Rorug05G0136800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002678
Physical Location & Seq
Forward (+)
12351059 .. 12353722
2664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0136800.1

Sequence Viewer

Length: 2664 bp
ATGGCCTCAGTAGTAACGGACCTCTTAATTGGAAAACTTGCGACAATTCTTGAGAACGAAGGAACAGCCATAGCTGGTATTCGTGATGAAGTTGATAAGATCAAGGAGGAGTTTCTAAGCATGAAAGCTTTCCTAGTGGATGCAGAGGCCAACAAAGCAAAAACTGAAGGAGAGAAATTGTGGGTTGCAAGAATCAGAAACCTAGCCTATGATGTTGAAGACATCATTGATGAATTCATGTATCACATGTATGAGAAGCAAAGTGGGGGTCGACTTTCAAGGGGGCTACACAAAACCATTCGCGCTCCATGTAATCTTTGGTTCAGGCATAAAATTGCAAAGAAGTTGCAGAAAATTACTGAAATGATCAGAGCCATTCCAGAGAGGAATCAAAGATACAGTGTTGGCCTTGTTGGAGGAGCAACTACATCTGAAGATATTCACAAATTGTTGCAGAGCCAAGCGGAGTCTTCGTTTTTCATTAGGGAAGACGAACTCGTTGGGATTAAAGGCAAGAAGCAAATACTAATAGGATGGCTTATGGATGAAGAGCAACACCAAACTGTTATATCTGTGGTTGGCATGGGAGGTTCTGGGAAGACAACTCTAGTTGCCAAGGTCTTCAACAGTGAAAGGGTGAAGAGACATTTCGATTGTTATGCATGGATTACAGTTTCCCAAACTTATGATGTGGTAGACTTGTTTCGAAAGTTGATCAGGGAACTCTACAAATCAAGGAAGGAAGAGTACCCTGCAAATTTGAATGCCATGAACCGCAAAGATTTGCTAGAGTTACTAATTAACTACTTGGACTCCAAAAGATACCTGGTTGTTCTAGATGACGTGTGGGATATAAAAATTTGGAGCGAAATAAATGTATCACTTCAAGATAGGCAACTCGGAAGTCGAATCATAGTTACAACTCGAAAAGAAGATGTAGCATCCCATTCTTTTGGAGTTAAAAGCCATGTTCACCATATTCAACCCCTTCTAAAGAATGAGGCTTGGGAGGTCTTCTGCAAAAAAGCATTTTCATCTAATGAGTACAAAACTTGTCCACCTGATTTTGTGTCGTTAGCTTGCCAACTTGTGGAGAAGTGTGAAGGCCTTCCTCTGGCTATTGTGGCTTTAGGTGGTCTTATGTCTTCCAAGAAGTCGCTGGATCAATGGGAGCAAGTCTGCAACAGCTTGAATTGGCATCTAGATCATAGTCCATTGCTAGACCCTGTGAAAAATATCTTGCTTCTGAGTTTCAATGATTTGCCATCTCAATTGAGACATTGCTTCCTCTATTTCTCTCTATTCCCAGAAGATTTTGTAATTAAAAGAAAAAGACTCGTTAGATTGTGGATGGCCGAAGGATTTGTTGAACATGTGAAAGGGATCACGCCAGAAGAAGTTGCAGATGACTATCTTTGGGAACTCTGTTTCCGCAGTATGTTACAAGTTGTGAGGAGAAATAATACAGGAAGGCCGAAACAAGTTAAGATGCATGATCTGATGCGAGAGCTTGCTTTGTCGACAGCCGAGAAGGAAAAGTTTGGTCATGTATACGACGGGAAAGAAGTAATGGAAGGCATCTCAATCCGTCGCTTGTCAATTCACAGAATGGAAGGAGAAATTAAATCATGCCCGGGTATGTCAAACATTCGTTCTCTTCTAGTATTTGCCCCTGACATGTCCTCATTGTGTTTCTCAAATACACTGGTGTCTCGGTTCAAATTATTGAGGACTCTAGAGTTGCAGGAAGTCCAAATTGATAAACTGCCGGGTGAAGTTGTTTACTTGTTCAACTTGAGATATTTAAATTTGAAGGGCACTTTAATCAAGCAACTTCCAGAGTCTATAGGGCGTCTCCGCAACCTTCAATTACTGAACATCAAGAATACTAAGATAGAATCACTTCCAAAAGAAATTGCAAAGTTGCTGAACCTGCGCCATCTAGTTATGTATCGCTTCACTGGAAACTATCTGGACTTCAAATATGTGAGTGGGATGAAAGTGTCATCGAATATTTCTCAGTTGCAGAAATTGCAAACTTTATCGATGATTGAATCAGAAGGGGACACTATTAGACATATCGGCAACCTGACTCAACTTAAGAGCATCGGCATATCGAATGTGAAAGAAAGAGATGAGATTGACCTTTGGGTCTCAATTGAAAAGCTGAATCTGCTTCAATCTGTGGTTTTAATGGCAAGTAATGAAGAGGAAATCCTTCCAGTGAAAGCACAATGTCCACGTCTTCCGCGCCTTCGAATACTTTATTTGATTGGCAGACTGCAAAACGCAATACCTTGGTTTTCTTCACTGCAGAGCCTCGGACGTCTGTGTCTGCGTTGGTCTAGACTTGAAGATGATTTACTACCTCAAATTGAAGCACTGCCCTCTCTGACATGGCTTGATCTTTGGAATTCATATGTTGGGGAAGAGTTGTGTTTCCGCAGAGGCTTTGTAAAGTTGGAGAGGTTGGAGTTGTTCAATTTTGCCTCGGTGAAAAAGATTAGTATAGAAAAAGGGGTGATGCCAAATCTCCGGTACTTATGGGTTAAAAACTGCATGGAGTTGAAAACAGTGCCATTGGGCCTTGAATACCTTTCTAATCTACATTCTTTGGCTCTGGAATTTGTTCCTGATACGCTTAAAAGCAAGCGCATAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

887

Amino Acids

102.28

Weight (kDa)

8.89

Isoelectric Point (pI)

51.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 90 1.6e-22 Rx N-terminal domain
NB-ARC PF00931 173 - 347 6e-48 NB-ARC domain
AAA_22 PF13401 185 - 283 2.6e-06 AAA domain
AAA_16 PF13191 188 - 282 2e-06 AAA ATPase domain
WHD_DRP PF23559 434 - 505 2e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 550 - 876 4.8e-40 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 2150, 2331
AatII GACGTC 1 cut(s) 2329
Acc36I ACCTGC 1 cut(s) 1941
AccB7I CCANNNNNTGG 1 cut(s) 1090
AccI GTMKAC 4 cut(s) 271, 696, 1520, 1551
AccII CGCG 2 cut(s) 303, 2251
AciI CCGC 6 cut(s) 464, 775, 1432, 1858, 2249, 2443
AclWI GGATC 2 cut(s) 1170, 1391
AcoI YGGCCR 1 cut(s) 1353
AcsI RAATTY 6 cut(s) 233, 757, 858, 1807, 2413, 2624
AcuI CTGAAG 2 cut(s) 186, 453
AcyI GRCGYC 2 cut(s) 1852, 2326
AfaI GTAC 3 cut(s) 749, 1046, 2540
AfiI CCNNNNNNNGG 2 cut(s) 1090, 1114
AflII CTTAAG 1 cut(s) 2099
AflIII ACRYGT 4 cut(s) 246, 843, 1372, 1677
AjiI CACGTC 2 cut(s) 844, 2243
AjnI CCWGG 1 cut(s) 825
AloI GAACNNNNNNTCC 2 cut(s) 1413, 1445
AluBI AGCT 6 cut(s) 74, 128, 1079, 1188, 1510, 2167
AluI AGCT 6 cut(s) 74, 128, 1079, 1188, 1510, 2167
Alw26I GTCTC 5 cut(s) 637, 1270, 1716, 1859, 2158
AlwI GGATC 2 cut(s) 1170, 1391
Ama87I CYCGRG 1 cut(s) 1633
AoxI GGCC 7 cut(s) 3, 147, 406, 1105, 1353, 1472, 2584
ApoI RAATTY 6 cut(s) 233, 757, 858, 1807, 2413, 2624
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 6 cut(s) 128, 438, 1107, 1902, 2217, 2628
AspLEI GCGC 4 cut(s) 305, 1938, 2253, 2655
AspS9I GGNCC 2 cut(s) 19, 2584
AsuC2I CCSGG 3 cut(s) 1634, 1635, 1770
AsuHPI GGTGA 5 cut(s) 649, 965, 1784, 2506, 2533
AsuII TTCGAA 2 cut(s) 706, 2257
AvaI CYCGRG 1 cut(s) 1633
AvaII GGWCC 1 cut(s) 19
BaeGI GKGCMC 1 cut(s) 1820
BarI GAAGNNNNNNTAC 4 cut(s) 731, 763, 2346, 2378
BbsI GAAGAC 8 cut(s) 225, 462, 495, 605, 613, 1006, 1137, 2237
BccI CCATC 4 cut(s) 528, 1273, 1345, 1947
BcgI CGANNNNNNTGC 2 cut(s) 641, 675
BciT130I CCWGG 1 cut(s) 827
BclI TGATCA 2 cut(s) 366, 714
BcnI CCSGG 3 cut(s) 1634, 1635, 1770
BcoDI GTCTC 5 cut(s) 637, 1270, 1716, 1859, 2158
BfmI CTRYAG 2 cut(s) 1845, 2312
BfrI CTTAAG 1 cut(s) 2099
BfuAI ACCTGC 1 cut(s) 1941
Bme1390I CCNGG 4 cut(s) 827, 1634, 1635, 1770
Bme18I GGWCC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 1633
BmgBI CACGTC 2 cut(s) 844, 2243
BmgT120I GGNCC 2 cut(s) 19, 2584
BmrFI CCNGG 4 cut(s) 827, 1634, 1635, 1770
BmsI GCATC 8 cut(s) 130, 950, 1207, 1479, 1491, 1587, 2115, 2514
BpiI GAAGAC 8 cut(s) 225, 462, 495, 605, 613, 1006, 1137, 2237
Bpu14I TTCGAA 2 cut(s) 706, 2257
BpuEI CTTGAG 2 cut(s) 71, 1816
BpuMI CCSGG 3 cut(s) 1634, 1635, 1770
Bsa29I ATCGAT 1 cut(s) 2045
BsaBI GATNNNNATC 1 cut(s) 1410
BsaHI GRCGYC 2 cut(s) 1852, 2326
BsaI GGTCTC 1 cut(s) 2158
BsaJI CCNNGG 5 cut(s) 615, 1633, 2297, 2320, 2490
BsaWI WCCGGW 1 cut(s) 2535
BsaXI ACNNNNNCTCC 4 cut(s) 458, 488, 797, 827
Bsc4I CCNNNNNNNGG 2 cut(s) 1090, 1114
Bse1I ACTGG 3 cut(s) 1710, 1966, 2222
Bse3DI GCAATG 2 cut(s) 1214, 1279
Bse8I GATNNNNATC 1 cut(s) 1410
BseBI CCWGG 1 cut(s) 827
BseCI ATCGAT 1 cut(s) 2045
BseDI CCNNGG 5 cut(s) 615, 1633, 2297, 2320, 2490
BseGI GGATG 6 cut(s) 145, 539, 550, 941, 1356, 2001
BseJI GATNNNNATC 1 cut(s) 1410
BseLI CCNNNNNNNGG 2 cut(s) 1090, 1114
BseMI GCAATG 2 cut(s) 1214, 1279
BseMII CTCAG 3 cut(s) 21, 1238, 2033
BseNI ACTGG 3 cut(s) 1710, 1966, 2222
BseRI GAGGAG 3 cut(s) 122, 432, 1468
BseSI GKGCMC 1 cut(s) 1820
Bsh1236I CGCG 2 cut(s) 303, 2251
BshFI GGCC 7 cut(s) 5, 149, 408, 1107, 1355, 1474, 2586
BshVI ATCGAT 1 cut(s) 2045
BsiHKCI CYCGRG 1 cut(s) 1633
BsiSI CCGG 3 cut(s) 1634, 1769, 2536
BslFI GGGAC 1 cut(s) 2078
BslI CCNNNNNNNGG 2 cut(s) 1090, 1114
BsmAI GTCTC 5 cut(s) 637, 1270, 1716, 1859, 2158
BsmBI CGTCTC 1 cut(s) 1859
BsmFI GGGAC 1 cut(s) 2078
BsmI GAATGC 1 cut(s) 769
BsnI GGCC 7 cut(s) 5, 149, 408, 1107, 1355, 1474, 2586
Bso31I GGTCTC 1 cut(s) 2158
BsoBI CYCGRG 1 cut(s) 1633
Bsp119I TTCGAA 2 cut(s) 706, 2257
Bsp1286I GDGCHC 1 cut(s) 1820
Bsp143I GATC 8 cut(s) 99, 366, 714, 1162, 1204, 1383, 1495, 2404
BspACI CCGC 6 cut(s) 464, 775, 1432, 1858, 2249, 2443
BspANI GGCC 7 cut(s) 5, 149, 408, 1107, 1355, 1474, 2586
BspCNI CTCAG 3 cut(s) 20, 1239, 2032
BspDI ATCGAT 1 cut(s) 2045
BspFNI CGCG 2 cut(s) 303, 2251
BspMAI CTGCAG 1 cut(s) 2316
BspMI ACCTGC 1 cut(s) 1941
BspPI GGATC 2 cut(s) 1170, 1391
BspQI GCTCTTC 1 cut(s) 543
BspT104I TTCGAA 2 cut(s) 706, 2257
BspTI CTTAAG 1 cut(s) 2099
BspTNI GGTCTC 1 cut(s) 2158
BsrDI GCAATG 2 cut(s) 1214, 1279
BsrI ACTGG 3 cut(s) 1710, 1966, 2222
BssECI CCNNGG 5 cut(s) 615, 1633, 2297, 2320, 2490
BssMI GATC 8 cut(s) 99, 366, 714, 1162, 1204, 1383, 1495, 2404
BssNAI GTATAC 1 cut(s) 1552
BssNI GRCGYC 2 cut(s) 1852, 2326
BssT1I CCWWGG 2 cut(s) 615, 2297
Bst1107I GTATAC 1 cut(s) 1552
Bst2UI CCWGG 1 cut(s) 827
Bst4CI ACNGT 5 cut(s) 401, 565, 629, 673, 2575
Bst6I CTCTTC 6 cut(s) 543, 635, 738, 1662, 2202, 2424
BstACI GRCGYC 2 cut(s) 1852, 2326
BstAFI CTTAAG 1 cut(s) 2099
BstAPI GCANNNNNTGC 1 cut(s) 2032
BstBI TTCGAA 2 cut(s) 706, 2257
BstC8I GCNNGC 3 cut(s) 1081, 1512, 2651
BstDEI CTNAG 5 cut(s) 7, 116, 1247, 1890, 2019
BstF5I GGATG 6 cut(s) 145, 539, 550, 941, 1356, 2001
BstFNI CGCG 2 cut(s) 303, 2251
BstHHI GCGC 4 cut(s) 305, 1938, 2253, 2655
BstKTI GATC 8 cut(s) 102, 369, 717, 1165, 1207, 1386, 1498, 2407
BstMAI GTCTC 5 cut(s) 637, 1270, 1716, 1859, 2158
BstMBI GATC 8 cut(s) 99, 366, 714, 1162, 1204, 1383, 1495, 2404
BstMWI GCNNNNNNNGC 5 cut(s) 155, 1124, 1933, 2032, 2173
BstNI CCWGG 1 cut(s) 827
BstNSI RCATGY 3 cut(s) 250, 1376, 1681
BstSCI CCNGG 4 cut(s) 825, 1632, 1633, 1768
BstSFI CTRYAG 2 cut(s) 1845, 2312
BstSLI GKGCMC 1 cut(s) 1820
BstUI CGCG 2 cut(s) 303, 2251
BstV2I GAAGAC 8 cut(s) 225, 462, 495, 605, 613, 1006, 1137, 2237
BstXI CCANNNNNNTGG 1 cut(s) 953
BstZ17I GTATAC 1 cut(s) 1552
Bsu15I ATCGAT 1 cut(s) 2045
BsuRI GGCC 7 cut(s) 5, 149, 408, 1107, 1355, 1474, 2586
BsuTUI ATCGAT 1 cut(s) 2045
BtrI CACGTC 2 cut(s) 844, 2243
BtsCI GGATG 6 cut(s) 145, 539, 550, 941, 1356, 2001
BtsI GCAGTG 2 cut(s) 2309, 2381
BtsIMutI CAGTG 8 cut(s) 406, 634, 1703, 1959, 2229, 2309, 2381, 2580
BveI ACCTGC 1 cut(s) 1941
Cac8I GCNNGC 3 cut(s) 1081, 1512, 2651
CfoI GCGC 4 cut(s) 305, 1938, 2253, 2655
Cfr13I GGNCC 2 cut(s) 19, 2584
Cfr9I CCCGGG 1 cut(s) 1633
ClaI ATCGAT 1 cut(s) 2045
CseI GACGC 1 cut(s) 1841
CsiI ACCWGGT 1 cut(s) 825
Csp6I GTAC 3 cut(s) 748, 1045, 2539
CviQI GTAC 3 cut(s) 748, 1045, 2539
DdeI CTNAG 5 cut(s) 7, 116, 1247, 1890, 2019
DpnI GATC 8 cut(s) 101, 368, 716, 1164, 1206, 1385, 1497, 2406
DpnII GATC 8 cut(s) 99, 366, 714, 1162, 1204, 1383, 1495, 2404
DraI TTTAAA 1 cut(s) 1806
DrdI GACNNNNNNGTC 2 cut(s) 2150, 2331
DseDI GACNNNNNNGTC 2 cut(s) 2150, 2331
EaeI YGGCCR 1 cut(s) 1353
Eam1104I CTCTTC 6 cut(s) 543, 635, 738, 1662, 2202, 2424
EarI CTCTTC 6 cut(s) 543, 635, 738, 1662, 2202, 2424
Eco130I CCWWGG 2 cut(s) 615, 2297
Eco147I AGGCCT 1 cut(s) 1107
Eco31I GGTCTC 1 cut(s) 2158
Eco47I GGWCC 1 cut(s) 19
Eco57I CTGAAG 2 cut(s) 186, 453
Eco88I CYCGRG 1 cut(s) 1633
EcoRI GAATTC 2 cut(s) 233, 2413
EcoRII CCWGG 1 cut(s) 825
EcoT14I CCWWGG 2 cut(s) 615, 2297
EcoT22I ATGCAT 2 cut(s) 664, 1494
ErhI CCWWGG 2 cut(s) 615, 2297
Esp3I CGTCTC 1 cut(s) 1859
FaqI GGGAC 1 cut(s) 2078
FauNDI CATATG 1 cut(s) 2419
FbaI TGATCA 2 cut(s) 366, 714
FblI GTMKAC 4 cut(s) 271, 696, 1520, 1551
FokI GGATG 6 cut(s) 152, 546, 557, 928, 1363, 2008
GlaI GCGC 4 cut(s) 304, 1937, 2252, 2654
HaeIII GGCC 7 cut(s) 5, 149, 408, 1107, 1355, 1474, 2586
HapII CCGG 3 cut(s) 1634, 1769, 2536
HgaI GACGC 1 cut(s) 1841
HhaI GCGC 4 cut(s) 305, 1938, 2253, 2655
Hin1I GRCGYC 2 cut(s) 1852, 2326
Hin6I GCGC 4 cut(s) 303, 1936, 2251, 2653
HinP1I GCGC 4 cut(s) 303, 1936, 2251, 2653
HincII GTYRAC 2 cut(s) 272, 1521
HindII GTYRAC 2 cut(s) 272, 1521
HindIII AAGCTT 1 cut(s) 126
HpaII CCGG 3 cut(s) 1634, 1769, 2536
HphI GGTGA 5 cut(s) 649, 965, 1784, 2506, 2533
Hpy166II GTNNAC 8 cut(s) 272, 697, 973, 1058, 1521, 1552, 1783, 2240
Hpy188I TCNGA 9 cut(s) 197, 371, 433, 902, 1248, 1500, 2059, 2324, 2394
Hpy8I GTNNAC 8 cut(s) 272, 697, 973, 1058, 1521, 1552, 1783, 2240
Hpy99I CGWCG 2 cut(s) 1559, 1593
HpyCH4III ACNGT 5 cut(s) 401, 565, 629, 673, 2575
HpyCH4IV ACGT 3 cut(s) 843, 2242, 2326
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 1124, 1933, 2032, 2173
HpyF3I CTNAG 5 cut(s) 7, 116, 1247, 1890, 2019
HpySE526I ACGT 3 cut(s) 843, 2242, 2326
Hsp92I GRCGYC 2 cut(s) 1852, 2326
HspAI GCGC 4 cut(s) 303, 1936, 2251, 2653
Ksp22I TGATCA 2 cut(s) 366, 714
Kzo9I GATC 8 cut(s) 99, 366, 714, 1162, 1204, 1383, 1495, 2404
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 4 cut(s) 310, 419, 864, 1171
LweI GCATC 8 cut(s) 130, 950, 1207, 1479, 1491, 1587, 2115, 2514
MabI ACCWGGT 1 cut(s) 825
MaeII ACGT 3 cut(s) 843, 2242, 2326
MaeIII GTNAC 4 cut(s) 13, 792, 916, 1440
MalI GATC 8 cut(s) 101, 368, 716, 1164, 1206, 1385, 1497, 2406
MboI GATC 8 cut(s) 99, 366, 714, 1162, 1204, 1383, 1495, 2404
MfeI CAATTG 2 cut(s) 1271, 2157
MhlI GDGCHC 1 cut(s) 1820
MlyI GAGTC 6 cut(s) 476, 806, 1329, 1726, 1850, 2086
MmeI TCCRAC 3 cut(s) 394, 2442, 2451
Mph1103I ATGCAT 2 cut(s) 664, 1494
MroXI GAANNNNTTC 6 cut(s) 128, 438, 1107, 1902, 2217, 2628
MslI CAYNNNNRTG 1 cut(s) 249
MspCI CTTAAG 1 cut(s) 2099
MspI CCGG 3 cut(s) 1634, 1769, 2536
MspR9I CCNGG 4 cut(s) 827, 1634, 1635, 1770
MunI CAATTG 2 cut(s) 1271, 2157
Mva1269I GAATGC 1 cut(s) 769
MvaI CCWGG 1 cut(s) 827
MvnI CGCG 2 cut(s) 303, 2251
MwoI GCNNNNNNNGC 5 cut(s) 155, 1124, 1933, 2032, 2173
NciI CCSGG 3 cut(s) 1634, 1635, 1770
NdeI CATATG 1 cut(s) 2419
NdeII GATC 8 cut(s) 99, 366, 714, 1162, 1204, 1383, 1495, 2404
NmeAIII GCCGAG 1 cut(s) 1552
NsiI ATGCAT 2 cut(s) 664, 1494
NspI RCATGY 3 cut(s) 250, 1376, 1681
NspV TTCGAA 2 cut(s) 706, 2257
PceI AGGCCT 1 cut(s) 1107
PciI ACATGT 3 cut(s) 246, 1372, 1677
PciSI GCTCTTC 1 cut(s) 543
PctI GAATGC 1 cut(s) 769
PdmI GAANNNNTTC 6 cut(s) 128, 438, 1107, 1902, 2217, 2628
PfeI GAWTC 6 cut(s) 192, 388, 909, 1898, 2054, 2170
PflMI CCANNNNNTGG 1 cut(s) 1090
PleI GAGTC 6 cut(s) 475, 806, 1329, 1726, 1849, 2086
PpsI GAGTC 6 cut(s) 475, 806, 1329, 1726, 1849, 2086
PscI ACATGT 3 cut(s) 246, 1372, 1677
Psp6I CCWGG 1 cut(s) 825
PspGI CCWGG 1 cut(s) 825
PspPI GGNCC 2 cut(s) 19, 2584
PstI CTGCAG 1 cut(s) 2316
RsaI GTAC 3 cut(s) 749, 1046, 2540
RsaNI GTAC 3 cut(s) 748, 1045, 2539
RseI CAYNNNNRTG 1 cut(s) 249
SalI GTCGAC 2 cut(s) 270, 1519
SapI GCTCTTC 1 cut(s) 543
Sau3AI GATC 8 cut(s) 99, 366, 714, 1162, 1204, 1383, 1495, 2404
Sau96I GGNCC 2 cut(s) 19, 2584
SchI GAGTC 6 cut(s) 476, 806, 1329, 1726, 1850, 2086
ScrFI CCNGG 4 cut(s) 827, 1634, 1635, 1770
SduI GDGCHC 1 cut(s) 1820
SexAI ACCWGGT 1 cut(s) 825
SfaNI GCATC 8 cut(s) 130, 950, 1207, 1479, 1491, 1587, 2115, 2514
SfcI CTRYAG 2 cut(s) 1845, 2312
SfuI TTCGAA 2 cut(s) 706, 2257
SinI GGWCC 1 cut(s) 19
SmaI CCCGGG 1 cut(s) 1635
SmiI ATTTAAAT 1 cut(s) 1806
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 3 cut(s) 50, 1795, 2099
SmoI CTYRAG 3 cut(s) 50, 1795, 2099
SseBI AGGCCT 1 cut(s) 1107
SsiI CCGC 6 cut(s) 464, 775, 1432, 1858, 2249, 2443
SspI AATATT 1 cut(s) 2014
StuI AGGCCT 1 cut(s) 1107
StyD4I CCNGG 4 cut(s) 825, 1632, 1633, 1768
StyI CCWWGG 2 cut(s) 615, 2297
SwaI ATTTAAAT 1 cut(s) 1806
TaaI ACNGT 5 cut(s) 401, 565, 629, 673, 2575
TaiI ACGT 3 cut(s) 846, 2245, 2329
TatI WGTACW 1 cut(s) 1044
TfiI GAWTC 6 cut(s) 192, 388, 909, 1898, 2054, 2170
TscAI CASTG 8 cut(s) 406, 634, 1710, 1966, 2229, 2316, 2388, 2580
TspGWI ACGGA 2 cut(s) 32, 1577
TspMI CCCGGG 1 cut(s) 1633
TspRI CASTG 8 cut(s) 406, 634, 1710, 1966, 2229, 2316, 2388, 2580
Van91I CCANNNNNTGG 1 cut(s) 1090
Vha464I CTTAAG 1 cut(s) 2099
VpaK11BI GGWCC 1 cut(s) 19
XapI RAATTY 6 cut(s) 233, 757, 858, 1807, 2413, 2624
XbaI TCTAGA 4 cut(s) 835, 1201, 1735, 2345
XceI RCATGY 3 cut(s) 250, 1376, 1681
XcmI CCANNNNNNNNNTGG 3 cut(s) 315, 823, 1156
XmaI CCCGGG 1 cut(s) 1633
XmiI GTMKAC 4 cut(s) 271, 696, 1520, 1551
XmnI GAANNNNTTC 6 cut(s) 128, 438, 1107, 1902, 2217, 2628
ZraI GACGTC 1 cut(s) 2327
Zsp2I ATGCAT 2 cut(s) 664, 1494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.