 560, 628
AdeI CACNNNGTG 1 cut(s) 179
AfaI GTAC 3 cut(s) 269, 436, 624
AfiI CCNNNNNNNGG 4 cut(s) 210, 460, 725, 730
AgsI TTSAA 4 cut(s) 427, 581, 761, 783
AhlI ACTAGT 1 cut(s) 86
AjnI CCWGG 1 cut(s) 741
AluBI AGCT 3 cut(s) 542, 614, 792
AluI AGCT 3 cut(s) 542, 614, 792
Alw26I GTCTC 2 cut(s) 179, 233
AlwNI CAGNNNCTG 1 cut(s) 137
AoxI GGCC 2 cut(s) 223, 739
ApeKI GCWGC 3 cut(s) 152, 542, 789
ApoI RAATTY 3 cut(s) 127, 560, 628
AspLEI GCGC 2 cut(s) 312, 508
AspS9I GGNCC 1 cut(s) 674
AsuC2I CCSGG 1 cut(s) 726
AsuHPI GGTGA 4 cut(s) 82, 131, 165, 194
AsuNHI GCTAGC 1 cut(s) 326
AvaII GGWCC 1 cut(s) 674
BanI GGYRCC 1 cut(s) 314
BanII GRGCYC 2 cut(s) 386, 725
BbvI GCAGC 3 cut(s) 164, 529, 801
BccI CCATC 1 cut(s) 350
BceAI ACGGC 5 cut(s) 210, 238, 250, 605, 754
BciT130I CCWGG 1 cut(s) 743
BciVI GTATCC 1 cut(s) 654
BcnI CCSGG 1 cut(s) 726
BcoDI GTCTC 2 cut(s) 179, 233
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 5 cut(s) 12, 87, 293, 327, 539
BfmI CTRYAG 1 cut(s) 543
BfoI RGCGCY 1 cut(s) 509
BfuAI ACCTGC 1 cut(s) 536
BfuI GTATCC 1 cut(s) 654
BisI GCNGC 4 cut(s) 153, 331, 543, 790
BlsI GCNGC 4 cut(s) 154, 332, 544, 791
Bme1390I CCNGG 2 cut(s) 726, 743
Bme18I GGWCC 1 cut(s) 674
BmgT120I GGNCC 1 cut(s) 674
BmiI GGNNCC 2 cut(s) 316, 675
BmrFI CCNGG 2 cut(s) 726, 743
BmrI ACTGGG 1 cut(s) 415
BmsI GCATC 1 cut(s) 179
BmtI GCTAGC 1 cut(s) 330
BmuI ACTGGG 1 cut(s) 415
BoxI GACNNNNGTC 1 cut(s) 225
BpuMI CCSGG 1 cut(s) 726
BsaAI YACGTR 1 cut(s) 434
BsaJI CCNNGG 4 cut(s) 165, 459, 724, 742
Bsc4I CCNNNNNNNGG 4 cut(s) 210, 460, 725, 730
Bse1I ACTGG 2 cut(s) 142, 421
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 1 cut(s) 743
BseDI CCNNGG 4 cut(s) 165, 459, 724, 742
BseGI GGATG 3 cut(s) 342, 399, 708
BseLI CCNNNNNNNGG 4 cut(s) 210, 460, 725, 730
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 2 cut(s) 41, 425
BseNI ACTGG 2 cut(s) 142, 421
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 3 cut(s) 164, 529, 801
Bsh1236I CGCG 1 cut(s) 312
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 2 cut(s) 225, 741
BshNI GGYRCC 1 cut(s) 314
BsiEI CGRYCG 1 cut(s) 226
BsiSI CCGG 4 cut(s) 101, 207, 346, 726
BsiWI CGTACG 1 cut(s) 622
BslI CCNNNNNNNGG 4 cut(s) 210, 460, 725, 730
BsmAI GTCTC 2 cut(s) 179, 233
BsmBI CGTCTC 1 cut(s) 233
BsnI GGCC 2 cut(s) 225, 741
Bsp1286I GDGCHC 2 cut(s) 386, 725
Bsp143I GATC 1 cut(s) 535
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 4 cut(s) 204, 312, 330, 333
BspANI GGCC 2 cut(s) 225, 741
BspCNI CTCAG 2 cut(s) 42, 424
BspFNI CGCG 1 cut(s) 312
BspLI GGNNCC 2 cut(s) 316, 675
BspMAI CTGCAG 1 cut(s) 547
BspMI ACCTGC 1 cut(s) 536
BspOI GCTAGC 1 cut(s) 330
BspT107I GGYRCC 1 cut(s) 314
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 2 cut(s) 142, 421
BssECI CCNNGG 4 cut(s) 165, 459, 724, 742
BssMI GATC 1 cut(s) 535
BssNAI GTATAC 1 cut(s) 417
BssT1I CCWWGG 1 cut(s) 165
Bst1107I GTATAC 1 cut(s) 417
Bst2UI CCWGG 1 cut(s) 743
Bst4CI ACNGT 2 cut(s) 576, 779
BstBAI YACGTR 1 cut(s) 434
BstC8I GCNNGC 2 cut(s) 328, 612
BstDEI CTNAG 2 cut(s) 50, 411
BstDSI CCRYGG 1 cut(s) 165
BstF5I GGATG 3 cut(s) 342, 399, 708
BstFNI CGCG 1 cut(s) 312
BstH2I RGCGCY 1 cut(s) 509
BstHHI GCGC 2 cut(s) 312, 508
BstKTI GATC 1 cut(s) 538
BstMAI GTCTC 2 cut(s) 179, 233
BstMBI GATC 1 cut(s) 535
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 4 cut(s) 149, 323, 747, 795
BstNI CCWGG 1 cut(s) 743
BstNSI RCATGY 1 cut(s) 772
BstPAI GACNNNNGTC 1 cut(s) 225
BstSCI CCNGG 2 cut(s) 724, 741
BstSFI CTRYAG 1 cut(s) 543
BstUI CGCG 1 cut(s) 312
BstV1I GCAGC 3 cut(s) 164, 529, 801
BstZ17I GTATAC 1 cut(s) 417
BstZI CGGCCG 1 cut(s) 223
BsuI GTATCC 1 cut(s) 654
BsuRI GGCC 2 cut(s) 225, 741
BtgI CCRYGG 1 cut(s) 165
BtsCI GGATG 3 cut(s) 342, 399, 708
BtsI GCAGTG 2 cut(s) 574, 699
BtsIMutI CAGTG 3 cut(s) 135, 574, 699
BveI ACCTGC 1 cut(s) 536
Cac8I GCNNGC 2 cut(s) 328, 612
CaiI CAGNNNCTG 1 cut(s) 137
CfoI GCGC 2 cut(s) 312, 508
Cfr13I GGNCC 1 cut(s) 674
Csp6I GTAC 3 cut(s) 268, 435, 623
CviAII CATG 5 cut(s) 166, 271, 364, 598, 769
CviQI GTAC 3 cut(s) 268, 435, 623
DdeI CTNAG 2 cut(s) 50, 411
DpnI GATC 1 cut(s) 537
DpnII GATC 1 cut(s) 535
DraIII CACNNNGTG 1 cut(s) 179
EaeI YGGCCR 2 cut(s) 223, 739
EagI CGGCCG 1 cut(s) 223
EciI GGCGGA 1 cut(s) 348
EclXI CGGCCG 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 2 cut(s) 386, 725
Eco47I GGWCC 1 cut(s) 674
Eco52I CGGCCG 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 741
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 2 cut(s) 386, 725
ErhI CCWWGG 1 cut(s) 165
Esp3I CGTCTC 1 cut(s) 233
FaeI CATG 5 cut(s) 169, 274, 367, 601, 772
FatI CATG 5 cut(s) 165, 270, 363, 597, 768
FauI CCCGC 1 cut(s) 211
FblI GTMKAC 1 cut(s) 416
Fnu4HI GCNGC 4 cut(s) 153, 331, 543, 790
FokI GGATG 3 cut(s) 329, 386, 695
FriOI GRGCYC 2 cut(s) 386, 725
Fsp4HI GCNGC 4 cut(s) 153, 331, 543, 790
FspBI CTAG 5 cut(s) 12, 87, 293, 327, 539
GlaI GCGC 2 cut(s) 311, 507
GluI GCNGC 4 cut(s) 153, 331, 543, 790
HaeII RGCGCY 1 cut(s) 509
HaeIII GGCC 2 cut(s) 225, 741
HapII CCGG 4 cut(s) 101, 207, 346, 726
HhaI GCGC 2 cut(s) 312, 508
Hin1II CATG 5 cut(s) 169, 274, 367, 601, 772
Hin6I GCGC 2 cut(s) 310, 506
HinP1I GCGC 2 cut(s) 310, 506
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HinfI GANTC 5 cut(s) 122, 274, 287, 359, 477
HpaII CCGG 4 cut(s) 101, 207, 346, 726
HphI GGTGA 4 cut(s) 82, 131, 165, 194
Hpy166II GTNNAC 4 cut(s) 47, 417, 572, 736
Hpy188I TCNGA 6 cut(s) 133, 370, 472, 555, 678, 720
Hpy188III TCNNGA 3 cut(s) 395, 403, 658
Hpy8I GTNNAC 4 cut(s) 47, 417, 572, 736
HpyAV CCTTC 2 cut(s) 59, 204
HpyCH4III ACNGT 2 cut(s) 576, 779
HpyCH4IV ACGT 2 cut(s) 433, 698
HpyCH4V TGCA 2 cut(s) 248, 545
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 323, 747, 795
HpyF3I CTNAG 2 cut(s) 50, 411
HpySE526I ACGT 2 cut(s) 433, 698
Hsp92II CATG 5 cut(s) 169, 274, 367, 601, 772
HspAI GCGC 2 cut(s) 310, 506
Kzo9I GATC 1 cut(s) 535
LmnI GCTCC 2 cut(s) 335, 795
Lsp1109I GCAGC 3 cut(s) 164, 529, 801
LweI GCATC 1 cut(s) 179
MaeI CTAG 5 cut(s) 12, 87, 293, 327, 539
MaeII ACGT 2 cut(s) 433, 698
MaeIII GTNAC 7 cut(s) 70, 119, 218, 350, 429, 694, 699
MalI GATC 1 cut(s) 537
MboI GATC 1 cut(s) 535
MboII GAAGA 1 cut(s) 184
MhlI GDGCHC 2 cut(s) 386, 725
MluCI AATT 6 cut(s) 105, 127, 301, 319, 560, 628
MlyI GAGTC 4 cut(s) 116, 281, 283, 368
MmeI TCCRAC 1 cut(s) 495
MnlI CCTC 1 cut(s) 454
MseI TTAA 1 cut(s) 603
MslI CAYNNNNRTG 1 cut(s) 596
MspA1I CMGCKG 1 cut(s) 792
MspI CCGG 4 cut(s) 101, 207, 346, 726
MspR9I CCNGG 2 cut(s) 726, 743
MvaI CCWGG 1 cut(s) 743
MvnI CGCG 1 cut(s) 312
MwoI GCNNNNNNNGC 4 cut(s) 149, 323, 747, 795
NciI CCSGG 1 cut(s) 726
NcoI CCATGG 1 cut(s) 165
NdeII GATC 1 cut(s) 535
NheI GCTAGC 1 cut(s) 326
NlaIII CATG 5 cut(s) 169, 274, 367, 601, 772
NlaIV GGNNCC 2 cut(s) 316, 675
NmeAIII GCCGAG 2 cut(s) 128, 484
NmuCI GTSAC 5 cut(s) 70, 119, 218, 429, 694
NspI RCATGY 1 cut(s) 772
PcsI WCGNNNNNNNCGW 1 cut(s) 439
PfeI GAWTC 1 cut(s) 477
Pfl23II CGTACG 1 cut(s) 622
PkrI GCNGC 4 cut(s) 154, 332, 544, 791
PleI GAGTC 4 cut(s) 116, 281, 282, 367
PpsI GAGTC 4 cut(s) 116, 281, 282, 367
Ppu21I YACGTR 1 cut(s) 434
PshAI GACNNNNGTC 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 741
PspGI CCWGG 1 cut(s) 741
PspLI CGTACG 1 cut(s) 622
PspN4I GGNNCC 2 cut(s) 316, 675
PspPI GGNCC 1 cut(s) 674
PsrI GAACNNNNNNTAC 2 cut(s) 407, 439
PstI CTGCAG 1 cut(s) 547
PstNI CAGNNNCTG 1 cut(s) 137
PvuII CAGCTG 1 cut(s) 792
RsaI GTAC 3 cut(s) 269, 436, 624
RsaNI GTAC 3 cut(s) 268, 435, 623
RseI CAYNNNNRTG 1 cut(s) 596
SaqAI TTAA 1 cut(s) 603
SatI GCNGC 4 cut(s) 153, 331, 543, 790
Sau3AI GATC 1 cut(s) 535
Sau96I GGNCC 1 cut(s) 674
SchI GAGTC 4 cut(s) 116, 281, 283, 368
ScrFI CCNGG 2 cut(s) 726, 743
SduI GDGCHC 2 cut(s) 386, 725
SetI ASST 7 cut(s) 178, 436, 544, 550, 616, 701, 794
SfaNI GCATC 1 cut(s) 179
SfcI CTRYAG 1 cut(s) 543
SinI GGWCC 1 cut(s) 674
SmiMI CAYNNNNRTG 1 cut(s) 596
SpeI ACTAGT 1 cut(s) 86
Sse9I AATT 6 cut(s) 105, 127, 301, 319, 560, 628
SsiI CCGC 4 cut(s) 204, 312, 330, 333
SspMI CTAG 5 cut(s) 12, 87, 293, 327, 539
StyD4I CCNGG 2 cut(s) 724, 741
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 576, 779
TaiI ACGT 2 cut(s) 436, 701
TaqI TCGA 3 cut(s) 83, 489, 519
TasI AATT 6 cut(s) 105, 127, 301, 319, 560, 628
TauI GCSGC 1 cut(s) 333
TfiI GAWTC 1 cut(s) 477
Tru1I TTAA 1 cut(s) 603
Tru9I TTAA 1 cut(s) 603
TscAI CASTG 3 cut(s) 142, 574, 699
TseFI GTSAC 5 cut(s) 70, 119, 218, 429, 694
TseI GCWGC 3 cut(s) 152, 542, 789
Tsp45I GTSAC 5 cut(s) 70, 119, 218, 429, 694
TspDTI ATGAA 1 cut(s) 651
TspRI CASTG 3 cut(s) 142, 574, 699
VpaK11BI GGWCC 1 cut(s) 674
XapI RAATTY 3 cut(s) 127, 560, 628
XceI RCATGY 1 cut(s) 772
XmiI GTMKAC 1 cut(s) 416
XspI CTAG 5 cut(s) 12, 87, 293, 327, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.