RchiOBHm_Chr2g0173391

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002827
Physical Location & Seq
Reverse (-)
86424582 .. 86426134
1553 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54061

Sequence Viewer

Length: 1206 bp
ATGAGTACATACATGTTTAGTGCAGTAGAGAAAATGGAGATGGAAAGATTTTTGGTTGTGAGCATTTTGGTAACATGTGTGCTAGTAATCAGTGTGGGAGGTGAAGAAGATGCATCGTCACCTTCGGCTTCAGTTTCGGCTTGTGAGTTTCCAGCAATCTATAACTTTGGAGACTCGAATTCAGATACCGGTGGTATATCCGCTGCATTCTACGCAATGGCATCACCCTACGGTGAAACTTTCTTCCACAGACCAGCTGGAAGGGCCAGTGATGGTCGTCTAATAATAGATTTTATAGCCGAGAAGCTGGGGGTGCCATACCTGAGTTCATATTTGGACTCAATTCGGAGCAATTTCAGACACGGTGCCAATTTTGCTTCAGGAGGAGCAACCATCAGGCGGCAGAATGAGTCATGGTTTGAGAATGGTGTGAGTCCTTTTCCCCTTGACATCCAGTTTGTGCAGTATGATCAGTTCAAGGCGCGTACCACCACTCTTTACAACCAAGCTAAGGATCTTTCTGACATACAAGATCTACCCAGAGCAGAAGACTTCTCAAAGGCTCTTTACACATTCGATATAGGCCAAAATGATCTAGCTGCTGGTTTTCGGAAAATGAGTACCGAGCAATTTCTAGCAGAAATTCCAGACATTGTTAACCAGTTGGCCACAGCCATCCGAAGTTTGTATGACCGAGGGGCAAGGGCATTCTGGATACACAACACTGGTCCAATAGGTTGCTTGGGAGTGACATTACACTACATCCATGACCCGAGCCCTCCCGGATACCTTGATCGCCACGGTTGCGTGAAGTTACAAAACCACATGGCGAGACAGTTCAATAGGCTGCTCAAACAGAAAGTGATCCAACTAAGGAAGGAACTCCCTCTTTCTGCAATAACTTATGTCAATGTCTTTGCTGCTAAGTATAAGCTCATCAGCAATGCAAAGAAGCTTGGTTTTTTCGATAAAACGAAGATCTGCTGTGGATATCATGAAGATTATGACCATGTGTGGTGTGGTAATAAGGGAAAGATAAATGGGAGTGAAATATATGCAGGTTCATGTGAAGATCCTTCCCTGTATATAAGCTGGGATGGCGTGCACTATAGTGAGGCTGCAAATCATTGGATTGCTAATCAGATTGCCAGTGGTTCATTCTCAGATCCACCAGTTTCAATCATAAATGCATGTCATAGGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

401

Amino Acids

44.6

Weight (kDa)

6.18

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 53 - 382 1.6e-45 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1049
AccB1I GGYRCC 2 cut(s) 313, 365
AccII CGCG 1 cut(s) 484
AciI CCGC 2 cut(s) 201, 400
AclWI GGATC 4 cut(s) 522, 859, 1067, 1160
AcoI YGGCCR 1 cut(s) 666
AcsI RAATTY 2 cut(s) 178, 642
AcuI CTGAAG 2 cut(s) 114, 363
AfaI GTAC 3 cut(s) 7, 487, 622
AfiI CCNNNNNNNGG 2 cut(s) 399, 511
AflIII ACRYGT 2 cut(s) 12, 74
AgeI ACCGGT 1 cut(s) 188
AgsI TTSAA 3 cut(s) 478, 841, 1179
AleI CACNNNNGTG 1 cut(s) 1110
AluBI AGCT 7 cut(s) 257, 307, 509, 599, 934, 955, 1092
AluI AGCT 7 cut(s) 257, 307, 509, 599, 934, 955, 1092
Alw21I GWGCWC 1 cut(s) 1107
Alw26I GTCTC 2 cut(s) 165, 826
Alw44I GTGCAC 1 cut(s) 1103
AlwI GGATC 4 cut(s) 522, 859, 1067, 1160
Ama87I CYCGRG 1 cut(s) 772
AoxI GGCC 4 cut(s) 264, 583, 666, 1199
ApaLI GTGCAC 1 cut(s) 1103
ApeKI GCWGC 5 cut(s) 203, 599, 847, 920, 1118
ApoI RAATTY 2 cut(s) 178, 642
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
AsiGI ACCGGT 1 cut(s) 188
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 2 cut(s) 264, 728
AsuC2I CCSGG 1 cut(s) 783
AsuHPI GGTGA 4 cut(s) 111, 113, 216, 245
AvaI CYCGRG 1 cut(s) 772
AvaII GGWCC 1 cut(s) 728
BaeGI GKGCMC 1 cut(s) 1107
BalI TGGCCA 1 cut(s) 668
BanI GGYRCC 2 cut(s) 313, 365
BanII GRGCYC 1 cut(s) 779
BbsI GAAGAC 1 cut(s) 555
Bbv12I GWGCWC 1 cut(s) 1107
BbvI GCAGC 5 cut(s) 190, 586, 834, 907, 1105
BccI CCATC 5 cut(s) 34, 266, 401, 683, 1091
BciVI GTATCC 2 cut(s) 708, 779
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 1 cut(s) 783
BcoDI GTCTC 2 cut(s) 165, 826
BfaI CTAG 3 cut(s) 83, 596, 635
BfmI CTRYAG 1 cut(s) 1108
BfuAI ACCTGC 1 cut(s) 1049
BfuI GTATCC 2 cut(s) 708, 779
BglII AGATCT 2 cut(s) 532, 978
BisI GCNGC 6 cut(s) 204, 401, 600, 848, 921, 1119
BlsI GCNGC 6 cut(s) 205, 402, 601, 849, 922, 1120
Bme1390I CCNGG 1 cut(s) 783
Bme18I GGWCC 1 cut(s) 728
BmeT110I CYCGRG 1 cut(s) 772
BmgT120I GGNCC 2 cut(s) 264, 728
BmiI GGNNCC 2 cut(s) 315, 367
BmrFI CCNGG 1 cut(s) 783
BmsI GCATC 3 cut(s) 100, 122, 230
BpiI GAAGAC 1 cut(s) 555
Bpu10I CCTNAGC 1 cut(s) 510
BpuMI CCSGG 1 cut(s) 783
BsaJI CCNNGG 2 cut(s) 694, 799
BsaWI WCCGGW 1 cut(s) 188
Bsc4I CCNNNNNNNGG 2 cut(s) 399, 511
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 6 cut(s) 267, 454, 661, 730, 1149, 1172
Bse3DI GCAATG 2 cut(s) 222, 949
BseDI CCNNGG 2 cut(s) 694, 799
BseGI GGATG 4 cut(s) 450, 675, 762, 1102
BseLI CCNNNNNNNGG 2 cut(s) 399, 511
BseMI GCAATG 2 cut(s) 222, 949
BseMII CTCAG 2 cut(s) 314, 1176
BseNI ACTGG 6 cut(s) 267, 454, 661, 730, 1149, 1172
BseRI GAGGAG 1 cut(s) 399
BseSI GKGCMC 1 cut(s) 1107
BseXI GCAGC 5 cut(s) 190, 586, 834, 907, 1105
BseYI CCCAGC 2 cut(s) 307, 1092
BsgI GTGCAG 2 cut(s) 42, 482
Bsh1236I CGCG 1 cut(s) 484
BshFI GGCC 4 cut(s) 266, 585, 668, 1201
BshNI GGYRCC 2 cut(s) 313, 365
BshTI ACCGGT 1 cut(s) 188
BsiHKAI GWGCWC 1 cut(s) 1107
BsiHKCI CYCGRG 1 cut(s) 772
BsiSI CCGG 2 cut(s) 189, 783
BslI CCNNNNNNNGG 2 cut(s) 399, 511
BsmAI GTCTC 2 cut(s) 165, 826
BsmI GAATGC 2 cut(s) 206, 707
BsnI GGCC 4 cut(s) 266, 585, 668, 1201
BsoBI CYCGRG 1 cut(s) 772
Bsp1286I GDGCHC 2 cut(s) 779, 1107
Bsp143I GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
BspACI CCGC 2 cut(s) 201, 400
BspANI GGCC 4 cut(s) 266, 585, 668, 1201
BspCNI CTCAG 2 cut(s) 315, 1175
BspFNI CGCG 1 cut(s) 484
BspHI TCATGA 1 cut(s) 994
BspLI GGNNCC 2 cut(s) 315, 367
BspMI ACCTGC 1 cut(s) 1049
BspPI GGATC 4 cut(s) 522, 859, 1067, 1160
BspT107I GGYRCC 2 cut(s) 313, 365
BsrDI GCAATG 2 cut(s) 222, 949
BsrFI RCCGGY 1 cut(s) 188
BsrI ACTGG 6 cut(s) 267, 454, 661, 730, 1149, 1172
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 2 cut(s) 694, 799
BssMI GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
Bst4CI ACNGT 4 cut(s) 233, 365, 803, 837
BstC8I GCNNGC 1 cut(s) 1103
BstDEI CTNAG 5 cut(s) 323, 510, 872, 924, 1162
BstDSI CCRYGG 1 cut(s) 799
BstF5I GGATG 4 cut(s) 450, 675, 762, 1102
BstFNI CGCG 1 cut(s) 484
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 9 cut(s) 472, 517, 535, 595, 796, 867, 981, 1075, 1168
BstMAI GTCTC 2 cut(s) 165, 826
BstMBI GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
BstMWI GCNNNNNNNGC 6 cut(s) 212, 263, 313, 374, 804, 1098
BstNSI RCATGY 3 cut(s) 16, 78, 1194
BstSCI CCNGG 1 cut(s) 781
BstSFI CTRYAG 1 cut(s) 1108
BstSLI GKGCMC 1 cut(s) 1107
BstUI CGCG 1 cut(s) 484
BstV1I GCAGC 5 cut(s) 190, 586, 834, 907, 1105
BstV2I GAAGAC 1 cut(s) 555
BstX2I RGATCY 5 cut(s) 514, 532, 978, 1072, 1165
BstYI RGATCY 5 cut(s) 514, 532, 978, 1072, 1165
BsuI GTATCC 2 cut(s) 708, 779
BsuRI GGCC 4 cut(s) 266, 585, 668, 1201
BtgI CCRYGG 1 cut(s) 799
BtsCI GGATG 4 cut(s) 450, 675, 762, 1102
BtsIMutI CAGTG 4 cut(s) 97, 274, 723, 1156
BveI ACCTGC 1 cut(s) 1049
Cac8I GCNNGC 1 cut(s) 1103
CciI TCATGA 1 cut(s) 994
CfoI GCGC 1 cut(s) 484
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 2 cut(s) 264, 728
Csp6I GTAC 3 cut(s) 6, 486, 621
CspAI ACCGGT 1 cut(s) 188
CviAII CATG 9 cut(s) 13, 75, 414, 767, 826, 995, 1010, 1065, 1191
CviQI GTAC 3 cut(s) 6, 486, 621
DdeI CTNAG 5 cut(s) 323, 510, 872, 924, 1162
DpnI GATC 9 cut(s) 471, 516, 534, 594, 795, 866, 980, 1074, 1167
DpnII GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
EaeI YGGCCR 1 cut(s) 666
Eco147I AGGCCT 1 cut(s) 1201
Eco24I GRGCYC 1 cut(s) 779
Eco32I GATATC 1 cut(s) 992
Eco47I GGWCC 1 cut(s) 728
Eco57I CTGAAG 2 cut(s) 114, 363
Eco88I CYCGRG 1 cut(s) 772
EcoRI GAATTC 1 cut(s) 178
EcoRV GATATC 1 cut(s) 992
EcoT22I ATGCAT 2 cut(s) 115, 1192
EcoT38I GRGCYC 1 cut(s) 779
FaeI CATG 9 cut(s) 16, 78, 417, 770, 829, 998, 1013, 1068, 1194
FatI CATG 9 cut(s) 12, 74, 413, 766, 825, 994, 1009, 1064, 1190
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 6 cut(s) 204, 401, 600, 848, 921, 1119
FokI GGATG 4 cut(s) 437, 662, 749, 1109
FriOI GRGCYC 1 cut(s) 779
Fsp4HI GCNGC 6 cut(s) 204, 401, 600, 848, 921, 1119
FspBI CTAG 3 cut(s) 83, 596, 635
GlaI GCGC 1 cut(s) 483
GluI GCNGC 6 cut(s) 204, 401, 600, 848, 921, 1119
GsaI CCCAGC 2 cut(s) 311, 1096
HaeIII GGCC 4 cut(s) 266, 585, 668, 1201
HapII CCGG 2 cut(s) 189, 783
HhaI GCGC 1 cut(s) 484
Hin1II CATG 9 cut(s) 16, 78, 417, 770, 829, 998, 1013, 1068, 1194
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HindIII AAGCTT 1 cut(s) 953
HinfI GANTC 4 cut(s) 173, 338, 410, 433
HpaI GTTAAC 1 cut(s) 658
HpaII CCGG 2 cut(s) 189, 783
HphI GGTGA 4 cut(s) 111, 113, 216, 245
Hpy166II GTNNAC 2 cut(s) 658, 1105
Hpy188I TCNGA 8 cut(s) 184, 348, 359, 523, 612, 680, 1143, 1165
Hpy188III TCNNGA 4 cut(s) 381, 647, 712, 995
Hpy8I GTNNAC 2 cut(s) 658, 1105
HpyAV CCTTC 4 cut(s) 132, 255, 871, 1086
HpyCH4III ACNGT 4 cut(s) 233, 365, 803, 837
HpyF10VI GCNNNNNNNGC 6 cut(s) 212, 263, 313, 374, 804, 1098
HpyF3I CTNAG 5 cut(s) 323, 510, 872, 924, 1162
Hsp92II CATG 9 cut(s) 16, 78, 417, 770, 829, 998, 1013, 1068, 1194
HspAI GCGC 1 cut(s) 482
Ksp22I TGATCA 1 cut(s) 469
KspAI GTTAAC 1 cut(s) 658
Kzo9I GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
LmnI GCTCC 2 cut(s) 348, 386
Lsp1109I GCAGC 5 cut(s) 190, 586, 834, 907, 1105
LweI GCATC 3 cut(s) 100, 122, 230
MaeI CTAG 3 cut(s) 83, 596, 635
MaeIII GTNAC 4 cut(s) 70, 117, 748, 813
MalI GATC 9 cut(s) 471, 516, 534, 594, 795, 866, 980, 1074, 1167
MboI GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
MboII GAAGA 7 cut(s) 116, 119, 235, 560, 988, 1010, 1082
MflI RGATCY 5 cut(s) 514, 532, 978, 1072, 1165
MhlI GDGCHC 2 cut(s) 779, 1107
MlsI TGGCCA 1 cut(s) 668
MluCI AATT 6 cut(s) 178, 342, 352, 370, 629, 642
MluNI TGGCCA 1 cut(s) 668
MlyI GAGTC 4 cut(s) 167, 332, 419, 442
MmeI TCCRAC 1 cut(s) 892
MnlI CCTC 6 cut(s) 92, 377, 689, 789, 897, 1108
Mox20I TGGCCA 1 cut(s) 668
Mph1103I ATGCAT 2 cut(s) 115, 1192
MscI TGGCCA 1 cut(s) 668
MseI TTAA 2 cut(s) 657, 1204
MslI CAYNNNNRTG 1 cut(s) 1110
Msp20I TGGCCA 1 cut(s) 668
MspA1I CMGCKG 2 cut(s) 203, 257
MspI CCGG 2 cut(s) 189, 783
MspR9I CCNGG 1 cut(s) 783
Mva1269I GAATGC 2 cut(s) 206, 707
MvnI CGCG 1 cut(s) 484
MwoI GCNNNNNNNGC 6 cut(s) 212, 263, 313, 374, 804, 1098
NciI CCSGG 1 cut(s) 783
NdeII GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
NlaIII CATG 9 cut(s) 16, 78, 417, 770, 829, 998, 1013, 1068, 1194
NlaIV GGNNCC 2 cut(s) 315, 367
NmeAIII GCCGAG 1 cut(s) 325
NmuCI GTSAC 2 cut(s) 117, 748
NsiI ATGCAT 2 cut(s) 115, 1192
NspI RCATGY 3 cut(s) 16, 78, 1194
OliI CACNNNNGTG 1 cut(s) 1110
PagI TCATGA 1 cut(s) 994
PceI AGGCCT 1 cut(s) 1201
PciI ACATGT 2 cut(s) 12, 74
PctI GAATGC 2 cut(s) 206, 707
PfoI TCCNGGA 1 cut(s) 781
PinAI ACCGGT 1 cut(s) 188
PkrI GCNGC 6 cut(s) 205, 402, 601, 849, 922, 1120
PleI GAGTC 4 cut(s) 167, 332, 418, 441
PpsI GAGTC 4 cut(s) 167, 332, 418, 441
PscI ACATGT 2 cut(s) 12, 74
PspFI CCCAGC 2 cut(s) 307, 1092
PspN4I GGNNCC 2 cut(s) 315, 367
PspPI GGNCC 2 cut(s) 264, 728
PsrI GAACNNNNNNTAC 2 cut(s) 458, 490
PsuI RGATCY 5 cut(s) 514, 532, 978, 1072, 1165
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 3 cut(s) 7, 487, 622
RsaNI GTAC 3 cut(s) 6, 486, 621
RseI CAYNNNNRTG 1 cut(s) 1110
SaqAI TTAA 2 cut(s) 657, 1204
SatI GCNGC 6 cut(s) 204, 401, 600, 848, 921, 1119
Sau3AI GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
Sau96I GGNCC 2 cut(s) 264, 728
SchI GAGTC 4 cut(s) 167, 332, 419, 442
ScrFI CCNGG 1 cut(s) 783
SduI GDGCHC 2 cut(s) 779, 1107
SfaNI GCATC 3 cut(s) 100, 122, 230
SfcI CTRYAG 1 cut(s) 1108
SinI GGWCC 1 cut(s) 728
SmiMI CAYNNNNRTG 1 cut(s) 1110
Sse9I AATT 6 cut(s) 178, 342, 352, 370, 629, 642
SseBI AGGCCT 1 cut(s) 1201
SsiI CCGC 2 cut(s) 201, 400
SspMI CTAG 3 cut(s) 83, 596, 635
StuI AGGCCT 1 cut(s) 1201
StyD4I CCNGG 1 cut(s) 781
TaaI ACNGT 4 cut(s) 233, 365, 803, 837
TaqI TCGA 3 cut(s) 176, 576, 966
TaqII GACCGA 1 cut(s) 708
TasI AATT 6 cut(s) 178, 342, 352, 370, 629, 642
TatI WGTACW 1 cut(s) 5
TauI GCSGC 1 cut(s) 403
Tru1I TTAA 2 cut(s) 657, 1204
Tru9I TTAA 2 cut(s) 657, 1204
TscAI CASTG 4 cut(s) 97, 274, 730, 1156
TseFI GTSAC 2 cut(s) 117, 748
TseI GCWGC 5 cut(s) 203, 599, 847, 920, 1118
Tsp45I GTSAC 2 cut(s) 117, 748
TspDTI ATGAA 4 cut(s) 318, 1011, 1053, 1146
TspRI CASTG 4 cut(s) 97, 274, 730, 1156
VneI GTGCAC 1 cut(s) 1103
VpaK11BI GGWCC 1 cut(s) 728
XapI RAATTY 2 cut(s) 178, 642
XceI RCATGY 3 cut(s) 16, 78, 1194
XcmI CCANNNNNNNNNTGG 2 cut(s) 254, 1016
XspI CTAG 3 cut(s) 83, 596, 635
Zsp2I ATGCAT 2 cut(s) 115, 1192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.