AT5G60370
ERF Family

PD-(D/E)XK nuclease superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002756
Physical Location & Seq
Forward (+)
24282666 .. 24285061
2396 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G60370.1

Sequence Viewer

Length: 1242 bp
ATGGCCGAGTCACCCTCCGAGTCAACCTCCGACTCGCTTAGCACCACCACATCCACTAAGCCCGCTCAATCCGGGACTGTTTCTATTTCAAGCCCTCAAAGTCACCATGTCGTCTTCCCCGAGATCCCAATCGAGATCGTCAGTGAAGAAGAAATGGCTATTCTCGATGCAGCTCTTGCAGCCTCCCGCTCCATCCTCCCTTCCGTGATCCGTTCTGTTTCTCCGTCACGGATAACCGCCGGCGGAAGTCCCAAAACAATCCGCTCAATTACTCTCTTCTCCAAGAGGAAATTATCGGCTTGTTCAGATATCGAAGAGTCTTATCTACATCGATTTAGGAGAAATCAAGCTTTAGGTGTCACAGATCTCACCGGAACTGAATGGTGTGAGAAACAAATGGAGAATGTTCTTTGTTTTGGCAGGAGAAAAGTCAATAAAGCTATGAAAGTTGGTCAGGCTCGTCATTTGCAGCTTGAAGAAGAGGTAGTTAAAAAAGTGAGAGTAAAAGTTGAATCAAATGAAGATAAGTGGGCATTGAAGCTGTTGAATTCCATTGCTGGTGTTAATCAGTTCTTGTTTGAAGGACGAACTCGTGAATTGCTACTCTTAGGATTTGTTGGAGGTCAATGGATTGTGGGAATTATTGACGAGCTTAGAAAGGCGTATGCAGAAGAGTCTTCTGATAGCGGACCAATATTGATAGATACAAAGACTCGACTCCGTGATACACTTCCAGCTGAGCCACAGAGGAGGAATGGAAGGCTTCAGCTAATGCTTTACAAGCTTCTATGGGACACTATTGTGAAAGAAGGGTTTCCAACGGGACCTTTTTTCGACTACTTTTCTTTGAATCGCAATTATGTCTTATCCCAAGATGTCAGAGATCACATTGCTAATGCAGGTATCAAAGCTCAGACACTTGAAGAGATAGTTAGGTACTATGAAAACACTTTTCAGATGCTTCCGATAGCAAATAATCAACTATTACTAAAGTACGAGTTTCAGAAGGACCAATCTATAATAGCTGAAATTAGATTCAACCATGATCATGAATGGGTGATGAGCAAATATAGAGAAGTCATTGAGTTCTGGAGAAATGAACGAGAAGCTGGGTACACTCCTGAAGAAGAACGTTGGAAATGTCGGTATTGCCAATTTGCTAAGTCTTGCCCTGGAAACCCGAGTTTCGAATCTCCTAGCACAAGCTCACCAACAAGAGAGGCTCCTCCTTTACCAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

413

Amino Acids

46.97

Weight (kDa)

6.7

Isoelectric Point (pI)

63.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 107 - 222 2.7e-30 Exonuclease V - a 5' deoxyribonuclease
PDDEXK_1 PF12705 186 - 391 5.1e-07 PD-(D/E)XK nuclease superfamily
Exo5 PF09810 232 - 301 6.7e-06 Exonuclease V - a 5' deoxyribonuclease
Exo5 PF09810 305 - 390 8.7e-12 Exonuclease V - a 5' deoxyribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 890
AccBSI CCGCTC 3 cut(s) 65, 189, 264
AciI CCGC 6 cut(s) 63, 187, 237, 243, 262, 687
AclI AACGTT 1 cut(s) 1132
AclWI GGATC 2 cut(s) 118, 202
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 547
AcuI CTGAAG 2 cut(s) 749, 1143
AfaI GTAC 3 cut(s) 938, 995, 1115
AgsI TTSAA 9 cut(s) 90, 476, 512, 538, 547, 581, 850, 923, 1039
AjnI CCWGG 1 cut(s) 1171
AleI CACNNNNGTG 1 cut(s) 800
AlwI GGATC 2 cut(s) 118, 202
Ama87I CYCGRG 2 cut(s) 119, 1180
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 170, 179, 469
ApoI RAATTY 1 cut(s) 547
Asp700I GAANNNNTTC 1 cut(s) 813
AspS9I GGNCC 3 cut(s) 689, 824, 1009
AsuC2I CCSGG 1 cut(s) 73
AsuHPI GGTGA 5 cut(s) 3, 95, 361, 1069, 1200
AsuII TTCGAA 1 cut(s) 1188
AvaI CYCGRG 2 cut(s) 119, 1180
AvaII GGWCC 3 cut(s) 689, 824, 1009
BauI CACGAG 1 cut(s) 591
BbsI GAAGAC 2 cut(s) 106, 669
BbvI GCAGC 3 cut(s) 182, 191, 481
BccI CCATC 1 cut(s) 200
BciT130I CCWGG 1 cut(s) 1173
BclI TGATCA 1 cut(s) 1045
BcnI CCSGG 1 cut(s) 73
BfaI CTAG 1 cut(s) 1197
BfuAI ACCTGC 1 cut(s) 890
BglII AGATCT 1 cut(s) 364
BisI GCNGC 3 cut(s) 171, 180, 470
BlpI GCTNAGC 2 cut(s) 38, 738
BlsI GCNGC 3 cut(s) 172, 181, 471
Bme1390I CCNGG 2 cut(s) 73, 1173
Bme18I GGWCC 3 cut(s) 689, 824, 1009
BmeT110I CYCGRG 2 cut(s) 119, 1180
BmgT120I GGNCC 3 cut(s) 689, 824, 1009
BmiI GGNNCC 2 cut(s) 825, 1224
BmrFI CCNGG 2 cut(s) 73, 1173
BmsI GCATC 2 cut(s) 157, 948
BpiI GAAGAC 2 cut(s) 106, 669
BplI GAGNNNNNCTC 3 cut(s) 31, 11, 43
BpmI CTGGAG 1 cut(s) 1111
Bpu1102I GCTNAGC 2 cut(s) 38, 738
Bpu14I TTCGAA 1 cut(s) 1188
BpuMI CCSGG 1 cut(s) 73
Bsa29I ATCGAT 1 cut(s) 331
BsaBI GATNNNNATC 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 1171
BsaWI WCCGGW 1 cut(s) 371
Bse118I RCCGGY 1 cut(s) 239
Bse3DI GCAATG 2 cut(s) 552, 888
Bse8I GATNNNNATC 1 cut(s) 128
BseBI CCWGG 1 cut(s) 1173
BseCI ATCGAT 1 cut(s) 331
BseDI CCNNGG 1 cut(s) 1171
BseGI GGATG 2 cut(s) 50, 192
BseJI GATNNNNATC 1 cut(s) 128
BseMI GCAATG 2 cut(s) 552, 888
BseMII CTCAG 2 cut(s) 729, 926
BseRI GAGGAG 2 cut(s) 763, 1215
BseXI GCAGC 3 cut(s) 182, 191, 481
BseYI CCCAGC 1 cut(s) 1109
BshFI GGCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 331
BsiHKCI CYCGRG 2 cut(s) 119, 1180
BsiSI CCGG 3 cut(s) 72, 240, 372
BslFI GGGAC 4 cut(s) 88, 234, 806, 837
BsmFI GGGAC 4 cut(s) 88, 234, 806, 837
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 2 cut(s) 119, 1180
Bsp119I TTCGAA 1 cut(s) 1188
Bsp143I GATC 6 cut(s) 123, 135, 207, 364, 883, 1045
Bsp1720I GCTNAGC 2 cut(s) 38, 738
BspACI CCGC 6 cut(s) 63, 187, 237, 243, 262, 687
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 730, 925
BspDI ATCGAT 1 cut(s) 331
BspHI TCATGA 1 cut(s) 1048
BspLI GGNNCC 2 cut(s) 825, 1224
BspMI ACCTGC 1 cut(s) 890
BspPI GGATC 2 cut(s) 118, 202
BspT104I TTCGAA 1 cut(s) 1188
BsrBI CCGCTC 3 cut(s) 65, 189, 264
BsrDI GCAATG 2 cut(s) 552, 888
BsrFI RCCGGY 1 cut(s) 239
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 1 cut(s) 1171
BssMI GATC 6 cut(s) 123, 135, 207, 364, 883, 1045
BssSI CACGAG 1 cut(s) 591
Bst2BI CACGAG 1 cut(s) 591
Bst2UI CCWGG 1 cut(s) 1173
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 5 cut(s) 281, 309, 474, 666, 918
BstAPI GCANNNNNTGC 1 cut(s) 176
BstBI TTCGAA 1 cut(s) 1188
BstC8I GCNNGC 2 cut(s) 63, 241
BstDEI CTNAG 7 cut(s) 38, 57, 607, 653, 738, 912, 1161
BstF5I GGATG 2 cut(s) 50, 192
BstKTI GATC 6 cut(s) 126, 138, 210, 367, 886, 1048
BstMBI GATC 6 cut(s) 123, 135, 207, 364, 883, 1045
BstMWI GCNNNNNNNGC 3 cut(s) 176, 179, 781
BstNI CCWGG 1 cut(s) 1173
BstSCI CCNGG 2 cut(s) 71, 1171
BstV1I GCAGC 3 cut(s) 182, 191, 481
BstV2I GAAGAC 2 cut(s) 106, 669
BstX2I RGATCY 2 cut(s) 123, 364
BstYI RGATCY 2 cut(s) 123, 364
Bsu15I ATCGAT 1 cut(s) 331
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 331
BtsCI GGATG 2 cut(s) 50, 192
BtsIMutI CAGTG 1 cut(s) 148
BveI ACCTGC 1 cut(s) 890
Cac8I GCNNGC 2 cut(s) 63, 241
CciI TCATGA 1 cut(s) 1048
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 3 cut(s) 689, 824, 1009
ClaI ATCGAT 1 cut(s) 331
Csp6I GTAC 3 cut(s) 937, 994, 1114
CviAII CATG 3 cut(s) 107, 1043, 1049
CviQI GTAC 3 cut(s) 937, 994, 1114
DdeI CTNAG 7 cut(s) 38, 57, 607, 653, 738, 912, 1161
DpnI GATC 6 cut(s) 125, 137, 209, 366, 885, 1047
DpnII GATC 6 cut(s) 123, 135, 207, 364, 883, 1045
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 5 cut(s) 281, 309, 474, 666, 918
EarI CTCTTC 5 cut(s) 281, 309, 474, 666, 918
EciI GGCGGA 1 cut(s) 258
Eco32I GATATC 1 cut(s) 310
Eco47I GGWCC 3 cut(s) 689, 824, 1009
Eco57I CTGAAG 2 cut(s) 749, 1143
Eco88I CYCGRG 2 cut(s) 119, 1180
EcoO109I RGGNCCY 1 cut(s) 824
EcoRI GAATTC 1 cut(s) 547
EcoRII CCWGG 1 cut(s) 1171
EcoRV GATATC 1 cut(s) 310
FaeI CATG 3 cut(s) 110, 1046, 1052
FaqI GGGAC 4 cut(s) 88, 234, 806, 837
FatI CATG 3 cut(s) 106, 1042, 1048
FauI CCCGC 2 cut(s) 70, 194
FbaI TGATCA 1 cut(s) 1045
Fnu4HI GCNGC 3 cut(s) 171, 180, 470
FokI GGATG 2 cut(s) 37, 179
Fsp4HI GCNGC 3 cut(s) 171, 180, 470
FspBI CTAG 1 cut(s) 1197
GluI GCNGC 3 cut(s) 171, 180, 470
GsaI CCCAGC 1 cut(s) 1113
GsuI CTGGAG 1 cut(s) 1111
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 72, 240, 372
Hin1II CATG 3 cut(s) 110, 1046, 1052
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HindIII AAGCTT 2 cut(s) 348, 782
HpaII CCGG 3 cut(s) 72, 240, 372
HphI GGTGA 5 cut(s) 3, 95, 361, 1069, 1200
Hpy166II GTNNAC 2 cut(s) 24, 1116
Hpy188I TCNGA 9 cut(s) 19, 31, 307, 682, 881, 915, 957, 966, 1005
Hpy188III TCNNGA 6 cut(s) 133, 164, 593, 1049, 1090, 1121
Hpy8I GTNNAC 2 cut(s) 24, 1116
HpyAV CCTTC 5 cut(s) 210, 575, 753, 803, 1000
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 1 cut(s) 1132
HpyCH4V TGCA 5 cut(s) 170, 179, 469, 668, 899
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 179, 781
HpyF3I CTNAG 7 cut(s) 38, 57, 607, 653, 738, 912, 1161
HpySE526I ACGT 1 cut(s) 1132
Hsp92II CATG 3 cut(s) 110, 1046, 1052
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
Ksp22I TGATCA 1 cut(s) 1045
Kzo9I GATC 6 cut(s) 123, 135, 207, 364, 883, 1045
LmnI GCTCC 2 cut(s) 194, 1228
Lsp1109I GCAGC 3 cut(s) 182, 191, 481
LweI GCATC 2 cut(s) 157, 948
MaeI CTAG 1 cut(s) 1197
MaeII ACGT 1 cut(s) 1132
MaeIII GTNAC 4 cut(s) 9, 101, 225, 358
MalI GATC 6 cut(s) 125, 137, 209, 366, 885, 1047
MbiI CCGCTC 3 cut(s) 65, 189, 264
MboI GATC 6 cut(s) 123, 135, 207, 364, 883, 1045
MflI RGATCY 2 cut(s) 123, 364
MluCI AATT 8 cut(s) 267, 290, 547, 596, 639, 856, 1029, 1154
MlyI GAGTC 7 cut(s) 17, 26, 29, 326, 683, 706, 711
MmeI TCCRAC 4 cut(s) 54, 598, 842, 1115
MreI CGCCGGCG 1 cut(s) 239
MroNI GCCGGC 1 cut(s) 239
MroXI GAANNNNTTC 1 cut(s) 813
MseI TTAA 2 cut(s) 489, 564
MslI CAYNNNNRTG 2 cut(s) 800, 1047
MspA1I CMGCKG 1 cut(s) 737
MspI CCGG 3 cut(s) 72, 240, 372
MspR9I CCNGG 2 cut(s) 73, 1173
MvaI CCWGG 1 cut(s) 1173
MwoI GCNNNNNNNGC 3 cut(s) 176, 179, 781
NaeI GCCGGC 1 cut(s) 241
NciI CCSGG 1 cut(s) 73
NdeII GATC 6 cut(s) 123, 135, 207, 364, 883, 1045
NgoMIV GCCGGC 1 cut(s) 239
NlaIII CATG 3 cut(s) 110, 1046, 1052
NlaIV GGNNCC 2 cut(s) 825, 1224
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 4 cut(s) 9, 101, 225, 358
NspV TTCGAA 1 cut(s) 1188
OliI CACNNNNGTG 1 cut(s) 800
PagI TCATGA 1 cut(s) 1048
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PdiI GCCGGC 1 cut(s) 241
PdmI GAANNNNTTC 1 cut(s) 813
PfeI GAWTC 4 cut(s) 512, 850, 1035, 1190
PfoI TCCNGGA 1 cut(s) 71
PkrI GCNGC 3 cut(s) 172, 181, 471
PleI GAGTC 7 cut(s) 16, 26, 28, 325, 682, 706, 711
PpsI GAGTC 7 cut(s) 16, 26, 28, 325, 682, 706, 711
PpuMI RGGWCCY 1 cut(s) 824
Psp1406I AACGTT 1 cut(s) 1132
Psp5II RGGWCCY 1 cut(s) 824
Psp6I CCWGG 1 cut(s) 1171
PspFI CCCAGC 1 cut(s) 1109
PspGI CCWGG 1 cut(s) 1171
PspN4I GGNNCC 2 cut(s) 825, 1224
PspPI GGNCC 3 cut(s) 689, 824, 1009
PspPPI RGGWCCY 1 cut(s) 824
PsuI RGATCY 2 cut(s) 123, 364
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 3 cut(s) 938, 995, 1115
RsaNI GTAC 3 cut(s) 937, 994, 1114
RseI CAYNNNNRTG 2 cut(s) 800, 1047
SaqAI TTAA 2 cut(s) 489, 564
SatI GCNGC 3 cut(s) 171, 180, 470
Sau3AI GATC 6 cut(s) 123, 135, 207, 364, 883, 1045
Sau96I GGNCC 3 cut(s) 689, 824, 1009
SchI GAGTC 7 cut(s) 17, 26, 29, 326, 683, 706, 711
ScrFI CCNGG 2 cut(s) 73, 1173
SfaNI GCATC 2 cut(s) 157, 948
SfuI TTCGAA 1 cut(s) 1188
SgrAI CRCCGGYG 1 cut(s) 239
SinI GGWCC 3 cut(s) 689, 824, 1009
SmiMI CAYNNNNRTG 2 cut(s) 800, 1047
Sse9I AATT 8 cut(s) 267, 290, 547, 596, 639, 856, 1029, 1154
SsiI CCGC 6 cut(s) 63, 187, 237, 243, 262, 687
SspI AATATT 1 cut(s) 696
SspMI CTAG 1 cut(s) 1197
StyD4I CCNGG 2 cut(s) 71, 1171
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 1 cut(s) 1135
TaqI TCGA 7 cut(s) 132, 165, 312, 331, 715, 834, 1188
TasI AATT 8 cut(s) 267, 290, 547, 596, 639, 856, 1029, 1154
TfiI GAWTC 4 cut(s) 512, 850, 1035, 1190
Tru1I TTAA 2 cut(s) 489, 564
Tru9I TTAA 2 cut(s) 489, 564
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 4 cut(s) 9, 101, 225, 358
TseI GCWGC 3 cut(s) 170, 179, 469
Tsp45I GTSAC 4 cut(s) 9, 101, 225, 358
TspDTI ATGAA 5 cut(s) 458, 534, 957, 1065, 1113
TspGWI ACGGA 5 cut(s) 193, 200, 213, 244, 710
TspRI CASTG 1 cut(s) 148
VpaK11BI GGWCC 3 cut(s) 689, 824, 1009
XapI RAATTY 1 cut(s) 547
XmnI GAANNNNTTC 1 cut(s) 813
XspI CTAG 1 cut(s) 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.