MD03G1198600.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002678
Physical Location & Seq
Reverse (-)
27150239 .. 27152830
2592 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1198600.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGCTAAATGTTACACTAATGTTTTGCCTAAGGCTTTCTTCGTCATCGTCTTCTCTCTCTTCACTTCTGGTTTCCTCGTCGCTCTCTCGGAGGCTCAGATGGTTCCGGCCATATACGTGTTCGGAGACTCGTTAGTCGACGTTGGTAACAACGATTACCTAACAATCTCTGTTTTCAAGGCAGATTTTCCTCATAATGGGGTTGATTATCCTGGCAAAGTAGCAACTGGGAGGTGGGGTAATGGAAAAAATTCTGCAGATTTTCTTGGGTTACCAACATCGCCACCGTATTTGTCACTTGTATCCAAGTCCAACAAGCAAAAAGCATCCTTCGTCAACGGAGTTAGCTTTGCCTCCGGAGGAGCTGGAATCTTCAACGGTTCAGATGAACAATATCGTCAGGCGATACCCTTGTCTTACCAAGTAAACTACTACTCAGAAGTGCATCAAAACATTGGGCTACAACTAGGCTCCAAGGGCGCCAAGGACCATCTTTCAAAATCTCTGTTTCTCATTGTGATTGGAAGCAATGACATCTTCGGCTACTTTAGAAATTCCCAACTCCAAAATGAATACACGCTGCAGCAGTACGCCAATTTGATGGTATCGAATCTAAAGGAACAATTACAGAGAATGCATGCTCTAGGCGCGCAGAAATTTGTAGTTATAGGGACTGGACCGGTTGGCTGCTGTCCGGCGCAAAGGAATTCAAGTACAGGAGAGTGCAATGATGTTGAAAACTCGATGTCCGTCATGTACAATGAAGCCCTTAAGTCCATGTTGCAGCAATTGAAACCGGCGTTGGGCATAAGCTACACCCTCTTTGACACTTATACTGTCTTGACTAACATCGTTCAAAACGCAGCTTCTTACGGATTTACAGAGGTTAGAGCTGCATGCTGTGGGATGGGGAAGCTTAACGCCGAGTTTCCTTGCACGCCAGTTTCGTCCTTGTGCTCCAACAGGAGGAACCATGTATTCTGGGATTTTTACCATCCTACACAGGCAACTCATGGCATCATAACGGATAAACTTTTTGATGGCCCTTCAAAATACTCATCCCCAATGACTGTGAAGGAGTTAATTGCTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

365

Amino Acids

39.84

Weight (kDa)

8.02

Isoelectric Point (pI)

33.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 344 1.4e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
AccB1I GGYRCC 1 cut(s) 479
AccI GTMKAC 1 cut(s) 138
AccII CGCG 1 cut(s) 650
AccIII TCCGGA 1 cut(s) 356
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 4 cut(s) 250, 553, 656, 706
AcyI GRCGYC 1 cut(s) 480
AfaI GTAC 3 cut(s) 590, 715, 758
AfiI CCNNNNNNNGG 3 cut(s) 197, 803, 966
AflII CTTAAG 1 cut(s) 770
AflIII ACRYGT 1 cut(s) 117
AgeI ACCGGT 1 cut(s) 679
AgsI TTSAA 8 cut(s) 178, 376, 498, 711, 737, 793, 857, 1050
AjnI CCWGG 1 cut(s) 211
AjuI GAANNNNNNNTTGG 2 cut(s) 785, 817
AluBI AGCT 6 cut(s) 348, 365, 813, 866, 893, 916
AluI AGCT 6 cut(s) 348, 365, 813, 866, 893, 916
Alw21I GWGCWC 1 cut(s) 959
Alw26I GTCTC 1 cut(s) 120
Aor13HI TCCGGA 1 cut(s) 356
AoxI GGCC 2 cut(s) 108, 1042
ApeKI GCWGC 6 cut(s) 580, 583, 687, 784, 863, 893
ApoI RAATTY 4 cut(s) 250, 553, 656, 706
AsiGI ACCGGT 1 cut(s) 679
Asp700I GAANNNNTTC 1 cut(s) 250
AspLEI GCGC 4 cut(s) 482, 650, 652, 700
AspS9I GGNCC 3 cut(s) 487, 677, 1043
AvaII GGWCC 2 cut(s) 487, 677
AxyI CCTNAGG 1 cut(s) 30
BaeI ACNNNNGTAYC 2 cut(s) 398, 431
BanI GGYRCC 1 cut(s) 479
BbsI GAAGAC 1 cut(s) 43
Bbv12I GWGCWC 1 cut(s) 959
BbvI GCAGC 6 cut(s) 567, 595, 674, 796, 875, 880
BccI CCATC 6 cut(s) 94, 498, 595, 901, 1002, 1034
BciT130I CCWGG 1 cut(s) 213
BciVI GTATCC 1 cut(s) 313
BcoDI GTCTC 1 cut(s) 120
BfaI CTAG 2 cut(s) 467, 644
BfmI CTRYAG 2 cut(s) 255, 581
BfoI RGCGCY 1 cut(s) 483
BfrI CTTAAG 1 cut(s) 770
BfuI GTATCC 1 cut(s) 313
BisI GCNGC 6 cut(s) 581, 584, 688, 785, 864, 894
BlsI GCNGC 6 cut(s) 582, 585, 689, 786, 865, 895
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 2 cut(s) 487, 677
BmgT120I GGNCC 3 cut(s) 487, 677, 1043
BmiI GGNNCC 4 cut(s) 105, 472, 481, 971
BmrFI CCNGG 1 cut(s) 213
BmrI ACTGGG 1 cut(s) 237
BmsI GCATC 3 cut(s) 335, 454, 1026
BmuI ACTGGG 1 cut(s) 237
BpiI GAAGAC 1 cut(s) 43
BsaAI YACGTR 1 cut(s) 118
BsaHI GRCGYC 1 cut(s) 480
BsaJI CCNNGG 2 cut(s) 474, 483
BsaWI WCCGGW 2 cut(s) 356, 679
BsaXI ACNNNNNCTCC 2 cut(s) 223, 253
Bsc4I CCNNNNNNNGG 3 cut(s) 197, 803, 966
Bse118I RCCGGY 2 cut(s) 679, 796
Bse1I ACTGG 3 cut(s) 232, 679, 941
Bse21I CCTNAGG 1 cut(s) 30
Bse3DI GCAATG 2 cut(s) 535, 733
BseAI TCCGGA 1 cut(s) 356
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 2 cut(s) 474, 483
BseGI GGATG 4 cut(s) 326, 912, 994, 1058
BseLI CCNNNNNNNGG 3 cut(s) 197, 803, 966
BseMI GCAATG 2 cut(s) 535, 733
BseMII CTCAG 2 cut(s) 110, 450
BseNI ACTGG 3 cut(s) 232, 679, 941
BsePI GCGCGC 1 cut(s) 648
BseRI GAGGAG 1 cut(s) 375
BseXI GCAGC 6 cut(s) 567, 595, 674, 796, 875, 880
Bsh1236I CGCG 1 cut(s) 650
BshFI GGCC 2 cut(s) 110, 1044
BshNI GGYRCC 1 cut(s) 479
BshTI ACCGGT 1 cut(s) 679
BsiHKAI GWGCWC 1 cut(s) 959
BsiSI CCGG 5 cut(s) 107, 357, 680, 695, 797
BslFI GGGAC 1 cut(s) 685
BslI CCNNNNNNNGG 3 cut(s) 197, 803, 966
BsmAI GTCTC 1 cut(s) 120
BsmFI GGGAC 1 cut(s) 685
BsmI GAATGC 1 cut(s) 639
BsnI GGCC 2 cut(s) 110, 1044
Bsp1286I GDGCHC 1 cut(s) 959
Bsp13I TCCGGA 1 cut(s) 356
Bsp1407I TGTACA 1 cut(s) 756
BspANI GGCC 2 cut(s) 110, 1044
BspCNI CTCAG 2 cut(s) 109, 449
BspEI TCCGGA 1 cut(s) 356
BspFNI CGCG 1 cut(s) 650
BspLI GGNNCC 4 cut(s) 105, 472, 481, 971
BspMAI CTGCAG 2 cut(s) 259, 585
BspT107I GGYRCC 1 cut(s) 479
BspTI CTTAAG 1 cut(s) 770
BsrDI GCAATG 2 cut(s) 535, 733
BsrFI RCCGGY 2 cut(s) 679, 796
BsrGI TGTACA 1 cut(s) 756
BsrI ACTGG 3 cut(s) 232, 679, 941
BssAI RCCGGY 2 cut(s) 679, 796
BssECI CCNNGG 2 cut(s) 474, 483
BssHII GCGCGC 1 cut(s) 648
BssNI GRCGYC 1 cut(s) 480
BssT1I CCWWGG 2 cut(s) 474, 483
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 4 cut(s) 288, 380, 838, 1072
Bst6I CTCTTC 1 cut(s) 65
BstACI GRCGYC 1 cut(s) 480
BstAFI CTTAAG 1 cut(s) 770
BstAUI TGTACA 1 cut(s) 756
BstBAI YACGTR 1 cut(s) 118
BstC8I GCNNGC 4 cut(s) 639, 650, 898, 938
BstDEI CTNAG 3 cut(s) 30, 96, 436
BstEII GGTNACC 1 cut(s) 270
BstF5I GGATG 4 cut(s) 326, 912, 994, 1058
BstFNI CGCG 1 cut(s) 650
BstH2I RGCGCY 1 cut(s) 483
BstHHI GCGC 4 cut(s) 482, 650, 652, 700
BstMAI GTCTC 1 cut(s) 120
BstMWI GCNNNNNNNGC 2 cut(s) 477, 647
BstNI CCWGG 1 cut(s) 213
BstNSI RCATGY 2 cut(s) 641, 900
BstPI GGTNACC 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 211
BstSFI CTRYAG 2 cut(s) 255, 581
BstUI CGCG 1 cut(s) 650
BstV1I GCAGC 6 cut(s) 567, 595, 674, 796, 875, 880
BstV2I GAAGAC 1 cut(s) 43
BstXI CCANNNNNNTGG 1 cut(s) 601
Bsu36I CCTNAGG 1 cut(s) 30
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 2 cut(s) 110, 1044
BtgZI GCGATG 1 cut(s) 264
BtsCI GGATG 4 cut(s) 326, 912, 994, 1058
Cac8I GCNNGC 4 cut(s) 639, 650, 898, 938
CfoI GCGC 4 cut(s) 482, 650, 652, 700
Cfr10I RCCGGY 2 cut(s) 679, 796
Cfr13I GGNCC 3 cut(s) 487, 677, 1043
Csp6I GTAC 3 cut(s) 589, 714, 757
CspAI ACCGGT 1 cut(s) 679
CspCI CAANNNNNGTGG 2 cut(s) 273, 308
CviAII CATG 6 cut(s) 638, 754, 778, 897, 974, 1013
CviQI GTAC 3 cut(s) 589, 714, 757
DdeI CTNAG 3 cut(s) 30, 96, 436
DinI GGCGCC 1 cut(s) 481
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Eco130I CCWWGG 2 cut(s) 474, 483
Eco47I GGWCC 2 cut(s) 487, 677
Eco81I CCTNAGG 1 cut(s) 30
Eco91I GGTNACC 1 cut(s) 270
EcoO65I GGTNACC 1 cut(s) 270
EcoRI GAATTC 1 cut(s) 706
EcoRII CCWGG 1 cut(s) 211
EcoT14I CCWWGG 2 cut(s) 474, 483
EcoT22I ATGCAT 1 cut(s) 639
EgeI GGCGCC 1 cut(s) 481
EheI GGCGCC 1 cut(s) 481
ErhI CCWWGG 2 cut(s) 474, 483
FaeI CATG 6 cut(s) 641, 757, 781, 900, 977, 1016
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FaqI GGGAC 1 cut(s) 685
FatI CATG 6 cut(s) 637, 753, 777, 896, 973, 1012
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 6 cut(s) 581, 584, 688, 785, 864, 894
FokI GGATG 4 cut(s) 313, 919, 981, 1045
Fsp4HI GCNGC 6 cut(s) 581, 584, 688, 785, 864, 894
FspBI CTAG 2 cut(s) 467, 644
GlaI GCGC 4 cut(s) 481, 649, 651, 699
GluI GCNGC 6 cut(s) 581, 584, 688, 785, 864, 894
HaeII RGCGCY 1 cut(s) 483
HaeIII GGCC 2 cut(s) 110, 1044
HapII CCGG 5 cut(s) 107, 357, 680, 695, 797
HhaI GCGC 4 cut(s) 482, 650, 652, 700
Hin1I GRCGYC 1 cut(s) 480
Hin1II CATG 6 cut(s) 641, 757, 781, 900, 977, 1016
Hin6I GCGC 4 cut(s) 480, 648, 650, 698
HinP1I GCGC 4 cut(s) 480, 648, 650, 698
HincII GTYRAC 2 cut(s) 139, 337
HindII GTYRAC 2 cut(s) 139, 337
HindIII AAGCTT 1 cut(s) 914
HinfI GANTC 3 cut(s) 128, 369, 610
HpaII CCGG 5 cut(s) 107, 357, 680, 695, 797
Hpy166II GTNNAC 3 cut(s) 139, 337, 427
Hpy188I TCNGA 5 cut(s) 91, 99, 125, 385, 439
Hpy188III TCNNGA 2 cut(s) 357, 841
Hpy8I GTNNAC 3 cut(s) 139, 337, 427
Hpy99I CGWCG 2 cut(s) 83, 143
HpyAV CCTTC 3 cut(s) 340, 1056, 1069
HpyCH4III ACNGT 4 cut(s) 288, 380, 838, 1072
HpyCH4IV ACGT 2 cut(s) 117, 141
HpyCH4V TGCA 8 cut(s) 257, 445, 583, 637, 726, 784, 896, 936
HpyF10VI GCNNNNNNNGC 2 cut(s) 477, 647
HpyF3I CTNAG 3 cut(s) 30, 96, 436
HpySE526I ACGT 2 cut(s) 117, 141
Hsp92I GRCGYC 1 cut(s) 480
Hsp92II CATG 6 cut(s) 641, 757, 781, 900, 977, 1016
HspAI GCGC 4 cut(s) 480, 648, 650, 698
KasI GGCGCC 1 cut(s) 479
Kpn2I TCCGGA 1 cut(s) 356
LmnI GCTCC 3 cut(s) 362, 476, 962
Lsp1109I GCAGC 6 cut(s) 567, 595, 674, 796, 875, 880
LweI GCATC 3 cut(s) 335, 454, 1026
MaeI CTAG 2 cut(s) 467, 644
MaeII ACGT 2 cut(s) 117, 141
MaeIII GTNAC 4 cut(s) 11, 146, 270, 294
MboII GAAGA 5 cut(s) 31, 43, 52, 364, 529
MfeI CAATTG 1 cut(s) 788
MhlI GDGCHC 1 cut(s) 959
MluCI AATT 8 cut(s) 250, 553, 595, 623, 656, 706, 788, 1083
Mly113I GGCGCC 1 cut(s) 480
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 2 cut(s) 336, 984
MnlI CCTC 9 cut(s) 85, 86, 201, 225, 353, 364, 830, 877, 960
Mph1103I ATGCAT 1 cut(s) 639
MroI TCCGGA 1 cut(s) 356
MroXI GAANNNNTTC 1 cut(s) 250
MseI TTAA 3 cut(s) 771, 918, 1082
MslI CAYNNNNRTG 1 cut(s) 116
MspCI CTTAAG 1 cut(s) 770
MspI CCGG 5 cut(s) 107, 357, 680, 695, 797
MspR9I CCNGG 1 cut(s) 213
MunI CAATTG 1 cut(s) 788
Mva1269I GAATGC 1 cut(s) 639
MvaI CCWGG 1 cut(s) 213
MvnI CGCG 1 cut(s) 650
MwoI GCNNNNNNNGC 2 cut(s) 477, 647
NarI GGCGCC 1 cut(s) 480
NlaIII CATG 6 cut(s) 641, 757, 781, 900, 977, 1016
NlaIV GGNNCC 4 cut(s) 105, 472, 481, 971
NmeAIII GCCGAG 1 cut(s) 949
NmuCI GTSAC 1 cut(s) 294
NsiI ATGCAT 1 cut(s) 639
NspI RCATGY 2 cut(s) 641, 900
PaeI GCATGC 2 cut(s) 641, 900
PauI GCGCGC 1 cut(s) 648
PcsI WCGNNNNNNNCGW 1 cut(s) 944
PctI GAATGC 1 cut(s) 639
PdmI GAANNNNTTC 1 cut(s) 250
PfeI GAWTC 2 cut(s) 369, 610
PinAI ACCGGT 1 cut(s) 679
PkrI GCNGC 6 cut(s) 582, 585, 689, 786, 865, 895
PleI GAGTC 1 cut(s) 122
PluTI GGCGCC 1 cut(s) 483
PpsI GAGTC 1 cut(s) 122
Ppu21I YACGTR 1 cut(s) 118
Psp6I CCWGG 1 cut(s) 211
PspEI GGTNACC 1 cut(s) 270
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 4 cut(s) 105, 472, 481, 971
PspPI GGNCC 3 cut(s) 487, 677, 1043
PstI CTGCAG 2 cut(s) 259, 585
PteI GCGCGC 1 cut(s) 648
RsaI GTAC 3 cut(s) 590, 715, 758
RsaNI GTAC 3 cut(s) 589, 714, 757
RseI CAYNNNNRTG 1 cut(s) 116
SalI GTCGAC 1 cut(s) 137
SaqAI TTAA 3 cut(s) 771, 918, 1082
SatI GCNGC 6 cut(s) 581, 584, 688, 785, 864, 894
Sau96I GGNCC 3 cut(s) 487, 677, 1043
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 1 cut(s) 959
SfaNI GCATC 3 cut(s) 335, 454, 1026
SfcI CTRYAG 2 cut(s) 255, 581
SfoI GGCGCC 1 cut(s) 481
SinI GGWCC 2 cut(s) 487, 677
SmiMI CAYNNNNRTG 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
SphI GCATGC 2 cut(s) 641, 900
Sse9I AATT 8 cut(s) 250, 553, 595, 623, 656, 706, 788, 1083
SspDI GGCGCC 1 cut(s) 479
SspMI CTAG 2 cut(s) 467, 644
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 2 cut(s) 474, 483
TaaI ACNGT 4 cut(s) 288, 380, 838, 1072
TaiI ACGT 2 cut(s) 120, 144
TaqI TCGA 3 cut(s) 138, 608, 743
TasI AATT 8 cut(s) 250, 553, 595, 623, 656, 706, 788, 1083
TatI WGTACW 2 cut(s) 713, 756
TfiI GAWTC 2 cut(s) 369, 610
Tru1I TTAA 3 cut(s) 771, 918, 1082
Tru9I TTAA 3 cut(s) 771, 918, 1082
TseFI GTSAC 1 cut(s) 294
TseI GCWGC 6 cut(s) 580, 583, 687, 784, 863, 893
Tsp45I GTSAC 1 cut(s) 294
TspDTI ATGAA 3 cut(s) 402, 585, 777
TspGWI ACGGA 4 cut(s) 354, 739, 888, 1040
Vha464I CTTAAG 1 cut(s) 770
VpaK11BI GGWCC 2 cut(s) 487, 677
XapI RAATTY 4 cut(s) 250, 553, 656, 706
XceI RCATGY 2 cut(s) 641, 900
XmiI GTMKAC 1 cut(s) 138
XmnI GAANNNNTTC 1 cut(s) 250
XspI CTAG 2 cut(s) 467, 644
Zsp2I ATGCAT 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.