MD03G1198700.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002678
Physical Location & Seq
Reverse (-)
27160752 .. 27162780
2029 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1198700.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGGCTAAATGTCACACTGATGTTTTGCCTAAGGCTTTCTTCGTCGTCGTCTTCTCTCTCGTCACTTTTGGTTTCTTCGTCACTCTCTCAGAAGCTCAGATGGTTCCGGCCATATATGTGTTTGGAGACTCGTTAGTCGACGTTGGTAACAACGATTACCTAAAAATCTCTGTCGTCAAGGCAAATTTTCCTCATAATGGGGTTGATTATCCTGACAAAGTAGCAACTGGGAGGTTTGGCAATGGAAAAAACTCTGCAGATTTTATTGAGAACGAGAGGCACTTACGGTCTGTAGTATACAATCTTGTTATCTTTTGTTTGGCAGCTCAGAAAGTCGGGTTACCAACATCGCCACCATATCTGTCGCTTGTTTCCAAGTCCAACAAGCAAAAAGCATCCTTCGTCAACGGAGTTAGCTTCGCCTCCGGAGGAGCTGGAATCTTCAACGGCTCAGATGAACAATATCGTCAGTCGATACCTTTGTCATACCAAGTAAACTACTACTCAGAAGTGCATCAAAACATTGGGCTACAATTAGGAGCCAAGGGCGCCAATGACCATCTTTCAAAATCTCTGTTTTTAATTGTGATTGGAAGCAATGACATATTCGACTACGTTAAAAAACCCAAACTCCAAAATGAATACACACCTCAGCAGTACGCGGATTTGATGGTGTCGAATCTAAAGGAACAATTACAGAGAATACATGCTCTAGGAGCGCAAAAATTTGTAGTCATCGGGACTGGGCCGGTTGGATGCTGTCCGTCGCAAAGGAATTCAAGTACAGGAGAGTGCAATGATGGTGCAAACTCATTGTCTGTCATGTACAACGAAGCCCTTAAGTCCATGTTGCAGCAATTGAAACCGGCGTTGGGCATAAGCTACACCCTCTTTGACACTTACACTGTCTTGACTAACATCGTTCAAAACCCCGCTTCTTACGGATTTACAGAGGTTGAAGCTGCATGCTGTGGGATTGGGAAGCGTAATGCCAAGGGTCCTTGCACACCAATTTCATCTTTGTGCTCCAACAGGAGGGACCATGTCTTCTGGGATTTTTACCATCCTACACAGGCAACTCATGGCATCATAACGGATAAACTTTTCGATGGCCCTTCAGAATACTCATCCCCAATGACTGTGAAGGAGTTAATTGCTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

388

Amino Acids

42.35

Weight (kDa)

7.56

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 367 6.3e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
AccB1I GGYRCC 1 cut(s) 548
AccI GTMKAC 2 cut(s) 138, 297
AccII CGCG 1 cut(s) 662
AccIII TCCGGA 1 cut(s) 425
AciI CCGC 2 cut(s) 662, 933
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 3 cut(s) 184, 725, 775
AcuI CTGAAG 1 cut(s) 1101
AcyI GRCGYC 1 cut(s) 549
AfaI GTAC 3 cut(s) 659, 784, 827
AfiI CCNNNNNNNGG 3 cut(s) 197, 872, 1035
AflII CTTAAG 1 cut(s) 839
AgsI TTSAA 6 cut(s) 445, 567, 780, 862, 926, 959
AjuI GAANNNNNNNTTGG 2 cut(s) 854, 886
AluBI AGCT 6 cut(s) 95, 326, 417, 434, 882, 962
AluI AGCT 6 cut(s) 95, 326, 417, 434, 882, 962
Alw21I GWGCWC 1 cut(s) 1028
Alw26I GTCTC 1 cut(s) 120
Aor13HI TCCGGA 1 cut(s) 425
AoxI GGCC 3 cut(s) 108, 746, 1111
ApeKI GCWGC 3 cut(s) 323, 853, 962
ApoI RAATTY 3 cut(s) 184, 725, 775
ArsI GACNNNNNNTTYG 2 cut(s) 800, 832
AspLEI GCGC 2 cut(s) 551, 721
AspS9I GGNCC 4 cut(s) 746, 998, 1039, 1112
AvaII GGWCC 2 cut(s) 998, 1039
AxyI CCTNAGG 1 cut(s) 30
BaeI ACNNNNGTAYC 2 cut(s) 467, 500
BanI GGYRCC 1 cut(s) 548
BbsI GAAGAC 2 cut(s) 43, 1039
Bbv12I GWGCWC 1 cut(s) 1028
BbvCI CCTCAGC 1 cut(s) 651
BbvI GCAGC 3 cut(s) 335, 865, 949
BccI CCATC 6 cut(s) 94, 567, 664, 794, 1071, 1103
BceAI ACGGC 1 cut(s) 463
BcoDI GTCTC 1 cut(s) 120
BfaI CTAG 1 cut(s) 713
BfmI CTRYAG 2 cut(s) 255, 291
BfoI RGCGCY 1 cut(s) 552
BfrI CTTAAG 1 cut(s) 839
BisI GCNGC 3 cut(s) 324, 854, 963
BlsI GCNGC 3 cut(s) 325, 855, 964
Bme18I GGWCC 2 cut(s) 998, 1039
BmgT120I GGNCC 4 cut(s) 746, 998, 1039, 1112
BmiI GGNNCC 5 cut(s) 105, 541, 550, 999, 1040
BmrI ACTGGG 2 cut(s) 237, 753
BmsI GCATC 4 cut(s) 404, 523, 746, 1095
BmuI ACTGGG 2 cut(s) 237, 753
BpiI GAAGAC 2 cut(s) 43, 1039
Bpu10I CCTNAGC 1 cut(s) 651
BsaHI GRCGYC 1 cut(s) 549
BsaJI CCNNGG 2 cut(s) 543, 993
BsaWI WCCGGW 1 cut(s) 425
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 872, 1035
Bse118I RCCGGY 2 cut(s) 748, 865
Bse1I ACTGG 2 cut(s) 232, 748
Bse21I CCTNAGG 1 cut(s) 30
Bse3DI GCAATG 3 cut(s) 247, 604, 802
BseAI TCCGGA 1 cut(s) 425
BseDI CCNNGG 2 cut(s) 543, 993
BseGI GGATG 4 cut(s) 395, 761, 1063, 1127
BseLI CCNNNNNNNGG 3 cut(s) 197, 872, 1035
BseMI GCAATG 3 cut(s) 247, 604, 802
BseMII CTCAG 6 cut(s) 102, 110, 341, 465, 519, 665
BseNI ACTGG 2 cut(s) 232, 748
BseRI GAGGAG 1 cut(s) 444
BseXI GCAGC 3 cut(s) 335, 865, 949
Bsh1236I CGCG 1 cut(s) 662
BshFI GGCC 3 cut(s) 110, 748, 1113
BshNI GGYRCC 1 cut(s) 548
BsiHKAI GWGCWC 1 cut(s) 1028
BsiSI CCGG 4 cut(s) 107, 426, 749, 866
BslFI GGGAC 2 cut(s) 754, 1052
BslI CCNNNNNNNGG 3 cut(s) 197, 872, 1035
BsmAI GTCTC 1 cut(s) 120
BsmFI GGGAC 2 cut(s) 754, 1052
BsnI GGCC 3 cut(s) 110, 748, 1113
Bsp1286I GDGCHC 1 cut(s) 1028
Bsp13I TCCGGA 1 cut(s) 425
Bsp1407I TGTACA 1 cut(s) 825
BspACI CCGC 2 cut(s) 662, 933
BspANI GGCC 3 cut(s) 110, 748, 1113
BspCNI CTCAG 6 cut(s) 101, 109, 340, 464, 518, 664
BspEI TCCGGA 1 cut(s) 425
BspFNI CGCG 1 cut(s) 662
BspLI GGNNCC 5 cut(s) 105, 541, 550, 999, 1040
BspMAI CTGCAG 1 cut(s) 259
BspT107I GGYRCC 1 cut(s) 548
BspTI CTTAAG 1 cut(s) 839
BsrDI GCAATG 3 cut(s) 247, 604, 802
BsrFI RCCGGY 2 cut(s) 748, 865
BsrGI TGTACA 1 cut(s) 825
BsrI ACTGG 2 cut(s) 232, 748
BssAI RCCGGY 2 cut(s) 748, 865
BssECI CCNNGG 2 cut(s) 543, 993
BssNAI GTATAC 1 cut(s) 298
BssNI GRCGYC 1 cut(s) 549
BssT1I CCWWGG 2 cut(s) 543, 993
Bst1107I GTATAC 1 cut(s) 298
Bst4CI ACNGT 3 cut(s) 288, 907, 1141
BstACI GRCGYC 1 cut(s) 549
BstAFI CTTAAG 1 cut(s) 839
BstAUI TGTACA 1 cut(s) 825
BstC8I GCNNGC 1 cut(s) 967
BstDEI CTNAG 7 cut(s) 30, 88, 96, 327, 451, 505, 651
BstEII GGTNACC 1 cut(s) 339
BstF5I GGATG 4 cut(s) 395, 761, 1063, 1127
BstFNI CGCG 1 cut(s) 662
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 2 cut(s) 551, 721
BstMAI GTCTC 1 cut(s) 120
BstMWI GCNNNNNNNGC 2 cut(s) 548, 716
BstNSI RCATGY 2 cut(s) 710, 969
BstPI GGTNACC 1 cut(s) 339
BstSFI CTRYAG 2 cut(s) 255, 291
BstUI CGCG 1 cut(s) 662
BstV1I GCAGC 3 cut(s) 335, 865, 949
BstV2I GAAGAC 2 cut(s) 43, 1039
BstZ17I GTATAC 1 cut(s) 298
Bsu36I CCTNAGG 1 cut(s) 30
BsuRI GGCC 3 cut(s) 110, 748, 1113
BtgZI GCGATG 1 cut(s) 333
BtsCI GGATG 4 cut(s) 395, 761, 1063, 1127
BtsIMutI CAGTG 2 cut(s) 15, 903
Cac8I GCNNGC 1 cut(s) 967
CfoI GCGC 2 cut(s) 551, 721
Cfr10I RCCGGY 2 cut(s) 748, 865
Cfr13I GGNCC 4 cut(s) 746, 998, 1039, 1112
Csp6I GTAC 3 cut(s) 658, 783, 826
CviAII CATG 6 cut(s) 707, 823, 847, 966, 1043, 1082
CviQI GTAC 3 cut(s) 658, 783, 826
DdeI CTNAG 7 cut(s) 30, 88, 96, 327, 451, 505, 651
DinI GGCGCC 1 cut(s) 550
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 1 cut(s) 108
Eco130I CCWWGG 2 cut(s) 543, 993
Eco47I GGWCC 2 cut(s) 998, 1039
Eco57I CTGAAG 1 cut(s) 1101
Eco81I CCTNAGG 1 cut(s) 30
Eco91I GGTNACC 1 cut(s) 339
EcoO109I RGGNCCY 1 cut(s) 998
EcoO65I GGTNACC 1 cut(s) 339
EcoRI GAATTC 1 cut(s) 775
EcoT14I CCWWGG 2 cut(s) 543, 993
EgeI GGCGCC 1 cut(s) 550
EheI GGCGCC 1 cut(s) 550
ErhI CCWWGG 2 cut(s) 543, 993
FaeI CATG 6 cut(s) 710, 826, 850, 969, 1046, 1085
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FaqI GGGAC 2 cut(s) 754, 1052
FatI CATG 6 cut(s) 706, 822, 846, 965, 1042, 1081
FauI CCCGC 1 cut(s) 940
FblI GTMKAC 2 cut(s) 138, 297
Fnu4HI GCNGC 3 cut(s) 324, 854, 963
FokI GGATG 4 cut(s) 382, 768, 1050, 1114
Fsp4HI GCNGC 3 cut(s) 324, 854, 963
FspBI CTAG 1 cut(s) 713
GlaI GCGC 2 cut(s) 550, 720
GluI GCNGC 3 cut(s) 324, 854, 963
HaeII RGCGCY 1 cut(s) 552
HaeIII GGCC 3 cut(s) 110, 748, 1113
HapII CCGG 4 cut(s) 107, 426, 749, 866
HhaI GCGC 2 cut(s) 551, 721
Hin1I GRCGYC 1 cut(s) 549
Hin1II CATG 6 cut(s) 710, 826, 850, 969, 1046, 1085
Hin6I GCGC 2 cut(s) 549, 719
HinP1I GCGC 2 cut(s) 549, 719
HincII GTYRAC 2 cut(s) 139, 406
HindII GTYRAC 2 cut(s) 139, 406
HinfI GANTC 3 cut(s) 128, 438, 679
HpaII CCGG 4 cut(s) 107, 426, 749, 866
Hpy166II GTNNAC 4 cut(s) 139, 298, 406, 496
Hpy188I TCNGA 6 cut(s) 91, 99, 330, 454, 508, 1120
Hpy188III TCNNGA 4 cut(s) 212, 426, 739, 910
Hpy8I GTNNAC 4 cut(s) 139, 298, 406, 496
Hpy99I CGWCG 4 cut(s) 47, 50, 143, 769
HpyAV CCTTC 3 cut(s) 409, 1125, 1138
HpyCH4III ACNGT 3 cut(s) 288, 907, 1141
HpyCH4IV ACGT 2 cut(s) 141, 615
HpyCH4V TGCA 7 cut(s) 257, 514, 795, 806, 853, 965, 1005
HpyF10VI GCNNNNNNNGC 2 cut(s) 548, 716
HpyF3I CTNAG 7 cut(s) 30, 88, 96, 327, 451, 505, 651
HpySE526I ACGT 2 cut(s) 141, 615
Hsp92I GRCGYC 1 cut(s) 549
Hsp92II CATG 6 cut(s) 710, 826, 850, 969, 1046, 1085
HspAI GCGC 2 cut(s) 549, 719
KasI GGCGCC 1 cut(s) 548
Kpn2I TCCGGA 1 cut(s) 425
LmnI GCTCC 4 cut(s) 431, 539, 716, 1031
Lsp1109I GCAGC 3 cut(s) 335, 865, 949
LweI GCATC 4 cut(s) 404, 523, 746, 1095
MaeI CTAG 1 cut(s) 713
MaeII ACGT 2 cut(s) 141, 615
MaeIII GTNAC 5 cut(s) 11, 61, 79, 146, 339
MboII GAAGA 5 cut(s) 31, 43, 67, 433, 1039
MfeI CAATTG 1 cut(s) 857
MhlI GDGCHC 1 cut(s) 1028
MluCI AATT 9 cut(s) 184, 533, 582, 692, 725, 775, 857, 1011, 1152
Mly113I GGCGCC 1 cut(s) 549
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 3 cut(s) 405, 733, 1053
MnlI CCTC 9 cut(s) 201, 225, 270, 422, 433, 660, 899, 946, 1029
MroI TCCGGA 1 cut(s) 425
MseI TTAA 4 cut(s) 581, 618, 840, 1151
MslI CAYNNNNRTG 3 cut(s) 18, 116, 1021
MspCI CTTAAG 1 cut(s) 839
MspI CCGG 4 cut(s) 107, 426, 749, 866
MunI CAATTG 1 cut(s) 857
MvnI CGCG 1 cut(s) 662
MwoI GCNNNNNNNGC 2 cut(s) 548, 716
NarI GGCGCC 1 cut(s) 549
NlaIII CATG 6 cut(s) 710, 826, 850, 969, 1046, 1085
NlaIV GGNNCC 5 cut(s) 105, 541, 550, 999, 1040
NmuCI GTSAC 3 cut(s) 11, 61, 79
NspI RCATGY 2 cut(s) 710, 969
PaeI GCATGC 1 cut(s) 969
PfeI GAWTC 2 cut(s) 438, 679
PflFI GACNNNGTC 1 cut(s) 1043
PkrI GCNGC 3 cut(s) 325, 855, 964
PleI GAGTC 1 cut(s) 122
PluTI GGCGCC 1 cut(s) 552
PpsI GAGTC 1 cut(s) 122
PpuMI RGGWCCY 1 cut(s) 998
Psp5II RGGWCCY 1 cut(s) 998
PspEI GGTNACC 1 cut(s) 339
PspN4I GGNNCC 5 cut(s) 105, 541, 550, 999, 1040
PspPI GGNCC 4 cut(s) 746, 998, 1039, 1112
PspPPI RGGWCCY 1 cut(s) 998
PstI CTGCAG 1 cut(s) 259
PsyI GACNNNGTC 1 cut(s) 1043
RsaI GTAC 3 cut(s) 659, 784, 827
RsaNI GTAC 3 cut(s) 658, 783, 826
RseI CAYNNNNRTG 3 cut(s) 18, 116, 1021
SalI GTCGAC 1 cut(s) 137
SaqAI TTAA 4 cut(s) 581, 618, 840, 1151
SatI GCNGC 3 cut(s) 324, 854, 963
Sau96I GGNCC 4 cut(s) 746, 998, 1039, 1112
SchI GAGTC 1 cut(s) 122
SduI GDGCHC 1 cut(s) 1028
SfaNI GCATC 4 cut(s) 404, 523, 746, 1095
SfcI CTRYAG 2 cut(s) 255, 291
SfoI GGCGCC 1 cut(s) 550
SinI GGWCC 2 cut(s) 998, 1039
SmiMI CAYNNNNRTG 3 cut(s) 18, 116, 1021
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
SphI GCATGC 1 cut(s) 969
Sse9I AATT 9 cut(s) 184, 533, 582, 692, 725, 775, 857, 1011, 1152
SsiI CCGC 2 cut(s) 662, 933
SspDI GGCGCC 1 cut(s) 548
SspMI CTAG 1 cut(s) 713
StyI CCWWGG 2 cut(s) 543, 993
TaaI ACNGT 3 cut(s) 288, 907, 1141
TaiI ACGT 2 cut(s) 144, 618
TaqI TCGA 5 cut(s) 138, 473, 609, 677, 1107
TasI AATT 9 cut(s) 184, 533, 582, 692, 725, 775, 857, 1011, 1152
TatI WGTACW 2 cut(s) 782, 825
TfiI GAWTC 2 cut(s) 438, 679
Tru1I TTAA 4 cut(s) 581, 618, 840, 1151
Tru9I TTAA 4 cut(s) 581, 618, 840, 1151
TscAI CASTG 2 cut(s) 22, 910
TseFI GTSAC 3 cut(s) 11, 61, 79
TseI GCWGC 3 cut(s) 323, 853, 962
Tsp45I GTSAC 3 cut(s) 11, 61, 79
TspDTI ATGAA 3 cut(s) 471, 654, 1005
TspGWI ACGGA 4 cut(s) 423, 753, 957, 1109
TspRI CASTG 2 cut(s) 22, 910
Tth111I GACNNNGTC 1 cut(s) 1043
Vha464I CTTAAG 1 cut(s) 839
VpaK11BI GGWCC 2 cut(s) 998, 1039
XapI RAATTY 3 cut(s) 184, 725, 775
XceI RCATGY 2 cut(s) 710, 969
XmiI GTMKAC 2 cut(s) 138, 297
XspI CTAG 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.