FvH4_3g19741

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002678
Physical Location & Seq
Forward (+)
12915587 .. 12917977
2391 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g19741.t1

Sequence Viewer

Length: 1092 bp
ATGGCCTTCAGTTCTTTCCTTCAGAGTGTCTCCGTCTTCTTGATCTTCTTGCTCTGCGGGATCTCTCGTACAGTTACGGAGGCTCGTGTGATTCCGGCCATGTACGTTTTTGGGGACTCCCTAGTAGATGTGGGCAACAACAACTACCTCAAGTATTCATTTGCCAAAGCAAACTTTCCTCACAATGGAGTCGACTTTCCGACCAAAAAACCAACCGGCAGGTTCGGCAATGGCAAGAATGCTGCAGATCTTATTGCTGAGAAAATGGGGTTGCCGACAATACCACCATATCTCTCCATGTCATCCAAATCAAACAAGAGCAGAACACAGTTCCTCAACGGCGTTAATTTTGCATCTGGAGCTTCCAAAATCTTGAACGACATAGATCCACAATACCCCTTTTCCATACCATTGGAAAAACAAATAGACTACTACTTGGCAGTGCATAAAGACCTTGTGCAAGCGCTAGGAGCCTCTAGAGCACAGATTCATCTATCAAAATCTCTCTTCCTCATTATCACGGGAAGCAATGACATGTACAACTACTTTGACTCCTTAAGTAACAGCACACAACAGCAGTATGTGAACTCCCTTGTTCTCATCTTCAAAGAACAAGTCAAGCGGTTATATGATTACGGCGGACGAAAATTTTCGATTATTGGGGTTGGGGTTATCGGATGCACACCGTCGGAAAGGAATGAGCAGGCGCACGGAAAATGCAATGAAGATGTAAATCGATTGTCACTCAAGTATAATCAAGCACTTGTGTCCATGTTAAAGAATTTGGCATCAGAGCTCAAAGGCATAAACTACTCCTACTTTGATGGTTACAGCGTCATGCAGAACTTCATCCAGAAGCCAACAGCTCATGGATTTTCTGAGGTTAAAGCTGCTTGCTGCGGTCTAGGGAAGCTGAATGCGGATGCTCCTTGCCTGCCAATTGCGACTTACTGCAGCAACAGAAGCAACCATTTGTTCTGGGACAGAACGCACCCTACTGAGGCAGCTCATCGCAAGCTTGTGGATTATATTCTTTATGGTCCTTTACGATACACGTTTCCGGTCAATGTGAAAAAGCTAGTTGCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

364

Amino Acids

40.41

Weight (kDa)

9.23

Isoelectric Point (pI)

27.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 344 6.6e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 210
AccI GTMKAC 1 cut(s) 192
AciI CCGC 5 cut(s) 57, 622, 639, 900, 920
AclWI GGATC 2 cut(s) 68, 380
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 2 cut(s) 647, 781
AcuI CTGAAG 1 cut(s) 5
AfaI GTAC 3 cut(s) 70, 104, 539
AfeI AGCGCT 1 cut(s) 465
AfiI CCNNNNNNNGG 2 cut(s) 185, 1000
AflII CTTAAG 1 cut(s) 556
AflIII ACRYGT 2 cut(s) 534, 1053
AgsI TTSAA 2 cut(s) 376, 607
AluBI AGCT 8 cut(s) 362, 796, 866, 890, 913, 1007, 1018, 1078
AluI AGCT 8 cut(s) 362, 796, 866, 890, 913, 1007, 1018, 1078
Alw21I GWGCWC 2 cut(s) 484, 798
Alw26I GTCTC 1 cut(s) 34
AlwI GGATC 2 cut(s) 68, 380
Aor51HI AGCGCT 1 cut(s) 465
AoxI GGCC 2 cut(s) 3, 96
ApeKI GCWGC 5 cut(s) 242, 890, 897, 954, 1004
ApoI RAATTY 2 cut(s) 647, 781
ArsI GACNNNNNNTTYG 2 cut(s) 600, 632
Asp700I GAANNNNTTC 1 cut(s) 649
AspLEI GCGC 2 cut(s) 466, 709
AspS9I GGNCC 1 cut(s) 1040
AvaII GGWCC 1 cut(s) 1040
BanII GRGCYC 1 cut(s) 798
BauI CACGAG 1 cut(s) 84
BbsI GAAGAC 1 cut(s) 28
Bbv12I GWGCWC 2 cut(s) 484, 798
BbvI GCAGC 5 cut(s) 229, 877, 884, 966, 1016
BccI CCATC 1 cut(s) 818
BceAI ACGGC 2 cut(s) 355, 652
BcoDI GTCTC 1 cut(s) 34
BfaI CTAG 5 cut(s) 122, 467, 477, 905, 1079
BfmI CTRYAG 2 cut(s) 243, 952
BfoI RGCGCY 1 cut(s) 467
BfrI CTTAAG 1 cut(s) 556
BfuAI ACCTGC 1 cut(s) 210
BglII AGATCT 1 cut(s) 247
BisI GCNGC 5 cut(s) 243, 891, 898, 955, 1005
BlsI GCNGC 5 cut(s) 244, 892, 899, 956, 1006
Bme18I GGWCC 1 cut(s) 1040
BmgT120I GGNCC 1 cut(s) 1040
BmiI GGNNCC 1 cut(s) 472
BmsI GCATC 4 cut(s) 362, 668, 797, 913
BpiI GAAGAC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 378
BpuEI CTTGAG 2 cut(s) 134, 731
Bsa29I ATCGAT 1 cut(s) 736
BsaBI GATNNNNATC 1 cut(s) 732
BsaWI WCCGGW 1 cut(s) 1060
BsaXI ACNNNNNCTCC 2 cut(s) 536, 566
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 1000
Bse118I RCCGGY 1 cut(s) 215
Bse3DI GCAATG 3 cut(s) 235, 535, 727
Bse8I GATNNNNATC 1 cut(s) 732
BseCI ATCGAT 1 cut(s) 736
BseGI GGATG 4 cut(s) 302, 683, 849, 928
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 2 cut(s) 185, 1000
BseMI GCAATG 3 cut(s) 235, 535, 727
BseMII CTCAG 3 cut(s) 249, 870, 990
BseXI GCAGC 5 cut(s) 229, 877, 884, 966, 1016
BshFI GGCC 2 cut(s) 5, 98
BshVI ATCGAT 1 cut(s) 736
BsiHKAI GWGCWC 2 cut(s) 484, 798
BsiSI CCGG 3 cut(s) 95, 216, 1061
BslFI GGGAC 2 cut(s) 128, 995
BslI CCNNNNNNNGG 2 cut(s) 185, 1000
BsmAI GTCTC 1 cut(s) 34
BsmFI GGGAC 2 cut(s) 128, 995
BsmI GAATGC 2 cut(s) 244, 922
BsnI GGCC 2 cut(s) 5, 98
Bsp1286I GDGCHC 2 cut(s) 484, 798
Bsp1407I TGTACA 1 cut(s) 537
Bsp143I GATC 4 cut(s) 42, 60, 247, 385
BspACI CCGC 5 cut(s) 57, 622, 639, 900, 920
BspANI GGCC 2 cut(s) 5, 98
BspCNI CTCAG 3 cut(s) 250, 871, 991
BspDI ATCGAT 1 cut(s) 736
BspLI GGNNCC 1 cut(s) 472
BspMAI CTGCAG 2 cut(s) 247, 956
BspMI ACCTGC 1 cut(s) 210
BspPI GGATC 2 cut(s) 68, 380
BspTI CTTAAG 1 cut(s) 556
BsrDI GCAATG 3 cut(s) 235, 535, 727
BsrFI RCCGGY 1 cut(s) 215
BsrGI TGTACA 1 cut(s) 537
BssAI RCCGGY 1 cut(s) 215
BssMI GATC 4 cut(s) 42, 60, 247, 385
BssSI CACGAG 1 cut(s) 84
Bst2BI CACGAG 1 cut(s) 84
Bst4CI ACNGT 3 cut(s) 73, 330, 687
Bst6I CTCTTC 1 cut(s) 512
BstAFI CTTAAG 1 cut(s) 556
BstAUI TGTACA 1 cut(s) 537
BstC8I GCNNGC 5 cut(s) 462, 705, 895, 935, 1016
BstDEI CTNAG 3 cut(s) 258, 879, 999
BstF5I GGATG 4 cut(s) 302, 683, 849, 928
BstH2I RGCGCY 1 cut(s) 467
BstHHI GCGC 2 cut(s) 466, 709
BstKTI GATC 4 cut(s) 45, 63, 250, 388
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 4 cut(s) 42, 60, 247, 385
BstMWI GCNNNNNNNGC 5 cut(s) 225, 359, 470, 479, 963
BstNSI RCATGY 1 cut(s) 538
BstSFI CTRYAG 2 cut(s) 243, 952
BstV1I GCAGC 5 cut(s) 229, 877, 884, 966, 1016
BstV2I GAAGAC 1 cut(s) 28
BstX2I RGATCY 3 cut(s) 60, 247, 385
BstXI CCANNNNNNTGG 1 cut(s) 412
BstYI RGATCY 3 cut(s) 60, 247, 385
Bsu15I ATCGAT 1 cut(s) 736
BsuRI GGCC 2 cut(s) 5, 98
BsuTUI ATCGAT 1 cut(s) 736
BtgZI GCGATG 1 cut(s) 995
BtsCI GGATG 4 cut(s) 302, 683, 849, 928
BtsI GCAGTG 1 cut(s) 447
BtsIMutI CAGTG 1 cut(s) 447
BveI ACCTGC 1 cut(s) 210
Cac8I GCNNGC 5 cut(s) 462, 705, 895, 935, 1016
CfoI GCGC 2 cut(s) 466, 709
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 1040
ClaI ATCGAT 1 cut(s) 736
CseI GACGC 1 cut(s) 823
Csp6I GTAC 3 cut(s) 69, 103, 538
CviAII CATG 6 cut(s) 100, 298, 535, 772, 838, 869
CviQI GTAC 3 cut(s) 69, 103, 538
DdeI CTNAG 3 cut(s) 258, 879, 999
DpnI GATC 4 cut(s) 44, 62, 249, 387
DpnII GATC 4 cut(s) 42, 60, 247, 385
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 1 cut(s) 512
EarI CTCTTC 1 cut(s) 512
EciI GGCGGA 1 cut(s) 654
Ecl136II GAGCTC 1 cut(s) 796
Eco24I GRGCYC 1 cut(s) 798
Eco47I GGWCC 1 cut(s) 1040
Eco47III AGCGCT 1 cut(s) 465
Eco53kI GAGCTC 1 cut(s) 796
Eco57I CTGAAG 1 cut(s) 5
EcoICRI GAGCTC 1 cut(s) 796
EcoT38I GRGCYC 1 cut(s) 798
FaeI CATG 6 cut(s) 103, 301, 538, 775, 841, 872
FaqI GGGAC 2 cut(s) 128, 995
FatI CATG 6 cut(s) 99, 297, 534, 771, 837, 868
FauI CCCGC 1 cut(s) 50
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 5 cut(s) 243, 891, 898, 955, 1005
FokI GGATG 4 cut(s) 289, 690, 836, 935
FriOI GRGCYC 1 cut(s) 798
Fsp4HI GCNGC 5 cut(s) 243, 891, 898, 955, 1005
FspBI CTAG 5 cut(s) 122, 467, 477, 905, 1079
GlaI GCGC 2 cut(s) 465, 708
GluI GCNGC 5 cut(s) 243, 891, 898, 955, 1005
GsuI CTGGAG 1 cut(s) 378
HaeII RGCGCY 1 cut(s) 467
HaeIII GGCC 2 cut(s) 5, 98
HapII CCGG 3 cut(s) 95, 216, 1061
HgaI GACGC 1 cut(s) 823
HhaI GCGC 2 cut(s) 466, 709
Hin1II CATG 6 cut(s) 103, 301, 538, 775, 841, 872
Hin6I GCGC 2 cut(s) 464, 707
HinP1I GCGC 2 cut(s) 464, 707
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 1016
HinfI GANTC 5 cut(s) 91, 116, 189, 487, 551
HpaII CCGG 3 cut(s) 95, 216, 1061
Hpy166II GTNNAC 2 cut(s) 193, 586
Hpy188I TCNGA 6 cut(s) 24, 201, 677, 691, 793, 880
Hpy188III TCNNGA 5 cut(s) 40, 357, 373, 477, 853
Hpy8I GTNNAC 2 cut(s) 193, 586
Hpy99I CGWCG 1 cut(s) 691
HpyAV CCTTC 2 cut(s) 16, 29
HpyCH4III ACNGT 3 cut(s) 73, 330, 687
HpyCH4IV ACGT 2 cut(s) 105, 1055
HpyCH4V TGCA 8 cut(s) 245, 353, 445, 460, 681, 720, 841, 954
HpyF10VI GCNNNNNNNGC 5 cut(s) 225, 359, 470, 479, 963
HpyF3I CTNAG 3 cut(s) 258, 879, 999
HpySE526I ACGT 2 cut(s) 105, 1055
Hsp92II CATG 6 cut(s) 103, 301, 538, 775, 841, 872
HspAI GCGC 2 cut(s) 464, 707
Kzo9I GATC 4 cut(s) 42, 60, 247, 385
LmnI GCTCC 3 cut(s) 359, 470, 931
LpnPI CCDG 9 cut(s) 108, 205, 229, 342, 689, 866, 947, 964, 1074
Lsp1109I GCAGC 5 cut(s) 229, 877, 884, 966, 1016
LweI GCATC 4 cut(s) 362, 668, 797, 913
MaeI CTAG 5 cut(s) 122, 467, 477, 905, 1079
MaeII ACGT 2 cut(s) 105, 1055
MaeIII GTNAC 4 cut(s) 73, 560, 741, 827
MalI GATC 4 cut(s) 44, 62, 249, 387
MboI GATC 4 cut(s) 42, 60, 247, 385
MboII GAAGA 5 cut(s) 28, 37, 499, 595, 737
MfeI CAATTG 1 cut(s) 939
MflI RGATCY 3 cut(s) 60, 247, 385
MhlI GDGCHC 2 cut(s) 484, 798
MluCI AATT 4 cut(s) 346, 647, 781, 939
MlyI GAGTC 3 cut(s) 110, 198, 545
MmeI TCCRAC 2 cut(s) 224, 669
MnlI CCTC 8 cut(s) 73, 158, 189, 344, 484, 521, 874, 994
MroXI GAANNNNTTC 1 cut(s) 649
MseI TTAA 4 cut(s) 345, 557, 776, 885
MspCI CTTAAG 1 cut(s) 556
MspI CCGG 3 cut(s) 95, 216, 1061
MunI CAATTG 1 cut(s) 939
Mva1269I GAATGC 2 cut(s) 244, 922
MwoI GCNNNNNNNGC 5 cut(s) 225, 359, 470, 479, 963
NdeII GATC 4 cut(s) 42, 60, 247, 385
NlaIII CATG 6 cut(s) 103, 301, 538, 775, 841, 872
NlaIV GGNNCC 1 cut(s) 472
NmuCI GTSAC 1 cut(s) 741
NspI RCATGY 1 cut(s) 538
PciI ACATGT 1 cut(s) 534
PctI GAATGC 2 cut(s) 244, 922
PdmI GAANNNNTTC 1 cut(s) 649
PfeI GAWTC 2 cut(s) 91, 487
PkrI GCNGC 5 cut(s) 244, 892, 899, 956, 1006
PleI GAGTC 3 cut(s) 110, 197, 545
PpsI GAGTC 3 cut(s) 110, 197, 545
PscI ACATGT 1 cut(s) 534
Psp124BI GAGCTC 1 cut(s) 798
PspN4I GGNNCC 1 cut(s) 472
PspPI GGNCC 1 cut(s) 1040
PsrI GAACNNNNNNTAC 2 cut(s) 979, 1011
PstI CTGCAG 2 cut(s) 247, 956
PsuI RGATCY 3 cut(s) 60, 247, 385
RsaI GTAC 3 cut(s) 70, 104, 539
RsaNI GTAC 3 cut(s) 69, 103, 538
SacI GAGCTC 1 cut(s) 798
SalI GTCGAC 1 cut(s) 191
SaqAI TTAA 4 cut(s) 345, 557, 776, 885
SatI GCNGC 5 cut(s) 243, 891, 898, 955, 1005
Sau3AI GATC 4 cut(s) 42, 60, 247, 385
Sau96I GGNCC 1 cut(s) 1040
SchI GAGTC 3 cut(s) 110, 198, 545
SduI GDGCHC 2 cut(s) 484, 798
SfaNI GCATC 4 cut(s) 362, 668, 797, 913
SfcI CTRYAG 2 cut(s) 243, 952
SinI GGWCC 1 cut(s) 1040
SmlI CTYRAG 3 cut(s) 149, 556, 746
SmoI CTYRAG 3 cut(s) 149, 556, 746
Sse9I AATT 4 cut(s) 346, 647, 781, 939
SsiI CCGC 5 cut(s) 57, 622, 639, 900, 920
SspMI CTAG 5 cut(s) 122, 467, 477, 905, 1079
SstI GAGCTC 1 cut(s) 798
TaaI ACNGT 3 cut(s) 73, 330, 687
TaiI ACGT 2 cut(s) 108, 1058
TaqI TCGA 3 cut(s) 192, 653, 736
TasI AATT 4 cut(s) 346, 647, 781, 939
TatI WGTACW 1 cut(s) 537
TfiI GAWTC 2 cut(s) 91, 487
Tru1I TTAA 4 cut(s) 345, 557, 776, 885
Tru9I TTAA 4 cut(s) 345, 557, 776, 885
TscAI CASTG 1 cut(s) 447
TseFI GTSAC 1 cut(s) 741
TseI GCWGC 5 cut(s) 242, 890, 897, 954, 1004
Tsp45I GTSAC 1 cut(s) 741
TspDTI ATGAA 4 cut(s) 147, 479, 738, 838
TspGWI ACGGA 3 cut(s) 22, 92, 726
TspRI CASTG 1 cut(s) 447
Vha464I CTTAAG 1 cut(s) 556
VpaK11BI GGWCC 1 cut(s) 1040
XapI RAATTY 2 cut(s) 647, 781
XbaI TCTAGA 1 cut(s) 476
XceI RCATGY 1 cut(s) 538
XmiI GTMKAC 1 cut(s) 192
XmnI GAANNNNTTC 1 cut(s) 649
XspI CTAG 5 cut(s) 122, 467, 477, 905, 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.