pycom03g15030

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002678
Physical Location & Seq
Reverse (-)
16402944 .. 16405568
2625 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g15030.1

Sequence Viewer

Length: 1143 bp
ATGGGGGAGGGCAGTGTGAATGTGAGAGCAGCAGTTACAACCATGGCTTCCAGACTTTTCCTTCTCATGAGTCTGATCATCTCCTTTCTCTTCTCTCCCTCCAACCTTCTGGGATTCACTGTCACGGAGGCGCGTGTAGTTCCGGCCATGTATGTTTTCGGGGACTCGTTGGTAGATGTGGGCAACAACAATTACCTCCAGTTTTCTCTTGCCAAAGCAAACTTTCCTCACAATGGCCTCGACTTTCCGACCAAACAGCCAACCGGCAGATTTTGTAACGGCAAGAATGCTGCAGATCTTCTCGCTGAGAAAATGGGCTTACCTACCATACCACCATATCTTTCCATGGCATCTAAATTGAACAAGAGCTATAACCTCTTCCTAAATGGTGTTAACTTTGCTTCTGGAGCTGCTAAAATCTTCAAAGAGGATCAACAATATCCCCAGTCAATATCTTTGGGAGAACAAGTAGATTATTATTTGTCAGTGAAGGAAGGCCTTGGGCAACAGATAGGAACCTTCAAAGCACAAAAACATCTATCAAAATCTCTCTTCACCATTATCATTGGAAGCAATGACATCTACACCTACTTTGACTCCTCCACCAGTAATAGGAGCACACCTCAGCAATATGTTAACTCCATGGTTCTCACACTTAAACAACAAGTGAAGCGTTTATACGATTATGGCGCTCGTAAATTTGTCATCGTTGGAATTGCGCTTATCGGGTGCACCCCGTCAGAAAGAAATGAGCGATCCGATCAAGAATGCAAAGCTGACGTTAATCAGTTGTCAGCTAAGTACAATAAAGTTCTTATATCAATGTTGAAGAACTTGAAATCGGAGCTCAAGGGCATAAACTACTCCTACTTTGATGGTTACAGCGTCATGCACAACTTCATTCAGAATCCAACAGCTTACGGATTTGCTGAGGTTAAAGCTGCTTGCTGTGGTCTTGGGAAGCTTAATGCGGATGCTCCATGCCTACCATTCGCAACGTACTGCAGTAACAGAAGCAACCATCTCTTCTGGGACAGAACTCATCCAACAGAGGCAGCACATCGCATCTTTGTGGATTATATTCTTTCTGGTCCTTTACAATACACCTTCCCAATCAACGTGAAAAAGCTAGTTTCCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

381

Amino Acids

42.2

Weight (kDa)

9.1

Isoelectric Point (pI)

34.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 51 - 361 4.8e-36 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 133
AciI CCGC 1 cut(s) 971
AclWI GGATC 2 cut(s) 438, 750
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 1 cut(s) 698
AfaI GTAC 2 cut(s) 803, 1001
AfiI CCNNNNNNNGG 2 cut(s) 233, 612
AgsI TTSAA 5 cut(s) 361, 424, 523, 829, 838
AluBI AGCT 9 cut(s) 369, 410, 776, 797, 847, 917, 941, 964, 1129
AluI AGCT 9 cut(s) 369, 410, 776, 797, 847, 917, 941, 964, 1129
Alw21I GWGCWC 3 cut(s) 620, 734, 849
Alw44I GTGCAC 1 cut(s) 730
AlwI GGATC 2 cut(s) 438, 750
AoxI GGCC 3 cut(s) 144, 235, 496
ApaLI GTGCAC 1 cut(s) 730
ApeKI GCWGC 5 cut(s) 29, 290, 410, 941, 1055
ApoI RAATTY 1 cut(s) 698
AspLEI GCGC 3 cut(s) 133, 692, 721
AspS9I GGNCC 1 cut(s) 1091
AsuHPI GGTGA 1 cut(s) 547
AvaII GGWCC 1 cut(s) 1091
BaeGI GKGCMC 1 cut(s) 734
BanII GRGCYC 1 cut(s) 849
BarI GAAGNNNNNNTAC 2 cut(s) 662, 694
Bbv12I GWGCWC 3 cut(s) 620, 734, 849
BbvCI CCTCAGC 2 cut(s) 624, 930
BbvI GCAGC 5 cut(s) 41, 277, 397, 928, 1067
BccI CCATC 2 cut(s) 869, 1029
BceAI ACGGC 1 cut(s) 295
BclI TGATCA 1 cut(s) 75
BfaI CTAG 1 cut(s) 1130
BfmI CTRYAG 2 cut(s) 291, 1003
BfoI RGCGCY 1 cut(s) 693
BglII AGATCT 1 cut(s) 295
BisI GCNGC 5 cut(s) 30, 291, 411, 942, 1056
BlsI GCNGC 5 cut(s) 31, 292, 412, 943, 1057
Bme18I GGWCC 1 cut(s) 1091
BmgT120I GGNCC 1 cut(s) 1091
BmiI GGNNCC 1 cut(s) 517
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 3 cut(s) 359, 964, 1074
BmuI ACTGGG 1 cut(s) 439
BplI GAGNNNNNCTC 2 cut(s) 607, 639
BpmI CTGGAG 2 cut(s) 182, 426
Bpu10I CCTNAGC 2 cut(s) 624, 930
BpuEI CTTGAG 1 cut(s) 833
BsaJI CCNNGG 4 cut(s) 42, 345, 499, 642
BsaXI ACNNNNNCTCC 4 cut(s) 453, 483, 581, 611
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 612
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 3 cut(s) 199, 445, 606
Bse3DI GCAATG 1 cut(s) 580
BseDI CCNNGG 4 cut(s) 42, 345, 499, 642
BseGI GGATG 2 cut(s) 979, 1042
BseLI CCNNNNNNNGG 2 cut(s) 233, 612
BseMI GCAATG 1 cut(s) 580
BseMII CTCAG 3 cut(s) 297, 638, 921
BseNI ACTGG 3 cut(s) 199, 445, 606
BseRI GAGGAG 1 cut(s) 589
BseSI GKGCMC 1 cut(s) 734
BseXI GCAGC 5 cut(s) 41, 277, 397, 928, 1067
Bsh1236I CGCG 1 cut(s) 133
BshFI GGCC 3 cut(s) 146, 237, 498
BsiHKAI GWGCWC 3 cut(s) 620, 734, 849
BsiSI CCGG 2 cut(s) 143, 264
BslFI GGGAC 2 cut(s) 176, 1046
BslI CCNNNNNNNGG 2 cut(s) 233, 612
BsmFI GGGAC 2 cut(s) 176, 1046
BsmI GAATGC 2 cut(s) 292, 773
BsnI GGCC 3 cut(s) 146, 237, 498
Bsp1286I GDGCHC 3 cut(s) 620, 734, 849
Bsp143I GATC 5 cut(s) 75, 295, 430, 755, 760
Bsp19I CCATGG 3 cut(s) 42, 345, 642
BspACI CCGC 1 cut(s) 971
BspANI GGCC 3 cut(s) 146, 237, 498
BspCNI CTCAG 3 cut(s) 298, 637, 922
BspFNI CGCG 1 cut(s) 133
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 517
BspMAI CTGCAG 2 cut(s) 295, 1007
BspPI GGATC 2 cut(s) 438, 750
BsrDI GCAATG 1 cut(s) 580
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 3 cut(s) 199, 445, 606
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 4 cut(s) 42, 345, 499, 642
BssMI GATC 5 cut(s) 75, 295, 430, 755, 760
BssT1I CCWWGG 4 cut(s) 42, 345, 499, 642
Bst4CI ACNGT 1 cut(s) 121
Bst6I CTCTTC 4 cut(s) 95, 383, 557, 1031
BstC8I GCNNGC 1 cut(s) 946
BstDEI CTNAG 4 cut(s) 306, 624, 798, 930
BstDSI CCRYGG 3 cut(s) 42, 345, 642
BstF5I GGATG 2 cut(s) 979, 1042
BstFNI CGCG 1 cut(s) 133
BstH2I RGCGCY 1 cut(s) 693
BstHHI GCGC 3 cut(s) 133, 692, 721
BstKTI GATC 5 cut(s) 78, 298, 433, 758, 763
BstMBI GATC 5 cut(s) 75, 295, 430, 755, 760
BstMWI GCNNNNNNNGC 1 cut(s) 407
BstSFI CTRYAG 2 cut(s) 291, 1003
BstSLI GKGCMC 1 cut(s) 734
BstUI CGCG 1 cut(s) 133
BstV1I GCAGC 5 cut(s) 41, 277, 397, 928, 1067
BstX2I RGATCY 1 cut(s) 295
BstXI CCANNNNNNTGG 1 cut(s) 109
BstYI RGATCY 1 cut(s) 295
BsuRI GGCC 3 cut(s) 146, 237, 498
BtgI CCRYGG 3 cut(s) 42, 345, 642
BtgZI GCGATG 1 cut(s) 1046
BtsCI GGATG 2 cut(s) 979, 1042
BtsI GCAGTG 1 cut(s) 19
BtsIMutI CAGTG 3 cut(s) 19, 117, 492
Cac8I GCNNGC 1 cut(s) 946
CciI TCATGA 1 cut(s) 66
CfoI GCGC 3 cut(s) 133, 692, 721
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 1091
CseI GACGC 1 cut(s) 874
Csp6I GTAC 2 cut(s) 802, 1000
CviAII CATG 7 cut(s) 43, 67, 148, 346, 643, 889, 981
CviQI GTAC 2 cut(s) 802, 1000
DdeI CTNAG 4 cut(s) 306, 624, 798, 930
DpnI GATC 5 cut(s) 77, 297, 432, 757, 762
DpnII GATC 5 cut(s) 75, 295, 430, 755, 760
EaeI YGGCCR 1 cut(s) 144
Eam1104I CTCTTC 4 cut(s) 95, 383, 557, 1031
EarI CTCTTC 4 cut(s) 95, 383, 557, 1031
Ecl136II GAGCTC 1 cut(s) 847
Eco130I CCWWGG 4 cut(s) 42, 345, 499, 642
Eco147I AGGCCT 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 849
Eco47I GGWCC 1 cut(s) 1091
Eco53kI GAGCTC 1 cut(s) 847
EcoICRI GAGCTC 1 cut(s) 847
EcoT14I CCWWGG 4 cut(s) 42, 345, 499, 642
EcoT38I GRGCYC 1 cut(s) 849
ErhI CCWWGG 4 cut(s) 42, 345, 499, 642
FaeI CATG 7 cut(s) 46, 70, 151, 349, 646, 892, 984
FaqI GGGAC 2 cut(s) 176, 1046
FatI CATG 7 cut(s) 42, 66, 147, 345, 642, 888, 980
FbaI TGATCA 1 cut(s) 75
Fnu4HI GCNGC 5 cut(s) 30, 291, 411, 942, 1056
FokI GGATG 2 cut(s) 986, 1029
FriOI GRGCYC 1 cut(s) 849
Fsp4HI GCNGC 5 cut(s) 30, 291, 411, 942, 1056
FspBI CTAG 1 cut(s) 1130
GlaI GCGC 3 cut(s) 132, 691, 720
GluI GCNGC 5 cut(s) 30, 291, 411, 942, 1056
GsuI CTGGAG 2 cut(s) 182, 426
HaeII RGCGCY 1 cut(s) 693
HaeIII GGCC 3 cut(s) 146, 237, 498
HapII CCGG 2 cut(s) 143, 264
HgaI GACGC 1 cut(s) 874
HhaI GCGC 3 cut(s) 133, 692, 721
Hin1II CATG 7 cut(s) 46, 70, 151, 349, 646, 892, 984
Hin6I GCGC 3 cut(s) 131, 690, 719
HinP1I GCGC 3 cut(s) 131, 690, 719
HincII GTYRAC 2 cut(s) 394, 637
HindII GTYRAC 2 cut(s) 394, 637
HindIII AAGCTT 1 cut(s) 962
HinfI GANTC 5 cut(s) 70, 114, 164, 596, 907
HpaI GTTAAC 2 cut(s) 394, 637
HpaII CCGG 2 cut(s) 143, 264
HphI GGTGA 1 cut(s) 547
Hpy166II GTNNAC 3 cut(s) 394, 637, 732
Hpy188I TCNGA 6 cut(s) 75, 249, 742, 760, 844, 906
Hpy188III TCNNGA 4 cut(s) 51, 67, 405, 764
Hpy8I GTNNAC 3 cut(s) 394, 637, 732
HpyAV CCTTC 6 cut(s) 71, 116, 484, 488, 529, 1117
HpyCH4III ACNGT 1 cut(s) 121
HpyCH4IV ACGT 3 cut(s) 780, 998, 1119
HpyCH4V TGCA 5 cut(s) 293, 732, 771, 892, 1005
HpyF10VI GCNNNNNNNGC 1 cut(s) 407
HpyF3I CTNAG 4 cut(s) 306, 624, 798, 930
HpySE526I ACGT 3 cut(s) 780, 998, 1119
Hsp92II CATG 7 cut(s) 46, 70, 151, 349, 646, 892, 984
HspAI GCGC 3 cut(s) 131, 690, 719
Ksp22I TGATCA 1 cut(s) 75
KspAI GTTAAC 2 cut(s) 394, 637
Kzo9I GATC 5 cut(s) 75, 295, 430, 755, 760
LmnI GCTCC 4 cut(s) 407, 615, 844, 982
Lsp1109I GCAGC 5 cut(s) 41, 277, 397, 928, 1067
LweI GCATC 3 cut(s) 359, 964, 1074
MaeI CTAG 1 cut(s) 1130
MaeII ACGT 3 cut(s) 780, 998, 1119
MaeIII GTNAC 5 cut(s) 34, 121, 275, 878, 1007
MalI GATC 5 cut(s) 77, 297, 432, 757, 762
MboI GATC 5 cut(s) 75, 295, 430, 755, 760
MboII GAAGA 7 cut(s) 82, 290, 370, 412, 544, 841, 1018
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 3 cut(s) 620, 734, 849
MluCI AATT 4 cut(s) 190, 356, 698, 714
MlyI GAGTC 3 cut(s) 79, 158, 590
MmeI TCCRAC 5 cut(s) 126, 272, 691, 935, 1070
MseI TTAA 7 cut(s) 393, 636, 657, 783, 936, 966, 1141
MslI CAYNNNNRTG 1 cut(s) 1070
MspI CCGG 2 cut(s) 143, 264
Mva1269I GAATGC 2 cut(s) 292, 773
MvnI CGCG 1 cut(s) 133
MwoI GCNNNNNNNGC 1 cut(s) 407
NcoI CCATGG 3 cut(s) 42, 345, 642
NdeII GATC 5 cut(s) 75, 295, 430, 755, 760
NlaIII CATG 7 cut(s) 46, 70, 151, 349, 646, 892, 984
NlaIV GGNNCC 1 cut(s) 517
NmuCI GTSAC 1 cut(s) 121
PagI TCATGA 1 cut(s) 66
PceI AGGCCT 1 cut(s) 498
PctI GAATGC 2 cut(s) 292, 773
PfeI GAWTC 2 cut(s) 114, 907
PkrI GCNGC 5 cut(s) 31, 292, 412, 943, 1057
PleI GAGTC 3 cut(s) 78, 158, 590
PpsI GAGTC 3 cut(s) 78, 158, 590
Psp124BI GAGCTC 1 cut(s) 849
PspN4I GGNNCC 1 cut(s) 517
PspPI GGNCC 1 cut(s) 1091
PstI CTGCAG 2 cut(s) 295, 1007
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 2 cut(s) 803, 1001
RsaNI GTAC 2 cut(s) 802, 1000
RseI CAYNNNNRTG 1 cut(s) 1070
SacI GAGCTC 1 cut(s) 849
SaqAI TTAA 7 cut(s) 393, 636, 657, 783, 936, 966, 1141
SatI GCNGC 5 cut(s) 30, 291, 411, 942, 1056
Sau3AI GATC 5 cut(s) 75, 295, 430, 755, 760
Sau96I GGNCC 1 cut(s) 1091
SchI GAGTC 3 cut(s) 79, 158, 590
SduI GDGCHC 3 cut(s) 620, 734, 849
SfaNI GCATC 3 cut(s) 359, 964, 1074
SfcI CTRYAG 2 cut(s) 291, 1003
SinI GGWCC 1 cut(s) 1091
SmiMI CAYNNNNRTG 1 cut(s) 1070
SmlI CTYRAG 1 cut(s) 848
SmoI CTYRAG 1 cut(s) 848
Sse9I AATT 4 cut(s) 190, 356, 698, 714
SseBI AGGCCT 1 cut(s) 498
SsiI CCGC 1 cut(s) 971
SspMI CTAG 1 cut(s) 1130
SstI GAGCTC 1 cut(s) 849
StuI AGGCCT 1 cut(s) 498
StyI CCWWGG 4 cut(s) 42, 345, 499, 642
TaaI ACNGT 1 cut(s) 121
TaiI ACGT 3 cut(s) 783, 1001, 1122
TaqI TCGA 1 cut(s) 240
TasI AATT 4 cut(s) 190, 356, 698, 714
TatI WGTACW 1 cut(s) 801
TfiI GAWTC 2 cut(s) 114, 907
Tru1I TTAA 7 cut(s) 393, 636, 657, 783, 936, 966, 1141
Tru9I TTAA 7 cut(s) 393, 636, 657, 783, 936, 966, 1141
TscAI CASTG 3 cut(s) 19, 124, 492
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 5 cut(s) 29, 290, 410, 941, 1055
Tsp45I GTSAC 1 cut(s) 121
TspDTI ATGAA 1 cut(s) 889
TspGWI ACGGA 2 cut(s) 140, 936
TspRI CASTG 3 cut(s) 19, 124, 492
VneI GTGCAC 1 cut(s) 730
VpaK11BI GGWCC 1 cut(s) 1091
XapI RAATTY 1 cut(s) 698
XspI CTAG 1 cut(s) 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.