Rorug02G0567700

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002827
Physical Location & Seq
Forward (+)
69289639 .. 69292376
2738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0567700.1

Sequence Viewer

Length: 2658 bp
ATGAAACCAATCATCACTCCTCTCTACATCTACTTGTTCCTCCACATCCTCACTCTCTTCGTCACCGGTCAGTCGCCACCATACACTCCGGTCGACAATATCGTCCTCGCCTGTGGCAATTCCGGCCAACCAACCTCCAATGACAACAGAAAGTGGAGTGGAGATATCGACTCAAAATTCACCCCAATTGGTAGCGCATCCGAATCCATAAATGCACCGCCGTCGTCCACCGCTAGCCAAGTCCCTTACACCAAAGCTCGGCTCTCTCGCTCCAAATTCACTTACACAATTCCCCTCTCTCCCGGCCAAAAATTCATCCGCTTACATTTCAATCCAGATACATACACCCCGGACTTCCAACGCCCCAACTCCCTCTTCTCCGTCACAGCCGCCGGCTTCACTCTCCTCAAAGACTTCAACGCCTCCGTCACCGCCGACGCTTCCGGCGAGTCCCCGCTGGTCAGAGAATTCTGTGTAAACATCGAGACAGGACAGAGACTCGAGATCACCTTCACACCAAGCAAAGATGCGTACGCTTTCATCAACGGAATCGAAATCGTGTCCATGCCCACCGGTCTTTACTACACCGCAGCCGAAAGCGATTCAGCTAAATTGGTCGACGGCACCGCAAGCTCATACTCGATCGAAAACAGCACAGCTCTGGAGATGGTGTACCGAATCAACGTGGGAGGACGCCCCATCTCGCCGAACGAAGACACCGGAATGTACCGGACTTGGGAAAATGGCGACGATATCTATTTGGATGATATAAGTAGAAAATTGAGCGTACTACCACAGAACAATACAATCGAGCTCAATTTCTCCAAAGTTCCAGAATACTCTGCCCCAAAAGCAGTTTACATAACCGGTCGGTCAATGGGGATGAACAAAACCATCAACAAGAGCTATAACCTCACATGGGTATTTCCCTTAGATCCCAAGTTTAATTACCTGGTGAGACTCCATTTCTGTGAGTTTGAATCTGATATTACCAAGTCCAGAGATCGAGCGTTTCAAATCTTCATGGCCAATCAAACCGCAGAAAAGATTTTTGATGTTATCCAATGGAGTGGAGCAAATGGGAATCCAGTTTACGAAGACTACGTTGTGGGCATGTTCATGTCTCCGGTTGGAAGAGAGAAGAAAGTTAACCTCTTTCTAGTACTCGCAGCGCTAGAGAGAGATTTGTATACTACTTACAACGATGCCATCTTGAACGGGCTAGAAATCTTCAAGCTCAGCGACTCGACTCGGAATCTTGCCGGACCCAACCCCGACCCACCTCCGGTGAAAGCCCCAAAGGTACCACCGAAGAGCACCAGTAAGAAGAAGTCTACTCCTATGGCAGCTATCATTGCAGGCGTAGTTTCCGGGTTACTTATGCTCTCTGTTCTCGGATTTTTTGTTTTCCGGCGAGGAAGAAAGGCCAAGGATTATAGCTCAAGTCAAGTAAAGTGGGGTCTGCTCTCTTTTTCAACGACTACTAAGTCCTCCAAGTCCACCACGTCTTACGGTTCATCTCTGCCGTCCGAGCTGTGTCATCACTTTTCATTGGCGGAGATCAGAGCCGCCACGCAAAACTTTGATGAATCGAACGTTATTGGGCGTGGCGGGTTTGGTAACGTGTACAAAGGTACCATCAACGGTGCGCCCACCCCCTTTGCGATCAAACGGCTGAAAGCGGAGTCGTCACAGGGTGCCCTTGAGTTCAAGACGGAGATCGAATTGCTCTCCCAACTCCGTCACGTCCATCTCGTGTCTCTGGTCGGGTACTGTGACGACAACGGTGAGATGATTTTGGTGTATGACTATATGGAAAATGGGACCCTACGCGATCATTTGTACAACTCCGAAAATCCACCTCTTCCTTGGGAACAGAGGCTTCAAGTTTGTATGGGTGCGGCGCGTGGGTTGCACTACCTTCACACTGGTGTCAAGTGTATGATCATTCACCGAGACGTGAAGAGCACGAACATCTTGTTGGACGATAAATGGGTGGCCAAGGTCGCTGATTTCGGGTTGTCAAAAATGGGCACGACCAACATGTCTAAGACCCACATTAGCACCATGGTCAAGGGCAGTTTCGGCTATCTCGACCCGGAGTACTACCGTCGTCAACAGTTGACTGAGAAGTCTGATGTGTACTCATTCGGCGTAGTATTGTGTGAGGTGTTGTGTGCTAGGCCGGCTTTGATCCGTACAGCGGACAAGAAGCAAATGAACCTAGCTGCATGGTTCAAGACCTGTCATGAAAACGGAACGCTTGACCAAATCATTGACCCGAATTTGAGGGGTAAGATTGTAAATGAGTGCTTGAACAAGTTTGTTGAAATTGCAATTAGTTGTATGCATGATAATGGGAGTGAACGACCGTCCATGAATGATGTTATATGGGCGCTTGATTGTGCATTAAAACTTCAACAGACCGCAGAGGGTTCGGATTTGGATTTCAATGCCGGGAAGAAGGGTGAAGATGAGGCTTCAGTGCTGAATGATAGTGATGTAGGGTTTAGTGGCACATGGGAAGACTCGTCAGGTTCCAAGAACAGCAGGGTGACCAAAACTAGTAGTAGTTACCAAAATTCTACTGCTAGTGATTCCATTAAAGGGCTGTCAGGTACAGTCTTCTCTGAGATCTACGATCCTAATGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

885

Amino Acids

97.93

Weight (kDa)

6.57

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 37 - 412 1.8e-32 Malectin-like domain
Malectin PF11721 280 - 368 7.8e-06 Malectin domain
Pkinase PF00069 530 - 796 1.1e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 532 - 797 1.2e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 4 cut(s) 101, 1486, 2044, 2131
Acc65I GGTACC 2 cut(s) 1303, 1634
AccB1I GGYRCC 4 cut(s) 623, 1303, 1634, 1697
AccI GTMKAC 4 cut(s) 93, 618, 1190, 1334
AccII CGCG 2 cut(s) 1833, 1906
AclI AACGTT 1 cut(s) 1596
AclWI GGATC 3 cut(s) 929, 2188, 2636
AcoI YGGCCR 4 cut(s) 124, 304, 1026, 1998
AcsI RAATTY 6 cut(s) 176, 275, 311, 467, 2284, 2581
AcuI CTGAAG 1 cut(s) 2466
AcyI GRCGYC 1 cut(s) 694
AdeI CACNNNGTG 1 cut(s) 1697
AfeI AGCGCT 1 cut(s) 1173
AfiI CCNNNNNNNGG 6 cut(s) 258, 736, 919, 1285, 2203, 2607
AflIII ACRYGT 2 cut(s) 1623, 2043
AgeI ACCGGT 3 cut(s) 65, 572, 866
AhlI ACTAGT 1 cut(s) 2564
AjiI CACGTC 3 cut(s) 1506, 1747, 1960
AjnI CCWGG 1 cut(s) 951
AjuI GAANNNNNNNTTGG 4 cut(s) 359, 391, 1964, 1996
AleI CACNNNNGTG 1 cut(s) 1929
Alw21I GWGCWC 3 cut(s) 816, 1319, 1970
Alw26I GTCTC 6 cut(s) 479, 490, 952, 1128, 1764, 1950
AlwI GGATC 3 cut(s) 929, 2188, 2636
Ama87I CYCGRG 1 cut(s) 500
Aor51HI AGCGCT 1 cut(s) 1173
AoxI GGCC 6 cut(s) 124, 304, 1026, 1425, 1998, 2183
ApeKI GCWGC 4 cut(s) 590, 1169, 1346, 2228
ApoI RAATTY 6 cut(s) 176, 275, 311, 467, 2284, 2581
AsiGI ACCGGT 3 cut(s) 65, 572, 866
Asp718I GGTACC 2 cut(s) 1303, 1634
AspLEI GCGC 5 cut(s) 197, 1174, 1651, 1906, 2398
AspS9I GGNCC 2 cut(s) 1265, 1824
AsuC2I CCSGG 5 cut(s) 303, 350, 1372, 2099, 2458
AsuNHI GCTAGC 1 cut(s) 233
AvaI CYCGRG 1 cut(s) 500
AvaII GGWCC 2 cut(s) 1265, 1824
BaeGI GKGCMC 2 cut(s) 1702, 2036
BaeI ACNNNNGTAYC 2 cut(s) 1618, 1651
BalI TGGCCA 2 cut(s) 1028, 2000
BanI GGYRCC 4 cut(s) 623, 1303, 1634, 1697
BanII GRGCYC 1 cut(s) 816
BauI CACGAG 1 cut(s) 1754
BbsI GAAGAC 4 cut(s) 720, 1104, 2532, 2617
Bbv12I GWGCWC 3 cut(s) 816, 1319, 1970
BbvI GCAGC 4 cut(s) 602, 1181, 1358, 2215
BccI CCATC 6 cut(s) 661, 707, 902, 1217, 1646, 1758
BceAI ACGGC 4 cut(s) 205, 637, 1510, 1688
BcgI CGANNNNNNTGC 4 cut(s) 204, 238, 2418, 2452
BciT130I CCWGG 1 cut(s) 953
BclI TGATCA 1 cut(s) 1944
BcnI CCSGG 5 cut(s) 303, 350, 1372, 2099, 2458
BcoDI GTCTC 6 cut(s) 479, 490, 952, 1128, 1764, 1950
BcuI ACTAGT 1 cut(s) 2564
BfaI CTAG 8 cut(s) 234, 1160, 1175, 1223, 2181, 2225, 2565, 2592
BfoI RGCGCY 2 cut(s) 1175, 2399
BglII AGATCT 1 cut(s) 2634
BisI GCNGC 7 cut(s) 390, 591, 1170, 1347, 1569, 1902, 2229
BlpI GCTNAGC 1 cut(s) 1238
BlsI GCNGC 7 cut(s) 391, 592, 1171, 1348, 1570, 1903, 2230
BmcAI AGTACT 2 cut(s) 1164, 2105
Bme1390I CCNGG 6 cut(s) 303, 350, 953, 1372, 2099, 2458
Bme18I GGWCC 2 cut(s) 1265, 1824
BmeT110I CYCGRG 1 cut(s) 500
BmgBI CACGTC 3 cut(s) 1506, 1747, 1960
BmgT120I GGNCC 2 cut(s) 1265, 1824
BmiI GGNNCC 8 cut(s) 625, 1267, 1305, 1636, 1699, 1825, 1826, 2539
BmrFI CCNGG 6 cut(s) 303, 350, 953, 1372, 2099, 2458
BmsI GCATC 3 cut(s) 206, 517, 1195
BmtI GCTAGC 1 cut(s) 237
BpiI GAAGAC 4 cut(s) 720, 1104, 2532, 2617
BpmI CTGGAG 1 cut(s) 683
Bpu1102I GCTNAGC 1 cut(s) 1238
BpuEI CTTGAG 2 cut(s) 1426, 1724
BpuMI CCSGG 5 cut(s) 303, 350, 1372, 2099, 2458
BsaHI GRCGYC 1 cut(s) 694
BsaJI CCNNGG 5 cut(s) 348, 1428, 1868, 2001, 2067
BsaWI WCCGGW 8 cut(s) 65, 88, 572, 719, 729, 866, 1126, 1285
BsaXI ACNNNNNCTCC 2 cut(s) 656, 686
Bsc4I CCNNNNNNNGG 6 cut(s) 258, 736, 919, 1285, 2203, 2607
Bse118I RCCGGY 5 cut(s) 65, 392, 572, 866, 2185
Bse1I ACTGG 3 cut(s) 1088, 1320, 1933
Bse3DI GCAATG 1 cut(s) 1353
BseBI CCWGG 1 cut(s) 953
BseDI CCNNGG 5 cut(s) 348, 1428, 1868, 2001, 2067
BseGI GGATG 5 cut(s) 45, 197, 315, 769, 888
BseLI CCNNNNNNNGG 6 cut(s) 258, 736, 919, 1285, 2203, 2607
BseMI GCAATG 1 cut(s) 1353
BseMII CTCAG 3 cut(s) 1252, 2118, 2622
BseNI ACTGG 3 cut(s) 1088, 1320, 1933
BseRI GAGGAG 2 cut(s) 9, 395
BseSI GKGCMC 2 cut(s) 1702, 2036
BseXI GCAGC 4 cut(s) 602, 1181, 1358, 2215
Bsh1236I CGCG 2 cut(s) 1833, 1906
Bsh1285I CGRYCG 4 cut(s) 93, 645, 871, 2372
BshFI GGCC 6 cut(s) 126, 306, 1028, 1427, 2000, 2185
BshNI GGYRCC 4 cut(s) 623, 1303, 1634, 1697
BshTI ACCGGT 3 cut(s) 65, 572, 866
BsiEI CGRYCG 4 cut(s) 93, 645, 871, 2372
BsiHKAI GWGCWC 3 cut(s) 816, 1319, 1970
BsiHKCI CYCGRG 1 cut(s) 500
BsiWI CGTACG 1 cut(s) 531
BslFI GGGAC 3 cut(s) 227, 436, 1837
BslI CCNNNNNNNGG 6 cut(s) 258, 736, 919, 1285, 2203, 2607
BsmAI GTCTC 6 cut(s) 479, 490, 952, 1128, 1764, 1950
BsmBI CGTCTC 1 cut(s) 1950
BsmFI GGGAC 3 cut(s) 227, 436, 1837
BsnI GGCC 6 cut(s) 126, 306, 1028, 1427, 2000, 2185
BsoBI CYCGRG 1 cut(s) 500
Bsp1286I GDGCHC 5 cut(s) 816, 1319, 1702, 1970, 2036
Bsp1407I TGTACA 2 cut(s) 1626, 1842
Bsp1720I GCTNAGC 1 cut(s) 1238
Bsp19I CCATGG 1 cut(s) 2067
BspANI GGCC 6 cut(s) 126, 306, 1028, 1427, 2000, 2185
BspCNI CTCAG 3 cut(s) 1251, 2119, 2623
BspFNI CGCG 2 cut(s) 1833, 1906
BspHI TCATGA 1 cut(s) 2248
BspLI GGNNCC 8 cut(s) 625, 1267, 1305, 1636, 1699, 1825, 1826, 2539
BspOI GCTAGC 1 cut(s) 237
BspPI GGATC 3 cut(s) 929, 2188, 2636
BspQI GCTCTTC 2 cut(s) 1307, 1958
BspT107I GGYRCC 4 cut(s) 623, 1303, 1634, 1697
BsrDI GCAATG 1 cut(s) 1353
BsrFI RCCGGY 5 cut(s) 65, 392, 572, 866, 2185
BsrGI TGTACA 2 cut(s) 1626, 1842
BsrI ACTGG 3 cut(s) 1088, 1320, 1933
BssAI RCCGGY 5 cut(s) 65, 392, 572, 866, 2185
BssECI CCNNGG 5 cut(s) 348, 1428, 1868, 2001, 2067
BssNAI GTATAC 1 cut(s) 1191
BssNI GRCGYC 1 cut(s) 694
BssSI CACGAG 1 cut(s) 1754
BssT1I CCWWGG 4 cut(s) 1428, 1868, 2001, 2067
Bst1107I GTATAC 1 cut(s) 1191
Bst2BI CACGAG 1 cut(s) 1754
Bst2UI CCWGG 1 cut(s) 953
Bst4CI ACNGT 8 cut(s) 1514, 1646, 1775, 1787, 2111, 2121, 2373, 2623
Bst6I CTCTTC 6 cut(s) 62, 380, 1129, 1307, 1869, 1958
BstACI GRCGYC 1 cut(s) 694
BstAUI TGTACA 2 cut(s) 1626, 1842
BstC8I GCNNGC 5 cut(s) 235, 394, 631, 1360, 2187
BstDEI CTNAG 6 cut(s) 931, 1238, 1485, 2049, 2127, 2631
BstDSI CCRYGG 1 cut(s) 2067
BstEII GGTNACC 1 cut(s) 2554
BstF5I GGATG 5 cut(s) 45, 197, 315, 769, 888
BstFNI CGCG 2 cut(s) 1833, 1906
BstH2I RGCGCY 2 cut(s) 1175, 2399
BstHHI GCGC 5 cut(s) 197, 1174, 1651, 1906, 2398
BstMAI GTCTC 6 cut(s) 479, 490, 952, 1128, 1764, 1950
BstMCI CGRYCG 4 cut(s) 93, 645, 871, 2372
BstMWI GCNNNNNNNGC 9 cut(s) 123, 630, 851, 1355, 1531, 1912, 2006, 2085, 2186
BstNI CCWGG 1 cut(s) 953
BstNSI RCATGY 2 cut(s) 1117, 2047
BstPI GGTNACC 1 cut(s) 2554
BstSCI CCNGG 6 cut(s) 301, 348, 951, 1370, 2097, 2456
BstSLI GKGCMC 2 cut(s) 1702, 2036
BstUI CGCG 2 cut(s) 1833, 1906
BstV1I GCAGC 4 cut(s) 602, 1181, 1358, 2215
BstV2I GAAGAC 4 cut(s) 720, 1104, 2532, 2617
BstX2I RGATCY 2 cut(s) 934, 2634
BstXI CCANNNNNNTGG 1 cut(s) 1070
BstYI RGATCY 2 cut(s) 934, 2634
BstZ17I GTATAC 1 cut(s) 1191
BsuRI GGCC 6 cut(s) 126, 306, 1028, 1427, 2000, 2185
BtgI CCRYGG 1 cut(s) 2067
BtrI CACGTC 3 cut(s) 1506, 1747, 1960
BtsCI GGATG 5 cut(s) 45, 197, 315, 769, 888
BtsIMutI CAGTG 2 cut(s) 1926, 2490
Cac8I GCNNGC 5 cut(s) 235, 394, 631, 1360, 2187
CciI TCATGA 1 cut(s) 2248
CfoI GCGC 5 cut(s) 197, 1174, 1651, 1906, 2398
Cfr10I RCCGGY 5 cut(s) 65, 392, 572, 866, 2185
Cfr13I GGNCC 2 cut(s) 1265, 1824
CseI GACGC 2 cut(s) 446, 702
CsiI ACCWGGT 1 cut(s) 951
CspAI ACCGGT 3 cut(s) 65, 572, 866
CspCI CAANNNNNGTGG 4 cut(s) 1436, 1471, 1642, 1677
DdeI CTNAG 6 cut(s) 931, 1238, 1485, 2049, 2127, 2631
DraIII CACNNNGTG 1 cut(s) 1697
DrdI GACNNNNNNGTC 4 cut(s) 101, 1486, 2044, 2131
DseDI GACNNNNNNGTC 4 cut(s) 101, 1486, 2044, 2131
EaeI YGGCCR 4 cut(s) 124, 304, 1026, 1998
Eam1104I CTCTTC 6 cut(s) 62, 380, 1129, 1307, 1869, 1958
EarI CTCTTC 6 cut(s) 62, 380, 1129, 1307, 1869, 1958
EciI GGCGGA 1 cut(s) 1571
Ecl136II GAGCTC 1 cut(s) 814
Eco130I CCWWGG 4 cut(s) 1428, 1868, 2001, 2067
Eco24I GRGCYC 1 cut(s) 816
Eco32I GATATC 2 cut(s) 166, 754
Eco47I GGWCC 2 cut(s) 1265, 1824
Eco47III AGCGCT 1 cut(s) 1173
Eco53kI GAGCTC 1 cut(s) 814
Eco57I CTGAAG 1 cut(s) 2466
Eco88I CYCGRG 1 cut(s) 500
Eco91I GGTNACC 1 cut(s) 2554
EcoICRI GAGCTC 1 cut(s) 814
EcoO109I RGGNCCY 1 cut(s) 1824
EcoO65I GGTNACC 1 cut(s) 2554
EcoRI GAATTC 1 cut(s) 467
EcoRII CCWGG 1 cut(s) 951
EcoRV GATATC 2 cut(s) 166, 754
EcoT14I CCWWGG 4 cut(s) 1428, 1868, 2001, 2067
EcoT22I ATGCAT 1 cut(s) 2352
EcoT38I GRGCYC 1 cut(s) 816
ErhI CCWWGG 4 cut(s) 1428, 1868, 2001, 2067
Esp3I CGTCTC 1 cut(s) 1950
FaqI GGGAC 3 cut(s) 227, 436, 1837
FauI CCCGC 2 cut(s) 462, 1604
FbaI TGATCA 1 cut(s) 1944
FblI GTMKAC 4 cut(s) 93, 618, 1190, 1334
Fnu4HI GCNGC 7 cut(s) 390, 591, 1170, 1347, 1569, 1902, 2229
FokI GGATG 5 cut(s) 32, 184, 302, 776, 895
FriOI GRGCYC 1 cut(s) 816
Fsp4HI GCNGC 7 cut(s) 390, 591, 1170, 1347, 1569, 1902, 2229
FspBI CTAG 8 cut(s) 234, 1160, 1175, 1223, 2181, 2225, 2565, 2592
GlaI GCGC 5 cut(s) 196, 1173, 1650, 1905, 2397
GluI GCNGC 7 cut(s) 390, 591, 1170, 1347, 1569, 1902, 2229
GsuI CTGGAG 1 cut(s) 683
HaeII RGCGCY 2 cut(s) 1175, 2399
HaeIII GGCC 6 cut(s) 126, 306, 1028, 1427, 2000, 2185
HgaI GACGC 2 cut(s) 446, 702
HhaI GCGC 5 cut(s) 197, 1174, 1651, 1906, 2398
Hin1I GRCGYC 1 cut(s) 694
Hin6I GCGC 5 cut(s) 195, 1172, 1649, 1904, 2396
HinP1I GCGC 5 cut(s) 195, 1172, 1649, 1904, 2396
HincII GTYRAC 5 cut(s) 94, 619, 1150, 2117, 2124
HindII GTYRAC 5 cut(s) 94, 619, 1150, 2117, 2124
HpaI GTTAAC 1 cut(s) 1150
Hpy99I CGWCG 5 cut(s) 226, 440, 623, 752, 2115
HpyAV CCTTC 3 cut(s) 520, 1931, 2458
HpyCH4III ACNGT 8 cut(s) 1514, 1646, 1775, 1787, 2111, 2121, 2373, 2623
HpyCH4IV ACGT 7 cut(s) 684, 1104, 1505, 1596, 1623, 1746, 1959
HpyCH4V TGCA 7 cut(s) 215, 1358, 1915, 2231, 2336, 2350, 2408
HpyF10VI GCNNNNNNNGC 9 cut(s) 123, 630, 851, 1355, 1531, 1912, 2006, 2085, 2186
HpyF3I CTNAG 6 cut(s) 931, 1238, 1485, 2049, 2127, 2631
HpySE526I ACGT 7 cut(s) 684, 1104, 1505, 1596, 1623, 1746, 1959
Hsp92I GRCGYC 1 cut(s) 694
HspAI GCGC 5 cut(s) 195, 1172, 1649, 1904, 2396
KflI GGGWCCC 1 cut(s) 1824
KpnI GGTACC 2 cut(s) 1307, 1638
KroI GCCGGC 2 cut(s) 392, 2185
KroNI GCCGGC 2 cut(s) 394, 2187
Ksp22I TGATCA 1 cut(s) 1944
KspAI GTTAAC 1 cut(s) 1150
LguI GCTCTTC 2 cut(s) 1307, 1958
LmnI GCTCC 2 cut(s) 275, 1073
Lsp1109I GCAGC 4 cut(s) 602, 1181, 1358, 2215
LweI GCATC 3 cut(s) 206, 517, 1195
MabI ACCWGGT 1 cut(s) 951
MaeI CTAG 8 cut(s) 234, 1160, 1175, 1223, 2181, 2225, 2565, 2592
MaeII ACGT 7 cut(s) 684, 1104, 1505, 1596, 1623, 1746, 1959
MfeI CAATTG 1 cut(s) 186
MflI RGATCY 2 cut(s) 934, 2634
MhlI GDGCHC 5 cut(s) 816, 1319, 1702, 1970, 2036
MlsI TGGCCA 2 cut(s) 1028, 2000
MluNI TGGCCA 2 cut(s) 1028, 2000
MlyI GAGTC 8 cut(s) 164, 458, 492, 954, 1238, 1243, 1694, 2522
MmeI TCCRAC 3 cut(s) 382, 1111, 1962
Mox20I TGGCCA 2 cut(s) 1028, 2000
Mph1103I ATGCAT 1 cut(s) 2352
MroNI GCCGGC 2 cut(s) 392, 2185
MscI TGGCCA 2 cut(s) 1028, 2000
MseI TTAA 4 cut(s) 945, 1149, 2411, 2604
MslI CAYNNNNRTG 5 cut(s) 722, 969, 1118, 1929, 2355
Msp20I TGGCCA 2 cut(s) 1028, 2000
MspA1I CMGCKG 2 cut(s) 457, 2204
MspR9I CCNGG 6 cut(s) 303, 350, 953, 1372, 2099, 2458
MunI CAATTG 1 cut(s) 186
MvaI CCWGG 1 cut(s) 953
MvnI CGCG 2 cut(s) 1833, 1906
MwoI GCNNNNNNNGC 9 cut(s) 123, 630, 851, 1355, 1531, 1912, 2006, 2085, 2186
NaeI GCCGGC 2 cut(s) 394, 2187
NciI CCSGG 5 cut(s) 303, 350, 1372, 2099, 2458
NcoI CCATGG 1 cut(s) 2067
NgoMIV GCCGGC 2 cut(s) 392, 2185
NheI GCTAGC 1 cut(s) 233
NlaIV GGNNCC 8 cut(s) 625, 1267, 1305, 1636, 1699, 1825, 1826, 2539
NmeAIII GCCGAG 1 cut(s) 238
NmuCI GTSAC 7 cut(s) 61, 382, 427, 1689, 1742, 1775, 2554
NsiI ATGCAT 1 cut(s) 2352
NspI RCATGY 2 cut(s) 1117, 2047
OliI CACNNNNGTG 1 cut(s) 1929
PaeR7I CTCGAG 1 cut(s) 500
PagI TCATGA 1 cut(s) 2248
PciI ACATGT 1 cut(s) 2043
PciSI GCTCTTC 2 cut(s) 1307, 1958
PcsI WCGNNNNNNNCGW 5 cut(s) 99, 444, 1101, 1752, 2091
PdiI GCCGGC 2 cut(s) 394, 2187
PfeI GAWTC 9 cut(s) 203, 549, 602, 678, 980, 1084, 1255, 1589, 2597
Pfl23II CGTACG 1 cut(s) 531
PinAI ACCGGT 3 cut(s) 65, 572, 866
PkrI GCNGC 7 cut(s) 391, 592, 1171, 1348, 1570, 1903, 2230
Ple19I CGATCG 1 cut(s) 645
PleI GAGTC 8 cut(s) 164, 457, 492, 954, 1238, 1243, 1693, 2522
PpsI GAGTC 8 cut(s) 164, 457, 492, 954, 1238, 1243, 1693, 2522
PpuMI RGGWCCY 1 cut(s) 1824
PscI ACATGT 1 cut(s) 2043
Psp124BI GAGCTC 1 cut(s) 816
Psp1406I AACGTT 1 cut(s) 1596
Psp5II RGGWCCY 1 cut(s) 1824
Psp6I CCWGG 1 cut(s) 951
PspEI GGTNACC 1 cut(s) 2554
PspGI CCWGG 1 cut(s) 951
PspLI CGTACG 1 cut(s) 531
PspN4I GGNNCC 8 cut(s) 625, 1267, 1305, 1636, 1699, 1825, 1826, 2539
PspPI GGNCC 2 cut(s) 1265, 1824
PspPPI RGGWCCY 1 cut(s) 1824
PsuI RGATCY 2 cut(s) 934, 2634
PvuI CGATCG 1 cut(s) 645
RseI CAYNNNNRTG 5 cut(s) 722, 969, 1118, 1929, 2355
SacI GAGCTC 1 cut(s) 816
SalI GTCGAC 2 cut(s) 92, 617
SapI GCTCTTC 2 cut(s) 1307, 1958
SaqAI TTAA 4 cut(s) 945, 1149, 2411, 2604
SatI GCNGC 7 cut(s) 390, 591, 1170, 1347, 1569, 1902, 2229
Sau96I GGNCC 2 cut(s) 1265, 1824
ScaI AGTACT 2 cut(s) 1164, 2105
SchI GAGTC 8 cut(s) 164, 458, 492, 954, 1238, 1243, 1694, 2522
ScrFI CCNGG 6 cut(s) 303, 350, 953, 1372, 2099, 2458
SduI GDGCHC 5 cut(s) 816, 1319, 1702, 1970, 2036
SexAI ACCWGGT 1 cut(s) 951
SfaNI GCATC 3 cut(s) 206, 517, 1195
Sfr274I CTCGAG 1 cut(s) 500
SinI GGWCC 2 cut(s) 1265, 1824
SlaI CTCGAG 1 cut(s) 500
SmiMI CAYNNNNRTG 5 cut(s) 722, 969, 1118, 1929, 2355
SmlI CTYRAG 3 cut(s) 500, 1441, 1703
SmoI CTYRAG 3 cut(s) 500, 1441, 1703
SpeI ACTAGT 1 cut(s) 2564
SspMI CTAG 8 cut(s) 234, 1160, 1175, 1223, 2181, 2225, 2565, 2592
SstI GAGCTC 1 cut(s) 816
StyD4I CCNGG 6 cut(s) 301, 348, 951, 1370, 2097, 2456
StyI CCWWGG 4 cut(s) 1428, 1868, 2001, 2067
TaaI ACNGT 8 cut(s) 1514, 1646, 1775, 1787, 2111, 2121, 2373, 2623
TaiI ACGT 7 cut(s) 687, 1107, 1508, 1599, 1626, 1749, 1962
TaqII GACCGA 1 cut(s) 861
TatI WGTACW 5 cut(s) 1162, 1626, 1842, 2103, 2142
TauI GCSGC 3 cut(s) 392, 1571, 1904
TfiI GAWTC 9 cut(s) 203, 549, 602, 678, 980, 1084, 1255, 1589, 2597
Tru1I TTAA 4 cut(s) 945, 1149, 2411, 2604
Tru9I TTAA 4 cut(s) 945, 1149, 2411, 2604
TscAI CASTG 2 cut(s) 1933, 2490
TseFI GTSAC 7 cut(s) 61, 382, 427, 1689, 1742, 1775, 2554
TseI GCWGC 4 cut(s) 590, 1169, 1346, 2228
Tsp45I GTSAC 7 cut(s) 61, 382, 427, 1689, 1742, 1775, 2554
TspGWI ACGGA 7 cut(s) 370, 415, 561, 1730, 1730, 2186, 2271
TspRI CASTG 2 cut(s) 1933, 2490
VpaK11BI GGWCC 2 cut(s) 1265, 1824
XapI RAATTY 6 cut(s) 176, 275, 311, 467, 2284, 2581
XceI RCATGY 2 cut(s) 1117, 2047
XcmI CCANNNNNNNNNTGG 1 cut(s) 1866
XhoI CTCGAG 1 cut(s) 500
XmiI GTMKAC 4 cut(s) 93, 618, 1190, 1334
XspI CTAG 8 cut(s) 234, 1160, 1175, 1223, 2181, 2225, 2565, 2592
ZrmI AGTACT 2 cut(s) 1164, 2105
Zsp2I ATGCAT 1 cut(s) 2352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.