Rroxscaffold_1G00020200

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002756
Physical Location & Seq
Reverse (-)
24543090 .. 24547223
4134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00020200.1

Sequence Viewer

Length: 1458 bp
ATGTGCAACAGAGTACAGGACGTTTTGAATAACTTGCCCCATAAGGCGTGTGCCGCTGCCAACGGTCAATTCCGATCCAAAATGACCCAGTCGCCTTCAGACTCACTCCTCGATCATCATCCTCGACCTCATCTTGTGCCCGAAATCCCAATTGAGATCGTCAGCGACGAAGAGATGGCTCTGCTCGAAGCCGCCTTGCTCTCCGCTGGCTCTCTAATCCCTTCCACAGTGAGAGCATTCCCTTCATTTCGATCCTCTCACTTCCCTTCCAATGCCAGGTCTATTCAGTCCATCACCCTCGTTTCCAAGAAGAGGTTTTCGGGTTGCTCCCAACCCGATATTGAGGATTCGGGTTTAGGGTTCAACACTACCCAGAAGAAGACCCGGTTGGCTGAGTCGTTTTTGCACCGCTTCAGGAGGAAGAAGGGCTTGTGCGTCACCGATTTTACTTCCGCGGTATGGAAAATTCTTCGCTTTGGGACTCTTTGCTTAGTCTTTTGGGAATTTGGGTTTGGGTTTGCGCTCGTCAATTTTGAAACTTGTGTGTTTGATTGTGAATCTTGCTTTGTGTTTGATTGTGAATCCTGTGGGACTGGTTTGGATGGCTTTGGTGAACTTTTGAGATTTATTGAGGAATGGTGTGAAAAACAGATGGAGTTTGAGCTCCTCGGCGGCAAGAAAAAAATTAGTAAGGCTATGAAAGCTGGTAGTAAGCGCCATGCAAAACTCGAAGAAGAGGTTCTAACAAGAGTTAAAGTTGATATCAAATCAGACGAAGATAAATTTGCTCTGAAGCTTTTGAATTGTATTACCGGCATAAATCAATTACAATTTGAAGGATTGACTCGAGAGCTACCTCTCATAGGTTTTGTAGAAGGTGTATGGATGGTGGGAGTGATTGATGAGGTTCAATTTCCTGCAACAGAAACCCAGAGAAATCCATTATTAGTTGACACAAAGACTCGTGTGAAAGATACACTTCCTGCTGAACCACAACGAAGGAATGGAAGGCTTCAATTGATGTGCTACAAGTATATGTGGGACAATTTAGTTACTTCTGAATTCCCCTCCGACCAATTCTTTGATTATTTTTCCTTAAATCCTGATTGTGACTTATCTGAAGAAATCAGGGAGGTCACAGCTAAATCAGGGTGTCCAGCAAAGACCCTTGATGACGTAGTGAGATACTATAGAAATACCTGGAGCATGCTACCCCCTGCTCATGACCAACTTTTATTGAGATACGAGTTACAAAAAGACCATTCACTAATCGGCGAAGATAAATTTGCATACAACTCTGATTGGGTTGAGAAGCAAATTCAGTTTTCTCTCGAGTTCTGGTTAGGAGAGCGAGAAGCCAGTTACACTCCTGAGGAAGAGCGATGGAAATGCAACTTCTGTCAATTTGCTTCCACATGTCCTGCTAAAAGTGTAACCTGCAGCAACCTGCCGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

485

Amino Acids

55.34

Weight (kDa)

5.66

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 212 - 309 5.4e-30 Exonuclease V - a 5' deoxyribonuclease
PDDEXK_1 PF12705 279 - 474 4.7e-06 PD-(D/E)XK nuclease superfamily
Exo5 PF09810 310 - 377 8.7e-08 Exonuclease V - a 5' deoxyribonuclease
Exo5 PF09810 407 - 473 3.7e-11 Exonuclease V - a 5' deoxyribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1445
AccII CGCG 1 cut(s) 455
AciI CCGC 7 cut(s) 54, 192, 204, 409, 453, 455, 672
AclWI GGATC 2 cut(s) 69, 246
AcsI RAATTY 6 cut(s) 465, 503, 782, 1061, 1283, 1317
AcuI CTGAAG 4 cut(s) 81, 397, 812, 1140
AfaI GTAC 1 cut(s) 15
AfiI CCNNNNNNNGG 4 cut(s) 276, 312, 459, 863
AflIII ACRYGT 1 cut(s) 1415
AgsI TTSAA 7 cut(s) 28, 364, 536, 802, 836, 911, 1016
AjnI CCWGG 2 cut(s) 275, 1199
AjuI GAANNNNNNNTTGG 6 cut(s) 53, 85, 371, 403, 495, 527
AluBI AGCT 5 cut(s) 664, 704, 796, 853, 1142
AluI AGCT 5 cut(s) 664, 704, 796, 853, 1142
Alw21I GWGCWC 1 cut(s) 666
AlwI GGATC 2 cut(s) 69, 246
Ama87I CYCGRG 2 cut(s) 846, 1331
ApeKI GCWGC 2 cut(s) 56, 1440
ApoI RAATTY 6 cut(s) 465, 503, 782, 1061, 1283, 1317
ArsI GACNNNNNNTTYG 2 cut(s) 1064, 1096
Asp700I GAANNNNTTC 1 cut(s) 738
AspLEI GCGC 2 cut(s) 523, 717
AsuC2I CCSGG 1 cut(s) 385
AsuHPI GGTGA 3 cut(s) 286, 430, 623
AvaI CYCGRG 2 cut(s) 846, 1331
AxyI CCTNAGG 1 cut(s) 1371
BaeGI GKGCMC 1 cut(s) 141
BanII GRGCYC 1 cut(s) 666
BauI CACGAG 1 cut(s) 963
BbsI GAAGAC 1 cut(s) 386
Bbv12I GWGCWC 1 cut(s) 666
BbvI GCAGC 2 cut(s) 43, 1452
BccI CCATC 6 cut(s) 169, 299, 596, 646, 880, 1377
BcgI CGANNNNNNTGC 2 cut(s) 1371, 1405
BciT130I CCWGG 2 cut(s) 277, 1201
BcnI CCSGG 1 cut(s) 385
BfmI CTRYAG 2 cut(s) 1189, 1438
BfoI RGCGCY 1 cut(s) 718
BfuAI ACCTGC 1 cut(s) 1445
BisI GCNGC 5 cut(s) 54, 57, 192, 673, 1441
BlsI GCNGC 5 cut(s) 55, 58, 193, 674, 1442
Bme1390I CCNGG 3 cut(s) 277, 385, 1201
BmeT110I CYCGRG 2 cut(s) 846, 1331
BmrFI CCNGG 3 cut(s) 277, 385, 1201
BmrI ACTGGG 1 cut(s) 82
BmuI ACTGGG 1 cut(s) 82
BpiI GAAGAC 1 cut(s) 386
BpmI CTGGAG 1 cut(s) 1222
BpuMI CCSGG 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 117
BsaJI CCNNGG 2 cut(s) 453, 667
Bsc4I CCNNNNNNNGG 4 cut(s) 276, 312, 459, 863
Bse118I RCCGGY 1 cut(s) 812
Bse1I ACTGG 3 cut(s) 88, 598, 1359
Bse21I CCTNAGG 1 cut(s) 1371
Bse8I GATNNNNATC 1 cut(s) 117
BseBI CCWGG 2 cut(s) 277, 1201
BseDI CCNNGG 2 cut(s) 453, 667
BseGI GGATG 3 cut(s) 118, 607, 891
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 4 cut(s) 276, 312, 459, 863
BseMII CTCAG 2 cut(s) 384, 1362
BseNI ACTGG 3 cut(s) 88, 598, 1359
BseRI GAGGAG 2 cut(s) 98, 656
BseSI GKGCMC 1 cut(s) 141
BseXI GCAGC 2 cut(s) 43, 1452
Bsh1236I CGCG 1 cut(s) 455
BsiHKAI GWGCWC 1 cut(s) 666
BsiHKCI CYCGRG 2 cut(s) 846, 1331
BsiSI CCGG 2 cut(s) 385, 813
BslFI GGGAC 3 cut(s) 493, 604, 1055
BslI CCNNNNNNNGG 4 cut(s) 276, 312, 459, 863
BsmFI GGGAC 3 cut(s) 493, 604, 1055
BsmI GAATGC 1 cut(s) 236
BsoBI CYCGRG 2 cut(s) 846, 1331
Bsp1286I GDGCHC 2 cut(s) 141, 666
Bsp143I GATC 4 cut(s) 74, 112, 156, 251
BspACI CCGC 7 cut(s) 54, 192, 204, 409, 453, 455, 672
BspCNI CTCAG 2 cut(s) 385, 1363
BspFNI CGCG 1 cut(s) 455
BspHI TCATGA 1 cut(s) 1222
BspMAI CTGCAG 1 cut(s) 1442
BspMI ACCTGC 1 cut(s) 1445
BspPI GGATC 2 cut(s) 69, 246
BspQI GCTCTTC 1 cut(s) 1371
BsrFI RCCGGY 1 cut(s) 812
BsrI ACTGG 3 cut(s) 88, 598, 1359
BssAI RCCGGY 1 cut(s) 812
BssECI CCNNGG 2 cut(s) 453, 667
BssMI GATC 4 cut(s) 74, 112, 156, 251
BssSI CACGAG 1 cut(s) 963
Bst2BI CACGAG 1 cut(s) 963
Bst2UI CCWGG 2 cut(s) 277, 1201
Bst4CI ACNGT 2 cut(s) 65, 229
Bst6I CTCTTC 4 cut(s) 165, 305, 729, 1371
BstC8I GCNNGC 2 cut(s) 208, 1208
BstDEI CTNAG 4 cut(s) 393, 490, 1371, 1455
BstDSI CCRYGG 1 cut(s) 453
BstENI CCTNNNNNAGG 1 cut(s) 861
BstF5I GGATG 3 cut(s) 118, 607, 891
BstFNI CGCG 1 cut(s) 455
BstH2I RGCGCY 1 cut(s) 718
BstHHI GCGC 2 cut(s) 523, 717
BstKTI GATC 4 cut(s) 77, 115, 159, 254
BstMBI GATC 4 cut(s) 74, 112, 156, 251
BstMWI GCNNNNNNNGC 2 cut(s) 53, 701
BstNI CCWGG 2 cut(s) 277, 1201
BstNSI RCATGY 2 cut(s) 1210, 1419
BstSCI CCNGG 3 cut(s) 275, 383, 1199
BstSFI CTRYAG 2 cut(s) 1189, 1438
BstSLI GKGCMC 1 cut(s) 141
BstUI CGCG 1 cut(s) 455
BstV1I GCAGC 2 cut(s) 43, 1452
BstV2I GAAGAC 1 cut(s) 386
Bsu36I CCTNAGG 1 cut(s) 1371
BtgI CCRYGG 1 cut(s) 453
BtgZI GCGATG 1 cut(s) 1396
BtsCI GGATG 3 cut(s) 118, 607, 891
BtsIMutI CAGTG 1 cut(s) 234
BveI ACCTGC 1 cut(s) 1445
Cac8I GCNNGC 2 cut(s) 208, 1208
CciI TCATGA 1 cut(s) 1222
CfoI GCGC 2 cut(s) 523, 717
Cfr10I RCCGGY 1 cut(s) 812
Cfr42I CCGCGG 1 cut(s) 456
CseI GACGC 1 cut(s) 424
Csp6I GTAC 1 cut(s) 14
CviAII CATG 4 cut(s) 719, 1207, 1223, 1416
CviQI GTAC 1 cut(s) 14
DdeI CTNAG 4 cut(s) 393, 490, 1371, 1455
DpnI GATC 4 cut(s) 76, 114, 158, 253
DpnII GATC 4 cut(s) 74, 112, 156, 251
Eam1104I CTCTTC 4 cut(s) 165, 305, 729, 1371
EarI CTCTTC 4 cut(s) 165, 305, 729, 1371
Ecl136II GAGCTC 1 cut(s) 664
Eco24I GRGCYC 1 cut(s) 666
Eco32I GATATC 1 cut(s) 763
Eco53kI GAGCTC 1 cut(s) 664
Eco57I CTGAAG 4 cut(s) 81, 397, 812, 1140
Eco81I CCTNAGG 1 cut(s) 1371
Eco88I CYCGRG 2 cut(s) 846, 1331
EcoICRI GAGCTC 1 cut(s) 664
EcoNI CCTNNNNNAGG 1 cut(s) 861
EcoRI GAATTC 1 cut(s) 1061
EcoRII CCWGG 2 cut(s) 275, 1199
EcoRV GATATC 1 cut(s) 763
EcoT38I GRGCYC 1 cut(s) 666
FaeI CATG 4 cut(s) 722, 1210, 1226, 1419
FalI AAGNNNNNCTT 4 cut(s) 413, 445, 963, 995
FaqI GGGAC 3 cut(s) 493, 604, 1055
FatI CATG 4 cut(s) 718, 1206, 1222, 1415
Fnu4HI GCNGC 5 cut(s) 54, 57, 192, 673, 1441
FokI GGATG 3 cut(s) 105, 614, 898
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 5 cut(s) 54, 57, 192, 673, 1441
GlaI GCGC 2 cut(s) 522, 716
GluI GCNGC 5 cut(s) 54, 57, 192, 673, 1441
GsuI CTGGAG 1 cut(s) 1222
HaeII RGCGCY 1 cut(s) 718
HapII CCGG 2 cut(s) 385, 813
HgaI GACGC 1 cut(s) 424
HhaI GCGC 2 cut(s) 523, 717
Hin1II CATG 4 cut(s) 722, 1210, 1226, 1419
Hin6I GCGC 2 cut(s) 521, 715
HinP1I GCGC 2 cut(s) 521, 715
HincII GTYRAC 1 cut(s) 952
HindII GTYRAC 1 cut(s) 952
HindIII AAGCTT 1 cut(s) 794
HinfI GANTC 8 cut(s) 101, 347, 395, 481, 557, 581, 844, 961
HpaII CCGG 2 cut(s) 385, 813
HphI GGTGA 3 cut(s) 286, 430, 623
Hpy166II GTNNAC 2 cut(s) 614, 952
Hpy188I TCNGA 8 cut(s) 74, 100, 772, 792, 1060, 1072, 1120, 1300
Hpy188III TCNNGA 6 cut(s) 415, 848, 1103, 1223, 1331, 1370
Hpy8I GTNNAC 2 cut(s) 614, 952
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 9 cut(s) 105, 231, 252, 276, 418, 830, 869, 993, 1002
HpyCH4III ACNGT 2 cut(s) 65, 229
HpyCH4IV ACGT 2 cut(s) 21, 1176
HpyCH4V TGCA 7 cut(s) 6, 406, 722, 920, 1289, 1392, 1440
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 701
HpyF3I CTNAG 4 cut(s) 393, 490, 1371, 1455
HpySE526I ACGT 2 cut(s) 21, 1176
Hsp92II CATG 4 cut(s) 722, 1210, 1226, 1419
HspAI GCGC 2 cut(s) 521, 715
KspI CCGCGG 1 cut(s) 456
Kzo9I GATC 4 cut(s) 74, 112, 156, 251
LguI GCTCTTC 1 cut(s) 1371
LmnI GCTCC 3 cut(s) 332, 669, 1203
Lsp1109I GCAGC 2 cut(s) 43, 1452
MaeII ACGT 2 cut(s) 21, 1176
MaeIII GTNAC 7 cut(s) 436, 1051, 1109, 1135, 1248, 1361, 1432
MalI GATC 4 cut(s) 76, 114, 158, 253
MboI GATC 4 cut(s) 74, 112, 156, 251
MfeI CAATTG 2 cut(s) 150, 1016
MhlI GDGCHC 2 cut(s) 141, 666
MlyI GAGTC 5 cut(s) 95, 404, 475, 838, 955
MmeI TCCRAC 1 cut(s) 1095
MroXI GAANNNNTTC 1 cut(s) 738
MseI TTAA 2 cut(s) 753, 1097
MspA1I CMGCKG 3 cut(s) 56, 206, 455
MspI CCGG 2 cut(s) 385, 813
MspR9I CCNGG 3 cut(s) 277, 385, 1201
MunI CAATTG 2 cut(s) 150, 1016
Mva1269I GAATGC 1 cut(s) 236
MvaI CCWGG 2 cut(s) 277, 1201
MvnI CGCG 1 cut(s) 455
MwoI GCNNNNNNNGC 2 cut(s) 53, 701
NciI CCSGG 1 cut(s) 385
NdeII GATC 4 cut(s) 74, 112, 156, 251
NlaIII CATG 4 cut(s) 722, 1210, 1226, 1419
NmeAIII GCCGAG 1 cut(s) 648
NmuCI GTSAC 3 cut(s) 436, 1109, 1135
NspI RCATGY 2 cut(s) 1210, 1419
PaeI GCATGC 1 cut(s) 1210
PaeR7I CTCGAG 2 cut(s) 846, 1331
PagI TCATGA 1 cut(s) 1222
PciI ACATGT 1 cut(s) 1415
PciSI GCTCTTC 1 cut(s) 1371
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 1 cut(s) 236
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 3 cut(s) 347, 557, 581
PflFI GACNNNGTC 1 cut(s) 88
PkrI GCNGC 5 cut(s) 55, 58, 193, 674, 1442
PleI GAGTC 5 cut(s) 95, 403, 475, 838, 955
PpsI GAGTC 5 cut(s) 95, 403, 475, 838, 955
PscI ACATGT 1 cut(s) 1415
Psp124BI GAGCTC 1 cut(s) 666
Psp6I CCWGG 2 cut(s) 275, 1199
PspGI CCWGG 2 cut(s) 275, 1199
PstI CTGCAG 1 cut(s) 1442
PsyI GACNNNGTC 1 cut(s) 88
RsaI GTAC 1 cut(s) 15
RsaNI GTAC 1 cut(s) 14
SacI GAGCTC 1 cut(s) 666
SacII CCGCGG 1 cut(s) 456
SapI GCTCTTC 1 cut(s) 1371
SaqAI TTAA 2 cut(s) 753, 1097
SatI GCNGC 5 cut(s) 54, 57, 192, 673, 1441
Sau3AI GATC 4 cut(s) 74, 112, 156, 251
SchI GAGTC 5 cut(s) 95, 404, 475, 838, 955
ScrFI CCNGG 3 cut(s) 277, 385, 1201
SduI GDGCHC 2 cut(s) 141, 666
SfcI CTRYAG 2 cut(s) 1189, 1438
Sfr274I CTCGAG 2 cut(s) 846, 1331
Sfr303I CCGCGG 1 cut(s) 456
SgrBI CCGCGG 1 cut(s) 456
SlaI CTCGAG 2 cut(s) 846, 1331
SmlI CTYRAG 2 cut(s) 846, 1331
SmoI CTYRAG 2 cut(s) 846, 1331
SphI GCATGC 1 cut(s) 1210
SsiI CCGC 7 cut(s) 54, 192, 204, 409, 453, 455, 672
SstI GAGCTC 1 cut(s) 666
StyD4I CCNGG 3 cut(s) 275, 383, 1199
TaaI ACNGT 2 cut(s) 65, 229
TaiI ACGT 2 cut(s) 24, 1179
TaqI TCGA 7 cut(s) 111, 124, 186, 250, 729, 847, 1332
TatI WGTACW 1 cut(s) 13
TauI GCSGC 3 cut(s) 56, 194, 675
TfiI GAWTC 3 cut(s) 347, 557, 581
Tru1I TTAA 2 cut(s) 753, 1097
Tru9I TTAA 2 cut(s) 753, 1097
TscAI CASTG 1 cut(s) 234
TseFI GTSAC 3 cut(s) 436, 1109, 1135
TseI GCWGC 2 cut(s) 56, 1440
Tsp45I GTSAC 3 cut(s) 436, 1109, 1135
TspDTI ATGAA 2 cut(s) 234, 713
TspRI CASTG 1 cut(s) 234
Tth111I GACNNNGTC 1 cut(s) 88
XagI CCTNNNNNAGG 1 cut(s) 861
XapI RAATTY 6 cut(s) 465, 503, 782, 1061, 1283, 1317
XceI RCATGY 2 cut(s) 1210, 1419
XhoI CTCGAG 2 cut(s) 846, 1331
XmnI GAANNNNTTC 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.