FvH4_3g33260

exonuclease V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002756
Physical Location & Seq
Forward (+)
28666007 .. 28670358
4352 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g33260.t1

Sequence Viewer

Length: 1164 bp
ATGACGGAGTCACGACTCCTCCTCCTCTCGGTGCCCGAAATCCCAATAGAGATTGTGAGCGACGAAGAGATGGCTCTGCTCGAAGCCGCCTTGGTCTCCGCTCGCTCTGTATTTCGATCCTCCTCTCAATCATCTCACTTCCCTTCCAACCTCGATAGGCCCCTTCACTCCATCACCCTCCTTTCCAATTCCAAAAGGAGCCTTTCGGGTTGCTCCCAACCCGATATCGAGGATTCGGGCGACTTCAACAAGAAGAAGACCCGGTCGGGTGAATCGTTATTGGACCGGTTCAGGAACAACAAGGCCTGGTCCGCCACCGATTTTACTAGCACGGAATGGTGTGAGAAACAAAAAGAGTTTGGTCTCCTCAATGGTAAGAGAATAGTCACTCAGGCTATGAAAACTGGTCAAGAGCGCCATGCAAAACTCGAAGAAGAGGTTGTTACAAGAGTTAAAGTTGATGTCAATTCAGAAGAAGATTATTGGGCTCTGAGGCTTTTGAGTTTTATTACCGGCATAAATCAATTATATTTTGATGGATTGACTCGAGAGCTGTTTCTTATAGGTTTTGGAGAAGGTATATGGATGGTGGGAGTGATTGATGAGGTTCAAATGCCGGCAACAGAAACCCAGAGAAATCCTTTATTAGTTGACACAAAGACTCGTGTGAAAGATACACTTCCTTCCGAACCACAAAGAAGGAATGGAAGGTTTCAGTTGATGTTCTACAAGCACTTGTGGGATATCTTAGTTACTTTTAGATTCCCCTCCGACCAATTCTATGATCATTTTTCCTTAAATCCTGATTGTGAGTTATCTGAAGAAATCAGGGAGGTCATGGCTAAATCAGGGTGTCCAGCAAAGACCCTTGATGACGTGGTGAATTACTATAAAAATACCTGGATCACGCTACCCCTTGCTGATGACCAACTTCTATTAAGATATGAGTTACAAAAAGACCATTCAGTAATCGGTGAAGATGAATTTCCTTACAACTCTGATGGGTTTAAGAAGCAACTTCAGTTTTCTCTTGAGTTTTGGCAAGGAAAGCGAGAAGCCAGTTACATTCCCGTGGAAGAGCGATGGAAATGCAACCTCTGTCATTTTACTTCCAATTGTCCTGCAGCTCCTGCTAAAAGCACTAGTAGCAATCTGCCTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo5

Protein Analysis

388

Amino Acids

44.45

Weight (kDa)

5.31

Isoelectric Point (pI)

53.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo5 PF09810 92 - 208 2.2e-31 Exonuclease V - a 5' deoxyribonuclease
Exo5 PF09810 209 - 283 1.1e-08 Exonuclease V - a 5' deoxyribonuclease
Exo5 PF09810 306 - 373 6.4e-13 Exonuclease V - a 5' deoxyribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 31
AccBSI CCGCTC 1 cut(s) 101
AciI CCGC 3 cut(s) 87, 99, 312
AclWI GGATC 2 cut(s) 111, 911
AcsI RAATTY 1 cut(s) 983
AcuI CTGAAG 2 cut(s) 840, 1004
AfiI CCNNNNNNNGG 1 cut(s) 28
AgeI ACCGGT 1 cut(s) 285
AgsI TTSAA 2 cut(s) 247, 611
AhlI ACTAGT 1 cut(s) 1142
AjiI CACGTC 1 cut(s) 877
AjnI CCWGG 2 cut(s) 305, 899
AluBI AGCT 2 cut(s) 553, 1127
AluI AGCT 2 cut(s) 553, 1127
Alw26I GTCTC 2 cut(s) 100, 368
AlwI GGATC 2 cut(s) 111, 911
AlwNI CAGNNNCTG 1 cut(s) 1130
Ama87I CYCGRG 1 cut(s) 546
AoxI GGCC 2 cut(s) 158, 303
ApeKI GCWGC 1 cut(s) 1124
ApoI RAATTY 1 cut(s) 983
AsiGI ACCGGT 1 cut(s) 285
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 3 cut(s) 159, 283, 309
AsuC2I CCSGG 1 cut(s) 262
AsuHPI GGTGA 4 cut(s) 166, 281, 892, 986
AvaI CYCGRG 1 cut(s) 546
AvaII GGWCC 2 cut(s) 283, 309
BaeGI GKGCMC 1 cut(s) 36
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 490
BauI CACGAG 1 cut(s) 663
BbsI GAAGAC 1 cut(s) 263
BbvI GCAGC 1 cut(s) 1136
BccI CCATC 6 cut(s) 64, 179, 530, 580, 995, 1077
BcgI CGANNNNNNTGC 2 cut(s) 1071, 1105
BciT130I CCWGG 2 cut(s) 307, 901
BclI TGATCA 1 cut(s) 784
BcnI CCSGG 1 cut(s) 262
BcoDI GTCTC 2 cut(s) 100, 368
BcuI ACTAGT 1 cut(s) 1142
BfaI CTAG 3 cut(s) 327, 1143, 1162
BfmI CTRYAG 1 cut(s) 1122
BfoI RGCGCY 1 cut(s) 418
BisI GCNGC 2 cut(s) 87, 1125
BlsI GCNGC 2 cut(s) 88, 1126
Bme1390I CCNGG 3 cut(s) 262, 307, 901
Bme18I GGWCC 2 cut(s) 283, 309
BmeT110I CYCGRG 1 cut(s) 546
BmgBI CACGTC 1 cut(s) 877
BmgT120I GGNCC 3 cut(s) 159, 283, 309
BmiI GGNNCC 3 cut(s) 33, 161, 200
BmrFI CCNGG 3 cut(s) 262, 307, 901
BpiI GAAGAC 1 cut(s) 263
BpuEI CTTGAG 1 cut(s) 1052
BpuMI CCSGG 1 cut(s) 262
BsaI GGTCTC 2 cut(s) 100, 368
BsaJI CCNNGG 2 cut(s) 90, 1071
BsaWI WCCGGW 1 cut(s) 285
BsaXI ACNNNNNCTCC 3 cut(s) 6, 33, 36
Bsc4I CCNNNNNNNGG 1 cut(s) 28
Bse118I RCCGGY 3 cut(s) 285, 512, 616
Bse1I ACTGG 2 cut(s) 409, 1059
BseBI CCWGG 2 cut(s) 307, 901
BseDI CCNNGG 2 cut(s) 90, 1071
BseGI GGATG 1 cut(s) 591
BseLI CCNNNNNNNGG 1 cut(s) 28
BseMII CTCAG 2 cut(s) 404, 482
BseNI ACTGG 2 cut(s) 409, 1059
BseRI GAGGAG 5 cut(s) 8, 11, 14, 112, 356
BseSI GKGCMC 1 cut(s) 36
BseXI GCAGC 1 cut(s) 1136
Bsh1285I CGRYCG 1 cut(s) 266
BshFI GGCC 2 cut(s) 160, 305
BshNI GGYRCC 1 cut(s) 31
BshTI ACCGGT 1 cut(s) 285
BsiEI CGRYCG 1 cut(s) 266
BsiHKCI CYCGRG 1 cut(s) 546
BsiSI CCGG 4 cut(s) 262, 286, 513, 617
BslI CCNNNNNNNGG 1 cut(s) 28
BsmAI GTCTC 2 cut(s) 100, 368
BsnI GGCC 2 cut(s) 160, 305
Bso31I GGTCTC 2 cut(s) 100, 368
BsoBI CYCGRG 1 cut(s) 546
Bsp1286I GDGCHC 2 cut(s) 36, 490
Bsp143I GATC 3 cut(s) 116, 784, 903
BspACI CCGC 3 cut(s) 87, 99, 312
BspANI GGCC 2 cut(s) 160, 305
BspCNI CTCAG 2 cut(s) 403, 483
BspLI GGNNCC 3 cut(s) 33, 161, 200
BspMAI CTGCAG 1 cut(s) 1126
BspPI GGATC 2 cut(s) 111, 911
BspQI GCTCTTC 1 cut(s) 1071
BspT107I GGYRCC 1 cut(s) 31
BspTNI GGTCTC 2 cut(s) 100, 368
BsrBI CCGCTC 1 cut(s) 101
BsrFI RCCGGY 3 cut(s) 285, 512, 616
BsrI ACTGG 2 cut(s) 409, 1059
BssAI RCCGGY 3 cut(s) 285, 512, 616
BssECI CCNNGG 2 cut(s) 90, 1071
BssMI GATC 3 cut(s) 116, 784, 903
BssSI CACGAG 1 cut(s) 663
BssT1I CCWWGG 1 cut(s) 90
Bst2BI CACGAG 1 cut(s) 663
Bst2UI CCWGG 2 cut(s) 307, 901
Bst6I CTCTTC 3 cut(s) 60, 429, 1071
BstAPI GCANNNNNTGC 1 cut(s) 1130
BstC8I GCNNGC 2 cut(s) 103, 618
BstDEI CTNAG 3 cut(s) 390, 491, 748
BstDSI CCRYGG 1 cut(s) 1071
BstF5I GGATG 1 cut(s) 591
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 3 cut(s) 119, 787, 906
BstMAI GTCTC 2 cut(s) 100, 368
BstMBI GATC 3 cut(s) 116, 784, 903
BstMCI CGRYCG 1 cut(s) 266
BstMWI GCNNNNNNNGC 4 cut(s) 311, 1048, 1130, 1146
BstNI CCWGG 2 cut(s) 307, 901
BstSCI CCNGG 3 cut(s) 260, 305, 899
BstSFI CTRYAG 1 cut(s) 1122
BstSLI GKGCMC 1 cut(s) 36
BstV1I GCAGC 1 cut(s) 1136
BstV2I GAAGAC 1 cut(s) 263
BsuRI GGCC 2 cut(s) 160, 305
BtgI CCRYGG 1 cut(s) 1071
BtgZI GCGATG 1 cut(s) 1096
BtrI CACGTC 1 cut(s) 877
BtsCI GGATG 1 cut(s) 591
Cac8I GCNNGC 2 cut(s) 103, 618
CaiI CAGNNNCTG 1 cut(s) 1130
CfoI GCGC 1 cut(s) 417
Cfr10I RCCGGY 3 cut(s) 285, 512, 616
Cfr13I GGNCC 3 cut(s) 159, 283, 309
CspAI ACCGGT 1 cut(s) 285
CviAII CATG 2 cut(s) 419, 838
DdeI CTNAG 3 cut(s) 390, 491, 748
DpnI GATC 3 cut(s) 118, 786, 905
DpnII GATC 3 cut(s) 116, 784, 903
Eam1104I CTCTTC 3 cut(s) 60, 429, 1071
EarI CTCTTC 3 cut(s) 60, 429, 1071
EciI GGCGGA 1 cut(s) 301
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 1 cut(s) 305
Eco24I GRGCYC 1 cut(s) 490
Eco31I GGTCTC 2 cut(s) 100, 368
Eco32I GATATC 2 cut(s) 226, 745
Eco47I GGWCC 2 cut(s) 283, 309
Eco57I CTGAAG 2 cut(s) 840, 1004
Eco88I CYCGRG 1 cut(s) 546
EcoO109I RGGNCCY 1 cut(s) 159
EcoRII CCWGG 2 cut(s) 305, 899
EcoRV GATATC 2 cut(s) 226, 745
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 490
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 2 cut(s) 422, 841
FalI AAGNNNNNCTT 2 cut(s) 663, 695
FatI CATG 2 cut(s) 418, 837
FbaI TGATCA 1 cut(s) 784
Fnu4HI GCNGC 2 cut(s) 87, 1125
FokI GGATG 1 cut(s) 598
FriOI GRGCYC 1 cut(s) 490
Fsp4HI GCNGC 2 cut(s) 87, 1125
FspBI CTAG 3 cut(s) 327, 1143, 1162
GlaI GCGC 1 cut(s) 416
GluI GCNGC 2 cut(s) 87, 1125
HaeII RGCGCY 1 cut(s) 418
HaeIII GGCC 2 cut(s) 160, 305
HapII CCGG 4 cut(s) 262, 286, 513, 617
HhaI GCGC 1 cut(s) 417
Hin1II CATG 2 cut(s) 422, 841
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HinfI GANTC 7 cut(s) 8, 15, 233, 272, 544, 661, 762
HpaII CCGG 4 cut(s) 262, 286, 513, 617
HphI GGTGA 4 cut(s) 166, 281, 892, 986
Hpy166II GTNNAC 1 cut(s) 652
Hpy188I TCNGA 6 cut(s) 472, 492, 688, 772, 820, 1000
Hpy188III TCNNGA 6 cut(s) 12, 292, 410, 548, 803, 1031
Hpy8I GTNNAC 1 cut(s) 652
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 6 cut(s) 153, 173, 569, 693, 693, 702
HpyCH4IV ACGT 1 cut(s) 876
HpyCH4V TGCA 3 cut(s) 422, 1092, 1124
HpyF10VI GCNNNNNNNGC 4 cut(s) 311, 1048, 1130, 1146
HpyF3I CTNAG 3 cut(s) 390, 491, 748
HpySE526I ACGT 1 cut(s) 876
Hsp92II CATG 2 cut(s) 422, 841
HspAI GCGC 1 cut(s) 415
KroI GCCGGC 1 cut(s) 616
KroNI GCCGGC 1 cut(s) 618
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 3 cut(s) 116, 784, 903
LguI GCTCTTC 1 cut(s) 1071
LmnI GCTCC 3 cut(s) 198, 218, 1132
Lsp1109I GCAGC 1 cut(s) 1136
MaeI CTAG 3 cut(s) 327, 1143, 1162
MaeII ACGT 1 cut(s) 876
MaeIII GTNAC 6 cut(s) 9, 385, 442, 751, 948, 1061
MalI GATC 3 cut(s) 118, 786, 905
MbiI CCGCTC 1 cut(s) 101
MboI GATC 3 cut(s) 116, 784, 903
MfeI CAATTG 1 cut(s) 1114
MhlI GDGCHC 2 cut(s) 36, 490
MluCI AATT 7 cut(s) 187, 466, 524, 776, 883, 983, 1114
MlyI GAGTC 4 cut(s) 9, 17, 538, 655
MmeI TCCRAC 2 cut(s) 171, 795
MroNI GCCGGC 1 cut(s) 616
MseI TTAA 4 cut(s) 453, 797, 938, 1008
MslI CAYNNNNRTG 1 cut(s) 1070
MspI CCGG 4 cut(s) 262, 286, 513, 617
MspR9I CCNGG 3 cut(s) 262, 307, 901
MunI CAATTG 1 cut(s) 1114
MvaI CCWGG 2 cut(s) 307, 901
MwoI GCNNNNNNNGC 4 cut(s) 311, 1048, 1130, 1146
NaeI GCCGGC 1 cut(s) 618
NciI CCSGG 1 cut(s) 262
NdeII GATC 3 cut(s) 116, 784, 903
NgoMIV GCCGGC 1 cut(s) 616
NlaIII CATG 2 cut(s) 422, 841
NlaIV GGNNCC 3 cut(s) 33, 161, 200
NmuCI GTSAC 2 cut(s) 9, 385
PaeR7I CTCGAG 1 cut(s) 546
PceI AGGCCT 1 cut(s) 305
PciSI GCTCTTC 1 cut(s) 1071
PcsI WCGNNNNNNNCGW 1 cut(s) 272
PdiI GCCGGC 1 cut(s) 618
PfeI GAWTC 3 cut(s) 233, 272, 762
PflFI GACNNNGTC 2 cut(s) 7, 262
PinAI ACCGGT 1 cut(s) 285
PkrI GCNGC 2 cut(s) 88, 1126
PleI GAGTC 4 cut(s) 9, 16, 538, 655
PpsI GAGTC 4 cut(s) 9, 16, 538, 655
Psp6I CCWGG 2 cut(s) 305, 899
PspGI CCWGG 2 cut(s) 305, 899
PspN4I GGNNCC 3 cut(s) 33, 161, 200
PspPI GGNCC 3 cut(s) 159, 283, 309
PstI CTGCAG 1 cut(s) 1126
PstNI CAGNNNCTG 1 cut(s) 1130
PsyI GACNNNGTC 2 cut(s) 7, 262
RseI CAYNNNNRTG 1 cut(s) 1070
SapI GCTCTTC 1 cut(s) 1071
SaqAI TTAA 4 cut(s) 453, 797, 938, 1008
SatI GCNGC 2 cut(s) 87, 1125
Sau3AI GATC 3 cut(s) 116, 784, 903
Sau96I GGNCC 3 cut(s) 159, 283, 309
SchI GAGTC 4 cut(s) 9, 17, 538, 655
ScrFI CCNGG 3 cut(s) 262, 307, 901
SduI GDGCHC 2 cut(s) 36, 490
SfcI CTRYAG 1 cut(s) 1122
Sfr274I CTCGAG 1 cut(s) 546
SinI GGWCC 2 cut(s) 283, 309
SlaI CTCGAG 1 cut(s) 546
SmiMI CAYNNNNRTG 1 cut(s) 1070
SmlI CTYRAG 2 cut(s) 546, 1031
SmoI CTYRAG 2 cut(s) 546, 1031
SpeI ACTAGT 1 cut(s) 1142
Sse9I AATT 7 cut(s) 187, 466, 524, 776, 883, 983, 1114
SseBI AGGCCT 1 cut(s) 305
SsiI CCGC 3 cut(s) 87, 99, 312
SspMI CTAG 3 cut(s) 327, 1143, 1162
StuI AGGCCT 1 cut(s) 305
StyD4I CCNGG 3 cut(s) 260, 305, 899
StyI CCWWGG 1 cut(s) 90
TaiI ACGT 1 cut(s) 879
TaqI TCGA 6 cut(s) 81, 115, 153, 228, 429, 547
TasI AATT 7 cut(s) 187, 466, 524, 776, 883, 983, 1114
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 3 cut(s) 233, 272, 762
Tru1I TTAA 4 cut(s) 453, 797, 938, 1008
Tru9I TTAA 4 cut(s) 453, 797, 938, 1008
TseFI GTSAC 2 cut(s) 9, 385
TseI GCWGC 1 cut(s) 1124
Tsp45I GTSAC 2 cut(s) 9, 385
TspDTI ATGAA 2 cut(s) 413, 996
TspGWI ACGGA 2 cut(s) 20, 347
Tth111I GACNNNGTC 2 cut(s) 7, 262
VpaK11BI GGWCC 2 cut(s) 283, 309
XapI RAATTY 1 cut(s) 983
XhoI CTCGAG 1 cut(s) 546
XspI CTAG 3 cut(s) 327, 1143, 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.