MD03G1105200.v1.1

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002756
Physical Location & Seq
Forward (+)
9004148 .. 9007683
3536 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1105200.v1.1.491

Sequence Viewer

Length: 1227 bp
ATGACCGAGTCACCGTCCGAGTCAATCTCCGACTCACCTTTCAACCACAGCAATAAGGGGAACGATGCCATCGTGCCAGAAATCCCAATCGAGATTGTGAGCGAGGAAGAGATGGCTCTGCTCGAAGCTGCTATTGTTTCCGCTCGCGCTTCCTTCTCTGCCATCCCTGCAATTCGGTCCTCAATGCTTTCGTTCCACGGCAACGTTAGGTCTATTCAGTCGATCACCGCTTTGTCGAAGAGGAGGCTTTCGGGTTGTTCCGAACCCGATATTGAGGATTCGGGTGGGTTGACGAGTGCCCAGAAGAAGACTCGGGTGGCTGACTCGTTTTTGCACCGGTTTAGGAAGAAGGGGTTGTATGTCACAGACATTACTGCCACGATGGGGTTTTTGGAATGGTGTGAAAAACAAATGGAGTTTGTTCTCCTGGTTGGCAAGCGAAAAGAGAGTAAGGCTATGAAAGCGGGTATTGCTCGCCATGCAAAACTTGAAGAAGAGGTTGTAAAAAGAGTTAAAGTTCGTGTCACGTCGACTGAAGATAGATGGGCTCTGAAGCTTCTGAATTTTATAACTGGTGTAAATCAATTACTGTTTGAAGGATTGACACGTGAGCTGCCACTAATAGGTTTTGCAGAAGGGGTATGGATGGTGGGTGTGATCGATGAGATTCGAATGCCTGTTACGGAAACCAACCGACACCCAATATTAGTTGACACAAAGACTCGTGTGAAAGATACACTTCCTGCTGAACCACAACGAAGAAATGGAAGGCTTCAATTAATGTGCTACAAGTACATTTGGGATGATTTAGTTGCCGATAAATTCCCATCAGAGAAATTTTTCACTTCTTTTTCCTTAAATCCTCATGGCATCTTATCTGAAGAAATCAGAAAGATGACTGCTAACTCAGGGTTCCCAGCAGAGACCCTTGATGACATAGTGAGATACTATAGAAACACATGTAGCATGCTGCCCTCTGCACATGACCAGCTGGTATTGAGATATGAGTTACAGAAAGATCATTCGTTACTTGGTGAAGATGAGTTTGCATACGACTCTGATTGGGTCAAGAATCAAATACAGGGTTGTCTTGAGTTCTGGTTAGGAGAGCGAGAAGCAAGTTACACTCCTGAGGATGAGCGATGGAAATGCCGGTTCTGTCAATATTCTTCTATCTGCCCCGCTGAAAGTGCGGAGTCTGCGGACTATAATAATACACCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

409

Amino Acids

46.29

Weight (kDa)

5.57

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 110 - 225 1.4e-30 Exonuclease V - a 5' deoxyribonuclease
PDDEXK_1 PF12705 177 - 394 4.9e-08 PD-(D/E)XK nuclease superfamily
Exo5 PF09810 231 - 298 6.5e-08 Exonuclease V - a 5' deoxyribonuclease
Exo5 PF09810 323 - 393 7e-11 Exonuclease V - a 5' deoxyribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 569
AccBSI CCGCTC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 530
AccII CGCG 1 cut(s) 147
AciI CCGC 6 cut(s) 141, 228, 464, 1182, 1193, 1202
AclI AACGTT 1 cut(s) 204
AcsI RAATTY 3 cut(s) 562, 821, 836
AcuI CTGAAG 3 cut(s) 555, 572, 900
AcvI CACGTG 1 cut(s) 608
AfaI GTAC 1 cut(s) 794
AfiI CCNNNNNNNGG 2 cut(s) 384, 623
AflIII ACRYGT 2 cut(s) 605, 959
AgeI ACCGGT 1 cut(s) 336
AgsI TTSAA 4 cut(s) 43, 491, 596, 776
AjiI CACGTC 1 cut(s) 528
AjnI CCWGG 1 cut(s) 426
AluBI AGCT 4 cut(s) 128, 556, 613, 991
AluI AGCT 4 cut(s) 128, 556, 613, 991
Alw26I GTCTC 1 cut(s) 917
Ama87I CYCGRG 1 cut(s) 312
ApeKI GCWGC 3 cut(s) 128, 613, 970
ApoI RAATTY 3 cut(s) 562, 821, 836
AseI ATTAAT 1 cut(s) 779
AsiGI ACCGGT 1 cut(s) 336
Asp700I GAANNNNTTC 1 cut(s) 839
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 177
AsuHPI GGTGA 4 cut(s) 3, 27, 217, 1046
AsuII TTCGAA 1 cut(s) 670
AvaI CYCGRG 1 cut(s) 312
AvaII GGWCC 1 cut(s) 177
AxyI CCTNAGG 1 cut(s) 1131
BaeGI GKGCMC 1 cut(s) 301
BanII GRGCYC 1 cut(s) 550
BauI CACGAG 1 cut(s) 723
BbrPI CACGTG 1 cut(s) 608
BbsI GAAGAC 1 cut(s) 314
BbvI GCAGC 3 cut(s) 115, 600, 957
BccI CCATC 8 cut(s) 77, 106, 170, 376, 537, 640, 835, 1137
BceAI ACGGC 1 cut(s) 214
BcgI CGANNNNNNTGC 2 cut(s) 1131, 1165
BciT130I CCWGG 1 cut(s) 428
BcoDI GTCTC 1 cut(s) 917
BfmI CTRYAG 1 cut(s) 949
BisI GCNGC 3 cut(s) 129, 614, 971
BlsI GCNGC 3 cut(s) 130, 615, 972
Bme1390I CCNGG 1 cut(s) 428
Bme18I GGWCC 1 cut(s) 177
BmeT110I CYCGRG 1 cut(s) 312
BmgBI CACGTC 1 cut(s) 528
BmgT120I GGNCC 1 cut(s) 177
BmiI GGNNCC 1 cut(s) 914
BmrFI CCNGG 1 cut(s) 428
BmsI GCATC 2 cut(s) 55, 879
BpiI GAAGAC 1 cut(s) 314
BplI GAGNNNNNCTC 2 cut(s) 11, 43
Bpu14I TTCGAA 1 cut(s) 670
BpuEI CTTGAG 1 cut(s) 1112
Bsa29I ATCGAT 1 cut(s) 660
BsaAI YACGTR 1 cut(s) 608
BsaI GGTCTC 1 cut(s) 917
BsaJI CCNNGG 1 cut(s) 196
BsaWI WCCGGW 1 cut(s) 336
Bsc4I CCNNNNNNNGG 2 cut(s) 384, 623
Bse118I RCCGGY 2 cut(s) 336, 1152
Bse1I ACTGG 1 cut(s) 577
Bse21I CCTNAGG 1 cut(s) 1131
BseBI CCWGG 1 cut(s) 428
BseCI ATCGAT 1 cut(s) 660
BseDI CCNNGG 1 cut(s) 196
BseGI GGATG 4 cut(s) 162, 651, 808, 1141
BseLI CCNNNNNNNGG 2 cut(s) 384, 623
BseMII CTCAG 2 cut(s) 921, 1122
BseNI ACTGG 1 cut(s) 577
BseRI GAGGAG 1 cut(s) 256
BseSI GKGCMC 1 cut(s) 301
BseXI GCAGC 3 cut(s) 115, 600, 957
BseYI CCCAGC 1 cut(s) 916
BsgI GTGCAG 1 cut(s) 963
Bsh1236I CGCG 1 cut(s) 147
BshTI ACCGGT 1 cut(s) 336
BshVI ATCGAT 1 cut(s) 660
BsiHKCI CYCGRG 1 cut(s) 312
BsiSI CCGG 2 cut(s) 337, 1153
BslI CCNNNNNNNGG 2 cut(s) 384, 623
BsmAI GTCTC 1 cut(s) 917
BsmI GAATGC 1 cut(s) 678
Bso31I GGTCTC 1 cut(s) 917
BsoBI CYCGRG 1 cut(s) 312
Bsp119I TTCGAA 1 cut(s) 670
Bsp1286I GDGCHC 2 cut(s) 301, 550
Bsp143I GATC 3 cut(s) 222, 657, 1018
BspACI CCGC 6 cut(s) 141, 228, 464, 1182, 1193, 1202
BspCNI CTCAG 2 cut(s) 920, 1123
BspDI ATCGAT 1 cut(s) 660
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 1 cut(s) 914
BspT104I TTCGAA 1 cut(s) 670
BspTNI GGTCTC 1 cut(s) 917
BsrBI CCGCTC 1 cut(s) 143
BsrFI RCCGGY 2 cut(s) 336, 1152
BsrI ACTGG 1 cut(s) 577
BssAI RCCGGY 2 cut(s) 336, 1152
BssECI CCNNGG 1 cut(s) 196
BssMI GATC 3 cut(s) 222, 657, 1018
BssSI CACGAG 1 cut(s) 723
Bst2BI CACGAG 1 cut(s) 723
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 2 cut(s) 15, 591
Bst6I CTCTTC 3 cut(s) 102, 233, 489
BstBAI YACGTR 1 cut(s) 608
BstBI TTCGAA 1 cut(s) 670
BstC8I GCNNGC 4 cut(s) 145, 437, 475, 968
BstDEI CTNAG 2 cut(s) 907, 1131
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 4 cut(s) 162, 651, 808, 1141
BstFNI CGCG 1 cut(s) 147
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 3 cut(s) 225, 660, 1021
BstMAI GTCTC 1 cut(s) 917
BstMBI GATC 3 cut(s) 222, 657, 1018
BstMWI GCNNNNNNNGC 6 cut(s) 167, 461, 470, 479, 1190, 1199
BstNI CCWGG 1 cut(s) 428
BstNSI RCATGY 2 cut(s) 963, 970
BstSCI CCNGG 1 cut(s) 426
BstSFI CTRYAG 1 cut(s) 949
BstSLI GKGCMC 1 cut(s) 301
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 3 cut(s) 115, 600, 957
BstV2I GAAGAC 1 cut(s) 314
Bsu15I ATCGAT 1 cut(s) 660
Bsu36I CCTNAGG 1 cut(s) 1131
BsuTUI ATCGAT 1 cut(s) 660
BtgI CCRYGG 1 cut(s) 196
BtgZI GCGATG 1 cut(s) 1156
BtrI CACGTC 1 cut(s) 528
BtsCI GGATG 4 cut(s) 162, 651, 808, 1141
Cac8I GCNNGC 4 cut(s) 145, 437, 475, 968
CfoI GCGC 1 cut(s) 149
Cfr10I RCCGGY 2 cut(s) 336, 1152
Cfr13I GGNCC 1 cut(s) 177
ClaI ATCGAT 1 cut(s) 660
Csp6I GTAC 1 cut(s) 793
CspAI ACCGGT 1 cut(s) 336
CviAII CATG 5 cut(s) 479, 866, 960, 967, 983
CviQI GTAC 1 cut(s) 793
DdeI CTNAG 2 cut(s) 907, 1131
DpnI GATC 3 cut(s) 224, 659, 1020
DpnII GATC 3 cut(s) 222, 657, 1018
Eam1104I CTCTTC 3 cut(s) 102, 233, 489
EarI CTCTTC 3 cut(s) 102, 233, 489
Eco24I GRGCYC 1 cut(s) 550
Eco31I GGTCTC 1 cut(s) 917
Eco47I GGWCC 1 cut(s) 177
Eco57I CTGAAG 3 cut(s) 555, 572, 900
Eco72I CACGTG 1 cut(s) 608
Eco81I CCTNAGG 1 cut(s) 1131
Eco88I CYCGRG 1 cut(s) 312
EcoRII CCWGG 1 cut(s) 426
EcoT38I GRGCYC 1 cut(s) 550
FaeI CATG 5 cut(s) 482, 869, 963, 970, 986
FalI AAGNNNNNCTT 2 cut(s) 723, 755
FatI CATG 5 cut(s) 478, 865, 959, 966, 982
FauI CCCGC 2 cut(s) 457, 1189
FblI GTMKAC 1 cut(s) 530
Fnu4HI GCNGC 3 cut(s) 129, 614, 971
FokI GGATG 4 cut(s) 149, 658, 815, 1148
FriOI GRGCYC 1 cut(s) 550
Fsp4HI GCNGC 3 cut(s) 129, 614, 971
GlaI GCGC 1 cut(s) 148
GluI GCNGC 3 cut(s) 129, 614, 971
GsaI CCCAGC 1 cut(s) 920
HapII CCGG 2 cut(s) 337, 1153
HhaI GCGC 1 cut(s) 149
Hin1II CATG 5 cut(s) 482, 869, 963, 970, 986
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 3 cut(s) 291, 531, 712
HindII GTYRAC 3 cut(s) 291, 531, 712
HindIII AAGCTT 1 cut(s) 554
HpaII CCGG 2 cut(s) 337, 1153
HphI GGTGA 4 cut(s) 3, 27, 217, 1046
Hpy166II GTNNAC 3 cut(s) 291, 531, 712
Hpy188I TCNGA 9 cut(s) 19, 31, 262, 552, 561, 832, 880, 890, 1060
Hpy188III TCNNGA 4 cut(s) 91, 1069, 1091, 1130
Hpy8I GTNNAC 3 cut(s) 291, 531, 712
Hpy99I CGWCG 1 cut(s) 532
HpyAV CCTTC 5 cut(s) 163, 343, 590, 629, 762
HpyCH4III ACNGT 2 cut(s) 15, 591
HpyCH4IV ACGT 3 cut(s) 204, 527, 607
HpyCH4V TGCA 6 cut(s) 170, 334, 482, 632, 980, 1049
HpyF10VI GCNNNNNNNGC 6 cut(s) 167, 461, 470, 479, 1190, 1199
HpyF3I CTNAG 2 cut(s) 907, 1131
HpySE526I ACGT 3 cut(s) 204, 527, 607
Hsp92II CATG 5 cut(s) 482, 869, 963, 970, 986
HspAI GCGC 1 cut(s) 147
Kzo9I GATC 3 cut(s) 222, 657, 1018
Lsp1109I GCAGC 3 cut(s) 115, 600, 957
LweI GCATC 2 cut(s) 55, 879
MaeII ACGT 3 cut(s) 204, 527, 607
MaeIII GTNAC 7 cut(s) 9, 361, 523, 679, 1008, 1026, 1121
MalI GATC 3 cut(s) 224, 659, 1020
MbiI CCGCTC 1 cut(s) 143
MboI GATC 3 cut(s) 222, 657, 1018
MhlI GDGCHC 2 cut(s) 301, 550
MluCI AATT 6 cut(s) 171, 562, 584, 776, 821, 836
MlyI GAGTC 8 cut(s) 17, 26, 29, 304, 317, 715, 1049, 1205
MmeI TCCRAC 1 cut(s) 54
MnlI CCTC 9 cut(s) 97, 190, 234, 237, 268, 490, 873, 985, 1126
MroXI GAANNNNTTC 1 cut(s) 839
MseI TTAA 3 cut(s) 513, 779, 857
MspA1I CMGCKG 2 cut(s) 991, 1184
MspI CCGG 2 cut(s) 337, 1153
MspR9I CCNGG 1 cut(s) 428
Mva1269I GAATGC 1 cut(s) 678
MvaI CCWGG 1 cut(s) 428
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 6 cut(s) 167, 461, 470, 479, 1190, 1199
NdeII GATC 3 cut(s) 222, 657, 1018
NlaIII CATG 5 cut(s) 482, 869, 963, 970, 986
NlaIV GGNNCC 1 cut(s) 914
NmuCI GTSAC 3 cut(s) 9, 361, 523
NspI RCATGY 2 cut(s) 963, 970
NspV TTCGAA 1 cut(s) 670
PaeI GCATGC 1 cut(s) 970
PciI ACATGT 1 cut(s) 959
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PctI GAATGC 1 cut(s) 678
PdmI GAANNNNTTC 1 cut(s) 839
PfeI GAWTC 3 cut(s) 278, 667, 1072
PflFI GACNNNGTC 1 cut(s) 7
PinAI ACCGGT 1 cut(s) 336
PkrI GCNGC 3 cut(s) 130, 615, 972
PleI GAGTC 8 cut(s) 16, 26, 28, 304, 317, 715, 1049, 1204
PmaCI CACGTG 1 cut(s) 608
PmlI CACGTG 1 cut(s) 608
PpsI GAGTC 8 cut(s) 16, 26, 28, 304, 317, 715, 1049, 1204
Ppu21I YACGTR 1 cut(s) 608
PscI ACATGT 1 cut(s) 959
PshBI ATTAAT 1 cut(s) 779
PsiI TTATAA 1 cut(s) 569
Psp1406I AACGTT 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 426
PspCI CACGTG 1 cut(s) 608
PspFI CCCAGC 1 cut(s) 916
PspGI CCWGG 1 cut(s) 426
PspN4I GGNNCC 1 cut(s) 914
PspPI GGNCC 1 cut(s) 177
PsyI GACNNNGTC 1 cut(s) 7
PvuII CAGCTG 1 cut(s) 991
RsaI GTAC 1 cut(s) 794
RsaNI GTAC 1 cut(s) 793
SalI GTCGAC 1 cut(s) 529
SaqAI TTAA 3 cut(s) 513, 779, 857
SatI GCNGC 3 cut(s) 129, 614, 971
Sau3AI GATC 3 cut(s) 222, 657, 1018
Sau96I GGNCC 1 cut(s) 177
SchI GAGTC 8 cut(s) 17, 26, 29, 304, 317, 715, 1049, 1205
ScrFI CCNGG 1 cut(s) 428
SduI GDGCHC 2 cut(s) 301, 550
SfaNI GCATC 2 cut(s) 55, 879
SfcI CTRYAG 1 cut(s) 949
SfuI TTCGAA 1 cut(s) 670
SinI GGWCC 1 cut(s) 177
SmlI CTYRAG 1 cut(s) 1091
SmoI CTYRAG 1 cut(s) 1091
SphI GCATGC 1 cut(s) 970
Sse9I AATT 6 cut(s) 171, 562, 584, 776, 821, 836
SsiI CCGC 6 cut(s) 141, 228, 464, 1182, 1193, 1202
SspI AATATT 2 cut(s) 705, 1166
StyD4I CCNGG 1 cut(s) 426
TaaI ACNGT 2 cut(s) 15, 591
TaiI ACGT 3 cut(s) 207, 530, 610
TaqI TCGA 7 cut(s) 90, 123, 221, 236, 530, 660, 670
TaqII GACCGA 2 cut(s) 20, 165
TasI AATT 6 cut(s) 171, 562, 584, 776, 821, 836
TatI WGTACW 1 cut(s) 792
TfiI GAWTC 3 cut(s) 278, 667, 1072
Tru1I TTAA 3 cut(s) 513, 779, 857
Tru9I TTAA 3 cut(s) 513, 779, 857
TseFI GTSAC 3 cut(s) 9, 361, 523
TseI GCWGC 3 cut(s) 128, 613, 970
Tsp45I GTSAC 3 cut(s) 9, 361, 523
TspDTI ATGAA 1 cut(s) 473
TspGWI ACGGA 1 cut(s) 698
Tth111I GACNNNGTC 1 cut(s) 7
VpaK11BI GGWCC 1 cut(s) 177
VspI ATTAAT 1 cut(s) 779
XapI RAATTY 3 cut(s) 562, 821, 836
XceI RCATGY 2 cut(s) 963, 970
XmiI GTMKAC 1 cut(s) 530
XmnI GAANNNNTTC 1 cut(s) 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.