FvH4_6g51890

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002827
Physical Location & Seq
Reverse (-)
38395309 .. 38397662
2354 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g51890.t2

Sequence Viewer

Length: 1161 bp
ATGCTGGGAATGGAGATTTGGAAGCAGCTAGTGCTACTGCCTTGTGGGTTGTTCTTGACTTTGTGGGTTCTGGGTGTGAGCGGAGCTACGTTACCATCTTGTTCTTTTCCGGCAATCTACAACTTCGGCGACTCGAACTCCGACACCGGCGGGATCTCAGCTGCATTTGAGCCGATTCGGACACCCTACGGTGAAGGGTTCTTTCGTAAACCAGCTGGAAGGGACTCCGATGGTCGTCTCATCATAGACTTCATAGCTGAGGGCCTAAAGTTGCCATACTTGAGTGCATACTTGAACTCGCTGGGAACAAATTACAAGCATGGTGCAAATTTTGCTACCGGAGGATCAACAATTAGAAGGCCGAATGAGACCATTTTCGAGAATGGTATAAGTCCCTTCTCGCTTGATATGCAGACGGCTCAGTTCCTGCAGTTCAAATCTAGAACGGCTGATCTGTTTCGACAAGCTAAGAATCCCTATGAGAGAAGCAGACTTCCAAATCCTCAAGACTTTGCAAAGGCACTGTATACGTTTGATATTGGGCAGAATGATCTCTCTGCTGGTTTTCGAAAGCTGAGCTTTGATCAGCTTCGTGCACAGATTCCAGACATTGTAAACCAGCTGGCCACAGCAGTCCGACGTATATACGAACAAGGGGGCAGGGCATTTTGGATACACAACACTGGTCCGATTGGTTGCTTGCCAATCAATCTGTTCTACAATCTTAATCCTGCACCGGGTTATGTCGATGAACATGGCTGTGTCAAGGGCCAAAACGACATGGCCATAGAATTCAACAGGCAGCTCAAGGACAGAGTCATCAAACTAAGAGCTGAGCTCCCACAAGCTGCAATTACATATGTGGATGCCTATGCTGCAAAGTATGGACTCATCAGCAATGCAAAGAATGAAGGGTTTGTGGATCCAATGAAGGTTTGTTGTGGGTATCATGTGCGATATGACCATGTGTGGTGTGGAAACAAAGCAATTGTAAATGGGAGAGAAGTGTATGGTGCTTCTTGTGGAAATGCTTCATCTGCTATAAGCTGGGATGGAGTTCACTACACTCAGGCTGCCAATCAGTGGGTGGCCAATCATATACTCAATGGCGCCTTGTCCAACCCACCGATTCCCATTACTCAGGCTTGTCATAGGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

387

Amino Acids

42.64

Weight (kDa)

8.48

Isoelectric Point (pI)

30.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 13 - 286 6e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1109
AccB7I CCANNNNNTGG 1 cut(s) 1083
AccBSI CCGCTC 1 cut(s) 81
AccI GTMKAC 1 cut(s) 527
AciI CCGC 2 cut(s) 81, 150
AclWI GGATC 4 cut(s) 161, 352, 917, 930
AcoI YGGCCR 3 cut(s) 624, 783, 1089
AcsI RAATTY 2 cut(s) 328, 791
AcyI GRCGYC 1 cut(s) 1110
AfiI CCNNNNNNNGG 2 cut(s) 737, 1083
AgsI TTSAA 3 cut(s) 295, 436, 796
AjuI GAANNNNNNNTTGG 1 cut(s) 33
Alw21I GWGCWC 2 cut(s) 598, 840
Alw26I GTCTC 2 cut(s) 242, 362
Alw44I GTGCAC 1 cut(s) 594
AlwI GGATC 4 cut(s) 161, 352, 917, 930
AlwNI CAGNNNCTG 1 cut(s) 427
AoxI GGCC 6 cut(s) 262, 359, 624, 769, 783, 1089
ApaLI GTGCAC 1 cut(s) 594
ApeKI GCWGC 6 cut(s) 25, 161, 802, 848, 875, 1073
ApoI RAATTY 2 cut(s) 328, 791
Asp700I GAANNNNTTC 1 cut(s) 1030
AspLEI GCGC 1 cut(s) 1112
AspS9I GGNCC 3 cut(s) 262, 686, 769
AsuC2I CCSGG 1 cut(s) 738
AsuHPI GGTGA 1 cut(s) 203
AsuII TTCGAA 1 cut(s) 568
AvaII GGWCC 1 cut(s) 686
BaeGI GKGCMC 1 cut(s) 598
BalI TGGCCA 3 cut(s) 626, 785, 1091
BamHI GGATCC 1 cut(s) 922
BanI GGYRCC 1 cut(s) 1109
BanII GRGCYC 1 cut(s) 840
Bbv12I GWGCWC 2 cut(s) 598, 840
BbvCI CCTCAGC 1 cut(s) 258
BbvI GCAGC 6 cut(s) 37, 148, 814, 835, 862, 1060
BccI CCATC 3 cut(s) 103, 224, 1046
BceAI ACGGC 2 cut(s) 432, 462
BciVI GTATCC 1 cut(s) 666
BclI TGATCA 1 cut(s) 583
BcnI CCSGG 1 cut(s) 738
BcoDI GTCTC 2 cut(s) 242, 362
BfaI CTAG 2 cut(s) 29, 441
BfmI CTRYAG 1 cut(s) 428
BfoI RGCGCY 1 cut(s) 1113
BfuI GTATCC 1 cut(s) 666
BisI GCNGC 6 cut(s) 26, 162, 803, 849, 876, 1074
BlpI GCTNAGC 2 cut(s) 575, 834
BlsI GCNGC 6 cut(s) 27, 163, 804, 850, 877, 1075
Bme1390I CCNGG 1 cut(s) 738
Bme18I GGWCC 1 cut(s) 686
BmgT120I GGNCC 3 cut(s) 262, 686, 769
BmiI GGNNCC 2 cut(s) 924, 1111
BmrFI CCNGG 1 cut(s) 738
BmsI GCATC 1 cut(s) 856
BplI GAGNNNNNCTC 2 cut(s) 822, 854
Bpu10I CCTNAGC 1 cut(s) 258
Bpu1102I GCTNAGC 2 cut(s) 575, 834
Bpu14I TTCGAA 1 cut(s) 568
BpuEI CTTGAG 3 cut(s) 301, 489, 791
BpuMI CCSGG 1 cut(s) 738
BsaHI GRCGYC 1 cut(s) 1110
BsaI GGTCTC 1 cut(s) 362
BsaWI WCCGGW 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 2 cut(s) 737, 1083
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 1 cut(s) 688
Bse3DI GCAATG 1 cut(s) 904
BseGI GGATG 2 cut(s) 871, 1057
BseLI CCNNNNNNNGG 2 cut(s) 737, 1083
BseMI GCAATG 1 cut(s) 904
BseMII CTCAG 7 cut(s) 171, 249, 434, 566, 825, 1082, 1154
BseNI ACTGG 1 cut(s) 688
BseSI GKGCMC 1 cut(s) 598
BseXI GCAGC 6 cut(s) 37, 148, 814, 835, 862, 1060
BseYI CCCAGC 3 cut(s) 4, 301, 1047
BsgI GTGCAG 1 cut(s) 717
BshFI GGCC 6 cut(s) 264, 361, 626, 771, 785, 1091
BshNI GGYRCC 1 cut(s) 1109
BsiHKAI GWGCWC 2 cut(s) 598, 840
BsiSI CCGG 4 cut(s) 110, 147, 339, 737
BslFI GGGAC 2 cut(s) 236, 378
BslI CCNNNNNNNGG 2 cut(s) 737, 1083
BsmAI GTCTC 2 cut(s) 242, 362
BsmBI CGTCTC 1 cut(s) 242
BsmFI GGGAC 2 cut(s) 236, 378
BsnI GGCC 6 cut(s) 264, 361, 626, 771, 785, 1091
Bso31I GGTCTC 1 cut(s) 362
Bsp119I TTCGAA 1 cut(s) 568
Bsp1286I GDGCHC 2 cut(s) 598, 840
Bsp143I GATC 6 cut(s) 153, 344, 451, 550, 583, 922
Bsp1720I GCTNAGC 2 cut(s) 575, 834
BspACI CCGC 2 cut(s) 81, 150
BspANI GGCC 6 cut(s) 264, 361, 626, 771, 785, 1091
BspCNI CTCAG 7 cut(s) 170, 250, 433, 567, 826, 1081, 1153
BspLI GGNNCC 2 cut(s) 924, 1111
BspMAI CTGCAG 1 cut(s) 432
BspPI GGATC 4 cut(s) 161, 352, 917, 930
BspT104I TTCGAA 1 cut(s) 568
BspT107I GGYRCC 1 cut(s) 1109
BspTNI GGTCTC 1 cut(s) 362
BsrBI CCGCTC 1 cut(s) 81
BsrDI GCAATG 1 cut(s) 904
BsrFI RCCGGY 1 cut(s) 146
BsrI ACTGG 1 cut(s) 688
BssAI RCCGGY 1 cut(s) 146
BssMI GATC 6 cut(s) 153, 344, 451, 550, 583, 922
BssNAI GTATAC 1 cut(s) 528
BssNI GRCGYC 1 cut(s) 1110
Bst1107I GTATAC 1 cut(s) 528
Bst4CI ACNGT 2 cut(s) 191, 525
BstACI GRCGYC 1 cut(s) 1110
BstAPI GCANNNNNTGC 2 cut(s) 31, 332
BstBI TTCGAA 1 cut(s) 568
BstC8I GCNNGC 2 cut(s) 624, 701
BstDEI CTNAG 9 cut(s) 157, 258, 420, 468, 575, 827, 834, 1068, 1140
BstF5I GGATG 2 cut(s) 871, 1057
BstH2I RGCGCY 1 cut(s) 1113
BstHHI GCGC 1 cut(s) 1112
BstKTI GATC 6 cut(s) 156, 347, 454, 553, 586, 925
BstMAI GTCTC 2 cut(s) 242, 362
BstMBI GATC 6 cut(s) 153, 344, 451, 550, 583, 922
BstMWI GCNNNNNNNGC 5 cut(s) 31, 332, 409, 875, 1037
BstSCI CCNGG 1 cut(s) 736
BstSFI CTRYAG 1 cut(s) 428
BstSLI GKGCMC 1 cut(s) 598
BstV1I GCAGC 6 cut(s) 37, 148, 814, 835, 862, 1060
BstX2I RGATCY 2 cut(s) 153, 922
BstYI RGATCY 2 cut(s) 153, 922
BstZ17I GTATAC 1 cut(s) 528
BsuI GTATCC 1 cut(s) 666
BsuRI GGCC 6 cut(s) 264, 361, 626, 771, 785, 1091
BtsCI GGATG 2 cut(s) 871, 1057
BtsIMutI CAGTG 3 cut(s) 521, 681, 1088
Cac8I GCNNGC 2 cut(s) 624, 701
CaiI CAGNNNCTG 1 cut(s) 427
CfoI GCGC 1 cut(s) 1112
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 3 cut(s) 262, 686, 769
CviAII CATG 5 cut(s) 320, 755, 781, 950, 965
DdeI CTNAG 9 cut(s) 157, 258, 420, 468, 575, 827, 834, 1068, 1140
DinI GGCGCC 1 cut(s) 1111
DpnI GATC 6 cut(s) 155, 346, 453, 552, 585, 924
DpnII GATC 6 cut(s) 153, 344, 451, 550, 583, 922
EaeI YGGCCR 3 cut(s) 624, 783, 1089
Ecl136II GAGCTC 1 cut(s) 838
Eco24I GRGCYC 1 cut(s) 840
Eco31I GGTCTC 1 cut(s) 362
Eco47I GGWCC 1 cut(s) 686
Eco53kI GAGCTC 1 cut(s) 838
EcoICRI GAGCTC 1 cut(s) 838
EcoO109I RGGNCCY 1 cut(s) 262
EcoRI GAATTC 1 cut(s) 791
EcoT38I GRGCYC 1 cut(s) 840
EgeI GGCGCC 1 cut(s) 1111
EheI GGCGCC 1 cut(s) 1111
Esp3I CGTCTC 1 cut(s) 242
FaeI CATG 5 cut(s) 323, 758, 784, 953, 968
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FaqI GGGAC 2 cut(s) 236, 378
FatI CATG 5 cut(s) 319, 754, 780, 949, 964
FauI CCCGC 1 cut(s) 143
FauNDI CATATG 1 cut(s) 859
FbaI TGATCA 1 cut(s) 583
FblI GTMKAC 1 cut(s) 527
Fnu4HI GCNGC 6 cut(s) 26, 162, 803, 849, 876, 1074
FokI GGATG 2 cut(s) 878, 1064
FriOI GRGCYC 1 cut(s) 840
Fsp4HI GCNGC 6 cut(s) 26, 162, 803, 849, 876, 1074
FspBI CTAG 2 cut(s) 29, 441
GlaI GCGC 1 cut(s) 1111
GluI GCNGC 6 cut(s) 26, 162, 803, 849, 876, 1074
GsaI CCCAGC 3 cut(s) 8, 305, 1051
HaeII RGCGCY 1 cut(s) 1113
HaeIII GGCC 6 cut(s) 264, 361, 626, 771, 785, 1091
HapII CCGG 4 cut(s) 110, 147, 339, 737
HhaI GCGC 1 cut(s) 1112
Hin1I GRCGYC 1 cut(s) 1110
Hin1II CATG 5 cut(s) 323, 758, 784, 953, 968
Hin6I GCGC 1 cut(s) 1110
HinP1I GCGC 1 cut(s) 1110
HinfI GANTC 8 cut(s) 131, 175, 224, 472, 601, 816, 888, 1129
HpaII CCGG 4 cut(s) 110, 147, 339, 737
HphI GGTGA 1 cut(s) 203
Hpy166II GTNNAC 5 cut(s) 209, 528, 596, 616, 1060
Hpy188I TCNGA 5 cut(s) 142, 180, 229, 638, 690
Hpy188III TCNNGA 5 cut(s) 55, 379, 441, 506, 605
Hpy8I GTNNAC 5 cut(s) 209, 528, 596, 616, 1060
Hpy99I CGWCG 1 cut(s) 642
HpyAV CCTTC 6 cut(s) 188, 213, 351, 406, 905, 925
HpyCH4III ACNGT 2 cut(s) 191, 525
HpyCH4IV ACGT 3 cut(s) 89, 530, 640
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 332, 409, 875, 1037
HpyF3I CTNAG 9 cut(s) 157, 258, 420, 468, 575, 827, 834, 1068, 1140
HpySE526I ACGT 3 cut(s) 89, 530, 640
Hsp92I GRCGYC 1 cut(s) 1110
Hsp92II CATG 5 cut(s) 323, 758, 784, 953, 968
HspAI GCGC 1 cut(s) 1110
KasI GGCGCC 1 cut(s) 1109
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 6 cut(s) 153, 344, 451, 550, 583, 922
LmnI GCTCC 3 cut(s) 83, 843, 1155
Lsp1109I GCAGC 6 cut(s) 37, 148, 814, 835, 862, 1060
LweI GCATC 1 cut(s) 856
MaeI CTAG 2 cut(s) 29, 441
MaeII ACGT 3 cut(s) 89, 530, 640
MaeIII GTNAC 1 cut(s) 90
MalI GATC 6 cut(s) 155, 346, 453, 552, 585, 924
MbiI CCGCTC 1 cut(s) 81
MboI GATC 6 cut(s) 153, 344, 451, 550, 583, 922
MfeI CAATTG 1 cut(s) 987
MflI RGATCY 2 cut(s) 153, 922
MhlI GDGCHC 2 cut(s) 598, 840
MlsI TGGCCA 3 cut(s) 626, 785, 1091
MluCI AATT 6 cut(s) 310, 328, 351, 791, 852, 987
MluNI TGGCCA 3 cut(s) 626, 785, 1091
Mly113I GGCGCC 1 cut(s) 1110
MlyI GAGTC 4 cut(s) 125, 218, 825, 882
MmeI TCCRAC 3 cut(s) 165, 661, 1143
MnlI CCTC 3 cut(s) 253, 335, 513
Mox20I TGGCCA 3 cut(s) 626, 785, 1091
MroXI GAANNNNTTC 1 cut(s) 1030
MscI TGGCCA 3 cut(s) 626, 785, 1091
MseI TTAA 1 cut(s) 726
MslI CAYNNNNRTG 1 cut(s) 759
Msp20I TGGCCA 3 cut(s) 626, 785, 1091
MspA1I CMGCKG 3 cut(s) 161, 215, 622
MspI CCGG 4 cut(s) 110, 147, 339, 737
MspR9I CCNGG 1 cut(s) 738
MunI CAATTG 1 cut(s) 987
MwoI GCNNNNNNNGC 5 cut(s) 31, 332, 409, 875, 1037
NarI GGCGCC 1 cut(s) 1110
NciI CCSGG 1 cut(s) 738
NdeI CATATG 1 cut(s) 859
NdeII GATC 6 cut(s) 153, 344, 451, 550, 583, 922
NlaIII CATG 5 cut(s) 323, 758, 784, 953, 968
NlaIV GGNNCC 2 cut(s) 924, 1111
NspV TTCGAA 1 cut(s) 568
PdmI GAANNNNTTC 1 cut(s) 1030
PfeI GAWTC 4 cut(s) 175, 472, 601, 1129
PflFI GACNNNGTC 1 cut(s) 815
PflMI CCANNNNNTGG 1 cut(s) 1083
PkrI GCNGC 6 cut(s) 27, 163, 804, 850, 877, 1075
PleI GAGTC 4 cut(s) 125, 218, 824, 882
PluTI GGCGCC 1 cut(s) 1113
PpsI GAGTC 4 cut(s) 125, 218, 824, 882
Psp124BI GAGCTC 1 cut(s) 840
PspFI CCCAGC 3 cut(s) 4, 301, 1047
PspN4I GGNNCC 2 cut(s) 924, 1111
PspPI GGNCC 3 cut(s) 262, 686, 769
PstI CTGCAG 1 cut(s) 432
PstNI CAGNNNCTG 1 cut(s) 427
PsuI RGATCY 2 cut(s) 153, 922
PsyI GACNNNGTC 1 cut(s) 815
PvuII CAGCTG 3 cut(s) 161, 215, 622
RseI CAYNNNNRTG 1 cut(s) 759
SacI GAGCTC 1 cut(s) 840
SaqAI TTAA 1 cut(s) 726
SatI GCNGC 6 cut(s) 26, 162, 803, 849, 876, 1074
Sau3AI GATC 6 cut(s) 153, 344, 451, 550, 583, 922
Sau96I GGNCC 3 cut(s) 262, 686, 769
SchI GAGTC 4 cut(s) 125, 218, 825, 882
ScrFI CCNGG 1 cut(s) 738
SduI GDGCHC 2 cut(s) 598, 840
SfaNI GCATC 1 cut(s) 856
SfcI CTRYAG 1 cut(s) 428
SfoI GGCGCC 1 cut(s) 1111
SfuI TTCGAA 1 cut(s) 568
SgrAI CRCCGGYG 1 cut(s) 146
SinI GGWCC 1 cut(s) 686
SmiMI CAYNNNNRTG 1 cut(s) 759
SmlI CTYRAG 3 cut(s) 280, 504, 806
SmoI CTYRAG 3 cut(s) 280, 504, 806
Sse9I AATT 6 cut(s) 310, 328, 351, 791, 852, 987
SsiI CCGC 2 cut(s) 81, 150
SspDI GGCGCC 1 cut(s) 1109
SspMI CTAG 2 cut(s) 29, 441
SstI GAGCTC 1 cut(s) 840
StyD4I CCNGG 1 cut(s) 736
TaaI ACNGT 2 cut(s) 191, 525
TaiI ACGT 3 cut(s) 92, 533, 643
TaqI TCGA 5 cut(s) 134, 378, 460, 568, 747
TasI AATT 6 cut(s) 310, 328, 351, 791, 852, 987
TfiI GAWTC 4 cut(s) 175, 472, 601, 1129
Tru1I TTAA 1 cut(s) 726
Tru9I TTAA 1 cut(s) 726
TscAI CASTG 3 cut(s) 528, 688, 1088
TseI GCWGC 6 cut(s) 25, 161, 802, 848, 875, 1073
TspDTI ATGAA 5 cut(s) 241, 765, 924, 944, 1023
TspRI CASTG 3 cut(s) 528, 688, 1088
Tth111I GACNNNGTC 1 cut(s) 815
Van91I CCANNNNNTGG 1 cut(s) 1083
VneI GTGCAC 1 cut(s) 594
VpaK11BI GGWCC 1 cut(s) 686
XapI RAATTY 2 cut(s) 328, 791
XbaI TCTAGA 1 cut(s) 440
XcmI CCANNNNNNNNNTGG 2 cut(s) 971, 1084
XmiI GTMKAC 1 cut(s) 527
XmnI GAANNNNTTC 1 cut(s) 1030
XspI CTAG 2 cut(s) 29, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.