Rh5BG229800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002678
Physical Location & Seq
Forward (+)
27000069 .. 27002786
2718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG229800.1

Sequence Viewer

Length: 1311 bp
ATGATCGAATTTATGATGGAGGGAGCCCCAGATGACCAGTCCTCCAGTCAAATTATACTGTCTTTTGACTCTTCTCAGTTTTCACACACAAAAGGGCACAGACACACTCATTTACTATCCTCTTTATGCATAAACCAAACTCACCACCGCCTCACAGAGGCTGCAAATAGAAATAGCATAGTGTTGGCTGTGACTGAGATCACAAACTTCATCTATACAATGGCCTGCAATTCCTTCCTTCCAAGTTTCTGCGTCTTCTTGCTTTTCTTGCTGTACGGGGTTTTGTATACAGTTACGGAGGCTCGTGTGATTCCGGCCATGTTTGTTTTCGGAGACTCCCTAGTAGATGTAGGCAACAACAATTACCTCAGGTATTCATTTGCCAAAGCAAACTTTCCTCACAATGGGGTCGACTTTCCGACCAGAAAACCAACCGGCAGGTTCGGCAATGGCAAGAATGCTGCAGATCTTATTGCTGAGAAAATGGGGTTGCCGACAATACCACCGTATCTCTCCATGTCATCCAAATCAAACAAGAGCATTACACAGTTCCTAAACGGCGTTAACTTTGCATCTGGAGCTTCCAAAATCTTAAACGACATAGATCCACAATATCCCTTTTCCATACCTTTGGAAAAACAAGTAGACTACTATTTGGCAGTGCATAAAGACCTAGCGCAAGGGCTAGGAGCCTCCAGAGCACAAATTTATTTATCAAAATCTCTCTTCCTCATTATCACGGGAAGCAATGACATCTACAACTACTTTGACTCGTTAAGTAACAGCACACAACAGCAGTACGTGAACTCCATTGTTCTCATGTTCAAAGAACAAGTGAAGCGGCTGTATGATTATGGTGCACGCAAATTCTCGATTGTTGGGGTTGGGGTTATCGGATGCACACCATCAGAAAGGAATGAGCAGGCGGACAGAAAATGCAATGAAGATACAAATCGATTGTCACTCAAGTACAATCAAGCACTCGTGTCCATGTTGAAGAATTTGGCATCAGAGCTCAGAGGCATAAACTACTCCTACTTTGATGGTTACAGCGTCATGCAGAACTTCATCCAAAAACCAACAGCTTATGGATTTTCTGAGGTTAAAGCTGCTTGCTGTGGTCTTGGGAAGCTCAATGCCGATGCTCCTTGCCTGCCATTTGCGACTTACTGCACCAACAGAAGCAACCATCTGTTCTGGGACAGAACGCATCCTACTGAGGCAGCTCATCGCAAGCTTGTGGATTATATGCTTTATGGTCCTTTACAATACACATTTCCACTCAATGTGAAAAAGCTAGCTGCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

436

Amino Acids

48.87

Weight (kDa)

8.68

Isoelectric Point (pI)

28.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 108 - 416 9.8e-36 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 429
AccI GTMKAC 3 cut(s) 287, 411, 645
AciI CCGC 3 cut(s) 148, 841, 926
AclWI GGATC 1 cut(s) 599
AcoI YGGCCR 1 cut(s) 315
AcsI RAATTY 4 cut(s) 8, 705, 866, 1000
AfaI GTAC 3 cut(s) 275, 800, 971
AfiI CCNNNNNNNGG 2 cut(s) 157, 404
AgsI TTSAA 2 cut(s) 826, 997
AluBI AGCT 9 cut(s) 581, 1015, 1085, 1109, 1132, 1226, 1237, 1297, 1301
AluI AGCT 9 cut(s) 581, 1015, 1085, 1109, 1132, 1226, 1237, 1297, 1301
Alw21I GWGCWC 3 cut(s) 703, 862, 1017
Alw26I GTCTC 1 cut(s) 327
Alw44I GTGCAC 1 cut(s) 858
AlwI GGATC 1 cut(s) 599
AlwNI CAGNNNCTG 1 cut(s) 161
AoxI GGCC 2 cut(s) 222, 315
ApaLI GTGCAC 1 cut(s) 858
ApeKI GCWGC 5 cut(s) 161, 461, 1109, 1223, 1301
ApoI RAATTY 4 cut(s) 8, 705, 866, 1000
ArsI GACNNNNNNTTYG 2 cut(s) 944, 976
AspLEI GCGC 1 cut(s) 679
AspS9I GGNCC 1 cut(s) 1259
AsuHPI GGTGA 1 cut(s) 134
AsuNHI GCTAGC 1 cut(s) 1297
AvaII GGWCC 1 cut(s) 1259
AxyI CCTNAGG 1 cut(s) 368
BaeGI GKGCMC 2 cut(s) 99, 862
BaeI ACNNNNGTAYC 2 cut(s) 491, 524
BanII GRGCYC 2 cut(s) 28, 1017
BauI CACGAG 2 cut(s) 303, 983
BbsI GAAGAC 1 cut(s) 247
Bbv12I GWGCWC 3 cut(s) 703, 862, 1017
BbvI GCAGC 5 cut(s) 148, 448, 1096, 1235, 1288
BccI CCATC 4 cut(s) 10, 913, 1037, 1197
BceAI ACGGC 1 cut(s) 574
BcoDI GTCTC 1 cut(s) 327
BfaI CTAG 4 cut(s) 341, 674, 686, 1298
BfmI CTRYAG 1 cut(s) 462
BfuAI ACCTGC 1 cut(s) 429
BglII AGATCT 1 cut(s) 466
BisI GCNGC 6 cut(s) 162, 462, 842, 1110, 1224, 1302
BlsI GCNGC 6 cut(s) 163, 463, 843, 1111, 1225, 1303
Bme18I GGWCC 1 cut(s) 1259
BmgT120I GGNCC 1 cut(s) 1259
BmiI GGNNCC 2 cut(s) 25, 691
BmsI GCATC 5 cut(s) 581, 887, 1016, 1132, 1219
BmtI GCTAGC 1 cut(s) 1301
BpiI GAAGAC 1 cut(s) 247
BpmI CTGGAG 3 cut(s) 28, 597, 679
BpuEI CTTGAG 1 cut(s) 950
Bsa29I ATCGAT 1 cut(s) 955
BsaAI YACGTR 1 cut(s) 802
BsaBI GATNNNNATC 1 cut(s) 951
BsaXI ACNNNNNCTCC 4 cut(s) 26, 56, 791, 821
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 404
Bse118I RCCGGY 1 cut(s) 434
Bse1I ACTGG 2 cut(s) 37, 45
Bse21I CCTNAGG 1 cut(s) 368
Bse3DI GCAATG 3 cut(s) 454, 754, 946
Bse8I GATNNNNATC 1 cut(s) 951
BseCI ATCGAT 1 cut(s) 955
BseGI GGATG 4 cut(s) 521, 902, 1068, 1210
BseJI GATNNNNATC 1 cut(s) 951
BseLI CCNNNNNNNGG 2 cut(s) 157, 404
BseMI GCAATG 3 cut(s) 454, 754, 946
BseMII CTCAG 7 cut(s) 89, 186, 382, 468, 1030, 1089, 1209
BseNI ACTGG 2 cut(s) 37, 45
BseSI GKGCMC 2 cut(s) 99, 862
BseXI GCAGC 5 cut(s) 148, 448, 1096, 1235, 1288
BsgI GTGCAG 1 cut(s) 1156
BshFI GGCC 2 cut(s) 224, 317
BshVI ATCGAT 1 cut(s) 955
BsiHKAI GWGCWC 3 cut(s) 703, 862, 1017
BsiSI CCGG 2 cut(s) 314, 435
BslFI GGGAC 1 cut(s) 1214
BslI CCNNNNNNNGG 2 cut(s) 157, 404
BsmAI GTCTC 1 cut(s) 327
BsmFI GGGAC 1 cut(s) 1214
BsmI GAATGC 1 cut(s) 463
BsnI GGCC 2 cut(s) 224, 317
Bsp1286I GDGCHC 5 cut(s) 28, 99, 703, 862, 1017
Bsp143I GATC 4 cut(s) 3, 198, 466, 604
BspACI CCGC 3 cut(s) 148, 841, 926
BspANI GGCC 2 cut(s) 224, 317
BspCNI CTCAG 7 cut(s) 88, 187, 381, 469, 1029, 1090, 1210
BspDI ATCGAT 1 cut(s) 955
BspLI GGNNCC 2 cut(s) 25, 691
BspMAI CTGCAG 1 cut(s) 466
BspMI ACCTGC 1 cut(s) 429
BspOI GCTAGC 1 cut(s) 1301
BspPI GGATC 1 cut(s) 599
BsrDI GCAATG 3 cut(s) 454, 754, 946
BsrFI RCCGGY 1 cut(s) 434
BsrI ACTGG 2 cut(s) 37, 45
BssAI RCCGGY 1 cut(s) 434
BssMI GATC 4 cut(s) 3, 198, 466, 604
BssNAI GTATAC 1 cut(s) 288
BssSI CACGAG 2 cut(s) 303, 983
Bst1107I GTATAC 1 cut(s) 288
Bst2BI CACGAG 2 cut(s) 303, 983
Bst4CI ACNGT 4 cut(s) 60, 292, 507, 549
Bst6I CTCTTC 2 cut(s) 76, 731
BstBAI YACGTR 1 cut(s) 802
BstC8I GCNNGC 7 cut(s) 226, 862, 924, 1114, 1154, 1235, 1299
BstDEI CTNAG 7 cut(s) 75, 195, 368, 477, 1016, 1098, 1218
BstENI CCTNNNNNAGG 1 cut(s) 155
BstF5I GGATG 4 cut(s) 521, 902, 1068, 1210
BstHHI GCGC 1 cut(s) 679
BstKTI GATC 4 cut(s) 6, 201, 469, 607
BstMAI GTCTC 1 cut(s) 327
BstMBI GATC 4 cut(s) 3, 198, 466, 604
BstMWI GCNNNNNNNGC 4 cut(s) 268, 444, 578, 698
BstSFI CTRYAG 1 cut(s) 462
BstSLI GKGCMC 2 cut(s) 99, 862
BstV1I GCAGC 5 cut(s) 148, 448, 1096, 1235, 1288
BstV2I GAAGAC 1 cut(s) 247
BstX2I RGATCY 2 cut(s) 466, 604
BstXI CCANNNNNNTGG 1 cut(s) 631
BstYI RGATCY 2 cut(s) 466, 604
BstZ17I GTATAC 1 cut(s) 288
Bsu15I ATCGAT 1 cut(s) 955
Bsu36I CCTNAGG 1 cut(s) 368
BsuRI GGCC 2 cut(s) 224, 317
BsuTUI ATCGAT 1 cut(s) 955
BtgZI GCGATG 1 cut(s) 1214
BtsCI GGATG 4 cut(s) 521, 902, 1068, 1210
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 1 cut(s) 666
BveI ACCTGC 1 cut(s) 429
Cac8I GCNNGC 7 cut(s) 226, 862, 924, 1114, 1154, 1235, 1299
CaiI CAGNNNCTG 1 cut(s) 161
CfoI GCGC 1 cut(s) 679
Cfr10I RCCGGY 1 cut(s) 434
Cfr13I GGNCC 1 cut(s) 1259
ClaI ATCGAT 1 cut(s) 955
CseI GACGC 2 cut(s) 241, 1042
Csp6I GTAC 3 cut(s) 274, 799, 970
CviAII CATG 5 cut(s) 319, 517, 820, 991, 1057
CviQI GTAC 3 cut(s) 274, 799, 970
DdeI CTNAG 7 cut(s) 75, 195, 368, 477, 1016, 1098, 1218
DpnI GATC 4 cut(s) 5, 200, 468, 606
DpnII GATC 4 cut(s) 3, 198, 466, 604
EaeI YGGCCR 1 cut(s) 315
Eam1104I CTCTTC 2 cut(s) 76, 731
EarI CTCTTC 2 cut(s) 76, 731
EciI GGCGGA 1 cut(s) 941
Ecl136II GAGCTC 1 cut(s) 1015
Eco24I GRGCYC 2 cut(s) 28, 1017
Eco47I GGWCC 1 cut(s) 1259
Eco53kI GAGCTC 1 cut(s) 1015
Eco81I CCTNAGG 1 cut(s) 368
EcoICRI GAGCTC 1 cut(s) 1015
EcoNI CCTNNNNNAGG 1 cut(s) 155
EcoT22I ATGCAT 1 cut(s) 131
EcoT38I GRGCYC 2 cut(s) 28, 1017
FaeI CATG 5 cut(s) 322, 520, 823, 994, 1060
FaqI GGGAC 1 cut(s) 1214
FatI CATG 5 cut(s) 318, 516, 819, 990, 1056
FblI GTMKAC 3 cut(s) 287, 411, 645
Fnu4HI GCNGC 6 cut(s) 162, 462, 842, 1110, 1224, 1302
FokI GGATG 4 cut(s) 508, 909, 1055, 1197
FriOI GRGCYC 2 cut(s) 28, 1017
Fsp4HI GCNGC 6 cut(s) 162, 462, 842, 1110, 1224, 1302
FspBI CTAG 4 cut(s) 341, 674, 686, 1298
GlaI GCGC 1 cut(s) 678
GluI GCNGC 6 cut(s) 162, 462, 842, 1110, 1224, 1302
GsuI CTGGAG 3 cut(s) 28, 597, 679
HaeIII GGCC 2 cut(s) 224, 317
HapII CCGG 2 cut(s) 314, 435
HgaI GACGC 2 cut(s) 241, 1042
HhaI GCGC 1 cut(s) 679
Hin1II CATG 5 cut(s) 322, 520, 823, 994, 1060
Hin6I GCGC 1 cut(s) 677
HinP1I GCGC 1 cut(s) 677
HincII GTYRAC 2 cut(s) 412, 565
HindII GTYRAC 2 cut(s) 412, 565
HindIII AAGCTT 1 cut(s) 1235
HinfI GANTC 4 cut(s) 68, 310, 335, 770
HpaI GTTAAC 1 cut(s) 565
HpaII CCGG 2 cut(s) 314, 435
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 6 cut(s) 288, 412, 565, 646, 805, 860
Hpy188I TCNGA 7 cut(s) 332, 420, 896, 910, 1012, 1019, 1099
Hpy188III TCNNGA 3 cut(s) 576, 696, 871
Hpy8I GTNNAC 6 cut(s) 288, 412, 565, 646, 805, 860
HpyAV CCTTC 2 cut(s) 244, 248
HpyCH4III ACNGT 4 cut(s) 60, 292, 507, 549
HpyCH4IV ACGT 1 cut(s) 801
HpyF10VI GCNNNNNNNGC 4 cut(s) 268, 444, 578, 698
HpyF3I CTNAG 7 cut(s) 75, 195, 368, 477, 1016, 1098, 1218
HpySE526I ACGT 1 cut(s) 801
Hsp92II CATG 5 cut(s) 322, 520, 823, 994, 1060
HspAI GCGC 1 cut(s) 677
KspAI GTTAAC 1 cut(s) 565
Kzo9I GATC 4 cut(s) 3, 198, 466, 604
LmnI GCTCC 4 cut(s) 23, 578, 689, 1150
Lsp1109I GCAGC 5 cut(s) 148, 448, 1096, 1235, 1288
LweI GCATC 5 cut(s) 581, 887, 1016, 1132, 1219
MaeI CTAG 4 cut(s) 341, 674, 686, 1298
MaeII ACGT 1 cut(s) 801
MaeIII GTNAC 5 cut(s) 190, 292, 779, 960, 1046
MalI GATC 4 cut(s) 5, 200, 468, 606
MboI GATC 4 cut(s) 3, 198, 466, 604
MboII GAAGA 5 cut(s) 63, 247, 718, 956, 1009
MflI RGATCY 2 cut(s) 466, 604
MhlI GDGCHC 5 cut(s) 28, 99, 703, 862, 1017
MluCI AATT 7 cut(s) 8, 51, 229, 361, 705, 866, 1000
MlyI GAGTC 3 cut(s) 62, 329, 764
MmeI TCCRAC 1 cut(s) 443
Mph1103I ATGCAT 1 cut(s) 131
MseI TTAA 4 cut(s) 564, 593, 776, 1104
MspI CCGG 2 cut(s) 314, 435
Mva1269I GAATGC 1 cut(s) 463
MwoI GCNNNNNNNGC 4 cut(s) 268, 444, 578, 698
NdeII GATC 4 cut(s) 3, 198, 466, 604
NheI GCTAGC 1 cut(s) 1297
NlaIII CATG 5 cut(s) 322, 520, 823, 994, 1060
NlaIV GGNNCC 2 cut(s) 25, 691
NmuCI GTSAC 2 cut(s) 190, 960
NsiI ATGCAT 1 cut(s) 131
PctI GAATGC 1 cut(s) 463
PfeI GAWTC 1 cut(s) 310
PkrI GCNGC 6 cut(s) 163, 463, 843, 1111, 1225, 1303
PleI GAGTC 3 cut(s) 62, 329, 764
PpsI GAGTC 3 cut(s) 62, 329, 764
Ppu21I YACGTR 1 cut(s) 802
Psp124BI GAGCTC 1 cut(s) 1017
PspN4I GGNNCC 2 cut(s) 25, 691
PspPI GGNCC 1 cut(s) 1259
PsrI GAACNNNNNNTAC 2 cut(s) 1198, 1230
PstI CTGCAG 1 cut(s) 466
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 2 cut(s) 466, 604
RsaI GTAC 3 cut(s) 275, 800, 971
RsaNI GTAC 3 cut(s) 274, 799, 970
SacI GAGCTC 1 cut(s) 1017
SalI GTCGAC 1 cut(s) 410
SaqAI TTAA 4 cut(s) 564, 593, 776, 1104
SatI GCNGC 6 cut(s) 162, 462, 842, 1110, 1224, 1302
Sau3AI GATC 4 cut(s) 3, 198, 466, 604
Sau96I GGNCC 1 cut(s) 1259
SchI GAGTC 3 cut(s) 62, 329, 764
SduI GDGCHC 5 cut(s) 28, 99, 703, 862, 1017
SfaNI GCATC 5 cut(s) 581, 887, 1016, 1132, 1219
SfcI CTRYAG 1 cut(s) 462
SinI GGWCC 1 cut(s) 1259
SmlI CTYRAG 1 cut(s) 965
SmoI CTYRAG 1 cut(s) 965
Sse9I AATT 7 cut(s) 8, 51, 229, 361, 705, 866, 1000
SsiI CCGC 3 cut(s) 148, 841, 926
SspMI CTAG 4 cut(s) 341, 674, 686, 1298
SstI GAGCTC 1 cut(s) 1017
TaaI ACNGT 4 cut(s) 60, 292, 507, 549
TaiI ACGT 1 cut(s) 804
TaqI TCGA 4 cut(s) 6, 411, 872, 955
TasI AATT 7 cut(s) 8, 51, 229, 361, 705, 866, 1000
TatI WGTACW 1 cut(s) 969
TauI GCSGC 1 cut(s) 844
TfiI GAWTC 1 cut(s) 310
Tru1I TTAA 4 cut(s) 564, 593, 776, 1104
Tru9I TTAA 4 cut(s) 564, 593, 776, 1104
TscAI CASTG 1 cut(s) 666
TseFI GTSAC 2 cut(s) 190, 960
TseI GCWGC 5 cut(s) 161, 461, 1109, 1223, 1301
Tsp45I GTSAC 2 cut(s) 190, 960
TspDTI ATGAA 4 cut(s) 199, 366, 957, 1057
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 1 cut(s) 666
VneI GTGCAC 1 cut(s) 858
VpaK11BI GGWCC 1 cut(s) 1259
XagI CCTNNNNNAGG 1 cut(s) 155
XapI RAATTY 4 cut(s) 8, 705, 866, 1000
XmiI GTMKAC 3 cut(s) 287, 411, 645
XspI CTAG 4 cut(s) 341, 674, 686, 1298
Zsp2I ATGCAT 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.