Rorug05G0331100

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002756
Physical Location & Seq
Reverse (-)
40099007 .. 40100392
1386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0331100.1

Sequence Viewer

Length: 1386 bp
ATGGGTGACTCTCCTTCTTTCTCGTGGAGAAGCATTCTGAATAGTAGAGAGGTGCTAAAAGCCGGAATCCAATGGAGAGTAGGTGATGGTTCTCAGATCAGCATATGGCATGACAATTGGATTCCTCACTGCCCACAGTATCTTGTCCAGAAACCGCCCAATTGTCCTCTAACTCATGTCTCAGAACTTATTGATCCATCCACAAGCTCGTGGAATGTCCCCATACTTGCAGCACTTTTTACTCATGAGGTGGCTGCCAAGATCTTGTGCATTCCCCTAAGTCTTAGGGTATTGCATGATACAATCAGCTGGAAACCGGAGAAGCATGGATTTTACTCTGTCCGGTCAGCGTACTGGATAGCACGAGCTTCGGTCCTTGATCATGTCCTAGCCTCCTCTTCGAGCGGTGATCCCTACAGTGAGTTGTGGAAGAAGTTGTGGAAGGCAAGGGTTCCGGGTAAAGTGCATATATGTTTATGGCGAGCATGTCATAATTTGTTACCTACAAGAGCCAAGCTTGTATCGAAAGGATATACCGGGGAGGTGAACTGCATTTTATGCACTCACAGATTGGAAGATAATGCACATATTTTCTGTCAATGTCCTACTGCAGTGTCTATTCTTTCATCTCCTCCTTTTCATCTAAAGTCCACTTTGCTTCCACATATCAATTTTAAGGAATGGATGTTGGAACGTGCCATTCACCTCAATTCTGATCTCTTTGATAAGTTGATGATGGTGATTTGGTCCATGTGGAAAAACAGAATTAACAAGTTATGGAACAATTCATGTCGAACAGCTAATGACCTGGTTCTAAGCTCTTTATCATGGTTGGAGGAGTATCGAAAGGCTAGGCACAATGTTTGTAAGCCACGAACTTGCGGGAGTCAACGATGGAAGCCTGCAGAGGAGGGCCACTGGAAGTTAAATGTGGACGGCAGTTTCCTACCAAATCGGACTGCAGGGGGTATTGGCGCAGTCCTTCGGGATTCTCATGGCAGCTTCCGAGCAGCGGTGGCTACACCTGTGATACACATGGCGTCAGCCAAGCAGGTAGAACTGGTAGCCATTCAGCAAGGTCTTCAACTGCTGCAACAGATGCGAACCGAGCAGGTCGTGATCGAAACTGATTGTCTACAGGCCACTTTGGATATCCAGAATGCAAACCATGAGAGATTGCTTGAGGGAGGCTTAATTGATGATATCAAAATACTCATGCAGACTATGCCAATGGTCACTATTCGTTATGCACCAAGAACTTGTAATGCTGTGGCACATCGCCTAGCCAATATTGCTTATGAAGCAAGTAGTCCTTCCTTGTGGATTGATGACGCACCAGAATGTATTTTGGATGTGCTTAACTTCGATTGTAACCCTCCCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

461

Amino Acids

52.14

Weight (kDa)

8.54

Isoelectric Point (pI)

50.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 133 - 205 2.8e-15 zinc-binding in reverse transcriptase
RVT_3 PF13456 310 - 431 3.7e-22 Reverse transcriptase-like
RNase_H PF00075 311 - 386 3.8e-06 RNase H
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1042, 1102
AccBSI CCGCTC 1 cut(s) 405
AccI GTMKAC 1 cut(s) 1135
AciI CCGC 4 cut(s) 155, 405, 882, 1013
AclWI GGATC 2 cut(s) 188, 404
AcyI GRCGYC 1 cut(s) 1040
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 1012
AgsI TTSAA 1 cut(s) 1085
AjnI CCWGG 1 cut(s) 807
AluBI AGCT 7 cut(s) 207, 309, 368, 517, 800, 819, 1002
AluI AGCT 7 cut(s) 207, 309, 368, 517, 800, 819, 1002
Alw26I GTCTC 1 cut(s) 184
AlwI GGATC 2 cut(s) 188, 404
AoxI GGCC 2 cut(s) 913, 1140
ApeKI GCWGC 5 cut(s) 230, 254, 999, 1010, 1090
AspLEI GCGC 1 cut(s) 977
AspS9I GGNCC 3 cut(s) 373, 747, 913
AsuC2I CCSGG 2 cut(s) 456, 538
AsuHPI GGTGA 6 cut(s) 17, 95, 419, 556, 695, 751
AvaII GGWCC 2 cut(s) 373, 747
BauI CACGAG 3 cut(s) 22, 208, 363
BbsI GAAGAC 1 cut(s) 1073
BbvI GCAGC 5 cut(s) 241, 242, 1011, 1022, 1077
BccI CCATC 4 cut(s) 80, 205, 730, 888
BceAI ACGGC 1 cut(s) 952
BcgI CGANNNNNNTGC 2 cut(s) 351, 385
BciT130I CCWGG 1 cut(s) 809
BclI TGATCA 1 cut(s) 379
BcnI CCSGG 2 cut(s) 456, 538
BcoDI GTCTC 1 cut(s) 184
BfaI CTAG 3 cut(s) 389, 852, 1283
BfmI CTRYAG 5 cut(s) 415, 609, 903, 960, 1136
BfuAI ACCTGC 2 cut(s) 1042, 1102
BglII AGATCT 1 cut(s) 261
BisI GCNGC 5 cut(s) 231, 255, 1000, 1011, 1091
BlsI GCNGC 5 cut(s) 232, 256, 1001, 1012, 1092
Bme1390I CCNGG 3 cut(s) 456, 538, 809
Bme18I GGWCC 2 cut(s) 373, 747
BmgT120I GGNCC 3 cut(s) 373, 747, 913
BmiI GGNNCC 1 cut(s) 453
BmrFI CCNGG 3 cut(s) 456, 538, 809
BmsI GCATC 1 cut(s) 1089
BpiI GAAGAC 1 cut(s) 1073
BpuEI CTTGAG 1 cut(s) 1202
BpuMI CCSGG 2 cut(s) 456, 538
BsaHI GRCGYC 1 cut(s) 1040
BsaJI CCNNGG 1 cut(s) 537
BsaWI WCCGGW 2 cut(s) 316, 342
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 1 cut(s) 1012
Bse1I ACTGG 3 cut(s) 359, 923, 1065
BseBI CCWGG 1 cut(s) 809
BseDI CCNNGG 1 cut(s) 537
BseGI GGATG 3 cut(s) 197, 690, 1357
BseLI CCNNNNNNNGG 1 cut(s) 1012
BseMII CTCAG 2 cut(s) 107, 195
BseNI ACTGG 3 cut(s) 359, 923, 1065
BseRI GAGGAG 4 cut(s) 385, 621, 851, 923
BseXI GCAGC 5 cut(s) 241, 242, 1011, 1022, 1077
BshFI GGCC 2 cut(s) 915, 1142
BsiSI CCGG 5 cut(s) 63, 317, 343, 455, 537
BslFI GGGAC 1 cut(s) 203
BslI CCNNNNNNNGG 1 cut(s) 1012
BsmAI GTCTC 1 cut(s) 184
BsmFI GGGAC 1 cut(s) 203
BsmI GAATGC 3 cut(s) 33, 270, 1165
BsnI GGCC 2 cut(s) 915, 1142
Bsp143I GATC 7 cut(s) 96, 193, 261, 379, 409, 715, 1119
BspACI CCGC 4 cut(s) 155, 405, 882, 1013
BspANI GGCC 2 cut(s) 915, 1142
BspCNI CTCAG 2 cut(s) 106, 194
BspHI TCATGA 1 cut(s) 244
BspLI GGNNCC 1 cut(s) 453
BspMAI CTGCAG 3 cut(s) 613, 907, 964
BspMI ACCTGC 2 cut(s) 1042, 1102
BspPI GGATC 2 cut(s) 188, 404
BsrBI CCGCTC 1 cut(s) 405
BsrI ACTGG 3 cut(s) 359, 923, 1065
BssECI CCNNGG 1 cut(s) 537
BssMI GATC 7 cut(s) 96, 193, 261, 379, 409, 715, 1119
BssNI GRCGYC 1 cut(s) 1040
BssSI CACGAG 3 cut(s) 22, 208, 363
Bst2BI CACGAG 3 cut(s) 22, 208, 363
Bst2UI CCWGG 1 cut(s) 809
Bst4CI ACNGT 2 cut(s) 138, 419
Bst6I CTCTTC 1 cut(s) 403
BstACI GRCGYC 1 cut(s) 1040
BstAPI GCANNNNNTGC 3 cut(s) 558, 1099, 1225
BstC8I GCNNGC 2 cut(s) 483, 903
BstDEI CTNAG 5 cut(s) 93, 181, 278, 284, 815
BstF5I GGATG 3 cut(s) 197, 690, 1357
BstHHI GCGC 1 cut(s) 977
BstKTI GATC 7 cut(s) 99, 196, 264, 382, 412, 718, 1122
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 7 cut(s) 96, 193, 261, 379, 409, 715, 1119
BstMWI GCNNNNNNNGC 7 cut(s) 558, 1016, 1099, 1108, 1225, 1292, 1301
BstNI CCWGG 1 cut(s) 809
BstNSI RCATGY 1 cut(s) 489
BstSCI CCNGG 3 cut(s) 454, 536, 807
BstSFI CTRYAG 5 cut(s) 415, 609, 903, 960, 1136
BstV1I GCAGC 5 cut(s) 241, 242, 1011, 1022, 1077
BstV2I GAAGAC 1 cut(s) 1073
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BsuRI GGCC 2 cut(s) 915, 1142
BtgZI GCGATG 1 cut(s) 1262
BtsCI GGATG 3 cut(s) 197, 690, 1357
BtsI GCAGTG 2 cut(s) 127, 618
BtsIMutI CAGTG 4 cut(s) 127, 424, 618, 916
BveI ACCTGC 2 cut(s) 1042, 1102
Cac8I GCNNGC 2 cut(s) 483, 903
CciI TCATGA 1 cut(s) 244
CfoI GCGC 1 cut(s) 977
Cfr13I GGNCC 3 cut(s) 373, 747, 913
CseI GACGC 2 cut(s) 1029, 1340
CsiI ACCWGGT 1 cut(s) 807
Csp6I GTAC 1 cut(s) 352
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 5 cut(s) 93, 181, 278, 284, 815
DpnI GATC 7 cut(s) 98, 195, 263, 381, 411, 717, 1121
DpnII GATC 7 cut(s) 96, 193, 261, 379, 409, 715, 1119
Eam1104I CTCTTC 1 cut(s) 403
EarI CTCTTC 1 cut(s) 403
Eco32I GATATC 2 cut(s) 1153, 1204
Eco47I GGWCC 2 cut(s) 373, 747
EcoRII CCWGG 1 cut(s) 807
EcoRV GATATC 2 cut(s) 1153, 1204
FalI AAGNNNNNCTT 2 cut(s) 1297, 1329
FaqI GGGAC 1 cut(s) 203
FauI CCCGC 1 cut(s) 875
FauNDI CATATG 1 cut(s) 104
FbaI TGATCA 1 cut(s) 379
FblI GTMKAC 1 cut(s) 1135
Fnu4HI GCNGC 5 cut(s) 231, 255, 1000, 1011, 1091
FokI GGATG 3 cut(s) 184, 697, 1364
Fsp4HI GCNGC 5 cut(s) 231, 255, 1000, 1011, 1091
FspBI CTAG 3 cut(s) 389, 852, 1283
GlaI GCGC 1 cut(s) 976
GluI GCNGC 5 cut(s) 231, 255, 1000, 1011, 1091
HaeIII GGCC 2 cut(s) 915, 1142
HapII CCGG 5 cut(s) 63, 317, 343, 455, 537
HgaI GACGC 2 cut(s) 1029, 1340
HhaI GCGC 1 cut(s) 977
Hin1I GRCGYC 1 cut(s) 1040
Hin6I GCGC 1 cut(s) 975
HinP1I GCGC 1 cut(s) 975
HincII GTYRAC 1 cut(s) 890
HindII GTYRAC 1 cut(s) 890
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 5 cut(s) 8, 66, 121, 886, 989
HpaII CCGG 5 cut(s) 63, 317, 343, 455, 537
HphI GGTGA 6 cut(s) 17, 95, 419, 556, 695, 751
Hpy166II GTNNAC 5 cut(s) 547, 651, 890, 934, 1136
Hpy188I TCNGA 6 cut(s) 39, 96, 184, 715, 957, 1007
Hpy188III TCNNGA 5 cut(s) 148, 245, 986, 1117, 1156
Hpy8I GTNNAC 5 cut(s) 547, 651, 890, 934, 1136
HpyAV CCTTC 4 cut(s) 24, 436, 992, 1323
HpyCH4III ACNGT 2 cut(s) 138, 419
HpyCH4IV ACGT 1 cut(s) 694
HpyF10VI GCNNNNNNNGC 7 cut(s) 558, 1016, 1099, 1108, 1225, 1292, 1301
HpyF3I CTNAG 5 cut(s) 93, 181, 278, 284, 815
HpySE526I ACGT 1 cut(s) 694
Hsp92I GRCGYC 1 cut(s) 1040
HspAI GCGC 1 cut(s) 975
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 7 cut(s) 96, 193, 261, 379, 409, 715, 1119
Lsp1109I GCAGC 5 cut(s) 241, 242, 1011, 1022, 1077
LweI GCATC 1 cut(s) 1089
MabI ACCWGGT 1 cut(s) 807
MaeI CTAG 3 cut(s) 389, 852, 1283
MaeII ACGT 1 cut(s) 694
MaeIII GTNAC 4 cut(s) 5, 498, 1234, 1370
MalI GATC 7 cut(s) 98, 195, 263, 381, 411, 717, 1121
MbiI CCGCTC 1 cut(s) 405
MboI GATC 7 cut(s) 96, 193, 261, 379, 409, 715, 1119
MboII GAAGA 4 cut(s) 390, 442, 587, 1073
MfeI CAATTG 2 cut(s) 115, 160
MflI RGATCY 1 cut(s) 261
MluCI AATT 8 cut(s) 115, 160, 493, 670, 709, 765, 784, 1194
MlyI GAGTC 2 cut(s) 2, 895
MmeI TCCRAC 2 cut(s) 669, 813
MseI TTAA 5 cut(s) 675, 768, 926, 1193, 1359
MslI CAYNNNNRTG 1 cut(s) 1339
MspA1I CMGCKG 2 cut(s) 309, 1013
MspI CCGG 5 cut(s) 63, 317, 343, 455, 537
MspR9I CCNGG 3 cut(s) 456, 538, 809
MunI CAATTG 2 cut(s) 115, 160
Mva1269I GAATGC 3 cut(s) 33, 270, 1165
MvaI CCWGG 1 cut(s) 809
MwoI GCNNNNNNNGC 7 cut(s) 558, 1016, 1099, 1108, 1225, 1292, 1301
NciI CCSGG 2 cut(s) 456, 538
NdeI CATATG 1 cut(s) 104
NdeII GATC 7 cut(s) 96, 193, 261, 379, 409, 715, 1119
NlaIV GGNNCC 1 cut(s) 453
NmuCI GTSAC 2 cut(s) 5, 1234
NspI RCATGY 1 cut(s) 489
PagI TCATGA 1 cut(s) 244
PctI GAATGC 3 cut(s) 33, 270, 1165
PfeI GAWTC 3 cut(s) 66, 121, 989
PkrI GCNGC 5 cut(s) 232, 256, 1001, 1012, 1092
PleI GAGTC 2 cut(s) 2, 894
PpsI GAGTC 2 cut(s) 2, 894
Psp6I CCWGG 1 cut(s) 807
PspGI CCWGG 1 cut(s) 807
PspN4I GGNNCC 1 cut(s) 453
PspPI GGNCC 3 cut(s) 373, 747, 913
PstI CTGCAG 3 cut(s) 613, 907, 964
PsuI RGATCY 1 cut(s) 261
PvuII CAGCTG 1 cut(s) 309
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
RseI CAYNNNNRTG 1 cut(s) 1339
SaqAI TTAA 5 cut(s) 675, 768, 926, 1193, 1359
SatI GCNGC 5 cut(s) 231, 255, 1000, 1011, 1091
Sau3AI GATC 7 cut(s) 96, 193, 261, 379, 409, 715, 1119
Sau96I GGNCC 3 cut(s) 373, 747, 913
SchI GAGTC 2 cut(s) 2, 895
ScrFI CCNGG 3 cut(s) 456, 538, 809
SexAI ACCWGGT 1 cut(s) 807
SfaNI GCATC 1 cut(s) 1089
SfcI CTRYAG 5 cut(s) 415, 609, 903, 960, 1136
SinI GGWCC 2 cut(s) 373, 747
SmiMI CAYNNNNRTG 1 cut(s) 1339
SmlI CTYRAG 1 cut(s) 1181
SmoI CTYRAG 1 cut(s) 1181
Sse9I AATT 8 cut(s) 115, 160, 493, 670, 709, 765, 784, 1194
SsiI CCGC 4 cut(s) 155, 405, 882, 1013
SspI AATATT 1 cut(s) 1291
SspMI CTAG 3 cut(s) 389, 852, 1283
StyD4I CCNGG 3 cut(s) 454, 536, 807
TaaI ACNGT 2 cut(s) 138, 419
TaiI ACGT 1 cut(s) 697
TaqI TCGA 6 cut(s) 401, 524, 793, 844, 1122, 1365
TaqII GACCGA 1 cut(s) 361
TasI AATT 8 cut(s) 115, 160, 493, 670, 709, 765, 784, 1194
TfiI GAWTC 3 cut(s) 66, 121, 989
Tru1I TTAA 5 cut(s) 675, 768, 926, 1193, 1359
Tru9I TTAA 5 cut(s) 675, 768, 926, 1193, 1359
TscAI CASTG 4 cut(s) 134, 424, 618, 923
TseFI GTSAC 2 cut(s) 5, 1234
TseI GCWGC 5 cut(s) 230, 254, 999, 1010, 1090
Tsp45I GTSAC 2 cut(s) 5, 1234
TspDTI ATGAA 4 cut(s) 615, 629, 777, 1314
TspRI CASTG 4 cut(s) 134, 424, 618, 923
VpaK11BI GGWCC 2 cut(s) 373, 747
XceI RCATGY 1 cut(s) 489
XmiI GTMKAC 1 cut(s) 1135
XspI CTAG 3 cut(s) 389, 852, 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.