RLG00000035376

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002756
Physical Location & Seq
Forward (+)
66028151 .. 66034398
6248 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035376

Sequence Viewer

Length: 1071 bp
ATGGCCTTGCTCTGCGCTCGCTCTCTAATCCCTTCCACAGTGAGAGCATTCCCTTCCAGCGTCAGGTCTCTTCAGTACTCCATCACCCTCCTTTCCAAGAGGAGCCTTTCGGGTTGCTCCCAACCCGGTATAGTGGATTCGGGCGGGTTCAACACTACTACCCAGAAGAAGAAGAAGGCTGAATCGTTTTTGCACCGCTTCAGGAACAAGAAGGGCTTGTCCGCCACCTATTTTGCTGAGTCGGAATGGTGTGAAAAACAAACGCAGTATGTTCTCCTGAATGGCAACAGAGTAATCAGTAAAGCTATGAAAACTGGTAGGGAGCGCCATGCAAAACTTGGAGAAGAATTTGTAACAAAATTTAAAGTTGTTGTCAATTCAGACGAAGATAAATGGGCCCTGAAGTTTTTGAAGTTTATTACAGGCATAAATCGATTATATACTGAAGGATTGACTCGAGAGCTGCCTCTCGTAGGTTTTGCAGAACGTATATGGATGGTGGGAGTGATTGATGAGGTTCAAATGCCTGCCACAGAAACCCAGAGAAATCCACTACTAGTTGACACAAAGACTTGTGTGAAAGATAAACTTCCTTCCGAACCACAACGAAGGAAGGGAAGGTTTCAATTGATGTTCTACAAGTATTTGTGGGATAATCTAGAAGCATCTAGATTCCCCTCCGACCAATTCTTTGATTATTTTTCCTTAAATCCTGATTGTGACTTATCTGATGAAATCAGGGAGGTCCTAGCTAAATCAGGGTATCCAGCAAAGACCCTTGATGACGTAGTGAAATACTATAGAAAATCCTGGATCCTGCTACCCCGTGCTCATGACCAACTTTTATTGAGATACGAGTTACAAAAGGATTACTCAGTAATTGCTGAAAATATATTTGCATACGACTCTGATGGGTTCAACAAGCAATTTCAGGTTTCTCTTGAGTTCTGGCTAGGAAAGCGAGAAGCCAGTTACACACCTGAAGAAGAGCGATGGAAATGCTACTGCTGTGAATTTGCTTCCACATGTCCTGCAGCTCCTGCTAATTTATCATTAGACCATTCACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

357

Amino Acids

41.12

Weight (kDa)

8.88

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 62 - 178 2.8e-27 Exonuclease V - a 5' deoxyribonuclease
Exo5 PF09810 179 - 252 1.8e-07 Exonuclease V - a 5' deoxyribonuclease
Exo5 PF09810 277 - 343 1.2e-10 Exonuclease V - a 5' deoxyribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 144, 196, 222
AclWI GGATC 2 cut(s) 808, 821
AcsI RAATTY 3 cut(s) 347, 359, 1013
AcuI CTGAAG 5 cut(s) 56, 184, 422, 465, 1002
AfaI GTAC 1 cut(s) 77
AfiI CCNNNNNNNGG 2 cut(s) 63, 473
AflIII ACRYGT 1 cut(s) 1025
AgsI TTSAA 5 cut(s) 151, 412, 521, 626, 919
AhlI ACTAGT 1 cut(s) 556
AjnI CCWGG 1 cut(s) 809
AluBI AGCT 4 cut(s) 305, 463, 752, 1037
AluI AGCT 4 cut(s) 305, 463, 752, 1037
Alw21I GWGCWC 1 cut(s) 832
Alw26I GTCTC 1 cut(s) 72
AlwI GGATC 2 cut(s) 808, 821
AlwNI CAGNNNCTG 1 cut(s) 1040
Ama87I CYCGRG 1 cut(s) 456
AoxI GGCC 2 cut(s) 3, 396
ApaI GGGCCC 1 cut(s) 400
ApeKI GCWGC 2 cut(s) 463, 1034
ApoI RAATTY 3 cut(s) 347, 359, 1013
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
AspLEI GCGC 2 cut(s) 17, 327
AspS9I GGNCC 3 cut(s) 396, 397, 745
AsuC2I CCSGG 1 cut(s) 126
AsuHPI GGTGA 1 cut(s) 76
AvaI CYCGRG 1 cut(s) 456
AvaII GGWCC 1 cut(s) 745
BaeGI GKGCMC 1 cut(s) 400
BamHI GGATCC 1 cut(s) 813
BanII GRGCYC 1 cut(s) 400
Bbv12I GWGCWC 1 cut(s) 832
BbvI GCAGC 2 cut(s) 450, 1046
BccI CCATC 4 cut(s) 89, 490, 905, 987
BcgI CGANNNNNNTGC 2 cut(s) 981, 1015
BciT130I CCWGG 1 cut(s) 811
BciVI GTATCC 1 cut(s) 774
BcnI CCSGG 1 cut(s) 126
BcoDI GTCTC 1 cut(s) 72
BcuI ACTAGT 1 cut(s) 556
BfaI CTAG 5 cut(s) 557, 659, 669, 749, 953
BfmI CTRYAG 2 cut(s) 799, 1032
BfoI RGCGCY 1 cut(s) 328
BfuI GTATCC 1 cut(s) 774
BisI GCNGC 2 cut(s) 464, 1035
BlsI GCNGC 2 cut(s) 465, 1036
BmcAI AGTACT 1 cut(s) 77
Bme1390I CCNGG 2 cut(s) 126, 811
Bme18I GGWCC 1 cut(s) 745
BmeT110I CYCGRG 1 cut(s) 456
BmgT120I GGNCC 3 cut(s) 396, 397, 745
BmiI GGNNCC 3 cut(s) 104, 398, 815
BmrFI CCNGG 2 cut(s) 126, 811
BmsI GCATC 1 cut(s) 674
BpuEI CTTGAG 1 cut(s) 962
BpuMI CCSGG 1 cut(s) 126
Bsa29I ATCGAT 1 cut(s) 433
BsaI GGTCTC 1 cut(s) 72
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 473
Bse1I ACTGG 2 cut(s) 319, 969
BseBI CCWGG 1 cut(s) 811
BseCI ATCGAT 1 cut(s) 433
BseGI GGATG 1 cut(s) 501
BseLI CCNNNNNNNGG 2 cut(s) 63, 473
BseMII CTCAG 2 cut(s) 228, 888
BseNI ACTGG 2 cut(s) 319, 969
BseRI GAGGAG 1 cut(s) 115
BseSI GKGCMC 1 cut(s) 400
BseXI GCAGC 2 cut(s) 450, 1046
BshFI GGCC 2 cut(s) 5, 398
BshVI ATCGAT 1 cut(s) 433
BsiHKAI GWGCWC 1 cut(s) 832
BsiHKCI CYCGRG 1 cut(s) 456
BsiSI CCGG 1 cut(s) 126
BslI CCNNNNNNNGG 2 cut(s) 63, 473
BsmAI GTCTC 1 cut(s) 72
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 2 cut(s) 5, 398
Bso31I GGTCTC 1 cut(s) 72
BsoBI CYCGRG 1 cut(s) 456
Bsp120I GGGCCC 1 cut(s) 396
Bsp1286I GDGCHC 2 cut(s) 400, 832
Bsp143I GATC 1 cut(s) 813
BspACI CCGC 3 cut(s) 144, 196, 222
BspANI GGCC 2 cut(s) 5, 398
BspCNI CTCAG 2 cut(s) 229, 887
BspDI ATCGAT 1 cut(s) 433
BspHI TCATGA 1 cut(s) 832
BspLI GGNNCC 3 cut(s) 104, 398, 815
BspMAI CTGCAG 1 cut(s) 1036
BspPI GGATC 2 cut(s) 808, 821
BspQI GCTCTTC 1 cut(s) 981
BspTNI GGTCTC 1 cut(s) 72
BsrI ACTGG 2 cut(s) 319, 969
BssMI GATC 1 cut(s) 813
Bst2UI CCWGG 1 cut(s) 811
Bst4CI ACNGT 1 cut(s) 40
Bst6I CTCTTC 2 cut(s) 75, 981
BstAPI GCANNNNNTGC 1 cut(s) 1040
BstC8I GCNNGC 2 cut(s) 19, 528
BstDEI CTNAG 2 cut(s) 237, 874
BstENI CCTNNNNNAGG 1 cut(s) 471
BstF5I GGATG 1 cut(s) 501
BstH2I RGCGCY 1 cut(s) 328
BstHHI GCGC 2 cut(s) 17, 327
BstKTI GATC 1 cut(s) 816
BstMAI GTCTC 1 cut(s) 72
BstMBI GATC 1 cut(s) 813
BstMWI GCNNNNNNNGC 2 cut(s) 958, 1040
BstNI CCWGG 1 cut(s) 811
BstNSI RCATGY 1 cut(s) 1029
BstSCI CCNGG 2 cut(s) 124, 809
BstSFI CTRYAG 2 cut(s) 799, 1032
BstSLI GKGCMC 1 cut(s) 400
BstV1I GCAGC 2 cut(s) 450, 1046
BstX2I RGATCY 1 cut(s) 813
BstYI RGATCY 1 cut(s) 813
Bsu15I ATCGAT 1 cut(s) 433
BsuI GTATCC 1 cut(s) 774
BsuRI GGCC 2 cut(s) 5, 398
BsuTUI ATCGAT 1 cut(s) 433
BtgZI GCGATG 1 cut(s) 1006
BtsCI GGATG 1 cut(s) 501
BtsIMutI CAGTG 1 cut(s) 45
Cac8I GCNNGC 2 cut(s) 19, 528
CaiI CAGNNNCTG 1 cut(s) 1040
CciI TCATGA 1 cut(s) 832
CfoI GCGC 2 cut(s) 17, 327
Cfr13I GGNCC 3 cut(s) 396, 397, 745
ClaI ATCGAT 1 cut(s) 433
CseI GACGC 1 cut(s) 49
Csp6I GTAC 1 cut(s) 76
CspCI CAANNNNNGTGG 2 cut(s) 214, 249
CviAII CATG 3 cut(s) 329, 833, 1026
CviQI GTAC 1 cut(s) 76
DdeI CTNAG 2 cut(s) 237, 874
DpnI GATC 1 cut(s) 815
DpnII GATC 1 cut(s) 813
DraI TTTAAA 1 cut(s) 364
Eam1104I CTCTTC 2 cut(s) 75, 981
EarI CTCTTC 2 cut(s) 75, 981
EciI GGCGGA 1 cut(s) 211
Eco24I GRGCYC 1 cut(s) 400
Eco31I GGTCTC 1 cut(s) 72
Eco47I GGWCC 1 cut(s) 745
Eco57I CTGAAG 5 cut(s) 56, 184, 422, 465, 1002
Eco88I CYCGRG 1 cut(s) 456
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoO109I RGGNCCY 2 cut(s) 397, 745
EcoRII CCWGG 1 cut(s) 809
EcoT38I GRGCYC 1 cut(s) 400
FaeI CATG 3 cut(s) 332, 836, 1029
FalI AAGNNNNNCTT 4 cut(s) 200, 232, 573, 605
FatI CATG 3 cut(s) 328, 832, 1025
FauI CCCGC 1 cut(s) 137
Fnu4HI GCNGC 2 cut(s) 464, 1035
FokI GGATG 1 cut(s) 508
FriOI GRGCYC 1 cut(s) 400
Fsp4HI GCNGC 2 cut(s) 464, 1035
FspBI CTAG 5 cut(s) 557, 659, 669, 749, 953
GlaI GCGC 2 cut(s) 16, 326
GluI GCNGC 2 cut(s) 464, 1035
HaeII RGCGCY 1 cut(s) 328
HaeIII GGCC 2 cut(s) 5, 398
HapII CCGG 1 cut(s) 126
HgaI GACGC 1 cut(s) 49
HhaI GCGC 2 cut(s) 17, 327
Hin1II CATG 3 cut(s) 332, 836, 1029
Hin6I GCGC 2 cut(s) 15, 325
HinP1I GCGC 2 cut(s) 15, 325
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HinfI GANTC 6 cut(s) 137, 182, 239, 454, 672, 905
HpaII CCGG 1 cut(s) 126
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 1 cut(s) 562
Hpy188I TCNGA 6 cut(s) 244, 382, 598, 682, 730, 910
Hpy188III TCNNGA 8 cut(s) 202, 277, 458, 659, 669, 713, 833, 941
Hpy8I GTNNAC 1 cut(s) 562
HpyAV CCTTC 9 cut(s) 42, 63, 169, 205, 440, 603, 603, 607, 612
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4IV ACGT 2 cut(s) 487, 786
HpyCH4V TGCA 5 cut(s) 193, 332, 482, 899, 1034
HpyF10VI GCNNNNNNNGC 2 cut(s) 958, 1040
HpyF3I CTNAG 2 cut(s) 237, 874
HpySE526I ACGT 2 cut(s) 487, 786
Hsp92II CATG 3 cut(s) 332, 836, 1029
HspAI GCGC 2 cut(s) 15, 325
Kzo9I GATC 1 cut(s) 813
LguI GCTCTTC 1 cut(s) 981
LmnI GCTCC 4 cut(s) 102, 122, 322, 1042
Lsp1109I GCAGC 2 cut(s) 450, 1046
LweI GCATC 1 cut(s) 674
MaeI CTAG 5 cut(s) 557, 659, 669, 749, 953
MaeII ACGT 2 cut(s) 487, 786
MaeIII GTNAC 4 cut(s) 352, 719, 858, 971
MalI GATC 1 cut(s) 815
MboI GATC 1 cut(s) 813
MboII GAAGA 8 cut(s) 62, 178, 181, 184, 356, 398, 995, 998
MfeI CAATTG 1 cut(s) 626
MflI RGATCY 1 cut(s) 813
MhlI GDGCHC 2 cut(s) 400, 832
MluCI AATT 9 cut(s) 347, 359, 376, 626, 686, 879, 926, 1013, 1045
MlyI GAGTC 3 cut(s) 248, 448, 899
MmeI TCCRAC 2 cut(s) 222, 705
MnlI CCTC 6 cut(s) 93, 98, 477, 508, 688, 736
MseI TTAA 3 cut(s) 363, 707, 1069
MspI CCGG 1 cut(s) 126
MspR9I CCNGG 2 cut(s) 126, 811
MunI CAATTG 1 cut(s) 626
Mva1269I GAATGC 1 cut(s) 47
MvaI CCWGG 1 cut(s) 811
MwoI GCNNNNNNNGC 2 cut(s) 958, 1040
NciI CCSGG 1 cut(s) 126
NdeII GATC 1 cut(s) 813
NlaIII CATG 3 cut(s) 332, 836, 1029
NlaIV GGNNCC 3 cut(s) 104, 398, 815
NmuCI GTSAC 1 cut(s) 719
NspI RCATGY 1 cut(s) 1029
PaeR7I CTCGAG 1 cut(s) 456
PagI TCATGA 1 cut(s) 832
PciI ACATGT 1 cut(s) 1025
PciSI GCTCTTC 1 cut(s) 981
PctI GAATGC 1 cut(s) 47
PfeI GAWTC 3 cut(s) 137, 182, 672
PfoI TCCNGGA 1 cut(s) 809
PkrI GCNGC 2 cut(s) 465, 1036
PleI GAGTC 3 cut(s) 247, 448, 899
PpsI GAGTC 3 cut(s) 247, 448, 899
PpuMI RGGWCCY 1 cut(s) 745
PscI ACATGT 1 cut(s) 1025
Psp5II RGGWCCY 1 cut(s) 745
Psp6I CCWGG 1 cut(s) 809
PspGI CCWGG 1 cut(s) 809
PspN4I GGNNCC 3 cut(s) 104, 398, 815
PspOMI GGGCCC 1 cut(s) 396
PspPI GGNCC 3 cut(s) 396, 397, 745
PspPPI RGGWCCY 1 cut(s) 745
PstI CTGCAG 1 cut(s) 1036
PstNI CAGNNNCTG 1 cut(s) 1040
PsuI RGATCY 1 cut(s) 813
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
SapI GCTCTTC 1 cut(s) 981
SaqAI TTAA 3 cut(s) 363, 707, 1069
SatI GCNGC 2 cut(s) 464, 1035
Sau3AI GATC 1 cut(s) 813
Sau96I GGNCC 3 cut(s) 396, 397, 745
ScaI AGTACT 1 cut(s) 77
SchI GAGTC 3 cut(s) 248, 448, 899
ScrFI CCNGG 2 cut(s) 126, 811
SduI GDGCHC 2 cut(s) 400, 832
SfaNI GCATC 1 cut(s) 674
SfcI CTRYAG 2 cut(s) 799, 1032
Sfr274I CTCGAG 1 cut(s) 456
SinI GGWCC 1 cut(s) 745
SlaI CTCGAG 1 cut(s) 456
SmlI CTYRAG 2 cut(s) 456, 941
SmoI CTYRAG 2 cut(s) 456, 941
SpeI ACTAGT 1 cut(s) 556
Sse9I AATT 9 cut(s) 347, 359, 376, 626, 686, 879, 926, 1013, 1045
SsiI CCGC 3 cut(s) 144, 196, 222
SspMI CTAG 5 cut(s) 557, 659, 669, 749, 953
StyD4I CCNGG 2 cut(s) 124, 809
TaaI ACNGT 1 cut(s) 40
TaiI ACGT 2 cut(s) 490, 789
TaqI TCGA 2 cut(s) 433, 457
TasI AATT 9 cut(s) 347, 359, 376, 626, 686, 879, 926, 1013, 1045
TatI WGTACW 1 cut(s) 75
TfiI GAWTC 3 cut(s) 137, 182, 672
Tru1I TTAA 3 cut(s) 363, 707, 1069
Tru9I TTAA 3 cut(s) 363, 707, 1069
TscAI CASTG 1 cut(s) 45
TseFI GTSAC 1 cut(s) 719
TseI GCWGC 2 cut(s) 463, 1034
Tsp45I GTSAC 1 cut(s) 719
TspDTI ATGAA 2 cut(s) 323, 747
TspRI CASTG 1 cut(s) 45
VpaK11BI GGWCC 1 cut(s) 745
XagI CCTNNNNNAGG 1 cut(s) 471
XapI RAATTY 3 cut(s) 347, 359, 1013
XbaI TCTAGA 2 cut(s) 658, 668
XceI RCATGY 1 cut(s) 1029
XcmI CCANNNNNNNNNTGG 1 cut(s) 335
XhoI CTCGAG 1 cut(s) 456
XspI CTAG 5 cut(s) 557, 659, 669, 749, 953
ZrmI AGTACT 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.