MD03G1198800.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002678
Physical Location & Seq
Reverse (-)
27198620 .. 27201495
2876 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1198800.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGGCTTCCAGACTTTTCCTTCTCATGAGTCTGATCATCTCCTTTCTCTTCTCTCCCTCCAACCTTCTGGGATTCACTGTCACGGAGGCGCGTGTGGTTCCGGCCATGTATGTTTTCGGAGACTCGTTGGTAGATGTGGGCAACAACAATTACCTCCAGTTTTCTTTTGCCAAAGCAAACTTTCCTCACAATGGCCTCGACTTTCCGACCAAACAGCCAACCGGCAGATTTTGTAACGGCAAGAATGCTGCAGATCTTCTCGCTGAGAAAATGGGCTTACCTACCATACCACCATATCTTTCTATGGCATCTAAATTGAACAAGAGCTATAACCTCTTCCTAAATGGTGTTAACTTTGCTTCTGGAGCTGCTAAAATCTTCAAAGAGGATCAACAATATCCCCAGTCAATATCTTTGGGAGAACAAGTAGATTATTATTTGTCAGTGAAGGAAGGCCTTGGGCAACAGATAGGAACCTTCAAAACACAAAAGCATCTATCAAAATCTCTCTTCACCATTATCATTGGAAGCAATGACATCTACACCTACTTTGACTCCTCCACCAGTAATAGGAGCACACCTCAGCAGTATGTTAACTCCCTGGTTCTCACACTTAAACAACAAGTGAAGCGTTTATATGATTATGGCGCTCGTAAATTTGTCATCGTTGGAATTGCGCTTATCGGGTGCACCCCGTCAGAAAGAAATGAGCGATCCGATCAAGAATGCAAAGCTGACGTTAATCAGTTGTCAGCTAAGTACAATCAAGTTCTTATATCAATGTTGAAGAACTTGAAATCGGAGCTCAAGGGCATAAACTACTCGTACTTTGATGGTTACAGCGTCATGCACAACTTCATTCAGAATCCAACAGCTTACGGATTTGCTGAGGTTAAAGCTGCTTGCTGTGGTCTTGGGAAGCTTAATGCGGATGCTCCATGCCTACCATTCGCAACGTACTGCAGCAACAGAAGCAACCATCTCTTCTGGGACAGAACTCATCCAACAGAGGCAGCACATCGCATCTTTGTGGACTATATGCTTTCTGGTCCCTTACAATACACGTTCCCAATTAACGTGAAAAAGCTAGTTTCCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

367

Amino Acids

40.89

Weight (kDa)

9.04

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 37 - 346 4.9e-36 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 91
AciI CCGC 1 cut(s) 929
AclWI GGATC 2 cut(s) 396, 708
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 1 cut(s) 656
AfaI GTAC 3 cut(s) 761, 827, 959
AfiI CCNNNNNNNGG 2 cut(s) 191, 570
AflIII ACRYGT 1 cut(s) 1062
AgsI TTSAA 5 cut(s) 319, 382, 481, 787, 796
AjnI CCWGG 1 cut(s) 600
AluBI AGCT 9 cut(s) 327, 368, 734, 755, 805, 875, 899, 922, 1087
AluI AGCT 9 cut(s) 327, 368, 734, 755, 805, 875, 899, 922, 1087
Alw21I GWGCWC 3 cut(s) 578, 692, 807
Alw26I GTCTC 1 cut(s) 114
Alw44I GTGCAC 1 cut(s) 688
AlwI GGATC 2 cut(s) 396, 708
AoxI GGCC 3 cut(s) 102, 193, 454
ApaLI GTGCAC 1 cut(s) 688
ApeKI GCWGC 5 cut(s) 248, 368, 899, 963, 1013
ApoI RAATTY 1 cut(s) 656
AspLEI GCGC 3 cut(s) 91, 650, 679
AspS9I GGNCC 1 cut(s) 1049
AsuHPI GGTGA 1 cut(s) 505
AvaII GGWCC 1 cut(s) 1049
BaeGI GKGCMC 1 cut(s) 692
BanII GRGCYC 1 cut(s) 807
Bbv12I GWGCWC 3 cut(s) 578, 692, 807
BbvCI CCTCAGC 2 cut(s) 582, 888
BbvI GCAGC 5 cut(s) 235, 355, 886, 975, 1025
BccI CCATC 2 cut(s) 827, 987
BceAI ACGGC 1 cut(s) 253
BciT130I CCWGG 1 cut(s) 602
BclI TGATCA 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 1 cut(s) 1088
BfmI CTRYAG 2 cut(s) 249, 961
BfoI RGCGCY 1 cut(s) 651
BglII AGATCT 1 cut(s) 253
BisI GCNGC 5 cut(s) 249, 369, 900, 964, 1014
BlsI GCNGC 5 cut(s) 250, 370, 901, 965, 1015
Bme1390I CCNGG 1 cut(s) 602
Bme18I GGWCC 1 cut(s) 1049
BmgT120I GGNCC 1 cut(s) 1049
BmiI GGNNCC 3 cut(s) 99, 475, 1051
BmrFI CCNGG 1 cut(s) 602
BmrI ACTGGG 1 cut(s) 397
BmsI GCATC 4 cut(s) 317, 502, 922, 1032
BmuI ACTGGG 1 cut(s) 397
BplI GAGNNNNNCTC 2 cut(s) 565, 597
BpmI CTGGAG 2 cut(s) 140, 384
Bpu10I CCTNAGC 2 cut(s) 582, 888
BpuEI CTTGAG 1 cut(s) 791
BsaJI CCNNGG 2 cut(s) 457, 600
BsaXI ACNNNNNCTCC 4 cut(s) 411, 441, 539, 569
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 570
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 3 cut(s) 157, 403, 564
Bse3DI GCAATG 1 cut(s) 538
BseBI CCWGG 1 cut(s) 602
BseDI CCNNGG 2 cut(s) 457, 600
BseGI GGATG 2 cut(s) 937, 1000
BseLI CCNNNNNNNGG 2 cut(s) 191, 570
BseMI GCAATG 1 cut(s) 538
BseMII CTCAG 3 cut(s) 255, 596, 879
BseNI ACTGG 3 cut(s) 157, 403, 564
BseRI GAGGAG 1 cut(s) 547
BseSI GKGCMC 1 cut(s) 692
BseXI GCAGC 5 cut(s) 235, 355, 886, 975, 1025
Bsh1236I CGCG 1 cut(s) 91
BshFI GGCC 3 cut(s) 104, 195, 456
BsiHKAI GWGCWC 3 cut(s) 578, 692, 807
BsiSI CCGG 2 cut(s) 101, 222
BslFI GGGAC 2 cut(s) 1004, 1035
BslI CCNNNNNNNGG 2 cut(s) 191, 570
BsmAI GTCTC 1 cut(s) 114
BsmFI GGGAC 2 cut(s) 1004, 1035
BsmI GAATGC 2 cut(s) 250, 731
BsnI GGCC 3 cut(s) 104, 195, 456
Bsp1286I GDGCHC 3 cut(s) 578, 692, 807
Bsp143I GATC 5 cut(s) 33, 253, 388, 713, 718
BspACI CCGC 1 cut(s) 929
BspANI GGCC 3 cut(s) 104, 195, 456
BspCNI CTCAG 3 cut(s) 256, 595, 880
BspFNI CGCG 1 cut(s) 91
BspHI TCATGA 1 cut(s) 24
BspLI GGNNCC 3 cut(s) 99, 475, 1051
BspMAI CTGCAG 2 cut(s) 253, 965
BspPI GGATC 2 cut(s) 396, 708
BsrDI GCAATG 1 cut(s) 538
BsrFI RCCGGY 1 cut(s) 221
BsrI ACTGG 3 cut(s) 157, 403, 564
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 2 cut(s) 457, 600
BssMI GATC 5 cut(s) 33, 253, 388, 713, 718
BssT1I CCWWGG 1 cut(s) 457
Bst2UI CCWGG 1 cut(s) 602
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 4 cut(s) 53, 341, 515, 989
BstC8I GCNNGC 1 cut(s) 904
BstDEI CTNAG 4 cut(s) 264, 582, 756, 888
BstF5I GGATG 2 cut(s) 937, 1000
BstFNI CGCG 1 cut(s) 91
BstH2I RGCGCY 1 cut(s) 651
BstHHI GCGC 3 cut(s) 91, 650, 679
BstKTI GATC 5 cut(s) 36, 256, 391, 716, 721
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 5 cut(s) 33, 253, 388, 713, 718
BstMWI GCNNNNNNNGC 2 cut(s) 365, 972
BstNI CCWGG 1 cut(s) 602
BstSCI CCNGG 1 cut(s) 600
BstSFI CTRYAG 2 cut(s) 249, 961
BstSLI GKGCMC 1 cut(s) 692
BstUI CGCG 1 cut(s) 91
BstV1I GCAGC 5 cut(s) 235, 355, 886, 975, 1025
BstX2I RGATCY 1 cut(s) 253
BstXI CCANNNNNNTGG 1 cut(s) 67
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 3 cut(s) 104, 195, 456
BtgZI GCGATG 1 cut(s) 1004
BtsCI GGATG 2 cut(s) 937, 1000
BtsIMutI CAGTG 2 cut(s) 75, 450
Cac8I GCNNGC 1 cut(s) 904
CciI TCATGA 1 cut(s) 24
CfoI GCGC 3 cut(s) 91, 650, 679
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 1 cut(s) 1049
CseI GACGC 1 cut(s) 832
Csp6I GTAC 3 cut(s) 760, 826, 958
CviAII CATG 4 cut(s) 25, 106, 847, 939
CviQI GTAC 3 cut(s) 760, 826, 958
DdeI CTNAG 4 cut(s) 264, 582, 756, 888
DpnI GATC 5 cut(s) 35, 255, 390, 715, 720
DpnII GATC 5 cut(s) 33, 253, 388, 713, 718
EaeI YGGCCR 1 cut(s) 102
Eam1104I CTCTTC 4 cut(s) 53, 341, 515, 989
EarI CTCTTC 4 cut(s) 53, 341, 515, 989
Ecl136II GAGCTC 1 cut(s) 805
Eco130I CCWWGG 1 cut(s) 457
Eco147I AGGCCT 1 cut(s) 456
Eco24I GRGCYC 1 cut(s) 807
Eco47I GGWCC 1 cut(s) 1049
Eco53kI GAGCTC 1 cut(s) 805
EcoICRI GAGCTC 1 cut(s) 805
EcoRII CCWGG 1 cut(s) 600
EcoT14I CCWWGG 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 807
ErhI CCWWGG 1 cut(s) 457
FaeI CATG 4 cut(s) 28, 109, 850, 942
FaqI GGGAC 2 cut(s) 1004, 1035
FatI CATG 4 cut(s) 24, 105, 846, 938
FbaI TGATCA 1 cut(s) 33
Fnu4HI GCNGC 5 cut(s) 249, 369, 900, 964, 1014
FokI GGATG 2 cut(s) 944, 987
FriOI GRGCYC 1 cut(s) 807
Fsp4HI GCNGC 5 cut(s) 249, 369, 900, 964, 1014
FspBI CTAG 1 cut(s) 1088
GlaI GCGC 3 cut(s) 90, 649, 678
GluI GCNGC 5 cut(s) 249, 369, 900, 964, 1014
GsuI CTGGAG 2 cut(s) 140, 384
HaeII RGCGCY 1 cut(s) 651
HaeIII GGCC 3 cut(s) 104, 195, 456
HapII CCGG 2 cut(s) 101, 222
HgaI GACGC 1 cut(s) 832
HhaI GCGC 3 cut(s) 91, 650, 679
Hin1II CATG 4 cut(s) 28, 109, 850, 942
Hin6I GCGC 3 cut(s) 89, 648, 677
HinP1I GCGC 3 cut(s) 89, 648, 677
HincII GTYRAC 2 cut(s) 352, 595
HindII GTYRAC 2 cut(s) 352, 595
HindIII AAGCTT 1 cut(s) 920
HinfI GANTC 5 cut(s) 28, 72, 122, 554, 865
HpaI GTTAAC 2 cut(s) 352, 595
HpaII CCGG 2 cut(s) 101, 222
HphI GGTGA 1 cut(s) 505
Hpy166II GTNNAC 4 cut(s) 352, 595, 690, 1033
Hpy188I TCNGA 7 cut(s) 33, 119, 207, 700, 718, 802, 864
Hpy188III TCNNGA 4 cut(s) 9, 25, 363, 722
Hpy8I GTNNAC 4 cut(s) 352, 595, 690, 1033
HpyAV CCTTC 5 cut(s) 29, 74, 442, 446, 487
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 4 cut(s) 738, 956, 1064, 1077
HpyCH4V TGCA 5 cut(s) 251, 690, 729, 850, 963
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 972
HpyF3I CTNAG 4 cut(s) 264, 582, 756, 888
HpySE526I ACGT 4 cut(s) 738, 956, 1064, 1077
Hsp92II CATG 4 cut(s) 28, 109, 850, 942
HspAI GCGC 3 cut(s) 89, 648, 677
Ksp22I TGATCA 1 cut(s) 33
KspAI GTTAAC 2 cut(s) 352, 595
Kzo9I GATC 5 cut(s) 33, 253, 388, 713, 718
LmnI GCTCC 4 cut(s) 365, 573, 802, 940
Lsp1109I GCAGC 5 cut(s) 235, 355, 886, 975, 1025
LweI GCATC 4 cut(s) 317, 502, 922, 1032
MaeI CTAG 1 cut(s) 1088
MaeII ACGT 4 cut(s) 738, 956, 1064, 1077
MaeIII GTNAC 3 cut(s) 79, 233, 836
MalI GATC 5 cut(s) 35, 255, 390, 715, 720
MboI GATC 5 cut(s) 33, 253, 388, 713, 718
MboII GAAGA 7 cut(s) 40, 248, 328, 370, 502, 799, 976
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 3 cut(s) 578, 692, 807
MluCI AATT 5 cut(s) 148, 314, 656, 672, 1071
MlyI GAGTC 3 cut(s) 37, 116, 548
MmeI TCCRAC 5 cut(s) 84, 230, 649, 893, 1028
MseI TTAA 8 cut(s) 351, 594, 615, 741, 894, 924, 1074, 1099
MslI CAYNNNNRTG 1 cut(s) 1028
MspI CCGG 2 cut(s) 101, 222
MspR9I CCNGG 1 cut(s) 602
Mva1269I GAATGC 2 cut(s) 250, 731
MvaI CCWGG 1 cut(s) 602
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 2 cut(s) 365, 972
NdeII GATC 5 cut(s) 33, 253, 388, 713, 718
NlaIII CATG 4 cut(s) 28, 109, 850, 942
NlaIV GGNNCC 3 cut(s) 99, 475, 1051
NmuCI GTSAC 1 cut(s) 79
PagI TCATGA 1 cut(s) 24
PceI AGGCCT 1 cut(s) 456
PctI GAATGC 2 cut(s) 250, 731
PfeI GAWTC 2 cut(s) 72, 865
PkrI GCNGC 5 cut(s) 250, 370, 901, 965, 1015
PleI GAGTC 3 cut(s) 36, 116, 548
PpsI GAGTC 3 cut(s) 36, 116, 548
Psp124BI GAGCTC 1 cut(s) 807
Psp6I CCWGG 1 cut(s) 600
PspGI CCWGG 1 cut(s) 600
PspN4I GGNNCC 3 cut(s) 99, 475, 1051
PspPI GGNCC 1 cut(s) 1049
PstI CTGCAG 2 cut(s) 253, 965
PsuI RGATCY 1 cut(s) 253
RsaI GTAC 3 cut(s) 761, 827, 959
RsaNI GTAC 3 cut(s) 760, 826, 958
RseI CAYNNNNRTG 1 cut(s) 1028
SacI GAGCTC 1 cut(s) 807
SaqAI TTAA 8 cut(s) 351, 594, 615, 741, 894, 924, 1074, 1099
SatI GCNGC 5 cut(s) 249, 369, 900, 964, 1014
Sau3AI GATC 5 cut(s) 33, 253, 388, 713, 718
Sau96I GGNCC 1 cut(s) 1049
SchI GAGTC 3 cut(s) 37, 116, 548
ScrFI CCNGG 1 cut(s) 602
SduI GDGCHC 3 cut(s) 578, 692, 807
SfaNI GCATC 4 cut(s) 317, 502, 922, 1032
SfcI CTRYAG 2 cut(s) 249, 961
SinI GGWCC 1 cut(s) 1049
SmiMI CAYNNNNRTG 1 cut(s) 1028
SmlI CTYRAG 1 cut(s) 806
SmoI CTYRAG 1 cut(s) 806
Sse9I AATT 5 cut(s) 148, 314, 656, 672, 1071
SseBI AGGCCT 1 cut(s) 456
SsiI CCGC 1 cut(s) 929
SspMI CTAG 1 cut(s) 1088
SstI GAGCTC 1 cut(s) 807
StuI AGGCCT 1 cut(s) 456
StyD4I CCNGG 1 cut(s) 600
StyI CCWWGG 1 cut(s) 457
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 4 cut(s) 741, 959, 1067, 1080
TaqI TCGA 1 cut(s) 198
TasI AATT 5 cut(s) 148, 314, 656, 672, 1071
TatI WGTACW 1 cut(s) 759
TfiI GAWTC 2 cut(s) 72, 865
Tru1I TTAA 8 cut(s) 351, 594, 615, 741, 894, 924, 1074, 1099
Tru9I TTAA 8 cut(s) 351, 594, 615, 741, 894, 924, 1074, 1099
TscAI CASTG 2 cut(s) 82, 450
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 5 cut(s) 248, 368, 899, 963, 1013
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 1 cut(s) 847
TspGWI ACGGA 2 cut(s) 98, 894
TspRI CASTG 2 cut(s) 82, 450
VneI GTGCAC 1 cut(s) 688
VpaK11BI GGWCC 1 cut(s) 1049
XapI RAATTY 1 cut(s) 656
XspI CTAG 1 cut(s) 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.