Rh5CG257500

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0002678
Physical Location & Seq
Forward (+)
27359089 .. 27362112
3024 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG257500.1

Sequence Viewer

Length: 1380 bp
ATGGGATGCCGTGCACAAAGTGATAACAGTACTAGTATGAGAATTAATTTCCTAACAGCTGCATCTGCATCTGCATCTTATTATGATCGAATTTATGATGGAGCCCCAAATGACCAGTCGTCCGGTCAAATTATACTGTCTTTTCAGTTTTCACACACAAAAGGGCACAGACACGCTCATTTACTATCCTCTTTATGCATAAACCAAACTCACCACCGCCTCACAGAGGCTGCAAATAGAAATAGCATAGTGTTGGCTGTGAATGAGATCACAAACTTCATCTATACAATGGCCTGCAATTCCTTCCTTCCAAGTTTCTGCGTCTTCTTGCTTTTCTTCCTGTTCGGGGTTTCGAATACAGTTACGGAGGCTCGTGTGATTCCGGCCATGTTTGTTTTCGGAGACTCCCTAGTAGATGTGGGCAACAACAATTACCTCAGGTATTCATTTGCCAAAGCAAACTTTCCTCACAATGGGATCGACTTTCCGACCAGAAAACCAACCGGCAGGTTCGGCAATGGCAAGAATGCTGCCGATCTTATTGCTGAGAAAATGGGGTTGCCGACAATACCACCGTATCTCTCCATGTCATCCAAATCAAACAAGAGCATTACACAGTTCCTAAACGGCGTTAACTTTGCATCTGGAGCTTCCAAAATCTTAAACGACATAGATCCACAATATCCCTTTTCCATACCTTTGGAAAAACAAGTAGACTACTATTTGGCAGTGCATAAAGACCTAGCGCAAGGGCTAGGAGCCTCCAGAGCACAAATTTATTTATCAAAATCTCTCTTCCTCATTATCACGGGAAGCAATGACATCTACAACTACTTTGACTCGTTAAGTAACAGCACACAACAGCAGTACGTGAACTCCATTGTTCTCATCTTCAAAGAACAAGTGAAGCGGCTGTATGATTATGGTGCACGCAAATTCTCGATTGTTGGGGTTGGGGTTATCGGATGCACACCATCAGAAAGGAATGAGCAGGCGGACAGAAAATGCAATGAAGATACAAATCGATTGTCACTCAAGTACAATCAAGCACTCGTGTCCATGTTGAAGAATTTGGCATCAGAGCTCAGAGGCATAAACTACTCCTACTTTGATGGTTACAGCGTCATGCAGAACTTCATCCAAAAACCAACAGCTTATGGATTTTCTGAGGTTAAAGCTGCTTGCTGTGGTCTTGGGAAGCTCAATGCCGATGCTCCTTGCCTGCCATTTGCGACTTACTGCACCAACAGAAGCAACCATCTGTTCTGGGACAGAACGCATCCTACTGAGGCAGCTCATCGCAAGCTTGTGGATTATATGCTTTATGGTCCTTTACAATACACATTTCCACTCAATGTGAAAAAGCTAGTTGCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

459

Amino Acids

51.24

Weight (kDa)

8.98

Isoelectric Point (pI)

25.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 131 - 439 1.7e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 498
AccI GTMKAC 1 cut(s) 714
AciI CCGC 3 cut(s) 217, 910, 995
AclWI GGATC 2 cut(s) 485, 668
AcoI YGGCCR 1 cut(s) 384
AcsI RAATTY 4 cut(s) 90, 774, 935, 1069
AdeI CACNNNGTG 1 cut(s) 20
AfaI GTAC 3 cut(s) 31, 869, 1040
AfiI CCNNNNNNNGG 3 cut(s) 226, 346, 473
AgsI TTSAA 2 cut(s) 895, 1066
AhdI GACNNNNNGTC 1 cut(s) 118
AhlI ACTAGT 1 cut(s) 32
AluBI AGCT 9 cut(s) 59, 650, 1084, 1154, 1178, 1201, 1295, 1306, 1366
AluI AGCT 9 cut(s) 59, 650, 1084, 1154, 1178, 1201, 1295, 1306, 1366
Alw21I GWGCWC 4 cut(s) 16, 772, 931, 1086
Alw26I GTCTC 1 cut(s) 396
Alw44I GTGCAC 2 cut(s) 12, 927
AlwI GGATC 2 cut(s) 485, 668
AlwNI CAGNNNCTG 1 cut(s) 230
AoxI GGCC 2 cut(s) 291, 384
ApaLI GTGCAC 2 cut(s) 12, 927
ApeKI GCWGC 5 cut(s) 59, 230, 530, 1178, 1292
ApoI RAATTY 4 cut(s) 90, 774, 935, 1069
ArsI GACNNNNNNTTYG 4 cut(s) 101, 133, 1013, 1045
AseI ATTAAT 1 cut(s) 45
AspLEI GCGC 1 cut(s) 748
AspS9I GGNCC 1 cut(s) 1328
AsuHPI GGTGA 1 cut(s) 203
AsuII TTCGAA 1 cut(s) 353
AvaII GGWCC 1 cut(s) 1328
AxyI CCTNAGG 1 cut(s) 437
BaeGI GKGCMC 3 cut(s) 16, 168, 931
BaeI ACNNNNGTAYC 2 cut(s) 560, 593
BanII GRGCYC 2 cut(s) 106, 1086
BauI CACGAG 2 cut(s) 372, 1052
BbsI GAAGAC 1 cut(s) 316
Bbv12I GWGCWC 4 cut(s) 16, 772, 931, 1086
BbvI GCAGC 5 cut(s) 46, 217, 517, 1165, 1304
BccI CCATC 4 cut(s) 92, 982, 1106, 1266
BceAI ACGGC 1 cut(s) 643
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BcoDI GTCTC 1 cut(s) 396
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 5 cut(s) 33, 410, 743, 755, 1367
BfuAI ACCTGC 1 cut(s) 498
BisI GCNGC 6 cut(s) 60, 231, 531, 911, 1179, 1293
BlsI GCNGC 6 cut(s) 61, 232, 532, 912, 1180, 1294
BmcAI AGTACT 1 cut(s) 31
Bme18I GGWCC 1 cut(s) 1328
BmeRI GACNNNNNGTC 1 cut(s) 118
BmgT120I GGNCC 1 cut(s) 1328
BmiI GGNNCC 2 cut(s) 103, 760
BmsI GCATC 8 cut(s) 71, 77, 83, 650, 956, 1085, 1201, 1288
BpiI GAAGAC 1 cut(s) 316
BpmI CTGGAG 2 cut(s) 666, 748
Bpu14I TTCGAA 1 cut(s) 353
BpuEI CTTGAG 1 cut(s) 1019
Bsa29I ATCGAT 1 cut(s) 1024
BsaAI YACGTR 1 cut(s) 871
BsaBI GATNNNNATC 1 cut(s) 1020
BsaWI WCCGGW 1 cut(s) 122
BsaXI ACNNNNNCTCC 2 cut(s) 860, 890
Bsc4I CCNNNNNNNGG 3 cut(s) 226, 346, 473
Bse118I RCCGGY 1 cut(s) 503
Bse1I ACTGG 1 cut(s) 115
Bse21I CCTNAGG 1 cut(s) 437
Bse3DI GCAATG 3 cut(s) 523, 823, 1015
Bse8I GATNNNNATC 1 cut(s) 1020
BseCI ATCGAT 1 cut(s) 1024
BseGI GGATG 5 cut(s) 11, 590, 971, 1137, 1279
BseJI GATNNNNATC 1 cut(s) 1020
BseLI CCNNNNNNNGG 3 cut(s) 226, 346, 473
BseMI GCAATG 3 cut(s) 523, 823, 1015
BseMII CTCAG 5 cut(s) 451, 537, 1099, 1158, 1278
BseNI ACTGG 1 cut(s) 115
BseSI GKGCMC 3 cut(s) 16, 168, 931
BseXI GCAGC 5 cut(s) 46, 217, 517, 1165, 1304
BsgI GTGCAG 1 cut(s) 1225
BshFI GGCC 2 cut(s) 293, 386
BshVI ATCGAT 1 cut(s) 1024
BsiHKAI GWGCWC 4 cut(s) 16, 772, 931, 1086
BsiSI CCGG 3 cut(s) 123, 383, 504
BslFI GGGAC 1 cut(s) 1283
BslI CCNNNNNNNGG 3 cut(s) 226, 346, 473
BsmAI GTCTC 1 cut(s) 396
BsmFI GGGAC 1 cut(s) 1283
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 2 cut(s) 293, 386
Bsp119I TTCGAA 1 cut(s) 353
Bsp1286I GDGCHC 6 cut(s) 16, 106, 168, 772, 931, 1086
Bsp143I GATC 5 cut(s) 85, 267, 477, 535, 673
BspACI CCGC 3 cut(s) 217, 910, 995
BspANI GGCC 2 cut(s) 293, 386
BspCNI CTCAG 5 cut(s) 450, 538, 1098, 1159, 1279
BspDI ATCGAT 1 cut(s) 1024
BspLI GGNNCC 2 cut(s) 103, 760
BspMI ACCTGC 1 cut(s) 498
BspPI GGATC 2 cut(s) 485, 668
BspT104I TTCGAA 1 cut(s) 353
BsrDI GCAATG 3 cut(s) 523, 823, 1015
BsrFI RCCGGY 1 cut(s) 503
BsrI ACTGG 1 cut(s) 115
BssAI RCCGGY 1 cut(s) 503
BssMI GATC 5 cut(s) 85, 267, 477, 535, 673
BssSI CACGAG 2 cut(s) 372, 1052
Bst2BI CACGAG 2 cut(s) 372, 1052
Bst4CI ACNGT 5 cut(s) 29, 138, 361, 576, 618
Bst6I CTCTTC 1 cut(s) 800
BstBAI YACGTR 1 cut(s) 871
BstBI TTCGAA 1 cut(s) 353
BstC8I GCNNGC 6 cut(s) 295, 931, 993, 1183, 1223, 1304
BstDEI CTNAG 5 cut(s) 437, 546, 1085, 1167, 1287
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 5 cut(s) 11, 590, 971, 1137, 1279
BstHHI GCGC 1 cut(s) 748
BstKTI GATC 5 cut(s) 88, 270, 480, 538, 676
BstMAI GTCTC 1 cut(s) 396
BstMBI GATC 5 cut(s) 85, 267, 477, 535, 673
BstMWI GCNNNNNNNGC 4 cut(s) 65, 513, 647, 767
BstSLI GKGCMC 3 cut(s) 16, 168, 931
BstV1I GCAGC 5 cut(s) 46, 217, 517, 1165, 1304
BstV2I GAAGAC 1 cut(s) 316
BstX2I RGATCY 1 cut(s) 673
BstXI CCANNNNNNTGG 1 cut(s) 700
BstYI RGATCY 1 cut(s) 673
Bsu15I ATCGAT 1 cut(s) 1024
Bsu36I CCTNAGG 1 cut(s) 437
BsuRI GGCC 2 cut(s) 293, 386
BsuTUI ATCGAT 1 cut(s) 1024
BtgZI GCGATG 1 cut(s) 1283
BtsCI GGATG 5 cut(s) 11, 590, 971, 1137, 1279
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 1 cut(s) 735
BveI ACCTGC 1 cut(s) 498
Cac8I GCNNGC 6 cut(s) 295, 931, 993, 1183, 1223, 1304
CaiI CAGNNNCTG 1 cut(s) 230
CfoI GCGC 1 cut(s) 748
Cfr10I RCCGGY 1 cut(s) 503
Cfr13I GGNCC 1 cut(s) 1328
ClaI ATCGAT 1 cut(s) 1024
CseI GACGC 2 cut(s) 310, 1111
Csp6I GTAC 3 cut(s) 30, 868, 1039
CviAII CATG 4 cut(s) 388, 586, 1060, 1126
CviQI GTAC 3 cut(s) 30, 868, 1039
DdeI CTNAG 5 cut(s) 437, 546, 1085, 1167, 1287
DpnI GATC 5 cut(s) 87, 269, 479, 537, 675
DpnII GATC 5 cut(s) 85, 267, 477, 535, 673
DraIII CACNNNGTG 1 cut(s) 20
DriI GACNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 1 cut(s) 384
Eam1104I CTCTTC 1 cut(s) 800
Eam1105I GACNNNNNGTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 800
EciI GGCGGA 1 cut(s) 1010
Ecl136II GAGCTC 1 cut(s) 1084
Eco24I GRGCYC 2 cut(s) 106, 1086
Eco47I GGWCC 1 cut(s) 1328
Eco53kI GAGCTC 1 cut(s) 1084
Eco81I CCTNAGG 1 cut(s) 437
EcoICRI GAGCTC 1 cut(s) 1084
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoT22I ATGCAT 1 cut(s) 200
EcoT38I GRGCYC 2 cut(s) 106, 1086
FaeI CATG 4 cut(s) 391, 589, 1063, 1129
FaqI GGGAC 1 cut(s) 1283
FatI CATG 4 cut(s) 387, 585, 1059, 1125
FblI GTMKAC 1 cut(s) 714
Fnu4HI GCNGC 6 cut(s) 60, 231, 531, 911, 1179, 1293
FokI GGATG 5 cut(s) 18, 577, 978, 1124, 1266
FriOI GRGCYC 2 cut(s) 106, 1086
Fsp4HI GCNGC 6 cut(s) 60, 231, 531, 911, 1179, 1293
FspBI CTAG 5 cut(s) 33, 410, 743, 755, 1367
GlaI GCGC 1 cut(s) 747
GluI GCNGC 6 cut(s) 60, 231, 531, 911, 1179, 1293
GsuI CTGGAG 2 cut(s) 666, 748
HaeIII GGCC 2 cut(s) 293, 386
HapII CCGG 3 cut(s) 123, 383, 504
HgaI GACGC 2 cut(s) 310, 1111
HhaI GCGC 1 cut(s) 748
Hin1II CATG 4 cut(s) 391, 589, 1063, 1129
Hin6I GCGC 1 cut(s) 746
HinP1I GCGC 1 cut(s) 746
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HindIII AAGCTT 1 cut(s) 1304
HinfI GANTC 3 cut(s) 379, 404, 839
HpaI GTTAAC 1 cut(s) 634
HpaII CCGG 3 cut(s) 123, 383, 504
HphI GGTGA 1 cut(s) 203
Hpy166II GTNNAC 5 cut(s) 14, 634, 715, 874, 929
Hpy188I TCNGA 7 cut(s) 401, 489, 965, 979, 1081, 1088, 1168
Hpy188III TCNNGA 3 cut(s) 645, 765, 940
Hpy8I GTNNAC 5 cut(s) 14, 634, 715, 874, 929
HpyAV CCTTC 2 cut(s) 313, 317
HpyCH4III ACNGT 5 cut(s) 29, 138, 361, 576, 618
HpyCH4IV ACGT 1 cut(s) 870
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 513, 647, 767
HpyF3I CTNAG 5 cut(s) 437, 546, 1085, 1167, 1287
HpySE526I ACGT 1 cut(s) 870
Hsp92II CATG 4 cut(s) 391, 589, 1063, 1129
HspAI GCGC 1 cut(s) 746
KspAI GTTAAC 1 cut(s) 634
Kzo9I GATC 5 cut(s) 85, 267, 477, 535, 673
LmnI GCTCC 4 cut(s) 101, 647, 758, 1219
Lsp1109I GCAGC 5 cut(s) 46, 217, 517, 1165, 1304
LweI GCATC 8 cut(s) 71, 77, 83, 650, 956, 1085, 1201, 1288
MaeI CTAG 5 cut(s) 33, 410, 743, 755, 1367
MaeII ACGT 1 cut(s) 870
MaeIII GTNAC 4 cut(s) 361, 848, 1029, 1115
MalI GATC 5 cut(s) 87, 269, 479, 537, 675
MboI GATC 5 cut(s) 85, 267, 477, 535, 673
MboII GAAGA 6 cut(s) 316, 328, 787, 883, 1025, 1078
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 6 cut(s) 16, 106, 168, 772, 931, 1086
MluCI AATT 9 cut(s) 42, 46, 90, 129, 298, 430, 774, 935, 1069
MlyI GAGTC 2 cut(s) 398, 833
MmeI TCCRAC 1 cut(s) 512
Mph1103I ATGCAT 1 cut(s) 200
MseI TTAA 5 cut(s) 45, 633, 662, 845, 1173
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 3 cut(s) 123, 383, 504
Mva1269I GAATGC 1 cut(s) 532
MwoI GCNNNNNNNGC 4 cut(s) 65, 513, 647, 767
NdeII GATC 5 cut(s) 85, 267, 477, 535, 673
NlaIII CATG 4 cut(s) 391, 589, 1063, 1129
NlaIV GGNNCC 2 cut(s) 103, 760
NmuCI GTSAC 1 cut(s) 1029
NsiI ATGCAT 1 cut(s) 200
NspV TTCGAA 1 cut(s) 353
PctI GAATGC 1 cut(s) 532
PfeI GAWTC 1 cut(s) 379
PkrI GCNGC 6 cut(s) 61, 232, 532, 912, 1180, 1294
PleI GAGTC 2 cut(s) 398, 833
PpsI GAGTC 2 cut(s) 398, 833
Ppu21I YACGTR 1 cut(s) 871
PshBI ATTAAT 1 cut(s) 45
Psp124BI GAGCTC 1 cut(s) 1086
PspN4I GGNNCC 2 cut(s) 103, 760
PspPI GGNCC 1 cut(s) 1328
PsrI GAACNNNNNNTAC 2 cut(s) 1267, 1299
PstNI CAGNNNCTG 1 cut(s) 230
PsuI RGATCY 1 cut(s) 673
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 3 cut(s) 31, 869, 1040
RsaNI GTAC 3 cut(s) 30, 868, 1039
SacI GAGCTC 1 cut(s) 1086
SaqAI TTAA 5 cut(s) 45, 633, 662, 845, 1173
SatI GCNGC 6 cut(s) 60, 231, 531, 911, 1179, 1293
Sau3AI GATC 5 cut(s) 85, 267, 477, 535, 673
Sau96I GGNCC 1 cut(s) 1328
ScaI AGTACT 1 cut(s) 31
SchI GAGTC 2 cut(s) 398, 833
SduI GDGCHC 6 cut(s) 16, 106, 168, 772, 931, 1086
SfaNI GCATC 8 cut(s) 71, 77, 83, 650, 956, 1085, 1201, 1288
SfuI TTCGAA 1 cut(s) 353
SinI GGWCC 1 cut(s) 1328
SmlI CTYRAG 1 cut(s) 1034
SmoI CTYRAG 1 cut(s) 1034
SpeI ACTAGT 1 cut(s) 32
Sse9I AATT 9 cut(s) 42, 46, 90, 129, 298, 430, 774, 935, 1069
SsiI CCGC 3 cut(s) 217, 910, 995
SspMI CTAG 5 cut(s) 33, 410, 743, 755, 1367
SstI GAGCTC 1 cut(s) 1086
TaaI ACNGT 5 cut(s) 29, 138, 361, 576, 618
TaiI ACGT 1 cut(s) 873
TaqI TCGA 5 cut(s) 88, 353, 480, 941, 1024
TasI AATT 9 cut(s) 42, 46, 90, 129, 298, 430, 774, 935, 1069
TatI WGTACW 2 cut(s) 29, 1038
TauI GCSGC 1 cut(s) 913
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 5 cut(s) 45, 633, 662, 845, 1173
Tru9I TTAA 5 cut(s) 45, 633, 662, 845, 1173
TscAI CASTG 1 cut(s) 735
TseFI GTSAC 1 cut(s) 1029
TseI GCWGC 5 cut(s) 59, 230, 530, 1178, 1292
Tsp45I GTSAC 1 cut(s) 1029
TspDTI ATGAA 4 cut(s) 268, 435, 1026, 1126
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 1 cut(s) 735
VneI GTGCAC 2 cut(s) 12, 927
VpaK11BI GGWCC 1 cut(s) 1328
VspI ATTAAT 1 cut(s) 45
XagI CCTNNNNNAGG 1 cut(s) 224
XapI RAATTY 4 cut(s) 90, 774, 935, 1069
XmiI GTMKAC 1 cut(s) 714
XspI CTAG 5 cut(s) 33, 410, 743, 755, 1367
ZrmI AGTACT 1 cut(s) 31
Zsp2I ATGCAT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.