Rh5DG422900

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002756
Physical Location & Seq
Forward (+)
67459018 .. 67479138
20121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG422900.1

Sequence Viewer

Length: 1101 bp
ATGACAGAGTCGCCTTCCGACTCACGACTCTTGGATCCTCATCATCCTTATCCTCATCTTGTGCCCCAAATCCCAATTGAGATTGTGAGCGACGAAGAGATGGCCTTGCTCTTCGCTCGCTCTCTAATCCCTTCCACAGTGAGAGCATTCCCTTCCAGCGTCAGGTCTCTTCAGTACTCCATCACCCTCCTTTCCAAGAGGAGCCTTTCGGGTTGCTCCCAACCCGGTATAGAGGATTCGGGCGGGTTCAACACTACTACCCAGAAGAAGAAGGCTGAATCGCTTTTGCACCGCTTCAGGAACAAGAAGGGCTTGTCCGCCTCCGATTTTGCTGAATCGGAATGGTGTGAAAGACAAATGCTGTACGTTCTCCTGAATGGCAACAGAGTAATCAGTAAGGCTATGAAAACTGGTAGGGAGCGCCATGCAAAACTTGAAGAAGAGTTTGTAACAAAATCTAAAGTTGTTGTCAATTCAGACGAAGATAAATGGGCCCTGAGGTTTTTGAAGTTTATTACAGGCATAAATCAATTATATACTGAAGGATTGACTCGAGAGCTGCCTCTCGTAGGTTTTGCAGAAGGTATATGGATGGTGGGAGTGATTGATGAGGTTCAAATGCCTGCCACAGAAACCCAGAGAAATCCATTATTAGTTGACACAAAGACTCGTGTGAAAGATAAACTTCCTTCCGAACCACAACGAAGGAAGGGAAGGTTTCAATTGATGTTCTACAAGTATTTGTGGGATAATCTAGAAGCATCTGGATTCCCCGCCGACCAATTCTTTGATGATTTTTCCTTAAATCCTGATTGTGACTTATCTGAAGAAATCAGGGAGGTCGTCCGAGCTAAATCAGGGTATCCAGCAAAGACCCTTGATGACGTGGTGAAATACTATAGAAAATCCTGGGTCCTGCTACCCCGTGCTCATGACCAACTTTTATTGAGCCCAGTAGATGCCCACCAGGTGTTCGATATAATGCCTCTTACAGCTGACTTATGTGGAAACAAATGCGCGTGTGATGCTGAACCATTCTTTGAACTGGTAAGGGGAAAAGTTGGACTTCTTTCAATCATCTTTGTGGAGTTGTACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

366

Amino Acids

41.69

Weight (kDa)

6.06

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 94 - 210 1e-30 Exonuclease V - a 5' deoxyribonuclease
Exo5 PF09810 211 - 284 1.1e-08 Exonuclease V - a 5' deoxyribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1019
AciI CCGC 4 cut(s) 243, 292, 318, 774
AclWI GGATC 2 cut(s) 29, 42
AcuI CTGAAG 4 cut(s) 155, 280, 561, 846
AdeI CACNNNGTG 1 cut(s) 970
AfaI GTAC 3 cut(s) 176, 365, 1094
AfiI CCNNNNNNNGG 2 cut(s) 162, 569
AgsI TTSAA 7 cut(s) 250, 437, 508, 617, 722, 1043, 1074
AjiI CACGTC 1 cut(s) 886
AjnI CCWGG 2 cut(s) 908, 966
AluBI AGCT 3 cut(s) 559, 851, 995
AluI AGCT 3 cut(s) 559, 851, 995
Alw21I GWGCWC 1 cut(s) 931
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 2 cut(s) 29, 42
Ama87I CYCGRG 1 cut(s) 552
AoxI GGCC 2 cut(s) 102, 492
ApaI GGGCCC 1 cut(s) 496
ApeKI GCWGC 1 cut(s) 559
ArsI GACNNNNNNTTYG 2 cut(s) 770, 802
AspLEI GCGC 2 cut(s) 423, 1019
AspS9I GGNCC 3 cut(s) 492, 493, 913
AsuC2I CCSGG 1 cut(s) 225
AsuHPI GGTGA 2 cut(s) 175, 901
AvaI CYCGRG 1 cut(s) 552
AvaII GGWCC 1 cut(s) 913
AxyI CCTNAGG 1 cut(s) 497
BaeGI GKGCMC 2 cut(s) 66, 496
BamHI GGATCC 1 cut(s) 34
BanII GRGCYC 2 cut(s) 496, 953
BauI CACGAG 1 cut(s) 669
Bbv12I GWGCWC 1 cut(s) 931
BbvI GCAGC 1 cut(s) 546
BccI CCATC 3 cut(s) 94, 188, 586
BciT130I CCWGG 2 cut(s) 910, 968
BciVI GTATCC 1 cut(s) 873
BcnI CCSGG 1 cut(s) 225
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 755
BfmI CTRYAG 1 cut(s) 898
BfoI RGCGCY 1 cut(s) 424
BfuI GTATCC 1 cut(s) 873
BisI GCNGC 1 cut(s) 560
BlsI GCNGC 1 cut(s) 561
BmcAI AGTACT 1 cut(s) 176
Bme1390I CCNGG 3 cut(s) 225, 910, 968
Bme18I GGWCC 1 cut(s) 913
BmeT110I CYCGRG 1 cut(s) 552
BmgBI CACGTC 1 cut(s) 886
BmgT120I GGNCC 3 cut(s) 492, 493, 913
BmiI GGNNCC 4 cut(s) 36, 203, 494, 914
BmrFI CCNGG 3 cut(s) 225, 910, 968
BmrI ACTGGG 1 cut(s) 947
BmsI GCATC 3 cut(s) 770, 949, 1015
BmuI ACTGGG 1 cut(s) 947
BpuMI CCSGG 1 cut(s) 225
BsaBI GATNNNNATC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 909
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 569
Bse1I ACTGG 3 cut(s) 415, 953, 1050
Bse21I CCTNAGG 1 cut(s) 497
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 2 cut(s) 910, 968
BseDI CCNNGG 1 cut(s) 909
BseGI GGATG 2 cut(s) 43, 597
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 2 cut(s) 162, 569
BseMII CTCAG 1 cut(s) 488
BseNI ACTGG 3 cut(s) 415, 953, 1050
BseRI GAGGAG 1 cut(s) 214
BseSI GKGCMC 2 cut(s) 66, 496
BseXI GCAGC 1 cut(s) 546
Bsh1236I CGCG 1 cut(s) 1019
BshFI GGCC 2 cut(s) 104, 494
BsiHKAI GWGCWC 1 cut(s) 931
BsiHKCI CYCGRG 1 cut(s) 552
BsiSI CCGG 1 cut(s) 225
BslI CCNNNNNNNGG 2 cut(s) 162, 569
BsmAI GTCTC 1 cut(s) 171
BsmI GAATGC 1 cut(s) 146
BsnI GGCC 2 cut(s) 104, 494
Bso31I GGTCTC 1 cut(s) 171
BsoBI CYCGRG 1 cut(s) 552
Bsp120I GGGCCC 1 cut(s) 492
Bsp1286I GDGCHC 4 cut(s) 66, 496, 931, 953
Bsp143I GATC 1 cut(s) 34
BspACI CCGC 4 cut(s) 243, 292, 318, 774
BspANI GGCC 2 cut(s) 104, 494
BspCNI CTCAG 1 cut(s) 489
BspFNI CGCG 1 cut(s) 1019
BspHI TCATGA 1 cut(s) 931
BspLI GGNNCC 4 cut(s) 36, 203, 494, 914
BspPI GGATC 2 cut(s) 29, 42
BspQI GCTCTTC 1 cut(s) 116
BspTNI GGTCTC 1 cut(s) 171
BsrI ACTGG 3 cut(s) 415, 953, 1050
BssECI CCNNGG 1 cut(s) 909
BssMI GATC 1 cut(s) 34
BssSI CACGAG 1 cut(s) 669
Bst2BI CACGAG 1 cut(s) 669
Bst2UI CCWGG 2 cut(s) 910, 968
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 4 cut(s) 90, 116, 174, 435
BstC8I GCNNGC 2 cut(s) 118, 624
BstDEI CTNAG 1 cut(s) 497
BstENI CCTNNNNNAGG 1 cut(s) 567
BstF5I GGATG 2 cut(s) 43, 597
BstFNI CGCG 1 cut(s) 1019
BstH2I RGCGCY 1 cut(s) 424
BstHHI GCGC 2 cut(s) 423, 1019
BstKTI GATC 1 cut(s) 37
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 1 cut(s) 1025
BstNI CCWGG 2 cut(s) 910, 968
BstSCI CCNGG 3 cut(s) 223, 908, 966
BstSFI CTRYAG 1 cut(s) 898
BstSLI GKGCMC 2 cut(s) 66, 496
BstUI CGCG 1 cut(s) 1019
BstV1I GCAGC 1 cut(s) 546
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
Bsu36I CCTNAGG 1 cut(s) 497
BsuI GTATCC 1 cut(s) 873
BsuRI GGCC 2 cut(s) 104, 494
BtrI CACGTC 1 cut(s) 886
BtsCI GGATG 2 cut(s) 43, 597
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 2 cut(s) 118, 624
CciI TCATGA 1 cut(s) 931
CfoI GCGC 2 cut(s) 423, 1019
Cfr13I GGNCC 3 cut(s) 492, 493, 913
CseI GACGC 1 cut(s) 148
CsiI ACCWGGT 1 cut(s) 966
Csp6I GTAC 3 cut(s) 175, 364, 1093
CviAII CATG 2 cut(s) 425, 932
CviQI GTAC 3 cut(s) 175, 364, 1093
DdeI CTNAG 1 cut(s) 497
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
DraIII CACNNNGTG 1 cut(s) 970
Eam1104I CTCTTC 4 cut(s) 90, 116, 174, 435
EarI CTCTTC 4 cut(s) 90, 116, 174, 435
EciI GGCGGA 1 cut(s) 307
Eco24I GRGCYC 2 cut(s) 496, 953
Eco31I GGTCTC 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 913
Eco57I CTGAAG 4 cut(s) 155, 280, 561, 846
Eco81I CCTNAGG 1 cut(s) 497
Eco88I CYCGRG 1 cut(s) 552
EcoNI CCTNNNNNAGG 1 cut(s) 567
EcoO109I RGGNCCY 2 cut(s) 493, 913
EcoRII CCWGG 2 cut(s) 908, 966
EcoT38I GRGCYC 2 cut(s) 496, 953
FaeI CATG 2 cut(s) 428, 935
FalI AAGNNNNNCTT 6 cut(s) 296, 328, 669, 701, 1050, 1082
FatI CATG 2 cut(s) 424, 931
FauI CCCGC 2 cut(s) 236, 781
Fnu4HI GCNGC 1 cut(s) 560
FokI GGATG 2 cut(s) 30, 604
FriOI GRGCYC 2 cut(s) 496, 953
Fsp4HI GCNGC 1 cut(s) 560
FspBI CTAG 1 cut(s) 755
GlaI GCGC 2 cut(s) 422, 1018
GluI GCNGC 1 cut(s) 560
HaeII RGCGCY 1 cut(s) 424
HaeIII GGCC 2 cut(s) 104, 494
HapII CCGG 1 cut(s) 225
HgaI GACGC 1 cut(s) 148
HhaI GCGC 2 cut(s) 423, 1019
Hin1II CATG 2 cut(s) 428, 935
Hin6I GCGC 2 cut(s) 421, 1017
HinP1I GCGC 2 cut(s) 421, 1017
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HinfI GANTC 9 cut(s) 8, 20, 27, 236, 278, 335, 550, 667, 768
HpaII CCGG 1 cut(s) 225
HphI GGTGA 2 cut(s) 175, 901
Hpy166II GTNNAC 1 cut(s) 658
Hpy188I TCNGA 7 cut(s) 19, 325, 340, 478, 694, 826, 848
Hpy188III TCNNGA 8 cut(s) 24, 298, 373, 554, 755, 765, 809, 932
Hpy8I GTNNAC 1 cut(s) 658
Hpy99I CGWCG 1 cut(s) 95
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4IV ACGT 2 cut(s) 366, 885
HpyCH4V TGCA 3 cut(s) 289, 428, 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 1025
HpyF3I CTNAG 1 cut(s) 497
HpySE526I ACGT 2 cut(s) 366, 885
Hsp92II CATG 2 cut(s) 428, 935
HspAI GCGC 2 cut(s) 421, 1017
Kzo9I GATC 1 cut(s) 34
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 3 cut(s) 201, 221, 418
Lsp1109I GCAGC 1 cut(s) 546
LweI GCATC 3 cut(s) 770, 949, 1015
MabI ACCWGGT 1 cut(s) 966
MaeI CTAG 1 cut(s) 755
MaeII ACGT 2 cut(s) 366, 885
MaeIII GTNAC 2 cut(s) 448, 815
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MboII GAAGA 9 cut(s) 103, 107, 161, 277, 280, 449, 452, 494, 839
MfeI CAATTG 2 cut(s) 75, 722
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 4 cut(s) 66, 496, 931, 953
MluCI AATT 5 cut(s) 75, 472, 530, 722, 782
MlyI GAGTC 5 cut(s) 14, 17, 21, 544, 661
MmeI TCCRAC 2 cut(s) 42, 1042
MseI TTAA 1 cut(s) 803
MslI CAYNNNNRTG 1 cut(s) 1082
MspA1I CMGCKG 1 cut(s) 995
MspI CCGG 1 cut(s) 225
MspR9I CCNGG 3 cut(s) 225, 910, 968
MunI CAATTG 2 cut(s) 75, 722
Mva1269I GAATGC 1 cut(s) 146
MvaI CCWGG 2 cut(s) 910, 968
MvnI CGCG 1 cut(s) 1019
MwoI GCNNNNNNNGC 1 cut(s) 1025
NciI CCSGG 1 cut(s) 225
NdeII GATC 1 cut(s) 34
NlaIII CATG 2 cut(s) 428, 935
NlaIV GGNNCC 4 cut(s) 36, 203, 494, 914
NmuCI GTSAC 1 cut(s) 815
PaeR7I CTCGAG 1 cut(s) 552
PagI TCATGA 1 cut(s) 931
PciSI GCTCTTC 1 cut(s) 116
PctI GAATGC 1 cut(s) 146
PfeI GAWTC 4 cut(s) 236, 278, 335, 768
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 1 cut(s) 561
PleI GAGTC 5 cut(s) 14, 16, 21, 544, 661
PpsI GAGTC 5 cut(s) 14, 16, 21, 544, 661
PpuMI RGGWCCY 1 cut(s) 913
Psp5II RGGWCCY 1 cut(s) 913
Psp6I CCWGG 2 cut(s) 908, 966
PspGI CCWGG 2 cut(s) 908, 966
PspN4I GGNNCC 4 cut(s) 36, 203, 494, 914
PspOMI GGGCCC 1 cut(s) 492
PspPI GGNCC 3 cut(s) 492, 493, 913
PspPPI RGGWCCY 1 cut(s) 913
PsuI RGATCY 1 cut(s) 34
PsyI GACNNNGTC 1 cut(s) 7
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 3 cut(s) 176, 365, 1094
RsaNI GTAC 3 cut(s) 175, 364, 1093
RseI CAYNNNNRTG 1 cut(s) 1082
SapI GCTCTTC 1 cut(s) 116
SaqAI TTAA 1 cut(s) 803
SatI GCNGC 1 cut(s) 560
Sau3AI GATC 1 cut(s) 34
Sau96I GGNCC 3 cut(s) 492, 493, 913
ScaI AGTACT 1 cut(s) 176
SchI GAGTC 5 cut(s) 14, 17, 21, 544, 661
ScrFI CCNGG 3 cut(s) 225, 910, 968
SduI GDGCHC 4 cut(s) 66, 496, 931, 953
SexAI ACCWGGT 1 cut(s) 966
SfaNI GCATC 3 cut(s) 770, 949, 1015
SfcI CTRYAG 1 cut(s) 898
Sfr274I CTCGAG 1 cut(s) 552
SinI GGWCC 1 cut(s) 913
SlaI CTCGAG 1 cut(s) 552
SmiMI CAYNNNNRTG 1 cut(s) 1082
SmlI CTYRAG 1 cut(s) 552
SmoI CTYRAG 1 cut(s) 552
Sse9I AATT 5 cut(s) 75, 472, 530, 722, 782
SsiI CCGC 4 cut(s) 243, 292, 318, 774
SspMI CTAG 1 cut(s) 755
StyD4I CCNGG 3 cut(s) 223, 908, 966
TaaI ACNGT 1 cut(s) 139
TaiI ACGT 2 cut(s) 369, 888
TaqI TCGA 2 cut(s) 553, 975
TasI AATT 5 cut(s) 75, 472, 530, 722, 782
TatI WGTACW 1 cut(s) 174
TfiI GAWTC 4 cut(s) 236, 278, 335, 768
Tru1I TTAA 1 cut(s) 803
Tru9I TTAA 1 cut(s) 803
TscAI CASTG 1 cut(s) 144
TseFI GTSAC 1 cut(s) 815
TseI GCWGC 1 cut(s) 559
Tsp45I GTSAC 1 cut(s) 815
TspDTI ATGAA 1 cut(s) 419
TspRI CASTG 1 cut(s) 144
Tth111I GACNNNGTC 1 cut(s) 7
VpaK11BI GGWCC 1 cut(s) 913
XagI CCTNNNNNAGG 1 cut(s) 567
XbaI TCTAGA 1 cut(s) 754
XhoI CTCGAG 1 cut(s) 552
XspI CTAG 1 cut(s) 755
ZrmI AGTACT 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.