MD11G1130200.v1.1

fatty acid beta-oxidation, unsaturated, even number

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002756
Physical Location & Seq
Reverse (-)
11816583 .. 11818948
2366 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1130200.v1.1.491

Sequence Viewer

Length: 798 bp
ATGCTCAGCCGGAACTTACTCTCAGTCAACCTACCTTATTTGAGAGGTTTTTTGTCAGCACCAGCTAGTTTTCTTGGAACTGGAGATACATTAAAGCAAGCTAGAGTATTCACGAATGAAGATGTCATAGAGTGCTCAAAAGTGAGACGTGACTATAATCATCTGCATTTGAATTCTGAGTCTGCTCGAAATGCTGGATTTGAAGATCGACTGGTACACAGGATCCTTGTTACTTCCCCGGTCCCAAATATCACATCTTCTCATTTTGGCTATGCAAAGAGGAGGCTATCGGGTTGCTCCGAACCCGATTTTGAGGATTCGGGTGGTTTGAAGAGTCCTCAGAAGAAGACTCAGGTGGCTGAATCGTTTTTGCACCGGTATAGGAAGAAGGGCTTGCTTGTCACCGACATTACTGGCAGTGAATGGTGTGAAAAACAAATGGAGTTTGTTCTCCTTGTTGGCAAGCGAAAAGAGAGTATGGCAATGAAAGCGGGTATTGCTCGCCATGCAAAACTTGAAGAAGAGGTTGTAAAAAAAGCTAAAGTTCGTGTCAAATTGATTGAAGATAAATGGGATCCGAAGAGCAGGTTATCAGTTTTGTCCGAACCCAATATCGAGGATGCTGTAGGTTTGAAGAGTGCCCAGAAGATGACTCGGGTGGCGGACTCATTGCACCTGTGTAGGAAGAAAGGGTTGAGTGTCACGTACTTTAGTATCATCGACATTGAAACCGTGGTATATGGTTGTTTTTTCGAGATAGTATTTGCAATGTGCCGGTGTAGTAGTAACTATGAATAA

Protein Analysis

266

Amino Acids

29.94

Weight (kDa)

9.25

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 124 - 192 5.6e-14 Exonuclease V - a 5' deoxyribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 576
AciI CCGC 2 cut(s) 491, 662
AclWI GGATC 4 cut(s) 217, 230, 569, 582
AcsI RAATTY 1 cut(s) 172
AfaI GTAC 2 cut(s) 216, 707
AgeI ACCGGT 1 cut(s) 375
AgsI TTSAA 7 cut(s) 172, 203, 331, 518, 563, 634, 728
AjiI CACGTC 1 cut(s) 149
AluBI AGCT 3 cut(s) 65, 101, 539
AluI AGCT 3 cut(s) 65, 101, 539
Alw21I GWGCWC 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 4 cut(s) 217, 230, 569, 582
Ama87I CYCGRG 1 cut(s) 654
ApoI RAATTY 1 cut(s) 172
AsiGI ACCGGT 1 cut(s) 375
AspS9I GGNCC 1 cut(s) 241
AsuC2I CCSGG 1 cut(s) 239
AsuHPI GGTGA 1 cut(s) 394
AvaI CYCGRG 1 cut(s) 654
AvaII GGWCC 1 cut(s) 241
BaeGI GKGCMC 1 cut(s) 643
BaeI ACNNNNGTAYC 2 cut(s) 697, 730
BamHI GGATCC 2 cut(s) 222, 574
BbsI GAAGAC 1 cut(s) 353
Bbv12I GWGCWC 1 cut(s) 137
BcnI CCSGG 1 cut(s) 239
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 2 cut(s) 66, 102
BfmI CTRYAG 1 cut(s) 624
BfuAI ACCTGC 1 cut(s) 576
BlpI GCTNAGC 1 cut(s) 5
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 1 cut(s) 241
BmeT110I CYCGRG 1 cut(s) 654
BmgBI CACGTC 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 241
BmiI GGNNCC 3 cut(s) 224, 243, 576
BmrFI CCNGG 1 cut(s) 239
BmsI GCATC 1 cut(s) 610
BpiI GAAGAC 1 cut(s) 353
BpmI CTGGAG 1 cut(s) 102
Bpu1102I GCTNAGC 1 cut(s) 5
BpuMI CCSGG 1 cut(s) 239
BsaAI YACGTR 1 cut(s) 705
BsaJI CCNNGG 2 cut(s) 237, 732
BsaWI WCCGGW 1 cut(s) 375
Bse118I RCCGGY 2 cut(s) 375, 774
Bse1I ACTGG 3 cut(s) 85, 216, 418
Bse3DI GCAATG 3 cut(s) 489, 668, 774
BseDI CCNNGG 2 cut(s) 237, 732
BseGI GGATG 1 cut(s) 625
BseMI GCAATG 3 cut(s) 489, 668, 774
BseMII CTCAG 5 cut(s) 19, 36, 168, 353, 365
BseNI ACTGG 3 cut(s) 85, 216, 418
BseRI GAGGAG 1 cut(s) 295
BseSI GKGCMC 1 cut(s) 643
BshTI ACCGGT 1 cut(s) 375
BsiHKAI GWGCWC 1 cut(s) 137
BsiHKCI CYCGRG 1 cut(s) 654
BsiSI CCGG 4 cut(s) 10, 239, 376, 775
BslFI GGGAC 1 cut(s) 227
BsmAI GTCTC 1 cut(s) 139
BsmBI CGTCTC 1 cut(s) 139
BsmFI GGGAC 1 cut(s) 227
BsoBI CYCGRG 1 cut(s) 654
Bsp1286I GDGCHC 2 cut(s) 137, 643
Bsp143I GATC 3 cut(s) 205, 222, 574
Bsp1720I GCTNAGC 1 cut(s) 5
BspACI CCGC 2 cut(s) 491, 662
BspCNI CTCAG 5 cut(s) 18, 35, 169, 352, 364
BspLI GGNNCC 3 cut(s) 224, 243, 576
BspMI ACCTGC 1 cut(s) 576
BspPI GGATC 4 cut(s) 217, 230, 569, 582
BspQI GCTCTTC 1 cut(s) 575
BsrDI GCAATG 3 cut(s) 489, 668, 774
BsrFI RCCGGY 2 cut(s) 375, 774
BsrI ACTGG 3 cut(s) 85, 216, 418
BssAI RCCGGY 2 cut(s) 375, 774
BssECI CCNNGG 2 cut(s) 237, 732
BssMI GATC 3 cut(s) 205, 222, 574
Bst4CI ACNGT 1 cut(s) 733
Bst6I CTCTTC 4 cut(s) 326, 516, 575, 629
BstBAI YACGTR 1 cut(s) 705
BstC8I GCNNGC 4 cut(s) 99, 395, 464, 502
BstDEI CTNAG 5 cut(s) 5, 22, 177, 339, 351
BstDSI CCRYGG 1 cut(s) 732
BstF5I GGATG 1 cut(s) 625
BstKTI GATC 3 cut(s) 208, 225, 577
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 3 cut(s) 205, 222, 574
BstMWI GCNNNNNNNGC 4 cut(s) 191, 488, 497, 506
BstSCI CCNGG 1 cut(s) 237
BstSFI CTRYAG 1 cut(s) 624
BstSLI GKGCMC 1 cut(s) 643
BstV2I GAAGAC 1 cut(s) 353
BstX2I RGATCY 2 cut(s) 222, 574
BstYI RGATCY 2 cut(s) 222, 574
BtgI CCRYGG 1 cut(s) 732
BtrI CACGTC 1 cut(s) 149
BtsCI GGATG 1 cut(s) 625
BtsI GCAGTG 1 cut(s) 424
BtsIMutI CAGTG 1 cut(s) 424
BveI ACCTGC 1 cut(s) 576
Cac8I GCNNGC 4 cut(s) 99, 395, 464, 502
Cfr10I RCCGGY 2 cut(s) 375, 774
Cfr13I GGNCC 1 cut(s) 241
Csp6I GTAC 2 cut(s) 215, 706
CspAI ACCGGT 1 cut(s) 375
CviAII CATG 1 cut(s) 506
CviJI RGCY 8 cut(s) 9, 65, 101, 270, 286, 359, 393, 539
CviKI_1 RGCY 8 cut(s) 9, 65, 101, 270, 286, 359, 393, 539
CviQI GTAC 2 cut(s) 215, 706
DdeI CTNAG 5 cut(s) 5, 22, 177, 339, 351
DpnI GATC 3 cut(s) 207, 224, 576
DpnII GATC 3 cut(s) 205, 222, 574
Eam1104I CTCTTC 4 cut(s) 326, 516, 575, 629
EarI CTCTTC 4 cut(s) 326, 516, 575, 629
EciI GGCGGA 1 cut(s) 677
Eco47I GGWCC 1 cut(s) 241
Eco88I CYCGRG 1 cut(s) 654
EcoRI GAATTC 1 cut(s) 172
Esp3I CGTCTC 1 cut(s) 139
FaeI CATG 1 cut(s) 509
FaiI YATR 9 cut(s) 128, 156, 273, 381, 479, 507, 739, 741, 792
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FaqI GGGAC 1 cut(s) 227
FatI CATG 1 cut(s) 505
FauI CCCGC 1 cut(s) 484
FokI GGATG 1 cut(s) 632
FspBI CTAG 2 cut(s) 66, 102
GsuI CTGGAG 1 cut(s) 102
HapII CCGG 4 cut(s) 10, 239, 376, 775
Hin1II CATG 1 cut(s) 509
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HinfI GANTC 7 cut(s) 179, 317, 334, 349, 362, 652, 665
HpaII CCGG 4 cut(s) 10, 239, 376, 775
HphI GGTGA 1 cut(s) 394
Hpy166II GTNNAC 2 cut(s) 28, 217
Hpy188I TCNGA 5 cut(s) 178, 301, 342, 579, 604
Hpy188III TCNNGA 2 cut(s) 112, 754
Hpy8I GTNNAC 2 cut(s) 28, 217
HpyAV CCTTC 1 cut(s) 382
HpyCH4III ACNGT 1 cut(s) 733
HpyCH4IV ACGT 2 cut(s) 148, 704
HpyCH4V TGCA 6 cut(s) 166, 275, 373, 509, 673, 767
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 488, 497, 506
HpyF3I CTNAG 5 cut(s) 5, 22, 177, 339, 351
HpySE526I ACGT 2 cut(s) 148, 704
Hsp92II CATG 1 cut(s) 509
Kzo9I GATC 3 cut(s) 205, 222, 574
LguI GCTCTTC 1 cut(s) 575
LmnI GCTCC 1 cut(s) 302
LweI GCATC 1 cut(s) 610
MaeI CTAG 2 cut(s) 66, 102
MaeII ACGT 2 cut(s) 148, 704
MaeIII GTNAC 5 cut(s) 149, 229, 400, 700, 785
MalI GATC 3 cut(s) 207, 224, 576
MboI GATC 3 cut(s) 205, 222, 574
MflI RGATCY 2 cut(s) 222, 574
MhlI GDGCHC 2 cut(s) 137, 643
MluCI AATT 2 cut(s) 172, 554
MlyI GAGTC 5 cut(s) 188, 343, 343, 646, 659
MnlI CCTC 7 cut(s) 38, 273, 276, 307, 348, 517, 610
MseI TTAA 1 cut(s) 92
MspI CCGG 4 cut(s) 10, 239, 376, 775
MspR9I CCNGG 1 cut(s) 239
MwoI GCNNNNNNNGC 4 cut(s) 191, 488, 497, 506
NciI CCSGG 1 cut(s) 239
NdeII GATC 3 cut(s) 205, 222, 574
NlaIII CATG 1 cut(s) 509
NlaIV GGNNCC 3 cut(s) 224, 243, 576
NmuCI GTSAC 3 cut(s) 149, 400, 700
PciSI GCTCTTC 1 cut(s) 575
PfeI GAWTC 2 cut(s) 317, 362
PinAI ACCGGT 1 cut(s) 375
PleI GAGTC 5 cut(s) 187, 342, 343, 646, 659
PpsI GAGTC 5 cut(s) 187, 342, 343, 646, 659
Ppu21I YACGTR 1 cut(s) 705
PspN4I GGNNCC 3 cut(s) 224, 243, 576
PspPI GGNCC 1 cut(s) 241
PsrI GAACNNNNNNTAC 2 cut(s) 70, 102
PsuI RGATCY 2 cut(s) 222, 574
RsaI GTAC 2 cut(s) 216, 707
RsaNI GTAC 2 cut(s) 215, 706
SapI GCTCTTC 1 cut(s) 575
SaqAI TTAA 1 cut(s) 92
Sau3AI GATC 3 cut(s) 205, 222, 574
Sau96I GGNCC 1 cut(s) 241
SchI GAGTC 5 cut(s) 188, 343, 343, 646, 659
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 2 cut(s) 137, 643
SfaNI GCATC 1 cut(s) 610
SfcI CTRYAG 1 cut(s) 624
SinI GGWCC 1 cut(s) 241
Sse9I AATT 2 cut(s) 172, 554
SsiI CCGC 2 cut(s) 491, 662
SspMI CTAG 2 cut(s) 66, 102
StyD4I CCNGG 1 cut(s) 237
TaaI ACNGT 1 cut(s) 733
TaiI ACGT 2 cut(s) 151, 707
TaqI TCGA 5 cut(s) 187, 208, 615, 720, 753
TasI AATT 2 cut(s) 172, 554
TfiI GAWTC 2 cut(s) 317, 362
Tru1I TTAA 1 cut(s) 92
Tru9I TTAA 1 cut(s) 92
TscAI CASTG 1 cut(s) 424
TseFI GTSAC 3 cut(s) 149, 400, 700
Tsp45I GTSAC 3 cut(s) 149, 400, 700
TspDTI ATGAA 2 cut(s) 132, 500
TspRI CASTG 1 cut(s) 424
VpaK11BI GGWCC 1 cut(s) 241
XapI RAATTY 1 cut(s) 172
XspI CTAG 2 cut(s) 66, 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.