Rh5BG408000

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002756
Physical Location & Seq
Forward (+)
66179431 .. 66211250
31820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG408000.1

Sequence Viewer

Length: 2142 bp
ATGACGGAGTCGCCTTCCGACTCACGACTCTTGGATCATCATCATCCTCATCTTGTGCCCCAAATCCCAATTGAGATTGTGAGCGACGAAGAGATGGCCTTGCTCTTCGCTCGCTCTCTAATCCCTTCCACAGTGAGAGCATTCCCTTCCAGCGTCAGGTCTCTTCAGTACTCCATCACCCTCCTTTCCAAGAGGAGCCTTTGGGGTTGCTCCCAACCCGGTATAGAGGATTCGGGCTGGTTCAACACTACTACCCAGAAGAAGAAGGCTGAATCGCTTTTGCACCGCTTCAGGAACAAGAAGGGCTTGTCCGCCTCCGATTTTGCTGAATCGGAATGGTGTGAACAACAAATGCGGTACATTCTCCTGAATGGCAACAGAGTAATCAGTAAGGCTATGAAAACTGGTAGGGAGCGCCATGCAAAACTTGAAGAAGAGTTTGTAACAAAATCTAAAGTTGTTGTCAATTCAGACGAAGATAAATGGGCCCTGAGGTTTTTGAAGTTTATTACAGGCATAAATCAATTATATACTGAAGGATTGACTCGAGAGCTGCCTCTCGTAGGTTTTGCAGAAGGTATATGGATGGTGGGAGTGATTGATGAGGTTCAAATGCCTGCCACAGAAACCCAGAGAAATCCATTATTAGTTGACACAAAGACTCGTGTGAAAGATAAACTTCCTTCCGAACCACAACGAAGGAAGGGAAGGTTACAATTGATGTTCTACAAGTATTTGTGGGATAATCTAGAAGCATCTGGATTCCCCGCCGACCAATTCTTTGATCATTTTTCCTTAAATCCTGATTGTGACTTATCTGAAGAAATCAGGGAGGTCGTCCGAGCTAAATCAGGGTATCCAGCAAAGACCCTTGATGACGTGGTGAAATACTATAGAAAATCCTGGATCCTGCTACCCCGTGCTCATGACCAACTTTTATTGAGATACGAGTTACAAAAGCATTATTCAGTAATCGGTGAAGATATATTTGCATACGACTCTGATGTGTTTAAGAAGCAACTTCAGGTTTGTCTCGAGTTCTGGCTAGGAAAGCGAGAAGCCAGTTACACACCTGAAGAAGAGCGATGGAAATGCTACAGCTTTCTTCCCTCACCCTTACCACCTCCTCTGCCTCCCGCCAAAATCAAGTCAATCTCCGTCTCCGAGGTCATACTTCCTCAGCTCTCCAGCCTCTTGATTGTCAAATCCGCCTCCGCCAGTGTGCCATCGGAGGCGGAGCTCCAGAAGTTCGGCGTCTCCGACGAACTCAGATACTTCGTCAAGGGACTCACTTCCGAGTTTTTGATTCGGATCTTCAAATGCGCACCGTTTCGATCCAATAGTTTGATTCTATATCTGCAACCGGAGAGCGTCTCTCTCGCCATCGACGAACCAGCTCACAAGCAATTTGAAAGTGCAGAGAACACTTGTGCAACCTCTGGAGAGGGTCTATATGAAGGCCTAGATTGGTTCTCCAACAACACTGCTAACAAGGATGCACCAGAGTATGCACCTGGTACACAGAAGTCGAATGTGTCAGGGGAAGTTATTAGGTGGAATAATGTTTCTGGCCCTGAGAAAATAGATGCTAAGAAGTACATAGAGTTACTTGAAGCAGAGATTGAGGAATTGAATAATGAAGTTGGTAGGAAATCTGCAAATGGACAAAATGAATTGTTGGAGTATCTCAAGTCTCTCGAGCCCCAAAATTTAAAGGAGTTGACTAGTACTGCTGGAGATGATGTTGTGGTTGCAATGGATACATTCATCAAGCGGCTTTTGGCTGTTTCAGATCCTAGCCAAATGAAGGCTAGTGTAACATCAACAACTGCAGCAGAACTTGCAAAGCTGCTTTATTGGCTAATGGTAGTCGGATATAGTCTTCGCAACATTGAAGTTCGTTTTGATATGGAACGAGTACTTGGGAATCCTCCAAAGCTCGCAGAATTGCCTCCAGACTGTGAGGAAGGATCAGATTGTTCGTGCTCTATCGAAATTGAAGCCCACCAGGTGTTCGATATAATGCCTCTTACAACTGACTTATGTGGAAACAAATGCACGTGTGATGCTGAACCATTCTTTGAACTGGTAAGGGGAAAAGTTGGACTTCTTTCACTCATCTTTGTGGAGTTGTACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

713

Amino Acids

80.81

Weight (kDa)

5.27

Isoelectric Point (pI)

51.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 92 - 208 2.3e-30 Exonuclease V - a 5' deoxyribonuclease
Exo5 PF09810 209 - 282 1.6e-08 Exonuclease V - a 5' deoxyribonuclease
Exo5 PF09810 309 - 366 4.2e-06 Exonuclease V - a 5' deoxyribonuclease
DUF760 PF05542 558 - 641 9e-20 Protein of unknown function (DUF760)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1324
AciI CCGC 9 cut(s) 286, 312, 355, 768, 1137, 1209, 1215, 1235, 1774
AclWI GGATC 7 cut(s) 42, 901, 914, 1319, 1329, 1787, 1978
AcsI RAATTY 1 cut(s) 1708
AcuI CTGAAG 6 cut(s) 149, 274, 555, 840, 1007, 1095
AcvI CACGTG 1 cut(s) 2061
AcyI GRCGYC 1 cut(s) 1254
AdeI CACNNNGTG 1 cut(s) 2011
AfaI GTAC 7 cut(s) 170, 359, 1519, 1598, 1729, 1920, 2135
AfiI CCNNNNNNNGG 3 cut(s) 156, 563, 1807
AflIII ACRYGT 1 cut(s) 2060
AhlI ACTAGT 1 cut(s) 1724
AjiI CACGTC 1 cut(s) 880
AjnI CCWGG 3 cut(s) 902, 1513, 2007
AjuI GAANNNNNNNTTGG 2 cut(s) 1905, 1937
AluBI AGCT 8 cut(s) 553, 845, 1101, 1183, 1240, 1397, 1849, 1939
AluI AGCT 8 cut(s) 553, 845, 1101, 1183, 1240, 1397, 1849, 1939
Alw21I GWGCWC 3 cut(s) 925, 1242, 1988
Alw26I GTCTC 6 cut(s) 165, 1037, 1165, 1261, 1378, 1698
AlwI GGATC 7 cut(s) 42, 901, 914, 1319, 1329, 1787, 1978
Ama87I CYCGRG 3 cut(s) 546, 1034, 1697
AoxI GGCC 4 cut(s) 96, 486, 1459, 1570
ApaI GGGCCC 1 cut(s) 490
ApeKI GCWGC 3 cut(s) 553, 1832, 1849
ApoI RAATTY 1 cut(s) 1708
ArsI GACNNNNNNTTYG 2 cut(s) 764, 796
AspLEI GCGC 2 cut(s) 417, 1325
AspS9I GGNCC 3 cut(s) 486, 487, 1571
AsuC2I CCSGG 1 cut(s) 219
AsuHPI GGTGA 4 cut(s) 169, 895, 989, 1104
AvaI CYCGRG 3 cut(s) 546, 1034, 1697
AxyI CCTNAGG 1 cut(s) 491
BaeGI GKGCMC 2 cut(s) 60, 490
BamHI GGATCC 1 cut(s) 906
BanII GRGCYC 3 cut(s) 490, 1242, 1704
BauI CACGAG 1 cut(s) 663
BbrPI CACGTG 1 cut(s) 2061
BbsI GAAGAC 1 cut(s) 1874
Bbv12I GWGCWC 3 cut(s) 925, 1242, 1988
BbvCI CCTCAGC 1 cut(s) 1179
BbvI GCAGC 3 cut(s) 540, 1836, 1844
BccI CCATC 6 cut(s) 88, 182, 580, 1080, 1234, 1391
BcgI CGANNNNNNTGC 2 cut(s) 1074, 1108
BciT130I CCWGG 3 cut(s) 904, 1515, 2009
BciVI GTATCC 2 cut(s) 867, 1753
BclI TGATCA 1 cut(s) 784
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 6 cut(s) 165, 1037, 1165, 1261, 1378, 1698
BcuI ACTAGT 1 cut(s) 1724
BfaI CTAG 6 cut(s) 749, 1046, 1463, 1725, 1797, 1812
BfmI CTRYAG 3 cut(s) 892, 1096, 1830
BfoI RGCGCY 1 cut(s) 418
BfuI GTATCC 2 cut(s) 867, 1753
BisI GCNGC 4 cut(s) 554, 1775, 1833, 1850
BlsI GCNGC 4 cut(s) 555, 1776, 1834, 1851
BmcAI AGTACT 3 cut(s) 170, 1729, 1920
Bme1390I CCNGG 4 cut(s) 219, 904, 1515, 2009
BmeT110I CYCGRG 3 cut(s) 546, 1034, 1697
BmgBI CACGTC 1 cut(s) 880
BmgT120I GGNCC 3 cut(s) 486, 487, 1571
BmiI GGNNCC 3 cut(s) 197, 488, 908
BmrFI CCNGG 4 cut(s) 219, 904, 1515, 2009
BmsI GCATC 4 cut(s) 764, 1486, 1576, 2056
BpiI GAAGAC 1 cut(s) 1874
BplI GAGNNNNNCTC 4 cut(s) 1358, 1390, 1360, 1392
BpmI CTGGAG 5 cut(s) 1171, 1226, 1461, 1755, 1938
Bpu10I CCTNAGC 1 cut(s) 1179
BpuEI CTTGAG 1 cut(s) 1673
BpuMI CCSGG 1 cut(s) 219
BsaAI YACGTR 1 cut(s) 2061
BsaBI GATNNNNATC 2 cut(s) 39, 1310
BsaHI GRCGYC 1 cut(s) 1254
BsaI GGTCTC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 1164
BsaWI WCCGGW 1 cut(s) 1363
BsaXI ACNNNNNCTCC 2 cut(s) 1728, 1758
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 563, 1807
Bse1I ACTGG 4 cut(s) 409, 1062, 1218, 2091
Bse21I CCTNAGG 1 cut(s) 491
Bse3DI GCAATG 1 cut(s) 1761
Bse8I GATNNNNATC 2 cut(s) 39, 1310
BseBI CCWGG 3 cut(s) 904, 1515, 2009
BseDI CCNNGG 1 cut(s) 1164
BseGI GGATG 3 cut(s) 43, 591, 1501
BseJI GATNNNNATC 2 cut(s) 39, 1310
BseLI CCNNNNNNNGG 3 cut(s) 156, 563, 1807
BseMI GCAATG 1 cut(s) 1761
BseMII CTCAG 4 cut(s) 482, 1193, 1282, 1566
BseNI ACTGG 4 cut(s) 409, 1062, 1218, 2091
BseRI GAGGAG 2 cut(s) 208, 1116
BseSI GKGCMC 2 cut(s) 60, 490
BseXI GCAGC 3 cut(s) 540, 1836, 1844
BsgI GTGCAG 1 cut(s) 1437
BshFI GGCC 4 cut(s) 98, 488, 1461, 1572
BsiHKAI GWGCWC 3 cut(s) 925, 1242, 1988
BsiHKCI CYCGRG 3 cut(s) 546, 1034, 1697
BsiSI CCGG 2 cut(s) 219, 1364
BslFI GGGAC 1 cut(s) 1299
BslI CCNNNNNNNGG 3 cut(s) 156, 563, 1807
BsmAI GTCTC 6 cut(s) 165, 1037, 1165, 1261, 1378, 1698
BsmBI CGTCTC 3 cut(s) 1165, 1261, 1378
BsmFI GGGAC 1 cut(s) 1299
BsmI GAATGC 1 cut(s) 140
BsnI GGCC 4 cut(s) 98, 488, 1461, 1572
Bso31I GGTCTC 1 cut(s) 165
BsoBI CYCGRG 3 cut(s) 546, 1034, 1697
Bsp120I GGGCCC 1 cut(s) 486
Bsp1286I GDGCHC 6 cut(s) 60, 490, 925, 1242, 1704, 1988
Bsp143I GATC 7 cut(s) 34, 784, 906, 1311, 1334, 1792, 1970
BspACI CCGC 9 cut(s) 286, 312, 355, 768, 1137, 1209, 1215, 1235, 1774
BspANI GGCC 4 cut(s) 98, 488, 1461, 1572
BspCNI CTCAG 4 cut(s) 483, 1192, 1281, 1567
BspHI TCATGA 1 cut(s) 925
BspLI GGNNCC 3 cut(s) 197, 488, 908
BspMAI CTGCAG 1 cut(s) 1834
BspPI GGATC 7 cut(s) 42, 901, 914, 1319, 1329, 1787, 1978
BspQI GCTCTTC 2 cut(s) 110, 1074
BspTNI GGTCTC 1 cut(s) 165
BsrDI GCAATG 1 cut(s) 1761
BsrI ACTGG 4 cut(s) 409, 1062, 1218, 2091
BssECI CCNNGG 1 cut(s) 1164
BssMI GATC 7 cut(s) 34, 784, 906, 1311, 1334, 1792, 1970
BssNI GRCGYC 1 cut(s) 1254
BssSI CACGAG 1 cut(s) 663
Bst2BI CACGAG 1 cut(s) 663
Bst2UI CCWGG 3 cut(s) 904, 1515, 2009
Bst4CI ACNGT 3 cut(s) 133, 1329, 1961
Bst6I CTCTTC 5 cut(s) 84, 110, 168, 429, 1074
BstACI GRCGYC 1 cut(s) 1254
BstAPI GCANNNNNTGC 1 cut(s) 1841
BstBAI YACGTR 1 cut(s) 2061
BstC8I GCNNGC 3 cut(s) 112, 618, 1941
BstDEI CTNAG 5 cut(s) 491, 1179, 1268, 1575, 1590
BstENI CCTNNNNNAGG 1 cut(s) 561
BstF5I GGATG 3 cut(s) 43, 591, 1501
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 2 cut(s) 417, 1325
BstKTI GATC 7 cut(s) 37, 787, 909, 1314, 1337, 1795, 1973
BstMAI GTCTC 6 cut(s) 165, 1037, 1165, 1261, 1378, 1698
BstMBI GATC 7 cut(s) 34, 784, 906, 1311, 1334, 1792, 1970
BstMWI GCNNNNNNNGC 3 cut(s) 1051, 1841, 1858
BstNI CCWGG 3 cut(s) 904, 1515, 2009
BstSCI CCNGG 4 cut(s) 217, 902, 1513, 2007
BstSFI CTRYAG 3 cut(s) 892, 1096, 1830
BstSLI GKGCMC 2 cut(s) 60, 490
BstV1I GCAGC 3 cut(s) 540, 1836, 1844
BstV2I GAAGAC 1 cut(s) 1874
BstX2I RGATCY 3 cut(s) 906, 1311, 1792
BstYI RGATCY 3 cut(s) 906, 1311, 1792
Bsu36I CCTNAGG 1 cut(s) 491
BsuI GTATCC 2 cut(s) 867, 1753
BsuRI GGCC 4 cut(s) 98, 488, 1461, 1572
BtgZI GCGATG 1 cut(s) 1099
BtrI CACGTC 1 cut(s) 880
BtsCI GGATG 3 cut(s) 43, 591, 1501
BtsI GCAGTG 1 cut(s) 1482
BtsIMutI CAGTG 3 cut(s) 138, 1225, 1482
Cac8I GCNNGC 3 cut(s) 112, 618, 1941
CciI TCATGA 1 cut(s) 925
CfoI GCGC 2 cut(s) 417, 1325
Cfr13I GGNCC 3 cut(s) 486, 487, 1571
CseI GACGC 3 cut(s) 142, 1243, 1360
CsiI ACCWGGT 2 cut(s) 1513, 2007
Csp6I GTAC 7 cut(s) 169, 358, 1518, 1597, 1728, 1919, 2134
CviAII CATG 2 cut(s) 419, 926
CviQI GTAC 7 cut(s) 169, 358, 1518, 1597, 1728, 1919, 2134
DdeI CTNAG 5 cut(s) 491, 1179, 1268, 1575, 1590
DpnI GATC 7 cut(s) 36, 786, 908, 1313, 1336, 1794, 1972
DpnII GATC 7 cut(s) 34, 784, 906, 1311, 1334, 1792, 1970
DraI TTTAAA 1 cut(s) 1713
DraIII CACNNNGTG 1 cut(s) 2011
Eam1104I CTCTTC 5 cut(s) 84, 110, 168, 429, 1074
EarI CTCTTC 5 cut(s) 84, 110, 168, 429, 1074
EciI GGCGGA 4 cut(s) 301, 1198, 1204, 1250
Ecl136II GAGCTC 1 cut(s) 1240
Eco147I AGGCCT 1 cut(s) 1461
Eco24I GRGCYC 3 cut(s) 490, 1242, 1704
Eco31I GGTCTC 1 cut(s) 165
Eco53kI GAGCTC 1 cut(s) 1240
Eco57I CTGAAG 6 cut(s) 149, 274, 555, 840, 1007, 1095
Eco72I CACGTG 1 cut(s) 2061
Eco81I CCTNAGG 1 cut(s) 491
Eco88I CYCGRG 3 cut(s) 546, 1034, 1697
EcoICRI GAGCTC 1 cut(s) 1240
EcoNI CCTNNNNNAGG 1 cut(s) 561
EcoO109I RGGNCCY 1 cut(s) 487
EcoRII CCWGG 3 cut(s) 902, 1513, 2007
EcoT38I GRGCYC 3 cut(s) 490, 1242, 1704
Esp3I CGTCTC 3 cut(s) 1165, 1261, 1378
FaeI CATG 2 cut(s) 422, 929
FalI AAGNNNNNCTT 6 cut(s) 290, 322, 663, 695, 2091, 2123
FaqI GGGAC 1 cut(s) 1299
FatI CATG 2 cut(s) 418, 925
FauI CCCGC 2 cut(s) 775, 1144
FbaI TGATCA 1 cut(s) 784
Fnu4HI GCNGC 4 cut(s) 554, 1775, 1833, 1850
FokI GGATG 3 cut(s) 30, 598, 1508
FriOI GRGCYC 3 cut(s) 490, 1242, 1704
Fsp4HI GCNGC 4 cut(s) 554, 1775, 1833, 1850
FspAI RTGCGCAY 1 cut(s) 1324
FspBI CTAG 6 cut(s) 749, 1046, 1463, 1725, 1797, 1812
FspI TGCGCA 1 cut(s) 1324
GlaI GCGC 2 cut(s) 416, 1324
GluI GCNGC 4 cut(s) 554, 1775, 1833, 1850
GsuI CTGGAG 5 cut(s) 1171, 1226, 1461, 1755, 1938
HaeII RGCGCY 1 cut(s) 418
HaeIII GGCC 4 cut(s) 98, 488, 1461, 1572
HapII CCGG 2 cut(s) 219, 1364
HgaI GACGC 3 cut(s) 142, 1243, 1360
HhaI GCGC 2 cut(s) 417, 1325
Hin1I GRCGYC 1 cut(s) 1254
Hin1II CATG 2 cut(s) 422, 929
Hin6I GCGC 2 cut(s) 415, 1323
HinP1I GCGC 2 cut(s) 415, 1323
HincII GTYRAC 2 cut(s) 652, 1722
HindII GTYRAC 2 cut(s) 652, 1722
HpaII CCGG 2 cut(s) 219, 1364
HphI GGTGA 4 cut(s) 169, 895, 989, 1104
Hpy166II GTNNAC 4 cut(s) 344, 652, 1520, 1722
Hpy8I GTNNAC 4 cut(s) 344, 652, 1520, 1722
Hpy99I CGWCG 3 cut(s) 89, 1265, 1391
HpyCH4III ACNGT 3 cut(s) 133, 1329, 1961
HpyCH4IV ACGT 2 cut(s) 879, 2060
HpyF10VI GCNNNNNNNGC 3 cut(s) 1051, 1841, 1858
HpyF3I CTNAG 5 cut(s) 491, 1179, 1268, 1575, 1590
HpySE526I ACGT 2 cut(s) 879, 2060
Hsp92I GRCGYC 1 cut(s) 1254
Hsp92II CATG 2 cut(s) 422, 929
HspAI GCGC 2 cut(s) 415, 1323
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 7 cut(s) 34, 784, 906, 1311, 1334, 1792, 1970
LguI GCTCTTC 2 cut(s) 110, 1074
LmnI GCTCC 5 cut(s) 195, 215, 412, 1237, 1245
Lsp1109I GCAGC 3 cut(s) 540, 1836, 1844
LweI GCATC 4 cut(s) 764, 1486, 1576, 2056
MabI ACCWGGT 2 cut(s) 1513, 2007
MaeI CTAG 6 cut(s) 749, 1046, 1463, 1725, 1797, 1812
MaeII ACGT 2 cut(s) 879, 2060
MaeIII GTNAC 7 cut(s) 442, 711, 809, 951, 1064, 1605, 1816
MalI GATC 7 cut(s) 36, 786, 908, 1313, 1336, 1794, 1972
MboI GATC 7 cut(s) 34, 784, 906, 1311, 1334, 1792, 1970
MfeI CAATTG 2 cut(s) 69, 716
MflI RGATCY 3 cut(s) 906, 1311, 1792
MhlI GDGCHC 6 cut(s) 60, 490, 925, 1242, 1704, 1988
MlyI GAGTC 7 cut(s) 14, 17, 21, 538, 655, 992, 1281
MmeI TCCRAC 6 cut(s) 42, 1284, 1500, 1659, 1852, 2083
MseI TTAA 3 cut(s) 797, 1011, 1712
MslI CAYNNNNRTG 1 cut(s) 2123
MspI CCGG 2 cut(s) 219, 1364
MspR9I CCNGG 4 cut(s) 219, 904, 1515, 2009
MunI CAATTG 2 cut(s) 69, 716
Mva1269I GAATGC 1 cut(s) 140
MvaI CCWGG 3 cut(s) 904, 1515, 2009
MwoI GCNNNNNNNGC 3 cut(s) 1051, 1841, 1858
NciI CCSGG 1 cut(s) 219
NdeII GATC 7 cut(s) 34, 784, 906, 1311, 1334, 1792, 1970
NlaIII CATG 2 cut(s) 422, 929
NlaIV GGNNCC 3 cut(s) 197, 488, 908
NmuCI GTSAC 1 cut(s) 809
NsbI TGCGCA 1 cut(s) 1324
PaeR7I CTCGAG 3 cut(s) 546, 1034, 1697
PagI TCATGA 1 cut(s) 925
PceI AGGCCT 1 cut(s) 1461
PciSI GCTCTTC 2 cut(s) 110, 1074
PcsI WCGNNNNNNNCGW 2 cut(s) 1257, 1386
PctI GAATGC 1 cut(s) 140
PfeI GAWTC 7 cut(s) 230, 272, 329, 762, 1306, 1348, 1927
PflFI GACNNNGTC 1 cut(s) 7
PfoI TCCNGGA 1 cut(s) 902
PkrI GCNGC 4 cut(s) 555, 1776, 1834, 1851
PleI GAGTC 7 cut(s) 14, 16, 21, 538, 655, 992, 1281
PmaCI CACGTG 1 cut(s) 2061
PmlI CACGTG 1 cut(s) 2061
PpsI GAGTC 7 cut(s) 14, 16, 21, 538, 655, 992, 1281
Ppu21I YACGTR 1 cut(s) 2061
Psp124BI GAGCTC 1 cut(s) 1242
Psp6I CCWGG 3 cut(s) 902, 1513, 2007
PspCI CACGTG 1 cut(s) 2061
PspGI CCWGG 3 cut(s) 902, 1513, 2007
PspN4I GGNNCC 3 cut(s) 197, 488, 908
PspOMI GGGCCC 1 cut(s) 486
PspPI GGNCC 3 cut(s) 486, 487, 1571
PstI CTGCAG 1 cut(s) 1834
PsuI RGATCY 3 cut(s) 906, 1311, 1792
PsyI GACNNNGTC 1 cut(s) 7
RsaI GTAC 7 cut(s) 170, 359, 1519, 1598, 1729, 1920, 2135
RsaNI GTAC 7 cut(s) 169, 358, 1518, 1597, 1728, 1919, 2134
RseI CAYNNNNRTG 1 cut(s) 2123
SacI GAGCTC 1 cut(s) 1242
SapI GCTCTTC 2 cut(s) 110, 1074
SaqAI TTAA 3 cut(s) 797, 1011, 1712
SatI GCNGC 4 cut(s) 554, 1775, 1833, 1850
Sau3AI GATC 7 cut(s) 34, 784, 906, 1311, 1334, 1792, 1970
Sau96I GGNCC 3 cut(s) 486, 487, 1571
ScaI AGTACT 3 cut(s) 170, 1729, 1920
SchI GAGTC 7 cut(s) 14, 17, 21, 538, 655, 992, 1281
ScrFI CCNGG 4 cut(s) 219, 904, 1515, 2009
SduI GDGCHC 6 cut(s) 60, 490, 925, 1242, 1704, 1988
SexAI ACCWGGT 2 cut(s) 1513, 2007
SfaNI GCATC 4 cut(s) 764, 1486, 1576, 2056
SfcI CTRYAG 3 cut(s) 892, 1096, 1830
Sfr274I CTCGAG 3 cut(s) 546, 1034, 1697
SlaI CTCGAG 3 cut(s) 546, 1034, 1697
SmiMI CAYNNNNRTG 1 cut(s) 2123
SmlI CTYRAG 4 cut(s) 546, 1034, 1688, 1697
SmoI CTYRAG 4 cut(s) 546, 1034, 1688, 1697
SpeI ACTAGT 1 cut(s) 1724
SseBI AGGCCT 1 cut(s) 1461
SsiI CCGC 9 cut(s) 286, 312, 355, 768, 1137, 1209, 1215, 1235, 1774
SspMI CTAG 6 cut(s) 749, 1046, 1463, 1725, 1797, 1812
SstI GAGCTC 1 cut(s) 1242
StuI AGGCCT 1 cut(s) 1461
StyD4I CCNGG 4 cut(s) 217, 902, 1513, 2007
TaaI ACNGT 3 cut(s) 133, 1329, 1961
TaiI ACGT 2 cut(s) 882, 2063
TaqI TCGA 8 cut(s) 547, 1035, 1333, 1386, 1529, 1698, 1992, 2016
TatI WGTACW 4 cut(s) 168, 1596, 1727, 1918
TauI GCSGC 1 cut(s) 1777
TfiI GAWTC 7 cut(s) 230, 272, 329, 762, 1306, 1348, 1927
Tru1I TTAA 3 cut(s) 797, 1011, 1712
Tru9I TTAA 3 cut(s) 797, 1011, 1712
TscAI CASTG 3 cut(s) 138, 1225, 1489
TseFI GTSAC 1 cut(s) 809
TseI GCWGC 3 cut(s) 553, 1832, 1849
Tsp45I GTSAC 1 cut(s) 809
TspDTI ATGAA 6 cut(s) 413, 1470, 1653, 1686, 1756, 1820
TspGWI ACGGA 2 cut(s) 20, 1147
TspRI CASTG 3 cut(s) 138, 1225, 1489
Tth111I GACNNNGTC 1 cut(s) 7
XagI CCTNNNNNAGG 1 cut(s) 561
XapI RAATTY 1 cut(s) 1708
XbaI TCTAGA 1 cut(s) 748
XhoI CTCGAG 3 cut(s) 546, 1034, 1697
XspI CTAG 6 cut(s) 749, 1046, 1463, 1725, 1797, 1812
ZrmI AGTACT 3 cut(s) 170, 1729, 1920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.