Rh5AG228800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002678
Physical Location & Seq
Forward (+)
27499585 .. 27501818
2234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG228800.1

Sequence Viewer

Length: 1098 bp
ATGACCATGGAAGCTTGCAGTGTTTCTCCTACGATTTTCTTCACCATCTTCTCCATTATCGTTTCTGTGTTCATCGCTTTCTCAAATGCTCAGACGGTTCCAGCCATTTACATATTTGGAGACTCTCTATTGGATGTTGGTAACAACAATTACCTTACCATCTCTGTGGCCAAGGCAAATTTTCCTCACAACGGTATCGATTTTCCGACCAAGAAACCAACCGGGAGGTTTTGCAATGGCAAGAATTCTGCAGATTTTCTGGCTGAAAAAGTAGGATTGCCAACTTCCCCACCATATCTGTCGCTTGTATCAAAATCTAATAAGCAAAAGGGGTCCTTCGAAGCTGGAGTAAGCTTCGCCTCTGGAGGGGCTGGAATCTTCAACGGCACCGATGATGAATATCGTCAGTCACTACCTTTGACAAAACAAATAGGCTACTATTCACAAGTGTGTGAAGAACTTCGGCAACAATTAGGAGCTCAAGCTGCCCAAGACCATCTATCGAAATCTCTACATGTCGTTCTTATTGGAAGCAATGACGTTTTCGGCTACTTAGATTCATCCAGCACCCAAAAGCAATACACACCGGACCAGTATGTGGATTTGATGCTGTCTACTCTTAAACAACACTTAAAGAGAATACACAGTCTTGGTGCACGAAAATTTGTAATTCTCGGGACTGGACCCGTTGGATGTTGTCCAGCACGAAGGGATGATAAAACAGGAGAGTGCAATGAAGTAGGAAACTCCATCTCTGCAAAGTATAGTGATGGCCTCAAGTCAATGATGCAGCAATTGAAACCGGCACTGGGCATAAACTACTCCTTCTTCGAGACTTATGGCATCTTCCGTAACATCGTCCAGAATCCATCAACTTACGGATTTAATGAGACTAAAGCTGCATGCTGTGGCCTCGGGTTTCTTAACGCAAAGGCTCCTTGTATACCAGTTTCATCCTACTGCTCCAATAGAAGGAACCATATGTTCTGGGATTTTTACCATCCTACAGAGGCAACTCACAAAATCTTGGTGGAACATCTTTTTGAGGGTTCCACATACACAGTCCCAATGAATGTGAAGAATTTAGTTTCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

365

Amino Acids

40.07

Weight (kDa)

8.42

Isoelectric Point (pI)

32.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 347 4.2e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 386
AccB7I CCANNNNNTGG 1 cut(s) 598
AccI GTMKAC 2 cut(s) 614, 943
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 4 cut(s) 178, 244, 662, 1081
AfiI CCNNNNNNNGG 5 cut(s) 191, 366, 598, 809, 972
AflIII ACRYGT 1 cut(s) 514
AgsI TTSAA 2 cut(s) 382, 799
AleI CACNNNNGTG 1 cut(s) 448
AluBI AGCT 6 cut(s) 14, 344, 354, 479, 485, 899
AluI AGCT 6 cut(s) 14, 344, 354, 479, 485, 899
Alw21I GWGCWC 2 cut(s) 481, 658
Alw26I GTCTC 3 cut(s) 114, 827, 884
Alw44I GTGCAC 1 cut(s) 654
Ama87I CYCGRG 2 cut(s) 674, 914
AoxI GGCC 3 cut(s) 168, 772, 910
ApaLI GTGCAC 1 cut(s) 654
ApeKI GCWGC 3 cut(s) 485, 790, 899
ApoI RAATTY 4 cut(s) 178, 244, 662, 1081
Asp700I GAANNNNTTC 1 cut(s) 459
AspS9I GGNCC 3 cut(s) 333, 589, 683
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 1 cut(s) 34
AsuII TTCGAA 1 cut(s) 339
AvaI CYCGRG 2 cut(s) 674, 914
AvaII GGWCC 3 cut(s) 333, 589, 683
BaeGI GKGCMC 1 cut(s) 658
BaeI ACNNNNGTAYC 2 cut(s) 178, 211
BalI TGGCCA 1 cut(s) 170
BanI GGYRCC 1 cut(s) 386
BanII GRGCYC 1 cut(s) 481
Bbv12I GWGCWC 2 cut(s) 481, 658
BbvI GCAGC 3 cut(s) 472, 802, 886
BccI CCATC 7 cut(s) 53, 167, 504, 758, 764, 877, 1008
BceAI ACGGC 1 cut(s) 400
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 3 cut(s) 114, 827, 884
BfmI CTRYAG 2 cut(s) 249, 1005
BisI GCNGC 3 cut(s) 486, 791, 900
BlsI GCNGC 3 cut(s) 487, 792, 901
Bme1390I CCNGG 1 cut(s) 223
Bme18I GGWCC 3 cut(s) 333, 589, 683
BmeT110I CYCGRG 2 cut(s) 674, 914
BmgT120I GGNCC 3 cut(s) 333, 589, 683
BmiI GGNNCC 7 cut(s) 99, 334, 388, 685, 936, 977, 1051
BmrFI CCNGG 1 cut(s) 223
BmrI ACTGGG 1 cut(s) 818
BmsI GCATC 3 cut(s) 597, 777, 852
BmuI ACTGGG 1 cut(s) 818
BpmI CTGGAG 2 cut(s) 366, 384
Bpu14I TTCGAA 1 cut(s) 339
BpuEI CTTGAG 2 cut(s) 465, 761
BpuMI CCSGG 1 cut(s) 223
Bsa29I ATCGAT 1 cut(s) 198
BsaBI GATNNNNATC 1 cut(s) 399
BsaJI CCNNGG 3 cut(s) 6, 171, 913
BsaWI WCCGGW 1 cut(s) 586
Bsc4I CCNNNNNNNGG 5 cut(s) 191, 366, 598, 809, 972
Bse118I RCCGGY 1 cut(s) 802
Bse1I ACTGG 4 cut(s) 592, 685, 813, 947
Bse3DI GCAATG 3 cut(s) 241, 541, 739
Bse8I GATNNNNATC 1 cut(s) 399
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 3 cut(s) 6, 171, 913
BseGI GGATG 6 cut(s) 139, 560, 698, 718, 953, 1000
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 5 cut(s) 191, 366, 598, 809, 972
BseMI GCAATG 3 cut(s) 241, 541, 739
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 4 cut(s) 592, 685, 813, 947
BseSI GKGCMC 1 cut(s) 658
BseXI GCAGC 3 cut(s) 472, 802, 886
BshFI GGCC 3 cut(s) 170, 774, 912
BshNI GGYRCC 1 cut(s) 386
BshVI ATCGAT 1 cut(s) 198
BsiHKAI GWGCWC 2 cut(s) 481, 658
BsiHKCI CYCGRG 2 cut(s) 674, 914
BsiSI CCGG 3 cut(s) 222, 587, 803
BslFI GGGAC 2 cut(s) 691, 1049
BslI CCNNNNNNNGG 5 cut(s) 191, 366, 598, 809, 972
BsmAI GTCTC 3 cut(s) 114, 827, 884
BsmFI GGGAC 2 cut(s) 691, 1049
BsnI GGCC 3 cut(s) 170, 774, 912
BsoBI CYCGRG 2 cut(s) 674, 914
Bsp119I TTCGAA 1 cut(s) 339
Bsp1286I GDGCHC 2 cut(s) 481, 658
Bsp19I CCATGG 1 cut(s) 6
BspANI GGCC 3 cut(s) 170, 774, 912
BspCNI CTCAG 1 cut(s) 103
BspDI ATCGAT 1 cut(s) 198
BspLI GGNNCC 7 cut(s) 99, 334, 388, 685, 936, 977, 1051
BspMAI CTGCAG 1 cut(s) 253
BspT104I TTCGAA 1 cut(s) 339
BspT107I GGYRCC 1 cut(s) 386
BsrDI GCAATG 3 cut(s) 241, 541, 739
BsrFI RCCGGY 1 cut(s) 802
BsrI ACTGG 4 cut(s) 592, 685, 813, 947
BssAI RCCGGY 1 cut(s) 802
BssECI CCNNGG 3 cut(s) 6, 171, 913
BssNAI GTATAC 1 cut(s) 944
BssT1I CCWWGG 2 cut(s) 6, 171
Bst1107I GTATAC 1 cut(s) 944
Bst4CI ACNGT 4 cut(s) 97, 194, 647, 1063
BstBI TTCGAA 1 cut(s) 339
BstC8I GCNNGC 2 cut(s) 16, 904
BstDEI CTNAG 2 cut(s) 90, 553
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 6 cut(s) 139, 560, 698, 718, 953, 1000
BstMAI GTCTC 3 cut(s) 114, 827, 884
BstMWI GCNNNNNNNGC 1 cut(s) 485
BstNSI RCATGY 2 cut(s) 518, 906
BstSCI CCNGG 1 cut(s) 221
BstSFI CTRYAG 2 cut(s) 249, 1005
BstSLI GKGCMC 1 cut(s) 658
BstV1I GCAGC 3 cut(s) 472, 802, 886
BstXI CCANNNNNNTGG 1 cut(s) 166
BstZ17I GTATAC 1 cut(s) 944
Bsu15I ATCGAT 1 cut(s) 198
BsuRI GGCC 3 cut(s) 170, 774, 912
BsuTUI ATCGAT 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 6
BtgZI GCGATG 1 cut(s) 58
BtsCI GGATG 6 cut(s) 139, 560, 698, 718, 953, 1000
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 2 cut(s) 25, 806
Cac8I GCNNGC 2 cut(s) 16, 904
Cfr10I RCCGGY 1 cut(s) 802
Cfr13I GGNCC 3 cut(s) 333, 589, 683
ClaI ATCGAT 1 cut(s) 198
CviAII CATG 3 cut(s) 7, 515, 903
DdeI CTNAG 2 cut(s) 90, 553
EaeI YGGCCR 1 cut(s) 168
Ecl136II GAGCTC 1 cut(s) 479
Eco130I CCWWGG 2 cut(s) 6, 171
Eco24I GRGCYC 1 cut(s) 481
Eco47I GGWCC 3 cut(s) 333, 589, 683
Eco53kI GAGCTC 1 cut(s) 479
Eco88I CYCGRG 2 cut(s) 674, 914
EcoICRI GAGCTC 1 cut(s) 479
EcoO109I RGGNCCY 1 cut(s) 333
EcoRI GAATTC 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 6, 171
EcoT38I GRGCYC 1 cut(s) 481
ErhI CCWWGG 2 cut(s) 6, 171
FaeI CATG 3 cut(s) 10, 518, 906
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FaqI GGGAC 2 cut(s) 691, 1049
FatI CATG 3 cut(s) 6, 514, 902
FauNDI CATATG 1 cut(s) 981
FblI GTMKAC 2 cut(s) 614, 943
Fnu4HI GCNGC 3 cut(s) 486, 791, 900
FokI GGATG 6 cut(s) 146, 547, 705, 725, 940, 987
FriOI GRGCYC 1 cut(s) 481
Fsp4HI GCNGC 3 cut(s) 486, 791, 900
GluI GCNGC 3 cut(s) 486, 791, 900
GsuI CTGGAG 2 cut(s) 366, 384
HaeIII GGCC 3 cut(s) 170, 774, 912
HapII CCGG 3 cut(s) 222, 587, 803
Hin1II CATG 3 cut(s) 10, 518, 906
HindIII AAGCTT 2 cut(s) 12, 352
HinfI GANTC 4 cut(s) 122, 375, 557, 865
HpaII CCGG 3 cut(s) 222, 587, 803
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 3 cut(s) 615, 656, 944
Hpy188I TCNGA 3 cut(s) 93, 207, 1097
Hpy188III TCNNGA 4 cut(s) 363, 676, 832, 862
Hpy8I GTNNAC 3 cut(s) 615, 656, 944
HpyAV CCTTC 4 cut(s) 346, 702, 835, 966
HpyCH4III ACNGT 4 cut(s) 97, 194, 647, 1063
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 8 cut(s) 18, 234, 251, 656, 732, 758, 790, 902
HpyF10VI GCNNNNNNNGC 1 cut(s) 485
HpyF3I CTNAG 2 cut(s) 90, 553
HpySE526I ACGT 1 cut(s) 540
Hsp92II CATG 3 cut(s) 10, 518, 906
LmnI GCTCC 3 cut(s) 476, 940, 968
Lsp1109I GCAGC 3 cut(s) 472, 802, 886
LweI GCATC 3 cut(s) 597, 777, 852
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 3 cut(s) 140, 408, 851
MboII GAAGA 7 cut(s) 31, 40, 370, 467, 820, 838, 1090
MfeI CAATTG 1 cut(s) 794
MhlI GDGCHC 2 cut(s) 481, 658
MlsI TGGCCA 1 cut(s) 170
MluCI AATT 8 cut(s) 148, 178, 244, 470, 662, 669, 794, 1081
MluNI TGGCCA 1 cut(s) 170
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 2 cut(s) 230, 670
MnlI CCTC 8 cut(s) 195, 219, 359, 370, 785, 923, 1003, 1039
Mox20I TGGCCA 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 459
MscI TGGCCA 1 cut(s) 170
MseI TTAA 4 cut(s) 621, 632, 885, 924
MslI CAYNNNNRTG 2 cut(s) 164, 448
Msp20I TGGCCA 1 cut(s) 170
MspI CCGG 3 cut(s) 222, 587, 803
MspR9I CCNGG 1 cut(s) 223
MunI CAATTG 1 cut(s) 794
MwoI GCNNNNNNNGC 1 cut(s) 485
NciI CCSGG 1 cut(s) 223
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 981
NlaIII CATG 3 cut(s) 10, 518, 906
NlaIV GGNNCC 7 cut(s) 99, 334, 388, 685, 936, 977, 1051
NmuCI GTSAC 1 cut(s) 408
NspI RCATGY 2 cut(s) 518, 906
NspV TTCGAA 1 cut(s) 339
OliI CACNNNNGTG 1 cut(s) 448
PaeI GCATGC 1 cut(s) 906
PciI ACATGT 1 cut(s) 514
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 3 cut(s) 375, 557, 865
PflMI CCANNNNNTGG 1 cut(s) 598
PkrI GCNGC 3 cut(s) 487, 792, 901
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
PpuMI RGGWCCY 1 cut(s) 333
PscI ACATGT 1 cut(s) 514
Psp124BI GAGCTC 1 cut(s) 481
Psp5II RGGWCCY 1 cut(s) 333
PspN4I GGNNCC 7 cut(s) 99, 334, 388, 685, 936, 977, 1051
PspPI GGNCC 3 cut(s) 333, 589, 683
PspPPI RGGWCCY 1 cut(s) 333
PstI CTGCAG 1 cut(s) 253
RseI CAYNNNNRTG 2 cut(s) 164, 448
SacI GAGCTC 1 cut(s) 481
SaqAI TTAA 4 cut(s) 621, 632, 885, 924
SatI GCNGC 3 cut(s) 486, 791, 900
Sau96I GGNCC 3 cut(s) 333, 589, 683
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 1 cut(s) 223
SduI GDGCHC 2 cut(s) 481, 658
SfaNI GCATC 3 cut(s) 597, 777, 852
SfcI CTRYAG 2 cut(s) 249, 1005
SfuI TTCGAA 1 cut(s) 339
SinI GGWCC 3 cut(s) 333, 589, 683
SmiMI CAYNNNNRTG 2 cut(s) 164, 448
SmlI CTYRAG 2 cut(s) 480, 776
SmoI CTYRAG 2 cut(s) 480, 776
SphI GCATGC 1 cut(s) 906
Sse9I AATT 8 cut(s) 148, 178, 244, 470, 662, 669, 794, 1081
SstI GAGCTC 1 cut(s) 481
StyD4I CCNGG 1 cut(s) 221
StyI CCWWGG 2 cut(s) 6, 171
TaaI ACNGT 4 cut(s) 97, 194, 647, 1063
TaiI ACGT 1 cut(s) 543
TaqI TCGA 4 cut(s) 198, 339, 503, 831
TasI AATT 8 cut(s) 148, 178, 244, 470, 662, 669, 794, 1081
TfiI GAWTC 3 cut(s) 375, 557, 865
Tru1I TTAA 4 cut(s) 621, 632, 885, 924
Tru9I TTAA 4 cut(s) 621, 632, 885, 924
TscAI CASTG 2 cut(s) 25, 813
TseFI GTSAC 1 cut(s) 408
TseI GCWGC 3 cut(s) 485, 790, 899
Tsp45I GTSAC 1 cut(s) 408
TspDTI ATGAA 6 cut(s) 61, 411, 549, 750, 942, 1085
TspGWI ACGGA 2 cut(s) 839, 894
TspRI CASTG 2 cut(s) 25, 813
Van91I CCANNNNNTGG 1 cut(s) 598
VneI GTGCAC 1 cut(s) 654
VpaK11BI GGWCC 3 cut(s) 333, 589, 683
XapI RAATTY 4 cut(s) 178, 244, 662, 1081
XceI RCATGY 2 cut(s) 518, 906
XmiI GTMKAC 2 cut(s) 614, 943
XmnI GAANNNNTTC 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.