Rh2BG659000

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002827
Physical Location & Seq
Reverse (-)
87508261 .. 87509780
1520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG659000.1

Sequence Viewer

Length: 1173 bp
ATGGAGATGGAAAGATTTTTGGTTGTGAGCATTTTGGTAACATGTGTGCTAGTAATCAGTGTGGGAGGTGAAGAAGATGCATCGTCACCTTCGGCTTCAGTTTCGGCTTGTGAGTTTCCAGCAATCTATAACTTTGGAGACTCGAATTCAGATACCGGTGGTATATCCGCTGCATTCTACGCAATGGCATCACCCTACGGTGAAACTTTCTTCCACAGACCAGCTGGAAGGGCCAGTGATGGTCGTCTAATAATAGATTTTATAGCCGAGAAGCTGGGGTTGCCATACCTGAGTTCATATTTGGACTCAATTCGGAGCAATTTCAGACACGGTGCCAATTTTGCTTCAGGAGGAGCAACCATCAGGCGGCAGAATGAGTCATGGTTTGAGAATGGTGTGAGTCCTTTTCCCCTTGACATCCAGTTTGTGCAGTATGATCAGTTCAAGGCGCGTACCACCACTCTTTACAACCAAGCTCAGGATCTTTCTGACATACAAGATCTACCCAGAGCAGAAGACTTCTCAAAGGCTCTTTACACATTCGATATAGGCCAAAATGATCTAGCTGCTGGTTTTCGGAAAATGAGTACCGAGCAATTTCTAGCAGAAATTCCAGACATTGTTAACCAGTTGGCCACAGCCATCCGAAGTCTGTATGACCGAGGGGCAAGGGCATTCTGGATACACAACACTGGTCCAATAGGTTGCTTGGGAGTGACATTACACTACATCCATGACCCGAGCCCTCCCGGATACCTTGATCGCCACGGTTGCGTGAAGTTTCAAAACCACATGGCGAGACAGTTCAATAGGCTGCTCAAACAGAAAGTGATCCAACTAAGGAAGGAACTCCCTCTTTCTGCAATAACTTATGTCAATGTCTTTGCTGCTAAGTATAAGCTCATCAGCAATGCAAAGAAGCTTGGTTTTTTCGATAAAACGAAGATCTGCTGTGGATATCATGAAGATTATGACCATGTGTGGTGTGGTAATAAGGGAAAGATAAATGGGAGTGAAATATATGCAGGTTCATGTGAAGATCCTTCCCTGTATATAAGCTGGGATGGCGTGCACTATAGTGAGGCTGCAAATCATTGGATTGCTAATCAGATTGCCAGTGGTTCATTCTCAGATCCACCAGTTTCAATCATAAATGCATGTCATAGGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

390

Amino Acids

43.37

Weight (kDa)

6.06

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 42 - 371 2.9e-45 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1016
AccB1I GGYRCC 1 cut(s) 332
AccII CGCG 1 cut(s) 451
AciI CCGC 2 cut(s) 168, 367
AclWI GGATC 4 cut(s) 489, 826, 1034, 1127
AcoI YGGCCR 1 cut(s) 633
AcsI RAATTY 2 cut(s) 145, 609
AcuI CTGAAG 2 cut(s) 81, 330
AfaI GTAC 2 cut(s) 454, 589
AfiI CCNNNNNNNGG 2 cut(s) 366, 478
AflIII ACRYGT 1 cut(s) 41
AgeI ACCGGT 1 cut(s) 155
AgsI TTSAA 4 cut(s) 445, 785, 808, 1146
AleI CACNNNNGTG 1 cut(s) 1077
AluBI AGCT 7 cut(s) 224, 274, 476, 566, 901, 922, 1059
AluI AGCT 7 cut(s) 224, 274, 476, 566, 901, 922, 1059
Alw21I GWGCWC 1 cut(s) 1074
Alw26I GTCTC 2 cut(s) 132, 793
Alw44I GTGCAC 1 cut(s) 1070
AlwI GGATC 4 cut(s) 489, 826, 1034, 1127
Ama87I CYCGRG 1 cut(s) 739
AoxI GGCC 4 cut(s) 231, 550, 633, 1166
ApaLI GTGCAC 1 cut(s) 1070
ApeKI GCWGC 5 cut(s) 170, 566, 814, 887, 1085
ApoI RAATTY 2 cut(s) 145, 609
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
AsiGI ACCGGT 1 cut(s) 155
AspLEI GCGC 1 cut(s) 451
AspS9I GGNCC 2 cut(s) 231, 695
AsuC2I CCSGG 1 cut(s) 750
AsuHPI GGTGA 4 cut(s) 78, 80, 183, 212
AvaI CYCGRG 1 cut(s) 739
AvaII GGWCC 1 cut(s) 695
BaeGI GKGCMC 1 cut(s) 1074
BalI TGGCCA 1 cut(s) 635
BanI GGYRCC 1 cut(s) 332
BanII GRGCYC 1 cut(s) 746
BbsI GAAGAC 1 cut(s) 522
Bbv12I GWGCWC 1 cut(s) 1074
BbvI GCAGC 5 cut(s) 157, 553, 801, 874, 1072
BccI CCATC 4 cut(s) 233, 368, 650, 1058
BciVI GTATCC 2 cut(s) 675, 746
BclI TGATCA 1 cut(s) 436
BcnI CCSGG 1 cut(s) 750
BcoDI GTCTC 2 cut(s) 132, 793
BfaI CTAG 3 cut(s) 50, 563, 602
BfmI CTRYAG 1 cut(s) 1075
BfuAI ACCTGC 1 cut(s) 1016
BfuI GTATCC 2 cut(s) 675, 746
BglII AGATCT 2 cut(s) 499, 945
BisI GCNGC 6 cut(s) 171, 368, 567, 815, 888, 1086
BlsI GCNGC 6 cut(s) 172, 369, 568, 816, 889, 1087
Bme1390I CCNGG 1 cut(s) 750
Bme18I GGWCC 1 cut(s) 695
BmeT110I CYCGRG 1 cut(s) 739
BmgT120I GGNCC 2 cut(s) 231, 695
BmiI GGNNCC 1 cut(s) 334
BmrFI CCNGG 1 cut(s) 750
BmsI GCATC 3 cut(s) 67, 89, 197
BpiI GAAGAC 1 cut(s) 522
Bpu10I CCTNAGC 1 cut(s) 477
BpuMI CCSGG 1 cut(s) 750
BsaJI CCNNGG 2 cut(s) 661, 766
BsaWI WCCGGW 1 cut(s) 155
Bsc4I CCNNNNNNNGG 2 cut(s) 366, 478
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 6 cut(s) 234, 421, 628, 697, 1116, 1139
Bse3DI GCAATG 2 cut(s) 189, 916
BseDI CCNNGG 2 cut(s) 661, 766
BseGI GGATG 4 cut(s) 417, 642, 729, 1069
BseLI CCNNNNNNNGG 2 cut(s) 366, 478
BseMI GCAATG 2 cut(s) 189, 916
BseMII CTCAG 3 cut(s) 281, 491, 1143
BseNI ACTGG 6 cut(s) 234, 421, 628, 697, 1116, 1139
BseRI GAGGAG 1 cut(s) 366
BseSI GKGCMC 1 cut(s) 1074
BseXI GCAGC 5 cut(s) 157, 553, 801, 874, 1072
BseYI CCCAGC 2 cut(s) 274, 1059
BsgI GTGCAG 1 cut(s) 449
Bsh1236I CGCG 1 cut(s) 451
BshFI GGCC 4 cut(s) 233, 552, 635, 1168
BshNI GGYRCC 1 cut(s) 332
BshTI ACCGGT 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 1074
BsiHKCI CYCGRG 1 cut(s) 739
BsiSI CCGG 2 cut(s) 156, 750
BslI CCNNNNNNNGG 2 cut(s) 366, 478
BsmAI GTCTC 2 cut(s) 132, 793
BsmI GAATGC 2 cut(s) 173, 674
BsnI GGCC 4 cut(s) 233, 552, 635, 1168
BsoBI CYCGRG 1 cut(s) 739
Bsp1286I GDGCHC 2 cut(s) 746, 1074
Bsp143I GATC 9 cut(s) 436, 481, 499, 559, 760, 831, 945, 1039, 1132
BspACI CCGC 2 cut(s) 168, 367
BspANI GGCC 4 cut(s) 233, 552, 635, 1168
BspCNI CTCAG 3 cut(s) 282, 490, 1142
BspFNI CGCG 1 cut(s) 451
BspHI TCATGA 1 cut(s) 961
BspLI GGNNCC 1 cut(s) 334
BspMI ACCTGC 1 cut(s) 1016
BspPI GGATC 4 cut(s) 489, 826, 1034, 1127
BspT107I GGYRCC 1 cut(s) 332
BsrDI GCAATG 2 cut(s) 189, 916
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 6 cut(s) 234, 421, 628, 697, 1116, 1139
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 2 cut(s) 661, 766
BssMI GATC 9 cut(s) 436, 481, 499, 559, 760, 831, 945, 1039, 1132
Bst4CI ACNGT 4 cut(s) 200, 332, 770, 804
BstC8I GCNNGC 1 cut(s) 1070
BstDEI CTNAG 5 cut(s) 290, 477, 839, 891, 1129
BstDSI CCRYGG 1 cut(s) 766
BstF5I GGATG 4 cut(s) 417, 642, 729, 1069
BstFNI CGCG 1 cut(s) 451
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 9 cut(s) 439, 484, 502, 562, 763, 834, 948, 1042, 1135
BstMAI GTCTC 2 cut(s) 132, 793
BstMBI GATC 9 cut(s) 436, 481, 499, 559, 760, 831, 945, 1039, 1132
BstMWI GCNNNNNNNGC 6 cut(s) 179, 230, 280, 341, 771, 1065
BstNSI RCATGY 2 cut(s) 45, 1161
BstSCI CCNGG 1 cut(s) 748
BstSFI CTRYAG 1 cut(s) 1075
BstSLI GKGCMC 1 cut(s) 1074
BstUI CGCG 1 cut(s) 451
BstV1I GCAGC 5 cut(s) 157, 553, 801, 874, 1072
BstV2I GAAGAC 1 cut(s) 522
BstX2I RGATCY 5 cut(s) 481, 499, 945, 1039, 1132
BstYI RGATCY 5 cut(s) 481, 499, 945, 1039, 1132
BsuI GTATCC 2 cut(s) 675, 746
BsuRI GGCC 4 cut(s) 233, 552, 635, 1168
BtgI CCRYGG 1 cut(s) 766
BtsCI GGATG 4 cut(s) 417, 642, 729, 1069
BtsIMutI CAGTG 4 cut(s) 64, 241, 690, 1123
BveI ACCTGC 1 cut(s) 1016
Cac8I GCNNGC 1 cut(s) 1070
CciI TCATGA 1 cut(s) 961
CfoI GCGC 1 cut(s) 451
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 2 cut(s) 231, 695
Csp6I GTAC 2 cut(s) 453, 588
CspAI ACCGGT 1 cut(s) 155
CviAII CATG 8 cut(s) 42, 381, 734, 793, 962, 977, 1032, 1158
CviQI GTAC 2 cut(s) 453, 588
DdeI CTNAG 5 cut(s) 290, 477, 839, 891, 1129
DpnI GATC 9 cut(s) 438, 483, 501, 561, 762, 833, 947, 1041, 1134
DpnII GATC 9 cut(s) 436, 481, 499, 559, 760, 831, 945, 1039, 1132
EaeI YGGCCR 1 cut(s) 633
Eco147I AGGCCT 1 cut(s) 1168
Eco24I GRGCYC 1 cut(s) 746
Eco32I GATATC 1 cut(s) 959
Eco47I GGWCC 1 cut(s) 695
Eco57I CTGAAG 2 cut(s) 81, 330
Eco88I CYCGRG 1 cut(s) 739
EcoRI GAATTC 1 cut(s) 145
EcoRV GATATC 1 cut(s) 959
EcoT22I ATGCAT 2 cut(s) 82, 1159
EcoT38I GRGCYC 1 cut(s) 746
FaeI CATG 8 cut(s) 45, 384, 737, 796, 965, 980, 1035, 1161
FatI CATG 8 cut(s) 41, 380, 733, 792, 961, 976, 1031, 1157
FbaI TGATCA 1 cut(s) 436
Fnu4HI GCNGC 6 cut(s) 171, 368, 567, 815, 888, 1086
FokI GGATG 4 cut(s) 404, 629, 716, 1076
FriOI GRGCYC 1 cut(s) 746
Fsp4HI GCNGC 6 cut(s) 171, 368, 567, 815, 888, 1086
FspBI CTAG 3 cut(s) 50, 563, 602
GlaI GCGC 1 cut(s) 450
GluI GCNGC 6 cut(s) 171, 368, 567, 815, 888, 1086
GsaI CCCAGC 2 cut(s) 278, 1063
HaeIII GGCC 4 cut(s) 233, 552, 635, 1168
HapII CCGG 2 cut(s) 156, 750
HhaI GCGC 1 cut(s) 451
Hin1II CATG 8 cut(s) 45, 384, 737, 796, 965, 980, 1035, 1161
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HindIII AAGCTT 1 cut(s) 920
HinfI GANTC 4 cut(s) 140, 305, 377, 400
HpaI GTTAAC 1 cut(s) 625
HpaII CCGG 2 cut(s) 156, 750
HphI GGTGA 4 cut(s) 78, 80, 183, 212
Hpy166II GTNNAC 2 cut(s) 625, 1072
Hpy188I TCNGA 8 cut(s) 151, 315, 326, 490, 579, 647, 1110, 1132
Hpy188III TCNNGA 5 cut(s) 348, 479, 614, 679, 962
Hpy8I GTNNAC 2 cut(s) 625, 1072
HpyAV CCTTC 4 cut(s) 99, 222, 838, 1053
HpyCH4III ACNGT 4 cut(s) 200, 332, 770, 804
HpyCH4V TGCA 9 cut(s) 80, 173, 430, 863, 914, 1025, 1072, 1088, 1157
HpyF10VI GCNNNNNNNGC 6 cut(s) 179, 230, 280, 341, 771, 1065
HpyF3I CTNAG 5 cut(s) 290, 477, 839, 891, 1129
Hsp92II CATG 8 cut(s) 45, 384, 737, 796, 965, 980, 1035, 1161
HspAI GCGC 1 cut(s) 449
Ksp22I TGATCA 1 cut(s) 436
KspAI GTTAAC 1 cut(s) 625
Kzo9I GATC 9 cut(s) 436, 481, 499, 559, 760, 831, 945, 1039, 1132
LmnI GCTCC 2 cut(s) 315, 353
Lsp1109I GCAGC 5 cut(s) 157, 553, 801, 874, 1072
LweI GCATC 3 cut(s) 67, 89, 197
MaeI CTAG 3 cut(s) 50, 563, 602
MaeIII GTNAC 3 cut(s) 37, 84, 715
MalI GATC 9 cut(s) 438, 483, 501, 561, 762, 833, 947, 1041, 1134
MboI GATC 9 cut(s) 436, 481, 499, 559, 760, 831, 945, 1039, 1132
MboII GAAGA 7 cut(s) 83, 86, 202, 527, 955, 977, 1049
MflI RGATCY 5 cut(s) 481, 499, 945, 1039, 1132
MhlI GDGCHC 2 cut(s) 746, 1074
MlsI TGGCCA 1 cut(s) 635
MluCI AATT 6 cut(s) 145, 309, 319, 337, 596, 609
MluNI TGGCCA 1 cut(s) 635
MlyI GAGTC 4 cut(s) 134, 299, 386, 409
MmeI TCCRAC 1 cut(s) 859
MnlI CCTC 6 cut(s) 59, 344, 656, 756, 864, 1075
Mox20I TGGCCA 1 cut(s) 635
Mph1103I ATGCAT 2 cut(s) 82, 1159
MscI TGGCCA 1 cut(s) 635
MseI TTAA 2 cut(s) 624, 1171
MslI CAYNNNNRTG 1 cut(s) 1077
Msp20I TGGCCA 1 cut(s) 635
MspA1I CMGCKG 2 cut(s) 170, 224
MspI CCGG 2 cut(s) 156, 750
MspR9I CCNGG 1 cut(s) 750
Mva1269I GAATGC 2 cut(s) 173, 674
MvnI CGCG 1 cut(s) 451
MwoI GCNNNNNNNGC 6 cut(s) 179, 230, 280, 341, 771, 1065
NciI CCSGG 1 cut(s) 750
NdeII GATC 9 cut(s) 436, 481, 499, 559, 760, 831, 945, 1039, 1132
NlaIII CATG 8 cut(s) 45, 384, 737, 796, 965, 980, 1035, 1161
NlaIV GGNNCC 1 cut(s) 334
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 2 cut(s) 84, 715
NsiI ATGCAT 2 cut(s) 82, 1159
NspI RCATGY 2 cut(s) 45, 1161
OliI CACNNNNGTG 1 cut(s) 1077
PagI TCATGA 1 cut(s) 961
PceI AGGCCT 1 cut(s) 1168
PciI ACATGT 1 cut(s) 41
PctI GAATGC 2 cut(s) 173, 674
PfoI TCCNGGA 1 cut(s) 748
PinAI ACCGGT 1 cut(s) 155
PkrI GCNGC 6 cut(s) 172, 369, 568, 816, 889, 1087
PleI GAGTC 4 cut(s) 134, 299, 385, 408
PpsI GAGTC 4 cut(s) 134, 299, 385, 408
PscI ACATGT 1 cut(s) 41
PspFI CCCAGC 2 cut(s) 274, 1059
PspN4I GGNNCC 1 cut(s) 334
PspPI GGNCC 2 cut(s) 231, 695
PsrI GAACNNNNNNTAC 2 cut(s) 425, 457
PsuI RGATCY 5 cut(s) 481, 499, 945, 1039, 1132
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 2 cut(s) 454, 589
RsaNI GTAC 2 cut(s) 453, 588
RseI CAYNNNNRTG 1 cut(s) 1077
SaqAI TTAA 2 cut(s) 624, 1171
SatI GCNGC 6 cut(s) 171, 368, 567, 815, 888, 1086
Sau3AI GATC 9 cut(s) 436, 481, 499, 559, 760, 831, 945, 1039, 1132
Sau96I GGNCC 2 cut(s) 231, 695
SchI GAGTC 4 cut(s) 134, 299, 386, 409
ScrFI CCNGG 1 cut(s) 750
SduI GDGCHC 2 cut(s) 746, 1074
SfaNI GCATC 3 cut(s) 67, 89, 197
SfcI CTRYAG 1 cut(s) 1075
SinI GGWCC 1 cut(s) 695
SmiMI CAYNNNNRTG 1 cut(s) 1077
Sse9I AATT 6 cut(s) 145, 309, 319, 337, 596, 609
SseBI AGGCCT 1 cut(s) 1168
SsiI CCGC 2 cut(s) 168, 367
SspMI CTAG 3 cut(s) 50, 563, 602
StuI AGGCCT 1 cut(s) 1168
StyD4I CCNGG 1 cut(s) 748
TaaI ACNGT 4 cut(s) 200, 332, 770, 804
TaqI TCGA 3 cut(s) 143, 543, 933
TaqII GACCGA 1 cut(s) 675
TasI AATT 6 cut(s) 145, 309, 319, 337, 596, 609
TauI GCSGC 1 cut(s) 370
Tru1I TTAA 2 cut(s) 624, 1171
Tru9I TTAA 2 cut(s) 624, 1171
TscAI CASTG 4 cut(s) 64, 241, 697, 1123
TseFI GTSAC 2 cut(s) 84, 715
TseI GCWGC 5 cut(s) 170, 566, 814, 887, 1085
Tsp45I GTSAC 2 cut(s) 84, 715
TspDTI ATGAA 4 cut(s) 285, 978, 1020, 1113
TspRI CASTG 4 cut(s) 64, 241, 697, 1123
VneI GTGCAC 1 cut(s) 1070
VpaK11BI GGWCC 1 cut(s) 695
XapI RAATTY 2 cut(s) 145, 609
XceI RCATGY 2 cut(s) 45, 1161
XcmI CCANNNNNNNNNTGG 2 cut(s) 221, 983
XspI CTAG 3 cut(s) 50, 563, 602
Zsp2I ATGCAT 2 cut(s) 82, 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.