Rw5G020920

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002678
Physical Location & Seq
Forward (+)
27772468 .. 27775246
2779 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G020920.1

Sequence Viewer

Length: 1092 bp
ATGGCCTGCAATTCCTTCCTTCCAAGTTTCTGCGTCTTCTTGCTTTTCTTGCTGTACGGGGTTTCGTATACAGTTACGGAGGCTCGTGTGATTCCGGCCATGTTTGTTTTCGGAGACTCCCTAGTAGATGTGGGCAACAACAATTACCTCAGGTATTCATTTGCCAAAGCAAACTTTCCTCACAATGGGGTCGACTTTCCGACCAGAAAACCAACCGGCAGGTTCGGCAATGGCAAGAATGCTGCAGATCTTATTGCTGAGAGAATGGGGTTGCCGACAATACCACCGTATCTCTCCATGTCATCCAAATCAAACAAGAGCATTACACAGTTCCTAAACGGCGTTAACTTTGCATCTGGAGCTTCCAAAATCTTAAACGACATAGATCCACAATATCCCTTTTCCATACCTTTGGAAAAACAAGTAGACTACTATTTGGCAGTGCATAAAGACCTAGCGCAGGGGCTAGGAGCCTCCAGAGCACAAATTTATTTATCAAAATCTCTCTTCCTCATTATCACGGGAAGCAATGACATCTACAACTACTTTGACTCGTTAAGTAACAGCACACAACAGCAGTACGTGAACTCCATTGTTCTCATCTTCAAAGAACAAGTGAAGCGGCTGTATGATTATGGTGCACGCAAATTCTCGATTGTTGGGGTTGGGGTTATCGGATGCACACCATCAGAAAGGAATGAGCAGGCGGACAGAAAATGCAATGAAGATACAAATCGATTGTCACTCAAGTACAATCAAGCACTCGTGTCCATGTTGAAGAATTTGGCATCAGAGCTCAGAGGCATAAACTACTCCTACTTTGATGGTTACAGCGTCATGCAGAACTTCATCCAAAAACCAACAGCTTATGGATTTTCTGAGGTTAAAGCTGCTTGCTGTGGTCTTGGGAATCTCAATGCCGATGCTCCTTGCCTGCCATTTGCGACTTACTGCACCTACAGAAGCAACCATCTGTTCTGGGACAGAACGCATCCTACTGAGGCAGCTCATCGCAAGCTTGTGGATTATATGCTTTATGGTCCTTTACAATACACATTTCCACTCAATGTGAAAAAGCTGGTTGCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

363

Amino Acids

40.68

Weight (kDa)

8.92

Isoelectric Point (pI)

24.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 343 6.6e-36 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 210
AccI GTMKAC 3 cut(s) 68, 192, 426
AciI CCGC 2 cut(s) 622, 707
AclWI GGATC 1 cut(s) 380
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 3 cut(s) 486, 647, 781
AfaI GTAC 3 cut(s) 56, 581, 752
AfiI CCNNNNNNNGG 2 cut(s) 185, 460
AgsI TTSAA 2 cut(s) 607, 778
AluBI AGCT 7 cut(s) 362, 796, 866, 890, 1007, 1018, 1078
AluI AGCT 7 cut(s) 362, 796, 866, 890, 1007, 1018, 1078
Alw21I GWGCWC 3 cut(s) 484, 643, 798
Alw26I GTCTC 1 cut(s) 108
Alw44I GTGCAC 1 cut(s) 639
AlwI GGATC 1 cut(s) 380
AoxI GGCC 2 cut(s) 3, 96
ApaLI GTGCAC 1 cut(s) 639
ApeKI GCWGC 3 cut(s) 242, 890, 1004
ApoI RAATTY 3 cut(s) 486, 647, 781
ArsI GACNNNNNNTTYG 2 cut(s) 725, 757
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 1 cut(s) 1040
AvaII GGWCC 1 cut(s) 1040
AxyI CCTNAGG 1 cut(s) 149
BaeGI GKGCMC 1 cut(s) 643
BaeI ACNNNNGTAYC 2 cut(s) 272, 305
BanII GRGCYC 1 cut(s) 798
BauI CACGAG 2 cut(s) 84, 764
BbsI GAAGAC 1 cut(s) 28
Bbv12I GWGCWC 3 cut(s) 484, 643, 798
BbvI GCAGC 3 cut(s) 229, 877, 1016
BccI CCATC 3 cut(s) 694, 818, 978
BceAI ACGGC 1 cut(s) 355
BcoDI GTCTC 1 cut(s) 108
BfaI CTAG 3 cut(s) 122, 455, 467
BfmI CTRYAG 2 cut(s) 243, 958
BfuAI ACCTGC 1 cut(s) 210
BglII AGATCT 1 cut(s) 247
BisI GCNGC 4 cut(s) 243, 623, 891, 1005
BlsI GCNGC 4 cut(s) 244, 624, 892, 1006
Bme18I GGWCC 1 cut(s) 1040
BmgT120I GGNCC 1 cut(s) 1040
BmiI GGNNCC 1 cut(s) 472
BmsI GCATC 5 cut(s) 362, 668, 797, 913, 1000
BpiI GAAGAC 1 cut(s) 28
BpmI CTGGAG 2 cut(s) 378, 460
BpuEI CTTGAG 1 cut(s) 731
Bsa29I ATCGAT 1 cut(s) 736
BsaAI YACGTR 1 cut(s) 583
BsaBI GATNNNNATC 1 cut(s) 732
BsaXI ACNNNNNCTCC 2 cut(s) 572, 602
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 460
Bse118I RCCGGY 1 cut(s) 215
Bse21I CCTNAGG 1 cut(s) 149
Bse3DI GCAATG 3 cut(s) 235, 535, 727
Bse8I GATNNNNATC 1 cut(s) 732
BseCI ATCGAT 1 cut(s) 736
BseGI GGATG 4 cut(s) 302, 683, 849, 991
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 2 cut(s) 185, 460
BseMI GCAATG 3 cut(s) 235, 535, 727
BseMII CTCAG 5 cut(s) 163, 249, 811, 870, 990
BseSI GKGCMC 1 cut(s) 643
BseXI GCAGC 3 cut(s) 229, 877, 1016
BsgI GTGCAG 1 cut(s) 937
BshFI GGCC 2 cut(s) 5, 98
BshVI ATCGAT 1 cut(s) 736
BsiHKAI GWGCWC 3 cut(s) 484, 643, 798
BsiSI CCGG 2 cut(s) 95, 216
BslFI GGGAC 1 cut(s) 995
BslI CCNNNNNNNGG 2 cut(s) 185, 460
BsmAI GTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 995
BsmI GAATGC 1 cut(s) 244
BsnI GGCC 2 cut(s) 5, 98
Bsp1286I GDGCHC 3 cut(s) 484, 643, 798
Bsp143I GATC 2 cut(s) 247, 385
BspACI CCGC 2 cut(s) 622, 707
BspANI GGCC 2 cut(s) 5, 98
BspCNI CTCAG 5 cut(s) 162, 250, 810, 871, 991
BspDI ATCGAT 1 cut(s) 736
BspLI GGNNCC 1 cut(s) 472
BspMAI CTGCAG 1 cut(s) 247
BspMI ACCTGC 1 cut(s) 210
BspPI GGATC 1 cut(s) 380
BsrDI GCAATG 3 cut(s) 235, 535, 727
BsrFI RCCGGY 1 cut(s) 215
BssAI RCCGGY 1 cut(s) 215
BssMI GATC 2 cut(s) 247, 385
BssNAI GTATAC 1 cut(s) 69
BssSI CACGAG 2 cut(s) 84, 764
Bst1107I GTATAC 1 cut(s) 69
Bst2BI CACGAG 2 cut(s) 84, 764
Bst4CI ACNGT 3 cut(s) 73, 288, 330
Bst6I CTCTTC 1 cut(s) 512
BstBAI YACGTR 1 cut(s) 583
BstC8I GCNNGC 6 cut(s) 7, 643, 705, 895, 935, 1016
BstDEI CTNAG 5 cut(s) 149, 258, 797, 879, 999
BstENI CCTNNNNNAGG 1 cut(s) 458
BstF5I GGATG 4 cut(s) 302, 683, 849, 991
BstHHI GCGC 1 cut(s) 460
BstKTI GATC 2 cut(s) 250, 388
BstMAI GTCTC 1 cut(s) 108
BstMBI GATC 2 cut(s) 247, 385
BstMWI GCNNNNNNNGC 4 cut(s) 49, 225, 359, 479
BstSFI CTRYAG 2 cut(s) 243, 958
BstSLI GKGCMC 1 cut(s) 643
BstV1I GCAGC 3 cut(s) 229, 877, 1016
BstV2I GAAGAC 1 cut(s) 28
BstX2I RGATCY 2 cut(s) 247, 385
BstXI CCANNNNNNTGG 1 cut(s) 412
BstYI RGATCY 2 cut(s) 247, 385
BstZ17I GTATAC 1 cut(s) 69
Bsu15I ATCGAT 1 cut(s) 736
Bsu36I CCTNAGG 1 cut(s) 149
BsuRI GGCC 2 cut(s) 5, 98
BsuTUI ATCGAT 1 cut(s) 736
BtgZI GCGATG 1 cut(s) 995
BtsCI GGATG 4 cut(s) 302, 683, 849, 991
BtsI GCAGTG 1 cut(s) 447
BtsIMutI CAGTG 1 cut(s) 447
BveI ACCTGC 1 cut(s) 210
Cac8I GCNNGC 6 cut(s) 7, 643, 705, 895, 935, 1016
CfoI GCGC 1 cut(s) 460
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 1040
ClaI ATCGAT 1 cut(s) 736
CseI GACGC 2 cut(s) 22, 823
Csp6I GTAC 3 cut(s) 55, 580, 751
CviAII CATG 4 cut(s) 100, 298, 772, 838
CviQI GTAC 3 cut(s) 55, 580, 751
DdeI CTNAG 5 cut(s) 149, 258, 797, 879, 999
DpnI GATC 2 cut(s) 249, 387
DpnII GATC 2 cut(s) 247, 385
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 1 cut(s) 512
EarI CTCTTC 1 cut(s) 512
EciI GGCGGA 1 cut(s) 722
Ecl136II GAGCTC 1 cut(s) 796
Eco24I GRGCYC 1 cut(s) 798
Eco47I GGWCC 1 cut(s) 1040
Eco53kI GAGCTC 1 cut(s) 796
Eco81I CCTNAGG 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 796
EcoNI CCTNNNNNAGG 1 cut(s) 458
EcoT38I GRGCYC 1 cut(s) 798
FaeI CATG 4 cut(s) 103, 301, 775, 841
FaqI GGGAC 1 cut(s) 995
FatI CATG 4 cut(s) 99, 297, 771, 837
FblI GTMKAC 3 cut(s) 68, 192, 426
Fnu4HI GCNGC 4 cut(s) 243, 623, 891, 1005
FokI GGATG 4 cut(s) 289, 690, 836, 978
FriOI GRGCYC 1 cut(s) 798
Fsp4HI GCNGC 4 cut(s) 243, 623, 891, 1005
FspBI CTAG 3 cut(s) 122, 455, 467
GlaI GCGC 1 cut(s) 459
GluI GCNGC 4 cut(s) 243, 623, 891, 1005
GsuI CTGGAG 2 cut(s) 378, 460
HaeIII GGCC 2 cut(s) 5, 98
HapII CCGG 2 cut(s) 95, 216
HgaI GACGC 2 cut(s) 22, 823
HhaI GCGC 1 cut(s) 460
Hin1II CATG 4 cut(s) 103, 301, 775, 841
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HincII GTYRAC 2 cut(s) 193, 346
HindII GTYRAC 2 cut(s) 193, 346
HindIII AAGCTT 1 cut(s) 1016
HinfI GANTC 4 cut(s) 91, 116, 551, 910
HpaI GTTAAC 1 cut(s) 346
HpaII CCGG 2 cut(s) 95, 216
Hpy166II GTNNAC 6 cut(s) 69, 193, 346, 427, 586, 641
Hpy188I TCNGA 7 cut(s) 113, 201, 677, 691, 793, 800, 880
Hpy188III TCNNGA 3 cut(s) 357, 477, 652
Hpy8I GTNNAC 6 cut(s) 69, 193, 346, 427, 586, 641
HpyAV CCTTC 2 cut(s) 25, 29
HpyCH4III ACNGT 3 cut(s) 73, 288, 330
HpyCH4IV ACGT 1 cut(s) 582
HpyCH4V TGCA 9 cut(s) 9, 245, 353, 445, 641, 681, 720, 841, 954
HpyF10VI GCNNNNNNNGC 4 cut(s) 49, 225, 359, 479
HpyF3I CTNAG 5 cut(s) 149, 258, 797, 879, 999
HpySE526I ACGT 1 cut(s) 582
Hsp92II CATG 4 cut(s) 103, 301, 775, 841
HspAI GCGC 1 cut(s) 458
KspAI GTTAAC 1 cut(s) 346
Kzo9I GATC 2 cut(s) 247, 385
LmnI GCTCC 3 cut(s) 359, 470, 931
Lsp1109I GCAGC 3 cut(s) 229, 877, 1016
LweI GCATC 5 cut(s) 362, 668, 797, 913, 1000
MaeI CTAG 3 cut(s) 122, 455, 467
MaeII ACGT 1 cut(s) 582
MaeIII GTNAC 4 cut(s) 73, 560, 741, 827
MalI GATC 2 cut(s) 249, 387
MboI GATC 2 cut(s) 247, 385
MboII GAAGA 5 cut(s) 28, 499, 595, 737, 790
MflI RGATCY 2 cut(s) 247, 385
MhlI GDGCHC 3 cut(s) 484, 643, 798
MluCI AATT 5 cut(s) 10, 142, 486, 647, 781
MlyI GAGTC 2 cut(s) 110, 545
MmeI TCCRAC 1 cut(s) 224
MnlI CCTC 8 cut(s) 73, 158, 189, 484, 521, 794, 874, 994
MseI TTAA 4 cut(s) 345, 374, 557, 885
MspI CCGG 2 cut(s) 95, 216
Mva1269I GAATGC 1 cut(s) 244
MwoI GCNNNNNNNGC 4 cut(s) 49, 225, 359, 479
NdeII GATC 2 cut(s) 247, 385
NlaIII CATG 4 cut(s) 103, 301, 775, 841
NlaIV GGNNCC 1 cut(s) 472
NmuCI GTSAC 1 cut(s) 741
PctI GAATGC 1 cut(s) 244
PfeI GAWTC 2 cut(s) 91, 910
PkrI GCNGC 4 cut(s) 244, 624, 892, 1006
PleI GAGTC 2 cut(s) 110, 545
PpsI GAGTC 2 cut(s) 110, 545
Ppu21I YACGTR 1 cut(s) 583
Psp124BI GAGCTC 1 cut(s) 798
PspN4I GGNNCC 1 cut(s) 472
PspPI GGNCC 1 cut(s) 1040
PsrI GAACNNNNNNTAC 2 cut(s) 979, 1011
PstI CTGCAG 1 cut(s) 247
PsuI RGATCY 2 cut(s) 247, 385
RsaI GTAC 3 cut(s) 56, 581, 752
RsaNI GTAC 3 cut(s) 55, 580, 751
SacI GAGCTC 1 cut(s) 798
SalI GTCGAC 1 cut(s) 191
SaqAI TTAA 4 cut(s) 345, 374, 557, 885
SatI GCNGC 4 cut(s) 243, 623, 891, 1005
Sau3AI GATC 2 cut(s) 247, 385
Sau96I GGNCC 1 cut(s) 1040
SchI GAGTC 2 cut(s) 110, 545
SduI GDGCHC 3 cut(s) 484, 643, 798
SfaNI GCATC 5 cut(s) 362, 668, 797, 913, 1000
SfcI CTRYAG 2 cut(s) 243, 958
SinI GGWCC 1 cut(s) 1040
SmlI CTYRAG 1 cut(s) 746
SmoI CTYRAG 1 cut(s) 746
Sse9I AATT 5 cut(s) 10, 142, 486, 647, 781
SsiI CCGC 2 cut(s) 622, 707
SspMI CTAG 3 cut(s) 122, 455, 467
SstI GAGCTC 1 cut(s) 798
TaaI ACNGT 3 cut(s) 73, 288, 330
TaiI ACGT 1 cut(s) 585
TaqI TCGA 3 cut(s) 192, 653, 736
TasI AATT 5 cut(s) 10, 142, 486, 647, 781
TatI WGTACW 1 cut(s) 750
TauI GCSGC 1 cut(s) 625
TfiI GAWTC 2 cut(s) 91, 910
Tru1I TTAA 4 cut(s) 345, 374, 557, 885
Tru9I TTAA 4 cut(s) 345, 374, 557, 885
TscAI CASTG 1 cut(s) 447
TseFI GTSAC 1 cut(s) 741
TseI GCWGC 3 cut(s) 242, 890, 1004
Tsp45I GTSAC 1 cut(s) 741
TspDTI ATGAA 3 cut(s) 147, 738, 838
TspGWI ACGGA 1 cut(s) 92
TspRI CASTG 1 cut(s) 447
VneI GTGCAC 1 cut(s) 639
VpaK11BI GGWCC 1 cut(s) 1040
XagI CCTNNNNNAGG 1 cut(s) 458
XapI RAATTY 3 cut(s) 486, 647, 781
XmiI GTMKAC 3 cut(s) 68, 192, 426
XspI CTAG 3 cut(s) 122, 455, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.