FvH4_6g51910

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002827
Physical Location & Seq
Reverse (-)
38400721 .. 38402706
1986 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g51910.t3

Sequence Viewer

Length: 1212 bp
ATGTCCAAATGCCCTGCATATGTGTTACATTGTGCAGTAGAGAAAATGGAGACTGGAAGAGTTTTGGTTGCAAGCGTGTTGGTTATATATGCGCTAGTGATCAGTGTGGGAGGTGAAGAAGATGCTTCATCACCTTCGGTTTCGGCTTGTGAGTTTCCAGCAATCTATAACTTTGGAGACTCGAATTCAGATACCGGTGGTATATCTGCTGCATTCTACGCCATGCCGTCACCCTACGGCGACACTTTCTTCAACAGACCAGCTGGGAGGGGCAGTGATGGTCGTCTAATAATAGATTTCATAGCCGAGAAGCTGGGGTTGCCATACCTGAGTTCATATTTGGACTCAATTCGAAGCAATTTTCGACACGGTGCTAATTTTGCTTCTGGAGGAGCAACCATCAGGCGGCAGAATGAGTCATGGTTTGACAATGGTGTGAGTCCTTTTCCTCTTGACATTCAGTTTGTGCAGTATGATCAGTTCAAGGGGCGTACCACCACTTTTTACAACCAAGCTAAGGAACTTTTCGACACACAAGATCTCCCTAGACCTGAGGACTTCTCAAAGGCTCTTTACACTTTTGATATAGGCCAAAACGATCTAGCTGCTGGTTTTCGGAAAATGAGTACTGAGAAACTTCTAGCAGAAATTCCGGACATTGTTAACCAGTTGGCTGCAGCAATCCGAAGTTTGTACGACCGAGGGGCAAGGGCATTCTGGATACACAACACTGGTCCAATAGGTTGCTTGGGAGTGACATTACACTACATCCATGATCCGAGCCCTACCGGATACCTTGATCGCCACGGTTGCGTGAAGTTTCAAAACGACATGGCAAGACAGTTCAATAGGCTGCTCAAACAGAAAGTGATCCAACTAAGGAAGGAACTCCCTTTTTCTGCAATAACATATGTCAATGTCTTTGCTGCAAAGTATAAGCTCATCAGCAATGCAAAGAAGCTTGGGTTTTTCGATAAAACGAAGATCTGCTGTGGATACCATGAAGATTATGACCATGTGTGGTGTGGGAATAAGGGAAAGATAAATGGGAATGAAATATATGCAGGTTCGTGTGAAGATCCTTCCTTGTATATAAGCTGGGATGGTGTGCACTACACTGAGGCTGCAAATCATTGGATTGCTAATCAAATTGCCAGTGGTTCATTCTCAGATCCACCTGTTTCAATCACAAATGCATGTCATAGTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

404

Amino Acids

44.71

Weight (kDa)

6.26

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 55 - 229 4.2e-21 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1055
AccIII TCCGGA 1 cut(s) 652
AciI CCGC 1 cut(s) 406
AclWI GGATC 4 cut(s) 770, 865, 1073, 1166
AcsI RAATTY 2 cut(s) 184, 648
AfaI GTAC 3 cut(s) 493, 628, 695
AfiI CCNNNNNNNGG 2 cut(s) 405, 517
AgeI ACCGGT 1 cut(s) 194
AgsI TTSAA 5 cut(s) 253, 484, 824, 847, 1185
AluBI AGCT 7 cut(s) 263, 313, 515, 605, 940, 961, 1098
AluI AGCT 7 cut(s) 263, 313, 515, 605, 940, 961, 1098
Alw21I GWGCWC 1 cut(s) 1113
Alw26I GTCTC 2 cut(s) 44, 171
Alw44I GTGCAC 1 cut(s) 1109
AlwI GGATC 4 cut(s) 770, 865, 1073, 1166
Aor13HI TCCGGA 1 cut(s) 652
AoxI GGCC 1 cut(s) 589
ApaLI GTGCAC 1 cut(s) 1109
ApeKI GCWGC 7 cut(s) 209, 605, 674, 677, 853, 926, 1124
ApoI RAATTY 2 cut(s) 184, 648
ArsI GACNNNNNNTTYG 2 cut(s) 446, 478
AsiGI ACCGGT 1 cut(s) 194
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 1 cut(s) 734
AsuHPI GGTGA 3 cut(s) 123, 125, 222
AsuII TTCGAA 1 cut(s) 352
AvaII GGWCC 1 cut(s) 734
AxyI CCTNAGG 1 cut(s) 552
BaeGI GKGCMC 1 cut(s) 1113
BanII GRGCYC 1 cut(s) 785
Bbv12I GWGCWC 1 cut(s) 1113
BbvI GCAGC 7 cut(s) 196, 592, 661, 689, 840, 913, 1111
BccI CCATC 3 cut(s) 272, 407, 1097
BceAI ACGGC 2 cut(s) 211, 253
BcgI CGANNNNNNTGC 2 cut(s) 587, 621
BciVI GTATCC 3 cut(s) 714, 785, 989
BclI TGATCA 2 cut(s) 99, 475
BcoDI GTCTC 2 cut(s) 44, 171
BfaI CTAG 4 cut(s) 95, 546, 602, 641
BfmI CTRYAG 1 cut(s) 675
BfuAI ACCTGC 1 cut(s) 1055
BfuI GTATCC 3 cut(s) 714, 785, 989
BglII AGATCT 2 cut(s) 538, 984
BisI GCNGC 8 cut(s) 210, 407, 606, 675, 678, 854, 927, 1125
BlsI GCNGC 8 cut(s) 211, 408, 607, 676, 679, 855, 928, 1126
BmcAI AGTACT 1 cut(s) 628
Bme18I GGWCC 1 cut(s) 734
BmgT120I GGNCC 1 cut(s) 734
BmsI GCATC 1 cut(s) 112
BplI GAGNNNNNCTC 2 cut(s) 545, 577
BpmI CTGGAG 1 cut(s) 408
Bpu10I CCTNAGC 1 cut(s) 516
Bpu14I TTCGAA 1 cut(s) 352
BsaJI CCNNGG 2 cut(s) 700, 805
BsaWI WCCGGW 3 cut(s) 194, 652, 788
BsaXI ACNNNNNCTCC 4 cut(s) 259, 289, 525, 555
Bsc4I CCNNNNNNNGG 2 cut(s) 405, 517
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 4 cut(s) 58, 667, 736, 1155
Bse21I CCTNAGG 1 cut(s) 552
Bse3DI GCAATG 1 cut(s) 955
BseAI TCCGGA 1 cut(s) 652
BseDI CCNNGG 2 cut(s) 700, 805
BseGI GGATG 2 cut(s) 768, 1108
BseLI CCNNNNNNNGG 2 cut(s) 405, 517
BseMI GCAATG 1 cut(s) 955
BseMII CTCAG 5 cut(s) 320, 543, 621, 1110, 1182
BseNI ACTGG 4 cut(s) 58, 667, 736, 1155
BseRI GAGGAG 1 cut(s) 405
BseSI GKGCMC 1 cut(s) 1113
BseXI GCAGC 7 cut(s) 196, 592, 661, 689, 840, 913, 1111
BseYI CCCAGC 3 cut(s) 263, 313, 1098
BsgI GTGCAG 2 cut(s) 54, 488
Bsh1285I CGRYCG 1 cut(s) 700
BshFI GGCC 1 cut(s) 591
BshTI ACCGGT 1 cut(s) 194
BsiEI CGRYCG 1 cut(s) 700
BsiHKAI GWGCWC 1 cut(s) 1113
BsiSI CCGG 3 cut(s) 195, 653, 789
BslI CCNNNNNNNGG 2 cut(s) 405, 517
BsmAI GTCTC 2 cut(s) 44, 171
BsmI GAATGC 2 cut(s) 212, 713
BsnI GGCC 1 cut(s) 591
Bsp119I TTCGAA 1 cut(s) 352
Bsp1286I GDGCHC 2 cut(s) 785, 1113
Bsp13I TCCGGA 1 cut(s) 652
BspACI CCGC 1 cut(s) 406
BspANI GGCC 1 cut(s) 591
BspCNI CTCAG 5 cut(s) 321, 544, 622, 1111, 1181
BspEI TCCGGA 1 cut(s) 652
BspMAI CTGCAG 1 cut(s) 679
BspMI ACCTGC 1 cut(s) 1055
BspPI GGATC 4 cut(s) 770, 865, 1073, 1166
BspT104I TTCGAA 1 cut(s) 352
BsrDI GCAATG 1 cut(s) 955
BsrFI RCCGGY 1 cut(s) 194
BsrI ACTGG 4 cut(s) 58, 667, 736, 1155
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 2 cut(s) 700, 805
Bst4CI ACNGT 3 cut(s) 371, 809, 843
Bst6I CTCTTC 1 cut(s) 52
BstBI TTCGAA 1 cut(s) 352
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 7 cut(s) 329, 516, 552, 630, 878, 1119, 1168
BstDSI CCRYGG 1 cut(s) 805
BstF5I GGATG 2 cut(s) 768, 1108
BstHHI GCGC 1 cut(s) 94
BstMAI GTCTC 2 cut(s) 44, 171
BstMCI CGRYCG 1 cut(s) 700
BstMWI GCNNNNNNNGC 4 cut(s) 218, 319, 380, 810
BstNSI RCATGY 1 cut(s) 1200
BstSFI CTRYAG 1 cut(s) 675
BstSLI GKGCMC 1 cut(s) 1113
BstV1I GCAGC 7 cut(s) 196, 592, 661, 689, 840, 913, 1111
BstX2I RGATCY 4 cut(s) 538, 984, 1078, 1171
BstYI RGATCY 4 cut(s) 538, 984, 1078, 1171
Bsu36I CCTNAGG 1 cut(s) 552
BsuI GTATCC 3 cut(s) 714, 785, 989
BsuRI GGCC 1 cut(s) 591
BtgI CCRYGG 1 cut(s) 805
BtsCI GGATG 2 cut(s) 768, 1108
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 5 cut(s) 109, 280, 729, 1116, 1162
BveI ACCTGC 1 cut(s) 1055
Cac8I GCNNGC 1 cut(s) 73
CfoI GCGC 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 1 cut(s) 734
Csp6I GTAC 3 cut(s) 492, 627, 694
CspAI ACCGGT 1 cut(s) 194
CviAII CATG 7 cut(s) 223, 420, 773, 832, 1001, 1016, 1197
CviQI GTAC 3 cut(s) 492, 627, 694
DdeI CTNAG 7 cut(s) 329, 516, 552, 630, 878, 1119, 1168
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
Eco24I GRGCYC 1 cut(s) 785
Eco47I GGWCC 1 cut(s) 734
Eco81I CCTNAGG 1 cut(s) 552
EcoRI GAATTC 1 cut(s) 184
EcoT22I ATGCAT 1 cut(s) 1198
EcoT38I GRGCYC 1 cut(s) 785
FaeI CATG 7 cut(s) 226, 423, 776, 835, 1004, 1019, 1200
FatI CATG 7 cut(s) 222, 419, 772, 831, 1000, 1015, 1196
FauNDI CATATG 2 cut(s) 19, 910
FbaI TGATCA 2 cut(s) 99, 475
Fnu4HI GCNGC 8 cut(s) 210, 407, 606, 675, 678, 854, 927, 1125
FokI GGATG 2 cut(s) 755, 1115
FriOI GRGCYC 1 cut(s) 785
Fsp4HI GCNGC 8 cut(s) 210, 407, 606, 675, 678, 854, 927, 1125
FspBI CTAG 4 cut(s) 95, 546, 602, 641
GlaI GCGC 1 cut(s) 93
GluI GCNGC 8 cut(s) 210, 407, 606, 675, 678, 854, 927, 1125
GsaI CCCAGC 3 cut(s) 267, 317, 1102
GsuI CTGGAG 1 cut(s) 408
HaeIII GGCC 1 cut(s) 591
HapII CCGG 3 cut(s) 195, 653, 789
HhaI GCGC 1 cut(s) 94
Hin1II CATG 7 cut(s) 226, 423, 776, 835, 1004, 1019, 1200
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 1 cut(s) 959
HinfI GANTC 4 cut(s) 179, 344, 416, 439
HpaI GTTAAC 1 cut(s) 664
HpaII CCGG 3 cut(s) 195, 653, 789
HphI GGTGA 3 cut(s) 123, 125, 222
Hpy166II GTNNAC 2 cut(s) 664, 1111
Hpy188I TCNGA 5 cut(s) 190, 618, 686, 780, 1171
Hpy188III TCNNGA 4 cut(s) 387, 452, 653, 718
Hpy8I GTNNAC 2 cut(s) 664, 1111
HpyAV CCTTC 3 cut(s) 144, 877, 1092
HpyCH4III ACNGT 3 cut(s) 371, 809, 843
HpyF10VI GCNNNNNNNGC 4 cut(s) 218, 319, 380, 810
HpyF3I CTNAG 7 cut(s) 329, 516, 552, 630, 878, 1119, 1168
Hsp92II CATG 7 cut(s) 226, 423, 776, 835, 1004, 1019, 1200
HspAI GCGC 1 cut(s) 92
Kpn2I TCCGGA 1 cut(s) 652
Ksp22I TGATCA 2 cut(s) 99, 475
KspAI GTTAAC 1 cut(s) 664
LmnI GCTCC 1 cut(s) 392
Lsp1109I GCAGC 7 cut(s) 196, 592, 661, 689, 840, 913, 1111
LweI GCATC 1 cut(s) 112
MaeI CTAG 4 cut(s) 95, 546, 602, 641
MaeIII GTNAC 3 cut(s) 24, 228, 754
MboII GAAGA 7 cut(s) 69, 128, 131, 241, 994, 1016, 1088
MflI RGATCY 4 cut(s) 538, 984, 1078, 1171
MhlI GDGCHC 2 cut(s) 785, 1113
MluCI AATT 6 cut(s) 184, 348, 358, 376, 648, 1149
MlyI GAGTC 4 cut(s) 173, 338, 425, 448
MmeI TCCRAC 1 cut(s) 898
MnlI CCTC 7 cut(s) 104, 261, 383, 459, 547, 695, 1114
Mph1103I ATGCAT 1 cut(s) 1198
MroI TCCGGA 1 cut(s) 652
MseI TTAA 2 cut(s) 663, 1210
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 3 cut(s) 195, 653, 789
Mva1269I GAATGC 2 cut(s) 212, 713
MwoI GCNNNNNNNGC 4 cut(s) 218, 319, 380, 810
NdeI CATATG 2 cut(s) 19, 910
NlaIII CATG 7 cut(s) 226, 423, 776, 835, 1004, 1019, 1200
NmeAIII GCCGAG 1 cut(s) 331
NmuCI GTSAC 2 cut(s) 228, 754
NsiI ATGCAT 1 cut(s) 1198
NspI RCATGY 1 cut(s) 1200
NspV TTCGAA 1 cut(s) 352
PctI GAATGC 2 cut(s) 212, 713
PinAI ACCGGT 1 cut(s) 194
PkrI GCNGC 8 cut(s) 211, 408, 607, 676, 679, 855, 928, 1126
PleI GAGTC 4 cut(s) 173, 338, 424, 447
PpsI GAGTC 4 cut(s) 173, 338, 424, 447
PspFI CCCAGC 3 cut(s) 263, 313, 1098
PspPI GGNCC 1 cut(s) 734
PsrI GAACNNNNNNTAC 2 cut(s) 464, 496
PstI CTGCAG 1 cut(s) 679
PsuI RGATCY 4 cut(s) 538, 984, 1078, 1171
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 3 cut(s) 493, 628, 695
RsaNI GTAC 3 cut(s) 492, 627, 694
SaqAI TTAA 2 cut(s) 663, 1210
SatI GCNGC 8 cut(s) 210, 407, 606, 675, 678, 854, 927, 1125
Sau96I GGNCC 1 cut(s) 734
ScaI AGTACT 1 cut(s) 628
SchI GAGTC 4 cut(s) 173, 338, 425, 448
SduI GDGCHC 2 cut(s) 785, 1113
SfaNI GCATC 1 cut(s) 112
SfcI CTRYAG 1 cut(s) 675
SfuI TTCGAA 1 cut(s) 352
SinI GGWCC 1 cut(s) 734
Sse9I AATT 6 cut(s) 184, 348, 358, 376, 648, 1149
SsiI CCGC 1 cut(s) 406
SspMI CTAG 4 cut(s) 95, 546, 602, 641
TaaI ACNGT 3 cut(s) 371, 809, 843
TaqI TCGA 5 cut(s) 182, 352, 364, 528, 972
TaqII GACCGA 1 cut(s) 714
TasI AATT 6 cut(s) 184, 348, 358, 376, 648, 1149
TatI WGTACW 1 cut(s) 626
TauI GCSGC 1 cut(s) 409
Tru1I TTAA 2 cut(s) 663, 1210
Tru9I TTAA 2 cut(s) 663, 1210
TscAI CASTG 5 cut(s) 109, 280, 736, 1123, 1162
TseFI GTSAC 2 cut(s) 228, 754
TseI GCWGC 7 cut(s) 209, 605, 674, 677, 853, 926, 1124
Tsp45I GTSAC 2 cut(s) 228, 754
TspDTI ATGAA 6 cut(s) 117, 289, 324, 1017, 1068, 1152
TspRI CASTG 5 cut(s) 109, 280, 736, 1123, 1162
VneI GTGCAC 1 cut(s) 1109
VpaK11BI GGWCC 1 cut(s) 734
XapI RAATTY 2 cut(s) 184, 648
XceI RCATGY 1 cut(s) 1200
XcmI CCANNNNNNNNNTGG 1 cut(s) 1022
XspI CTAG 4 cut(s) 95, 546, 602, 641
ZrmI AGTACT 1 cut(s) 628
Zsp2I ATGCAT 1 cut(s) 1198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.