MD11G1215100.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002678
Physical Location & Seq
Reverse (-)
31406692 .. 31409413
2722 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1215100.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGGCTAAATATCACACTGATGTTTTGCCAATGGCTTCCTTCGTTGTCTTCTCTCTCTTCATTACTAGTTTCATCGTCACTCTCTCGGAAGCTCAGATGGTTCCGGCCATTTACGTGTTCGGAGACTCATTAGCCGATGTTGGTAACAACAATTACCTAAAAATTTCTCTAGTCAAGGCAAATTTTCCTCATAATGGGGTTGATTATCCTGACAAAGTAGCAACGGGGAGGTTTGGCAATGGCAAGAATTCTGCAGATTTTCTTGCCGAGAAAATAGGGTTACTACCGTCGCCACCATATCTGGCACTCGTATCCAAGTCCAATAGGCGAAAAATACTATCCTTCGTCGACGGAGTTAGCTTCGCCTCCGGAGGAGCTGGAATCTTCAATGGCTCGGATGAACAATTTCGTCAGTCAATACCTTTGTCATACCAAGTGAACTACTACTCAGAAGTACATCAAAACATTGGGCTACAGTTAGGATCCGAGGGCGCCAAAGACCATCTTTCAAAATCTCTGTTTCTCCTTGTGATTGGAAGCAATGACATCTTCGACTACGTTACAAAATCCAACCTCCAAAATGAATACACGCCACAGCAGTACACGGATTTAATGGCGTCGAATCTAAAGGAACAATTACAGAGAATACATGCTCTTGGTGCCCAAAAATTTGTAGTTGTCGGGACTGGGCCGGTTGGATGCTGTCCCTCGCAAAGGGATTCAAATACGGAAGAGTGCAATGATGGCGCAAACTCATTGTCTGTCATGTACAATGAAGCCCTTAAGTCCATGTTGCAGCAATTGAAACCGGAGTTGGGCATAAGCTACACCCTCTTCGACACTTACACTGTCTTGACCAACATCATACAAAACGCAGCTTCTTACGGATTTACAGAGGTTAAAGCTGCATGCTGTGGGATTGGGAAGCTTAACGCCATGGGTCCTTGCCTGCCAGTTGCATCCTTGTGCTCCAACAGAAGGGATCATGTCTTCTGGGATTTTTACCATCCTACACAGGCAACTCATGGTATCATAGTGGATAAACTTTTCGATGGCCCTTCAGAATACTCATCCCCAATGACTGTGAAGGAGTTAATTGCTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

369

Amino Acids

40.25

Weight (kDa)

5.75

Isoelectric Point (pI)

32.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 348 5.9e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 491, 659
AccI GTMKAC 1 cut(s) 348
AccIII TCCGGA 1 cut(s) 368
AclWI GGATC 3 cut(s) 477, 490, 990
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 4 cut(s) 162, 181, 247, 668
AcuI CTGAAG 1 cut(s) 1044
AcyI GRCGYC 2 cut(s) 492, 617
AfaI GTAC 3 cut(s) 456, 602, 770
AfiI CCNNNNNNNGG 4 cut(s) 194, 714, 815, 978
AflII CTTAAG 1 cut(s) 782
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 4 cut(s) 388, 510, 723, 805
AhlI ACTAGT 1 cut(s) 65
AjuI GAANNNNNNNTTGG 2 cut(s) 797, 829
AluBI AGCT 7 cut(s) 92, 360, 377, 825, 878, 905, 928
AluI AGCT 7 cut(s) 92, 360, 377, 825, 878, 905, 928
Alw21I GWGCWC 1 cut(s) 971
Alw26I GTCTC 1 cut(s) 117
AlwI GGATC 3 cut(s) 477, 490, 990
Aor13HI TCCGGA 1 cut(s) 368
AoxI GGCC 3 cut(s) 105, 689, 1054
ApeKI GCWGC 3 cut(s) 796, 875, 905
ApoI RAATTY 4 cut(s) 162, 181, 247, 668
ArsI GACNNNNNNTTYG 2 cut(s) 743, 775
Asp700I GAANNNNTTC 1 cut(s) 405
AspLEI GCGC 2 cut(s) 494, 749
AspS9I GGNCC 3 cut(s) 689, 941, 1055
AvaII GGWCC 1 cut(s) 941
BaeGI GKGCMC 1 cut(s) 664
BamHI GGATCC 1 cut(s) 482
BanI GGYRCC 2 cut(s) 491, 659
BbsI GAAGAC 2 cut(s) 40, 982
Bbv12I GWGCWC 1 cut(s) 971
BbvI GCAGC 3 cut(s) 808, 887, 892
BccI CCATC 5 cut(s) 91, 510, 737, 1014, 1046
BciVI GTATCC 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 117
BcuI ACTAGT 1 cut(s) 65
BfaI CTAG 2 cut(s) 66, 170
BfmI CTRYAG 2 cut(s) 252, 473
BfoI RGCGCY 1 cut(s) 495
BfrI CTTAAG 1 cut(s) 782
BfuI GTATCC 1 cut(s) 322
BisI GCNGC 3 cut(s) 797, 876, 906
BlsI GCNGC 3 cut(s) 798, 877, 907
Bme18I GGWCC 1 cut(s) 941
BmgT120I GGNCC 3 cut(s) 689, 941, 1055
BmiI GGNNCC 5 cut(s) 102, 484, 493, 661, 942
BmrI ACTGGG 1 cut(s) 696
BmsI GCATC 2 cut(s) 689, 968
BmuI ACTGGG 1 cut(s) 696
BpiI GAAGAC 2 cut(s) 40, 982
BsaAI YACGTR 1 cut(s) 115
BsaHI GRCGYC 2 cut(s) 492, 617
BsaJI CCNNGG 2 cut(s) 486, 936
BsaWI WCCGGW 2 cut(s) 368, 808
Bsc4I CCNNNNNNNGG 4 cut(s) 194, 714, 815, 978
Bse118I RCCGGY 1 cut(s) 691
Bse1I ACTGG 2 cut(s) 691, 953
Bse3DI GCAATG 3 cut(s) 244, 547, 745
BseAI TCCGGA 1 cut(s) 368
BseDI CCNNGG 2 cut(s) 486, 936
BseGI GGATG 5 cut(s) 403, 704, 959, 1006, 1070
BseLI CCNNNNNNNGG 4 cut(s) 194, 714, 815, 978
BseMI GCAATG 3 cut(s) 244, 547, 745
BseMII CTCAG 2 cut(s) 107, 462
BseNI ACTGG 2 cut(s) 691, 953
BseRI GAGGAG 1 cut(s) 387
BseSI GKGCMC 1 cut(s) 664
BseXI GCAGC 3 cut(s) 808, 887, 892
BshFI GGCC 3 cut(s) 107, 691, 1056
BshNI GGYRCC 2 cut(s) 491, 659
BsiHKAI GWGCWC 1 cut(s) 971
BsiSI CCGG 4 cut(s) 104, 369, 692, 809
BslFI GGGAC 2 cut(s) 690, 697
BslI CCNNNNNNNGG 4 cut(s) 194, 714, 815, 978
BsmAI GTCTC 1 cut(s) 117
BsmFI GGGAC 2 cut(s) 690, 697
BsnI GGCC 3 cut(s) 107, 691, 1056
Bsp1286I GDGCHC 2 cut(s) 664, 971
Bsp13I TCCGGA 1 cut(s) 368
Bsp1407I TGTACA 1 cut(s) 768
Bsp143I GATC 2 cut(s) 482, 982
Bsp19I CCATGG 1 cut(s) 936
BspANI GGCC 3 cut(s) 107, 691, 1056
BspCNI CTCAG 2 cut(s) 106, 461
BspEI TCCGGA 1 cut(s) 368
BspLI GGNNCC 5 cut(s) 102, 484, 493, 661, 942
BspMAI CTGCAG 1 cut(s) 256
BspPI GGATC 3 cut(s) 477, 490, 990
BspT107I GGYRCC 2 cut(s) 491, 659
BspTI CTTAAG 1 cut(s) 782
BsrDI GCAATG 3 cut(s) 244, 547, 745
BsrFI RCCGGY 1 cut(s) 691
BsrGI TGTACA 1 cut(s) 768
BsrI ACTGG 2 cut(s) 691, 953
BssAI RCCGGY 1 cut(s) 691
BssECI CCNNGG 2 cut(s) 486, 936
BssMI GATC 2 cut(s) 482, 982
BssNI GRCGYC 2 cut(s) 492, 617
BssT1I CCWWGG 1 cut(s) 936
Bst4CI ACNGT 4 cut(s) 288, 477, 850, 1084
Bst6I CTCTTC 3 cut(s) 62, 726, 839
BstACI GRCGYC 2 cut(s) 492, 617
BstAFI CTTAAG 1 cut(s) 782
BstAUI TGTACA 1 cut(s) 768
BstBAI YACGTR 1 cut(s) 115
BstC8I GCNNGC 2 cut(s) 910, 950
BstDEI CTNAG 2 cut(s) 93, 448
BstDSI CCRYGG 1 cut(s) 936
BstENI CCTNNNNNAGG 1 cut(s) 712
BstF5I GGATG 5 cut(s) 403, 704, 959, 1006, 1070
BstH2I RGCGCY 1 cut(s) 495
BstHHI GCGC 2 cut(s) 494, 749
BstKTI GATC 2 cut(s) 485, 985
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 2 cut(s) 482, 982
BstMWI GCNNNNNNNGC 2 cut(s) 659, 744
BstNSI RCATGY 2 cut(s) 653, 912
BstSFI CTRYAG 2 cut(s) 252, 473
BstSLI GKGCMC 1 cut(s) 664
BstV1I GCAGC 3 cut(s) 808, 887, 892
BstV2I GAAGAC 2 cut(s) 40, 982
BstX2I RGATCY 1 cut(s) 482
BstYI RGATCY 1 cut(s) 482
BsuI GTATCC 1 cut(s) 322
BsuRI GGCC 3 cut(s) 107, 691, 1056
BtgI CCRYGG 1 cut(s) 936
BtsCI GGATG 5 cut(s) 403, 704, 959, 1006, 1070
BtsIMutI CAGTG 2 cut(s) 15, 846
Cac8I GCNNGC 2 cut(s) 910, 950
CfoI GCGC 2 cut(s) 494, 749
Cfr10I RCCGGY 1 cut(s) 691
Cfr13I GGNCC 3 cut(s) 689, 941, 1055
CseI GACGC 1 cut(s) 606
Csp6I GTAC 3 cut(s) 455, 601, 769
CviAII CATG 7 cut(s) 650, 766, 790, 909, 937, 986, 1025
CviQI GTAC 3 cut(s) 455, 601, 769
DdeI CTNAG 2 cut(s) 93, 448
DinI GGCGCC 1 cut(s) 493
DpnI GATC 2 cut(s) 484, 984
DpnII GATC 2 cut(s) 482, 982
EaeI YGGCCR 1 cut(s) 105
Eam1104I CTCTTC 3 cut(s) 62, 726, 839
EarI CTCTTC 3 cut(s) 62, 726, 839
Eco130I CCWWGG 1 cut(s) 936
Eco47I GGWCC 1 cut(s) 941
Eco57I CTGAAG 1 cut(s) 1044
EcoNI CCTNNNNNAGG 1 cut(s) 712
EcoO109I RGGNCCY 1 cut(s) 941
EcoRI GAATTC 1 cut(s) 247
EcoT14I CCWWGG 1 cut(s) 936
EgeI GGCGCC 1 cut(s) 493
EheI GGCGCC 1 cut(s) 493
ErhI CCWWGG 1 cut(s) 936
FaeI CATG 7 cut(s) 653, 769, 793, 912, 940, 989, 1028
FalI AAGNNNNNCTT 2 cut(s) 489, 521
FaqI GGGAC 2 cut(s) 690, 697
FatI CATG 7 cut(s) 649, 765, 789, 908, 936, 985, 1024
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 3 cut(s) 797, 876, 906
FokI GGATG 5 cut(s) 410, 711, 946, 993, 1057
Fsp4HI GCNGC 3 cut(s) 797, 876, 906
FspBI CTAG 2 cut(s) 66, 170
GlaI GCGC 2 cut(s) 493, 748
GluI GCNGC 3 cut(s) 797, 876, 906
HaeII RGCGCY 1 cut(s) 495
HaeIII GGCC 3 cut(s) 107, 691, 1056
HapII CCGG 4 cut(s) 104, 369, 692, 809
HgaI GACGC 1 cut(s) 606
HhaI GCGC 2 cut(s) 494, 749
Hin1I GRCGYC 2 cut(s) 492, 617
Hin1II CATG 7 cut(s) 653, 769, 793, 912, 940, 989, 1028
Hin6I GCGC 2 cut(s) 492, 747
HinP1I GCGC 2 cut(s) 492, 747
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 926
HinfI GANTC 4 cut(s) 125, 381, 622, 719
HpaII CCGG 4 cut(s) 104, 369, 692, 809
Hpy166II GTNNAC 3 cut(s) 349, 439, 603
Hpy188I TCNGA 7 cut(s) 88, 96, 122, 397, 451, 487, 1063
Hpy188III TCNNGA 4 cut(s) 209, 369, 682, 853
Hpy8I GTNNAC 3 cut(s) 349, 439, 603
Hpy99I CGWCG 4 cut(s) 292, 350, 353, 622
HpyAV CCTTC 5 cut(s) 49, 352, 972, 1068, 1081
HpyCH4III ACNGT 4 cut(s) 288, 477, 850, 1084
HpyCH4IV ACGT 2 cut(s) 114, 558
HpyCH4V TGCA 5 cut(s) 254, 738, 796, 908, 959
HpyF10VI GCNNNNNNNGC 2 cut(s) 659, 744
HpyF3I CTNAG 2 cut(s) 93, 448
HpySE526I ACGT 2 cut(s) 114, 558
Hsp92I GRCGYC 2 cut(s) 492, 617
Hsp92II CATG 7 cut(s) 653, 769, 793, 912, 940, 989, 1028
HspAI GCGC 2 cut(s) 492, 747
KasI GGCGCC 1 cut(s) 491
Kpn2I TCCGGA 1 cut(s) 368
Kzo9I GATC 2 cut(s) 482, 982
LmnI GCTCC 2 cut(s) 374, 974
Lsp1109I GCAGC 3 cut(s) 808, 887, 892
LweI GCATC 2 cut(s) 689, 968
MaeI CTAG 2 cut(s) 66, 170
MaeII ACGT 2 cut(s) 114, 558
MaeIII GTNAC 4 cut(s) 76, 143, 279, 559
MalI GATC 2 cut(s) 484, 984
MboI GATC 2 cut(s) 482, 982
MboII GAAGA 7 cut(s) 40, 49, 376, 541, 743, 826, 982
MfeI CAATTG 1 cut(s) 800
MflI RGATCY 1 cut(s) 482
MhlI GDGCHC 2 cut(s) 664, 971
MluCI AATT 9 cut(s) 151, 162, 181, 247, 404, 635, 668, 800, 1095
Mly113I GGCGCC 1 cut(s) 492
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 3 cut(s) 594, 676, 996
MnlI CCTC 9 cut(s) 198, 222, 365, 376, 481, 584, 718, 842, 889
MroI TCCGGA 1 cut(s) 368
MroXI GAANNNNTTC 1 cut(s) 405
MseI TTAA 5 cut(s) 611, 783, 900, 930, 1094
MslI CAYNNNNRTG 3 cut(s) 18, 113, 964
MspCI CTTAAG 1 cut(s) 782
MspI CCGG 4 cut(s) 104, 369, 692, 809
MunI CAATTG 1 cut(s) 800
MwoI GCNNNNNNNGC 2 cut(s) 659, 744
NarI GGCGCC 1 cut(s) 492
NcoI CCATGG 1 cut(s) 936
NdeII GATC 2 cut(s) 482, 982
NlaIII CATG 7 cut(s) 653, 769, 793, 912, 940, 989, 1028
NlaIV GGNNCC 5 cut(s) 102, 484, 493, 661, 942
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 76
NspI RCATGY 2 cut(s) 653, 912
PaeI GCATGC 1 cut(s) 912
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 3 cut(s) 381, 622, 719
PkrI GCNGC 3 cut(s) 798, 877, 907
PleI GAGTC 1 cut(s) 119
PluTI GGCGCC 1 cut(s) 495
PpsI GAGTC 1 cut(s) 119
Ppu21I YACGTR 1 cut(s) 115
PpuMI RGGWCCY 1 cut(s) 941
Psp5II RGGWCCY 1 cut(s) 941
PspN4I GGNNCC 5 cut(s) 102, 484, 493, 661, 942
PspPI GGNCC 3 cut(s) 689, 941, 1055
PspPPI RGGWCCY 1 cut(s) 941
PstI CTGCAG 1 cut(s) 256
PsuI RGATCY 1 cut(s) 482
RsaI GTAC 3 cut(s) 456, 602, 770
RsaNI GTAC 3 cut(s) 455, 601, 769
RseI CAYNNNNRTG 3 cut(s) 18, 113, 964
SalI GTCGAC 1 cut(s) 347
SaqAI TTAA 5 cut(s) 611, 783, 900, 930, 1094
SatI GCNGC 3 cut(s) 797, 876, 906
Sau3AI GATC 2 cut(s) 482, 982
Sau96I GGNCC 3 cut(s) 689, 941, 1055
SchI GAGTC 1 cut(s) 119
SduI GDGCHC 2 cut(s) 664, 971
SfaNI GCATC 2 cut(s) 689, 968
SfcI CTRYAG 2 cut(s) 252, 473
SfoI GGCGCC 1 cut(s) 493
SgrDI CGTCGACG 1 cut(s) 347
SinI GGWCC 1 cut(s) 941
SmiMI CAYNNNNRTG 3 cut(s) 18, 113, 964
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
SpeI ACTAGT 1 cut(s) 65
SphI GCATGC 1 cut(s) 912
Sse9I AATT 9 cut(s) 151, 162, 181, 247, 404, 635, 668, 800, 1095
SspDI GGCGCC 1 cut(s) 491
SspMI CTAG 2 cut(s) 66, 170
StyI CCWWGG 1 cut(s) 936
TaaI ACNGT 4 cut(s) 288, 477, 850, 1084
TaiI ACGT 2 cut(s) 117, 561
TaqI TCGA 5 cut(s) 348, 552, 620, 837, 1050
TasI AATT 9 cut(s) 151, 162, 181, 247, 404, 635, 668, 800, 1095
TatI WGTACW 3 cut(s) 454, 600, 768
TfiI GAWTC 3 cut(s) 381, 622, 719
Tru1I TTAA 5 cut(s) 611, 783, 900, 930, 1094
Tru9I TTAA 5 cut(s) 611, 783, 900, 930, 1094
TscAI CASTG 2 cut(s) 22, 853
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 3 cut(s) 796, 875, 905
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 5 cut(s) 49, 61, 414, 597, 789
TspGWI ACGGA 4 cut(s) 366, 620, 743, 900
TspRI CASTG 2 cut(s) 22, 853
Vha464I CTTAAG 1 cut(s) 782
VpaK11BI GGWCC 1 cut(s) 941
XagI CCTNNNNNAGG 1 cut(s) 712
XapI RAATTY 4 cut(s) 162, 181, 247, 668
XceI RCATGY 2 cut(s) 653, 912
XmiI GTMKAC 1 cut(s) 348
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 2 cut(s) 66, 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.