AT5G55050

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002678
Physical Location & Seq
Forward (+)
22337626 .. 22340389
2764 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G55050.1

Sequence Viewer

Length: 1131 bp
ATGCCGACGAACAACACTCCGTTCCTCACTATTTTTCTCCTCTTTCTTGGTTTACTCCGGTTCGATTCATTTCCCGGTTTAGAAGCTGCAACCGGGAAATTAGCTTCGATTCCAGGATTATACGTGTTCGGAGATTCGTTAGTTGATGCCGGCAACAATAATTACTTACCAATTTCAATATCTAAAGCTAATTATCCACATAACGGCGTCGATTTTCCGAACAAGAAACCTACCGGAAGATTCTGTAACGGCAAGAACGCCGCCGATGCTATCGCTGAGAAATTTGGTTTACCGTTACCACCTCCCTATCTCTCACTTAGAGGCTTACTAAAAAGGGAGAAGAGAAAATCTGCCGCCGTGACTGGTGTGAATTTTGCTTCCGGTGGAGCCGGAATCTTCAACAGTTCCGACCAAAAACTTGGACAAGCTATTCCTTTATCCAAGCAAGTCAACAATTGGCTCTCTATTCATGAAGAAGTCATGAAACTTGAACCATCCGCAGCACAGCTTCACCTATCCAAATCTCTATTCACTGTTGTCATAGGAAGTAACGATCTTTTCGATTACTTTGGATCGTTCAAACTCCGACGCCAGAGCAATCCTCAACAATATACACAATTAATGGCTGATAAACTCAAAGAGCAATTGAAGAGAATTCACGATAGTGGAGCGCGTAGATTCCTTATCATCGGGGTAGCACAAATTGGTTGCACACCGGGAAAAAGAGCGAAGAACTCGACACTCCATGAATGTGACGAAGGGGCAAACATGTGGTGCTCTTTGTACAACGAAGCTCTAGTAAAGATGCTGCAACAACTCAAACAAGAATTACAAGGCTCAATAACTTACACTTACTTTGATAACTACAAGTCCTTACATGACATTATTTCCAACCCTGCCCGTTACGGTTTTGCTGACGTTACATCTGCGTGTTGTGGAAATGGGGAATTGAACGCAGACCTTCCTTGTCTGCCTTTGGCGAAGTTATGTTCAGACAGAACCAAACATCTTTTTTGGGATCGCTATGGCCATCCCACTGAAGCTGCTGCTCGGACCATCGTCGATCTTATGTTGACTGATGATACACATTACTCATCTCCTATAACTCTTACTCAGTTGGTCTCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

376

Amino Acids

41.45

Weight (kDa)

8.77

Isoelectric Point (pI)

33.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 40 - 355 3e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 673
AciI CCGC 3 cut(s) 261, 354, 498
AclWI GGATC 2 cut(s) 580, 1026
AcoI YGGCCR 1 cut(s) 1027
AcsI RAATTY 3 cut(s) 281, 370, 654
AcuI CTGAAG 1 cut(s) 1059
AcyI GRCGYC 2 cut(s) 207, 589
AfaI GTAC 1 cut(s) 785
AfiI CCNNNNNNNGG 1 cut(s) 203
AflIII ACRYGT 2 cut(s) 123, 768
AgsI TTSAA 6 cut(s) 177, 400, 491, 580, 649, 952
AjnI CCWGG 1 cut(s) 112
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
AleI CACNNNNGTG 1 cut(s) 663
AluBI AGCT 7 cut(s) 86, 104, 188, 428, 508, 794, 1043
AluI AGCT 7 cut(s) 86, 104, 188, 428, 508, 794, 1043
Alw21I GWGCWC 1 cut(s) 779
Alw26I GTCTC 1 cut(s) 1126
AlwI GGATC 2 cut(s) 580, 1026
AoxI GGCC 1 cut(s) 1027
ApeKI GCWGC 5 cut(s) 86, 500, 808, 1043, 1046
ApoI RAATTY 3 cut(s) 281, 370, 654
AseI ATTAAT 1 cut(s) 620
AspLEI GCGC 1 cut(s) 673
AspS9I GGNCC 1 cut(s) 1053
AsuC2I CCSGG 3 cut(s) 75, 94, 717
AsuHPI GGTGA 1 cut(s) 503
AvaII GGWCC 1 cut(s) 1053
BalI TGGCCA 1 cut(s) 1029
Bbv12I GWGCWC 1 cut(s) 779
BbvI GCAGC 5 cut(s) 73, 512, 795, 1030, 1033
BccI CCATC 3 cut(s) 502, 1038, 1064
BceAI ACGGC 3 cut(s) 220, 265, 341
BciT130I CCWGG 1 cut(s) 114
BcnI CCSGG 3 cut(s) 75, 94, 717
BcoDI GTCTC 1 cut(s) 1126
BfaI CTAG 1 cut(s) 797
BisI GCNGC 7 cut(s) 87, 261, 354, 501, 809, 1044, 1047
BlsI GCNGC 7 cut(s) 88, 262, 355, 502, 810, 1045, 1048
Bme1390I CCNGG 4 cut(s) 75, 94, 114, 717
Bme18I GGWCC 1 cut(s) 1053
BmgT120I GGNCC 1 cut(s) 1053
BmiI GGNNCC 1 cut(s) 388
BmrFI CCNGG 4 cut(s) 75, 94, 114, 717
BmsI GCATC 3 cut(s) 136, 256, 795
BoxI GACNNNNGTC 1 cut(s) 1058
BplI GAGNNNNNCTC 2 cut(s) 586, 618
BpuMI CCSGG 3 cut(s) 75, 94, 717
BsaAI YACGTR 1 cut(s) 124
BsaHI GRCGYC 2 cut(s) 207, 589
BsaI GGTCTC 1 cut(s) 1126
BsaWI WCCGGW 3 cut(s) 57, 233, 380
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 367
BseBI CCWGG 1 cut(s) 114
BseGI GGATG 2 cut(s) 494, 1030
BseLI CCNNNNNNNGG 1 cut(s) 203
BseMII CTCAG 2 cut(s) 267, 1127
BseNI ACTGG 1 cut(s) 367
BseRI GAGGAG 1 cut(s) 29
BseXI GCAGC 5 cut(s) 73, 512, 795, 1030, 1033
Bsh1236I CGCG 1 cut(s) 673
BshFI GGCC 1 cut(s) 1029
BsiHKAI GWGCWC 1 cut(s) 779
BsiSI CCGG 8 cut(s) 58, 75, 93, 150, 234, 381, 390, 716
BslI CCNNNNNNNGG 1 cut(s) 203
BsmAI GTCTC 1 cut(s) 1126
BsnI GGCC 1 cut(s) 1029
Bso31I GGTCTC 1 cut(s) 1126
Bsp1286I GDGCHC 1 cut(s) 779
Bsp1407I TGTACA 1 cut(s) 783
Bsp143I GATC 4 cut(s) 553, 572, 1018, 1063
BspACI CCGC 3 cut(s) 261, 354, 498
BspANI GGCC 1 cut(s) 1029
BspCNI CTCAG 2 cut(s) 268, 1126
BspFNI CGCG 1 cut(s) 673
BspHI TCATGA 2 cut(s) 469, 480
BspLI GGNNCC 1 cut(s) 388
BspPI GGATC 2 cut(s) 580, 1026
BspTNI GGTCTC 1 cut(s) 1126
BsrFI RCCGGY 1 cut(s) 149
BsrGI TGTACA 1 cut(s) 783
BsrI ACTGG 1 cut(s) 367
BssAI RCCGGY 1 cut(s) 149
BssMI GATC 4 cut(s) 553, 572, 1018, 1063
BssNI GRCGYC 2 cut(s) 207, 589
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 4 cut(s) 294, 404, 535, 908
Bst6I CTCTTC 2 cut(s) 335, 644
BstACI GRCGYC 2 cut(s) 207, 589
BstAUI TGTACA 1 cut(s) 783
BstBAI YACGTR 1 cut(s) 124
BstC8I GCNNGC 1 cut(s) 151
BstDEI CTNAG 3 cut(s) 276, 317, 1113
BstF5I GGATG 2 cut(s) 494, 1030
BstFNI CGCG 1 cut(s) 673
BstHHI GCGC 1 cut(s) 673
BstKTI GATC 4 cut(s) 556, 575, 1021, 1066
BstMAI GTCTC 1 cut(s) 1126
BstMBI GATC 4 cut(s) 553, 572, 1018, 1063
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstNI CCWGG 1 cut(s) 114
BstNSI RCATGY 1 cut(s) 772
BstPAI GACNNNNGTC 1 cut(s) 1058
BstSCI CCNGG 4 cut(s) 73, 92, 112, 715
BstUI CGCG 1 cut(s) 673
BstV1I GCAGC 5 cut(s) 73, 512, 795, 1030, 1033
BstXI CCANNNNNNTGG 1 cut(s) 419
BsuRI GGCC 1 cut(s) 1029
BtsCI GGATG 2 cut(s) 494, 1030
BtsIMutI CAGTG 2 cut(s) 531, 1035
Cac8I GCNNGC 1 cut(s) 151
CciI TCATGA 2 cut(s) 469, 480
CfoI GCGC 1 cut(s) 673
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 1053
CseI GACGC 2 cut(s) 196, 597
Csp6I GTAC 1 cut(s) 784
CviAII CATG 6 cut(s) 470, 481, 746, 769, 878, 1128
CviQI GTAC 1 cut(s) 784
DdeI CTNAG 3 cut(s) 276, 317, 1113
DpnI GATC 4 cut(s) 555, 574, 1020, 1065
DpnII GATC 4 cut(s) 553, 572, 1018, 1063
EaeI YGGCCR 1 cut(s) 1027
Eam1104I CTCTTC 2 cut(s) 335, 644
EarI CTCTTC 2 cut(s) 335, 644
Eco31I GGTCTC 1 cut(s) 1126
Eco47I GGWCC 1 cut(s) 1053
Eco57I CTGAAG 1 cut(s) 1059
EcoRI GAATTC 1 cut(s) 654
EcoRII CCWGG 1 cut(s) 112
FaeI CATG 6 cut(s) 473, 484, 749, 772, 881, 1131
FatI CATG 6 cut(s) 469, 480, 745, 768, 877, 1127
Fnu4HI GCNGC 7 cut(s) 87, 261, 354, 501, 809, 1044, 1047
FokI GGATG 2 cut(s) 481, 1017
Fsp4HI GCNGC 7 cut(s) 87, 261, 354, 501, 809, 1044, 1047
FspBI CTAG 1 cut(s) 797
GlaI GCGC 1 cut(s) 672
GluI GCNGC 7 cut(s) 87, 261, 354, 501, 809, 1044, 1047
HaeIII GGCC 1 cut(s) 1029
HapII CCGG 8 cut(s) 58, 75, 93, 150, 234, 381, 390, 716
HgaI GACGC 2 cut(s) 196, 597
HhaI GCGC 1 cut(s) 673
Hin1I GRCGYC 2 cut(s) 207, 589
Hin1II CATG 6 cut(s) 473, 484, 749, 772, 881, 1131
Hin6I GCGC 1 cut(s) 671
HinP1I GCGC 1 cut(s) 671
HincII GTYRAC 2 cut(s) 451, 1074
HindII GTYRAC 2 cut(s) 451, 1074
HinfI GANTC 6 cut(s) 65, 109, 134, 240, 393, 678
HpaII CCGG 8 cut(s) 58, 75, 93, 150, 234, 381, 390, 716
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 4 cut(s) 53, 290, 451, 1074
Hpy188I TCNGA 6 cut(s) 131, 219, 409, 587, 994, 1053
Hpy188III TCNNGA 3 cut(s) 470, 481, 659
Hpy8I GTNNAC 4 cut(s) 53, 290, 451, 1074
Hpy99I CGWCG 4 cut(s) 10, 212, 591, 1064
HpyAV CCTTC 2 cut(s) 752, 971
HpyCH4III ACNGT 4 cut(s) 294, 404, 535, 908
HpyCH4IV ACGT 2 cut(s) 123, 918
HpyCH4V TGCA 3 cut(s) 89, 711, 811
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
HpyF3I CTNAG 3 cut(s) 276, 317, 1113
HpySE526I ACGT 2 cut(s) 123, 918
Hsp92I GRCGYC 2 cut(s) 207, 589
Hsp92II CATG 6 cut(s) 473, 484, 749, 772, 881, 1131
HspAI GCGC 1 cut(s) 671
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
Kzo9I GATC 4 cut(s) 553, 572, 1018, 1063
LmnI GCTCC 2 cut(s) 386, 668
Lsp1109I GCAGC 5 cut(s) 73, 512, 795, 1030, 1033
LweI GCATC 3 cut(s) 136, 256, 795
MaeI CTAG 1 cut(s) 797
MaeII ACGT 2 cut(s) 123, 918
MaeIII GTNAC 7 cut(s) 245, 294, 358, 548, 752, 902, 919
MalI GATC 4 cut(s) 555, 574, 1020, 1065
MboI GATC 4 cut(s) 553, 572, 1018, 1063
MboII GAAGA 6 cut(s) 249, 352, 388, 485, 661, 742
MfeI CAATTG 2 cut(s) 454, 644
MhlI GDGCHC 1 cut(s) 779
MlsI TGGCCA 1 cut(s) 1029
MluNI TGGCCA 1 cut(s) 1029
MmeI TCCRAC 3 cut(s) 432, 610, 915
MnlI CCTC 5 cut(s) 35, 50, 312, 314, 612
Mox20I TGGCCA 1 cut(s) 1029
MroNI GCCGGC 1 cut(s) 149
MscI TGGCCA 1 cut(s) 1029
MseI TTAA 1 cut(s) 620
MslI CAYNNNNRTG 3 cut(s) 663, 750, 928
Msp20I TGGCCA 1 cut(s) 1029
MspI CCGG 8 cut(s) 58, 75, 93, 150, 234, 381, 390, 716
MspR9I CCNGG 4 cut(s) 75, 94, 114, 717
MunI CAATTG 2 cut(s) 454, 644
MvaI CCWGG 1 cut(s) 114
MvnI CGCG 1 cut(s) 673
MwoI GCNNNNNNNGC 1 cut(s) 266
NaeI GCCGGC 1 cut(s) 151
NciI CCSGG 3 cut(s) 75, 94, 717
NdeII GATC 4 cut(s) 553, 572, 1018, 1063
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 6 cut(s) 473, 484, 749, 772, 881, 1131
NlaIV GGNNCC 1 cut(s) 388
NmuCI GTSAC 2 cut(s) 358, 752
NspI RCATGY 1 cut(s) 772
OliI CACNNNNGTG 1 cut(s) 663
PagI TCATGA 2 cut(s) 469, 480
PciI ACATGT 1 cut(s) 768
PcsI WCGNNNNNNNCGW 1 cut(s) 558
PdiI GCCGGC 1 cut(s) 151
PfeI GAWTC 6 cut(s) 65, 109, 134, 240, 393, 678
PfoI TCCNGGA 1 cut(s) 112
PkrI GCNGC 7 cut(s) 88, 262, 355, 502, 810, 1045, 1048
Ppu21I YACGTR 1 cut(s) 124
PscI ACATGT 1 cut(s) 768
PshAI GACNNNNGTC 1 cut(s) 1058
PshBI ATTAAT 1 cut(s) 620
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 1 cut(s) 388
PspPI GGNCC 1 cut(s) 1053
RsaI GTAC 1 cut(s) 785
RsaNI GTAC 1 cut(s) 784
RseI CAYNNNNRTG 3 cut(s) 663, 750, 928
SaqAI TTAA 1 cut(s) 620
SatI GCNGC 7 cut(s) 87, 261, 354, 501, 809, 1044, 1047
Sau3AI GATC 4 cut(s) 553, 572, 1018, 1063
Sau96I GGNCC 1 cut(s) 1053
ScrFI CCNGG 4 cut(s) 75, 94, 114, 717
SduI GDGCHC 1 cut(s) 779
SfaNI GCATC 3 cut(s) 136, 256, 795
SinI GGWCC 1 cut(s) 1053
SmiMI CAYNNNNRTG 3 cut(s) 663, 750, 928
SsiI CCGC 3 cut(s) 261, 354, 498
SspMI CTAG 1 cut(s) 797
StyD4I CCNGG 4 cut(s) 73, 92, 112, 715
TaaI ACNGT 4 cut(s) 294, 404, 535, 908
TaiI ACGT 2 cut(s) 126, 921
TaqI TCGA 6 cut(s) 63, 107, 210, 561, 737, 1062
TatI WGTACW 1 cut(s) 783
TauI GCSGC 2 cut(s) 263, 356
TfiI GAWTC 6 cut(s) 65, 109, 134, 240, 393, 678
Tru1I TTAA 1 cut(s) 620
Tru9I TTAA 1 cut(s) 620
TscAI CASTG 2 cut(s) 538, 1042
TseFI GTSAC 2 cut(s) 358, 752
TseI GCWGC 5 cut(s) 86, 500, 808, 1043, 1046
Tsp45I GTSAC 2 cut(s) 358, 752
TspDTI ATGAA 5 cut(s) 57, 458, 486, 497, 762
TspGWI ACGGA 1 cut(s) 9
TspRI CASTG 2 cut(s) 538, 1042
VpaK11BI GGWCC 1 cut(s) 1053
VspI ATTAAT 1 cut(s) 620
XapI RAATTY 3 cut(s) 281, 370, 654
XceI RCATGY 1 cut(s) 772
XspI CTAG 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.