Rh2AG648300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002827
Physical Location & Seq
Reverse (-)
86812690 .. 86814256
1567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG648300.1

Sequence Viewer

Length: 1161 bp
ATGGTGGGAATGGAGATTTGGAAACAACTACGGCTACTGTCTTCTGGGTTGTTCTTGGCTTTGTGCGTTCTGGGTGTGAGCGGTGGTGCGTTACCATCTTGTTCTTTTCCGGCAATCTACAACTTCGGCGACTCGAACTCCGACACCGGCGGGATCTCAGCCGCATTTGAACCGATTCGGGCACCCTACGGCGAGGGGTTCTTTCGTAAACCAGCTGGAAGGGACTCCGATGGTCGTCTCATCATAGACTTCATAGCTGAGCACCTAAGGTTGCCATACTTGAGTGCATACTTGAACTCGCTGGGAACAAATTACGGGCATGGTGCAAATTTTGCCACCGGAGGATCAACCATTAGAAGGCCGAATGAGACCATTTTCGAGTATGGCATAAGCCCCTTCTCGCTTGATATGCAGACGGCTCAGTTCCTGCAGTTCAAAGCTCGAACGGCTGATCTATTTCGACAAGCCAAGAATCCCTCTGATAGAAGCAAACTTCCAAATCCTCAAGACTTTGCAAAGGCACTCTATACGTTTGATATTGGGCAAAATGATCTCTCTGTTGGTTTTCGAAAGCTAAGCTTTGATCAGCTTCGTGCAGAAATTCCAGACATTGTAAACCAGCTGGCCACAGCAGTCCGACGTATATATGAACAAGGGGGGAGGGCATTTTGGATACACAACACTGGTCCGATTGGCTGTTTGCCAGTGAATCTGTTCTACAATCTTAATCCCGCACCAGGTTATGTAGACGAACATGGCTGTGTCAAGGGCCAAAACGACATGGCCATAGAATTCAACAGGCAGCTCAAGGACAGAGTCATCAAACTAAGAGCTGAGCTCCCACAAGCTGCAATTACATATGTAGATGCATATGCTGCTAAGTATGGACTCATCAGCAATGCAAAGAATGAAGGGTTTGTGGATCCAATGAAGGTTTGCTGTGGGTATCATGTGCGATATGACCATGTGTGGTGTGGAAACAAAGCAACTGTAAATGGAAGTCTAGTATATGGTGCTTCATGTGGAAATCCTTCATCTTCTATAAGCTGGGATGGAGTTCACTACACTCAGGCTGCCAATCAGTGGGTTGCTAATCATATACTCAATGGCGCCTTGTCCAACCCACCAATTCCCATTACTCAAGCTTGTCATAGGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

386

Amino Acids

42.38

Weight (kDa)

8.3

Isoelectric Point (pI)

33.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 39 - 367 4.5e-44 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 181, 1109
AccB7I CCANNNNNTGG 1 cut(s) 1083
AccBSI CCGCTC 1 cut(s) 81
AccI GTMKAC 1 cut(s) 747
AciI CCGC 4 cut(s) 81, 150, 162, 732
AclWI GGATC 4 cut(s) 161, 352, 917, 930
AcoI YGGCCR 2 cut(s) 624, 783
AcsI RAATTY 3 cut(s) 328, 600, 791
AcyI GRCGYC 1 cut(s) 1110
AfiI CCNNNNNNNGG 3 cut(s) 357, 737, 1083
AgsI TTSAA 4 cut(s) 170, 295, 436, 796
AjnI CCWGG 1 cut(s) 736
AjuI GAANNNNNNNTTGG 1 cut(s) 33
Alw21I GWGCWC 2 cut(s) 264, 840
Alw26I GTCTC 2 cut(s) 242, 362
AlwI GGATC 4 cut(s) 161, 352, 917, 930
AlwNI CAGNNNCTG 1 cut(s) 427
AoxI GGCC 4 cut(s) 359, 624, 769, 783
ApeKI GCWGC 4 cut(s) 802, 848, 875, 1073
ApoI RAATTY 3 cut(s) 328, 600, 791
Asp700I GAANNNNTTC 3 cut(s) 174, 713, 1030
AspLEI GCGC 1 cut(s) 1112
AspS9I GGNCC 2 cut(s) 686, 769
AsuII TTCGAA 1 cut(s) 568
AvaII GGWCC 1 cut(s) 686
AxyI CCTNAGG 1 cut(s) 266
BaeGI GKGCMC 1 cut(s) 184
BalI TGGCCA 2 cut(s) 626, 785
BamHI GGATCC 1 cut(s) 922
BanI GGYRCC 2 cut(s) 181, 1109
BanII GRGCYC 1 cut(s) 840
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 2 cut(s) 264, 840
BbvI GCAGC 4 cut(s) 814, 835, 862, 1060
BccI CCATC 3 cut(s) 103, 224, 1046
BceAI ACGGC 4 cut(s) 47, 205, 432, 462
BciT130I CCWGG 1 cut(s) 738
BciVI GTATCC 1 cut(s) 666
BclI TGATCA 1 cut(s) 583
BcoDI GTCTC 2 cut(s) 242, 362
BfaI CTAG 2 cut(s) 1004, 1159
BfmI CTRYAG 1 cut(s) 428
BfoI RGCGCY 1 cut(s) 1113
BfuI GTATCC 1 cut(s) 666
BisI GCNGC 5 cut(s) 162, 803, 849, 876, 1074
BlpI GCTNAGC 3 cut(s) 258, 575, 834
BlsI GCNGC 5 cut(s) 163, 804, 850, 877, 1075
Bme1390I CCNGG 1 cut(s) 738
Bme18I GGWCC 1 cut(s) 686
BmgT120I GGNCC 2 cut(s) 686, 769
BmiI GGNNCC 3 cut(s) 183, 924, 1111
BmrFI CCNGG 1 cut(s) 738
BmsI GCATC 1 cut(s) 856
BpiI GAAGAC 1 cut(s) 33
BplI GAGNNNNNCTC 2 cut(s) 822, 854
Bpu1102I GCTNAGC 3 cut(s) 258, 575, 834
Bpu14I TTCGAA 1 cut(s) 568
BpuEI CTTGAG 4 cut(s) 301, 489, 791, 1125
BsaHI GRCGYC 1 cut(s) 1110
BsaI GGTCTC 1 cut(s) 362
BsaWI WCCGGW 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 3 cut(s) 357, 737, 1083
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 2 cut(s) 688, 704
Bse21I CCTNAGG 1 cut(s) 266
Bse3DI GCAATG 1 cut(s) 904
BseBI CCWGG 1 cut(s) 738
BseGI GGATG 1 cut(s) 1057
BseLI CCNNNNNNNGG 3 cut(s) 357, 737, 1083
BseMI GCAATG 1 cut(s) 904
BseMII CTCAG 5 cut(s) 171, 249, 434, 825, 1082
BseNI ACTGG 2 cut(s) 688, 704
BseSI GKGCMC 1 cut(s) 184
BseXI GCAGC 4 cut(s) 814, 835, 862, 1060
BseYI CCCAGC 2 cut(s) 301, 1047
BsgI GTGCAG 1 cut(s) 615
BshFI GGCC 4 cut(s) 361, 626, 771, 785
BshNI GGYRCC 2 cut(s) 181, 1109
BsiHKAI GWGCWC 2 cut(s) 264, 840
BsiSI CCGG 3 cut(s) 110, 147, 339
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 3 cut(s) 357, 737, 1083
BsmAI GTCTC 2 cut(s) 242, 362
BsmBI CGTCTC 1 cut(s) 242
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 4 cut(s) 361, 626, 771, 785
Bso31I GGTCTC 1 cut(s) 362
Bsp119I TTCGAA 1 cut(s) 568
Bsp1286I GDGCHC 3 cut(s) 184, 264, 840
Bsp143I GATC 6 cut(s) 153, 344, 451, 550, 583, 922
Bsp1720I GCTNAGC 3 cut(s) 258, 575, 834
BspACI CCGC 4 cut(s) 81, 150, 162, 732
BspANI GGCC 4 cut(s) 361, 626, 771, 785
BspCNI CTCAG 5 cut(s) 170, 250, 433, 826, 1081
BspLI GGNNCC 3 cut(s) 183, 924, 1111
BspMAI CTGCAG 1 cut(s) 432
BspPI GGATC 4 cut(s) 161, 352, 917, 930
BspT104I TTCGAA 1 cut(s) 568
BspT107I GGYRCC 2 cut(s) 181, 1109
BspTNI GGTCTC 1 cut(s) 362
BsrBI CCGCTC 1 cut(s) 81
BsrDI GCAATG 1 cut(s) 904
BsrFI RCCGGY 1 cut(s) 146
BsrI ACTGG 2 cut(s) 688, 704
BssAI RCCGGY 1 cut(s) 146
BssMI GATC 6 cut(s) 153, 344, 451, 550, 583, 922
BssNI GRCGYC 1 cut(s) 1110
Bst2UI CCWGG 1 cut(s) 738
Bst4CI ACNGT 2 cut(s) 39, 991
BstACI GRCGYC 1 cut(s) 1110
BstAPI GCANNNNNTGC 2 cut(s) 332, 875
BstBI TTCGAA 1 cut(s) 568
BstC8I GCNNGC 1 cut(s) 624
BstDEI CTNAG 9 cut(s) 157, 258, 266, 420, 575, 827, 834, 879, 1068
BstF5I GGATG 1 cut(s) 1057
BstH2I RGCGCY 1 cut(s) 1113
BstHHI GCGC 1 cut(s) 1112
BstKTI GATC 6 cut(s) 156, 347, 454, 553, 586, 925
BstMAI GTCTC 2 cut(s) 242, 362
BstMBI GATC 6 cut(s) 153, 344, 451, 550, 583, 922
BstMWI GCNNNNNNNGC 4 cut(s) 332, 409, 446, 875
BstNI CCWGG 1 cut(s) 738
BstSCI CCNGG 1 cut(s) 736
BstSFI CTRYAG 1 cut(s) 428
BstSLI GKGCMC 1 cut(s) 184
BstV1I GCAGC 4 cut(s) 814, 835, 862, 1060
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 2 cut(s) 153, 922
BstYI RGATCY 2 cut(s) 153, 922
Bsu36I CCTNAGG 1 cut(s) 266
BsuI GTATCC 1 cut(s) 666
BsuRI GGCC 4 cut(s) 361, 626, 771, 785
BtsCI GGATG 1 cut(s) 1057
BtsIMutI CAGTG 3 cut(s) 681, 711, 1088
Cac8I GCNNGC 1 cut(s) 624
CaiI CAGNNNCTG 1 cut(s) 427
CfoI GCGC 1 cut(s) 1112
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 2 cut(s) 686, 769
CsiI ACCWGGT 1 cut(s) 736
CviAII CATG 6 cut(s) 320, 755, 781, 950, 965, 1020
DdeI CTNAG 9 cut(s) 157, 258, 266, 420, 575, 827, 834, 879, 1068
DinI GGCGCC 1 cut(s) 1111
DpnI GATC 6 cut(s) 155, 346, 453, 552, 585, 924
DpnII GATC 6 cut(s) 153, 344, 451, 550, 583, 922
EaeI YGGCCR 2 cut(s) 624, 783
Ecl136II GAGCTC 1 cut(s) 838
Eco24I GRGCYC 1 cut(s) 840
Eco31I GGTCTC 1 cut(s) 362
Eco47I GGWCC 1 cut(s) 686
Eco53kI GAGCTC 1 cut(s) 838
Eco81I CCTNAGG 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 838
EcoRI GAATTC 1 cut(s) 791
EcoRII CCWGG 1 cut(s) 736
EcoT22I ATGCAT 1 cut(s) 871
EcoT38I GRGCYC 1 cut(s) 840
EgeI GGCGCC 1 cut(s) 1111
EheI GGCGCC 1 cut(s) 1111
Esp3I CGTCTC 1 cut(s) 242
FaeI CATG 6 cut(s) 323, 758, 784, 953, 968, 1023
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FaqI GGGAC 1 cut(s) 236
FatI CATG 6 cut(s) 319, 754, 780, 949, 964, 1019
FauI CCCGC 2 cut(s) 143, 739
FauNDI CATATG 2 cut(s) 859, 871
FbaI TGATCA 1 cut(s) 583
FblI GTMKAC 1 cut(s) 747
Fnu4HI GCNGC 5 cut(s) 162, 803, 849, 876, 1074
FokI GGATG 1 cut(s) 1064
FriOI GRGCYC 1 cut(s) 840
Fsp4HI GCNGC 5 cut(s) 162, 803, 849, 876, 1074
FspBI CTAG 2 cut(s) 1004, 1159
GlaI GCGC 1 cut(s) 1111
GluI GCNGC 5 cut(s) 162, 803, 849, 876, 1074
GsaI CCCAGC 2 cut(s) 305, 1051
HaeII RGCGCY 1 cut(s) 1113
HaeIII GGCC 4 cut(s) 361, 626, 771, 785
HapII CCGG 3 cut(s) 110, 147, 339
HhaI GCGC 1 cut(s) 1112
Hin1I GRCGYC 1 cut(s) 1110
Hin1II CATG 6 cut(s) 323, 758, 784, 953, 968, 1023
Hin6I GCGC 1 cut(s) 1110
HinP1I GCGC 1 cut(s) 1110
HindIII AAGCTT 2 cut(s) 577, 1143
HinfI GANTC 7 cut(s) 131, 175, 224, 472, 709, 816, 888
HpaII CCGG 3 cut(s) 110, 147, 339
Hpy166II GTNNAC 4 cut(s) 209, 616, 748, 1060
Hpy188I TCNGA 5 cut(s) 142, 229, 481, 638, 690
Hpy188III TCNNGA 2 cut(s) 506, 605
Hpy8I GTNNAC 4 cut(s) 209, 616, 748, 1060
Hpy99I CGWCG 1 cut(s) 642
HpyAV CCTTC 6 cut(s) 213, 351, 406, 905, 925, 1041
HpyCH4III ACNGT 2 cut(s) 39, 991
HpyCH4IV ACGT 2 cut(s) 530, 640
HpyCH4V TGCA 9 cut(s) 287, 326, 412, 430, 515, 596, 851, 869, 902
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 409, 446, 875
HpyF3I CTNAG 9 cut(s) 157, 258, 266, 420, 575, 827, 834, 879, 1068
HpySE526I ACGT 2 cut(s) 530, 640
Hsp92I GRCGYC 1 cut(s) 1110
Hsp92II CATG 6 cut(s) 323, 758, 784, 953, 968, 1023
HspAI GCGC 1 cut(s) 1110
KasI GGCGCC 1 cut(s) 1109
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 6 cut(s) 153, 344, 451, 550, 583, 922
LmnI GCTCC 2 cut(s) 843, 1155
Lsp1109I GCAGC 4 cut(s) 814, 835, 862, 1060
LweI GCATC 1 cut(s) 856
MabI ACCWGGT 1 cut(s) 736
MaeI CTAG 2 cut(s) 1004, 1159
MaeII ACGT 2 cut(s) 530, 640
MaeIII GTNAC 1 cut(s) 90
MalI GATC 6 cut(s) 155, 346, 453, 552, 585, 924
MbiI CCGCTC 1 cut(s) 81
MboI GATC 6 cut(s) 153, 344, 451, 550, 583, 922
MboII GAAGA 2 cut(s) 33, 1029
MflI RGATCY 2 cut(s) 153, 922
MhlI GDGCHC 3 cut(s) 184, 264, 840
MlsI TGGCCA 2 cut(s) 626, 785
MluCI AATT 6 cut(s) 310, 328, 600, 791, 852, 1128
MluNI TGGCCA 2 cut(s) 626, 785
Mly113I GGCGCC 1 cut(s) 1110
MlyI GAGTC 4 cut(s) 125, 218, 825, 882
MmeI TCCRAC 3 cut(s) 165, 661, 1143
MnlI CCTC 5 cut(s) 187, 335, 487, 513, 654
Mox20I TGGCCA 2 cut(s) 626, 785
Mph1103I ATGCAT 1 cut(s) 871
MroXI GAANNNNTTC 3 cut(s) 174, 713, 1030
MscI TGGCCA 2 cut(s) 626, 785
MseI TTAA 1 cut(s) 726
MslI CAYNNNNRTG 1 cut(s) 759
Msp20I TGGCCA 2 cut(s) 626, 785
MspA1I CMGCKG 2 cut(s) 215, 622
MspI CCGG 3 cut(s) 110, 147, 339
MspR9I CCNGG 1 cut(s) 738
MvaI CCWGG 1 cut(s) 738
MwoI GCNNNNNNNGC 4 cut(s) 332, 409, 446, 875
NarI GGCGCC 1 cut(s) 1110
NdeI CATATG 2 cut(s) 859, 871
NdeII GATC 6 cut(s) 153, 344, 451, 550, 583, 922
NlaIII CATG 6 cut(s) 323, 758, 784, 953, 968, 1023
NlaIV GGNNCC 3 cut(s) 183, 924, 1111
NsiI ATGCAT 1 cut(s) 871
NspV TTCGAA 1 cut(s) 568
PdmI GAANNNNTTC 3 cut(s) 174, 713, 1030
PfeI GAWTC 3 cut(s) 175, 472, 709
PflFI GACNNNGTC 1 cut(s) 815
PflMI CCANNNNNTGG 1 cut(s) 1083
PkrI GCNGC 5 cut(s) 163, 804, 850, 877, 1075
PleI GAGTC 4 cut(s) 125, 218, 824, 882
PluTI GGCGCC 1 cut(s) 1113
PpsI GAGTC 4 cut(s) 125, 218, 824, 882
Psp124BI GAGCTC 1 cut(s) 840
Psp6I CCWGG 1 cut(s) 736
PspFI CCCAGC 2 cut(s) 301, 1047
PspGI CCWGG 1 cut(s) 736
PspN4I GGNNCC 3 cut(s) 183, 924, 1111
PspPI GGNCC 2 cut(s) 686, 769
PstI CTGCAG 1 cut(s) 432
PstNI CAGNNNCTG 1 cut(s) 427
PsuI RGATCY 2 cut(s) 153, 922
PsyI GACNNNGTC 1 cut(s) 815
PvuII CAGCTG 2 cut(s) 215, 622
RseI CAYNNNNRTG 1 cut(s) 759
SacI GAGCTC 1 cut(s) 840
SaqAI TTAA 1 cut(s) 726
SatI GCNGC 5 cut(s) 162, 803, 849, 876, 1074
Sau3AI GATC 6 cut(s) 153, 344, 451, 550, 583, 922
Sau96I GGNCC 2 cut(s) 686, 769
SchI GAGTC 4 cut(s) 125, 218, 825, 882
ScrFI CCNGG 1 cut(s) 738
SduI GDGCHC 3 cut(s) 184, 264, 840
SexAI ACCWGGT 1 cut(s) 736
SfaNI GCATC 1 cut(s) 856
SfcI CTRYAG 1 cut(s) 428
SfoI GGCGCC 1 cut(s) 1111
SfuI TTCGAA 1 cut(s) 568
SgrAI CRCCGGYG 1 cut(s) 146
SinI GGWCC 1 cut(s) 686
SmiMI CAYNNNNRTG 1 cut(s) 759
SmlI CTYRAG 4 cut(s) 280, 504, 806, 1140
SmoI CTYRAG 4 cut(s) 280, 504, 806, 1140
Sse9I AATT 6 cut(s) 310, 328, 600, 791, 852, 1128
SsiI CCGC 4 cut(s) 81, 150, 162, 732
SspDI GGCGCC 1 cut(s) 1109
SspMI CTAG 2 cut(s) 1004, 1159
SstI GAGCTC 1 cut(s) 840
StyD4I CCNGG 1 cut(s) 736
TaaI ACNGT 2 cut(s) 39, 991
TaiI ACGT 2 cut(s) 533, 643
TaqI TCGA 5 cut(s) 134, 378, 442, 460, 568
TasI AATT 6 cut(s) 310, 328, 600, 791, 852, 1128
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 3 cut(s) 175, 472, 709
Tru1I TTAA 1 cut(s) 726
Tru9I TTAA 1 cut(s) 726
TscAI CASTG 3 cut(s) 688, 711, 1088
TseI GCWGC 4 cut(s) 802, 848, 875, 1073
TspDTI ATGAA 6 cut(s) 241, 663, 924, 944, 1008, 1023
TspRI CASTG 3 cut(s) 688, 711, 1088
Tth111I GACNNNGTC 1 cut(s) 815
Van91I CCANNNNNTGG 1 cut(s) 1083
VpaK11BI GGWCC 1 cut(s) 686
XapI RAATTY 3 cut(s) 328, 600, 791
XcmI CCANNNNNNNNNTGG 1 cut(s) 971
XmiI GTMKAC 1 cut(s) 747
XmnI GAANNNNTTC 3 cut(s) 174, 713, 1030
XspI CTAG 2 cut(s) 1004, 1159
Zsp2I ATGCAT 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.