MD17G1021700.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002827
Physical Location & Seq
Forward (+)
1617444 .. 1619693
2250 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1021700.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGGAGTTTTGGAAACTGCTTCTGGCATCCGGGTTCTTCTTGGCTTCGTGGGTTTTGGCAGTGGGAGGCCAGAACTTACCACCCTGTTCTTTTCCGGCGATTTACAACTTCGGGGACTCAAATTCCGACACCGGCGGCATATCCGCCGCATTTGAGCCAATCAAACCTCCTAACGGCGAAGCATTCTTCCATAAGCCGGCCGGGAGGGCCGCCGATGGCCGCCTTGTCATAGACTTCATAGCTGAGCGCCTAAAGCTGCCGTACCTGAGTGCATATTTGAACTCACTTGGAACAAATTACCGGCACGGTGCGAATTTCGCCACCGGCGGATCCACCATCAGAAGACCAAACGAAACCATCTTTGAGAACGGCATTAGTCCTTTCTCCCTCGATATGCAGACGGCGCAATTCCTGCAGTTCAAGGCCCGCACCGCCGATCTCTACCGCCAAGCCAAGAACCCTTCTCAGAGAAGCGCTCTGCCGAATCCTCAAGATTTCGCAAAGGCTCTCTACACGTTTGATATTGGGCAAAATGATCTATCCTCCGGCTTTCGGAAGCTAAGTTTCGATCAGCTTCGCGCACAACTGCCGGACATTGTCAACCAGTTAGCCACAGCAGTCCATCGGATATATGAAGAAGGAGGGAGGACATTTTGGATACACAACACAGGTCCTGTTGGTTGCTTGCCTATACAGTTATTCTACAATCTGAATCCGCCACCCGGTTTTCTCGACGACCACGGCTGCGTCAACGCCCAAAACAACATCGCCGTAGAGTTCAACAGGCAGCTAAGGGACAGAGTCACCAAACTCAGAGCCGAGCTCCCACAAGCTGCGATCACATATGTCGATGTCTTTGCCGCCAAGTATGGACTCATCGGCACTGCAAAGACCGAAGGATTCGCCGAACCAATGAAGGTTTGCTGCGGGTATCATGTGAAATACGACCATGTCTGGTGCGGGAATAAAGGAAGTGCGAGCGGAAGGGAAGTGTACGGTGCTTCATGTCAAAACCCGTCGGCTTTTATCAGCTGGGATGGAGTTCACTACACTCAGGGTGCGAACCAGTGGGTTGCTAATCGTATACTCAATGGCTCACCGTCGTCAAACCCGGCAATTCCGATTACTCAAGCTTGTCATAGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

383

Amino Acids

42.06

Weight (kDa)

8.55

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 362 3.1e-43 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 981
AccI GTMKAC 1 cut(s) 1084
AccII CGCG 1 cut(s) 579
AclWI GGATC 2 cut(s) 324, 337
AcoI YGGCCR 2 cut(s) 198, 217
AcsI RAATTY 2 cut(s) 121, 313
AfaI GTAC 2 cut(s) 263, 995
AfeI AGCGCT 1 cut(s) 475
AfiI CCNNNNNNNGG 4 cut(s) 173, 196, 552, 722
AflIII ACRYGT 1 cut(s) 513
AgsI TTSAA 3 cut(s) 280, 421, 781
AluBI AGCT 9 cut(s) 242, 256, 559, 574, 790, 823, 833, 1032, 1133
AluI AGCT 9 cut(s) 242, 256, 559, 574, 790, 823, 833, 1032, 1133
Alw21I GWGCWC 1 cut(s) 825
AlwI GGATC 2 cut(s) 324, 337
AlwNI CAGNNNCTG 1 cut(s) 674
Aor51HI AGCGCT 1 cut(s) 475
AoxI GGCC 5 cut(s) 67, 198, 207, 217, 423
ApeKI GCWGC 5 cut(s) 256, 744, 787, 833, 924
ApoI RAATTY 2 cut(s) 121, 313
AspLEI GCGC 4 cut(s) 249, 406, 476, 581
AspS9I GGNCC 3 cut(s) 207, 424, 671
AsuC2I CCSGG 4 cut(s) 31, 202, 723, 1112
AsuHPI GGTGA 2 cut(s) 796, 1089
AvaII GGWCC 1 cut(s) 671
BamHI GGATCC 1 cut(s) 329
BanII GRGCYC 1 cut(s) 825
BbsI GAAGAC 1 cut(s) 349
Bbv12I GWGCWC 1 cut(s) 825
BbvI GCAGC 5 cut(s) 243, 731, 799, 820, 911
BccI CCATC 5 cut(s) 209, 344, 365, 630, 1031
BceAI ACGGC 6 cut(s) 190, 244, 385, 417, 755, 757
BcgI CGANNNNNNTGC 2 cut(s) 839, 873
BciVI GTATCC 1 cut(s) 651
BcnI CCSGG 4 cut(s) 31, 202, 723, 1112
BfmI CTRYAG 1 cut(s) 413
BfoI RGCGCY 2 cut(s) 250, 477
BfuI GTATCC 1 cut(s) 651
BglI GCCNNNNNGGC 1 cut(s) 206
BlpI GCTNAGC 1 cut(s) 243
Bme1390I CCNGG 4 cut(s) 31, 202, 723, 1112
Bme18I GGWCC 1 cut(s) 671
BmgT120I GGNCC 3 cut(s) 207, 424, 671
BmiI GGNNCC 1 cut(s) 331
BmrFI CCNGG 4 cut(s) 31, 202, 723, 1112
BmsI GCATC 1 cut(s) 35
BpiI GAAGAC 1 cut(s) 349
BplI GAGNNNNNCTC 4 cut(s) 460, 492, 807, 839
Bpu10I CCTNAGC 1 cut(s) 791
Bpu1102I GCTNAGC 1 cut(s) 243
BpuEI CTTGAG 2 cut(s) 474, 1113
BpuMI CCSGG 4 cut(s) 31, 202, 723, 1112
BsaJI CCNNGG 1 cut(s) 739
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 196, 552, 722
Bse118I RCCGGY 4 cut(s) 131, 196, 300, 323
Bse1I ACTGG 2 cut(s) 604, 1066
BseDI CCNNGG 1 cut(s) 739
BseGI GGATG 2 cut(s) 26, 1042
BseLI CCNNNNNNNGG 4 cut(s) 173, 196, 552, 722
BseMII CTCAG 5 cut(s) 234, 257, 479, 826, 1067
BseNI ACTGG 2 cut(s) 604, 1066
BseX3I CGGCCG 1 cut(s) 198
BseXI GCAGC 5 cut(s) 243, 731, 799, 820, 911
BseYI CCCAGC 1 cut(s) 1032
Bsh1236I CGCG 1 cut(s) 579
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 5 cut(s) 69, 200, 209, 219, 425
BsiEI CGRYCG 1 cut(s) 201
BsiHKAI GWGCWC 1 cut(s) 825
BslFI GGGAC 2 cut(s) 128, 809
BslI CCNNNNNNNGG 4 cut(s) 173, 196, 552, 722
BsmFI GGGAC 2 cut(s) 128, 809
BsmI GAATGC 1 cut(s) 182
BsnI GGCC 5 cut(s) 69, 200, 209, 219, 425
Bsp1286I GDGCHC 1 cut(s) 825
Bsp143I GATC 5 cut(s) 329, 436, 535, 568, 837
Bsp1720I GCTNAGC 1 cut(s) 243
BspANI GGCC 5 cut(s) 69, 200, 209, 219, 425
BspCNI CTCAG 5 cut(s) 235, 258, 478, 825, 1066
BspFNI CGCG 1 cut(s) 579
BspLI GGNNCC 1 cut(s) 331
BspMAI CTGCAG 1 cut(s) 417
BspPI GGATC 2 cut(s) 324, 337
BsrBI CCGCTC 1 cut(s) 981
BsrFI RCCGGY 4 cut(s) 131, 196, 300, 323
BsrI ACTGG 2 cut(s) 604, 1066
BssAI RCCGGY 4 cut(s) 131, 196, 300, 323
BssECI CCNNGG 1 cut(s) 739
BssMI GATC 5 cut(s) 329, 436, 535, 568, 837
BssNAI GTATAC 1 cut(s) 1085
Bst1107I GTATAC 1 cut(s) 1085
Bst4CI ACNGT 4 cut(s) 308, 696, 998, 1101
BstAPI GCANNNNNTGC 1 cut(s) 412
BstC8I GCNNGC 4 cut(s) 198, 427, 686, 979
BstDEI CTNAG 7 cut(s) 243, 266, 465, 560, 791, 812, 1053
BstDSI CCRYGG 1 cut(s) 739
BstF5I GGATG 2 cut(s) 26, 1042
BstFNI CGCG 1 cut(s) 579
BstH2I RGCGCY 2 cut(s) 250, 477
BstHHI GCGC 4 cut(s) 249, 406, 476, 581
BstKTI GATC 5 cut(s) 332, 439, 538, 571, 840
BstMBI GATC 5 cut(s) 329, 436, 535, 568, 837
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 6 cut(s) 206, 253, 317, 403, 412, 431
BstSCI CCNGG 4 cut(s) 29, 200, 721, 1110
BstSFI CTRYAG 1 cut(s) 413
BstUI CGCG 1 cut(s) 579
BstV1I GCAGC 5 cut(s) 243, 731, 799, 820, 911
BstV2I GAAGAC 1 cut(s) 349
BstX2I RGATCY 1 cut(s) 329
BstYI RGATCY 1 cut(s) 329
BstZ17I GTATAC 1 cut(s) 1085
BstZI CGGCCG 1 cut(s) 198
BsuI GTATCC 1 cut(s) 651
BsuRI GGCC 5 cut(s) 69, 200, 209, 219, 425
BtgI CCRYGG 1 cut(s) 739
BtgZI GCGATG 1 cut(s) 751
BtsCI GGATG 2 cut(s) 26, 1042
BtsI GCAGTG 2 cut(s) 66, 882
BtsIMutI CAGTG 3 cut(s) 66, 882, 1073
Cac8I GCNNGC 4 cut(s) 198, 427, 686, 979
CaiI CAGNNNCTG 1 cut(s) 674
CfoI GCGC 4 cut(s) 249, 406, 476, 581
Cfr10I RCCGGY 4 cut(s) 131, 196, 300, 323
Cfr13I GGNCC 3 cut(s) 207, 424, 671
CseI GACGC 1 cut(s) 736
Csp6I GTAC 2 cut(s) 262, 994
CviAII CATG 3 cut(s) 935, 950, 1005
CviQI GTAC 2 cut(s) 262, 994
DdeI CTNAG 7 cut(s) 243, 266, 465, 560, 791, 812, 1053
DpnI GATC 5 cut(s) 331, 438, 537, 570, 839
DpnII GATC 5 cut(s) 329, 436, 535, 568, 837
EaeI YGGCCR 2 cut(s) 198, 217
EagI CGGCCG 1 cut(s) 198
EciI GGCGGA 3 cut(s) 133, 342, 705
Ecl136II GAGCTC 1 cut(s) 823
EclXI CGGCCG 1 cut(s) 198
Eco24I GRGCYC 1 cut(s) 825
Eco47I GGWCC 1 cut(s) 671
Eco47III AGCGCT 1 cut(s) 475
Eco52I CGGCCG 1 cut(s) 198
Eco53kI GAGCTC 1 cut(s) 823
EcoICRI GAGCTC 1 cut(s) 823
EcoO109I RGGNCCY 1 cut(s) 671
EcoT38I GRGCYC 1 cut(s) 825
FaeI CATG 3 cut(s) 938, 953, 1008
FaqI GGGAC 2 cut(s) 128, 809
FatI CATG 3 cut(s) 934, 949, 1004
FauI CCCGC 3 cut(s) 434, 920, 953
FauNDI CATATG 1 cut(s) 844
FblI GTMKAC 1 cut(s) 1084
FokI GGATG 2 cut(s) 13, 1049
FriOI GRGCYC 1 cut(s) 825
GlaI GCGC 4 cut(s) 248, 405, 475, 580
GsaI CCCAGC 1 cut(s) 1036
HaeII RGCGCY 2 cut(s) 250, 477
HaeIII GGCC 5 cut(s) 69, 200, 209, 219, 425
HgaI GACGC 1 cut(s) 736
HhaI GCGC 4 cut(s) 249, 406, 476, 581
Hin1II CATG 3 cut(s) 938, 953, 1008
Hin6I GCGC 4 cut(s) 247, 404, 474, 579
HinP1I GCGC 4 cut(s) 247, 404, 474, 579
HincII GTYRAC 2 cut(s) 601, 751
HindII GTYRAC 2 cut(s) 601, 751
HindIII AAGCTT 1 cut(s) 1131
HinfI GANTC 6 cut(s) 116, 484, 712, 801, 873, 900
HphI GGTGA 2 cut(s) 796, 1089
Hpy166II GTNNAC 5 cut(s) 601, 751, 994, 1045, 1085
Hpy188I TCNGA 8 cut(s) 127, 341, 468, 555, 627, 711, 815, 1122
Hpy188III TCNNGA 2 cut(s) 491, 731
Hpy8I GTNNAC 5 cut(s) 601, 751, 994, 1045, 1085
Hpy99I CGWCG 3 cut(s) 737, 1021, 1105
HpyAV CCTTC 5 cut(s) 471, 632, 890, 910, 978
HpyCH4III ACNGT 4 cut(s) 308, 696, 998, 1101
HpyCH4IV ACGT 1 cut(s) 515
HpyCH4V TGCA 4 cut(s) 272, 397, 415, 887
HpyF10VI GCNNNNNNNGC 6 cut(s) 206, 253, 317, 403, 412, 431
HpyF3I CTNAG 7 cut(s) 243, 266, 465, 560, 791, 812, 1053
HpySE526I ACGT 1 cut(s) 515
Hsp92II CATG 3 cut(s) 938, 953, 1008
HspAI GCGC 4 cut(s) 247, 404, 474, 579
KroI GCCGGC 1 cut(s) 196
KroNI GCCGGC 1 cut(s) 198
Kzo9I GATC 5 cut(s) 329, 436, 535, 568, 837
LmnI GCTCC 1 cut(s) 828
Lsp1109I GCAGC 5 cut(s) 243, 731, 799, 820, 911
LweI GCATC 1 cut(s) 35
MaeII ACGT 1 cut(s) 515
MaeIII GTNAC 1 cut(s) 802
MalI GATC 5 cut(s) 331, 438, 537, 570, 839
MbiI CCGCTC 1 cut(s) 981
MboI GATC 5 cut(s) 329, 436, 535, 568, 837
MboII GAAGA 4 cut(s) 28, 178, 354, 647
MflI RGATCY 1 cut(s) 329
MhlI GDGCHC 1 cut(s) 825
MluCI AATT 5 cut(s) 121, 295, 313, 407, 1116
MlyI GAGTC 3 cut(s) 110, 810, 867
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 8 cut(s) 59, 177, 198, 398, 498, 553, 635, 639
MroNI GCCGGC 1 cut(s) 196
MspA1I CMGCKG 1 cut(s) 1032
MspR9I CCNGG 4 cut(s) 31, 202, 723, 1112
Mva1269I GAATGC 1 cut(s) 182
MvnI CGCG 1 cut(s) 579
MwoI GCNNNNNNNGC 6 cut(s) 206, 253, 317, 403, 412, 431
NaeI GCCGGC 1 cut(s) 198
NciI CCSGG 4 cut(s) 31, 202, 723, 1112
NdeI CATATG 1 cut(s) 844
NdeII GATC 5 cut(s) 329, 436, 535, 568, 837
NgoMIV GCCGGC 1 cut(s) 196
NlaIII CATG 3 cut(s) 938, 953, 1008
NlaIV GGNNCC 1 cut(s) 331
NmeAIII GCCGAG 1 cut(s) 844
NmuCI GTSAC 1 cut(s) 802
PctI GAATGC 1 cut(s) 182
PdiI GCCGGC 1 cut(s) 198
PfeI GAWTC 3 cut(s) 484, 712, 900
PflFI GACNNNGTC 3 cut(s) 596, 800, 950
PleI GAGTC 3 cut(s) 110, 809, 867
PpsI GAGTC 3 cut(s) 110, 809, 867
PpuMI RGGWCCY 1 cut(s) 671
Psp124BI GAGCTC 1 cut(s) 825
Psp5II RGGWCCY 1 cut(s) 671
PspFI CCCAGC 1 cut(s) 1032
PspN4I GGNNCC 1 cut(s) 331
PspPI GGNCC 3 cut(s) 207, 424, 671
PspPPI RGGWCCY 1 cut(s) 671
PstI CTGCAG 1 cut(s) 417
PstNI CAGNNNCTG 1 cut(s) 674
PsuI RGATCY 1 cut(s) 329
PsyI GACNNNGTC 3 cut(s) 596, 800, 950
PvuII CAGCTG 1 cut(s) 1032
RsaI GTAC 2 cut(s) 263, 995
RsaNI GTAC 2 cut(s) 262, 994
SacI GAGCTC 1 cut(s) 825
Sau3AI GATC 5 cut(s) 329, 436, 535, 568, 837
Sau96I GGNCC 3 cut(s) 207, 424, 671
SchI GAGTC 3 cut(s) 110, 810, 867
ScrFI CCNGG 4 cut(s) 31, 202, 723, 1112
SduI GDGCHC 1 cut(s) 825
SfaNI GCATC 1 cut(s) 35
SfcI CTRYAG 1 cut(s) 413
SfiI GGCCNNNNNGGCC 1 cut(s) 206
SgrAI CRCCGGYG 2 cut(s) 131, 323
SinI GGWCC 1 cut(s) 671
SmlI CTYRAG 2 cut(s) 489, 1128
SmoI CTYRAG 2 cut(s) 489, 1128
Sse9I AATT 5 cut(s) 121, 295, 313, 407, 1116
SstI GAGCTC 1 cut(s) 825
StyD4I CCNGG 4 cut(s) 29, 200, 721, 1110
TaaI ACNGT 4 cut(s) 308, 696, 998, 1101
TaiI ACGT 1 cut(s) 518
TaqI TCGA 4 cut(s) 390, 567, 732, 849
TaqII GACCGA 1 cut(s) 908
TasI AATT 5 cut(s) 121, 295, 313, 407, 1116
TauI GCSGC 5 cut(s) 138, 149, 212, 222, 863
TfiI GAWTC 3 cut(s) 484, 712, 900
TscAI CASTG 3 cut(s) 66, 889, 1073
TseFI GTSAC 1 cut(s) 802
TseI GCWGC 5 cut(s) 256, 744, 787, 833, 924
Tsp45I GTSAC 1 cut(s) 802
TspDTI ATGAA 4 cut(s) 226, 648, 929, 993
TspRI CASTG 3 cut(s) 66, 889, 1073
Tth111I GACNNNGTC 3 cut(s) 596, 800, 950
VpaK11BI GGWCC 1 cut(s) 671
XapI RAATTY 2 cut(s) 121, 313
XmiI GTMKAC 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.