MD09G1021800.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002827
Physical Location & Seq
Forward (+)
1322237 .. 1324603
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1021800.v1.1.491

Sequence Viewer

Length: 1179 bp
ATGTTTCGTGTAGTGGAAATGCAGATGGCTAGATTCGTCGTTGCCGGTTGTTTCGCTATGTGTTTACTGAGTGTGGGAGGGGAAGGGGTGATAGACACATCGTCGCGTTGTGATTTTCCGGCAATTTACAACTTTGGAGACTCCAACTCAGATACTGGTGGTAACTCAGCTTCATTTTACCCCATGGTATCACCCTGTGGTGAAACTTTCTTCCACAGACCCGCCGGAAGGGGTTGTGACGGCCGTCTCATCATAGATTTTATTGCAAAGCATTTGGAGTTGCCATACTTGAGTCCATATTTGGACTCACTTGAGAGCAATTTCAGGCACGGTGCCAATTTCGCTAGCGGCGGATCTACCATCCGGCCTTACAAGGAGGCCATGTTTGAAAATGGTGTGAGCCCTTTTTATCTTGAAATCCAGATTGCTCAGTATCAGCAGTTCAAGTCACGTACCACAACTCTTTCCAAACAAGCCAAGAAACAATCCGACAGATTCCCAATAATCGATGACTTCTCAAAGGCGCTCTACACTTTCGATACAGGACAGAATGATCTAGCTGCTGGTTTTCGGACAAAGTTCAGCAGTGAACAATTTGAAGCAGAAATCACAGACATGATTAACCAGCATGCTAACGCCGTACGAAATTTGTATGAACAAGGGGCAAGGACATTCTGGATACACAACACGGGTCCGATTGGTTGCTTGGGAGTGACCTTAAGCTACCTCCATAATCCGAGCCCCGGCTTCATTGACAGCCAAGGTTGTGACAAGTTTCAGAACGACATGGCTAGGAAGTTCAATAGGCAGCTGGAGCAGAAAGTGATTCAGCTAAGGAAGGAGCTCCCCCTTTCTGCCATAACATATGTCGATATCTTCGCTGCAAAGTATGAACTGCTCAGCAACGCAAAGAAGCACGGATTTTTGGATAAGGCAAGCATCTGCTGTGGGTACCATGACGAAAACAACCATGTGTGGTGTGGGAATAAGGGCACCTTAACTAATGGGACTCAAGTATATGCTGGTTTATGTAAGGATCCTTCCTTGTATATCAGCTGGGATGGCGTACACTACACCGAGGCTGCGAACCGTTGGATTGCAGATCGAATAGTCCACGGTTCGTTCACAGACCCGCCGGTTTCGATCAAAAGTTCATGTCATACAACTCAACCTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

393

Amino Acids

43.81

Weight (kDa)

6.31

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 42 - 370 3.7e-47 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 951
AccB1I GGYRCC 3 cut(s) 332, 951, 992
AccII CGCG 1 cut(s) 106
AciI CCGC 4 cut(s) 222, 348, 351, 1133
AclWI GGATC 3 cut(s) 361, 1031, 1044
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 1 cut(s) 646
AdeI CACNNNGTG 1 cut(s) 197
AfaI GTAC 4 cut(s) 454, 642, 953, 1068
AfiI CCNNNNNNNGG 2 cut(s) 228, 743
AflII CTTAAG 1 cut(s) 718
AgsI TTSAA 5 cut(s) 389, 416, 445, 599, 802
AhdI GACNNNNNGTC 1 cut(s) 100
AluBI AGCT 7 cut(s) 170, 560, 723, 811, 832, 844, 1056
AluI AGCT 7 cut(s) 170, 560, 723, 811, 832, 844, 1056
Alw21I GWGCWC 1 cut(s) 846
Alw26I GTCTC 2 cut(s) 132, 251
AlwI GGATC 3 cut(s) 361, 1031, 1044
AlwNI CAGNNNCTG 1 cut(s) 155
AoxI GGCC 3 cut(s) 241, 365, 378
ApeKI GCWGC 4 cut(s) 560, 808, 881, 1082
ApoI RAATTY 1 cut(s) 646
Asp718I GGTACC 1 cut(s) 951
AspLEI GCGC 1 cut(s) 526
AspS9I GGNCC 1 cut(s) 692
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 3 cut(s) 100, 183, 212
AsuNHI GCTAGC 1 cut(s) 344
AvaII GGWCC 1 cut(s) 692
BaeGI GKGCMC 1 cut(s) 995
BamHI GGATCC 1 cut(s) 1036
BanI GGYRCC 3 cut(s) 332, 951, 992
BanII GRGCYC 3 cut(s) 404, 743, 846
BarI GAAGNNNNNNTAC 4 cut(s) 154, 186, 1024, 1056
Bbv12I GWGCWC 1 cut(s) 846
BbvI GCAGC 4 cut(s) 547, 820, 868, 1069
BccI CCATC 3 cut(s) 19, 368, 1055
BceAI ACGGC 3 cut(s) 228, 256, 623
BciVI GTATCC 1 cut(s) 672
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 2 cut(s) 132, 251
BfaI CTAG 4 cut(s) 30, 345, 557, 792
BfoI RGCGCY 1 cut(s) 527
BfrI CTTAAG 1 cut(s) 718
BfuI GTATCC 1 cut(s) 672
BisI GCNGC 5 cut(s) 349, 561, 809, 882, 1083
BlpI GCTNAGC 1 cut(s) 899
BlsI GCNGC 5 cut(s) 350, 562, 810, 883, 1084
Bme1390I CCNGG 1 cut(s) 744
Bme18I GGWCC 1 cut(s) 692
BmeRI GACNNNNNGTC 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 692
BmiI GGNNCC 5 cut(s) 334, 693, 953, 994, 1038
BmrFI CCNGG 1 cut(s) 744
BmsI GCATC 1 cut(s) 948
BmtI GCTAGC 1 cut(s) 348
BoxI GACNNNNGTC 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 833
Bpu10I CCTNAGC 1 cut(s) 833
Bpu1102I GCTNAGC 1 cut(s) 899
BpuEI CTTGAG 3 cut(s) 310, 332, 996
BpuMI CCSGG 1 cut(s) 744
Bsa29I ATCGAT 1 cut(s) 507
BsaAI YACGTR 1 cut(s) 452
BsaJI CCNNGG 5 cut(s) 183, 742, 760, 1077, 1114
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 743
Bse118I RCCGGY 2 cut(s) 44, 1135
Bse1I ACTGG 1 cut(s) 160
BseCI ATCGAT 1 cut(s) 507
BseDI CCNNGG 5 cut(s) 183, 742, 760, 1077, 1114
BseGI GGATG 2 cut(s) 360, 1066
BseLI CCNNNNNNNGG 2 cut(s) 228, 743
BseMII CTCAG 6 cut(s) 59, 162, 180, 443, 913, 1164
BseNI ACTGG 1 cut(s) 160
BseSI GKGCMC 1 cut(s) 995
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 4 cut(s) 547, 820, 868, 1069
BseYI CCCAGC 1 cut(s) 1056
Bsh1236I CGCG 1 cut(s) 106
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 3 cut(s) 243, 367, 380
BshNI GGYRCC 3 cut(s) 332, 951, 992
BshVI ATCGAT 1 cut(s) 507
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 846
BsiSI CCGG 6 cut(s) 45, 119, 225, 364, 744, 1136
BsiWI CGTACG 1 cut(s) 640
BslFI GGGAC 1 cut(s) 1021
BslI CCNNNNNNNGG 2 cut(s) 228, 743
BsmAI GTCTC 2 cut(s) 132, 251
BsmBI CGTCTC 1 cut(s) 251
BsmFI GGGAC 1 cut(s) 1021
BsnI GGCC 3 cut(s) 243, 367, 380
Bsp1286I GDGCHC 4 cut(s) 404, 743, 846, 995
Bsp143I GATC 5 cut(s) 353, 553, 1036, 1102, 1143
Bsp1720I GCTNAGC 1 cut(s) 899
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 4 cut(s) 222, 348, 351, 1133
BspANI GGCC 3 cut(s) 243, 367, 380
BspCNI CTCAG 6 cut(s) 60, 161, 179, 442, 912, 1165
BspDI ATCGAT 1 cut(s) 507
BspFNI CGCG 1 cut(s) 106
BspLI GGNNCC 5 cut(s) 334, 693, 953, 994, 1038
BspOI GCTAGC 1 cut(s) 348
BspPI GGATC 3 cut(s) 361, 1031, 1044
BspT107I GGYRCC 3 cut(s) 332, 951, 992
BspTI CTTAAG 1 cut(s) 718
BsrFI RCCGGY 2 cut(s) 44, 1135
BsrI ACTGG 1 cut(s) 160
BssAI RCCGGY 2 cut(s) 44, 1135
BssECI CCNNGG 5 cut(s) 183, 742, 760, 1077, 1114
BssMI GATC 5 cut(s) 353, 553, 1036, 1102, 1143
BssT1I CCWWGG 2 cut(s) 183, 760
Bst4CI ACNGT 3 cut(s) 332, 1091, 1118
BstAFI CTTAAG 1 cut(s) 718
BstBAI YACGTR 1 cut(s) 452
BstC8I GCNNGC 3 cut(s) 346, 630, 937
BstDEI CTNAG 7 cut(s) 68, 148, 166, 429, 833, 899, 1173
BstDSI CCRYGG 2 cut(s) 183, 1114
BstF5I GGATG 2 cut(s) 360, 1066
BstFNI CGCG 1 cut(s) 106
BstH2I RGCGCY 1 cut(s) 527
BstHHI GCGC 1 cut(s) 526
BstKTI GATC 5 cut(s) 356, 556, 1039, 1105, 1146
BstMAI GTCTC 2 cut(s) 132, 251
BstMBI GATC 5 cut(s) 353, 553, 1036, 1102, 1143
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 3 cut(s) 341, 814, 1062
BstNSI RCATGY 1 cut(s) 632
BstPAI GACNNNNGTC 1 cut(s) 243
BstSCI CCNGG 1 cut(s) 742
BstSLI GKGCMC 1 cut(s) 995
BstUI CGCG 1 cut(s) 106
BstV1I GCAGC 4 cut(s) 547, 820, 868, 1069
BstX2I RGATCY 2 cut(s) 353, 1036
BstYI RGATCY 2 cut(s) 353, 1036
BstZI CGGCCG 1 cut(s) 241
Bsu15I ATCGAT 1 cut(s) 507
BsuI GTATCC 1 cut(s) 672
BsuRI GGCC 3 cut(s) 243, 367, 380
BsuTUI ATCGAT 1 cut(s) 507
BtgI CCRYGG 2 cut(s) 183, 1114
BtsCI GGATG 2 cut(s) 360, 1066
BtsI GCAGTG 1 cut(s) 592
BtsIMutI CAGTG 1 cut(s) 592
Cac8I GCNNGC 3 cut(s) 346, 630, 937
CaiI CAGNNNCTG 1 cut(s) 155
CfoI GCGC 1 cut(s) 526
Cfr10I RCCGGY 2 cut(s) 44, 1135
Cfr13I GGNCC 1 cut(s) 692
ClaI ATCGAT 1 cut(s) 507
Csp6I GTAC 4 cut(s) 453, 641, 952, 1067
CviAII CATG 8 cut(s) 184, 382, 616, 629, 787, 956, 971, 1155
CviQI GTAC 4 cut(s) 453, 641, 952, 1067
DdeI CTNAG 7 cut(s) 68, 148, 166, 429, 833, 899, 1173
DpnI GATC 5 cut(s) 355, 555, 1038, 1104, 1145
DpnII GATC 5 cut(s) 353, 553, 1036, 1102, 1143
DraIII CACNNNGTG 1 cut(s) 197
DriI GACNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 241
EagI CGGCCG 1 cut(s) 241
Eam1105I GACNNNNNGTC 1 cut(s) 100
EciI GGCGGA 1 cut(s) 366
Ecl136II GAGCTC 1 cut(s) 844
EclXI CGGCCG 1 cut(s) 241
Eco130I CCWWGG 2 cut(s) 183, 760
Eco24I GRGCYC 3 cut(s) 404, 743, 846
Eco32I GATATC 1 cut(s) 874
Eco47I GGWCC 1 cut(s) 692
Eco52I CGGCCG 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 844
EcoICRI GAGCTC 1 cut(s) 844
EcoRV GATATC 1 cut(s) 874
EcoT14I CCWWGG 2 cut(s) 183, 760
EcoT38I GRGCYC 3 cut(s) 404, 743, 846
ErhI CCWWGG 2 cut(s) 183, 760
Esp3I CGTCTC 1 cut(s) 251
FaeI CATG 8 cut(s) 187, 385, 619, 632, 790, 959, 974, 1158
FalI AAGNNNNNCTT 2 cut(s) 980, 1012
FaqI GGGAC 1 cut(s) 1021
FatI CATG 8 cut(s) 183, 381, 615, 628, 786, 955, 970, 1154
FauI CCCGC 2 cut(s) 229, 1140
FauNDI CATATG 1 cut(s) 865
Fnu4HI GCNGC 5 cut(s) 349, 561, 809, 882, 1083
FokI GGATG 2 cut(s) 347, 1073
FriOI GRGCYC 3 cut(s) 404, 743, 846
Fsp4HI GCNGC 5 cut(s) 349, 561, 809, 882, 1083
FspBI CTAG 4 cut(s) 30, 345, 557, 792
GlaI GCGC 1 cut(s) 525
GluI GCNGC 5 cut(s) 349, 561, 809, 882, 1083
GsaI CCCAGC 1 cut(s) 1060
GsuI CTGGAG 1 cut(s) 833
HaeII RGCGCY 1 cut(s) 527
HaeIII GGCC 3 cut(s) 243, 367, 380
HapII CCGG 6 cut(s) 45, 119, 225, 364, 744, 1136
HhaI GCGC 1 cut(s) 526
Hin1II CATG 8 cut(s) 187, 385, 619, 632, 790, 959, 974, 1158
Hin6I GCGC 1 cut(s) 524
HinP1I GCGC 1 cut(s) 524
HinfI GANTC 7 cut(s) 33, 140, 292, 305, 495, 826, 1009
HpaII CCGG 6 cut(s) 45, 119, 225, 364, 744, 1136
HphI GGTGA 3 cut(s) 100, 183, 212
Hpy166II GTNNAC 5 cut(s) 65, 590, 1069, 1114, 1125
Hpy188I TCNGA 6 cut(s) 151, 490, 573, 696, 738, 780
Hpy188III TCNNGA 3 cut(s) 413, 421, 676
Hpy8I GTNNAC 5 cut(s) 65, 590, 1069, 1114, 1125
Hpy99I CGWCG 2 cut(s) 41, 106
HpyAV CCTTC 4 cut(s) 77, 222, 832, 1050
HpyCH4III ACNGT 3 cut(s) 332, 1091, 1118
HpyCH4IV ACGT 1 cut(s) 451
HpyCH4V TGCA 4 cut(s) 22, 266, 884, 1100
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 814, 1062
HpyF3I CTNAG 7 cut(s) 68, 148, 166, 429, 833, 899, 1173
HpySE526I ACGT 1 cut(s) 451
Hsp92II CATG 8 cut(s) 187, 385, 619, 632, 790, 959, 974, 1158
HspAI GCGC 1 cut(s) 524
KpnI GGTACC 1 cut(s) 955
Kzo9I GATC 5 cut(s) 353, 553, 1036, 1102, 1143
LmnI GCTCC 3 cut(s) 814, 841, 849
Lsp1109I GCAGC 4 cut(s) 547, 820, 868, 1069
LweI GCATC 1 cut(s) 948
MaeI CTAG 4 cut(s) 30, 345, 557, 792
MaeII ACGT 1 cut(s) 451
MaeIII GTNAC 5 cut(s) 161, 236, 447, 712, 767
MalI GATC 5 cut(s) 355, 555, 1038, 1104, 1145
MboI GATC 5 cut(s) 353, 553, 1036, 1102, 1143
MboII GAAGA 2 cut(s) 202, 868
MflI RGATCY 2 cut(s) 353, 1036
MhlI GDGCHC 4 cut(s) 404, 743, 846, 995
MluCI AATT 5 cut(s) 123, 319, 337, 593, 646
MlyI GAGTC 4 cut(s) 134, 299, 301, 1003
MmeI TCCRAC 3 cut(s) 168, 513, 1073
MnlI CCTC 4 cut(s) 71, 370, 737, 1072
MseI TTAA 3 cut(s) 621, 719, 998
MslI CAYNNNNRTG 1 cut(s) 614
MspA1I CMGCKG 2 cut(s) 811, 1056
MspCI CTTAAG 1 cut(s) 718
MspI CCGG 6 cut(s) 45, 119, 225, 364, 744, 1136
MspR9I CCNGG 1 cut(s) 744
MvnI CGCG 1 cut(s) 106
MwoI GCNNNNNNNGC 3 cut(s) 341, 814, 1062
NciI CCSGG 1 cut(s) 744
NcoI CCATGG 1 cut(s) 183
NdeI CATATG 1 cut(s) 865
NdeII GATC 5 cut(s) 353, 553, 1036, 1102, 1143
NheI GCTAGC 1 cut(s) 344
NlaIII CATG 8 cut(s) 187, 385, 619, 632, 790, 959, 974, 1158
NlaIV GGNNCC 5 cut(s) 334, 693, 953, 994, 1038
NmuCI GTSAC 4 cut(s) 236, 447, 712, 767
NspI RCATGY 1 cut(s) 632
PaeI GCATGC 1 cut(s) 632
PfeI GAWTC 3 cut(s) 33, 495, 826
Pfl23II CGTACG 1 cut(s) 640
PkrI GCNGC 5 cut(s) 350, 562, 810, 883, 1084
PleI GAGTC 4 cut(s) 134, 299, 300, 1003
PpsI GAGTC 4 cut(s) 134, 299, 300, 1003
Ppu21I YACGTR 1 cut(s) 452
PshAI GACNNNNGTC 1 cut(s) 243
Psp124BI GAGCTC 1 cut(s) 846
PspFI CCCAGC 1 cut(s) 1056
PspLI CGTACG 1 cut(s) 640
PspN4I GGNNCC 5 cut(s) 334, 693, 953, 994, 1038
PspPI GGNCC 1 cut(s) 692
PsrI GAACNNNNNNTAC 2 cut(s) 425, 457
PstNI CAGNNNCTG 1 cut(s) 155
PsuI RGATCY 2 cut(s) 353, 1036
PvuII CAGCTG 2 cut(s) 811, 1056
RsaI GTAC 4 cut(s) 454, 642, 953, 1068
RsaNI GTAC 4 cut(s) 453, 641, 952, 1067
RseI CAYNNNNRTG 1 cut(s) 614
SacI GAGCTC 1 cut(s) 846
SaqAI TTAA 3 cut(s) 621, 719, 998
SatI GCNGC 5 cut(s) 349, 561, 809, 882, 1083
Sau3AI GATC 5 cut(s) 353, 553, 1036, 1102, 1143
Sau96I GGNCC 1 cut(s) 692
SchI GAGTC 4 cut(s) 134, 299, 301, 1003
ScrFI CCNGG 1 cut(s) 744
SduI GDGCHC 4 cut(s) 404, 743, 846, 995
SfaNI GCATC 1 cut(s) 948
SinI GGWCC 1 cut(s) 692
SmiMI CAYNNNNRTG 1 cut(s) 614
SmlI CTYRAG 4 cut(s) 289, 311, 718, 1011
SmoI CTYRAG 4 cut(s) 289, 311, 718, 1011
SphI GCATGC 1 cut(s) 632
Sse9I AATT 5 cut(s) 123, 319, 337, 593, 646
SsiI CCGC 4 cut(s) 222, 348, 351, 1133
SspMI CTAG 4 cut(s) 30, 345, 557, 792
SstI GAGCTC 1 cut(s) 846
StyD4I CCNGG 1 cut(s) 742
StyI CCWWGG 2 cut(s) 183, 760
TaaI ACNGT 3 cut(s) 332, 1091, 1118
TaiI ACGT 1 cut(s) 454
TaqI TCGA 5 cut(s) 507, 537, 870, 1105, 1142
TasI AATT 5 cut(s) 123, 319, 337, 593, 646
TauI GCSGC 1 cut(s) 351
TfiI GAWTC 3 cut(s) 33, 495, 826
Tru1I TTAA 3 cut(s) 621, 719, 998
Tru9I TTAA 3 cut(s) 621, 719, 998
TscAI CASTG 1 cut(s) 592
TseFI GTSAC 4 cut(s) 236, 447, 712, 767
TseI GCWGC 4 cut(s) 560, 808, 881, 1082
Tsp45I GTSAC 4 cut(s) 236, 447, 712, 767
TspDTI ATGAA 5 cut(s) 162, 669, 739, 906, 1143
TspGWI ACGGA 1 cut(s) 933
TspRI CASTG 1 cut(s) 592
Vha464I CTTAAG 1 cut(s) 718
VpaK11BI GGWCC 1 cut(s) 692
XapI RAATTY 1 cut(s) 646
XceI RCATGY 1 cut(s) 632
XcmI CCANNNNNNNNNTGG 1 cut(s) 977
XspI CTAG 4 cut(s) 30, 345, 557, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.