Rh5BG229900

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002678
Physical Location & Seq
Forward (+)
27005199 .. 27006857
1659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG229900.1

Sequence Viewer

Length: 672 bp
ATGACCATGGAAGCTTGCAGTGTTTCTCCTACGATTTTCTTCACCATCTTCTCCATTATCGTTTCTGTGTTCATCGCTTTCTCAAATGCTCAGACGGTTCCGGCCATTTACATATTTGGAGACTCTCTATTGGATGTTGGTAACAACAATTACCTTACCATCTCTGTGGCCAAGGCAAATTTTCCTCACAACGGTATCGATTTTCCGACCAAGAAACCAACCGGGAGGTTTTGCAATGGCAAGAATTCTGCAGATTTTCTGGCTGAGAAAGTAGGATTGCCTACTTCCCCACCATATCTGTCGCTTTTATCAAAATCTAATAAGCAAAAGGGGTCCTTCGAAGCTGGAGTAAGCTTCGCCTCTGGAGGGGCTGGAATCTTCAACGGCACCGATGATGAACATCGTCAGTCACTACCTTTGACAAAACAAATAGACTACTATTCACAAGTTTGTGAAGAACTTCGGCAACAATTAGGAGCTCAAGCTGCCCAAGACCATCTATCGAAATCTCTACATGTCGTTCTTATTGGAAGCAATGACGTTTTCGGCTACTTAGATTCATCCAGCACCCAAAAGCAATACACACCGGATCAGTATGTGGATTTGATGCTGTCTACTCTTAAACAACACTTAAAGTCGATCGGACAACATAACAAGCTCGATATTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

223

Amino Acids

24.31

Weight (kDa)

6.28

Isoelectric Point (pI)

29.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 214 7.1e-13 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 386
AccI GTMKAC 1 cut(s) 614
AclWI GGATC 1 cut(s) 597
AcoI YGGCCR 2 cut(s) 102, 168
AcsI RAATTY 2 cut(s) 178, 244
AfiI CCNNNNNNNGG 2 cut(s) 191, 366
AflIII ACRYGT 1 cut(s) 514
AgsI TTSAA 1 cut(s) 382
AluBI AGCT 6 cut(s) 14, 344, 354, 479, 485, 658
AluI AGCT 6 cut(s) 14, 344, 354, 479, 485, 658
Alw21I GWGCWC 1 cut(s) 481
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 1 cut(s) 597
AoxI GGCC 2 cut(s) 102, 168
ApeKI GCWGC 1 cut(s) 485
ApoI RAATTY 2 cut(s) 178, 244
Asp700I GAANNNNTTC 1 cut(s) 459
AspS9I GGNCC 1 cut(s) 333
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 1 cut(s) 34
AsuII TTCGAA 1 cut(s) 339
AvaII GGWCC 1 cut(s) 333
BaeI ACNNNNGTAYC 2 cut(s) 178, 211
BalI TGGCCA 1 cut(s) 170
BanI GGYRCC 1 cut(s) 386
BanII GRGCYC 1 cut(s) 481
Bbv12I GWGCWC 1 cut(s) 481
BbvI GCAGC 1 cut(s) 472
BccI CCATC 3 cut(s) 53, 167, 504
BceAI ACGGC 1 cut(s) 400
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 114
BfmI CTRYAG 1 cut(s) 249
BisI GCNGC 1 cut(s) 486
BlsI GCNGC 1 cut(s) 487
Bme1390I CCNGG 1 cut(s) 223
Bme18I GGWCC 1 cut(s) 333
BmgT120I GGNCC 1 cut(s) 333
BmiI GGNNCC 3 cut(s) 99, 334, 388
BmrFI CCNGG 1 cut(s) 223
BmsI GCATC 1 cut(s) 597
BpmI CTGGAG 2 cut(s) 366, 384
Bpu14I TTCGAA 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 465
BpuMI CCSGG 1 cut(s) 223
Bsa29I ATCGAT 1 cut(s) 198
BsaBI GATNNNNATC 1 cut(s) 399
BsaJI CCNNGG 2 cut(s) 6, 171
BsaWI WCCGGW 1 cut(s) 586
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 366
Bse3DI GCAATG 2 cut(s) 241, 541
Bse8I GATNNNNATC 1 cut(s) 399
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 2 cut(s) 6, 171
BseGI GGATG 2 cut(s) 139, 560
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 2 cut(s) 191, 366
BseMI GCAATG 2 cut(s) 241, 541
BseMII CTCAG 2 cut(s) 104, 255
BseXI GCAGC 1 cut(s) 472
Bsh1285I CGRYCG 1 cut(s) 642
BshFI GGCC 2 cut(s) 104, 170
BshNI GGYRCC 1 cut(s) 386
BshVI ATCGAT 1 cut(s) 198
BsiEI CGRYCG 1 cut(s) 642
BsiHKAI GWGCWC 1 cut(s) 481
BsiSI CCGG 3 cut(s) 101, 222, 587
BslI CCNNNNNNNGG 2 cut(s) 191, 366
BsmAI GTCTC 1 cut(s) 114
BsnI GGCC 2 cut(s) 104, 170
Bsp119I TTCGAA 1 cut(s) 339
Bsp1286I GDGCHC 1 cut(s) 481
Bsp143I GATC 2 cut(s) 589, 639
Bsp19I CCATGG 1 cut(s) 6
BspANI GGCC 2 cut(s) 104, 170
BspCNI CTCAG 2 cut(s) 103, 256
BspDI ATCGAT 1 cut(s) 198
BspLI GGNNCC 3 cut(s) 99, 334, 388
BspMAI CTGCAG 1 cut(s) 253
BspPI GGATC 1 cut(s) 597
BspT104I TTCGAA 1 cut(s) 339
BspT107I GGYRCC 1 cut(s) 386
BsrDI GCAATG 2 cut(s) 241, 541
BssECI CCNNGG 2 cut(s) 6, 171
BssMI GATC 2 cut(s) 589, 639
BssT1I CCWWGG 2 cut(s) 6, 171
Bst4CI ACNGT 2 cut(s) 97, 194
BstBI TTCGAA 1 cut(s) 339
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 3 cut(s) 90, 264, 553
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 2 cut(s) 139, 560
BstKTI GATC 2 cut(s) 592, 642
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 2 cut(s) 589, 639
BstMCI CGRYCG 1 cut(s) 642
BstMWI GCNNNNNNNGC 1 cut(s) 485
BstNSI RCATGY 1 cut(s) 518
BstSCI CCNGG 1 cut(s) 221
BstSFI CTRYAG 1 cut(s) 249
BstV1I GCAGC 1 cut(s) 472
BstXI CCANNNNNNTGG 1 cut(s) 166
Bsu15I ATCGAT 1 cut(s) 198
BsuRI GGCC 2 cut(s) 104, 170
BsuTUI ATCGAT 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 6
BtgZI GCGATG 1 cut(s) 58
BtsCI GGATG 2 cut(s) 139, 560
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 1 cut(s) 25
Cac8I GCNNGC 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 333
ClaI ATCGAT 1 cut(s) 198
CviAII CATG 2 cut(s) 7, 515
DdeI CTNAG 3 cut(s) 90, 264, 553
DpnI GATC 2 cut(s) 591, 641
DpnII GATC 2 cut(s) 589, 639
EaeI YGGCCR 2 cut(s) 102, 168
Ecl136II GAGCTC 1 cut(s) 479
Eco130I CCWWGG 2 cut(s) 6, 171
Eco24I GRGCYC 1 cut(s) 481
Eco47I GGWCC 1 cut(s) 333
Eco53kI GAGCTC 1 cut(s) 479
EcoICRI GAGCTC 1 cut(s) 479
EcoO109I RGGNCCY 1 cut(s) 333
EcoRI GAATTC 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 6, 171
EcoT38I GRGCYC 1 cut(s) 481
ErhI CCWWGG 2 cut(s) 6, 171
FaeI CATG 2 cut(s) 10, 518
FaiI YATR 6 cut(s) 8, 113, 295, 516, 597, 651
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FatI CATG 2 cut(s) 6, 514
FblI GTMKAC 1 cut(s) 614
Fnu4HI GCNGC 1 cut(s) 486
FokI GGATG 2 cut(s) 146, 547
FriOI GRGCYC 1 cut(s) 481
Fsp4HI GCNGC 1 cut(s) 486
GluI GCNGC 1 cut(s) 486
GsuI CTGGAG 2 cut(s) 366, 384
HaeIII GGCC 2 cut(s) 104, 170
HapII CCGG 3 cut(s) 101, 222, 587
Hin1II CATG 2 cut(s) 10, 518
HindIII AAGCTT 2 cut(s) 12, 352
HinfI GANTC 3 cut(s) 122, 375, 557
HpaII CCGG 3 cut(s) 101, 222, 587
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 1 cut(s) 615
Hpy188I TCNGA 3 cut(s) 93, 207, 644
Hpy188III TCNNGA 1 cut(s) 363
Hpy8I GTNNAC 1 cut(s) 615
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 2 cut(s) 97, 194
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 3 cut(s) 18, 234, 251
HpyF10VI GCNNNNNNNGC 1 cut(s) 485
HpyF3I CTNAG 3 cut(s) 90, 264, 553
HpySE526I ACGT 1 cut(s) 540
Hsp92II CATG 2 cut(s) 10, 518
Kzo9I GATC 2 cut(s) 589, 639
LmnI GCTCC 1 cut(s) 476
LpnPI CCDG 8 cut(s) 114, 235, 245, 330, 348, 357, 577, 600
Lsp1109I GCAGC 1 cut(s) 472
LweI GCATC 1 cut(s) 597
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 2 cut(s) 140, 408
MalI GATC 2 cut(s) 591, 641
MboI GATC 2 cut(s) 589, 639
MboII GAAGA 4 cut(s) 31, 40, 370, 467
MhlI GDGCHC 1 cut(s) 481
MlsI TGGCCA 1 cut(s) 170
MluCI AATT 4 cut(s) 148, 178, 244, 470
MluNI TGGCCA 1 cut(s) 170
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 230
MnlI CCTC 4 cut(s) 195, 219, 359, 370
Mox20I TGGCCA 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 459
MscI TGGCCA 1 cut(s) 170
MseI TTAA 2 cut(s) 621, 632
MslI CAYNNNNRTG 1 cut(s) 164
Msp20I TGGCCA 1 cut(s) 170
MspI CCGG 3 cut(s) 101, 222, 587
MspR9I CCNGG 1 cut(s) 223
MwoI GCNNNNNNNGC 1 cut(s) 485
NciI CCSGG 1 cut(s) 223
NcoI CCATGG 1 cut(s) 6
NdeII GATC 2 cut(s) 589, 639
NlaIII CATG 2 cut(s) 10, 518
NlaIV GGNNCC 3 cut(s) 99, 334, 388
NmuCI GTSAC 1 cut(s) 408
NspI RCATGY 1 cut(s) 518
NspV TTCGAA 1 cut(s) 339
PciI ACATGT 1 cut(s) 514
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 2 cut(s) 375, 557
PkrI GCNGC 1 cut(s) 487
Ple19I CGATCG 1 cut(s) 642
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
PpuMI RGGWCCY 1 cut(s) 333
PscI ACATGT 1 cut(s) 514
Psp124BI GAGCTC 1 cut(s) 481
Psp5II RGGWCCY 1 cut(s) 333
PspN4I GGNNCC 3 cut(s) 99, 334, 388
PspPI GGNCC 1 cut(s) 333
PspPPI RGGWCCY 1 cut(s) 333
PstI CTGCAG 1 cut(s) 253
PvuI CGATCG 1 cut(s) 642
RseI CAYNNNNRTG 1 cut(s) 164
SacI GAGCTC 1 cut(s) 481
SaqAI TTAA 2 cut(s) 621, 632
SatI GCNGC 1 cut(s) 486
Sau3AI GATC 2 cut(s) 589, 639
Sau96I GGNCC 1 cut(s) 333
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 1 cut(s) 223
SduI GDGCHC 1 cut(s) 481
SfaNI GCATC 1 cut(s) 597
SfcI CTRYAG 1 cut(s) 249
SfuI TTCGAA 1 cut(s) 339
SinI GGWCC 1 cut(s) 333
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 480
SmoI CTYRAG 1 cut(s) 480
Sse9I AATT 4 cut(s) 148, 178, 244, 470
SstI GAGCTC 1 cut(s) 481
StyD4I CCNGG 1 cut(s) 221
StyI CCWWGG 2 cut(s) 6, 171
TaaI ACNGT 2 cut(s) 97, 194
TaiI ACGT 1 cut(s) 543
TaqI TCGA 5 cut(s) 198, 339, 503, 638, 660
TasI AATT 4 cut(s) 148, 178, 244, 470
TfiI GAWTC 2 cut(s) 375, 557
Tru1I TTAA 2 cut(s) 621, 632
Tru9I TTAA 2 cut(s) 621, 632
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 1 cut(s) 408
TseI GCWGC 1 cut(s) 485
Tsp45I GTSAC 1 cut(s) 408
TspDTI ATGAA 3 cut(s) 61, 411, 549
TspRI CASTG 1 cut(s) 25
VpaK11BI GGWCC 1 cut(s) 333
XapI RAATTY 2 cut(s) 178, 244
XceI RCATGY 1 cut(s) 518
XmiI GTMKAC 1 cut(s) 614
XmnI GAANNNNTTC 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.