Rh5DG234600

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002678
Physical Location & Seq
Forward (+)
26779981 .. 26782209
2229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG234600.1

Sequence Viewer

Length: 885 bp
ATGCTCAGACGGTTCCGGCCATTTACATATTTGGACTCTCTATTGGATGTTGGTAACAACAATTACCTTACCATCTCTGTGGCCAAGGCAAATTTTCCTCACAACGGTATCGATTTTCCGACCAAGAAACCAACCGGGAGGTTTTGCAATGGCAAGAATTCTGCAGATTTTCTGGCTGAGAAAGTAGGATTGCCTACTTCCCCACCATATCTGTCGCTTTTATCAAAATCTAATAAGCAAAAGGGGTCCTTCGAAGCTGGAGTAAGCTTCGCCTCTGGAGGGGCTGGAATCTTCAACGGCACCGATGATGAATATCGTCAGTCACTACCTTTGACAAAACAAATAGACTACTATTCACAAGTTTGTGAAGAACTTCGGCAACAATTAGGAGCTCAAGCTGCCCAAGACCATCTATCGAAATCTCTACATGTCGTTCTTATTGGAAGCAATGACGTTTTCGGCTACTTAGATTCATCCAGCACCCAAAAGCAATACACACCGGATCAGTATGTGGATTTGATGCTGTCTACTCTTAAACAACACTTAAAGAGAATACACAGTCTTGGTGCACGAAAATTTGTAATTCTCGGGACTGGACCCGTTGGATGTTGTCCAGCACGAAGGGATGATAAAACAGGAGAGTGCAATGAAGTAGGAAACTCCATCTCTGCAAAGTATAGTGATGGTCTCAAGTCAATGATGCAGCAATTGAAACCGGCACTGGGCATAAACTACTCCTTCTTCGAGACTTATGGCATCTTCCGTAACATCGTCCAGAATCCATCAACTTACGGTACTCATATCTCCTTATTAGCAGAATGTTTTATTTCCATATATATAGTCTATCATGTTATACTACCAGACTGTTCGTTTGGATATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

294

Amino Acids

32.53

Weight (kDa)

8.56

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 12 - 254 3.1e-19 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 527
AclWI GGATC 1 cut(s) 510
AcoI YGGCCR 2 cut(s) 17, 81
AcsI RAATTY 3 cut(s) 91, 157, 575
AfaI GTAC 1 cut(s) 796
AfiI CCNNNNNNNGG 3 cut(s) 104, 279, 722
AflIII ACRYGT 1 cut(s) 427
AgsI TTSAA 2 cut(s) 295, 712
AluBI AGCT 4 cut(s) 257, 267, 392, 398
AluI AGCT 4 cut(s) 257, 267, 392, 398
Alw21I GWGCWC 2 cut(s) 394, 571
Alw26I GTCTC 2 cut(s) 692, 740
Alw44I GTGCAC 1 cut(s) 567
AlwI GGATC 1 cut(s) 510
Ama87I CYCGRG 1 cut(s) 587
AoxI GGCC 2 cut(s) 17, 81
ApaLI GTGCAC 1 cut(s) 567
ApeKI GCWGC 2 cut(s) 398, 703
ApoI RAATTY 3 cut(s) 91, 157, 575
ArsI GACNNNNNNTTYG 1 cut(s) 854
Asp700I GAANNNNTTC 1 cut(s) 372
AspS9I GGNCC 2 cut(s) 246, 596
AsuC2I CCSGG 1 cut(s) 136
AsuII TTCGAA 1 cut(s) 252
AvaI CYCGRG 1 cut(s) 587
AvaII GGWCC 2 cut(s) 246, 596
BaeGI GKGCMC 1 cut(s) 571
BaeI ACNNNNGTAYC 2 cut(s) 91, 124
BalI TGGCCA 1 cut(s) 83
BanI GGYRCC 1 cut(s) 299
BanII GRGCYC 1 cut(s) 394
Bbv12I GWGCWC 2 cut(s) 394, 571
BbvI GCAGC 2 cut(s) 385, 715
BccI CCATC 5 cut(s) 80, 417, 671, 677, 790
BceAI ACGGC 1 cut(s) 313
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 2 cut(s) 692, 740
BfmI CTRYAG 1 cut(s) 162
BisI GCNGC 2 cut(s) 399, 704
BlsI GCNGC 2 cut(s) 400, 705
Bme1390I CCNGG 1 cut(s) 136
Bme18I GGWCC 2 cut(s) 246, 596
BmeT110I CYCGRG 1 cut(s) 587
BmgT120I GGNCC 2 cut(s) 246, 596
BmiI GGNNCC 4 cut(s) 14, 247, 301, 598
BmrFI CCNGG 1 cut(s) 136
BmrI ACTGGG 1 cut(s) 731
BmsI GCATC 3 cut(s) 510, 690, 765
BmuI ACTGGG 1 cut(s) 731
BpmI CTGGAG 2 cut(s) 279, 297
Bpu14I TTCGAA 1 cut(s) 252
BpuEI CTTGAG 2 cut(s) 378, 674
BpuMI CCSGG 1 cut(s) 136
Bsa29I ATCGAT 1 cut(s) 111
BsaBI GATNNNNATC 1 cut(s) 312
BsaI GGTCTC 1 cut(s) 692
BsaJI CCNNGG 1 cut(s) 84
BsaWI WCCGGW 1 cut(s) 499
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 279, 722
Bse118I RCCGGY 1 cut(s) 715
Bse1I ACTGG 2 cut(s) 598, 726
Bse3DI GCAATG 3 cut(s) 154, 454, 652
Bse8I GATNNNNATC 1 cut(s) 312
BseCI ATCGAT 1 cut(s) 111
BseDI CCNNGG 1 cut(s) 84
BseGI GGATG 4 cut(s) 52, 473, 611, 631
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 3 cut(s) 104, 279, 722
BseMI GCAATG 3 cut(s) 154, 454, 652
BseMII CTCAG 2 cut(s) 19, 168
BseNI ACTGG 2 cut(s) 598, 726
BseSI GKGCMC 1 cut(s) 571
BseXI GCAGC 2 cut(s) 385, 715
BshFI GGCC 2 cut(s) 19, 83
BshNI GGYRCC 1 cut(s) 299
BshVI ATCGAT 1 cut(s) 111
BsiHKAI GWGCWC 2 cut(s) 394, 571
BsiHKCI CYCGRG 1 cut(s) 587
BsiSI CCGG 4 cut(s) 16, 135, 500, 716
BslFI GGGAC 1 cut(s) 604
BslI CCNNNNNNNGG 3 cut(s) 104, 279, 722
BsmAI GTCTC 2 cut(s) 692, 740
BsmFI GGGAC 1 cut(s) 604
BsnI GGCC 2 cut(s) 19, 83
Bso31I GGTCTC 1 cut(s) 692
BsoBI CYCGRG 1 cut(s) 587
Bsp119I TTCGAA 1 cut(s) 252
Bsp1286I GDGCHC 2 cut(s) 394, 571
Bsp143I GATC 1 cut(s) 502
BspANI GGCC 2 cut(s) 19, 83
BspCNI CTCAG 2 cut(s) 18, 169
BspDI ATCGAT 1 cut(s) 111
BspHI TCATGA 1 cut(s) 881
BspLI GGNNCC 4 cut(s) 14, 247, 301, 598
BspMAI CTGCAG 1 cut(s) 166
BspPI GGATC 1 cut(s) 510
BspT104I TTCGAA 1 cut(s) 252
BspT107I GGYRCC 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 692
BsrDI GCAATG 3 cut(s) 154, 454, 652
BsrFI RCCGGY 1 cut(s) 715
BsrI ACTGG 2 cut(s) 598, 726
BssAI RCCGGY 1 cut(s) 715
BssECI CCNNGG 1 cut(s) 84
BssMI GATC 1 cut(s) 502
BssT1I CCWWGG 1 cut(s) 84
Bst4CI ACNGT 5 cut(s) 12, 107, 560, 794, 866
BstBI TTCGAA 1 cut(s) 252
BstDEI CTNAG 3 cut(s) 5, 177, 466
BstF5I GGATG 4 cut(s) 52, 473, 611, 631
BstKTI GATC 1 cut(s) 505
BstMAI GTCTC 2 cut(s) 692, 740
BstMBI GATC 1 cut(s) 502
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstNSI RCATGY 1 cut(s) 431
BstSCI CCNGG 1 cut(s) 134
BstSFI CTRYAG 1 cut(s) 162
BstSLI GKGCMC 1 cut(s) 571
BstV1I GCAGC 2 cut(s) 385, 715
BstXI CCANNNNNNTGG 1 cut(s) 79
Bsu15I ATCGAT 1 cut(s) 111
BsuRI GGCC 2 cut(s) 19, 83
BsuTUI ATCGAT 1 cut(s) 111
BtsCI GGATG 4 cut(s) 52, 473, 611, 631
BtsIMutI CAGTG 1 cut(s) 719
CciI TCATGA 1 cut(s) 881
Cfr10I RCCGGY 1 cut(s) 715
Cfr13I GGNCC 2 cut(s) 246, 596
ClaI ATCGAT 1 cut(s) 111
Csp6I GTAC 1 cut(s) 795
CviAII CATG 3 cut(s) 428, 848, 882
CviJI RGCY 9 cut(s) 19, 83, 176, 257, 267, 284, 392, 398, 462
CviKI_1 RGCY 9 cut(s) 19, 83, 176, 257, 267, 284, 392, 398, 462
CviQI GTAC 1 cut(s) 795
DdeI CTNAG 3 cut(s) 5, 177, 466
DpnI GATC 1 cut(s) 504
DpnII GATC 1 cut(s) 502
EaeI YGGCCR 2 cut(s) 17, 81
Ecl136II GAGCTC 1 cut(s) 392
Eco130I CCWWGG 1 cut(s) 84
Eco24I GRGCYC 1 cut(s) 394
Eco31I GGTCTC 1 cut(s) 692
Eco47I GGWCC 2 cut(s) 246, 596
Eco53kI GAGCTC 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 587
EcoICRI GAGCTC 1 cut(s) 392
EcoO109I RGGNCCY 1 cut(s) 246
EcoRI GAATTC 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 84
EcoT38I GRGCYC 1 cut(s) 394
ErhI CCWWGG 1 cut(s) 84
FaeI CATG 3 cut(s) 431, 851, 885
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 1 cut(s) 604
FatI CATG 3 cut(s) 427, 847, 881
FblI GTMKAC 1 cut(s) 527
Fnu4HI GCNGC 2 cut(s) 399, 704
FokI GGATG 4 cut(s) 59, 460, 618, 638
FriOI GRGCYC 1 cut(s) 394
Fsp4HI GCNGC 2 cut(s) 399, 704
GluI GCNGC 2 cut(s) 399, 704
GsuI CTGGAG 2 cut(s) 279, 297
HaeIII GGCC 2 cut(s) 19, 83
HapII CCGG 4 cut(s) 16, 135, 500, 716
Hin1II CATG 3 cut(s) 431, 851, 885
HindIII AAGCTT 1 cut(s) 265
HinfI GANTC 4 cut(s) 35, 288, 470, 778
HpaII CCGG 4 cut(s) 16, 135, 500, 716
Hpy166II GTNNAC 2 cut(s) 528, 569
Hpy188I TCNGA 2 cut(s) 8, 120
Hpy188III TCNNGA 5 cut(s) 276, 589, 745, 775, 882
Hpy8I GTNNAC 2 cut(s) 528, 569
HpyAV CCTTC 3 cut(s) 259, 615, 748
HpyCH4III ACNGT 5 cut(s) 12, 107, 560, 794, 866
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 6 cut(s) 147, 164, 569, 645, 671, 703
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpyF3I CTNAG 3 cut(s) 5, 177, 466
HpySE526I ACGT 1 cut(s) 453
Hsp92II CATG 3 cut(s) 431, 851, 885
Kzo9I GATC 1 cut(s) 502
LmnI GCTCC 1 cut(s) 389
Lsp1109I GCAGC 2 cut(s) 385, 715
LweI GCATC 3 cut(s) 510, 690, 765
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 3 cut(s) 53, 321, 764
MalI GATC 1 cut(s) 504
MboI GATC 1 cut(s) 502
MboII GAAGA 4 cut(s) 283, 380, 733, 751
MfeI CAATTG 1 cut(s) 707
MhlI GDGCHC 2 cut(s) 394, 571
MlsI TGGCCA 1 cut(s) 83
MluCI AATT 7 cut(s) 61, 91, 157, 383, 575, 582, 707
MluNI TGGCCA 1 cut(s) 83
MlyI GAGTC 1 cut(s) 29
MmeI TCCRAC 2 cut(s) 143, 583
MnlI CCTC 4 cut(s) 108, 132, 272, 283
Mox20I TGGCCA 1 cut(s) 83
MroXI GAANNNNTTC 1 cut(s) 372
MscI TGGCCA 1 cut(s) 83
MseI TTAA 2 cut(s) 534, 545
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 1 cut(s) 83
MspI CCGG 4 cut(s) 16, 135, 500, 716
MspR9I CCNGG 1 cut(s) 136
MunI CAATTG 1 cut(s) 707
MwoI GCNNNNNNNGC 1 cut(s) 398
NciI CCSGG 1 cut(s) 136
NdeII GATC 1 cut(s) 502
NlaIII CATG 3 cut(s) 431, 851, 885
NlaIV GGNNCC 4 cut(s) 14, 247, 301, 598
NmuCI GTSAC 1 cut(s) 321
NspI RCATGY 1 cut(s) 431
NspV TTCGAA 1 cut(s) 252
PagI TCATGA 1 cut(s) 881
PciI ACATGT 1 cut(s) 427
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 3 cut(s) 288, 470, 778
PkrI GCNGC 2 cut(s) 400, 705
PleI GAGTC 1 cut(s) 29
PpsI GAGTC 1 cut(s) 29
PpuMI RGGWCCY 1 cut(s) 246
PscI ACATGT 1 cut(s) 427
Psp124BI GAGCTC 1 cut(s) 394
Psp5II RGGWCCY 1 cut(s) 246
PspN4I GGNNCC 4 cut(s) 14, 247, 301, 598
PspPI GGNCC 2 cut(s) 246, 596
PspPPI RGGWCCY 1 cut(s) 246
PstI CTGCAG 1 cut(s) 166
RsaI GTAC 1 cut(s) 796
RsaNI GTAC 1 cut(s) 795
RseI CAYNNNNRTG 1 cut(s) 77
SacI GAGCTC 1 cut(s) 394
SaqAI TTAA 2 cut(s) 534, 545
SatI GCNGC 2 cut(s) 399, 704
Sau3AI GATC 1 cut(s) 502
Sau96I GGNCC 2 cut(s) 246, 596
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 1 cut(s) 136
SduI GDGCHC 2 cut(s) 394, 571
SetI ASST 8 cut(s) 69, 143, 259, 269, 331, 394, 400, 456
SfaNI GCATC 3 cut(s) 510, 690, 765
SfcI CTRYAG 1 cut(s) 162
SfuI TTCGAA 1 cut(s) 252
SinI GGWCC 2 cut(s) 246, 596
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 2 cut(s) 393, 689
SmoI CTYRAG 2 cut(s) 393, 689
Sse9I AATT 7 cut(s) 61, 91, 157, 383, 575, 582, 707
SstI GAGCTC 1 cut(s) 394
StyD4I CCNGG 1 cut(s) 134
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 5 cut(s) 12, 107, 560, 794, 866
TaiI ACGT 1 cut(s) 456
TaqI TCGA 4 cut(s) 111, 252, 416, 744
TasI AATT 7 cut(s) 61, 91, 157, 383, 575, 582, 707
TfiI GAWTC 3 cut(s) 288, 470, 778
Tru1I TTAA 2 cut(s) 534, 545
Tru9I TTAA 2 cut(s) 534, 545
TscAI CASTG 1 cut(s) 726
TseFI GTSAC 1 cut(s) 321
TseI GCWGC 2 cut(s) 398, 703
Tsp45I GTSAC 1 cut(s) 321
TspDTI ATGAA 4 cut(s) 324, 462, 663, 870
TspGWI ACGGA 1 cut(s) 752
TspRI CASTG 1 cut(s) 726
VneI GTGCAC 1 cut(s) 567
VpaK11BI GGWCC 2 cut(s) 246, 596
XapI RAATTY 3 cut(s) 91, 157, 575
XceI RCATGY 1 cut(s) 431
XmiI GTMKAC 1 cut(s) 527
XmnI GAANNNNTTC 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.