Rh2CG624300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002827
Physical Location & Seq
Reverse (-)
79886452 .. 79887962
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG624300.1

Sequence Viewer

Length: 1164 bp
ATGGAGATGGAAAGATTTTTGGTTGTGAGCATTTTGGTAACATGTGTGCTAGTAATCAGTGTGGGAGGTAAAGAAGATGCATCGTCACCTTCGGCTTCAGTTTCGGCTTGTGAGTTTCCAGCAATCTATAACTTTGGAGACTCGAATTCAGATACCGGTGGTATATCCGCTGCATTCTACGCAATGGCATCACCCTACGGTGAAACTTTCTTCCACAGACCAGCTGGAAGGGCCAGTGATGGTCGTCTAATAATAGATTTTATAGCCGAGAAGCTGGGGTTGCCATACCTGAGTTCATATTTGGACTCAATTCGGAGCAATTTCAGACACGGTGCCAATTTTGCTTCAGGAGGAGCAACCATCAGGCGGCAGAATGAGTCATGGTTTGAGAATGGTGTGAGTCCTTTTCCCCTTGACATCCAGTTTGTGCAGTATGATCAGTTCAAGGCGCGTACCACCAACCAAGCTAAGGATCTTTCTGACATACAAGATCTACCCAGAGCAGAAGACTTCACAAAGGCTCTTTACACATTTGATATAGGCCAAAATGATCTAGCTGCTGGTTTTCGGAAAATGAGTACCGAGCAATTTCTAGCAGAAATTCCAGACATTGTTAACCAGTTGGCCACAGCCATCCGAAGTTTGTATGACCGAGGGGCAAGGGCATTCTGGATACACAACACTGGTCCAATAGGTTGCTTGGGAGTGACATTACACTACATCCATGACCCGAGCCCTCCCGGATACCTTGATCGCCACGGTTGCGTGAAGTTTCAAAACCACATGGCGAGACAGTTCAATAGGCTGCTCAAACAGAAAGTGATCCAACTAAGGAAGGAACTCCCTCTTTCTGCAATAACTTATGTCAATGTCTTTGCAGCTAAGTATAAGCTCATCAGCAATGCAAAGAAGCTTGGTTTTTTCGATAAAACCAAGATCTGCTGTGGATATCATGAAGATTATGACCATGTGTGGTGTGGTAATAAGGGAAAGATAAATGGGAGTGAAACATATGCAGGTTCATGTGAAGATCCTTCCCTGTATATAAGCTGGGATGGCGTGCATTATAGTGAGGCTGCAAATCATTGGATTGCTAATCAGATTGCCAGTGGTTCATTCTCAGATCCACCGGTTTCAATCATAAATGCATGTCATAGGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

387

Amino Acids

43.0

Weight (kDa)

6.46

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 42 - 368 4.6e-45 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1007
AccB1I GGYRCC 1 cut(s) 332
AccII CGCG 1 cut(s) 451
AciI CCGC 2 cut(s) 168, 367
AclWI GGATC 4 cut(s) 480, 817, 1025, 1118
AcoI YGGCCR 1 cut(s) 624
AcsI RAATTY 2 cut(s) 145, 600
AcuI CTGAAG 2 cut(s) 81, 330
AfaI GTAC 2 cut(s) 454, 580
AfiI CCNNNNNNNGG 2 cut(s) 366, 469
AflIII ACRYGT 1 cut(s) 41
AgeI ACCGGT 2 cut(s) 155, 1129
AgsI TTSAA 4 cut(s) 445, 776, 799, 1137
AluBI AGCT 8 cut(s) 224, 274, 467, 557, 881, 892, 913, 1050
AluI AGCT 8 cut(s) 224, 274, 467, 557, 881, 892, 913, 1050
Alw26I GTCTC 2 cut(s) 132, 784
AlwI GGATC 4 cut(s) 480, 817, 1025, 1118
Ama87I CYCGRG 1 cut(s) 730
AoxI GGCC 4 cut(s) 231, 541, 624, 1157
ApeKI GCWGC 5 cut(s) 170, 557, 805, 878, 1076
ApoI RAATTY 2 cut(s) 145, 600
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
AsiGI ACCGGT 2 cut(s) 155, 1129
AspLEI GCGC 1 cut(s) 451
AspS9I GGNCC 2 cut(s) 231, 686
AsuC2I CCSGG 1 cut(s) 741
AsuHPI GGTGA 3 cut(s) 78, 183, 212
AvaI CYCGRG 1 cut(s) 730
AvaII GGWCC 1 cut(s) 686
BalI TGGCCA 1 cut(s) 626
BanI GGYRCC 1 cut(s) 332
BanII GRGCYC 1 cut(s) 737
BbsI GAAGAC 1 cut(s) 513
BbvI GCAGC 5 cut(s) 157, 544, 792, 890, 1063
BccI CCATC 4 cut(s) 233, 368, 641, 1049
BciVI GTATCC 2 cut(s) 666, 737
BclI TGATCA 1 cut(s) 436
BcnI CCSGG 1 cut(s) 741
BcoDI GTCTC 2 cut(s) 132, 784
BfaI CTAG 3 cut(s) 50, 554, 593
BfuAI ACCTGC 1 cut(s) 1007
BfuI GTATCC 2 cut(s) 666, 737
BglII AGATCT 2 cut(s) 490, 936
BisI GCNGC 6 cut(s) 171, 368, 558, 806, 879, 1077
BlsI GCNGC 6 cut(s) 172, 369, 559, 807, 880, 1078
Bme1390I CCNGG 1 cut(s) 741
Bme18I GGWCC 1 cut(s) 686
BmeT110I CYCGRG 1 cut(s) 730
BmgT120I GGNCC 2 cut(s) 231, 686
BmiI GGNNCC 1 cut(s) 334
BmrFI CCNGG 1 cut(s) 741
BmsI GCATC 3 cut(s) 67, 89, 197
BpiI GAAGAC 1 cut(s) 513
Bpu10I CCTNAGC 1 cut(s) 468
BpuMI CCSGG 1 cut(s) 741
BsaJI CCNNGG 2 cut(s) 652, 757
BsaWI WCCGGW 2 cut(s) 155, 1129
Bsc4I CCNNNNNNNGG 2 cut(s) 366, 469
Bse118I RCCGGY 2 cut(s) 155, 1129
Bse1I ACTGG 5 cut(s) 234, 421, 619, 688, 1107
Bse3DI GCAATG 2 cut(s) 189, 907
BseDI CCNNGG 2 cut(s) 652, 757
BseGI GGATG 4 cut(s) 417, 633, 720, 1060
BseLI CCNNNNNNNGG 2 cut(s) 366, 469
BseMI GCAATG 2 cut(s) 189, 907
BseMII CTCAG 2 cut(s) 281, 1134
BseNI ACTGG 5 cut(s) 234, 421, 619, 688, 1107
BseRI GAGGAG 1 cut(s) 366
BseXI GCAGC 5 cut(s) 157, 544, 792, 890, 1063
BseYI CCCAGC 2 cut(s) 274, 1050
BsgI GTGCAG 1 cut(s) 449
Bsh1236I CGCG 1 cut(s) 451
BshFI GGCC 4 cut(s) 233, 543, 626, 1159
BshNI GGYRCC 1 cut(s) 332
BshTI ACCGGT 2 cut(s) 155, 1129
BsiHKCI CYCGRG 1 cut(s) 730
BsiSI CCGG 3 cut(s) 156, 741, 1130
BslI CCNNNNNNNGG 2 cut(s) 366, 469
BsmAI GTCTC 2 cut(s) 132, 784
BsmI GAATGC 2 cut(s) 173, 665
BsnI GGCC 4 cut(s) 233, 543, 626, 1159
BsoBI CYCGRG 1 cut(s) 730
Bsp1286I GDGCHC 1 cut(s) 737
Bsp143I GATC 9 cut(s) 436, 472, 490, 550, 751, 822, 936, 1030, 1123
BspACI CCGC 2 cut(s) 168, 367
BspANI GGCC 4 cut(s) 233, 543, 626, 1159
BspCNI CTCAG 2 cut(s) 282, 1133
BspFNI CGCG 1 cut(s) 451
BspHI TCATGA 1 cut(s) 952
BspLI GGNNCC 1 cut(s) 334
BspMI ACCTGC 1 cut(s) 1007
BspPI GGATC 4 cut(s) 480, 817, 1025, 1118
BspT107I GGYRCC 1 cut(s) 332
BsrDI GCAATG 2 cut(s) 189, 907
BsrFI RCCGGY 2 cut(s) 155, 1129
BsrI ACTGG 5 cut(s) 234, 421, 619, 688, 1107
BssAI RCCGGY 2 cut(s) 155, 1129
BssECI CCNNGG 2 cut(s) 652, 757
BssMI GATC 9 cut(s) 436, 472, 490, 550, 751, 822, 936, 1030, 1123
Bst4CI ACNGT 4 cut(s) 200, 332, 761, 795
BstC8I GCNNGC 1 cut(s) 1061
BstDEI CTNAG 5 cut(s) 290, 468, 830, 882, 1120
BstDSI CCRYGG 1 cut(s) 757
BstF5I GGATG 4 cut(s) 417, 633, 720, 1060
BstFNI CGCG 1 cut(s) 451
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 9 cut(s) 439, 475, 493, 553, 754, 825, 939, 1033, 1126
BstMAI GTCTC 2 cut(s) 132, 784
BstMBI GATC 9 cut(s) 436, 472, 490, 550, 751, 822, 936, 1030, 1123
BstMWI GCNNNNNNNGC 6 cut(s) 179, 230, 280, 341, 762, 1056
BstNSI RCATGY 2 cut(s) 45, 1152
BstSCI CCNGG 1 cut(s) 739
BstUI CGCG 1 cut(s) 451
BstV1I GCAGC 5 cut(s) 157, 544, 792, 890, 1063
BstV2I GAAGAC 1 cut(s) 513
BstX2I RGATCY 5 cut(s) 472, 490, 936, 1030, 1123
BstYI RGATCY 5 cut(s) 472, 490, 936, 1030, 1123
BsuI GTATCC 2 cut(s) 666, 737
BsuRI GGCC 4 cut(s) 233, 543, 626, 1159
BtgI CCRYGG 1 cut(s) 757
BtsCI GGATG 4 cut(s) 417, 633, 720, 1060
BtsIMutI CAGTG 4 cut(s) 64, 241, 681, 1114
BveI ACCTGC 1 cut(s) 1007
Cac8I GCNNGC 1 cut(s) 1061
CciI TCATGA 1 cut(s) 952
CfoI GCGC 1 cut(s) 451
Cfr10I RCCGGY 2 cut(s) 155, 1129
Cfr13I GGNCC 2 cut(s) 231, 686
Csp6I GTAC 2 cut(s) 453, 579
CspAI ACCGGT 2 cut(s) 155, 1129
CviAII CATG 8 cut(s) 42, 381, 725, 784, 953, 968, 1023, 1149
CviQI GTAC 2 cut(s) 453, 579
DdeI CTNAG 5 cut(s) 290, 468, 830, 882, 1120
DpnI GATC 9 cut(s) 438, 474, 492, 552, 753, 824, 938, 1032, 1125
DpnII GATC 9 cut(s) 436, 472, 490, 550, 751, 822, 936, 1030, 1123
EaeI YGGCCR 1 cut(s) 624
Eco147I AGGCCT 1 cut(s) 1159
Eco24I GRGCYC 1 cut(s) 737
Eco32I GATATC 1 cut(s) 950
Eco47I GGWCC 1 cut(s) 686
Eco57I CTGAAG 2 cut(s) 81, 330
Eco88I CYCGRG 1 cut(s) 730
EcoRI GAATTC 1 cut(s) 145
EcoRV GATATC 1 cut(s) 950
EcoT22I ATGCAT 2 cut(s) 82, 1150
EcoT38I GRGCYC 1 cut(s) 737
FaeI CATG 8 cut(s) 45, 384, 728, 787, 956, 971, 1026, 1152
FatI CATG 8 cut(s) 41, 380, 724, 783, 952, 967, 1022, 1148
FauNDI CATATG 1 cut(s) 1012
FbaI TGATCA 1 cut(s) 436
Fnu4HI GCNGC 6 cut(s) 171, 368, 558, 806, 879, 1077
FokI GGATG 4 cut(s) 404, 620, 707, 1067
FriOI GRGCYC 1 cut(s) 737
Fsp4HI GCNGC 6 cut(s) 171, 368, 558, 806, 879, 1077
FspBI CTAG 3 cut(s) 50, 554, 593
GlaI GCGC 1 cut(s) 450
GluI GCNGC 6 cut(s) 171, 368, 558, 806, 879, 1077
GsaI CCCAGC 2 cut(s) 278, 1054
HaeIII GGCC 4 cut(s) 233, 543, 626, 1159
HapII CCGG 3 cut(s) 156, 741, 1130
HhaI GCGC 1 cut(s) 451
Hin1II CATG 8 cut(s) 45, 384, 728, 787, 956, 971, 1026, 1152
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HincII GTYRAC 1 cut(s) 616
HindII GTYRAC 1 cut(s) 616
HindIII AAGCTT 1 cut(s) 911
HinfI GANTC 4 cut(s) 140, 305, 377, 400
HpaI GTTAAC 1 cut(s) 616
HpaII CCGG 3 cut(s) 156, 741, 1130
HphI GGTGA 3 cut(s) 78, 183, 212
Hpy166II GTNNAC 1 cut(s) 616
Hpy188I TCNGA 8 cut(s) 151, 315, 326, 481, 570, 638, 1101, 1123
Hpy188III TCNNGA 4 cut(s) 348, 605, 670, 953
Hpy8I GTNNAC 1 cut(s) 616
HpyAV CCTTC 4 cut(s) 99, 222, 829, 1044
HpyCH4III ACNGT 4 cut(s) 200, 332, 761, 795
HpyF10VI GCNNNNNNNGC 6 cut(s) 179, 230, 280, 341, 762, 1056
HpyF3I CTNAG 5 cut(s) 290, 468, 830, 882, 1120
Hsp92II CATG 8 cut(s) 45, 384, 728, 787, 956, 971, 1026, 1152
HspAI GCGC 1 cut(s) 449
Ksp22I TGATCA 1 cut(s) 436
KspAI GTTAAC 1 cut(s) 616
Kzo9I GATC 9 cut(s) 436, 472, 490, 550, 751, 822, 936, 1030, 1123
LmnI GCTCC 2 cut(s) 315, 353
Lsp1109I GCAGC 5 cut(s) 157, 544, 792, 890, 1063
LweI GCATC 3 cut(s) 67, 89, 197
MaeI CTAG 3 cut(s) 50, 554, 593
MaeIII GTNAC 3 cut(s) 37, 84, 706
MalI GATC 9 cut(s) 438, 474, 492, 552, 753, 824, 938, 1032, 1125
MboI GATC 9 cut(s) 436, 472, 490, 550, 751, 822, 936, 1030, 1123
MboII GAAGA 5 cut(s) 86, 202, 518, 968, 1040
MflI RGATCY 5 cut(s) 472, 490, 936, 1030, 1123
MhlI GDGCHC 1 cut(s) 737
MlsI TGGCCA 1 cut(s) 626
MluCI AATT 6 cut(s) 145, 309, 319, 337, 587, 600
MluNI TGGCCA 1 cut(s) 626
MlyI GAGTC 4 cut(s) 134, 299, 386, 409
MmeI TCCRAC 1 cut(s) 850
MnlI CCTC 6 cut(s) 59, 344, 647, 747, 855, 1066
Mox20I TGGCCA 1 cut(s) 626
Mph1103I ATGCAT 2 cut(s) 82, 1150
MscI TGGCCA 1 cut(s) 626
MseI TTAA 2 cut(s) 615, 1162
MslI CAYNNNNRTG 1 cut(s) 1068
Msp20I TGGCCA 1 cut(s) 626
MspA1I CMGCKG 2 cut(s) 170, 224
MspI CCGG 3 cut(s) 156, 741, 1130
MspR9I CCNGG 1 cut(s) 741
Mva1269I GAATGC 2 cut(s) 173, 665
MvnI CGCG 1 cut(s) 451
MwoI GCNNNNNNNGC 6 cut(s) 179, 230, 280, 341, 762, 1056
NciI CCSGG 1 cut(s) 741
NdeI CATATG 1 cut(s) 1012
NdeII GATC 9 cut(s) 436, 472, 490, 550, 751, 822, 936, 1030, 1123
NlaIII CATG 8 cut(s) 45, 384, 728, 787, 956, 971, 1026, 1152
NlaIV GGNNCC 1 cut(s) 334
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 2 cut(s) 84, 706
NsiI ATGCAT 2 cut(s) 82, 1150
NspI RCATGY 2 cut(s) 45, 1152
PagI TCATGA 1 cut(s) 952
PceI AGGCCT 1 cut(s) 1159
PciI ACATGT 1 cut(s) 41
PctI GAATGC 2 cut(s) 173, 665
PfoI TCCNGGA 1 cut(s) 739
PinAI ACCGGT 2 cut(s) 155, 1129
PkrI GCNGC 6 cut(s) 172, 369, 559, 807, 880, 1078
PleI GAGTC 4 cut(s) 134, 299, 385, 408
PpsI GAGTC 4 cut(s) 134, 299, 385, 408
PscI ACATGT 1 cut(s) 41
PspFI CCCAGC 2 cut(s) 274, 1050
PspN4I GGNNCC 1 cut(s) 334
PspPI GGNCC 2 cut(s) 231, 686
PsrI GAACNNNNNNTAC 2 cut(s) 425, 457
PsuI RGATCY 5 cut(s) 472, 490, 936, 1030, 1123
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 2 cut(s) 454, 580
RsaNI GTAC 2 cut(s) 453, 579
RseI CAYNNNNRTG 1 cut(s) 1068
SaqAI TTAA 2 cut(s) 615, 1162
SatI GCNGC 6 cut(s) 171, 368, 558, 806, 879, 1077
Sau3AI GATC 9 cut(s) 436, 472, 490, 550, 751, 822, 936, 1030, 1123
Sau96I GGNCC 2 cut(s) 231, 686
SchI GAGTC 4 cut(s) 134, 299, 386, 409
ScrFI CCNGG 1 cut(s) 741
SduI GDGCHC 1 cut(s) 737
SfaNI GCATC 3 cut(s) 67, 89, 197
SinI GGWCC 1 cut(s) 686
SmiMI CAYNNNNRTG 1 cut(s) 1068
Sse9I AATT 6 cut(s) 145, 309, 319, 337, 587, 600
SseBI AGGCCT 1 cut(s) 1159
SsiI CCGC 2 cut(s) 168, 367
SspMI CTAG 3 cut(s) 50, 554, 593
StuI AGGCCT 1 cut(s) 1159
StyD4I CCNGG 1 cut(s) 739
TaaI ACNGT 4 cut(s) 200, 332, 761, 795
TaqI TCGA 2 cut(s) 143, 924
TaqII GACCGA 1 cut(s) 666
TasI AATT 6 cut(s) 145, 309, 319, 337, 587, 600
TauI GCSGC 1 cut(s) 370
Tru1I TTAA 2 cut(s) 615, 1162
Tru9I TTAA 2 cut(s) 615, 1162
TscAI CASTG 4 cut(s) 64, 241, 688, 1114
TseFI GTSAC 2 cut(s) 84, 706
TseI GCWGC 5 cut(s) 170, 557, 805, 878, 1076
Tsp45I GTSAC 2 cut(s) 84, 706
TspDTI ATGAA 4 cut(s) 285, 969, 1011, 1104
TspRI CASTG 4 cut(s) 64, 241, 688, 1114
VpaK11BI GGWCC 1 cut(s) 686
XapI RAATTY 2 cut(s) 145, 600
XceI RCATGY 2 cut(s) 45, 1152
XcmI CCANNNNNNNNNTGG 2 cut(s) 221, 974
XspI CTAG 3 cut(s) 50, 554, 593
Zsp2I ATGCAT 2 cut(s) 82, 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.