Rw2G053220

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002827
Physical Location & Seq
Reverse (-)
81046518 .. 81048070
1553 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G053220.1

Sequence Viewer

Length: 1206 bp
ATGAGTACATACATGTTTAGTGCAGTAGAGAAAATGGAGATGGAAAGATTTTTGGTTGTGAGCATTTTGGTAACATGTGTGCTAGTAATCAGTGTGGGAGGTGAAGAAGATGCATCGTCACCTTCGGCTTCAGTTTCGGCTTGTGAGTTTCCAGCAATCTATAACTTTGGAGACTCGAATTCAGATACCGGTGGTATATCCGCTGCATTCTACGCAATGGCATCACCCTACGGTGAAACTTTCTTCCACAGACCAGCTGGAAGGGCCAGTGATGGTCGTCTAATAATAGATTTTATAGCCGAGAAGCTGGGGTTGCCATACCTGAGTTCATATTTGGACTCAATTCGGAGCAATTTCAGACACGGTGCCAATTTTGCTTCAGGAGGAGCAACCATCAGGCGGCAGAATGAGTCATGGTTTGAGAATGGTGTGAGTCCTTTTCCCCTTGACATCCAGTTTGTGCAGTATGATCAGTTCAAGGCGCGTACCACCACTCTTTACAACCAAGCTAAGGATCTTTCTGACATACAAGATCTACCCAGAGCAGAAGACTTCTCAAAGGCTCTTTACACATTCGATATAGGCCAAAATGATCTAGCTGCTGGTTTTCGGAAAATGAGTACCGAGCAATTTCTATCAGAAATTCCAGACATTGTTAACCAGTTGGCCACAGCCATCCGAAGTTTGTATGACCGAGGGGCAAGGGCATTCTGGATACACAACACTGGTCCAATAGGTTGCTTGGGAGTGACATTACACTACATCCATGACCCGAGCCCTCCCGGATACCTTGATCGCCACGGTTGCGTGAAGTTTCAAAACCACATGGCGAGACAGTTCAATAGGCTGCTCAAACAGAAAGTGATCCAACTAAGGAAGGAACTCCCTCTTTCTGCAATAACTTATGTCAATGTCTTTGCTGCTAAGTATAAGCTCATCAGCAATGCAAAGAAGCTTGGTTTTTTCGATAAAACGAAGATCTGCTGTGGATTTCATGAAGATTATGAACATGTGTGGTGTGGTAATAAGGGAAAGATAAATGGGAGTGAAATATATGCAGGTTCATGTGAAGATCCTTCCCTGTATATAAGCTGGGATGGCGTGCACTATAGTGAGGCTGCAAATCATTGGATTGCTAATCAGATTGCCAGTGGTTCATTCTCAGATCCACCAGTTTCAATCATAAATGCATGTCATAGGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

401

Amino Acids

44.66

Weight (kDa)

6.18

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 53 - 382 8e-45 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1049
AccB1I GGYRCC 1 cut(s) 365
AccII CGCG 1 cut(s) 484
AciI CCGC 2 cut(s) 201, 400
AclWI GGATC 4 cut(s) 522, 859, 1067, 1160
AcoI YGGCCR 1 cut(s) 666
AcsI RAATTY 2 cut(s) 178, 642
AcuI CTGAAG 2 cut(s) 114, 363
AfaI GTAC 3 cut(s) 7, 487, 622
AfiI CCNNNNNNNGG 2 cut(s) 399, 511
AflIII ACRYGT 3 cut(s) 12, 74, 1009
AgeI ACCGGT 1 cut(s) 188
AgsI TTSAA 4 cut(s) 478, 818, 841, 1179
AleI CACNNNNGTG 1 cut(s) 1110
AluBI AGCT 7 cut(s) 257, 307, 509, 599, 934, 955, 1092
AluI AGCT 7 cut(s) 257, 307, 509, 599, 934, 955, 1092
Alw21I GWGCWC 1 cut(s) 1107
Alw26I GTCTC 2 cut(s) 165, 826
Alw44I GTGCAC 1 cut(s) 1103
AlwI GGATC 4 cut(s) 522, 859, 1067, 1160
Ama87I CYCGRG 1 cut(s) 772
AoxI GGCC 4 cut(s) 264, 583, 666, 1199
ApaLI GTGCAC 1 cut(s) 1103
ApeKI GCWGC 5 cut(s) 203, 599, 847, 920, 1118
ApoI RAATTY 2 cut(s) 178, 642
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
AsiGI ACCGGT 1 cut(s) 188
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 2 cut(s) 264, 728
AsuC2I CCSGG 1 cut(s) 783
AsuHPI GGTGA 4 cut(s) 111, 113, 216, 245
AvaI CYCGRG 1 cut(s) 772
AvaII GGWCC 1 cut(s) 728
BaeGI GKGCMC 1 cut(s) 1107
BalI TGGCCA 1 cut(s) 668
BanI GGYRCC 1 cut(s) 365
BanII GRGCYC 1 cut(s) 779
BbsI GAAGAC 1 cut(s) 555
Bbv12I GWGCWC 1 cut(s) 1107
BbvI GCAGC 5 cut(s) 190, 586, 834, 907, 1105
BccI CCATC 5 cut(s) 34, 266, 401, 683, 1091
BciVI GTATCC 2 cut(s) 708, 779
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 1 cut(s) 783
BcoDI GTCTC 2 cut(s) 165, 826
BfaI CTAG 2 cut(s) 83, 596
BfmI CTRYAG 1 cut(s) 1108
BfuAI ACCTGC 1 cut(s) 1049
BfuI GTATCC 2 cut(s) 708, 779
BglII AGATCT 2 cut(s) 532, 978
BisI GCNGC 6 cut(s) 204, 401, 600, 848, 921, 1119
BlsI GCNGC 6 cut(s) 205, 402, 601, 849, 922, 1120
Bme1390I CCNGG 1 cut(s) 783
Bme18I GGWCC 1 cut(s) 728
BmeT110I CYCGRG 1 cut(s) 772
BmgT120I GGNCC 2 cut(s) 264, 728
BmiI GGNNCC 1 cut(s) 367
BmrFI CCNGG 1 cut(s) 783
BmsI GCATC 3 cut(s) 100, 122, 230
BpiI GAAGAC 1 cut(s) 555
Bpu10I CCTNAGC 1 cut(s) 510
BpuMI CCSGG 1 cut(s) 783
BsaJI CCNNGG 2 cut(s) 694, 799
BsaWI WCCGGW 1 cut(s) 188
Bsc4I CCNNNNNNNGG 2 cut(s) 399, 511
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 6 cut(s) 267, 454, 661, 730, 1149, 1172
Bse3DI GCAATG 2 cut(s) 222, 949
BseDI CCNNGG 2 cut(s) 694, 799
BseGI GGATG 4 cut(s) 450, 675, 762, 1102
BseLI CCNNNNNNNGG 2 cut(s) 399, 511
BseMI GCAATG 2 cut(s) 222, 949
BseMII CTCAG 2 cut(s) 314, 1176
BseNI ACTGG 6 cut(s) 267, 454, 661, 730, 1149, 1172
BseRI GAGGAG 1 cut(s) 399
BseSI GKGCMC 1 cut(s) 1107
BseXI GCAGC 5 cut(s) 190, 586, 834, 907, 1105
BseYI CCCAGC 2 cut(s) 307, 1092
BsgI GTGCAG 2 cut(s) 42, 482
Bsh1236I CGCG 1 cut(s) 484
BshFI GGCC 4 cut(s) 266, 585, 668, 1201
BshNI GGYRCC 1 cut(s) 365
BshTI ACCGGT 1 cut(s) 188
BsiHKAI GWGCWC 1 cut(s) 1107
BsiHKCI CYCGRG 1 cut(s) 772
BsiSI CCGG 2 cut(s) 189, 783
BslI CCNNNNNNNGG 2 cut(s) 399, 511
BsmAI GTCTC 2 cut(s) 165, 826
BsmI GAATGC 2 cut(s) 206, 707
BsnI GGCC 4 cut(s) 266, 585, 668, 1201
BsoBI CYCGRG 1 cut(s) 772
Bsp1286I GDGCHC 2 cut(s) 779, 1107
Bsp143I GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
BspACI CCGC 2 cut(s) 201, 400
BspANI GGCC 4 cut(s) 266, 585, 668, 1201
BspCNI CTCAG 2 cut(s) 315, 1175
BspFNI CGCG 1 cut(s) 484
BspHI TCATGA 1 cut(s) 994
BspLI GGNNCC 1 cut(s) 367
BspMI ACCTGC 1 cut(s) 1049
BspPI GGATC 4 cut(s) 522, 859, 1067, 1160
BspT107I GGYRCC 1 cut(s) 365
BsrDI GCAATG 2 cut(s) 222, 949
BsrFI RCCGGY 1 cut(s) 188
BsrI ACTGG 6 cut(s) 267, 454, 661, 730, 1149, 1172
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 2 cut(s) 694, 799
BssMI GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
Bst4CI ACNGT 4 cut(s) 233, 365, 803, 837
BstC8I GCNNGC 1 cut(s) 1103
BstDEI CTNAG 5 cut(s) 323, 510, 872, 924, 1162
BstDSI CCRYGG 1 cut(s) 799
BstF5I GGATG 4 cut(s) 450, 675, 762, 1102
BstFNI CGCG 1 cut(s) 484
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 9 cut(s) 472, 517, 535, 595, 796, 867, 981, 1075, 1168
BstMAI GTCTC 2 cut(s) 165, 826
BstMBI GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
BstMWI GCNNNNNNNGC 6 cut(s) 212, 263, 313, 374, 804, 1098
BstNSI RCATGY 4 cut(s) 16, 78, 1013, 1194
BstSCI CCNGG 1 cut(s) 781
BstSFI CTRYAG 1 cut(s) 1108
BstSLI GKGCMC 1 cut(s) 1107
BstUI CGCG 1 cut(s) 484
BstV1I GCAGC 5 cut(s) 190, 586, 834, 907, 1105
BstV2I GAAGAC 1 cut(s) 555
BstX2I RGATCY 5 cut(s) 514, 532, 978, 1072, 1165
BstYI RGATCY 5 cut(s) 514, 532, 978, 1072, 1165
BsuI GTATCC 2 cut(s) 708, 779
BsuRI GGCC 4 cut(s) 266, 585, 668, 1201
BtgI CCRYGG 1 cut(s) 799
BtsCI GGATG 4 cut(s) 450, 675, 762, 1102
BtsIMutI CAGTG 4 cut(s) 97, 274, 723, 1156
BveI ACCTGC 1 cut(s) 1049
Cac8I GCNNGC 1 cut(s) 1103
CciI TCATGA 1 cut(s) 994
CfoI GCGC 1 cut(s) 484
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 2 cut(s) 264, 728
Csp6I GTAC 3 cut(s) 6, 486, 621
CspAI ACCGGT 1 cut(s) 188
CviAII CATG 9 cut(s) 13, 75, 414, 767, 826, 995, 1010, 1065, 1191
CviQI GTAC 3 cut(s) 6, 486, 621
DdeI CTNAG 5 cut(s) 323, 510, 872, 924, 1162
DpnI GATC 9 cut(s) 471, 516, 534, 594, 795, 866, 980, 1074, 1167
DpnII GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
EaeI YGGCCR 1 cut(s) 666
Eco147I AGGCCT 1 cut(s) 1201
Eco24I GRGCYC 1 cut(s) 779
Eco47I GGWCC 1 cut(s) 728
Eco57I CTGAAG 2 cut(s) 114, 363
Eco88I CYCGRG 1 cut(s) 772
EcoRI GAATTC 1 cut(s) 178
EcoT22I ATGCAT 2 cut(s) 115, 1192
EcoT38I GRGCYC 1 cut(s) 779
FaeI CATG 9 cut(s) 16, 78, 417, 770, 829, 998, 1013, 1068, 1194
FatI CATG 9 cut(s) 12, 74, 413, 766, 825, 994, 1009, 1064, 1190
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 6 cut(s) 204, 401, 600, 848, 921, 1119
FokI GGATG 4 cut(s) 437, 662, 749, 1109
FriOI GRGCYC 1 cut(s) 779
Fsp4HI GCNGC 6 cut(s) 204, 401, 600, 848, 921, 1119
FspBI CTAG 2 cut(s) 83, 596
GlaI GCGC 1 cut(s) 483
GluI GCNGC 6 cut(s) 204, 401, 600, 848, 921, 1119
GsaI CCCAGC 2 cut(s) 311, 1096
HaeIII GGCC 4 cut(s) 266, 585, 668, 1201
HapII CCGG 2 cut(s) 189, 783
HhaI GCGC 1 cut(s) 484
Hin1II CATG 9 cut(s) 16, 78, 417, 770, 829, 998, 1013, 1068, 1194
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HindIII AAGCTT 1 cut(s) 953
HinfI GANTC 4 cut(s) 173, 338, 410, 433
HpaI GTTAAC 1 cut(s) 658
HpaII CCGG 2 cut(s) 189, 783
HphI GGTGA 4 cut(s) 111, 113, 216, 245
Hpy166II GTNNAC 2 cut(s) 658, 1105
Hpy188I TCNGA 9 cut(s) 184, 348, 359, 523, 612, 640, 680, 1143, 1165
Hpy188III TCNNGA 4 cut(s) 381, 647, 712, 995
Hpy8I GTNNAC 2 cut(s) 658, 1105
HpyAV CCTTC 4 cut(s) 132, 255, 871, 1086
HpyCH4III ACNGT 4 cut(s) 233, 365, 803, 837
HpyF10VI GCNNNNNNNGC 6 cut(s) 212, 263, 313, 374, 804, 1098
HpyF3I CTNAG 5 cut(s) 323, 510, 872, 924, 1162
Hsp92II CATG 9 cut(s) 16, 78, 417, 770, 829, 998, 1013, 1068, 1194
HspAI GCGC 1 cut(s) 482
Ksp22I TGATCA 1 cut(s) 469
KspAI GTTAAC 1 cut(s) 658
Kzo9I GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
LmnI GCTCC 2 cut(s) 348, 386
Lsp1109I GCAGC 5 cut(s) 190, 586, 834, 907, 1105
LweI GCATC 3 cut(s) 100, 122, 230
MaeI CTAG 2 cut(s) 83, 596
MaeIII GTNAC 3 cut(s) 70, 117, 748
MalI GATC 9 cut(s) 471, 516, 534, 594, 795, 866, 980, 1074, 1167
MboI GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
MboII GAAGA 7 cut(s) 116, 119, 235, 560, 988, 1010, 1082
MflI RGATCY 5 cut(s) 514, 532, 978, 1072, 1165
MhlI GDGCHC 2 cut(s) 779, 1107
MlsI TGGCCA 1 cut(s) 668
MluCI AATT 6 cut(s) 178, 342, 352, 370, 629, 642
MluNI TGGCCA 1 cut(s) 668
MlyI GAGTC 4 cut(s) 167, 332, 419, 442
MmeI TCCRAC 1 cut(s) 892
MnlI CCTC 6 cut(s) 92, 377, 689, 789, 897, 1108
Mox20I TGGCCA 1 cut(s) 668
Mph1103I ATGCAT 2 cut(s) 115, 1192
MscI TGGCCA 1 cut(s) 668
MseI TTAA 2 cut(s) 657, 1204
MslI CAYNNNNRTG 1 cut(s) 1110
Msp20I TGGCCA 1 cut(s) 668
MspA1I CMGCKG 2 cut(s) 203, 257
MspI CCGG 2 cut(s) 189, 783
MspR9I CCNGG 1 cut(s) 783
Mva1269I GAATGC 2 cut(s) 206, 707
MvnI CGCG 1 cut(s) 484
MwoI GCNNNNNNNGC 6 cut(s) 212, 263, 313, 374, 804, 1098
NciI CCSGG 1 cut(s) 783
NdeII GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
NlaIII CATG 9 cut(s) 16, 78, 417, 770, 829, 998, 1013, 1068, 1194
NlaIV GGNNCC 1 cut(s) 367
NmeAIII GCCGAG 1 cut(s) 325
NmuCI GTSAC 2 cut(s) 117, 748
NsiI ATGCAT 2 cut(s) 115, 1192
NspI RCATGY 4 cut(s) 16, 78, 1013, 1194
OliI CACNNNNGTG 1 cut(s) 1110
PagI TCATGA 1 cut(s) 994
PceI AGGCCT 1 cut(s) 1201
PciI ACATGT 3 cut(s) 12, 74, 1009
PctI GAATGC 2 cut(s) 206, 707
PfoI TCCNGGA 1 cut(s) 781
PinAI ACCGGT 1 cut(s) 188
PkrI GCNGC 6 cut(s) 205, 402, 601, 849, 922, 1120
PleI GAGTC 4 cut(s) 167, 332, 418, 441
PpsI GAGTC 4 cut(s) 167, 332, 418, 441
PscI ACATGT 3 cut(s) 12, 74, 1009
PspFI CCCAGC 2 cut(s) 307, 1092
PspN4I GGNNCC 1 cut(s) 367
PspPI GGNCC 2 cut(s) 264, 728
PsrI GAACNNNNNNTAC 2 cut(s) 458, 490
PsuI RGATCY 5 cut(s) 514, 532, 978, 1072, 1165
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 3 cut(s) 7, 487, 622
RsaNI GTAC 3 cut(s) 6, 486, 621
RseI CAYNNNNRTG 1 cut(s) 1110
SaqAI TTAA 2 cut(s) 657, 1204
SatI GCNGC 6 cut(s) 204, 401, 600, 848, 921, 1119
Sau3AI GATC 9 cut(s) 469, 514, 532, 592, 793, 864, 978, 1072, 1165
Sau96I GGNCC 2 cut(s) 264, 728
SchI GAGTC 4 cut(s) 167, 332, 419, 442
ScrFI CCNGG 1 cut(s) 783
SduI GDGCHC 2 cut(s) 779, 1107
SfaNI GCATC 3 cut(s) 100, 122, 230
SfcI CTRYAG 1 cut(s) 1108
SinI GGWCC 1 cut(s) 728
SmiMI CAYNNNNRTG 1 cut(s) 1110
Sse9I AATT 6 cut(s) 178, 342, 352, 370, 629, 642
SseBI AGGCCT 1 cut(s) 1201
SsiI CCGC 2 cut(s) 201, 400
SspMI CTAG 2 cut(s) 83, 596
StuI AGGCCT 1 cut(s) 1201
StyD4I CCNGG 1 cut(s) 781
TaaI ACNGT 4 cut(s) 233, 365, 803, 837
TaqI TCGA 3 cut(s) 176, 576, 966
TaqII GACCGA 1 cut(s) 708
TasI AATT 6 cut(s) 178, 342, 352, 370, 629, 642
TatI WGTACW 1 cut(s) 5
TauI GCSGC 1 cut(s) 403
Tru1I TTAA 2 cut(s) 657, 1204
Tru9I TTAA 2 cut(s) 657, 1204
TscAI CASTG 4 cut(s) 97, 274, 730, 1156
TseFI GTSAC 2 cut(s) 117, 748
TseI GCWGC 5 cut(s) 203, 599, 847, 920, 1118
Tsp45I GTSAC 2 cut(s) 117, 748
TspDTI ATGAA 6 cut(s) 318, 983, 1011, 1020, 1053, 1146
TspRI CASTG 4 cut(s) 97, 274, 730, 1156
VneI GTGCAC 1 cut(s) 1103
VpaK11BI GGWCC 1 cut(s) 728
XapI RAATTY 2 cut(s) 178, 642
XceI RCATGY 4 cut(s) 16, 78, 1013, 1194
XcmI CCANNNNNNNNNTGG 1 cut(s) 254
XspI CTAG 2 cut(s) 83, 596
Zsp2I ATGCAT 2 cut(s) 115, 1192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.