MD09G1022200.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002827
Physical Location & Seq
Forward (+)
1338369 .. 1340773
2405 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1022200.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGGAGTTTTGGAAACCGCTTCTGGCATCTGGGTTCTTCTTGGCTTTCTGGGTTTTGGCTGTGGGAGGCCAGAGCTTACCACCCTGTTCTTTTCCGGCAATTTACAACTTCGGGGACTCGAATTCCGACACCGGCGGCATATCGGCCGCGTTCGAGCCAATCAAAGCTCCTTACGGCGAGGCATTCTTCCACAAACCGGCCGGGAGGGACGCCGATGGCCGCCTTATCATAGACTTCTTAGCTGAGCGCCTTAAGTTGCCATACTTGAGTGCATATTTGAACTCACTTGGAACAAATTACCGGCACGGTGCGAATTTCGCCACCGGAGGATCCACCATCAGGCGGCCGAACGAAACCATTTTCCAGTACGGCATTAGCCCTTTCTCCCTCGATATGCAGACGGCGCAGTTCCTGCAGTTCAAAGCTCGCACTGCCGATCTCTATCGCCAAGCCAAGAACCCTTCTGAGAGAAGCGCTTTGCCGGATCCACGGGAGTTCGCAGAGGCTCTGTACACGTTTGATATCGGGCAAAACGACCTTTCCATGGGTTTTCGGAAGCTCAGTTTCGACCAGTTTCGTGCAGAACTGCCGGACATTGTCAACCAGTTAGCCACAGCAGTCCATCGGATATATGAAGAAGGGGGGAGGACGTTTTGGATACACAACACAGGTCCTGTTGGTTGCTTGCCGGTACAATTATTCTACAACCTGAATCCGCCGGCCGGTTATCTCGACGACCACGGCTGCGTCAAGGCCCAAAACGACATCGCCGTAGAGTTCAACACGCAGCTCAGAGACAAAGTCATCAAACTAAGAGCTGAGCTGCCACAAGCCGCAATCACATACGTCGATGTCTACGCCGCCAAGTACGGACTCATCGGCACTGCAAAGACTGAAGGGTTTGCTGATCCGATGAAGGTTTGTTGCGGTTATCACGTGAGATACGTCCACATCTGGTGCGGGAACAAGGGAAGCGTAAATGGAAGCGATGTCTACGGTGCTTCTTGTCAAAACCCGTCGGCTTTCATTAGCTGGGATGGAGTTCACTACACCGAGGGAGCGAACCAGTGGGTTGCTAATCGTATACTCAATGGCTCGCCATCGTCGAACCCGGCAATTCCTATTACTCAAGCTTGTCATAGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

383

Amino Acids

42.25

Weight (kDa)

6.55

Isoelectric Point (pI)

28.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 362 4.8e-44 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 855, 993, 1084
AccII CGCG 1 cut(s) 149
AclWI GGATC 5 cut(s) 324, 337, 479, 492, 902
AcoI YGGCCR 5 cut(s) 144, 198, 217, 344, 720
AcsI RAATTY 2 cut(s) 121, 313
AcuI CTGAAG 1 cut(s) 915
AcvI CACGTG 1 cut(s) 937
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 4 cut(s) 368, 512, 693, 869
AfeI AGCGCT 1 cut(s) 475
AfiI CCNNNNNNNGG 5 cut(s) 196, 339, 342, 544, 722
AflII CTTAAG 1 cut(s) 251
AflIII ACRYGT 1 cut(s) 513
AgsI TTSAA 3 cut(s) 280, 421, 781
Alw26I GTCTC 1 cut(s) 789
AlwI GGATC 5 cut(s) 324, 337, 479, 492, 902
AlwNI CAGNNNCTG 2 cut(s) 412, 674
Aor51HI AGCGCT 1 cut(s) 475
AoxI GGCC 7 cut(s) 67, 144, 198, 217, 344, 720, 753
ApeKI GCWGC 3 cut(s) 744, 787, 823
ApoI RAATTY 2 cut(s) 121, 313
AspLEI GCGC 3 cut(s) 249, 406, 476
AspS9I GGNCC 2 cut(s) 671, 754
AsuC2I CCSGG 2 cut(s) 202, 1112
AvaII GGWCC 1 cut(s) 671
BamHI GGATCC 2 cut(s) 329, 484
BbrPI CACGTG 1 cut(s) 937
BbvI GCAGC 3 cut(s) 731, 799, 810
BccI CCATC 5 cut(s) 209, 344, 630, 1031, 1108
BceAI ACGGC 5 cut(s) 190, 385, 417, 755, 757
BciVI GTATCC 1 cut(s) 651
BcnI CCSGG 2 cut(s) 202, 1112
BcoDI GTCTC 1 cut(s) 789
BfmI CTRYAG 1 cut(s) 413
BfoI RGCGCY 2 cut(s) 250, 477
BfrI CTTAAG 1 cut(s) 251
BfuI GTATCC 1 cut(s) 651
BisI GCNGC 9 cut(s) 136, 147, 220, 344, 745, 788, 824, 834, 861
BlpI GCTNAGC 2 cut(s) 243, 819
BlsI GCNGC 9 cut(s) 137, 148, 221, 345, 746, 789, 825, 835, 862
Bme1390I CCNGG 2 cut(s) 202, 1112
Bme18I GGWCC 1 cut(s) 671
BmgT120I GGNCC 2 cut(s) 671, 754
BmiI GGNNCC 2 cut(s) 331, 486
BmrFI CCNGG 2 cut(s) 202, 1112
BmsI GCATC 1 cut(s) 35
Bpu1102I GCTNAGC 2 cut(s) 243, 819
BpuEI CTTGAG 2 cut(s) 286, 1113
BpuMI CCSGG 2 cut(s) 202, 1112
BsaAI YACGTR 1 cut(s) 937
BsaBI GATNNNNATC 1 cut(s) 441
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 4 cut(s) 488, 543, 739, 1053
BsaWI WCCGGW 1 cut(s) 323
Bsc4I CCNNNNNNNGG 5 cut(s) 196, 339, 342, 544, 722
Bse118I RCCGGY 6 cut(s) 131, 196, 300, 688, 718, 722
Bse1I ACTGG 4 cut(s) 364, 571, 604, 1066
Bse8I GATNNNNATC 1 cut(s) 441
BseDI CCNNGG 4 cut(s) 488, 543, 739, 1053
BseGI GGATG 1 cut(s) 1042
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 5 cut(s) 196, 339, 342, 544, 722
BseMII CTCAG 5 cut(s) 234, 456, 574, 805, 810
BseNI ACTGG 4 cut(s) 364, 571, 604, 1066
BseX3I CGGCCG 4 cut(s) 144, 198, 344, 720
BseXI GCAGC 3 cut(s) 731, 799, 810
BseYI CCCAGC 1 cut(s) 1032
BsgI GTGCAG 1 cut(s) 600
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 4 cut(s) 147, 201, 347, 723
BshFI GGCC 7 cut(s) 69, 146, 200, 219, 346, 722, 755
BsiEI CGRYCG 4 cut(s) 147, 201, 347, 723
BslFI GGGAC 2 cut(s) 128, 221
BslI CCNNNNNNNGG 5 cut(s) 196, 339, 342, 544, 722
BsmAI GTCTC 1 cut(s) 789
BsmFI GGGAC 2 cut(s) 128, 221
BsmI GAATGC 1 cut(s) 182
BsnI GGCC 7 cut(s) 69, 146, 200, 219, 346, 722, 755
Bsp1407I TGTACA 1 cut(s) 510
Bsp143I GATC 4 cut(s) 329, 436, 484, 907
Bsp1720I GCTNAGC 2 cut(s) 243, 819
Bsp19I CCATGG 1 cut(s) 543
BspANI GGCC 7 cut(s) 69, 146, 200, 219, 346, 722, 755
BspCNI CTCAG 5 cut(s) 235, 457, 573, 804, 811
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 2 cut(s) 331, 486
BspMAI CTGCAG 1 cut(s) 417
BspPI GGATC 5 cut(s) 324, 337, 479, 492, 902
BspTI CTTAAG 1 cut(s) 251
BsrFI RCCGGY 6 cut(s) 131, 196, 300, 688, 718, 722
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 4 cut(s) 364, 571, 604, 1066
BssAI RCCGGY 6 cut(s) 131, 196, 300, 688, 718, 722
BssECI CCNNGG 4 cut(s) 488, 543, 739, 1053
BssMI GATC 4 cut(s) 329, 436, 484, 907
BssNAI GTATAC 1 cut(s) 1085
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 543
Bst1107I GTATAC 1 cut(s) 1085
Bst4CI ACNGT 2 cut(s) 308, 998
BstACI GRCGYC 1 cut(s) 210
BstAFI CTTAAG 1 cut(s) 251
BstAPI GCANNNNNTGC 1 cut(s) 412
BstAUI TGTACA 1 cut(s) 510
BstBAI YACGTR 1 cut(s) 937
BstC8I GCNNGC 4 cut(s) 427, 686, 720, 1097
BstDEI CTNAG 7 cut(s) 238, 243, 465, 560, 791, 812, 819
BstDSI CCRYGG 3 cut(s) 488, 543, 739
BstF5I GGATG 1 cut(s) 1042
BstFNI CGCG 1 cut(s) 149
BstH2I RGCGCY 2 cut(s) 250, 477
BstHHI GCGC 3 cut(s) 249, 406, 476
BstKTI GATC 4 cut(s) 332, 439, 487, 910
BstMAI GTCTC 1 cut(s) 789
BstMBI GATC 4 cut(s) 329, 436, 484, 907
BstMCI CGRYCG 4 cut(s) 147, 201, 347, 723
BstMWI GCNNNNNNNGC 4 cut(s) 317, 403, 412, 431
BstSCI CCNGG 2 cut(s) 200, 1110
BstSFI CTRYAG 1 cut(s) 413
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 3 cut(s) 731, 799, 810
BstX2I RGATCY 2 cut(s) 329, 484
BstYI RGATCY 2 cut(s) 329, 484
BstZ17I GTATAC 1 cut(s) 1085
BstZI CGGCCG 4 cut(s) 144, 198, 344, 720
BsuI GTATCC 1 cut(s) 651
BsuRI GGCC 7 cut(s) 69, 146, 200, 219, 346, 722, 755
BtgI CCRYGG 3 cut(s) 488, 543, 739
BtgZI GCGATG 2 cut(s) 751, 1002
BtsCI GGATG 1 cut(s) 1042
BtsI GCAGTG 2 cut(s) 429, 882
BtsIMutI CAGTG 3 cut(s) 429, 882, 1073
Cac8I GCNNGC 4 cut(s) 427, 686, 720, 1097
CaiI CAGNNNCTG 2 cut(s) 412, 674
CfoI GCGC 3 cut(s) 249, 406, 476
Cfr10I RCCGGY 6 cut(s) 131, 196, 300, 688, 718, 722
Cfr13I GGNCC 2 cut(s) 671, 754
CseI GACGC 2 cut(s) 218, 736
Csp6I GTAC 4 cut(s) 367, 511, 692, 868
CviAII CATG 1 cut(s) 544
CviQI GTAC 4 cut(s) 367, 511, 692, 868
DdeI CTNAG 7 cut(s) 238, 243, 465, 560, 791, 812, 819
DpnI GATC 4 cut(s) 331, 438, 486, 909
DpnII GATC 4 cut(s) 329, 436, 484, 907
EaeI YGGCCR 5 cut(s) 144, 198, 217, 344, 720
EagI CGGCCG 4 cut(s) 144, 198, 344, 720
EciI GGCGGA 1 cut(s) 705
EclXI CGGCCG 4 cut(s) 144, 198, 344, 720
Eco130I CCWWGG 1 cut(s) 543
Eco32I GATATC 1 cut(s) 523
Eco47I GGWCC 1 cut(s) 671
Eco47III AGCGCT 1 cut(s) 475
Eco52I CGGCCG 4 cut(s) 144, 198, 344, 720
Eco57I CTGAAG 1 cut(s) 915
Eco72I CACGTG 1 cut(s) 937
EcoO109I RGGNCCY 1 cut(s) 671
EcoRI GAATTC 1 cut(s) 121
EcoRV GATATC 1 cut(s) 523
EcoT14I CCWWGG 1 cut(s) 543
ErhI CCWWGG 1 cut(s) 543
FaeI CATG 1 cut(s) 547
FaqI GGGAC 2 cut(s) 128, 221
FatI CATG 1 cut(s) 543
FauI CCCGC 1 cut(s) 953
FblI GTMKAC 3 cut(s) 855, 993, 1084
Fnu4HI GCNGC 9 cut(s) 136, 147, 220, 344, 745, 788, 824, 834, 861
FokI GGATG 1 cut(s) 1049
Fsp4HI GCNGC 9 cut(s) 136, 147, 220, 344, 745, 788, 824, 834, 861
GlaI GCGC 3 cut(s) 248, 405, 475
GluI GCNGC 9 cut(s) 136, 147, 220, 344, 745, 788, 824, 834, 861
GsaI CCCAGC 1 cut(s) 1036
HaeII RGCGCY 2 cut(s) 250, 477
HaeIII GGCC 7 cut(s) 69, 146, 200, 219, 346, 722, 755
HgaI GACGC 2 cut(s) 218, 736
HhaI GCGC 3 cut(s) 249, 406, 476
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 1 cut(s) 547
Hin6I GCGC 3 cut(s) 247, 404, 474
HinP1I GCGC 3 cut(s) 247, 404, 474
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HindIII AAGCTT 1 cut(s) 1131
HinfI GANTC 3 cut(s) 116, 712, 873
Hpy166II GTNNAC 7 cut(s) 513, 601, 856, 949, 994, 1045, 1085
Hpy188I TCNGA 6 cut(s) 127, 466, 555, 627, 794, 912
Hpy188III TCNNGA 1 cut(s) 731
Hpy8I GTNNAC 7 cut(s) 513, 601, 856, 949, 994, 1045, 1085
Hpy99I CGWCG 4 cut(s) 737, 851, 1021, 1108
HpyAV CCTTC 4 cut(s) 471, 632, 890, 910
HpyCH4III ACNGT 2 cut(s) 308, 998
HpyCH4IV ACGT 5 cut(s) 515, 650, 846, 936, 945
HpyCH4V TGCA 5 cut(s) 272, 397, 415, 581, 887
HpyF10VI GCNNNNNNNGC 4 cut(s) 317, 403, 412, 431
HpyF3I CTNAG 7 cut(s) 238, 243, 465, 560, 791, 812, 819
HpySE526I ACGT 5 cut(s) 515, 650, 846, 936, 945
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 1 cut(s) 547
HspAI GCGC 3 cut(s) 247, 404, 474
KroI GCCGGC 1 cut(s) 718
KroNI GCCGGC 1 cut(s) 720
Kzo9I GATC 4 cut(s) 329, 436, 484, 907
LmnI GCTCC 2 cut(s) 172, 1058
Lsp1109I GCAGC 3 cut(s) 731, 799, 810
LweI GCATC 1 cut(s) 35
MaeII ACGT 5 cut(s) 515, 650, 846, 936, 945
MalI GATC 4 cut(s) 331, 438, 486, 909
MboI GATC 4 cut(s) 329, 436, 484, 907
MboII GAAGA 3 cut(s) 28, 178, 647
MflI RGATCY 2 cut(s) 329, 484
MluCI AATT 6 cut(s) 99, 121, 295, 313, 695, 1116
MlyI GAGTC 2 cut(s) 110, 867
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 8 cut(s) 59, 172, 198, 320, 398, 496, 639, 1048
MroNI GCCGGC 1 cut(s) 718
MseI TTAA 1 cut(s) 252
MspCI CTTAAG 1 cut(s) 251
MspR9I CCNGG 2 cut(s) 202, 1112
Mva1269I GAATGC 1 cut(s) 182
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 4 cut(s) 317, 403, 412, 431
NaeI GCCGGC 1 cut(s) 720
NciI CCSGG 2 cut(s) 202, 1112
NcoI CCATGG 1 cut(s) 543
NdeII GATC 4 cut(s) 329, 436, 484, 907
NgoMIV GCCGGC 1 cut(s) 718
NlaIII CATG 1 cut(s) 547
NlaIV GGNNCC 2 cut(s) 331, 486
PcsI WCGNNNNNNNCGW 4 cut(s) 531, 768, 942, 1103
PctI GAATGC 1 cut(s) 182
PdiI GCCGGC 1 cut(s) 720
PfeI GAWTC 1 cut(s) 712
PflFI GACNNNGTC 2 cut(s) 596, 800
PkrI GCNGC 9 cut(s) 137, 148, 221, 345, 746, 789, 825, 835, 862
PleI GAGTC 2 cut(s) 110, 867
PmaCI CACGTG 1 cut(s) 937
PmlI CACGTG 1 cut(s) 937
PpsI GAGTC 2 cut(s) 110, 867
Ppu21I YACGTR 1 cut(s) 937
PpuMI RGGWCCY 1 cut(s) 671
Psp5II RGGWCCY 1 cut(s) 671
PspCI CACGTG 1 cut(s) 937
PspFI CCCAGC 1 cut(s) 1032
PspN4I GGNNCC 2 cut(s) 331, 486
PspPI GGNCC 2 cut(s) 671, 754
PspPPI RGGWCCY 1 cut(s) 671
PstI CTGCAG 1 cut(s) 417
PstNI CAGNNNCTG 2 cut(s) 412, 674
PsuI RGATCY 2 cut(s) 329, 484
PsyI GACNNNGTC 2 cut(s) 596, 800
RsaI GTAC 4 cut(s) 368, 512, 693, 869
RsaNI GTAC 4 cut(s) 367, 511, 692, 868
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 9 cut(s) 136, 147, 220, 344, 745, 788, 824, 834, 861
Sau3AI GATC 4 cut(s) 329, 436, 484, 907
Sau96I GGNCC 2 cut(s) 671, 754
SchI GAGTC 2 cut(s) 110, 867
ScrFI CCNGG 2 cut(s) 202, 1112
SfaNI GCATC 1 cut(s) 35
SfcI CTRYAG 1 cut(s) 413
SgrAI CRCCGGYG 1 cut(s) 131
SinI GGWCC 1 cut(s) 671
SmlI CTYRAG 3 cut(s) 251, 265, 1128
SmoI CTYRAG 3 cut(s) 251, 265, 1128
Sse9I AATT 6 cut(s) 99, 121, 295, 313, 695, 1116
StyD4I CCNGG 2 cut(s) 200, 1110
StyI CCWWGG 1 cut(s) 543
TaaI ACNGT 2 cut(s) 308, 998
TaiI ACGT 5 cut(s) 518, 653, 849, 939, 948
TaqI TCGA 7 cut(s) 119, 153, 390, 567, 732, 849, 1106
TasI AATT 6 cut(s) 99, 121, 295, 313, 695, 1116
TatI WGTACW 1 cut(s) 510
TauI GCSGC 6 cut(s) 138, 149, 222, 346, 836, 863
TfiI GAWTC 1 cut(s) 712
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 3 cut(s) 436, 889, 1073
TseI GCWGC 3 cut(s) 744, 787, 823
TspDTI ATGAA 3 cut(s) 648, 929, 1015
TspGWI ACGGA 1 cut(s) 885
TspRI CASTG 3 cut(s) 436, 889, 1073
Tth111I GACNNNGTC 2 cut(s) 596, 800
Vha464I CTTAAG 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 671
XapI RAATTY 2 cut(s) 121, 313
XmiI GTMKAC 3 cut(s) 855, 993, 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.