FvH4_3g19750

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002678
Physical Location & Seq
Forward (+)
12919733 .. 12921885
2153 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g19750.t2

Sequence Viewer

Length: 1098 bp
ATGGCCATGGAAGCTTGCAGTGTTTCTCCTACGACCTTCTTCACCATCTTCTCCATTATCCTTTCTGTGTTCATCGCTTTCTCAGATGCTCAGTCGACGGTTCCGGCTATTTACATCTTCGGAGACTCCTTATTGGATGTTGGTAACAACAATTACCTTACCGTCTCTGTCGCCAAGGCAAATTTTCCTCACAACGGTATCGATTTTCCGACCAAGAAAGCAACCGGGAGGTTTTCCAATGGCAAGAATTCTGCAGATTTCTTGGCTGAGAAAGTAGGGTTGCCAACTTCCCCACCATACCTGTCACTTGTATCAAAATCCAATAAGCAAAAGGGGTCCTTCGTGGCCGGAGTTAGCTTCGCCTCTGGAGGGGCTGGAATCTTCAACGGCACCGATGATGAATATCGTCAGTCACTACCTTTGACAAAACAAGTAGGCTACTATGCACAAGTGTGTGCAGAGCTTCAGCAACAATTAGGAACTCAAGCTGCCCAAGCTCATCTAGCGAAATCTCTACATGTTGTTCTTATTGGAAGCAACGACGTTTTCGGCTACTTAGATTCATCTAACACCCAAAAGCAATATACACCGGACCAGTATGTCAGTTTGATGCTGTCTACCCTTAAACAACACTTGCAGAGAATACATAGTCTTGGTGCTCGGAAATTCGTTATTCTCGGCACTGGACCAGTTGGATGTTGTCCGGCAAGAAGGAGTAAAACAGGAGACTGCAATGAGTTAGGAAACTCCATCTCGTTAAAGTATAGTGATGGCCTCAAGTCAATGATGCAGCAATTGAAACCAGCATTGGGCATCAACTACTCCTTCTTCGAGACTTATAGCATTTTCCGTAACATCGTCCAAACTCCTTCTTCTTACGGATTCAATGAGACTAAAGCTGCATGCTGTGGCCTCGGCTATCTTAACGCAAAGGTTCCTTGTCTACCAGTCGCATCCTACTGCTCCAATAGAAGGGATCATATGTTCTGGGATCTTTTCCATCCTACAGAGGCAACTCACAAAATCTTAGTGGAACATCTTTTTGAGGGTTCCGCATACTCATTTCCAATGAATGTGAAGAAGTTAGTTTCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

366

Amino Acids

39.79

Weight (kDa)

8.83

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 270 1.7e-21 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 599
AccB1I GGYRCC 1 cut(s) 389
AccI GTMKAC 3 cut(s) 95, 617, 943
AciI CCGC 1 cut(s) 1053
AclWI GGATC 2 cut(s) 984, 999
AcoI YGGCCR 2 cut(s) 3, 345
AcsI RAATTY 3 cut(s) 181, 247, 665
AcuI CTGAAG 1 cut(s) 449
AfiI CCNNNNNNNGG 4 cut(s) 194, 369, 809, 972
AflIII ACRYGT 1 cut(s) 517
AgsI TTSAA 3 cut(s) 385, 799, 886
AjuI GAANNNNNNNTTGG 2 cut(s) 791, 823
AleI CACNNNNGTG 1 cut(s) 451
AloI GAACNNNNNNTCC 2 cut(s) 968, 1000
AluBI AGCT 6 cut(s) 14, 357, 463, 488, 497, 899
AluI AGCT 6 cut(s) 14, 357, 463, 488, 497, 899
Alw21I GWGCWC 1 cut(s) 661
Alw26I GTCTC 5 cut(s) 117, 169, 720, 827, 884
AlwI GGATC 2 cut(s) 984, 999
AoxI GGCC 4 cut(s) 3, 345, 772, 910
ApeKI GCWGC 3 cut(s) 488, 790, 899
ApoI RAATTY 3 cut(s) 181, 247, 665
AspS9I GGNCC 3 cut(s) 336, 592, 686
AsuC2I CCSGG 1 cut(s) 226
AsuHPI GGTGA 1 cut(s) 34
AvaII GGWCC 3 cut(s) 336, 592, 686
BaeI ACNNNNGTAYC 2 cut(s) 181, 214
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 661
BbvI GCAGC 3 cut(s) 475, 802, 886
BccI CCATC 4 cut(s) 53, 758, 764, 1008
BceAI ACGGC 1 cut(s) 403
BcnI CCSGG 1 cut(s) 226
BcoDI GTCTC 5 cut(s) 117, 169, 720, 827, 884
BfaI CTAG 1 cut(s) 503
BfmI CTRYAG 2 cut(s) 252, 1005
BisI GCNGC 3 cut(s) 489, 791, 900
BlsI GCNGC 3 cut(s) 490, 792, 901
Bme1390I CCNGG 1 cut(s) 226
Bme18I GGWCC 3 cut(s) 336, 592, 686
BmgT120I GGNCC 3 cut(s) 336, 592, 686
BmiI GGNNCC 5 cut(s) 102, 337, 391, 936, 1051
BmrFI CCNGG 1 cut(s) 226
BmsI GCATC 5 cut(s) 76, 600, 777, 822, 962
BpmI CTGGAG 1 cut(s) 387
BpuEI CTTGAG 2 cut(s) 468, 761
BpuMI CCSGG 1 cut(s) 226
Bsa29I ATCGAT 1 cut(s) 201
BsaBI GATNNNNATC 1 cut(s) 402
BsaJI CCNNGG 3 cut(s) 6, 174, 913
BsaWI WCCGGW 1 cut(s) 589
Bsc4I CCNNNNNNNGG 4 cut(s) 194, 369, 809, 972
Bse1I ACTGG 4 cut(s) 595, 688, 689, 947
Bse3DI GCAATG 1 cut(s) 739
Bse8I GATNNNNATC 1 cut(s) 402
BseCI ATCGAT 1 cut(s) 201
BseDI CCNNGG 3 cut(s) 6, 174, 913
BseGI GGATG 4 cut(s) 142, 701, 953, 1000
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 4 cut(s) 194, 369, 809, 972
BseMI GCAATG 1 cut(s) 739
BseMII CTCAG 3 cut(s) 96, 104, 258
BseNI ACTGG 4 cut(s) 595, 688, 689, 947
BseXI GCAGC 3 cut(s) 475, 802, 886
BsgI GTGCAG 1 cut(s) 477
BshFI GGCC 4 cut(s) 5, 347, 774, 912
BshNI GGYRCC 1 cut(s) 389
BshVI ATCGAT 1 cut(s) 201
BsiHKAI GWGCWC 1 cut(s) 661
BsiSI CCGG 5 cut(s) 104, 225, 348, 590, 704
BslI CCNNNNNNNGG 4 cut(s) 194, 369, 809, 972
BsmAI GTCTC 5 cut(s) 117, 169, 720, 827, 884
BsmBI CGTCTC 1 cut(s) 169
BsnI GGCC 4 cut(s) 5, 347, 774, 912
Bsp1286I GDGCHC 1 cut(s) 661
Bsp143I GATC 2 cut(s) 976, 991
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 1 cut(s) 1053
BspANI GGCC 4 cut(s) 5, 347, 774, 912
BspCNI CTCAG 3 cut(s) 95, 103, 259
BspDI ATCGAT 1 cut(s) 201
BspLI GGNNCC 5 cut(s) 102, 337, 391, 936, 1051
BspMAI CTGCAG 1 cut(s) 256
BspPI GGATC 2 cut(s) 984, 999
BspT107I GGYRCC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 739
BsrI ACTGG 4 cut(s) 595, 688, 689, 947
BssECI CCNNGG 3 cut(s) 6, 174, 913
BssMI GATC 2 cut(s) 976, 991
BssT1I CCWWGG 2 cut(s) 6, 174
Bst4CI ACNGT 3 cut(s) 100, 163, 197
BstC8I GCNNGC 2 cut(s) 16, 904
BstDEI CTNAG 5 cut(s) 82, 90, 267, 556, 1027
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 4 cut(s) 142, 701, 953, 1000
BstKTI GATC 2 cut(s) 979, 994
BstMAI GTCTC 5 cut(s) 117, 169, 720, 827, 884
BstMBI GATC 2 cut(s) 976, 991
BstMWI GCNNNNNNNGC 3 cut(s) 11, 494, 503
BstNSI RCATGY 2 cut(s) 521, 906
BstSCI CCNGG 1 cut(s) 224
BstSFI CTRYAG 2 cut(s) 252, 1005
BstV1I GCAGC 3 cut(s) 475, 802, 886
BstX2I RGATCY 1 cut(s) 991
BstYI RGATCY 1 cut(s) 991
Bsu15I ATCGAT 1 cut(s) 201
BsuRI GGCC 4 cut(s) 5, 347, 774, 912
BsuTUI ATCGAT 1 cut(s) 201
BtgI CCRYGG 1 cut(s) 6
BtgZI GCGATG 1 cut(s) 58
BtsCI GGATG 4 cut(s) 142, 701, 953, 1000
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 2 cut(s) 25, 681
Cac8I GCNNGC 2 cut(s) 16, 904
Cfr13I GGNCC 3 cut(s) 336, 592, 686
ClaI ATCGAT 1 cut(s) 201
CviAII CATG 3 cut(s) 7, 518, 903
DdeI CTNAG 5 cut(s) 82, 90, 267, 556, 1027
DpnI GATC 2 cut(s) 978, 993
DpnII GATC 2 cut(s) 976, 991
DrdI GACNNNNNNGTC 1 cut(s) 599
DseDI GACNNNNNNGTC 1 cut(s) 599
EaeI YGGCCR 2 cut(s) 3, 345
Eco130I CCWWGG 2 cut(s) 6, 174
Eco47I GGWCC 3 cut(s) 336, 592, 686
Eco57I CTGAAG 1 cut(s) 449
EcoO109I RGGNCCY 1 cut(s) 336
EcoRI GAATTC 1 cut(s) 247
EcoT14I CCWWGG 2 cut(s) 6, 174
ErhI CCWWGG 2 cut(s) 6, 174
Esp3I CGTCTC 1 cut(s) 169
FaeI CATG 3 cut(s) 10, 521, 906
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FatI CATG 3 cut(s) 6, 517, 902
FauNDI CATATG 1 cut(s) 981
FblI GTMKAC 3 cut(s) 95, 617, 943
Fnu4HI GCNGC 3 cut(s) 489, 791, 900
FokI GGATG 4 cut(s) 149, 708, 940, 987
Fsp4HI GCNGC 3 cut(s) 489, 791, 900
FspBI CTAG 1 cut(s) 503
GluI GCNGC 3 cut(s) 489, 791, 900
GsuI CTGGAG 1 cut(s) 387
HaeIII GGCC 4 cut(s) 5, 347, 774, 912
HapII CCGG 5 cut(s) 104, 225, 348, 590, 704
Hin1II CATG 3 cut(s) 10, 521, 906
HincII GTYRAC 1 cut(s) 96
HindII GTYRAC 1 cut(s) 96
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 4 cut(s) 125, 378, 560, 882
HpaII CCGG 5 cut(s) 104, 225, 348, 590, 704
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 3 cut(s) 96, 618, 944
Hpy188I TCNGA 5 cut(s) 85, 122, 210, 663, 1097
Hpy188III TCNNGA 2 cut(s) 366, 832
Hpy8I GTNNAC 3 cut(s) 96, 618, 944
Hpy99I CGWCG 2 cut(s) 100, 545
HpyAV CCTTC 6 cut(s) 46, 349, 705, 835, 879, 966
HpyCH4III ACNGT 3 cut(s) 100, 163, 197
HpyCH4IV ACGT 1 cut(s) 543
HpyCH4V TGCA 8 cut(s) 18, 254, 446, 458, 637, 732, 790, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 494, 503
HpyF3I CTNAG 5 cut(s) 82, 90, 267, 556, 1027
HpySE526I ACGT 1 cut(s) 543
Hsp92II CATG 3 cut(s) 10, 521, 906
Kzo9I GATC 2 cut(s) 976, 991
LmnI GCTCC 1 cut(s) 968
Lsp1109I GCAGC 3 cut(s) 475, 802, 886
LweI GCATC 5 cut(s) 76, 600, 777, 822, 962
MaeI CTAG 1 cut(s) 503
MaeII ACGT 1 cut(s) 543
MaeIII GTNAC 4 cut(s) 143, 303, 411, 851
MalI GATC 2 cut(s) 978, 993
MboI GATC 2 cut(s) 976, 991
MboII GAAGA 7 cut(s) 31, 40, 109, 373, 820, 864, 1090
MfeI CAATTG 1 cut(s) 794
MflI RGATCY 1 cut(s) 991
MhlI GDGCHC 1 cut(s) 661
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 151, 181, 247, 473, 665, 794
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 2 cut(s) 233, 673
MnlI CCTC 8 cut(s) 198, 222, 362, 373, 785, 923, 1003, 1039
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 624, 758, 924
MslI CAYNNNNRTG 1 cut(s) 451
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 5 cut(s) 104, 225, 348, 590, 704
MspR9I CCNGG 1 cut(s) 226
MunI CAATTG 1 cut(s) 794
MwoI GCNNNNNNNGC 3 cut(s) 11, 494, 503
NciI CCSGG 1 cut(s) 226
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 981
NdeII GATC 2 cut(s) 976, 991
NlaIII CATG 3 cut(s) 10, 521, 906
NlaIV GGNNCC 5 cut(s) 102, 337, 391, 936, 1051
NmeAIII GCCGAG 2 cut(s) 657, 894
NmuCI GTSAC 2 cut(s) 303, 411
NspI RCATGY 2 cut(s) 521, 906
OliI CACNNNNGTG 1 cut(s) 451
PaeI GCATGC 1 cut(s) 906
PciI ACATGT 1 cut(s) 517
PfeI GAWTC 3 cut(s) 378, 560, 882
PkrI GCNGC 3 cut(s) 490, 792, 901
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
PpuMI RGGWCCY 1 cut(s) 336
PscI ACATGT 1 cut(s) 517
Psp5II RGGWCCY 1 cut(s) 336
PspN4I GGNNCC 5 cut(s) 102, 337, 391, 936, 1051
PspPI GGNCC 3 cut(s) 336, 592, 686
PspPPI RGGWCCY 1 cut(s) 336
PstI CTGCAG 1 cut(s) 256
PsuI RGATCY 1 cut(s) 991
RseI CAYNNNNRTG 1 cut(s) 451
SalI GTCGAC 1 cut(s) 94
SaqAI TTAA 3 cut(s) 624, 758, 924
SatI GCNGC 3 cut(s) 489, 791, 900
Sau3AI GATC 2 cut(s) 976, 991
Sau96I GGNCC 3 cut(s) 336, 592, 686
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 1 cut(s) 226
SduI GDGCHC 1 cut(s) 661
SfaNI GCATC 5 cut(s) 76, 600, 777, 822, 962
SfcI CTRYAG 2 cut(s) 252, 1005
SinI GGWCC 3 cut(s) 336, 592, 686
SmiMI CAYNNNNRTG 1 cut(s) 451
SmlI CTYRAG 2 cut(s) 483, 776
SmoI CTYRAG 2 cut(s) 483, 776
SphI GCATGC 1 cut(s) 906
Sse9I AATT 6 cut(s) 151, 181, 247, 473, 665, 794
SsiI CCGC 1 cut(s) 1053
SspMI CTAG 1 cut(s) 503
StyD4I CCNGG 1 cut(s) 224
StyI CCWWGG 2 cut(s) 6, 174
TaaI ACNGT 3 cut(s) 100, 163, 197
TaiI ACGT 1 cut(s) 546
TaqI TCGA 3 cut(s) 95, 201, 831
TasI AATT 6 cut(s) 151, 181, 247, 473, 665, 794
TfiI GAWTC 3 cut(s) 378, 560, 882
Tru1I TTAA 3 cut(s) 624, 758, 924
Tru9I TTAA 3 cut(s) 624, 758, 924
TscAI CASTG 2 cut(s) 25, 688
TseFI GTSAC 2 cut(s) 303, 411
TseI GCWGC 3 cut(s) 488, 790, 899
Tsp45I GTSAC 2 cut(s) 303, 411
TspDTI ATGAA 4 cut(s) 61, 414, 552, 1085
TspGWI ACGGA 2 cut(s) 839, 894
TspRI CASTG 2 cut(s) 25, 688
VpaK11BI GGWCC 3 cut(s) 336, 592, 686
XapI RAATTY 3 cut(s) 181, 247, 665
XceI RCATGY 2 cut(s) 521, 906
XmiI GTMKAC 3 cut(s) 95, 617, 943
XspI CTAG 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.