Rorug05G0136900

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002678
Physical Location & Seq
Reverse (-)
12368206 .. 12372354
4149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0136900.1

Sequence Viewer

Length: 891 bp
ATGGCGGGTTCCGATCCAAACAAGCTGATGGCCAAAGCCGATAAGCTAACGAAGCTCAGTTTTACAAGGTGGAATGCTGATTGGAAAAGCGCTACTCTTCTGTATGAGCAAGCGGCTAATGGGTTTAGGATTGCCAAGGATTACGAGAAAGCAAAGGACGCACTCGAAAAAGCATCAAAAGGACAAGAGTTGATCTCCTCACCCTGGGATGCTGCAAAGCATATGGAGTCTGCTGCTGCTCTAGCAAAGGAGTTGGGCAAGTGGAATGAAGTTGCTGATTTTTACAGAAGGGCATCGGAATTATACACTGTGTGCGGAAGAGCACAGCCTGCATCAGATGCTCTTGCAAAAGGAGCCCGTACCCTTGAAGATCCTGTGCCTGAAGAAGCCATTCAACTTTACATTGAGGCCTGCACTATTCTTGAAGACGACGGCAAAGAGCAAATGGCCTTTGATCTATATCGAGATGTTACTAGTGTTTATATAAAACTTCAAAAGTATACAGATGCTGCGGCTTTCCTGTTGAGATGGGGCTTAGCAGCTGATAAGTGTAATGCTACCCATAGCCAGTGCAAGGCATATCTTAGTGCAATTCTCATATACCTGTATATTCATGATGTCAAGCAAGCAGAAAAATGCTACAACGATTGCTCCCAGGTCGATGCTTTTTTGGGCAATGATCATGGCCGATGTGCTAGTAAACTTCTTTCCGCATACACAGAAGGTGATATTGAAGAAATCAAACGACTTGCTCAGTCAAGCGCAGTTTCTCATCTTGATCACATGGTCATCAGACTCGCCAGGAAACTGCCTACAGGTGATGTCAGTGGACTAAAGACAAATGCGTCCAAGGAGCAAGAAGAAGAATTGGATGAAGATGACCTCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

296

Amino Acids

32.94

Weight (kDa)

5.22

Isoelectric Point (pI)

40.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNAP PF14938 5 - 264 8e-25 Soluble NSF attachment protein, SNAP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 844
AccI GTMKAC 1 cut(s) 500
AciI CCGC 5 cut(s) 5, 113, 315, 512, 711
AclWI GGATC 2 cut(s) 8, 365
AcoI YGGCCR 2 cut(s) 30, 685
AcuI CTGAAG 1 cut(s) 402
AdeI CACNNNGTG 1 cut(s) 312
AfaI GTAC 1 cut(s) 361
AfeI AGCGCT 1 cut(s) 91
AfiI CCNNNNNNNGG 2 cut(s) 204, 574
AgsI TTSAA 5 cut(s) 368, 395, 425, 494, 734
AhlI ACTAGT 1 cut(s) 473
AjnI CCWGG 3 cut(s) 203, 654, 800
AluBI AGCT 4 cut(s) 25, 46, 55, 542
AluI AGCT 4 cut(s) 25, 46, 55, 542
Alw21I GWGCWC 1 cut(s) 325
AlwI GGATC 2 cut(s) 8, 365
AlwNI CAGNNNCTG 1 cut(s) 509
Aor51HI AGCGCT 1 cut(s) 91
AoxI GGCC 4 cut(s) 30, 408, 447, 685
ApeKI GCWGC 5 cut(s) 212, 233, 236, 509, 539
Asp700I GAANNNNTTC 1 cut(s) 390
AspLEI GCGC 2 cut(s) 92, 764
AsuHPI GGTGA 3 cut(s) 192, 737, 830
BalI TGGCCA 1 cut(s) 32
BanII GRGCYC 1 cut(s) 358
BbsI GAAGAC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 325
BbvI GCAGC 5 cut(s) 199, 220, 223, 496, 551
BccI CCATC 2 cut(s) 22, 522
BceAI ACGGC 1 cut(s) 448
BciT130I CCWGG 3 cut(s) 205, 656, 802
BclI TGATCA 2 cut(s) 679, 778
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 3 cut(s) 242, 474, 696
BfmI CTRYAG 1 cut(s) 813
BfoI RGCGCY 1 cut(s) 93
BisI GCNGC 7 cut(s) 114, 213, 234, 237, 510, 513, 540
BlpI GCTNAGC 1 cut(s) 535
BlsI GCNGC 7 cut(s) 115, 214, 235, 238, 511, 514, 541
Bme1390I CCNGG 3 cut(s) 205, 656, 802
BmiI GGNNCC 2 cut(s) 10, 355
BmrFI CCNGG 3 cut(s) 205, 656, 802
BmsI GCATC 7 cut(s) 182, 199, 302, 328, 341, 496, 652
BpiI GAAGAC 1 cut(s) 432
BplI GAGNNNNNCTC 2 cut(s) 179, 211
Bpu1102I GCTNAGC 1 cut(s) 535
BsaBI GATNNNNATC 1 cut(s) 459
BsaJI CCNNGG 5 cut(s) 135, 203, 204, 654, 849
BsaXI ACNNNNNCTCC 2 cut(s) 635, 665
Bsc4I CCNNNNNNNGG 2 cut(s) 204, 574
Bse1I ACTGG 1 cut(s) 568
Bse3DI GCAATG 1 cut(s) 682
Bse8I GATNNNNATC 1 cut(s) 459
BseBI CCWGG 3 cut(s) 205, 656, 802
BseDI CCNNGG 5 cut(s) 135, 203, 204, 654, 849
BseGI GGATG 2 cut(s) 214, 877
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 2 cut(s) 204, 574
BseMI GCAATG 1 cut(s) 682
BseMII CTCAG 2 cut(s) 70, 767
BseNI ACTGG 1 cut(s) 568
BseRI GAGGAG 1 cut(s) 187
BseXI GCAGC 5 cut(s) 199, 220, 223, 496, 551
BsgI GTGCAG 1 cut(s) 397
BshFI GGCC 4 cut(s) 32, 410, 449, 687
BsiHKAI GWGCWC 1 cut(s) 325
BslI CCNNNNNNNGG 2 cut(s) 204, 574
BsmI GAATGC 1 cut(s) 79
BsnI GGCC 4 cut(s) 32, 410, 449, 687
Bsp1286I GDGCHC 2 cut(s) 325, 358
Bsp143I GATC 6 cut(s) 13, 192, 370, 454, 679, 778
Bsp1720I GCTNAGC 1 cut(s) 535
BspACI CCGC 5 cut(s) 5, 113, 315, 512, 711
BspANI GGCC 4 cut(s) 32, 410, 449, 687
BspCNI CTCAG 2 cut(s) 69, 766
BspHI TCATGA 1 cut(s) 613
BspLI GGNNCC 2 cut(s) 10, 355
BspPI GGATC 2 cut(s) 8, 365
BspQI GCTCTTC 1 cut(s) 313
BsrDI GCAATG 1 cut(s) 682
BsrI ACTGG 1 cut(s) 568
BssECI CCNNGG 5 cut(s) 135, 203, 204, 654, 849
BssMI GATC 6 cut(s) 13, 192, 370, 454, 679, 778
BssNAI GTATAC 1 cut(s) 501
BssT1I CCWWGG 2 cut(s) 135, 849
Bst1107I GTATAC 1 cut(s) 501
Bst2UI CCWGG 3 cut(s) 205, 656, 802
Bst4CI ACNGT 1 cut(s) 310
Bst6I CTCTTC 2 cut(s) 102, 313
BstAPI GCANNNNNTGC 2 cut(s) 329, 338
BstC8I GCNNGC 4 cut(s) 111, 330, 412, 627
BstDEI CTNAG 4 cut(s) 56, 535, 584, 753
BstF5I GGATG 2 cut(s) 214, 877
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 2 cut(s) 92, 764
BstKTI GATC 6 cut(s) 16, 195, 373, 457, 682, 781
BstMBI GATC 6 cut(s) 13, 192, 370, 454, 679, 778
BstMWI GCNNNNNNNGC 6 cut(s) 52, 158, 242, 329, 338, 353
BstNI CCWGG 3 cut(s) 205, 656, 802
BstSCI CCNGG 3 cut(s) 203, 654, 800
BstSFI CTRYAG 1 cut(s) 813
BstV1I GCAGC 5 cut(s) 199, 220, 223, 496, 551
BstV2I GAAGAC 1 cut(s) 432
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BstZ17I GTATAC 1 cut(s) 501
BsuRI GGCC 4 cut(s) 32, 410, 449, 687
BtsCI GGATG 2 cut(s) 214, 877
BtsIMutI CAGTG 3 cut(s) 306, 575, 832
Cac8I GCNNGC 4 cut(s) 111, 330, 412, 627
CaiI CAGNNNCTG 1 cut(s) 509
CciI TCATGA 1 cut(s) 613
CfoI GCGC 2 cut(s) 92, 764
CseI GACGC 2 cut(s) 167, 834
Csp6I GTAC 1 cut(s) 360
CviAII CATG 3 cut(s) 614, 683, 784
CviQI GTAC 1 cut(s) 360
DdeI CTNAG 4 cut(s) 56, 535, 584, 753
DpnI GATC 6 cut(s) 15, 194, 372, 456, 681, 780
DpnII GATC 6 cut(s) 13, 192, 370, 454, 679, 778
DraIII CACNNNGTG 1 cut(s) 312
DrdI GACNNNNNNGTC 1 cut(s) 844
DseDI GACNNNNNNGTC 1 cut(s) 844
EaeI YGGCCR 2 cut(s) 30, 685
Eam1104I CTCTTC 2 cut(s) 102, 313
EarI CTCTTC 2 cut(s) 102, 313
Eco130I CCWWGG 2 cut(s) 135, 849
Eco147I AGGCCT 1 cut(s) 410
Eco24I GRGCYC 1 cut(s) 358
Eco47III AGCGCT 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 402
EcoRII CCWGG 3 cut(s) 203, 654, 800
EcoT14I CCWWGG 2 cut(s) 135, 849
EcoT38I GRGCYC 1 cut(s) 358
ErhI CCWWGG 2 cut(s) 135, 849
FaeI CATG 3 cut(s) 617, 686, 787
FatI CATG 3 cut(s) 613, 682, 783
FauNDI CATATG 1 cut(s) 222
FbaI TGATCA 2 cut(s) 679, 778
FblI GTMKAC 1 cut(s) 500
Fnu4HI GCNGC 7 cut(s) 114, 213, 234, 237, 510, 513, 540
FokI GGATG 2 cut(s) 221, 884
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 7 cut(s) 114, 213, 234, 237, 510, 513, 540
FspBI CTAG 3 cut(s) 242, 474, 696
GlaI GCGC 2 cut(s) 91, 763
GluI GCNGC 7 cut(s) 114, 213, 234, 237, 510, 513, 540
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 4 cut(s) 32, 410, 449, 687
HgaI GACGC 2 cut(s) 167, 834
HhaI GCGC 2 cut(s) 92, 764
Hin1II CATG 3 cut(s) 617, 686, 787
Hin6I GCGC 2 cut(s) 90, 762
HinP1I GCGC 2 cut(s) 90, 762
HinfI GANTC 2 cut(s) 227, 795
HphI GGTGA 3 cut(s) 192, 737, 830
Hpy166II GTNNAC 3 cut(s) 501, 701, 830
Hpy188I TCNGA 4 cut(s) 13, 298, 337, 794
Hpy188III TCNNGA 4 cut(s) 422, 464, 614, 776
Hpy8I GTNNAC 3 cut(s) 501, 701, 830
Hpy99I CGWCG 1 cut(s) 434
HpyAV CCTTC 2 cut(s) 282, 716
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4V TGCA 6 cut(s) 215, 332, 347, 414, 573, 590
HpyF10VI GCNNNNNNNGC 6 cut(s) 52, 158, 242, 329, 338, 353
HpyF3I CTNAG 4 cut(s) 56, 535, 584, 753
Hsp92II CATG 3 cut(s) 617, 686, 787
HspAI GCGC 2 cut(s) 90, 762
Ksp22I TGATCA 2 cut(s) 679, 778
Kzo9I GATC 6 cut(s) 13, 192, 370, 454, 679, 778
LguI GCTCTTC 1 cut(s) 313
LmnI GCTCC 3 cut(s) 353, 656, 853
Lsp1109I GCAGC 5 cut(s) 199, 220, 223, 496, 551
LweI GCATC 7 cut(s) 182, 199, 302, 328, 341, 496, 652
MaeI CTAG 3 cut(s) 242, 474, 696
MaeIII GTNAC 1 cut(s) 469
MalI GATC 6 cut(s) 15, 194, 372, 456, 681, 780
MboI GATC 6 cut(s) 13, 192, 370, 454, 679, 778
MboII GAAGA 9 cut(s) 89, 330, 380, 395, 437, 746, 872, 875, 887
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 2 cut(s) 325, 358
MlsI TGGCCA 1 cut(s) 32
MluCI AATT 3 cut(s) 299, 591, 866
MluNI TGGCCA 1 cut(s) 32
MlyI GAGTC 2 cut(s) 236, 789
MnlI CCTC 2 cut(s) 208, 400
Mox20I TGGCCA 1 cut(s) 32
MroXI GAANNNNTTC 1 cut(s) 390
MscI TGGCCA 1 cut(s) 32
Msp20I TGGCCA 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 542
MspR9I CCNGG 3 cut(s) 205, 656, 802
Mva1269I GAATGC 1 cut(s) 79
MvaI CCWGG 3 cut(s) 205, 656, 802
MwoI GCNNNNNNNGC 6 cut(s) 52, 158, 242, 329, 338, 353
NdeI CATATG 1 cut(s) 222
NdeII GATC 6 cut(s) 13, 192, 370, 454, 679, 778
NlaIII CATG 3 cut(s) 617, 686, 787
NlaIV GGNNCC 2 cut(s) 10, 355
PagI TCATGA 1 cut(s) 613
PasI CCCWGGG 1 cut(s) 204
PceI AGGCCT 1 cut(s) 410
PciSI GCTCTTC 1 cut(s) 313
PctI GAATGC 1 cut(s) 79
PdmI GAANNNNTTC 1 cut(s) 390
PkrI GCNGC 7 cut(s) 115, 214, 235, 238, 511, 514, 541
PleI GAGTC 2 cut(s) 235, 789
PpsI GAGTC 2 cut(s) 235, 789
Psp6I CCWGG 3 cut(s) 203, 654, 800
PspGI CCWGG 3 cut(s) 203, 654, 800
PspN4I GGNNCC 2 cut(s) 10, 355
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 1 cut(s) 370
PvuII CAGCTG 1 cut(s) 542
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
SapI GCTCTTC 1 cut(s) 313
SatI GCNGC 7 cut(s) 114, 213, 234, 237, 510, 513, 540
Sau3AI GATC 6 cut(s) 13, 192, 370, 454, 679, 778
SchI GAGTC 2 cut(s) 236, 789
ScrFI CCNGG 3 cut(s) 205, 656, 802
SduI GDGCHC 2 cut(s) 325, 358
SfaNI GCATC 7 cut(s) 182, 199, 302, 328, 341, 496, 652
SfcI CTRYAG 1 cut(s) 813
SpeI ACTAGT 1 cut(s) 473
Sse9I AATT 3 cut(s) 299, 591, 866
SseBI AGGCCT 1 cut(s) 410
SsiI CCGC 5 cut(s) 5, 113, 315, 512, 711
SspMI CTAG 3 cut(s) 242, 474, 696
StuI AGGCCT 1 cut(s) 410
StyD4I CCNGG 3 cut(s) 203, 654, 800
StyI CCWWGG 2 cut(s) 135, 849
TaaI ACNGT 1 cut(s) 310
TaqI TCGA 3 cut(s) 165, 463, 660
TasI AATT 3 cut(s) 299, 591, 866
TauI GCSGC 2 cut(s) 116, 515
TscAI CASTG 3 cut(s) 313, 575, 832
TseI GCWGC 5 cut(s) 212, 233, 236, 509, 539
TspDTI ATGAA 3 cut(s) 282, 602, 888
TspRI CASTG 3 cut(s) 313, 575, 832
XmiI GTMKAC 1 cut(s) 500
XmnI GAANNNNTTC 1 cut(s) 390
XspI CTAG 3 cut(s) 242, 474, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.