Rw2G053210

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002827
Physical Location & Seq
Reverse (-)
81043246 .. 81045368
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G053210.1

Sequence Viewer

Length: 753 bp
ATGCAGACGGCTCAGTTCCTGCAGTTCAAAGCTCGAACAGCTGATCTATTTCGACAAGCCAAGAATCCCTCTGATAGAAGCAAACTTCCAAATCCTCAAGACTTTGCAAAGGCACTCTATACGTTTGATATTGGGCAAAATGATCTCTCTGTTGGTTTTCGAAAGCTAAGCTTTGATCAGCTTCGTGCAGAAATTCCAGACATTGTAAACCAGCTGGCCACAGCAGTCCGACGTATATATGAACAAGGGGGGAGGGCATTTTGGATACACAACACAGGTCCGATTGGTTGTTTGCCAGTGAATCTGTTCTACAATCTTAATCCCGCACCAGGTTATGTAGACGAACATGGCTGTGTCAAGGGCCAAAATGACATGGCCATAGAATTCAACAGGCAGCTCAAGGACAGAGTCATCAAACTAAGAGCTGAGCTCCCACAAGCTGCAATTACATATATAGATGCCTATGCTGCTAAGTATGGACTCATCAGCAATGCAAAGAATGAAGGGTTTGTGGATCCAATGAAGGTTTGCTGTGGGTATCATGTGCGATATGACCATGTGTGGTGTGGAAACAAAGCAACTGTAAATGGAAGTCTAGTATATGGTGCTTCATGTGGAAATCCTTCATCTTCTATAAGCTGGGATGGAGTTCACTACACTCAGGCTGCCAATCAGTGGGTTGCTAATCATATACTCAATGGCGCCTTGTCCAGCCCATCAATTCCCATTACTCAAGCTTGTCATAGGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

250

Amino Acids

27.76

Weight (kDa)

8.79

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 4 - 231 1.4e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 701
AccB7I CCANNNNNTGG 1 cut(s) 675
AccI GTMKAC 1 cut(s) 339
AciI CCGC 1 cut(s) 324
AclWI GGATC 2 cut(s) 509, 522
AcoI YGGCCR 2 cut(s) 216, 375
AcsI RAATTY 2 cut(s) 192, 383
AcyI GRCGYC 1 cut(s) 702
AfiI CCNNNNNNNGG 2 cut(s) 329, 675
AgsI TTSAA 2 cut(s) 28, 388
AjnI CCWGG 1 cut(s) 328
Alw21I GWGCWC 1 cut(s) 432
AlwI GGATC 2 cut(s) 509, 522
AlwNI CAGNNNCTG 1 cut(s) 19
AoxI GGCC 3 cut(s) 216, 361, 375
ApeKI GCWGC 4 cut(s) 394, 440, 467, 665
ApoI RAATTY 2 cut(s) 192, 383
Asp700I GAANNNNTTC 2 cut(s) 305, 622
AspLEI GCGC 1 cut(s) 704
AspS9I GGNCC 2 cut(s) 278, 361
AsuII TTCGAA 1 cut(s) 160
AvaII GGWCC 1 cut(s) 278
BalI TGGCCA 2 cut(s) 218, 377
BamHI GGATCC 1 cut(s) 514
BanI GGYRCC 1 cut(s) 701
BanII GRGCYC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 432
BbvI GCAGC 4 cut(s) 406, 427, 454, 652
BccI CCATC 2 cut(s) 638, 724
BceAI ACGGC 1 cut(s) 24
BciT130I CCWGG 1 cut(s) 330
BciVI GTATCC 1 cut(s) 258
BclI TGATCA 1 cut(s) 175
BfaI CTAG 2 cut(s) 596, 751
BfmI CTRYAG 1 cut(s) 20
BfoI RGCGCY 1 cut(s) 705
BfuI GTATCC 1 cut(s) 258
BisI GCNGC 4 cut(s) 395, 441, 468, 666
BlpI GCTNAGC 2 cut(s) 167, 426
BlsI GCNGC 4 cut(s) 396, 442, 469, 667
Bme1390I CCNGG 1 cut(s) 330
Bme18I GGWCC 1 cut(s) 278
BmgT120I GGNCC 2 cut(s) 278, 361
BmiI GGNNCC 2 cut(s) 516, 703
BmrFI CCNGG 1 cut(s) 330
BmsI GCATC 1 cut(s) 448
BplI GAGNNNNNCTC 2 cut(s) 414, 446
Bpu1102I GCTNAGC 2 cut(s) 167, 426
Bpu14I TTCGAA 1 cut(s) 160
BpuEI CTTGAG 3 cut(s) 81, 383, 717
BsaHI GRCGYC 1 cut(s) 702
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 675
Bse1I ACTGG 1 cut(s) 296
Bse3DI GCAATG 1 cut(s) 496
BseBI CCWGG 1 cut(s) 330
BseGI GGATG 1 cut(s) 649
BseLI CCNNNNNNNGG 2 cut(s) 329, 675
BseMI GCAATG 1 cut(s) 496
BseMII CTCAG 3 cut(s) 26, 417, 674
BseNI ACTGG 1 cut(s) 296
BseXI GCAGC 4 cut(s) 406, 427, 454, 652
BseYI CCCAGC 1 cut(s) 639
BsgI GTGCAG 1 cut(s) 207
BshFI GGCC 3 cut(s) 218, 363, 377
BshNI GGYRCC 1 cut(s) 701
BsiHKAI GWGCWC 1 cut(s) 432
BslI CCNNNNNNNGG 2 cut(s) 329, 675
BsnI GGCC 3 cut(s) 218, 363, 377
Bsp119I TTCGAA 1 cut(s) 160
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 4 cut(s) 43, 142, 175, 514
Bsp1720I GCTNAGC 2 cut(s) 167, 426
BspACI CCGC 1 cut(s) 324
BspANI GGCC 3 cut(s) 218, 363, 377
BspCNI CTCAG 3 cut(s) 25, 418, 673
BspLI GGNNCC 2 cut(s) 516, 703
BspMAI CTGCAG 1 cut(s) 24
BspPI GGATC 2 cut(s) 509, 522
BspT104I TTCGAA 1 cut(s) 160
BspT107I GGYRCC 1 cut(s) 701
BsrDI GCAATG 1 cut(s) 496
BsrI ACTGG 1 cut(s) 296
BssMI GATC 4 cut(s) 43, 142, 175, 514
BssNI GRCGYC 1 cut(s) 702
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 583
BstACI GRCGYC 1 cut(s) 702
BstBI TTCGAA 1 cut(s) 160
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 6 cut(s) 12, 167, 419, 426, 471, 660
BstF5I GGATG 1 cut(s) 649
BstH2I RGCGCY 1 cut(s) 705
BstHHI GCGC 1 cut(s) 704
BstKTI GATC 4 cut(s) 46, 145, 178, 517
BstMBI GATC 4 cut(s) 43, 142, 175, 514
BstMWI GCNNNNNNNGC 2 cut(s) 38, 467
BstNI CCWGG 1 cut(s) 330
BstSCI CCNGG 1 cut(s) 328
BstSFI CTRYAG 1 cut(s) 20
BstV1I GCAGC 4 cut(s) 406, 427, 454, 652
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
BsuI GTATCC 1 cut(s) 258
BsuRI GGCC 3 cut(s) 218, 363, 377
BtsCI GGATG 1 cut(s) 649
BtsIMutI CAGTG 2 cut(s) 303, 680
Cac8I GCNNGC 1 cut(s) 216
CaiI CAGNNNCTG 1 cut(s) 19
CfoI GCGC 1 cut(s) 704
Cfr13I GGNCC 2 cut(s) 278, 361
CsiI ACCWGGT 1 cut(s) 328
CviAII CATG 5 cut(s) 347, 373, 542, 557, 612
DdeI CTNAG 6 cut(s) 12, 167, 419, 426, 471, 660
DinI GGCGCC 1 cut(s) 703
DpnI GATC 4 cut(s) 45, 144, 177, 516
DpnII GATC 4 cut(s) 43, 142, 175, 514
EaeI YGGCCR 2 cut(s) 216, 375
Ecl136II GAGCTC 1 cut(s) 430
Eco24I GRGCYC 1 cut(s) 432
Eco47I GGWCC 1 cut(s) 278
Eco53kI GAGCTC 1 cut(s) 430
EcoICRI GAGCTC 1 cut(s) 430
EcoRI GAATTC 1 cut(s) 383
EcoRII CCWGG 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 432
EgeI GGCGCC 1 cut(s) 703
EheI GGCGCC 1 cut(s) 703
FaeI CATG 5 cut(s) 350, 376, 545, 560, 615
FalI AAGNNNNNCTT 2 cut(s) 155, 187
FatI CATG 5 cut(s) 346, 372, 541, 556, 611
FauI CCCGC 1 cut(s) 331
FbaI TGATCA 1 cut(s) 175
FblI GTMKAC 1 cut(s) 339
Fnu4HI GCNGC 4 cut(s) 395, 441, 468, 666
FokI GGATG 1 cut(s) 656
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 4 cut(s) 395, 441, 468, 666
FspBI CTAG 2 cut(s) 596, 751
GlaI GCGC 1 cut(s) 703
GluI GCNGC 4 cut(s) 395, 441, 468, 666
GsaI CCCAGC 1 cut(s) 643
HaeII RGCGCY 1 cut(s) 705
HaeIII GGCC 3 cut(s) 218, 363, 377
HhaI GCGC 1 cut(s) 704
Hin1I GRCGYC 1 cut(s) 702
Hin1II CATG 5 cut(s) 350, 376, 545, 560, 615
Hin6I GCGC 1 cut(s) 702
HinP1I GCGC 1 cut(s) 702
HindIII AAGCTT 2 cut(s) 169, 735
HinfI GANTC 4 cut(s) 64, 301, 408, 480
Hpy166II GTNNAC 3 cut(s) 208, 340, 652
Hpy188I TCNGA 3 cut(s) 73, 230, 282
Hpy188III TCNNGA 2 cut(s) 98, 197
Hpy8I GTNNAC 3 cut(s) 208, 340, 652
Hpy99I CGWCG 1 cut(s) 234
HpyAV CCTTC 3 cut(s) 497, 517, 633
HpyCH4III ACNGT 1 cut(s) 583
HpyCH4IV ACGT 2 cut(s) 122, 232
HpyCH4V TGCA 6 cut(s) 4, 22, 107, 188, 443, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 467
HpyF3I CTNAG 6 cut(s) 12, 167, 419, 426, 471, 660
HpySE526I ACGT 2 cut(s) 122, 232
Hsp92I GRCGYC 1 cut(s) 702
Hsp92II CATG 5 cut(s) 350, 376, 545, 560, 615
HspAI GCGC 1 cut(s) 702
KasI GGCGCC 1 cut(s) 701
Ksp22I TGATCA 1 cut(s) 175
Kzo9I GATC 4 cut(s) 43, 142, 175, 514
LmnI GCTCC 2 cut(s) 435, 747
Lsp1109I GCAGC 4 cut(s) 406, 427, 454, 652
LweI GCATC 1 cut(s) 448
MabI ACCWGGT 1 cut(s) 328
MaeI CTAG 2 cut(s) 596, 751
MaeII ACGT 2 cut(s) 122, 232
MalI GATC 4 cut(s) 45, 144, 177, 516
MboI GATC 4 cut(s) 43, 142, 175, 514
MboII GAAGA 1 cut(s) 621
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 1 cut(s) 432
MlsI TGGCCA 2 cut(s) 218, 377
MluCI AATT 4 cut(s) 192, 383, 444, 720
MluNI TGGCCA 2 cut(s) 218, 377
Mly113I GGCGCC 1 cut(s) 702
MlyI GAGTC 2 cut(s) 417, 474
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 3 cut(s) 79, 105, 246
Mox20I TGGCCA 2 cut(s) 218, 377
MroXI GAANNNNTTC 2 cut(s) 305, 622
MscI TGGCCA 2 cut(s) 218, 377
MseI TTAA 1 cut(s) 318
MslI CAYNNNNRTG 1 cut(s) 351
Msp20I TGGCCA 2 cut(s) 218, 377
MspA1I CMGCKG 2 cut(s) 41, 214
MspR9I CCNGG 1 cut(s) 330
MvaI CCWGG 1 cut(s) 330
MwoI GCNNNNNNNGC 2 cut(s) 38, 467
NarI GGCGCC 1 cut(s) 702
NdeII GATC 4 cut(s) 43, 142, 175, 514
NlaIII CATG 5 cut(s) 350, 376, 545, 560, 615
NlaIV GGNNCC 2 cut(s) 516, 703
NspV TTCGAA 1 cut(s) 160
PdmI GAANNNNTTC 2 cut(s) 305, 622
PfeI GAWTC 2 cut(s) 64, 301
PflFI GACNNNGTC 1 cut(s) 407
PflMI CCANNNNNTGG 1 cut(s) 675
PkrI GCNGC 4 cut(s) 396, 442, 469, 667
PleI GAGTC 2 cut(s) 416, 474
PluTI GGCGCC 1 cut(s) 705
PpsI GAGTC 2 cut(s) 416, 474
Psp124BI GAGCTC 1 cut(s) 432
Psp6I CCWGG 1 cut(s) 328
PspFI CCCAGC 1 cut(s) 639
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 2 cut(s) 516, 703
PspPI GGNCC 2 cut(s) 278, 361
PstI CTGCAG 1 cut(s) 24
PstNI CAGNNNCTG 1 cut(s) 19
PsuI RGATCY 1 cut(s) 514
PsyI GACNNNGTC 1 cut(s) 407
PvuII CAGCTG 2 cut(s) 41, 214
RseI CAYNNNNRTG 1 cut(s) 351
SacI GAGCTC 1 cut(s) 432
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 4 cut(s) 395, 441, 468, 666
Sau3AI GATC 4 cut(s) 43, 142, 175, 514
Sau96I GGNCC 2 cut(s) 278, 361
SchI GAGTC 2 cut(s) 417, 474
ScrFI CCNGG 1 cut(s) 330
SduI GDGCHC 1 cut(s) 432
SexAI ACCWGGT 1 cut(s) 328
SfaNI GCATC 1 cut(s) 448
SfcI CTRYAG 1 cut(s) 20
SfoI GGCGCC 1 cut(s) 703
SfuI TTCGAA 1 cut(s) 160
SinI GGWCC 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 351
SmlI CTYRAG 3 cut(s) 96, 398, 732
SmoI CTYRAG 3 cut(s) 96, 398, 732
Sse9I AATT 4 cut(s) 192, 383, 444, 720
SsiI CCGC 1 cut(s) 324
SspDI GGCGCC 1 cut(s) 701
SspMI CTAG 2 cut(s) 596, 751
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 1 cut(s) 328
TaaI ACNGT 1 cut(s) 583
TaiI ACGT 2 cut(s) 125, 235
TaqI TCGA 3 cut(s) 34, 52, 160
TasI AATT 4 cut(s) 192, 383, 444, 720
TfiI GAWTC 2 cut(s) 64, 301
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 2 cut(s) 303, 680
TseI GCWGC 4 cut(s) 394, 440, 467, 665
TspDTI ATGAA 5 cut(s) 255, 516, 536, 600, 615
TspRI CASTG 2 cut(s) 303, 680
Tth111I GACNNNGTC 1 cut(s) 407
Van91I CCANNNNNTGG 1 cut(s) 675
VpaK11BI GGWCC 1 cut(s) 278
XapI RAATTY 2 cut(s) 192, 383
XcmI CCANNNNNNNNNTGG 1 cut(s) 563
XmiI GTMKAC 1 cut(s) 339
XmnI GAANNNNTTC 2 cut(s) 305, 622
XspI CTAG 2 cut(s) 596, 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.