Rh5CG432400

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0002756
Physical Location & Seq
Forward (+)
59880924 .. 59884964
4041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG432400.1

Sequence Viewer

Length: 1200 bp
ATGACCCAGTCGCCTTCCGACTCACTGCTTGATCATCATGCTCGTCCTCATCTTGTGCCCGAAATCCCAATTGAGATCGTAAGCGACGAAGAGATGGCTCTGCTCGAAGCCGCCTTGCTCTCCGCTGGCTCTCTAATCCCTTCCACAGTGAGAGCATTCCCTTCATTTCGATCCTCTCACTTCCCTTCCAATGTCAGGTCTATTCAGTCCATCACCCTCGTTTCCAAGAAGAGGTTTTCGGGTTGCTCCCAACCCGATATTGAGGATTCGGGTTTAGGGTTCAACACCACCCAGAAGAAGACCCGGTTGGCTGAGTCGTTTTTGCACCGCTTCAGGAGGAAGAAGGGCTTGTGCGTCACCGATTTTACTTCCGCGGAATGGTGTGAAAAACAGACGGAGTTTGAGCTCCTCGGCGGCAAGAAAAAAATTAATAAGGCTATGAAAGCTGGTAGTAAGCGCCATGCAAAACTCGAAGAAGAGGTTCTAACAAGAGTTAAAGTTGATATCAAATCAGACGAAGATAAATTTGCTCTGAAGCTTTTGAATTGTATTACCGGCATAAATCAATTACAATTTGAAGGATTGACTCGAGAGCTGCCTCTCATAGGTTTTGTAGAAGGTGTATGGATGGTGGGAGTGATTGATGAGGTTCAAATGCCTGCAACAGAAACTCAGAGAAATCCATTATTAGTTGACACAAAGACTCGTGTGAAAGATACACTTCCTGCTGAACCACAACGAAGGAATGGAAGGCTTCAATTGATGTGCTACAAGTATATGTGGGACAATTTAGTTTCTTCTGAATTCCCCTCCGACCAATTCTTTGATTATTTTTCCTTAAATCCTGATTGTGACTTATCTGAAGAAATCAGGGAGGTCACAGCTAAATCAGGGTGTCCAGCAAAGACCCTTGATGACGTAGTGAGATACTATAGAAATACCTGGAGCATGCTACCCCCTGCTCATGACCAACTTTTATTGAGATACGAGTTACAAAAAGACCATTCACTAATCGGCGAAGATAAATTTGCATATGACTCTGATTGGGTTGAGAAGCAAATTCAGTTTTCTCTCGAGTTCTGGTTAGGAGAGCGAGAAGCCAGTTACACTCCTGAGGAAGAGCGATGGAAATGCAACTTCTGTCAATTTGCTTCCACATGTCCTGCTAAAAGTGTAACCTGCAGCAACCTGCCGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

399

Amino Acids

45.6

Weight (kDa)

5.76

Isoelectric Point (pI)

48.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 106 - 223 1.1e-36 Exonuclease V - a 5' deoxyribonuclease
PDDEXK_1 PF12705 193 - 388 3.3e-06 PD-(D/E)XK nuclease superfamily
Exo5 PF09810 223 - 291 6.4e-08 Exonuclease V - a 5' deoxyribonuclease
Exo5 PF09810 321 - 387 1.8e-11 Exonuclease V - a 5' deoxyribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1187
AccII CGCG 1 cut(s) 374
AciI CCGC 6 cut(s) 111, 123, 328, 372, 374, 414
AclWI GGATC 1 cut(s) 165
AcsI RAATTY 4 cut(s) 524, 803, 1025, 1059
AcuI CTGAAG 3 cut(s) 316, 554, 882
AfiI CCNNNNNNNGG 4 cut(s) 195, 231, 378, 605
AflIII ACRYGT 1 cut(s) 1157
AgsI TTSAA 5 cut(s) 283, 544, 578, 653, 758
AjnI CCWGG 1 cut(s) 941
AjuI GAANNNNNNNTTGG 2 cut(s) 290, 322
AluBI AGCT 5 cut(s) 406, 446, 538, 595, 884
AluI AGCT 5 cut(s) 406, 446, 538, 595, 884
Alw21I GWGCWC 1 cut(s) 408
AlwI GGATC 1 cut(s) 165
Ama87I CYCGRG 2 cut(s) 588, 1073
ApeKI GCWGC 2 cut(s) 595, 1182
ApoI RAATTY 4 cut(s) 524, 803, 1025, 1059
ArsI GACNNNNNNTTYG 2 cut(s) 806, 838
AseI ATTAAT 1 cut(s) 429
Asp700I GAANNNNTTC 1 cut(s) 480
AspLEI GCGC 1 cut(s) 459
AsuC2I CCSGG 1 cut(s) 304
AsuHPI GGTGA 2 cut(s) 205, 349
AvaI CYCGRG 2 cut(s) 588, 1073
AxyI CCTNAGG 1 cut(s) 1113
BaeGI GKGCMC 1 cut(s) 60
BanII GRGCYC 1 cut(s) 408
BauI CACGAG 1 cut(s) 705
BbsI GAAGAC 1 cut(s) 305
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 2 cut(s) 582, 1194
BccI CCATC 4 cut(s) 88, 218, 622, 1119
BcgI CGANNNNNNTGC 2 cut(s) 1113, 1147
BciT130I CCWGG 1 cut(s) 943
BclI TGATCA 1 cut(s) 31
BcnI CCSGG 1 cut(s) 304
BfmI CTRYAG 2 cut(s) 931, 1180
BfoI RGCGCY 1 cut(s) 460
BfuAI ACCTGC 1 cut(s) 1187
BisI GCNGC 4 cut(s) 111, 415, 596, 1183
BlsI GCNGC 4 cut(s) 112, 416, 597, 1184
Bme1390I CCNGG 2 cut(s) 304, 943
BmeT110I CYCGRG 2 cut(s) 588, 1073
BmrFI CCNGG 2 cut(s) 304, 943
BpiI GAAGAC 1 cut(s) 305
BpmI CTGGAG 1 cut(s) 964
BpuMI CCSGG 1 cut(s) 304
BsaJI CCNNGG 2 cut(s) 372, 409
Bsc4I CCNNNNNNNGG 4 cut(s) 195, 231, 378, 605
Bse118I RCCGGY 1 cut(s) 554
Bse1I ACTGG 2 cut(s) 7, 1101
Bse21I CCTNAGG 1 cut(s) 1113
BseBI CCWGG 1 cut(s) 943
BseDI CCNNGG 2 cut(s) 372, 409
BseGI GGATG 1 cut(s) 633
BseLI CCNNNNNNNGG 4 cut(s) 195, 231, 378, 605
BseMII CTCAG 3 cut(s) 303, 686, 1104
BseNI ACTGG 2 cut(s) 7, 1101
BseRI GAGGAG 1 cut(s) 398
BseSI GKGCMC 1 cut(s) 60
BseXI GCAGC 2 cut(s) 582, 1194
Bsh1236I CGCG 1 cut(s) 374
BsiHKAI GWGCWC 1 cut(s) 408
BsiHKCI CYCGRG 2 cut(s) 588, 1073
BsiSI CCGG 2 cut(s) 304, 555
BslFI GGGAC 1 cut(s) 797
BslI CCNNNNNNNGG 4 cut(s) 195, 231, 378, 605
BsmFI GGGAC 1 cut(s) 797
BsmI GAATGC 1 cut(s) 155
BsoBI CYCGRG 2 cut(s) 588, 1073
Bsp1286I GDGCHC 2 cut(s) 60, 408
Bsp143I GATC 3 cut(s) 31, 75, 170
BspACI CCGC 6 cut(s) 111, 123, 328, 372, 374, 414
BspCNI CTCAG 3 cut(s) 304, 685, 1105
BspFNI CGCG 1 cut(s) 374
BspHI TCATGA 1 cut(s) 964
BspMAI CTGCAG 1 cut(s) 1184
BspMI ACCTGC 1 cut(s) 1187
BspPI GGATC 1 cut(s) 165
BspQI GCTCTTC 1 cut(s) 1113
BsrFI RCCGGY 1 cut(s) 554
BsrI ACTGG 2 cut(s) 7, 1101
BssAI RCCGGY 1 cut(s) 554
BssECI CCNNGG 2 cut(s) 372, 409
BssMI GATC 3 cut(s) 31, 75, 170
BssSI CACGAG 1 cut(s) 705
Bst2BI CACGAG 1 cut(s) 705
Bst2UI CCWGG 1 cut(s) 943
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 4 cut(s) 84, 224, 471, 1113
BstC8I GCNNGC 3 cut(s) 127, 660, 950
BstDEI CTNAG 4 cut(s) 312, 672, 1113, 1197
BstDSI CCRYGG 1 cut(s) 372
BstENI CCTNNNNNAGG 1 cut(s) 603
BstF5I GGATG 1 cut(s) 633
BstFNI CGCG 1 cut(s) 374
BstH2I RGCGCY 1 cut(s) 460
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 3 cut(s) 34, 78, 173
BstMBI GATC 3 cut(s) 31, 75, 170
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstNI CCWGG 1 cut(s) 943
BstNSI RCATGY 2 cut(s) 952, 1161
BstSCI CCNGG 2 cut(s) 302, 941
BstSFI CTRYAG 2 cut(s) 931, 1180
BstSLI GKGCMC 1 cut(s) 60
BstUI CGCG 1 cut(s) 374
BstV1I GCAGC 2 cut(s) 582, 1194
BstV2I GAAGAC 1 cut(s) 305
Bsu36I CCTNAGG 1 cut(s) 1113
BtgI CCRYGG 1 cut(s) 372
BtgZI GCGATG 1 cut(s) 1138
BtsCI GGATG 1 cut(s) 633
BtsI GCAGTG 1 cut(s) 23
BtsIMutI CAGTG 2 cut(s) 23, 153
BveI ACCTGC 1 cut(s) 1187
Cac8I GCNNGC 3 cut(s) 127, 660, 950
CciI TCATGA 1 cut(s) 964
CfoI GCGC 1 cut(s) 459
Cfr10I RCCGGY 1 cut(s) 554
Cfr42I CCGCGG 1 cut(s) 375
CseI GACGC 1 cut(s) 343
CviAII CATG 5 cut(s) 38, 461, 949, 965, 1158
DdeI CTNAG 4 cut(s) 312, 672, 1113, 1197
DpnI GATC 3 cut(s) 33, 77, 172
DpnII GATC 3 cut(s) 31, 75, 170
Eam1104I CTCTTC 4 cut(s) 84, 224, 471, 1113
EarI CTCTTC 4 cut(s) 84, 224, 471, 1113
Ecl136II GAGCTC 1 cut(s) 406
Eco24I GRGCYC 1 cut(s) 408
Eco32I GATATC 1 cut(s) 505
Eco53kI GAGCTC 1 cut(s) 406
Eco57I CTGAAG 3 cut(s) 316, 554, 882
Eco81I CCTNAGG 1 cut(s) 1113
Eco88I CYCGRG 2 cut(s) 588, 1073
EcoICRI GAGCTC 1 cut(s) 406
EcoNI CCTNNNNNAGG 1 cut(s) 603
EcoRI GAATTC 1 cut(s) 803
EcoRII CCWGG 1 cut(s) 941
EcoRV GATATC 1 cut(s) 505
EcoT38I GRGCYC 1 cut(s) 408
FaeI CATG 5 cut(s) 41, 464, 952, 968, 1161
FalI AAGNNNNNCTT 4 cut(s) 332, 364, 705, 737
FaqI GGGAC 1 cut(s) 797
FatI CATG 5 cut(s) 37, 460, 948, 964, 1157
FauNDI CATATG 1 cut(s) 1033
FbaI TGATCA 1 cut(s) 31
Fnu4HI GCNGC 4 cut(s) 111, 415, 596, 1183
FokI GGATG 1 cut(s) 640
FriOI GRGCYC 1 cut(s) 408
Fsp4HI GCNGC 4 cut(s) 111, 415, 596, 1183
GlaI GCGC 1 cut(s) 458
GluI GCNGC 4 cut(s) 111, 415, 596, 1183
GsuI CTGGAG 1 cut(s) 964
HaeII RGCGCY 1 cut(s) 460
HapII CCGG 2 cut(s) 304, 555
HgaI GACGC 1 cut(s) 343
HhaI GCGC 1 cut(s) 459
Hin1II CATG 5 cut(s) 41, 464, 952, 968, 1161
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HincII GTYRAC 1 cut(s) 694
HindII GTYRAC 1 cut(s) 694
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 6 cut(s) 20, 266, 314, 586, 703, 1037
HpaII CCGG 2 cut(s) 304, 555
HphI GGTGA 2 cut(s) 205, 349
Hpy166II GTNNAC 1 cut(s) 694
Hpy188I TCNGA 8 cut(s) 19, 514, 534, 675, 802, 814, 862, 1042
Hpy188III TCNNGA 6 cut(s) 334, 590, 845, 965, 1073, 1112
Hpy8I GTNNAC 1 cut(s) 694
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 9 cut(s) 24, 150, 171, 195, 337, 572, 611, 735, 744
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 1 cut(s) 918
HpyCH4V TGCA 6 cut(s) 325, 464, 662, 1031, 1134, 1182
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 4 cut(s) 312, 672, 1113, 1197
HpySE526I ACGT 1 cut(s) 918
Hsp92II CATG 5 cut(s) 41, 464, 952, 968, 1161
HspAI GCGC 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 31
KspI CCGCGG 1 cut(s) 375
Kzo9I GATC 3 cut(s) 31, 75, 170
LguI GCTCTTC 1 cut(s) 1113
LmnI GCTCC 3 cut(s) 251, 411, 945
Lsp1109I GCAGC 2 cut(s) 582, 1194
MaeII ACGT 1 cut(s) 918
MaeIII GTNAC 6 cut(s) 355, 851, 877, 990, 1103, 1174
MalI GATC 3 cut(s) 33, 77, 172
MboI GATC 3 cut(s) 31, 75, 170
MfeI CAATTG 2 cut(s) 69, 758
MhlI GDGCHC 2 cut(s) 60, 408
MlyI GAGTC 5 cut(s) 14, 323, 580, 697, 1031
MmeI TCCRAC 2 cut(s) 42, 837
MroXI GAANNNNTTC 1 cut(s) 480
MseI TTAA 3 cut(s) 429, 495, 839
MspA1I CMGCKG 2 cut(s) 125, 374
MspI CCGG 2 cut(s) 304, 555
MspR9I CCNGG 2 cut(s) 304, 943
MunI CAATTG 2 cut(s) 69, 758
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 1 cut(s) 943
MvnI CGCG 1 cut(s) 374
MwoI GCNNNNNNNGC 1 cut(s) 443
NciI CCSGG 1 cut(s) 304
NdeI CATATG 1 cut(s) 1033
NdeII GATC 3 cut(s) 31, 75, 170
NlaIII CATG 5 cut(s) 41, 464, 952, 968, 1161
NmeAIII GCCGAG 1 cut(s) 390
NmuCI GTSAC 3 cut(s) 355, 851, 877
NspI RCATGY 2 cut(s) 952, 1161
PaeI GCATGC 1 cut(s) 952
PaeR7I CTCGAG 2 cut(s) 588, 1073
PagI TCATGA 1 cut(s) 964
PciI ACATGT 1 cut(s) 1157
PciSI GCTCTTC 1 cut(s) 1113
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PctI GAATGC 1 cut(s) 155
PdmI GAANNNNTTC 1 cut(s) 480
PfeI GAWTC 1 cut(s) 266
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 4 cut(s) 112, 416, 597, 1184
PleI GAGTC 5 cut(s) 14, 322, 580, 697, 1031
PpsI GAGTC 5 cut(s) 14, 322, 580, 697, 1031
PscI ACATGT 1 cut(s) 1157
PshBI ATTAAT 1 cut(s) 429
Psp124BI GAGCTC 1 cut(s) 408
Psp6I CCWGG 1 cut(s) 941
PspGI CCWGG 1 cut(s) 941
PstI CTGCAG 1 cut(s) 1184
PsyI GACNNNGTC 1 cut(s) 7
SacI GAGCTC 1 cut(s) 408
SacII CCGCGG 1 cut(s) 375
SapI GCTCTTC 1 cut(s) 1113
SaqAI TTAA 3 cut(s) 429, 495, 839
SatI GCNGC 4 cut(s) 111, 415, 596, 1183
Sau3AI GATC 3 cut(s) 31, 75, 170
SchI GAGTC 5 cut(s) 14, 323, 580, 697, 1031
ScrFI CCNGG 2 cut(s) 304, 943
SduI GDGCHC 2 cut(s) 60, 408
SfcI CTRYAG 2 cut(s) 931, 1180
Sfr274I CTCGAG 2 cut(s) 588, 1073
Sfr303I CCGCGG 1 cut(s) 375
SgrBI CCGCGG 1 cut(s) 375
SlaI CTCGAG 2 cut(s) 588, 1073
SmlI CTYRAG 2 cut(s) 588, 1073
SmoI CTYRAG 2 cut(s) 588, 1073
SphI GCATGC 1 cut(s) 952
SsiI CCGC 6 cut(s) 111, 123, 328, 372, 374, 414
SstI GAGCTC 1 cut(s) 408
StyD4I CCNGG 2 cut(s) 302, 941
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 1 cut(s) 921
TaqI TCGA 5 cut(s) 105, 169, 471, 589, 1074
TauI GCSGC 2 cut(s) 113, 417
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 3 cut(s) 429, 495, 839
Tru9I TTAA 3 cut(s) 429, 495, 839
TscAI CASTG 2 cut(s) 30, 153
TseFI GTSAC 3 cut(s) 355, 851, 877
TseI GCWGC 2 cut(s) 595, 1182
Tsp45I GTSAC 3 cut(s) 355, 851, 877
TspDTI ATGAA 2 cut(s) 153, 455
TspGWI ACGGA 1 cut(s) 410
TspRI CASTG 2 cut(s) 30, 153
Tth111I GACNNNGTC 1 cut(s) 7
VspI ATTAAT 1 cut(s) 429
XagI CCTNNNNNAGG 1 cut(s) 603
XapI RAATTY 4 cut(s) 524, 803, 1025, 1059
XceI RCATGY 2 cut(s) 952, 1161
XhoI CTCGAG 2 cut(s) 588, 1073
XmnI GAANNNNTTC 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.