MD11G1214800.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002678
Physical Location & Seq
Reverse (-)
31342572 .. 31344627
2056 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1214800.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGGCTAAATATCACACTGATGGTTTGCCAATGGCTTCCTTCGTCGTCGTCTTTTCTCTCTTCACTTCTAGTTTCATCGTCCCTCTTTCGGAAGCTCAGATGGTTCCAGCCATTTACGTGTTCGGAGACTCGTTAGTGGACGTCGGTAACAACAATTACCTAAAAATTTCTCTTGCCAAGGCAAATTTTCCTCATAATGGGGTTGATTATCCTGGCAAAGTAGCCACGGGGAGGTTTGGTAATGGCAAGAATTCTGCAGATTTTCTTGCCGAGAAAGTAGGATTACCACCGTCACCACCATACCTGTCACTTGTATCCAAGTCCAACAAGCAAAAGGCGTCATTCGTCAACGGAGTTAGCTTCGCCTCCGGAGGAGCTGGAATCTTCAATGGTTCAGATAATCAATTTCGTCAGTCAATACCTTTGTCATACCAAGTAAACTACTACTCAGAAGTATATCAAAACATTCGGCTACAGTTAGGATCCGAGGGCGCCAAAGACCATCTTTCAAAATCTCTGTTTTTGATCGTGATTGGAAGCAATGACATCTTCGACTACATTAAAAAATCCAAGCTCCAAAACGAATACACGCCGCAGCAGTACGCGGTTTTGATGGCGTCGAATCTAAAGGAACAATTACAGAGAATACATGCTCTCGGCGCCCAAAAATTTGTAGTTGTCGGGACTGGGCCGGTCGGATGCTGTCCGGCGCAAAGGGATTCAAATACAGAAGAGTGCAAAGAGGGCGCAAACTCATTGTCTGTCATGTACAATGAAGCCCTTAAGTCCATGTTGCAGCAATTGAAACCGGCGTTGGGCATAAGCTACACCCTCTTCAACACTTACACTGTCTTGACTAACATCGTGCAAAACGCAGCTTCTTACGGATTTACAGAGGTTAAAGCTGCATGCTGTGGGATTGGGAAGCTTAACGCCGAGGGTCCTTGCCTGCCAGTTGCATCCTTGTGCTCCAACAGAAGGGACCATGTCTTCTGGGATTTTTACCATCCTACACAGGCAACTCATGGCATCATAGTGGAAAAATTTTTCGATGGCCCTTCAAAATACGCATCCCCGATGACTGTGAAGGAGTTAATCGCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

369

Amino Acids

40.08

Weight (kDa)

8.51

Isoelectric Point (pI)

32.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 348 3.9e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 144
AccB1I GGYRCC 2 cut(s) 491, 659
AccII CGCG 1 cut(s) 605
AccIII TCCGGA 1 cut(s) 368
AciI CCGC 2 cut(s) 593, 605
AclWI GGATC 2 cut(s) 477, 490
AcsI RAATTY 5 cut(s) 165, 184, 250, 668, 1043
AcyI GRCGYC 5 cut(s) 141, 338, 492, 617, 660
AfaI GTAC 2 cut(s) 602, 770
AfiI CCNNNNNNNGG 5 cut(s) 88, 197, 231, 815, 978
AflII CTTAAG 1 cut(s) 782
AflIII ACRYGT 1 cut(s) 117
AgsI TTSAA 6 cut(s) 388, 510, 723, 805, 838, 1062
AjnI CCWGG 1 cut(s) 211
AjuI GAANNNNNNNTTGG 2 cut(s) 797, 829
AluBI AGCT 8 cut(s) 95, 360, 377, 574, 825, 878, 905, 928
AluI AGCT 8 cut(s) 95, 360, 377, 574, 825, 878, 905, 928
Alw21I GWGCWC 1 cut(s) 971
Alw26I GTCTC 1 cut(s) 120
AlwI GGATC 2 cut(s) 477, 490
Aor13HI TCCGGA 1 cut(s) 368
AoxI GGCC 2 cut(s) 689, 1054
ApeKI GCWGC 4 cut(s) 595, 796, 875, 905
ApoI RAATTY 5 cut(s) 165, 184, 250, 668, 1043
ArsI GACNNNNNNTTYG 2 cut(s) 743, 775
AspLEI GCGC 4 cut(s) 494, 662, 712, 749
AspS9I GGNCC 4 cut(s) 689, 941, 982, 1055
AsuHPI GGTGA 1 cut(s) 285
AvaII GGWCC 2 cut(s) 941, 982
BamHI GGATCC 1 cut(s) 482
BanI GGYRCC 2 cut(s) 491, 659
BbsI GAAGAC 1 cut(s) 982
Bbv12I GWGCWC 1 cut(s) 971
BbvI GCAGC 4 cut(s) 607, 808, 887, 892
BccI CCATC 6 cut(s) 14, 94, 510, 607, 1014, 1046
BciT130I CCWGG 1 cut(s) 213
BciVI GTATCC 1 cut(s) 325
BcoDI GTCTC 1 cut(s) 120
BfaI CTAG 1 cut(s) 69
BfmI CTRYAG 2 cut(s) 255, 473
BfoI RGCGCY 2 cut(s) 495, 663
BfrI CTTAAG 1 cut(s) 782
BfuI GTATCC 1 cut(s) 325
BisI GCNGC 5 cut(s) 593, 596, 797, 876, 906
BlsI GCNGC 5 cut(s) 594, 597, 798, 877, 907
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 2 cut(s) 941, 982
BmgT120I GGNCC 4 cut(s) 689, 941, 982, 1055
BmiI GGNNCC 6 cut(s) 105, 484, 493, 661, 942, 983
BmrFI CCNGG 1 cut(s) 213
BmrI ACTGGG 1 cut(s) 696
BmsI GCATC 4 cut(s) 689, 968, 1038, 1079
BmuI ACTGGG 1 cut(s) 696
BpiI GAAGAC 1 cut(s) 982
BsaAI YACGTR 1 cut(s) 118
BsaHI GRCGYC 5 cut(s) 141, 338, 492, 617, 660
BsaJI CCNNGG 4 cut(s) 177, 225, 486, 936
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 2 cut(s) 223, 253
Bsc4I CCNNNNNNNGG 5 cut(s) 88, 197, 231, 815, 978
Bse118I RCCGGY 2 cut(s) 691, 808
Bse1I ACTGG 2 cut(s) 691, 953
Bse3DI GCAATG 1 cut(s) 547
BseAI TCCGGA 1 cut(s) 368
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 4 cut(s) 177, 225, 486, 936
BseGI GGATG 4 cut(s) 704, 959, 1006, 1070
BseLI CCNNNNNNNGG 5 cut(s) 88, 197, 231, 815, 978
BseMI GCAATG 1 cut(s) 547
BseMII CTCAG 2 cut(s) 110, 462
BseNI ACTGG 2 cut(s) 691, 953
BseRI GAGGAG 1 cut(s) 387
BseXI GCAGC 4 cut(s) 607, 808, 887, 892
Bsh1236I CGCG 1 cut(s) 605
Bsh1285I CGRYCG 1 cut(s) 696
BshFI GGCC 2 cut(s) 691, 1056
BshNI GGYRCC 2 cut(s) 491, 659
BsiEI CGRYCG 1 cut(s) 696
BsiHKAI GWGCWC 1 cut(s) 971
BsiSI CCGG 4 cut(s) 369, 692, 707, 809
BslFI GGGAC 3 cut(s) 65, 697, 995
BslI CCNNNNNNNGG 5 cut(s) 88, 197, 231, 815, 978
BsmAI GTCTC 1 cut(s) 120
BsmFI GGGAC 3 cut(s) 65, 697, 995
BsnI GGCC 2 cut(s) 691, 1056
Bsp1286I GDGCHC 1 cut(s) 971
Bsp13I TCCGGA 1 cut(s) 368
Bsp1407I TGTACA 1 cut(s) 768
Bsp143I GATC 2 cut(s) 482, 525
BspACI CCGC 2 cut(s) 593, 605
BspANI GGCC 2 cut(s) 691, 1056
BspCNI CTCAG 2 cut(s) 109, 461
BspEI TCCGGA 1 cut(s) 368
BspFNI CGCG 1 cut(s) 605
BspLI GGNNCC 6 cut(s) 105, 484, 493, 661, 942, 983
BspMAI CTGCAG 1 cut(s) 259
BspPI GGATC 2 cut(s) 477, 490
BspT107I GGYRCC 2 cut(s) 491, 659
BspTI CTTAAG 1 cut(s) 782
BsrDI GCAATG 1 cut(s) 547
BsrFI RCCGGY 2 cut(s) 691, 808
BsrGI TGTACA 1 cut(s) 768
BsrI ACTGG 2 cut(s) 691, 953
BssAI RCCGGY 2 cut(s) 691, 808
BssECI CCNNGG 4 cut(s) 177, 225, 486, 936
BssMI GATC 2 cut(s) 482, 525
BssNI GRCGYC 5 cut(s) 141, 338, 492, 617, 660
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 4 cut(s) 291, 477, 850, 1084
Bst6I CTCTTC 3 cut(s) 65, 726, 839
BstACI GRCGYC 5 cut(s) 141, 338, 492, 617, 660
BstAFI CTTAAG 1 cut(s) 782
BstAUI TGTACA 1 cut(s) 768
BstBAI YACGTR 1 cut(s) 118
BstC8I GCNNGC 2 cut(s) 910, 950
BstDEI CTNAG 2 cut(s) 96, 448
BstDSI CCRYGG 1 cut(s) 225
BstF5I GGATG 4 cut(s) 704, 959, 1006, 1070
BstFNI CGCG 1 cut(s) 605
BstH2I RGCGCY 2 cut(s) 495, 663
BstHHI GCGC 4 cut(s) 494, 662, 712, 749
BstKTI GATC 2 cut(s) 485, 528
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 2 cut(s) 482, 525
BstMCI CGRYCG 1 cut(s) 696
BstMWI GCNNNNNNNGC 2 cut(s) 659, 744
BstNI CCWGG 1 cut(s) 213
BstNSI RCATGY 2 cut(s) 653, 912
BstSCI CCNGG 1 cut(s) 211
BstSFI CTRYAG 2 cut(s) 255, 473
BstUI CGCG 1 cut(s) 605
BstV1I GCAGC 4 cut(s) 607, 808, 887, 892
BstV2I GAAGAC 1 cut(s) 982
BstX2I RGATCY 1 cut(s) 482
BstYI RGATCY 1 cut(s) 482
BsuI GTATCC 1 cut(s) 325
BsuRI GGCC 2 cut(s) 691, 1056
BtgI CCRYGG 1 cut(s) 225
BtsCI GGATG 4 cut(s) 704, 959, 1006, 1070
BtsIMutI CAGTG 2 cut(s) 15, 846
Cac8I GCNNGC 2 cut(s) 910, 950
CfoI GCGC 4 cut(s) 494, 662, 712, 749
Cfr10I RCCGGY 2 cut(s) 691, 808
Cfr13I GGNCC 4 cut(s) 689, 941, 982, 1055
CseI GACGC 2 cut(s) 327, 606
Csp6I GTAC 2 cut(s) 601, 769
CviAII CATG 6 cut(s) 650, 766, 790, 909, 986, 1025
CviQI GTAC 2 cut(s) 601, 769
DdeI CTNAG 2 cut(s) 96, 448
DinI GGCGCC 2 cut(s) 493, 661
DpnI GATC 2 cut(s) 484, 527
DpnII GATC 2 cut(s) 482, 525
Eam1104I CTCTTC 3 cut(s) 65, 726, 839
EarI CTCTTC 3 cut(s) 65, 726, 839
Eco130I CCWWGG 1 cut(s) 177
Eco47I GGWCC 2 cut(s) 941, 982
EcoO109I RGGNCCY 1 cut(s) 941
EcoRI GAATTC 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 177
EgeI GGCGCC 2 cut(s) 493, 661
EheI GGCGCC 2 cut(s) 493, 661
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 6 cut(s) 653, 769, 793, 912, 989, 1028
FalI AAGNNNNNCTT 2 cut(s) 489, 521
FaqI GGGAC 3 cut(s) 65, 697, 995
FatI CATG 6 cut(s) 649, 765, 789, 908, 985, 1024
Fnu4HI GCNGC 5 cut(s) 593, 596, 797, 876, 906
FokI GGATG 4 cut(s) 711, 946, 993, 1057
Fsp4HI GCNGC 5 cut(s) 593, 596, 797, 876, 906
FspBI CTAG 1 cut(s) 69
GlaI GCGC 4 cut(s) 493, 661, 711, 748
GluI GCNGC 5 cut(s) 593, 596, 797, 876, 906
HaeII RGCGCY 2 cut(s) 495, 663
HaeIII GGCC 2 cut(s) 691, 1056
HapII CCGG 4 cut(s) 369, 692, 707, 809
HgaI GACGC 2 cut(s) 327, 606
HhaI GCGC 4 cut(s) 494, 662, 712, 749
Hin1I GRCGYC 5 cut(s) 141, 338, 492, 617, 660
Hin1II CATG 6 cut(s) 653, 769, 793, 912, 989, 1028
Hin6I GCGC 4 cut(s) 492, 660, 710, 747
HinP1I GCGC 4 cut(s) 492, 660, 710, 747
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 926
HinfI GANTC 4 cut(s) 128, 381, 622, 719
HpaII CCGG 4 cut(s) 369, 692, 707, 809
HphI GGTGA 1 cut(s) 285
Hpy166II GTNNAC 3 cut(s) 139, 349, 439
Hpy188I TCNGA 7 cut(s) 91, 99, 125, 397, 451, 487, 698
Hpy188III TCNNGA 4 cut(s) 369, 529, 682, 853
Hpy8I GTNNAC 3 cut(s) 139, 349, 439
Hpy99I CGWCG 4 cut(s) 47, 50, 146, 622
HpyAV CCTTC 4 cut(s) 49, 972, 1068, 1081
HpyCH4III ACNGT 4 cut(s) 291, 477, 850, 1084
HpyCH4IV ACGT 2 cut(s) 117, 141
HpyCH4V TGCA 6 cut(s) 257, 738, 796, 868, 908, 959
HpyF10VI GCNNNNNNNGC 2 cut(s) 659, 744
HpyF3I CTNAG 2 cut(s) 96, 448
HpySE526I ACGT 2 cut(s) 117, 141
Hsp92I GRCGYC 5 cut(s) 141, 338, 492, 617, 660
Hsp92II CATG 6 cut(s) 653, 769, 793, 912, 989, 1028
HspAI GCGC 4 cut(s) 492, 660, 710, 747
KasI GGCGCC 2 cut(s) 491, 659
Kpn2I TCCGGA 1 cut(s) 368
Kzo9I GATC 2 cut(s) 482, 525
LmnI GCTCC 3 cut(s) 374, 579, 974
Lsp1109I GCAGC 4 cut(s) 607, 808, 887, 892
LweI GCATC 4 cut(s) 689, 968, 1038, 1079
MaeI CTAG 1 cut(s) 69
MaeII ACGT 2 cut(s) 117, 141
MaeIII GTNAC 3 cut(s) 146, 291, 306
MalI GATC 2 cut(s) 484, 527
MboI GATC 2 cut(s) 482, 525
MboII GAAGA 6 cut(s) 52, 376, 541, 743, 826, 982
MfeI CAATTG 1 cut(s) 800
MflI RGATCY 1 cut(s) 482
MhlI GDGCHC 1 cut(s) 971
MluCI AATT 9 cut(s) 154, 165, 184, 250, 404, 635, 668, 800, 1043
Mly113I GGCGCC 2 cut(s) 492, 660
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 3 cut(s) 348, 676, 996
MroI TCCGGA 1 cut(s) 368
MseI TTAA 6 cut(s) 561, 783, 900, 930, 1094, 1105
MslI CAYNNNNRTG 4 cut(s) 18, 116, 964, 1034
MspCI CTTAAG 1 cut(s) 782
MspI CCGG 4 cut(s) 369, 692, 707, 809
MspR9I CCNGG 1 cut(s) 213
MunI CAATTG 1 cut(s) 800
MvaI CCWGG 1 cut(s) 213
MvnI CGCG 1 cut(s) 605
MwoI GCNNNNNNNGC 2 cut(s) 659, 744
NarI GGCGCC 2 cut(s) 492, 660
NdeII GATC 2 cut(s) 482, 525
NlaIII CATG 6 cut(s) 653, 769, 793, 912, 989, 1028
NlaIV GGNNCC 6 cut(s) 105, 484, 493, 661, 942, 983
NmeAIII GCCGAG 3 cut(s) 295, 636, 961
NmuCI GTSAC 2 cut(s) 291, 306
NspI RCATGY 2 cut(s) 653, 912
PaeI GCATGC 1 cut(s) 912
PfeI GAWTC 3 cut(s) 381, 622, 719
PflFI GACNNNGTC 1 cut(s) 986
PkrI GCNGC 5 cut(s) 594, 597, 798, 877, 907
PleI GAGTC 1 cut(s) 122
PluTI GGCGCC 2 cut(s) 495, 663
PpsI GAGTC 1 cut(s) 122
Ppu21I YACGTR 1 cut(s) 118
PpuMI RGGWCCY 1 cut(s) 941
Psp5II RGGWCCY 1 cut(s) 941
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 6 cut(s) 105, 484, 493, 661, 942, 983
PspPI GGNCC 4 cut(s) 689, 941, 982, 1055
PspPPI RGGWCCY 1 cut(s) 941
PstI CTGCAG 1 cut(s) 259
PsuI RGATCY 1 cut(s) 482
PsyI GACNNNGTC 1 cut(s) 986
RsaI GTAC 2 cut(s) 602, 770
RsaNI GTAC 2 cut(s) 601, 769
RseI CAYNNNNRTG 4 cut(s) 18, 116, 964, 1034
SaqAI TTAA 6 cut(s) 561, 783, 900, 930, 1094, 1105
SatI GCNGC 5 cut(s) 593, 596, 797, 876, 906
Sau3AI GATC 2 cut(s) 482, 525
Sau96I GGNCC 4 cut(s) 689, 941, 982, 1055
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 1 cut(s) 971
SfaNI GCATC 4 cut(s) 689, 968, 1038, 1079
SfcI CTRYAG 2 cut(s) 255, 473
SfoI GGCGCC 2 cut(s) 493, 661
SinI GGWCC 2 cut(s) 941, 982
SmiMI CAYNNNNRTG 4 cut(s) 18, 116, 964, 1034
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
SphI GCATGC 1 cut(s) 912
Sse9I AATT 9 cut(s) 154, 165, 184, 250, 404, 635, 668, 800, 1043
SsiI CCGC 2 cut(s) 593, 605
SspDI GGCGCC 2 cut(s) 491, 659
SspMI CTAG 1 cut(s) 69
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 4 cut(s) 291, 477, 850, 1084
TaiI ACGT 2 cut(s) 120, 144
TaqI TCGA 3 cut(s) 552, 620, 1050
TasI AATT 9 cut(s) 154, 165, 184, 250, 404, 635, 668, 800, 1043
TatI WGTACW 1 cut(s) 768
TauI GCSGC 1 cut(s) 595
TfiI GAWTC 3 cut(s) 381, 622, 719
Tru1I TTAA 6 cut(s) 561, 783, 900, 930, 1094, 1105
Tru9I TTAA 6 cut(s) 561, 783, 900, 930, 1094, 1105
TscAI CASTG 2 cut(s) 22, 853
TseFI GTSAC 2 cut(s) 291, 306
TseI GCWGC 4 cut(s) 595, 796, 875, 905
Tsp45I GTSAC 2 cut(s) 291, 306
TspDTI ATGAA 2 cut(s) 64, 789
TspGWI ACGGA 2 cut(s) 366, 900
TspRI CASTG 2 cut(s) 22, 853
Tth111I GACNNNGTC 1 cut(s) 986
Vha464I CTTAAG 1 cut(s) 782
VpaK11BI GGWCC 2 cut(s) 941, 982
XapI RAATTY 5 cut(s) 165, 184, 250, 668, 1043
XceI RCATGY 2 cut(s) 653, 912
XspI CTAG 1 cut(s) 69
ZraI GACGTC 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.