MD03G1203800.v1.1

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002756
Physical Location & Seq
Forward (+)
27847329 .. 27849847
2519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1203800.v1.1.491

Sequence Viewer

Length: 333 bp
ATGGCTGCTAACTCAAGGTTCCCAGTAGAGACCCTTGATGACATAGTGAGATACTACAGAAACATATGTAGCATGCTGCCCTCTACACATGACCAGCTGCTATTGAGATATGAGTTACAGAAAGATCATTCGTTACTTGGTAAAGATGAGTTTGCATACGACTCTGATTGGGTCAAGAATCAAATACAGGGTTGTCTTGAGTTCTGGTTAGGAGAGCGAGAAGCAAGATACACTCCTAAGGATGAGGGATGGAAATGCAGGTTCTGTCAATATTCTTCTGTTTGCCCCGCTAGAAGTGCAGAGTCTGCAAACTATACTAATACACCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

111

Amino Acids

12.87

Weight (kDa)

5.32

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 25 - 95 9.5e-13 Exonuclease V - a 5' deoxyribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 249
AciI CCGC 1 cut(s) 288
AluBI AGCT 1 cut(s) 97
AluI AGCT 1 cut(s) 97
Alw26I GTCTC 1 cut(s) 23
AlwNI CAGNNNCTG 2 cut(s) 264, 305
ApeKI GCWGC 3 cut(s) 5, 76, 97
AxyI CCTNAGG 1 cut(s) 237
BbvI GCAGC 2 cut(s) 63, 84
BccI CCATC 1 cut(s) 243
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 1 cut(s) 291
BfmI CTRYAG 1 cut(s) 55
BfuAI ACCTGC 1 cut(s) 249
BisI GCNGC 3 cut(s) 6, 77, 98
BlsI GCNGC 3 cut(s) 7, 78, 99
BmiI GGNNCC 1 cut(s) 20
BmrI ACTGGG 1 cut(s) 17
BmuI ACTGGG 1 cut(s) 17
BpuEI CTTGAG 1 cut(s) 218
BsaI GGTCTC 1 cut(s) 23
Bse1I ACTGG 1 cut(s) 23
Bse21I CCTNAGG 1 cut(s) 237
BseGI GGATG 2 cut(s) 247, 254
BseNI ACTGG 1 cut(s) 23
BseXI GCAGC 2 cut(s) 63, 84
BsgI GTGCAG 1 cut(s) 318
BsmAI GTCTC 1 cut(s) 23
Bso31I GGTCTC 1 cut(s) 23
Bsp143I GATC 1 cut(s) 124
BspACI CCGC 1 cut(s) 288
BspLI GGNNCC 1 cut(s) 20
BspMI ACCTGC 1 cut(s) 249
BspTNI GGTCTC 1 cut(s) 23
BsrI ACTGG 1 cut(s) 23
BssMI GATC 1 cut(s) 124
BstAPI GCANNNNNTGC 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 74
BstDEI CTNAG 1 cut(s) 237
BstF5I GGATG 2 cut(s) 247, 254
BstKTI GATC 1 cut(s) 127
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 1 cut(s) 124
BstMWI GCNNNNNNNGC 2 cut(s) 296, 305
BstNSI RCATGY 1 cut(s) 76
BstSFI CTRYAG 1 cut(s) 55
BstV1I GCAGC 2 cut(s) 63, 84
Bsu36I CCTNAGG 1 cut(s) 237
BtsCI GGATG 2 cut(s) 247, 254
BveI ACCTGC 1 cut(s) 249
Cac8I GCNNGC 1 cut(s) 74
CaiI CAGNNNCTG 2 cut(s) 264, 305
CviAII CATG 2 cut(s) 73, 89
CviJI RGCY 2 cut(s) 5, 97
CviKI_1 RGCY 2 cut(s) 5, 97
DdeI CTNAG 1 cut(s) 237
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
Eco31I GGTCTC 1 cut(s) 23
Eco81I CCTNAGG 1 cut(s) 237
FaeI CATG 2 cut(s) 76, 92
FaiI YATR 8 cut(s) 44, 65, 67, 74, 90, 111, 157, 315
FatI CATG 2 cut(s) 72, 88
FauI CCCGC 1 cut(s) 295
FauNDI CATATG 1 cut(s) 65
Fnu4HI GCNGC 3 cut(s) 6, 77, 98
FokI GGATG 2 cut(s) 254, 261
Fsp4HI GCNGC 3 cut(s) 6, 77, 98
FspBI CTAG 1 cut(s) 291
GluI GCNGC 3 cut(s) 6, 77, 98
Hin1II CATG 2 cut(s) 76, 92
HinfI GANTC 3 cut(s) 161, 178, 302
Hpy188I TCNGA 1 cut(s) 166
Hpy188III TCNNGA 2 cut(s) 175, 197
HpyCH4V TGCA 4 cut(s) 155, 258, 299, 308
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 305
HpyF3I CTNAG 1 cut(s) 237
Hsp92II CATG 2 cut(s) 76, 92
Kzo9I GATC 1 cut(s) 124
LpnPI CCDG 5 cut(s) 36, 107, 173, 190, 244
Lsp1109I GCAGC 2 cut(s) 63, 84
MaeI CTAG 1 cut(s) 291
MaeIII GTNAC 2 cut(s) 114, 132
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MboII GAAGA 1 cut(s) 267
MlyI GAGTC 2 cut(s) 155, 311
MnlI CCTC 2 cut(s) 91, 238
MspA1I CMGCKG 1 cut(s) 97
MwoI GCNNNNNNNGC 2 cut(s) 296, 305
NdeI CATATG 1 cut(s) 65
NdeII GATC 1 cut(s) 124
NlaIII CATG 2 cut(s) 76, 92
NlaIV GGNNCC 1 cut(s) 20
NspI RCATGY 1 cut(s) 76
PaeI GCATGC 1 cut(s) 76
PfeI GAWTC 1 cut(s) 178
PkrI GCNGC 3 cut(s) 7, 78, 99
PleI GAGTC 2 cut(s) 155, 310
PpsI GAGTC 2 cut(s) 155, 310
PspN4I GGNNCC 1 cut(s) 20
PstNI CAGNNNCTG 2 cut(s) 264, 305
PvuII CAGCTG 1 cut(s) 97
SatI GCNGC 3 cut(s) 6, 77, 98
Sau3AI GATC 1 cut(s) 124
SchI GAGTC 2 cut(s) 155, 311
SetI ASST 3 cut(s) 20, 99, 263
SfcI CTRYAG 1 cut(s) 55
SmlI CTYRAG 2 cut(s) 13, 197
SmoI CTYRAG 2 cut(s) 13, 197
SphI GCATGC 1 cut(s) 76
SsiI CCGC 1 cut(s) 288
SspI AATATT 1 cut(s) 272
SspMI CTAG 1 cut(s) 291
TfiI GAWTC 1 cut(s) 178
TseI GCWGC 3 cut(s) 5, 76, 97
XceI RCATGY 1 cut(s) 76
XspI CTAG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.