Rh5CG257600

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0002678
Physical Location & Seq
Forward (+)
27365013 .. 27367246
2234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG257600.1

Sequence Viewer

Length: 969 bp
ATGACCATGGAAGCTTGCAGTGTTTCTCCTACGATTTTCTTCACCATCTTCTCCATTATCGTTTCTGTGTTCATCGCTTTCTCAAATGCTCAGACGGTTCCGGCCATTTACATATTTGGAGACTCTCTATTGGATGTTGGTAACAACAATTACCTTACCATTTCTGTGGCCAAGGCAAATTTTCCTCACAACGGTATAGATTTTCCGACCAAGAAACCAAACGGGAGGTTTTGCAATGGCAAGAATTCTGCAGATTTTCTGGCTGAGAAAGTAGGATTGCCAACTTCCCCACCATATCTGTCGCTTGTATCAAAATCTAATAAGCAAAAGGGGTCCTTCGAAGCTGGAGTAAGCTTCGCCTCTGGAGGGGCTGGAATCTTCAACGGCACCGATGATGAATATCGTCAGTCACTACCTTTGACAAAACAAATAGGCTACTATTCACAAGTGTGTGAAGAACTTCGGCAACAATTAGGAGCTCAAGCTGCCCAAGACCATCTATCGAAATCTCTACATGTCGTTCTTATTGGAAGCAATGACGTTTTCGGCTACTTAGATTCATCCAGCACCCAAAAGCAATACACACCGGACCAGTATGTGGATTTGATGCTGTCTACTCTCAAACAACACTTAAAGAGAATACACAGTCTTGGTGCACGAAAATTTGTAATTCTCGGGACTGGACCCGTTGGATGTTGTCCAGCACGAAGGGATGATAAAACAGGAGAGTGCAATGAAGTAGGAAACTCCATCTCTGCAAAGTATAGTGATGGCCTCAAGTCAATGATGCAGCAATTGAAACCGGCACTGGGCATAAACTACTCCTTCTTCGAGACTTATGGCATCTTCCGTAACATCGTCCAGAATCCATCAACTTACGGTACTCATATTCTCCTTATAAGCAGAATGTTCTATTTCCATATAGTCTATCATGTTATACTACCAGACTGTTCGTTTGGATATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

322

Amino Acids

35.4

Weight (kDa)

8.36

Isoelectric Point (pI)

32.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 281 5.8e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
AccB1I GGYRCC 1 cut(s) 386
AccB7I CCANNNNNTGG 1 cut(s) 598
AccI GTMKAC 1 cut(s) 614
AcoI YGGCCR 2 cut(s) 102, 168
AcsI RAATTY 3 cut(s) 178, 244, 662
AfaI GTAC 1 cut(s) 883
AfiI CCNNNNNNNGG 4 cut(s) 191, 366, 598, 809
AflIII ACRYGT 1 cut(s) 514
AgsI TTSAA 2 cut(s) 382, 799
AleI CACNNNNGTG 1 cut(s) 448
AluBI AGCT 5 cut(s) 14, 344, 354, 479, 485
AluI AGCT 5 cut(s) 14, 344, 354, 479, 485
Alw21I GWGCWC 2 cut(s) 481, 658
Alw26I GTCTC 2 cut(s) 114, 827
Alw44I GTGCAC 1 cut(s) 654
Ama87I CYCGRG 1 cut(s) 674
AoxI GGCC 3 cut(s) 102, 168, 772
ApaLI GTGCAC 1 cut(s) 654
ApeKI GCWGC 2 cut(s) 485, 790
ApoI RAATTY 3 cut(s) 178, 244, 662
ArsI GACNNNNNNTTYG 1 cut(s) 938
Asp700I GAANNNNTTC 1 cut(s) 459
AspS9I GGNCC 3 cut(s) 333, 589, 683
AsuHPI GGTGA 1 cut(s) 34
AsuII TTCGAA 1 cut(s) 339
AvaI CYCGRG 1 cut(s) 674
AvaII GGWCC 3 cut(s) 333, 589, 683
BaeGI GKGCMC 1 cut(s) 658
BalI TGGCCA 1 cut(s) 170
BanI GGYRCC 1 cut(s) 386
BanII GRGCYC 1 cut(s) 481
Bbv12I GWGCWC 2 cut(s) 481, 658
BbvI GCAGC 2 cut(s) 472, 802
BccI CCATC 5 cut(s) 53, 504, 758, 764, 877
BceAI ACGGC 1 cut(s) 400
BcoDI GTCTC 2 cut(s) 114, 827
BfmI CTRYAG 1 cut(s) 249
BisI GCNGC 2 cut(s) 486, 791
BlsI GCNGC 2 cut(s) 487, 792
Bme18I GGWCC 3 cut(s) 333, 589, 683
BmeT110I CYCGRG 1 cut(s) 674
BmgT120I GGNCC 3 cut(s) 333, 589, 683
BmiI GGNNCC 4 cut(s) 99, 334, 388, 685
BmrI ACTGGG 1 cut(s) 818
BmsI GCATC 3 cut(s) 597, 777, 852
BmuI ACTGGG 1 cut(s) 818
BpmI CTGGAG 2 cut(s) 366, 384
Bpu14I TTCGAA 1 cut(s) 339
BpuEI CTTGAG 2 cut(s) 465, 761
BsaBI GATNNNNATC 1 cut(s) 399
BsaJI CCNNGG 2 cut(s) 6, 171
BsaWI WCCGGW 1 cut(s) 586
Bsc4I CCNNNNNNNGG 4 cut(s) 191, 366, 598, 809
Bse118I RCCGGY 1 cut(s) 802
Bse1I ACTGG 3 cut(s) 592, 685, 813
Bse3DI GCAATG 3 cut(s) 241, 541, 739
Bse8I GATNNNNATC 1 cut(s) 399
BseDI CCNNGG 2 cut(s) 6, 171
BseGI GGATG 4 cut(s) 139, 560, 698, 718
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 4 cut(s) 191, 366, 598, 809
BseMI GCAATG 3 cut(s) 241, 541, 739
BseMII CTCAG 2 cut(s) 104, 255
BseNI ACTGG 3 cut(s) 592, 685, 813
BseSI GKGCMC 1 cut(s) 658
BseXI GCAGC 2 cut(s) 472, 802
BshFI GGCC 3 cut(s) 104, 170, 774
BshNI GGYRCC 1 cut(s) 386
BsiHKAI GWGCWC 2 cut(s) 481, 658
BsiHKCI CYCGRG 1 cut(s) 674
BsiSI CCGG 3 cut(s) 101, 587, 803
BslFI GGGAC 1 cut(s) 691
BslI CCNNNNNNNGG 4 cut(s) 191, 366, 598, 809
BsmAI GTCTC 2 cut(s) 114, 827
BsmFI GGGAC 1 cut(s) 691
BsnI GGCC 3 cut(s) 104, 170, 774
BsoBI CYCGRG 1 cut(s) 674
Bsp119I TTCGAA 1 cut(s) 339
Bsp1286I GDGCHC 2 cut(s) 481, 658
Bsp19I CCATGG 1 cut(s) 6
BspANI GGCC 3 cut(s) 104, 170, 774
BspCNI CTCAG 2 cut(s) 103, 256
BspHI TCATGA 1 cut(s) 965
BspLI GGNNCC 4 cut(s) 99, 334, 388, 685
BspMAI CTGCAG 1 cut(s) 253
BspT104I TTCGAA 1 cut(s) 339
BspT107I GGYRCC 1 cut(s) 386
BsrDI GCAATG 3 cut(s) 241, 541, 739
BsrFI RCCGGY 1 cut(s) 802
BsrI ACTGG 3 cut(s) 592, 685, 813
BssAI RCCGGY 1 cut(s) 802
BssECI CCNNGG 2 cut(s) 6, 171
BssT1I CCWWGG 2 cut(s) 6, 171
Bst4CI ACNGT 5 cut(s) 97, 194, 647, 881, 950
BstBI TTCGAA 1 cut(s) 339
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 3 cut(s) 90, 264, 553
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 4 cut(s) 139, 560, 698, 718
BstMAI GTCTC 2 cut(s) 114, 827
BstMWI GCNNNNNNNGC 1 cut(s) 485
BstNSI RCATGY 1 cut(s) 518
BstSFI CTRYAG 1 cut(s) 249
BstSLI GKGCMC 1 cut(s) 658
BstV1I GCAGC 2 cut(s) 472, 802
BstXI CCANNNNNNTGG 1 cut(s) 166
BsuRI GGCC 3 cut(s) 104, 170, 774
BtgI CCRYGG 1 cut(s) 6
BtgZI GCGATG 1 cut(s) 58
BtsCI GGATG 4 cut(s) 139, 560, 698, 718
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 2 cut(s) 25, 806
Cac8I GCNNGC 1 cut(s) 16
CciI TCATGA 1 cut(s) 965
Cfr10I RCCGGY 1 cut(s) 802
Cfr13I GGNCC 3 cut(s) 333, 589, 683
Csp6I GTAC 1 cut(s) 882
CviAII CATG 4 cut(s) 7, 515, 932, 966
CviQI GTAC 1 cut(s) 882
DdeI CTNAG 3 cut(s) 90, 264, 553
EaeI YGGCCR 2 cut(s) 102, 168
Ecl136II GAGCTC 1 cut(s) 479
Eco130I CCWWGG 2 cut(s) 6, 171
Eco24I GRGCYC 1 cut(s) 481
Eco47I GGWCC 3 cut(s) 333, 589, 683
Eco53kI GAGCTC 1 cut(s) 479
Eco88I CYCGRG 1 cut(s) 674
EcoICRI GAGCTC 1 cut(s) 479
EcoO109I RGGNCCY 1 cut(s) 333
EcoRI GAATTC 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 6, 171
EcoT38I GRGCYC 1 cut(s) 481
ErhI CCWWGG 2 cut(s) 6, 171
FaeI CATG 4 cut(s) 10, 518, 935, 969
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FaqI GGGAC 1 cut(s) 691
FatI CATG 4 cut(s) 6, 514, 931, 965
FblI GTMKAC 1 cut(s) 614
Fnu4HI GCNGC 2 cut(s) 486, 791
FokI GGATG 4 cut(s) 146, 547, 705, 725
FriOI GRGCYC 1 cut(s) 481
Fsp4HI GCNGC 2 cut(s) 486, 791
GluI GCNGC 2 cut(s) 486, 791
GsuI CTGGAG 2 cut(s) 366, 384
HaeIII GGCC 3 cut(s) 104, 170, 774
HapII CCGG 3 cut(s) 101, 587, 803
Hin1II CATG 4 cut(s) 10, 518, 935, 969
HindIII AAGCTT 2 cut(s) 12, 352
HinfI GANTC 4 cut(s) 122, 375, 557, 865
HpaII CCGG 3 cut(s) 101, 587, 803
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 2 cut(s) 615, 656
Hpy188I TCNGA 2 cut(s) 93, 207
Hpy188III TCNNGA 5 cut(s) 363, 676, 832, 862, 966
Hpy8I GTNNAC 2 cut(s) 615, 656
HpyAV CCTTC 3 cut(s) 346, 702, 835
HpyCH4III ACNGT 5 cut(s) 97, 194, 647, 881, 950
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 7 cut(s) 18, 234, 251, 656, 732, 758, 790
HpyF10VI GCNNNNNNNGC 1 cut(s) 485
HpyF3I CTNAG 3 cut(s) 90, 264, 553
HpySE526I ACGT 1 cut(s) 540
Hsp92II CATG 4 cut(s) 10, 518, 935, 969
LmnI GCTCC 1 cut(s) 476
Lsp1109I GCAGC 2 cut(s) 472, 802
LweI GCATC 3 cut(s) 597, 777, 852
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 3 cut(s) 140, 408, 851
MboII GAAGA 6 cut(s) 31, 40, 370, 467, 820, 838
MfeI CAATTG 1 cut(s) 794
MhlI GDGCHC 2 cut(s) 481, 658
MlsI TGGCCA 1 cut(s) 170
MluCI AATT 7 cut(s) 148, 178, 244, 470, 662, 669, 794
MluNI TGGCCA 1 cut(s) 170
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 2 cut(s) 230, 670
MnlI CCTC 5 cut(s) 195, 219, 359, 370, 785
Mox20I TGGCCA 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 459
MscI TGGCCA 1 cut(s) 170
MseI TTAA 1 cut(s) 632
MslI CAYNNNNRTG 2 cut(s) 164, 448
Msp20I TGGCCA 1 cut(s) 170
MspI CCGG 3 cut(s) 101, 587, 803
MunI CAATTG 1 cut(s) 794
MwoI GCNNNNNNNGC 1 cut(s) 485
NcoI CCATGG 1 cut(s) 6
NlaIII CATG 4 cut(s) 10, 518, 935, 969
NlaIV GGNNCC 4 cut(s) 99, 334, 388, 685
NmuCI GTSAC 1 cut(s) 408
NspI RCATGY 1 cut(s) 518
NspV TTCGAA 1 cut(s) 339
OliI CACNNNNGTG 1 cut(s) 448
PagI TCATGA 1 cut(s) 965
PciI ACATGT 1 cut(s) 514
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 3 cut(s) 375, 557, 865
PflMI CCANNNNNTGG 1 cut(s) 598
PkrI GCNGC 2 cut(s) 487, 792
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
PpuMI RGGWCCY 1 cut(s) 333
PscI ACATGT 1 cut(s) 514
PsiI TTATAA 1 cut(s) 899
Psp124BI GAGCTC 1 cut(s) 481
Psp5II RGGWCCY 1 cut(s) 333
PspN4I GGNNCC 4 cut(s) 99, 334, 388, 685
PspPI GGNCC 3 cut(s) 333, 589, 683
PspPPI RGGWCCY 1 cut(s) 333
PstI CTGCAG 1 cut(s) 253
RsaI GTAC 1 cut(s) 883
RsaNI GTAC 1 cut(s) 882
RseI CAYNNNNRTG 2 cut(s) 164, 448
SacI GAGCTC 1 cut(s) 481
SaqAI TTAA 1 cut(s) 632
SatI GCNGC 2 cut(s) 486, 791
Sau96I GGNCC 3 cut(s) 333, 589, 683
SchI GAGTC 1 cut(s) 116
SduI GDGCHC 2 cut(s) 481, 658
SetI ASST 9 cut(s) 16, 156, 230, 346, 356, 418, 481, 487, 543
SfaNI GCATC 3 cut(s) 597, 777, 852
SfcI CTRYAG 1 cut(s) 249
SfuI TTCGAA 1 cut(s) 339
SinI GGWCC 3 cut(s) 333, 589, 683
SmiMI CAYNNNNRTG 2 cut(s) 164, 448
SmlI CTYRAG 2 cut(s) 480, 776
SmoI CTYRAG 2 cut(s) 480, 776
Sse9I AATT 7 cut(s) 148, 178, 244, 470, 662, 669, 794
SstI GAGCTC 1 cut(s) 481
StyI CCWWGG 2 cut(s) 6, 171
TaaI ACNGT 5 cut(s) 97, 194, 647, 881, 950
TaiI ACGT 1 cut(s) 543
TaqI TCGA 3 cut(s) 339, 503, 831
TasI AATT 7 cut(s) 148, 178, 244, 470, 662, 669, 794
TfiI GAWTC 3 cut(s) 375, 557, 865
Tru1I TTAA 1 cut(s) 632
Tru9I TTAA 1 cut(s) 632
TscAI CASTG 2 cut(s) 25, 813
TseFI GTSAC 1 cut(s) 408
TseI GCWGC 2 cut(s) 485, 790
Tsp45I GTSAC 1 cut(s) 408
TspDTI ATGAA 5 cut(s) 61, 411, 549, 750, 954
TspGWI ACGGA 1 cut(s) 839
TspRI CASTG 2 cut(s) 25, 813
Van91I CCANNNNNTGG 1 cut(s) 598
VneI GTGCAC 1 cut(s) 654
VpaK11BI GGWCC 3 cut(s) 333, 589, 683
XapI RAATTY 3 cut(s) 178, 244, 662
XceI RCATGY 1 cut(s) 518
XmiI GTMKAC 1 cut(s) 614
XmnI GAANNNNTTC 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.