FvH4_6g51900

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002827
Physical Location & Seq
Reverse (-)
38398258 .. 38400324
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g51900.t1

Sequence Viewer

Length: 1173 bp
ATGTTGGGAATGGAGAGATGGAAACAACTTGTGCTACTGCCTTTTGGGTTGTTCTTGGCAATGTGTATTCTGGGAGTCAGTGGTAGGGCGTTACCATCTTGTTATTTTCCGGCAATCTACAACTTTGGTGACTCGAACTCCGACACCGGCGGGATCTCAGCTGCATTTGAACCCATTCAGGCACCCTACGGCGAGGGGTTCTTTGGTAAACCAGCTGGAAGGGACTCCGATGGTCGTCTCATGATAGACTTCTTTGCTGAGCACCTAAGGTTGCCATACGTGACTGCATACTTGAACTCGCTGGGAACAAGTTTCAGGTACGGTGCAAATTTTGCCACCGGAGGATCACCCATTAGAAGGCCGAAAGGGACCATTTTCGAGACCATTTTCGGTTTTGGCCTTAGCCCCTTTTCGCTTGATATGCAAACGACTCAGTTCCTGCAATTCAAAGCTCGATCGGCTGATCTATTTCAAAAAGCCAATTATTTTTGTGACAGAAGCAAACTTCCAAATCCTCAAGACTTTGCAAAGGCACTGTATACGTTTGATATTGGGCAGAATGATCTCTCTGCTGGTTTTCGAAAGTTGAGCTTTGATCAGCTTCGTGCAGAAATTCCAGACATGATAAACCAGCATGCCGCAGCAGTCCGACTTGTATACGAACAAGGGGGGAGGGCATTTTGGATACACAACGCCGGTCCGATTGGTTGCTTGCCGGTGGGTTTGTTCTACAATATCAAACCGGCACCGAGTGCTCTCGATGAACACGGCTGTGTTAAGGACCAAAACGAGATGGCCATAGAATTCAACAGGCAGCTCAAGGACAGTGTCATCAAACTAAGAGCTGAGCTCCCCCAAGCTGCAATCACATATGTAGATATCTATGCTGCCAAGTATGAACTCATCAGCAATGCAAAAAAAGAAGGGTTTATGGCTCCAATGAAGGTTTGCTGTGGGTTTCATATGGGAAATGACCATGTGTGGTGTGGAAACAAAGCAACTGTAAATGGAACTGAAGTATTTGGTGGTGCTTGTGGAAATGCTTCATCTGTTATTAGCTGGGATGGAATCCACTACACTCAGGCTGCTAATAAGTGGGTTGCCAATCGCATACTTAATGGTGCCTTGTCCGATCCACCACTTCCCATTTCTCAAGCTTGTCATAGGCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

391

Amino Acids

42.96

Weight (kDa)

8.26

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 39 - 371 1e-41 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 181, 745, 1121
AccI GTMKAC 2 cut(s) 539, 657
AciI CCGC 2 cut(s) 150, 639
AclWI GGATC 3 cut(s) 161, 352, 1127
AcoI YGGCCR 1 cut(s) 795
AcsI RAATTY 3 cut(s) 328, 612, 803
AcuI CTGAAG 1 cut(s) 1035
AdeI CACNNNGTG 1 cut(s) 752
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 1 cut(s) 357
AgsI TTSAA 5 cut(s) 170, 295, 448, 473, 808
AleI CACNNNNGTG 1 cut(s) 771
Alw21I GWGCWC 3 cut(s) 264, 757, 852
Alw26I GTCTC 2 cut(s) 242, 374
AlwI GGATC 3 cut(s) 161, 352, 1127
AlwNI CAGNNNCTG 1 cut(s) 439
AoxI GGCC 3 cut(s) 359, 397, 795
ApeKI GCWGC 6 cut(s) 161, 641, 814, 860, 887, 1085
ApoI RAATTY 3 cut(s) 328, 612, 803
Asp700I GAANNNNTTC 2 cut(s) 174, 1042
AspLEI GCGC 1 cut(s) 1170
AspS9I GGNCC 3 cut(s) 369, 698, 781
AsuHPI GGTGA 2 cut(s) 140, 339
AsuII TTCGAA 1 cut(s) 580
AvaII GGWCC 3 cut(s) 369, 698, 781
AxyI CCTNAGG 1 cut(s) 266
BalI TGGCCA 1 cut(s) 797
BanI GGYRCC 3 cut(s) 181, 745, 1121
BanII GRGCYC 1 cut(s) 852
Bbv12I GWGCWC 3 cut(s) 264, 757, 852
BbvI GCAGC 6 cut(s) 148, 653, 826, 847, 874, 1072
BccI CCATC 5 cut(s) 12, 103, 224, 787, 1058
BceAI ACGGC 2 cut(s) 205, 784
BciVI GTATCC 1 cut(s) 678
BclI TGATCA 1 cut(s) 595
BcoDI GTCTC 2 cut(s) 242, 374
BfoI RGCGCY 1 cut(s) 1171
BfuI GTATCC 1 cut(s) 678
BisI GCNGC 7 cut(s) 162, 639, 642, 815, 861, 888, 1086
BlpI GCTNAGC 2 cut(s) 258, 846
BlsI GCNGC 7 cut(s) 163, 640, 643, 816, 862, 889, 1087
Bme18I GGWCC 3 cut(s) 369, 698, 781
BmgT120I GGNCC 3 cut(s) 369, 698, 781
BmiI GGNNCC 5 cut(s) 183, 370, 747, 936, 1123
BplI GAGNNNNNCTC 2 cut(s) 834, 866
Bpu10I CCTNAGC 1 cut(s) 401
Bpu1102I GCTNAGC 2 cut(s) 258, 846
Bpu14I TTCGAA 1 cut(s) 580
BpuEI CTTGAG 3 cut(s) 501, 803, 1137
BsaAI YACGTR 1 cut(s) 280
BsaI GGTCTC 1 cut(s) 374
BsaWI WCCGGW 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 1 cut(s) 357
Bse118I RCCGGY 4 cut(s) 146, 695, 715, 742
Bse21I CCTNAGG 1 cut(s) 266
Bse3DI GCAATG 2 cut(s) 66, 916
BseGI GGATG 1 cut(s) 1069
BseLI CCNNNNNNNGG 1 cut(s) 357
BseMI GCAATG 2 cut(s) 66, 916
BseMII CTCAG 5 cut(s) 171, 249, 446, 837, 1094
BseXI GCAGC 6 cut(s) 148, 653, 826, 847, 874, 1072
BseYI CCCAGC 2 cut(s) 301, 1059
BsgI GTGCAG 1 cut(s) 627
Bsh1285I CGRYCG 1 cut(s) 458
BshFI GGCC 3 cut(s) 361, 399, 797
BshNI GGYRCC 3 cut(s) 181, 745, 1121
BsiEI CGRYCG 1 cut(s) 458
BsiHKAI GWGCWC 3 cut(s) 264, 757, 852
BsiSI CCGG 6 cut(s) 110, 147, 339, 696, 716, 743
BslFI GGGAC 2 cut(s) 236, 382
BslI CCNNNNNNNGG 1 cut(s) 357
BsmAI GTCTC 2 cut(s) 242, 374
BsmBI CGTCTC 1 cut(s) 242
BsmFI GGGAC 2 cut(s) 236, 382
BsnI GGCC 3 cut(s) 361, 399, 797
Bso31I GGTCTC 1 cut(s) 374
Bsp119I TTCGAA 1 cut(s) 580
Bsp1286I GDGCHC 3 cut(s) 264, 757, 852
Bsp143I GATC 7 cut(s) 153, 344, 455, 463, 562, 595, 1132
Bsp1720I GCTNAGC 2 cut(s) 258, 846
BspACI CCGC 2 cut(s) 150, 639
BspANI GGCC 3 cut(s) 361, 399, 797
BspCNI CTCAG 5 cut(s) 170, 250, 445, 838, 1093
BspHI TCATGA 1 cut(s) 240
BspLI GGNNCC 5 cut(s) 183, 370, 747, 936, 1123
BspPI GGATC 3 cut(s) 161, 352, 1127
BspT104I TTCGAA 1 cut(s) 580
BspT107I GGYRCC 3 cut(s) 181, 745, 1121
BspTNI GGTCTC 1 cut(s) 374
BsrDI GCAATG 2 cut(s) 66, 916
BsrFI RCCGGY 4 cut(s) 146, 695, 715, 742
BssAI RCCGGY 4 cut(s) 146, 695, 715, 742
BssMI GATC 7 cut(s) 153, 344, 455, 463, 562, 595, 1132
BssNAI GTATAC 2 cut(s) 540, 658
Bst1107I GTATAC 2 cut(s) 540, 658
Bst4CI ACNGT 4 cut(s) 323, 537, 827, 1003
BstAPI GCANNNNNTGC 2 cut(s) 332, 752
BstBAI YACGTR 1 cut(s) 280
BstBI TTCGAA 1 cut(s) 580
BstC8I GCNNGC 2 cut(s) 636, 713
BstDEI CTNAG 8 cut(s) 157, 258, 266, 401, 432, 839, 846, 1080
BstF5I GGATG 1 cut(s) 1069
BstH2I RGCGCY 1 cut(s) 1171
BstHHI GCGC 1 cut(s) 1170
BstKTI GATC 7 cut(s) 156, 347, 458, 466, 565, 598, 1135
BstMAI GTCTC 2 cut(s) 242, 374
BstMBI GATC 7 cut(s) 153, 344, 455, 463, 562, 595, 1132
BstMCI CGRYCG 1 cut(s) 458
BstMWI GCNNNNNNNGC 4 cut(s) 332, 421, 458, 752
BstNSI RCATGY 1 cut(s) 638
BstV1I GCAGC 6 cut(s) 148, 653, 826, 847, 874, 1072
BstX2I RGATCY 1 cut(s) 153
BstYI RGATCY 1 cut(s) 153
BstZ17I GTATAC 2 cut(s) 540, 658
Bsu36I CCTNAGG 1 cut(s) 266
BsuI GTATCC 1 cut(s) 678
BsuRI GGCC 3 cut(s) 361, 399, 797
BtsCI GGATG 1 cut(s) 1069
BtsIMutI CAGTG 3 cut(s) 85, 533, 832
Cac8I GCNNGC 2 cut(s) 636, 713
CaiI CAGNNNCTG 1 cut(s) 439
CciI TCATGA 1 cut(s) 240
CfoI GCGC 1 cut(s) 1170
Cfr10I RCCGGY 4 cut(s) 146, 695, 715, 742
Cfr13I GGNCC 3 cut(s) 369, 698, 781
CpoI CGGWCCG 1 cut(s) 698
Csp6I GTAC 1 cut(s) 319
CspI CGGWCCG 1 cut(s) 698
CviAII CATG 4 cut(s) 241, 622, 635, 977
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 8 cut(s) 157, 258, 266, 401, 432, 839, 846, 1080
DpnI GATC 7 cut(s) 155, 346, 457, 465, 564, 597, 1134
DpnII GATC 7 cut(s) 153, 344, 455, 463, 562, 595, 1132
DraIII CACNNNGTG 1 cut(s) 752
EaeI YGGCCR 1 cut(s) 795
Ecl136II GAGCTC 1 cut(s) 850
Eco24I GRGCYC 1 cut(s) 852
Eco31I GGTCTC 1 cut(s) 374
Eco32I GATATC 1 cut(s) 880
Eco47I GGWCC 3 cut(s) 369, 698, 781
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 1 cut(s) 1035
Eco81I CCTNAGG 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 850
EcoRI GAATTC 1 cut(s) 803
EcoRV GATATC 1 cut(s) 880
EcoT38I GRGCYC 1 cut(s) 852
Esp3I CGTCTC 1 cut(s) 242
FaeI CATG 4 cut(s) 244, 625, 638, 980
FalI AAGNNNNNCTT 2 cut(s) 575, 607
FaqI GGGAC 2 cut(s) 236, 382
FatI CATG 4 cut(s) 240, 621, 634, 976
FauI CCCGC 1 cut(s) 143
FauNDI CATATG 2 cut(s) 871, 963
FbaI TGATCA 1 cut(s) 595
FblI GTMKAC 2 cut(s) 539, 657
Fnu4HI GCNGC 7 cut(s) 162, 639, 642, 815, 861, 888, 1086
FokI GGATG 1 cut(s) 1076
FriOI GRGCYC 1 cut(s) 852
Fsp4HI GCNGC 7 cut(s) 162, 639, 642, 815, 861, 888, 1086
GlaI GCGC 1 cut(s) 1169
GluI GCNGC 7 cut(s) 162, 639, 642, 815, 861, 888, 1086
GsaI CCCAGC 2 cut(s) 305, 1063
HaeII RGCGCY 1 cut(s) 1171
HaeIII GGCC 3 cut(s) 361, 399, 797
HapII CCGG 6 cut(s) 110, 147, 339, 696, 716, 743
HhaI GCGC 1 cut(s) 1170
Hin1II CATG 4 cut(s) 244, 625, 638, 980
Hin6I GCGC 1 cut(s) 1168
HinP1I GCGC 1 cut(s) 1168
HindIII AAGCTT 1 cut(s) 1155
HinfI GANTC 5 cut(s) 75, 131, 224, 430, 1068
HpaII CCGG 6 cut(s) 110, 147, 339, 696, 716, 743
HphI GGTGA 2 cut(s) 140, 339
Hpy166II GTNNAC 3 cut(s) 209, 540, 658
Hpy188I TCNGA 5 cut(s) 142, 229, 650, 702, 1132
Hpy188III TCNNGA 5 cut(s) 241, 379, 518, 617, 758
Hpy8I GTNNAC 3 cut(s) 209, 540, 658
HpyAV CCTTC 4 cut(s) 213, 351, 917, 937
HpyCH4III ACNGT 4 cut(s) 323, 537, 827, 1003
HpyCH4IV ACGT 2 cut(s) 279, 542
HpyCH4V TGCA 9 cut(s) 164, 287, 326, 424, 442, 527, 608, 863, 914
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 421, 458, 752
HpyF3I CTNAG 8 cut(s) 157, 258, 266, 401, 432, 839, 846, 1080
HpySE526I ACGT 2 cut(s) 279, 542
Hsp92II CATG 4 cut(s) 244, 625, 638, 980
HspAI GCGC 1 cut(s) 1168
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 7 cut(s) 153, 344, 455, 463, 562, 595, 1132
LmnI GCTCC 2 cut(s) 855, 940
Lsp1109I GCAGC 6 cut(s) 148, 653, 826, 847, 874, 1072
MaeII ACGT 2 cut(s) 279, 542
MaeIII GTNAC 4 cut(s) 90, 128, 280, 491
MalI GATC 7 cut(s) 155, 346, 457, 465, 564, 597, 1134
MboI GATC 7 cut(s) 153, 344, 455, 463, 562, 595, 1132
MflI RGATCY 1 cut(s) 153
MhlI GDGCHC 3 cut(s) 264, 757, 852
MlsI TGGCCA 1 cut(s) 797
MluCI AATT 5 cut(s) 328, 443, 481, 612, 803
MluNI TGGCCA 1 cut(s) 797
MlyI GAGTC 4 cut(s) 84, 125, 218, 424
MmeI TCCRAC 2 cut(s) 165, 673
MnlI CCTC 4 cut(s) 187, 335, 525, 666
Mox20I TGGCCA 1 cut(s) 797
MroXI GAANNNNTTC 2 cut(s) 174, 1042
MscI TGGCCA 1 cut(s) 797
MseI TTAA 2 cut(s) 777, 1116
MslI CAYNNNNRTG 1 cut(s) 771
Msp20I TGGCCA 1 cut(s) 797
MspA1I CMGCKG 2 cut(s) 161, 215
MspI CCGG 6 cut(s) 110, 147, 339, 696, 716, 743
MwoI GCNNNNNNNGC 4 cut(s) 332, 421, 458, 752
NdeI CATATG 2 cut(s) 871, 963
NdeII GATC 7 cut(s) 153, 344, 455, 463, 562, 595, 1132
NlaIII CATG 4 cut(s) 244, 625, 638, 980
NlaIV GGNNCC 5 cut(s) 183, 370, 747, 936, 1123
NmuCI GTSAC 3 cut(s) 128, 280, 491
NspI RCATGY 1 cut(s) 638
NspV TTCGAA 1 cut(s) 580
OliI CACNNNNGTG 1 cut(s) 771
PaeI GCATGC 1 cut(s) 638
PagI TCATGA 1 cut(s) 240
PdmI GAANNNNTTC 2 cut(s) 174, 1042
PfeI GAWTC 1 cut(s) 1068
PflFI GACNNNGTC 1 cut(s) 827
PkrI GCNGC 7 cut(s) 163, 640, 643, 816, 862, 889, 1087
Ple19I CGATCG 1 cut(s) 458
PleI GAGTC 4 cut(s) 83, 125, 218, 424
PpsI GAGTC 4 cut(s) 83, 125, 218, 424
Ppu21I YACGTR 1 cut(s) 280
Psp124BI GAGCTC 1 cut(s) 852
PspFI CCCAGC 2 cut(s) 301, 1059
PspN4I GGNNCC 5 cut(s) 183, 370, 747, 936, 1123
PspPI GGNCC 3 cut(s) 369, 698, 781
PstNI CAGNNNCTG 1 cut(s) 439
PsuI RGATCY 1 cut(s) 153
PsyI GACNNNGTC 1 cut(s) 827
PvuI CGATCG 1 cut(s) 458
PvuII CAGCTG 2 cut(s) 161, 215
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
RseI CAYNNNNRTG 1 cut(s) 771
Rsr2I CGGWCCG 1 cut(s) 698
RsrII CGGWCCG 1 cut(s) 698
SacI GAGCTC 1 cut(s) 852
SaqAI TTAA 2 cut(s) 777, 1116
SatI GCNGC 7 cut(s) 162, 639, 642, 815, 861, 888, 1086
Sau3AI GATC 7 cut(s) 153, 344, 455, 463, 562, 595, 1132
Sau96I GGNCC 3 cut(s) 369, 698, 781
SchI GAGTC 4 cut(s) 84, 125, 218, 424
SduI GDGCHC 3 cut(s) 264, 757, 852
SfuI TTCGAA 1 cut(s) 580
SgrAI CRCCGGYG 1 cut(s) 146
SinI GGWCC 3 cut(s) 369, 698, 781
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 3 cut(s) 516, 818, 1152
SmoI CTYRAG 3 cut(s) 516, 818, 1152
SphI GCATGC 1 cut(s) 638
Sse9I AATT 5 cut(s) 328, 443, 481, 612, 803
SsiI CCGC 2 cut(s) 150, 639
SstI GAGCTC 1 cut(s) 852
TaaI ACNGT 4 cut(s) 323, 537, 827, 1003
TaiI ACGT 2 cut(s) 282, 545
TaqI TCGA 5 cut(s) 134, 378, 454, 580, 759
TasI AATT 5 cut(s) 328, 443, 481, 612, 803
TauI GCSGC 1 cut(s) 641
TfiI GAWTC 1 cut(s) 1068
Tru1I TTAA 2 cut(s) 777, 1116
Tru9I TTAA 2 cut(s) 777, 1116
TscAI CASTG 3 cut(s) 85, 540, 832
TseFI GTSAC 3 cut(s) 128, 280, 491
TseI GCWGC 6 cut(s) 161, 641, 814, 860, 887, 1085
Tsp45I GTSAC 3 cut(s) 128, 280, 491
TspDTI ATGAA 5 cut(s) 777, 912, 950, 956, 1035
TspRI CASTG 3 cut(s) 85, 540, 832
Tth111I GACNNNGTC 1 cut(s) 827
VpaK11BI GGWCC 3 cut(s) 369, 698, 781
XapI RAATTY 3 cut(s) 328, 612, 803
XceI RCATGY 1 cut(s) 638
XcmI CCANNNNNNNNNTGG 1 cut(s) 983
XmiI GTMKAC 2 cut(s) 539, 657
XmnI GAANNNNTTC 2 cut(s) 174, 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.