Rroxscaffold_2G00078940

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002827
Physical Location & Seq
Forward (+)
2056032 .. 2057952
1921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00078940.1

Sequence Viewer

Length: 1164 bp
ATGGTGGGAATGGAGATTTGGAAACAACTACGGCTACTGTCTTCTGGGCTGTTCTTGGCTTTGTGCGTTCTGGGTGTGAGCGGTGGTGCGTTACCATCTTGTTCTTTTCCGGCGATCTACAACTTCGGCGACTCGAACTCCGACACTGGCGGGATCTCAGCTGCATTTGAACCGATTCGGGCACCCTACGGCGAGGGGTTCTTTCGTAAACCAGCTGGAAGGGACTCCGATGGTCGTCTCATCATAGACTTCATAGCTGAGCACCTAAGGTTGCCGTACTTGAGTGCATACTTGAACTCGCTGGGAACAAATTACGGGCATGGTGCAAATTTTGCCACCGGAGGATCAACCATTAGGAGGCCGAATGAGACCATTTTCCAGTATGGCATAAGCCCCTTCTCGCTTGATATGCAGACGGCTCAATTCCTGCAGTTCAAAGCTCGAACAGCTGATCTATTTCGACAAGCCAAGAATCCTTCTGATAAAAGCAAACTTCCAAATCCTCAAGACTTTGCAAAGGCACTGTATACGTTTGATATTGGGCAAAATGATCTCTCTGTTGGTTTTCGAAAGCTAAGCTTTGATCAGCTTCGTGCAGAAATTCCAGACATTGTAAACCAGCTGGCCACAGCAGTCCGAGTGACGTATATACGAACAAGGGGGAGGGCATTTTGGATACACAACACTGGTCCGATTGGTTGCTTGCCAGTGAATCTGTTCTACAATCTTAATCCCGCACCGGGTTATGTCGACGAACATGGCTGTGTCAAGGGCCAAAACGACATGGCCATAGAATTCAACAGGCAGCTTAAGGACAGAGTCATCAAACTAAGAGCTGAGCTCCCACAAGCTGCAATTACATATGTAGATGCCTATGCTGCTAAGTATGGACTCATCAGCAATGCAAAGAATGAAGGGTTTGTGGATCCAATGAAGGTTTGCTGTGGGTATCATGTGCGATATGACCATGTGTGGTGTGGAAACAAAGCAACTGTAAATGGAAGTCTAGTATATGGTGCTTCATGTGGAAATCCTTCATCTTCTATAAGCTGGGATGGAGTTCACTACACTCAGGCTGCCAATCAGTGGGTTGCTAATCATATACTCAATGGCGCCTTGTCCAACCCACCGATTCCCATTACTCAAGCTTGTCATAGGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

387

Amino Acids

42.49

Weight (kDa)

8.76

Isoelectric Point (pI)

29.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 39 - 368 1.7e-41 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 181, 1112
AccB7I CCANNNNNTGG 1 cut(s) 1086
AccBSI CCGCTC 1 cut(s) 81
AccI GTMKAC 2 cut(s) 527, 750
AciI CCGC 3 cut(s) 81, 150, 735
AclWI GGATC 4 cut(s) 161, 352, 920, 933
AcoI YGGCCR 2 cut(s) 624, 786
AcsI RAATTY 3 cut(s) 328, 600, 794
AcyI GRCGYC 1 cut(s) 1113
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 3 cut(s) 357, 740, 1086
AflII CTTAAG 1 cut(s) 809
AgsI TTSAA 4 cut(s) 170, 295, 436, 799
AjuI GAANNNNNNNTTGG 1 cut(s) 33
Alw21I GWGCWC 2 cut(s) 264, 843
Alw26I GTCTC 2 cut(s) 242, 362
AlwI GGATC 4 cut(s) 161, 352, 920, 933
AoxI GGCC 4 cut(s) 359, 624, 772, 786
ApeKI GCWGC 5 cut(s) 161, 805, 851, 878, 1076
ApoI RAATTY 3 cut(s) 328, 600, 794
Asp700I GAANNNNTTC 3 cut(s) 174, 716, 1033
AspLEI GCGC 1 cut(s) 1115
AspS9I GGNCC 2 cut(s) 689, 772
AsuC2I CCSGG 1 cut(s) 741
AsuII TTCGAA 1 cut(s) 568
AvaII GGWCC 1 cut(s) 689
AxyI CCTNAGG 1 cut(s) 266
BaeGI GKGCMC 1 cut(s) 184
BalI TGGCCA 2 cut(s) 626, 788
BamHI GGATCC 1 cut(s) 925
BanI GGYRCC 2 cut(s) 181, 1112
BanII GRGCYC 1 cut(s) 843
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 2 cut(s) 264, 843
BbvI GCAGC 5 cut(s) 148, 817, 838, 865, 1063
BccI CCATC 3 cut(s) 103, 224, 1049
BceAI ACGGC 4 cut(s) 47, 205, 259, 432
BciVI GTATCC 1 cut(s) 669
BclI TGATCA 1 cut(s) 583
BcnI CCSGG 1 cut(s) 741
BcoDI GTCTC 2 cut(s) 242, 362
BfaI CTAG 2 cut(s) 1007, 1162
BfmI CTRYAG 1 cut(s) 428
BfoI RGCGCY 1 cut(s) 1116
BfrI CTTAAG 1 cut(s) 809
BfuI GTATCC 1 cut(s) 669
BisI GCNGC 5 cut(s) 162, 806, 852, 879, 1077
BlpI GCTNAGC 3 cut(s) 258, 575, 837
BlsI GCNGC 5 cut(s) 163, 807, 853, 880, 1078
Bme1390I CCNGG 1 cut(s) 741
Bme18I GGWCC 1 cut(s) 689
BmgT120I GGNCC 2 cut(s) 689, 772
BmiI GGNNCC 3 cut(s) 183, 927, 1114
BmrFI CCNGG 1 cut(s) 741
BmsI GCATC 1 cut(s) 859
BpiI GAAGAC 1 cut(s) 33
BplI GAGNNNNNCTC 2 cut(s) 825, 857
Bpu1102I GCTNAGC 3 cut(s) 258, 575, 837
Bpu14I TTCGAA 1 cut(s) 568
BpuEI CTTGAG 3 cut(s) 301, 489, 1128
BpuMI CCSGG 1 cut(s) 741
BsaHI GRCGYC 1 cut(s) 1113
BsaI GGTCTC 1 cut(s) 362
BsaWI WCCGGW 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 3 cut(s) 357, 740, 1086
Bse1I ACTGG 4 cut(s) 151, 379, 691, 707
Bse21I CCTNAGG 1 cut(s) 266
Bse3DI GCAATG 1 cut(s) 907
BseGI GGATG 1 cut(s) 1060
BseLI CCNNNNNNNGG 3 cut(s) 357, 740, 1086
BseMI GCAATG 1 cut(s) 907
BseMII CTCAG 4 cut(s) 171, 249, 828, 1085
BseNI ACTGG 4 cut(s) 151, 379, 691, 707
BseSI GKGCMC 1 cut(s) 184
BseXI GCAGC 5 cut(s) 148, 817, 838, 865, 1063
BseYI CCCAGC 2 cut(s) 301, 1050
BsgI GTGCAG 1 cut(s) 615
BshFI GGCC 4 cut(s) 361, 626, 774, 788
BshNI GGYRCC 2 cut(s) 181, 1112
BsiHKAI GWGCWC 2 cut(s) 264, 843
BsiSI CCGG 3 cut(s) 110, 339, 740
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 3 cut(s) 357, 740, 1086
BsmAI GTCTC 2 cut(s) 242, 362
BsmBI CGTCTC 1 cut(s) 242
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 4 cut(s) 361, 626, 774, 788
Bso31I GGTCTC 1 cut(s) 362
Bsp119I TTCGAA 1 cut(s) 568
Bsp1286I GDGCHC 3 cut(s) 184, 264, 843
Bsp143I GATC 7 cut(s) 114, 153, 344, 451, 550, 583, 925
Bsp1720I GCTNAGC 3 cut(s) 258, 575, 837
BspACI CCGC 3 cut(s) 81, 150, 735
BspANI GGCC 4 cut(s) 361, 626, 774, 788
BspCNI CTCAG 4 cut(s) 170, 250, 829, 1084
BspLI GGNNCC 3 cut(s) 183, 927, 1114
BspMAI CTGCAG 1 cut(s) 432
BspPI GGATC 4 cut(s) 161, 352, 920, 933
BspT104I TTCGAA 1 cut(s) 568
BspT107I GGYRCC 2 cut(s) 181, 1112
BspTI CTTAAG 1 cut(s) 809
BspTNI GGTCTC 1 cut(s) 362
BsrBI CCGCTC 1 cut(s) 81
BsrDI GCAATG 1 cut(s) 907
BsrI ACTGG 4 cut(s) 151, 379, 691, 707
BssMI GATC 7 cut(s) 114, 153, 344, 451, 550, 583, 925
BssNAI GTATAC 1 cut(s) 528
BssNI GRCGYC 1 cut(s) 1113
Bst1107I GTATAC 1 cut(s) 528
Bst4CI ACNGT 3 cut(s) 39, 525, 994
BstACI GRCGYC 1 cut(s) 1113
BstAFI CTTAAG 1 cut(s) 809
BstAPI GCANNNNNTGC 1 cut(s) 332
BstBI TTCGAA 1 cut(s) 568
BstC8I GCNNGC 2 cut(s) 624, 704
BstDEI CTNAG 8 cut(s) 157, 258, 266, 575, 830, 837, 882, 1071
BstF5I GGATG 1 cut(s) 1060
BstH2I RGCGCY 1 cut(s) 1116
BstHHI GCGC 1 cut(s) 1115
BstKTI GATC 7 cut(s) 117, 156, 347, 454, 553, 586, 928
BstMAI GTCTC 2 cut(s) 242, 362
BstMBI GATC 7 cut(s) 114, 153, 344, 451, 550, 583, 925
BstMWI GCNNNNNNNGC 4 cut(s) 332, 409, 446, 878
BstSCI CCNGG 1 cut(s) 739
BstSFI CTRYAG 1 cut(s) 428
BstSLI GKGCMC 1 cut(s) 184
BstV1I GCAGC 5 cut(s) 148, 817, 838, 865, 1063
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 2 cut(s) 153, 925
BstYI RGATCY 2 cut(s) 153, 925
BstZ17I GTATAC 1 cut(s) 528
Bsu36I CCTNAGG 1 cut(s) 266
BsuI GTATCC 1 cut(s) 669
BsuRI GGCC 4 cut(s) 361, 626, 774, 788
BtsCI GGATG 1 cut(s) 1060
BtsIMutI CAGTG 5 cut(s) 144, 521, 684, 714, 1091
Cac8I GCNNGC 2 cut(s) 624, 704
CfoI GCGC 1 cut(s) 1115
Cfr13I GGNCC 2 cut(s) 689, 772
Csp6I GTAC 1 cut(s) 277
CviAII CATG 6 cut(s) 320, 758, 784, 953, 968, 1023
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 8 cut(s) 157, 258, 266, 575, 830, 837, 882, 1071
DinI GGCGCC 1 cut(s) 1114
DpnI GATC 7 cut(s) 116, 155, 346, 453, 552, 585, 927
DpnII GATC 7 cut(s) 114, 153, 344, 451, 550, 583, 925
EaeI YGGCCR 2 cut(s) 624, 786
Ecl136II GAGCTC 1 cut(s) 841
Eco24I GRGCYC 1 cut(s) 843
Eco31I GGTCTC 1 cut(s) 362
Eco47I GGWCC 1 cut(s) 689
Eco53kI GAGCTC 1 cut(s) 841
Eco81I CCTNAGG 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 841
EcoRI GAATTC 1 cut(s) 794
EcoT38I GRGCYC 1 cut(s) 843
EgeI GGCGCC 1 cut(s) 1114
EheI GGCGCC 1 cut(s) 1114
Esp3I CGTCTC 1 cut(s) 242
FaeI CATG 6 cut(s) 323, 761, 787, 956, 971, 1026
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FaqI GGGAC 1 cut(s) 236
FatI CATG 6 cut(s) 319, 757, 783, 952, 967, 1022
FauI CCCGC 2 cut(s) 143, 742
FauNDI CATATG 1 cut(s) 862
FbaI TGATCA 1 cut(s) 583
FblI GTMKAC 2 cut(s) 527, 750
Fnu4HI GCNGC 5 cut(s) 162, 806, 852, 879, 1077
FokI GGATG 1 cut(s) 1067
FriOI GRGCYC 1 cut(s) 843
Fsp4HI GCNGC 5 cut(s) 162, 806, 852, 879, 1077
FspBI CTAG 2 cut(s) 1007, 1162
GlaI GCGC 1 cut(s) 1114
GluI GCNGC 5 cut(s) 162, 806, 852, 879, 1077
GsaI CCCAGC 2 cut(s) 305, 1054
HaeII RGCGCY 1 cut(s) 1116
HaeIII GGCC 4 cut(s) 361, 626, 774, 788
HapII CCGG 3 cut(s) 110, 339, 740
HhaI GCGC 1 cut(s) 1115
Hin1I GRCGYC 1 cut(s) 1113
Hin1II CATG 6 cut(s) 323, 761, 787, 956, 971, 1026
Hin6I GCGC 1 cut(s) 1113
HinP1I GCGC 1 cut(s) 1113
HincII GTYRAC 1 cut(s) 751
HindII GTYRAC 1 cut(s) 751
HindIII AAGCTT 2 cut(s) 577, 1146
HinfI GANTC 8 cut(s) 131, 175, 224, 472, 712, 819, 891, 1132
HpaII CCGG 3 cut(s) 110, 339, 740
Hpy166II GTNNAC 5 cut(s) 209, 528, 616, 751, 1063
Hpy188I TCNGA 5 cut(s) 142, 229, 481, 638, 693
Hpy188III TCNNGA 2 cut(s) 506, 605
Hpy8I GTNNAC 5 cut(s) 209, 528, 616, 751, 1063
Hpy99I CGWCG 1 cut(s) 755
HpyAV CCTTC 6 cut(s) 213, 406, 486, 908, 928, 1044
HpyCH4III ACNGT 3 cut(s) 39, 525, 994
HpyCH4IV ACGT 2 cut(s) 530, 644
HpyCH4V TGCA 9 cut(s) 164, 287, 326, 412, 430, 515, 596, 854, 905
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 409, 446, 878
HpyF3I CTNAG 8 cut(s) 157, 258, 266, 575, 830, 837, 882, 1071
HpySE526I ACGT 2 cut(s) 530, 644
Hsp92I GRCGYC 1 cut(s) 1113
Hsp92II CATG 6 cut(s) 323, 761, 787, 956, 971, 1026
HspAI GCGC 1 cut(s) 1113
KasI GGCGCC 1 cut(s) 1112
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 7 cut(s) 114, 153, 344, 451, 550, 583, 925
LmnI GCTCC 2 cut(s) 846, 1158
Lsp1109I GCAGC 5 cut(s) 148, 817, 838, 865, 1063
LweI GCATC 1 cut(s) 859
MaeI CTAG 2 cut(s) 1007, 1162
MaeII ACGT 2 cut(s) 530, 644
MaeIII GTNAC 2 cut(s) 90, 640
MalI GATC 7 cut(s) 116, 155, 346, 453, 552, 585, 927
MbiI CCGCTC 1 cut(s) 81
MboI GATC 7 cut(s) 114, 153, 344, 451, 550, 583, 925
MboII GAAGA 2 cut(s) 33, 1032
MflI RGATCY 2 cut(s) 153, 925
MhlI GDGCHC 3 cut(s) 184, 264, 843
MlsI TGGCCA 2 cut(s) 626, 788
MluCI AATT 6 cut(s) 310, 328, 422, 600, 794, 855
MluNI TGGCCA 2 cut(s) 626, 788
Mly113I GGCGCC 1 cut(s) 1113
MlyI GAGTC 4 cut(s) 125, 218, 828, 885
MmeI TCCRAC 2 cut(s) 165, 1146
MnlI CCTC 5 cut(s) 187, 335, 351, 513, 657
Mox20I TGGCCA 2 cut(s) 626, 788
MroXI GAANNNNTTC 3 cut(s) 174, 716, 1033
MscI TGGCCA 2 cut(s) 626, 788
MseI TTAA 2 cut(s) 729, 810
MslI CAYNNNNRTG 1 cut(s) 762
Msp20I TGGCCA 2 cut(s) 626, 788
MspA1I CMGCKG 4 cut(s) 161, 215, 449, 622
MspCI CTTAAG 1 cut(s) 809
MspI CCGG 3 cut(s) 110, 339, 740
MspR9I CCNGG 1 cut(s) 741
MwoI GCNNNNNNNGC 4 cut(s) 332, 409, 446, 878
NarI GGCGCC 1 cut(s) 1113
NciI CCSGG 1 cut(s) 741
NdeI CATATG 1 cut(s) 862
NdeII GATC 7 cut(s) 114, 153, 344, 451, 550, 583, 925
NlaIII CATG 6 cut(s) 323, 761, 787, 956, 971, 1026
NlaIV GGNNCC 3 cut(s) 183, 927, 1114
NmuCI GTSAC 1 cut(s) 640
NspV TTCGAA 1 cut(s) 568
PdmI GAANNNNTTC 3 cut(s) 174, 716, 1033
PfeI GAWTC 4 cut(s) 175, 472, 712, 1132
PflFI GACNNNGTC 1 cut(s) 818
PflMI CCANNNNNTGG 1 cut(s) 1086
PkrI GCNGC 5 cut(s) 163, 807, 853, 880, 1078
PleI GAGTC 4 cut(s) 125, 218, 827, 885
PluTI GGCGCC 1 cut(s) 1116
PpsI GAGTC 4 cut(s) 125, 218, 827, 885
Psp124BI GAGCTC 1 cut(s) 843
PspFI CCCAGC 2 cut(s) 301, 1050
PspN4I GGNNCC 3 cut(s) 183, 927, 1114
PspPI GGNCC 2 cut(s) 689, 772
PstI CTGCAG 1 cut(s) 432
PsuI RGATCY 2 cut(s) 153, 925
PsyI GACNNNGTC 1 cut(s) 818
PvuII CAGCTG 4 cut(s) 161, 215, 449, 622
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
RseI CAYNNNNRTG 1 cut(s) 762
SacI GAGCTC 1 cut(s) 843
SalI GTCGAC 1 cut(s) 749
SaqAI TTAA 2 cut(s) 729, 810
SatI GCNGC 5 cut(s) 162, 806, 852, 879, 1077
Sau3AI GATC 7 cut(s) 114, 153, 344, 451, 550, 583, 925
Sau96I GGNCC 2 cut(s) 689, 772
SchI GAGTC 4 cut(s) 125, 218, 828, 885
ScrFI CCNGG 1 cut(s) 741
SduI GDGCHC 3 cut(s) 184, 264, 843
SfaNI GCATC 1 cut(s) 859
SfcI CTRYAG 1 cut(s) 428
SfoI GGCGCC 1 cut(s) 1114
SfuI TTCGAA 1 cut(s) 568
SinI GGWCC 1 cut(s) 689
SmiMI CAYNNNNRTG 1 cut(s) 762
SmlI CTYRAG 4 cut(s) 280, 504, 809, 1143
SmoI CTYRAG 4 cut(s) 280, 504, 809, 1143
Sse9I AATT 6 cut(s) 310, 328, 422, 600, 794, 855
SsiI CCGC 3 cut(s) 81, 150, 735
SspDI GGCGCC 1 cut(s) 1112
SspMI CTAG 2 cut(s) 1007, 1162
SstI GAGCTC 1 cut(s) 843
StyD4I CCNGG 1 cut(s) 739
TaaI ACNGT 3 cut(s) 39, 525, 994
TaiI ACGT 2 cut(s) 533, 647
TaqI TCGA 5 cut(s) 134, 442, 460, 568, 750
TasI AATT 6 cut(s) 310, 328, 422, 600, 794, 855
TfiI GAWTC 4 cut(s) 175, 472, 712, 1132
Tru1I TTAA 2 cut(s) 729, 810
Tru9I TTAA 2 cut(s) 729, 810
TscAI CASTG 5 cut(s) 151, 528, 691, 714, 1091
TseFI GTSAC 1 cut(s) 640
TseI GCWGC 5 cut(s) 161, 805, 851, 878, 1076
Tsp45I GTSAC 1 cut(s) 640
TspDTI ATGAA 5 cut(s) 241, 927, 947, 1011, 1026
TspRI CASTG 5 cut(s) 151, 528, 691, 714, 1091
Tth111I GACNNNGTC 1 cut(s) 818
Van91I CCANNNNNTGG 1 cut(s) 1086
Vha464I CTTAAG 1 cut(s) 809
VpaK11BI GGWCC 1 cut(s) 689
XapI RAATTY 3 cut(s) 328, 600, 794
XcmI CCANNNNNNNNNTGG 1 cut(s) 974
XmiI GTMKAC 2 cut(s) 527, 750
XmnI GAANNNNTTC 3 cut(s) 174, 716, 1033
XspI CTAG 2 cut(s) 1007, 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.